LOCUS       DAECOD010000001        99824 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.001, whole genome shotgun sequence.
ACCESSION   DAECOD010000001 DAECOD010000000
VERSION     DAECOD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 99824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.001"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="KKO15_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="KKO15_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBD6358959.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="KKO15_000002"
     CDS             complement(1672..2730)
                     /locus_tag="KKO15_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6358960.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="KKO15_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="KKO15_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="KKO15_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="KKO15_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBD6358961.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KKO15_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KKO15_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBD6358962.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KKO15_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KKO15_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBD6358963.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="KKO15_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="KKO15_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBD6358964.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="KKO15_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="KKO15_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBD6358965.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KKO15_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KKO15_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBD6358966.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KKO15_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KKO15_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBD6358967.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KKO15_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KKO15_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBD6358968.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KKO15_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KKO15_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBD6358969.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="KKO15_000013"
     CDS             complement(17699..18322)
                     /locus_tag="KKO15_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBD6358970.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="KKO15_000014"
     CDS             18619..18894
                     /locus_tag="KKO15_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6358971.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="KKO15_000015"
     CDS             complement(18971..19213)
                     /locus_tag="KKO15_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBD6358972.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="KKO15_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="KKO15_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBD6358973.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="KKO15_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="KKO15_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBD6358974.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="KKO15_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="KKO15_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBD6358975.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22591..24077
                     /locus_tag="KKO15_000019"
                     /pseudo
     CDS             22591..24077
                     /locus_tag="KKO15_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24092..24826
                     /gene="ypdB"
                     /locus_tag="KKO15_000020"
     CDS             24092..24826
                     /gene="ypdB"
                     /locus_tag="KKO15_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBD6358976.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24839..25696
                     /locus_tag="KKO15_000021"
     CDS             24839..25696
                     /locus_tag="KKO15_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6358977.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="KKO15_000022"
     CDS             complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="KKO15_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6358978.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="KKO15_000023"
     CDS             complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="KKO15_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD6358979.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="KKO15_000024"
                     /pseudo
     CDS             complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="KKO15_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30354..31601)
                     /locus_tag="KKO15_000025"
     CDS             complement(30354..31601)
                     /locus_tag="KKO15_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBD6358980.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31623..31949)
                     /locus_tag="KKO15_000026"
     CDS             complement(31623..31949)
                     /locus_tag="KKO15_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBD6358981.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32168..33133)
                     /gene="glk"
                     /locus_tag="KKO15_000027"
     CDS             complement(32168..33133)
                     /gene="glk"
                     /locus_tag="KKO15_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBD6358982.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33337..34593
                     /locus_tag="KKO15_000028"
     CDS             33337..34593
                     /locus_tag="KKO15_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBD6358983.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34708..35034
                     /locus_tag="KKO15_000029"
     CDS             34708..35034
                     /locus_tag="KKO15_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD6358984.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35175..36413)
                     /locus_tag="KKO15_000030"
     CDS             complement(35175..36413)
                     /locus_tag="KKO15_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBD6358985.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36749..37951
                     /gene="nupC"
                     /locus_tag="KKO15_000031"
     CDS             36749..37951
                     /gene="nupC"
                     /locus_tag="KKO15_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBD6358986.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38001..39316)
                     /locus_tag="KKO15_000032"
                     /pseudo
     CDS             complement(38001..39316)
                     /locus_tag="KKO15_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(39456..40082)
                     /locus_tag="KKO15_000033"
     CDS             complement(39456..40082)
                     /locus_tag="KKO15_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBD6358987.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRIYLKLAGSVKRAATSVVHTL"
     gene            complement(40290..40365)
                     /locus_tag="KKO15_000034"
     tRNA            complement(40290..40365)
                     /locus_tag="KKO15_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40330..40332),aa:Ala,seq:ggc)
     gene            complement(40405..40480)
                     /locus_tag="KKO15_000035"
     tRNA            complement(40405..40480)
                     /locus_tag="KKO15_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40445..40447),aa:Ala,seq:ggc)
     gene            40701..41060
                     /locus_tag="KKO15_000036"
     CDS             40701..41060
                     /locus_tag="KKO15_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD6358988.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41062..41454
                     /locus_tag="KKO15_000037"
     CDS             41062..41454
                     /locus_tag="KKO15_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD6358989.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="KKO15_000038"
     CDS             complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="KKO15_000038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBD6358990.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43180..43255
                     /locus_tag="KKO15_000039"
     tRNA            43180..43255
                     /locus_tag="KKO15_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43213..43215,aa:Val,seq:tac)
     gene            43300..43375
                     /locus_tag="KKO15_000040"
     tRNA            43300..43375
                     /locus_tag="KKO15_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43333..43335,aa:Val,seq:tac)
     gene            43380..43455
                     /locus_tag="KKO15_000041"
     tRNA            43380..43455
                     /locus_tag="KKO15_000041"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43413..43415,aa:Lys,seq:ttt)
     gene            43486..43611
                     /locus_tag="KKO15_000042"
     ncRNA           43486..43611
                     /ncRNA_class="other"
                     /locus_tag="KKO15_000042"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43731..44072
                     /locus_tag="KKO15_000043"
     CDS             43731..44072
                     /locus_tag="KKO15_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBD6358991.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44063..44989)
                     /locus_tag="KKO15_000044"
     CDS             complement(44063..44989)
                     /locus_tag="KKO15_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6358992.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45079..46077
                     /locus_tag="KKO15_000045"
     CDS             45079..46077
                     /locus_tag="KKO15_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBD6358993.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46074..46292)
                     /locus_tag="KKO15_000046"
     CDS             complement(46074..46292)
                     /locus_tag="KKO15_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBD6358994.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="KKO15_000047"
     CDS             complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="KKO15_000047"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBD6358995.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFVGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="KKO15_000048"
     CDS             complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="KKO15_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBD6358996.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49596..50357
                     /gene="cysZ"
                     /locus_tag="KKO15_000049"
     CDS             49596..50357
                     /gene="cysZ"
                     /locus_tag="KKO15_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBD6358997.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50542..51513
                     /gene="cysK"
                     /locus_tag="KKO15_000050"
     CDS             50542..51513
                     /gene="cysK"
                     /locus_tag="KKO15_000050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBD6358998.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51897..52154
                     /gene="ptsH"
                     /locus_tag="KKO15_000051"
     CDS             51897..52154
                     /gene="ptsH"
                     /locus_tag="KKO15_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBD6358999.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52199..53926
                     /gene="ptsI"
                     /locus_tag="KKO15_000052"
     CDS             52199..53926
                     /gene="ptsI"
                     /locus_tag="KKO15_000052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBD6359000.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53967..54476
                     /gene="crr"
                     /locus_tag="KKO15_000053"
     CDS             53967..54476
                     /gene="crr"
                     /locus_tag="KKO15_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBD6359001.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="KKO15_000054"
                     /gene_synonym="thiJ"
     CDS             complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="KKO15_000054"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBD6359002.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55474..55848
                     /locus_tag="KKO15_000055"
     CDS             55474..55848
                     /locus_tag="KKO15_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBD6359003.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55881..56604
                     /locus_tag="KKO15_000056"
                     /pseudo
     CDS             55881..56604
                     /locus_tag="KKO15_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="KKO15_000057"
     CDS             complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="KKO15_000057"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBD6359004.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSLGAGI"
     gene            complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="KKO15_000058"
     CDS             complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="KKO15_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBD6359005.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="KKO15_000059"
     CDS             complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="KKO15_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBD6359006.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="KKO15_000060"
     CDS             complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="KKO15_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBD6359007.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVTLSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="KKO15_000061"
     CDS             complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="KKO15_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBD6359008.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="KKO15_000062"
     CDS             complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="KKO15_000062"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBD6359009.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62946..63746)
                     /gene="murR"
                     /locus_tag="KKO15_000063"
     CDS             complement(62946..63746)
                     /gene="murR"
                     /locus_tag="KKO15_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBD6359010.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63910..64806
                     /gene="murQ"
                     /locus_tag="KKO15_000064"
     CDS             63910..64806
                     /gene="murQ"
                     /locus_tag="KKO15_000064"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBD6359011.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64810..66234
                     /gene="murP"
                     /locus_tag="KKO15_000065"
                     /pseudo
     CDS             64810..66234
                     /gene="murP"
                     /locus_tag="KKO15_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="KKO15_000066"
     CDS             complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="KKO15_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBD6359012.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67409..67984)
                     /locus_tag="KKO15_000067"
     CDS             complement(67409..67984)
                     /locus_tag="KKO15_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBD6359013.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68045..68494)
                     /locus_tag="KKO15_000068"
     CDS             complement(68045..68494)
                     /locus_tag="KKO15_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBD6359014.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68481..68906)
                     /locus_tag="KKO15_000069"
     CDS             complement(68481..68906)
                     /locus_tag="KKO15_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD6359015.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69120..69989
                     /gene="amiA"
                     /locus_tag="KKO15_000070"
     CDS             69120..69989
                     /gene="amiA"
                     /locus_tag="KKO15_000070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBD6359016.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69993..70892
                     /gene="hemF"
                     /locus_tag="KKO15_000071"
     CDS             69993..70892
                     /gene="hemF"
                     /locus_tag="KKO15_000071"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBD6359017.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="KKO15_000072"
                     /pseudo
     CDS             complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="KKO15_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="KKO15_000073"
     CDS             complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="KKO15_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBD6359018.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="KKO15_000074"
     CDS             complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="KKO15_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBD6359019.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74350..75141
                     /locus_tag="KKO15_000075"
     CDS             74350..75141
                     /locus_tag="KKO15_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359020.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75861..76256
                     /locus_tag="KKO15_000076"
     CDS             75861..76256
                     /locus_tag="KKO15_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6359021.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSREKKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76274..76537
                     /locus_tag="KKO15_000077"
     CDS             76274..76537
                     /locus_tag="KKO15_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359022.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76560..77102
                     /locus_tag="KKO15_000078"
     CDS             76560..77102
                     /locus_tag="KKO15_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359023.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77241..77528
                     /locus_tag="KKO15_000079"
     CDS             77241..77528
                     /locus_tag="KKO15_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359024.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77567..77773
                     /locus_tag="KKO15_000080"
     CDS             77567..77773
                     /locus_tag="KKO15_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359025.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78085..78570
                     /locus_tag="KKO15_000081"
     CDS             78085..78570
                     /locus_tag="KKO15_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6359026.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78551..78829
                     /locus_tag="KKO15_000082"
     CDS             78551..78829
                     /locus_tag="KKO15_000082"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359027.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78822..80282
                     /locus_tag="KKO15_000083"
     CDS             78822..80282
                     /locus_tag="KKO15_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6359028.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80316..81125
                     /gene="murI"
                     /locus_tag="KKO15_000084"
     CDS             80316..81125
                     /gene="murI"
                     /locus_tag="KKO15_000084"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD6359029.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81244..81938)
                     /locus_tag="KKO15_000085"
     CDS             complement(join(81244..81692,81692..81938))
                     /locus_tag="KKO15_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6359030.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82457..83830)
                     /gene="eutA"
                     /locus_tag="KKO15_000086"
     CDS             complement(82457..83830)
                     /gene="eutA"
                     /locus_tag="KKO15_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBD6359031.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83805..84284)
                     /gene="eutP"
                     /locus_tag="KKO15_000087"
     CDS             complement(83805..84284)
                     /gene="eutP"
                     /locus_tag="KKO15_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBD6359032.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84297..84632)
                     /gene="eutS"
                     /locus_tag="KKO15_000088"
     CDS             complement(84297..84632)
                     /gene="eutS"
                     /locus_tag="KKO15_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBD6359033.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84925..87204)
                     /gene="maeB"
                     /locus_tag="KKO15_000089"
                     /pseudo
     CDS             complement(84925..87204)
                     /gene="maeB"
                     /locus_tag="KKO15_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
     gene            87493..88429
                     /gene="tal"
                     /locus_tag="KKO15_000090"
                     /pseudo
     CDS             87493..88429
                     /gene="tal"
                     /locus_tag="KKO15_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            88449..90452
                     /gene="tkt"
                     /locus_tag="KKO15_000091"
     CDS             88449..90452
                     /gene="tkt"
                     /locus_tag="KKO15_000091"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD6359034.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90555..91598)
                     /locus_tag="KKO15_000092"
     CDS             complement(90555..91598)
                     /locus_tag="KKO15_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBD6359035.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91724..92299)
                     /gene="nudK"
                     /locus_tag="KKO15_000093"
     CDS             complement(91724..92299)
                     /gene="nudK"
                     /locus_tag="KKO15_000093"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBD6359036.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92367..94346)
                     /gene="aegA"
                     /locus_tag="KKO15_000094"
     CDS             complement(92367..94346)
                     /gene="aegA"
                     /locus_tag="KKO15_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBD6359037.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94552..96269
                     /gene="narQ"
                     /locus_tag="KKO15_000095"
                     /pseudo
     CDS             94552..96269
                     /gene="narQ"
                     /locus_tag="KKO15_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96471..99584
                     /gene="acrD"
                     /locus_tag="KKO15_000096"
     CDS             96471..99584
                     /gene="acrD"
                     /locus_tag="KKO15_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBD6359038.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99683..99742)
                     /gene="ypfM"
                     /locus_tag="KKO15_000097"
     CDS             complement(99683..99742)
                     /gene="ypfM"
                     /locus_tag="KKO15_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBD6359039.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        25563 a        25442 c        24030 g        24789 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taatcccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac atcatgattt ttatagcttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctga cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgtggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc atttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaatcacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgctgg atatcgccaa caaaacgcta ccgctgtttc
    23041 gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat tatccgcgat gacattcacg
    23101 ccgatgcggt ggcgattact aataccgatc atgtgctggc ctatgttggc gtgggtgaac
    23161 ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg atgaattacg
    23221 gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt cattccatgc
    23281 tggtgatccc attgtgggag aaaggggtcg tgaccggaac gctgaaaatt tactactgcc
    23341 acgcgcatca gatcacctcg tcattacagg aaatggcggt cggtctgtcg caaatcatct
    23401 ccacgcaact ggaggtttca cgcgccgagc agctacgtga aatggcaaat aaggcagagc
    23461 ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg aacgctattt
    23521 catcgtcaat ccgtctgaat ccggataccg ctcgccagtt gatctttaat ctgtcgcgtt
    23581 atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt
    23641 atcaaattaa agactatatt gccattgagc aggcccgctt tggcgacaag ctgacggtta
    23701 tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc cagccgttgg
    23761 tggagaacgc cattgtccac ggtattcagc cttgcaaagg taaaggcgtt gtcaccatca
    23821 gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg agataccggg cacggcatcg
    23881 ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa attggcctgc
    23941 tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc cgccgcctgg
    24001 agccggggac ggaaattgcg ttttacattc ctaaccaacg caccccagtc gcctcacagg
    24061 ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg aagacgaatt
    24121 cctggcacaa caggaactga gctggctaat taaagagcac agccagatgg agattgtcgg
    24181 cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg tcgacgccat
    24241 ttttctggat atcaatattc cgtcgctgga tggcgtgttg ctggcgcaaa acatcagcca
    24301 gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg cggtagaagc
    24361 gtttgaactg gaggcgtttg actacattct caaaccgtat caggagtcac gtattaccgg
    24421 gatgctgcaa aaactggaag cggcctggca acaacagcag accagcagta cgcctgccgc
    24481 gacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa taatcgtcac
    24541 gccaattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg tctatacgcg
    24601 gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac tgccgccgtc
    24661 gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatac gcgaaatcga
    24721 accgtggttt aataacacct acattctgcg actgaaagat ctggattttg aagtgccggt
    24781 cagccgcagc aaagtgaaag aatttcgcca gttaatgcat ctttaatcgg gaactttcat
    24841 gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca gcggcctagt
    24901 gcttaacccg caactactgg ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt
    24961 gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa
    25021 cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa atgctgttta ttccggcacg
    25081 cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc
    25141 gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc atcctgctcg
    25201 ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta ccgccctcac
    25261 ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt taagtttgct
    25321 gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat tcacaaccgc gcggcgattt
    25381 tctctatcac agcatttgta actgggttca ggataattat gcccagccgc tcacccgcga
    25441 gagcgtggcg cagtttttta atatcacgcc caatcatctg tcaaaactgt ttgctcagca
    25501 tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga atggcgaagg cgaggatgat
    25561 tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt ttccggatag
    25621 cgactatttt tgtcgcgttt tccggcgtca gtttagtctg acgccgggag agtacagcgc
    25681 ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca ggatactttg
    25741 tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt tacgcgccag
    25801 ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca gcatgatcac
    25861 cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc gggcaatgct
    25921 gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgctacgc caaaaccaac
    25981 gccggtggta acaatctctt cccgctgcca gacatcttct tccagctcaa acggatgttc
    26041 agtgcgcccg ttaacgccga ggttgccgca caggaactgg atcgcctgct ctttattgct
    26101 aaaatcctga tcaacaaaga tattctccag cgccagcagt gggcgaacgt cttcttccgg
    26161 cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg cggcattcac atgcctgacg
    26221 cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca ccgccggaat
    26281 acgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg gcagataacg
    26341 gctttcaccg cccagttcac cgcaaatgcc tacccatttg ccccgctgat gcgcagtggt
    26401 aactatttgc tgcaacatgc gcaggaacga tggcgtaatc gggttatata gcggcgatac
    26461 gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg agccgatact
    26521 gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg gcacttccac
    26581 catgatccca agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat
    26641 cgctttttgg atctcgcctt tcacccataa gatctgatcg aggctgtgaa ccatcgggat
    26701 catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa
    26761 caggccagca aattccgggt aaatacgtac cgcgcgatag ccgaggaacg ggttctcttc
    26821 ctggggaata ttcagataag gaatgctttt atcgccgccg atgtccatcg tgcggaagat
    26881 aatcggcttg tcgcccgccg ccagtagcac ctgctggtag gcttcaaatt gctcctgctc
    26941 gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc cgacaccttc
    27001 cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg ccgatattcg ccgcaatatc
    27061 aatacgcttg ttatcacggg aataggccag ctgcgcggca gcctgcgcct gttgtttttg
    27121 gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt cattcgggtt
    27181 aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat aacgggcaat
    27241 cgcatccagc ggcaagccac tcagtaccgg gatcgccgag gcacgggcca gaatcagtgt
    27301 atgcgaggtg cgcccggttt tctccagaat catacccgca agatttttca aatcaaggct
    27361 caaaaactgg cttggggtta aatcttcagc caccagaatg gtcggttttt ccagcaccag
    27421 attgttgcgc ggcttcagtt ccggccaggt gatatgcagc aactgttcgc tgatatcgcg
    27481 aatgtcgctg acccgctcgc gcagataatc gctggcagaa gcagatagtt tggcgcaaac
    27541 ctgctccata ttgctgatga tcgccgcccc cagtccctga tgctgttctg tcatcaggcg
    27601 acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgagcgc tgaggatagt
    27661 tttgctttcg ccgtcacgct cacgcaattg ctgattcagt tgctcggcaa gcgttgccag
    27721 gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat aactgtcgag
    27781 gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc
    27841 gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg ccgtcagttc
    27901 tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc accaggatgt actcttccag
    27961 cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac tgtcattaaa
    28021 taaggtgcca gttccaatca acgccagcga gcttttagca tcagcctttg cgttctggcg
    28081 atggttgata aaggtgattt cgctttgcca ctggctgcac tgttctttaa gttcccacgc
    28141 cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta acataaccgc
    28201 tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct cacgcgtaag
    28261 acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc aatcggcaat
    28321 cgatgccgcg caatgtccgt ggcgggtggc aggccccatt accaatgtgg gtacgccagt
    28381 accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat ccgcctgcaa
    28441 cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg gcggcgcaat
    28501 cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat ggttagccgc
    28561 gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga cgtccggcga
    28621 caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg aacttgccac
    28681 cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag
    28741 atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag ggaggacctg
    28801 aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc
    28861 ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct gccggtcgcc
    28921 gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg gctgcagctg
    28981 gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt cgcgggagat
    29041 gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct tcggacctgt
    29101 cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt ctttttgcag
    29161 gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg cgatcgcatc
    29221 tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt gagcaacact
    29281 gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac aacatcttcg
    29341 atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag caacatgcct
    29401 ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac ttcaatgccg
    29461 atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt aatgacccgg
    29521 cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga gattgccgcg
    29581 agctgtgcct gcaggacaat ctgatagacg tcaaacagcg ggtgagattc ggcgctcacc
    29641 ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat acagcgcacc
    29701 aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg
    29761 cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg
    29821 ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat
    29881 aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc ggattatctc
    29941 cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc
    30001 attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc
    30061 gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt
    30121 cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa cgaaatagcg
    30181 cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg atccggtgga
    30241 gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat ccagttgttg
    30301 caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg
    30361 ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac caccatcagc
    30421 gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaggtt agtgaccagc
    30481 ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca gacagctgcg
    30541 gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg gctttccagc
    30601 gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc ggtcttaccc
    30661 tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc aataatggtc
    30721 gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt gaccggtaat
    30781 acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat cgggccacca
    30841 aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct
    30901 gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg agtgatgacg
    30961 tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg
    31021 aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg
    31081 gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc
    31141 cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaacccg
    31201 ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac aaatgcagca
    31261 aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag ccaggcaaat
    31321 ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg
    31381 atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc gaggatcaca
    31441 ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt
    31501 tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat
    31561 gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa
    31621 tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt
    31681 ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ttcgttatct
    31741 tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc ctgcgccgtc
    31801 aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct
    31861 accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagctaggcc cattgggcag
    31921 gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga tagttgacga
    31981 gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga
    32041 gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa
    32101 aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag
    32161 gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac
    32221 ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct ttaaagcgcc
    32281 ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg aagcgcggca
    32341 cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc
    32401 caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg gtgcagctgt
    32461 cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcag
    32521 ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg cgctccgccg
    32581 aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct tcactattcg
    32641 gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga
    32701 ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc ttaccttcga
    32761 ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg atcgccatcg
    32821 atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt
    32881 cggcaattga gaacgcccaa gtatggttgg tcattgccac ccagtcaccg gtaattgggc
    32941 aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa
    33001 tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac
    33061 cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg
    33121 catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta
    33181 aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg
    33241 gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg
    33301 caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc
    33361 atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt
    33421 gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact
    33481 ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct aacgggtatt
    33541 gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa
    33601 ccgctgatcg gcgcaccggt tccgccctct gcgctacctg gacttatcgt agcattaatt
    33661 ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc
    33721 gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact
    33781 attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg
    33841 atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg
    33901 ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt
    33961 tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt
    34021 gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc acggttgcac
    34081 gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg atttattctc
    34141 ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga tgagatgcag
    34201 cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa
    34261 cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca
    34321 gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgttccagcg
    34381 gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg
    34441 ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt
    34501 atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt
    34561 ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt tatgatttaa
    34621 gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga
    34681 cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg
    34741 atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc atcgatcaaa
    34801 ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgagat
    34861 cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg tcatgataat
    34921 ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg
    34981 cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca ttaaacgcgt
    35041 cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg
    35101 gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata gtgccagatg
    35161 cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat attcagcgcc
    35221 acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt caccagatcg
    35281 cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca
    35341 aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc catcagaatg
    35401 acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat atggaagcga
    35461 atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga ggacagtccg
    35521 gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag cggttgcagc
    35581 gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc
    35641 gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc gatagccaca
    35701 tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc
    35761 gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc tgctgccagg
    35821 aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag ctgcgccagg
    35881 ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa cagtaacccg
    35941 ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat caggaaagtc
    36001 gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag tttaaaaccg
    36061 atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc tgcctgaacc
    36121 caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc cagattttta
    36181 ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat caggttggcc
    36241 caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat attggtcgca
    36301 aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat taatgcgagc
    36361 ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt catcttgtgc
    36421 ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga ttatcaaatt
    36481 catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac gaaaagattg
    36541 atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt tcagcttcca
    36601 tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc acaggacgtc
    36661 attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa
    36721 tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact
    36781 tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg
    36841 ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga
    36901 cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc
    36961 caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt
    37021 cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat
    37081 tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag tcaacggtat
    37141 gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt cagaaaactt
    37201 tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc
    37261 cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc
    37321 gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct
    37381 gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga
    37441 aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat
    37501 tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt
    37561 tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc tgggctacat
    37621 cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag
    37681 tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat
    37741 tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag tttccttcgc
    37801 taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg
    37861 taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg tgagtgtgct
    37921 gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc ggggatcatt
    37981 ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat caaataccct
    38041 tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt ctcgacaaac
    38101 tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct cacaatcatc
    38161 gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt gatgatatca
    38221 gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc atcaatcgca
    38281 atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc tgcgttagaa
    38341 aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg ataacgctta
    38401 aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg cattaaattg
    38461 actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt caacaaggat
    38521 tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag gggcagaaac
    38581 ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc atcataacct
    38641 tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg atctaacgcc
    38701 gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac catctcctct
    38761 ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag ccagcttaac
    38821 tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca ggcagcacca
    38881 tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag gatgttaacc
    38941 atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag ttcactaccc
    39001 ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg gcaaagccgt
    39061 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct
    39121 cttgctccgg cgcttgttcc aacgcacgaa agttgggtag gagcgttaac ggatctgtta
    39181 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    39241 agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg
    39301 tttccacccc ttgcaaaaag ttctggttgt aattcagaag gcaaagcgtt gaaacagccc
    39361 aggtgagatt taaaaacgga tagcgaagat taccgacccc gagggtaaag atgatgaaga
    39421 aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac aacagaagta
    39481 gcagcacgct taacgctgcc agccagctta aggtaaatgc gagtgagata gtagaaaagc
    39541 atgttgtaaa tcagcactgc ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca
    39601 tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga
    39661 tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag
    39721 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    39781 caggcacaca acacgacaaa cgtctggctg ccgagcatga ccgcagtctg taaaaaagag
    39841 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc
    39901 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    39961 taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    40021 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    40081 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    40141 atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat gctttcacca
    40201 ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    40261 cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac
    40321 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    40381 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    40441 gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag
    40501 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcaacgtgta
    40561 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    40621 gttattcatg tcacggtttc ctgtaaagtg gtgttataaa atgaactact aatagaccca
    40681 catacattca gggaattgtt atgttcaagg agaggatgac accagatgaa cttgccagac
    40741 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt
    40801 caccaaaacc tggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc
    40861 gtgaatatat tcgcaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg
    40921 gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac
    40981 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    41041 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    41101 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggaccagaga aaaaggctgg
    41161 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    41221 caagtttgcg agtttattca gaacacgcca gccttccata acacaccaat gttgatggaa
    41281 gctgaagaga gaattgctga atacgctcct ggagcacgcg ccccggcgta tcgacaaatc
    41341 atcaacgcga tagataatat gacagacatt gagcaggaaa aagtcgcgca atttttgtca
    41401 cgtgaaggaa ttcgcaattt cctcgcccgt ctcgatattg acgaatcagc ataaataaaa
    41461 aacggcagga tattatctcc tgccgtttat ctttttacac gctaattact gctgattttc
    41521 gcgttcagca ataaaatcca gcgctttgtt gatacgctcg atactgcggg tcttaccaat
    41581 tgcgtgaacg gtaacatcca gtgctggaga ctgccccgca ccggttacgg cgacacgcag
    41641 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    41701 atgaacgttt tcagcggtcc agtcagtaat cgcggccagt ttgtcacgaa ccacttccag
    41761 cggctgacgc gctaccggac gcagatgttt tttcgccgca tcggcatcga actcagcaaa
    41821 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    41881 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    41941 aatgtgccac tgtaagtgag tagcaacata ctccggcggc agcgcgttaa tgtagtgatg
    42001 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcaa
    42061 agtgaagtat ttgatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    42121 cagacgcacc agatagttca gcagtgcttc tggcaaataa ccgtcatcac gatactgcat
    42181 tacgctgact gccccgtgac gtttggacag ttttttaccg tcatcgccat tgatcataga
    42241 aacgtgcgcg taaaccggca ccggcgcttt cagggcctta agaatgttga tctggcgcgg
    42301 cgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    42361 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa taagatcgtc
    42421 cagttcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    42481 ttcctgcggg ttagcaaaac gtacaacaca cggttcatca tcagcatgat gctcatggct
    42541 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    42601 cgcttccagg cgctctttag agcaatagca tttataagca gtgccctctt ccagcatctg
    42661 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    42721 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    42781 ctcaagatcg gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag
    42841 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    42901 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    42961 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    43021 tcgggtttct accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    43081 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    43141 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    43201 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccaactac
    43261 tttatgtagt ctccgccgtg tagcaagaaa ttgagaagtg ggtgattagc tcagctggga
    43321 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccacttcg
    43381 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    43441 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac
    43501 tcaaagattc gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg
    43561 cggcgacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgcaaaaa
    43621 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    43681 ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta
    43741 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    43801 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa
    43861 ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga
    43921 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaaa cagcaggcag
    43981 aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    44041 aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc cgaccagcca
    44101 ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    44161 aacggttctt cctgcgcacc agcatcaact ggcgttcaac caccggcgta atgcgcttaa
    44221 ccaccagtgg gctaccttca ggcaatggta gcgccagtgc aggaagaata ctgatgccga
    44281 tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg
    44341 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat
    44401 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca
    44461 aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca
    44521 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    44581 tttccatcac ccactgctgc ggacgatcat gcaggacaaa ctgaatatct ggatagcggc
    44641 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    44701 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    44761 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    44821 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    44881 tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct
    44941 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    45001 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    45061 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    45121 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    45181 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    45241 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    45301 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    45361 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    45421 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    45481 gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    45541 cacggtgcaa ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    45601 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    45661 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    45721 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    45781 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    45841 agcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    45901 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    45961 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    46021 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    46081 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    46141 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    46201 tactcctccg gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    46261 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    46321 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    46381 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    46441 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    46501 cacggtttta ccaacaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    46561 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    46621 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    46681 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    46741 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    46801 cgctttttcc agcgcgttaa ccacgttttg tgccaatttt ggccccatac gctccagtcc
    46861 ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtatggacat attctttttc
    46921 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    46981 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    47041 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa
    47101 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    47161 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    47221 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc
    47281 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    47341 aaaggtcatc tgctcctgcg ccgggaattt aaacgcaacc gcccagcgcg gggcacgcgc
    47401 gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat
    47461 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    47521 cgccgattca caaagcgtta cccgatcgct gaccggcacc ccccactttt taaattgcag
    47581 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    47641 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    47701 tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat
    47761 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    47821 attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc
    47881 ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag
    47941 aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt
    48001 tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt
    48061 atccagtgac agcattggta cttcatggcg tatctggctg aaagctgcca gcggcgcagc
    48121 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    48181 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    48241 atgataaaga tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    48301 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    48361 ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt
    48421 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    48481 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    48541 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    48601 gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat
    48661 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    48721 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    48781 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    48841 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    48901 ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    48961 gttcaggctg cggctgatag gcaggctgct gcaccggctg cgctggacgc ggagcatgtt
    49021 gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat
    49081 aaggcggtag gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag
    49141 ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat
    49201 cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga
    49261 acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg
    49321 cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg
    49381 ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc
    49441 aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct
    49501 gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt
    49561 ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc
    49621 cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc
    49681 ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt
    49741 ggctctttac acagctcgat gtctggatcc cgactttcat gagttacgtt ccggactggc
    49801 tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg
    49861 gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg
    49921 aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca
    49981 tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgtctgg tatctgccgc
    50041 gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg
    50101 tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg
    50161 ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca
    50221 atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca
    50281 tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac
    50341 acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg
    50401 tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata
    50461 tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt
    50521 tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt
    50581 cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa
    50641 tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc
    50701 gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc
    50761 gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca
    50821 gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg
    50881 acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc
    50941 aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac
    51001 gaaaaaacca ccggtccgga aatctgggaa gataccgacg gtcaggttga tgtatttatt
    51061 gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa
    51121 ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag
    51181 gtgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt
    51241 tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa
    51301 gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct
    51361 tctggagcag ctgttgccgc agcgttgaaa ctacaagaag atgaaagctt taccaacaag
    51421 aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc
    51481 gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa
    51541 agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga
    51601 ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg
    51661 ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg
    51721 cataatgctt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat
    51781 tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga
    51841 accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt
    51901 tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt
    51961 ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg
    52021 ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga
    52081 ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg
    52141 cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat
    52201 gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga
    52261 agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga
    52321 acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc
    52381 tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga
    52441 agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga
    52501 cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga
    52561 atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat
    52621 cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc
    52681 tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac
    52741 cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc
    52801 tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga
    52861 tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc
    52921 tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc
    52981 tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt
    53041 tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt
    53101 catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc
    53161 tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga
    53221 gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg
    53281 tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc
    53341 ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc
    53401 actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga
    53461 cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca
    53521 tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct
    53581 ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt
    53641 gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat
    53701 gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga
    53761 cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa
    53821 cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt
    53881 aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg
    53941 cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt
    54001 ccgacgacaa aaaggatacc ggaactattg agatcattgc tccactctct ggcgagatcg
    54061 tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg
    54121 ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct
    54181 ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact
    54241 tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc
    54301 agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag
    54361 ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca
    54421 aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct
    54481 tgccgcagtg aaaaatggcg cccatcggcg ccatttttta tgcttccgcc agcggcggca
    54541 aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt acgcgcaacc
    54601 ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg ataagctgag
    54661 cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg gaaatgacat
    54721 tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca ttaccggagg
    54781 cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg
    54841 cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag atattggggg
    54901 taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga
    54961 cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat aggtcaggat
    55021 ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat gccgttccca
    55081 tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga agcgcacgca
    55141 aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca taatgcggcg
    55201 tattgctcag caataccgtc ggcacggcaa agacattcaa gccgttctgt tttatagcag
    55261 gcaccgcaat gctgttgcct acgcttccgt acactacctg cgactgcacg gcgacgatat
    55321 ccgcctgcag cgccctactc ttatcgttaa acaacaacaa actactcatt taattttttc
    55381 tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct ggcagtcaaa
    55441 tcggctatta caaaacaaag ataaggtaat tcagtgaaga aaatcatttg tctggccatt
    55501 acgctgctaa tgacactccc cgcgtacgcg aagttaactg ttcatgaaga agcccgcatc
    55561 aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg caacggtgat
    55621 gaacatacct gcgatgaagc ggtttcccac ctgcgtctga agctcggcaa tacccgtaac
    55681 cgcattgaca ctgccgagca gtttattgat aaggtagctt cgtcgtcatc gattaccggg
    55741 aagccgtata tcgtgaagat ccccggtaag agcgatgaga acgcacagcc ttttttacat
    55801 gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc cgctttggta
    55861 aggcatttac aggaggtaac atgaaaaaat gctttattta tcacgatgaa aaatcgaata
    55921 aattttggtg gatagattac gaaggggata gtttagctgt caactatggc aaggtaggta
    55981 gtattggtaa attccataca aaagaattcg ataatgaaga acagtgtctg aaagaagcca
    56041 gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga aactccagac tttaacttca
    56101 tggatctcta ctattttgat gatgaagaga ttgggttaca tcttaaaacg tcacacccaa
    56161 acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa gaatctcctt
    56221 ttggtagcga tgaaggtgct gatgctctaa acgaacttga aaatagcctc cgttctggac
    56281 tgtgctgatt tccctcaaat gttaattgaa actatgtggg gtatgaaata catcgctatg
    56341 gacagtattc ttgaagagga tgtgcgtgcg caattactag ccgatgaaat gagctctatc
    56401 cagagcaata tgattaccta cgcgactgca ttcggtcaga tcaaagtcat gggtaaaatc
    56461 tcccataaac ttaaaaagat gggactcaat gcactagcgc gtcatcagct taccgcaaaa
    56521 attcttcaat ggggtgacgg tcaggactca ccaatacttc aaaaaatgat tgatgacctt
    56581 actgcttttc cacacgaaaa ttaaaaactg catttgtcgg cagcaacaac tgccgacaaa
    56641 tcagattatt gacaaagtgc actttgttta tgccggatgc ggcgtaaacg cctgatccgg
    56701 cataaaaata ttgctaattc aatatattgc agaaacattg taggcctgat aagcggagcg
    56761 catcaggcag ttttgcgttt gtcatcagtc tccaatgcta ttaatcctta aatccctgcc
    56821 cccagactaa aatgctcttc cccaaacact ccggtggaga ggtagcgatc gccacgatcg
    56881 cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag tgctccagca
    56941 accgcgccgc ctgagctgac gccacagaat attccttccc gcaccgccag ttcgcgcatg
    57001 gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagcgc agcgttgaaa
    57061 atccccggca gatattccgc aggccagcgg cgaatgcccg gaatactgct gccttcttcc
    57121 ggttgcaggc caacaatggt caccggtttg gattgttcgc gcataaagca tgaaacgccg
    57181 gtgatagtgc cggtcgtccc catgctggag acaaaatgag tgatgcgccc gccggtttgc
    57241 tgccagattt ccggcccggt ggtggtgtaa tgcgcataag ggttatcggg attattgaac
    57301 tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc gcgcgcacct
    57361 tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg catcgccgca
    57421 cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc tttcagcgcg
    57481 gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa gacatcaccc
    57541 ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc acgatctttc
    57601 accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc gttatccggc
    57661 cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa tgtactcaca
    57721 atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag
    57781 gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg cgtttaacat
    57841 tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatcgcggg tttcaatacg
    57901 ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct cgccatgctg
    57961 cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg
    58021 caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta ccggcagcgg
    58081 cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga agagatccac
    58141 cggcccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa catggaactg
    58201 cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga gtacaaaacg
    58261 ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat tcccctgact
    58321 catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg tcacaaacac
    58381 gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct ctttacgcac
    58441 ctgcgcatcc agcgcgccga acggttcatc aagcagcaga atttgcggtt ctacagccag
    58501 cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa agctgcgccg gataacgatc
    58561 cgccagatgc gcaagctgga ccatttccag caattttgtc actttcgctt tgatggctgc
    58621 ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagtgatat tgtcgaatac
    58681 cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac gatcgcgcgc
    58741 gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt gatgctccag
    58801 cccggcgata atccgcagca gcgtggtttt cccggaaccg gacggcccca gcaacgcgac
    58861 catctgacca gaaggaatat ccagtgagat atcgttcagc acctgggtgc gaccaaaaga
    58921 cttcttgata ttggcaatct caatgctcat gatgctcctc ctgctgcgcg cgtttttcct
    58981 gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc gccatcaacg
    59041 tcaacagcgc ggcggcggta aaggagccga cggtgttgta gtcctgctcc aacaattcaa
    59101 tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc ggaaaccacc gacactgcac
    59161 caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc gtaaagcagc gcccagcgaa
    59221 tattcggcaa tgtgacgcga cggaacatct gccagccgga cgcgccaagc aaaatcgccg
    59281 cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc accacaaacg
    59341 gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata atttgcagat
    59401 tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc agatacacca
    59461 gaccggcaac caccggcgat acagcaaacg gaatgtccag tagcgtcagc agtaactggc
    59521 gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac accaggttta
    59581 ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc atgtccggat
    59641 cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg aagatgtaaa
    59701 tcatcggcac cagcaggatg aacgccgaaa ccagcatccc gatgccaatc agaaaccatt
    59761 tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc gccattaatg
    59821 acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca gcagcagcag
    59881 agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc gggtaatcaa actcctgtaa
    59941 gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac gcgatatttc cggcgataaa
    60001 aatcaccgcg ccaaattcac caagactacg ggtaaacgac agcgtcacgc ccgccaccag
    60061 cgccggagaa agctccggca gcaccacttt gcagaaactc tgccagcgcg ttgcaccaag
    60121 cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct gcacggtacg
    60181 caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc aggtataggt
    60241 gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg aaaagagcga
    60301 ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc
    60361 aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca gcagaccgaa
    60421 aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt aggccgcgac
    60481 cacctgcggg ttggtgatca cctcccagta ctgcgcccag ctcatctcag aaagctgcat
    60541 caccagcgca gagagcggca gcagcaatat caggcacaca aacagcagac tggtgccaag
    60601 gcttaaagta aagcccggca gcacgcgtct tgaagaaact gcaaacatta cttacgcccc
    60661 gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat cacttccggc
    60721 catgagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt atctttcagt
    60781 ttgtccatga cttccgggtt attcacacgg tagtaatagt cggtgatgat ggtctgcgcc
    60841 tgtgggctgt agagccagtt caggtaggct tttgcggctt tttccgtacc gttggcctgc
    60901 acatttttat cgacccacgc caccgggaat tccgccagaa tattggtttt cgggatcacc
    60961 acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga ttcaaagcta
    61021 atcagcacat cgcccaagcc acgctcggca aaagtggtgg ttgcgccacg accgccggta
    61081 tcgaacactt caacgttttt caggaactgg gtcataaact gttcggtttt ggctttatcg
    61141 ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg cgcgttaccc
    61201 gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc gttccagtcg
    61261 tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta gaacggcgag
    61321 ctattattcg gcaggcgcga ctgccagtca gccgggatca gcttgccttt gtcgtgcagg
    61381 atttgtacgt cggtcacctg gttataagtg acaacgtctg cttttaagcc ctgcaaaatc
    61441 gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt gtcgccgccg
    61501 ttatcttttg cccattgctg ttcaaacggc ggattcaggg cggcaaacag ctcgcgggag
    61561 acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag cagcagagaa
    61621 gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca cccttataaa
    61681 tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt aacggtttta
    61741 tataccgttt ggtgatttgg aagttaaaaa gggaataaga acttggttca acaggcgtaa
    61801 ttgttgcagg cagtttggac acggacagcg cggagaaacc ggagcgtaca catagtacgt
    61861 gaggatttcg agcactgccc ggggccaaaa tgacaaatga aatagcctgg tgaacttagt
    61921 tcaagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg
    61981 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    62041 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    62101 cgacacagcg ggattgcttt tgccatttca gtcagcaccg attctggatc ttccgggttc
    62161 gactggcggg caatgctttc cgccattggc gtgcggacgt atcccgggca gatggcgtta
    62221 acgcgaatac cggattgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    62281 gctttcgtta aggcatacgc cgtttcgcca ggatcggcca ccatatcacc agtgactgaa
    62341 gacatcatca caatgcgacc atctttgcgc gcaatcatct ccggcagcac cgccttcgtg
    62401 acgttccata cgcctttaat attgatatca atatggaaat cgcgatcttc atcgctcata
    62461 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    62521 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    62581 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgctagctt ttcgatctca
    62641 ggggagatat ccagcaagat taggttcgcg ccatgacgtg caaaagttct ggcaattcct
    62701 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    62761 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    62821 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    62881 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgacg
    62941 ccacatcatt gatgttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc
    63001 gtgcgggtag acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg
    63061 aaatgcgcca gccgccgcag cggagagtca gccagagaag taatggcaat caccgttgcc
    63121 ccctgctttc gtgccgcttc tgcgcacaag acaatctctt ttttactgcc gctgtaagag
    63181 atagcgattt gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg
    63241 tcggcttcgc aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc
    63301 accagcgccg atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca
    63361 atgatttttt gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct
    63421 ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg
    63481 tgcaggtgca gcgccgtggc attcgttttc tcccggctgg cactgtattc gccaattaac
    63541 gccatacgca attcagtaaa tccttgcgcg ccgagcttct gggcaaattt cacaatgctc
    63601 gactggctga taccgagctg tttcgccatc tgccgtgaag agacggattt cagctcgctc
    63661 actctggccc gcagaaagtc agcaattttt tgctcgttcc cggtaaattc actttccgca
    63721 ttgcgtattt tcgtcaggta caacatcttc cactctcctg ggctggcagc aaaaattccg
    63781 cttaagttct tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt
    63841 ctgtcaattc gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg
    63901 tcaccaccga tgcaactaga aaagatgatt actgaaggct cgaacaccgc ctcggctgaa
    63961 attgaccgcg tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta
    64021 ccgcctgccg ttgatcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc
    64081 caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg
    64141 attctggatg ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt
    64201 ttgattgctg gaggcgaata tgccattcag cacgcggtgg aaggagctga agatagccgg
    64261 gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc
    64321 attgctgcca gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc
    64381 ggctgccgca cagtgggaat ttcctgtaat ccggggagcg ccgtttcaac caccgctgag
    64441 tttgccatta caccgattgt aggtgccgaa gttgttaccg gttcttcgcg gatgaaagca
    64501 ggtacagcgc agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatccggc
    64561 aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc
    64621 caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta
    64681 attgcctgcg agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc
    64741 gcagaagcta aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag
    64801 gaataaccca tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc
    64861 ggcggaccag gaaatatcgc cagttgtggt aactgtatga cgcgcctgtg tctgggtgta
    64921 catgacagtt cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt
    64981 ttgaccagcg accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa
    65041 gcgatgagcg agctgctggg cgatgcaccg gtacaggatg ccgcagaaat cgccgcccag
    65101 aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc
    65161 gccaccatct tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata
    65221 gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac
    65281 gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca ccttcctagt gattctggtg
    65341 ggctataacg ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca
    65401 ctctttttgc tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat
    65461 ttctttggtc tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg
    65521 gcctgcgccc gcatttaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg
    65581 ctgacctcgt taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg
    65641 ctgggtggct ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg
    65701 ttcggttgtg cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag
    65761 ggctttattc ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc
    65821 atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg
    65881 caaccgcaca gtgcgctacg cagtcaggta cgcggggcga ttattcccgg cctgctgggc
    65941 gttggtgaac cgctgattta cggtgtcacc ctgccccgca tgaagccgtt tgttaccgcc
    66001 tgtttaggcg gcgcggcggg cggtttgttt atcggattga tagcctggtg gggtctgccg
    66061 atgggcttaa acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc
    66121 gcacaaggca tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt
    66181 tgcgggttta ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatattat
    66241 gaaacggaca atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa
    66301 ataccctgtt ctgacagaag gtagttgatc aagtatatgc ggcgttaaag cagtaataaa
    66361 aaggccagtc agtaatgtta ctgcctggcc tttaatcaat gaatcagaaa cgcttacagc
    66421 gccatcaact tgtccagcga cggtgcgaaa taatagccgc cggttaccgg tttggtgaaa
    66481 cgcaacatcg catcacgttt gccatcggta tcgccaaaca tgctcagcag ttgctgctca
    66541 atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc actggcggtg
    66601 ccgtacggca ggctctggcg aacaatcttc agccctttgc catcttcttt cagatcaacg
    66661 cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg
    66721 gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc ggttgagctg cttcaggttg
    66781 tgctcccaac gctgcacaaa cacatagctg ccgccggaat ccacgccgtc tttgataacc
    66841 gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac aaagccgctc
    66901 aggtcacgct cttccaccca acggaagccg tggatctctt ctttcacttc aatgcagtca
    66961 ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta cgtcgtgacg cagagaaaga
    67021 atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc ctttaccgta gcccggaaaa
    67081 tctttcagct cttctgcccc aacgccgccg ctcagagcgc gccaggtgtt gttaccaaag
    67141 gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg
    67201 gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc
    67261 caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga cattgttcct
    67321 cctgaaaata agaatgccac gcaatccgtg gcattgttga gcgctatttt acgtgttttt
    67381 tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca gataatttta ctgactttcc
    67441 agtttttcag ggtatcgtcc gaaggcatta acccttcagg accacgccat tccccgctga
    67501 actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga ttatcatcac
    67561 catcagcttt ttggcaattg ccaaatgctt tgctgtaaag gtcgctaaac ggtgtaccga
    67621 ttttaacacc ggtgtcagca ggaatatcgc tatccagcac gtcaatgcga ctgatcgtgc
    67681 cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt catcacttca aaaaagcgca
    67741 ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat
    67801 cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc
    67861 tcactttggt ggacgatcca aaccagttcc acgggttagc ggcagaccag ttaactgacg
    67921 acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa cgcagcgatt
    67981 tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc accaaaaagt
    68041 gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga
    68101 ttaacgccac aatatccgcc actaccagcg ccagaccgat accgctaacg gattcaccgt
    68161 tcagccacag ccacggttgc cagcaaagta aaaccacctg cgccaacagc aacagaaaat
    68221 agagcacatg ccaggtgcga ggaaacgaag cccgccgacc gctcaacaaa aacgccagca
    68281 ccgccggaat accgggaatc agccccagcc agaaattatc gtgatcggga taaaacagat
    68341 ttagcagcgc agtaccctgc tcacgcgacg caccggcaat gacaaacagc acccaggttc
    68401 gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcgt ccgtgcgcat
    68461 cataatggac aggatggaac tcagtactct tcatcttcaa tcaaacgctt acccagactc
    68521 agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat gtcgttatct
    68581 tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa tcagcttttt ctccagccgg
    68641 ttaagcaacg cattggcaat tccacgccca cgaaactctg gatgcacgcc aagataataa
    68701 gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc accgtttacc
    68761 tcagcgacca aaaacaaact gacgtcatgg ttcatcttac gctcgatgtc catttccgga
    68821 tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct
    68881 tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt taaaaggctg
    68941 attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga aaaagtggca
    69001 taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc
    69061 gtaacggatt acgcgatacg atataacatc tggaacttta ttattacaac tcaggccgta
    69121 tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg aaagccggtt
    69181 tagctgccct gacgttgtca ggaatgtcgc aagccattgc caaagacgaa cctttaaaaa
    69241 ccagcaacgg acacagcaag ccgaaagcca aaaaatctgg cggcaaacgt gtcgttgttc
    69301 tcgatccagg ccacggcgga attgataccg gagcgatcgg acgcaacggt tcgaaagaaa
    69361 aacatgtggt gctggcgatt gctaaaaacg tccgttccat tttgcgtaat catgggattg
    69421 atgcgcgttt aacgcgttct ggcgatacgt ttatcccact ttacgatcgc gttgaaatcg
    69481 cccataaaca tggcgcagat ctgtttatgt caattcatgc cgatggcttt accaacccga
    69541 aagctgccgg tgcttcggta tttgccctct ctaaccgtgg ggcaagtagc gcaatggcga
    69601 aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaag gcgactgaca
    69661 aggatcacct attgcagcaa gtactgtttg atctggtgca aaccgatact attaaaaaca
    69721 gcctgacgct tggctcgcat attctgaaaa agattaagcc ggtgcataaa ctgcacagcc
    69781 gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttcct tcggtgctgg
    69841 tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg gcggcgtttc
    69901 gtcagaaaat cgccacagcg attgctgaag gcgtgatcag ttatttccac tggttcgaca
    69961 accagaaagc acattcgaaa aagcgataag ttatgaaacc cgacgcacac caggttaaac
    70021 agtttctgct caaccttcag gatacgattt gtcagcagct gaccgccgtc gatggcgcag
    70081 aatttgtcga agatagttgg cagcgcgaag ctggcggcgg cgggcgcagt cgggtgttgc
    70141 gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc tcacgtccac ggtgaggcga
    70201 tgcctgcttc cgccaccgct catcgcccgg aacttgccgg gcgcagtttc gaggcgatgg
    70261 gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc caccagccac gcgaatgtgc
    70321 gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg gtggtttggc ggcggcttcg
    70381 atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc accgcccgtg
    70441 acctgtgcca gccatttggt gaagacgttt atccccgtta caaaaagtgg tgcgacgatt
    70501 acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc tttgatgatc
    70561 tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc aaaggctaca
    70621 ccgacgctta tttaccaatt gtagagcgac gtaaagcgat ggcctacggc gagcgcgagc
    70681 gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg
    70741 gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg tcaatgccgc
    70801 cactggtacg ctgggagtat gattatcagc caaaagatgg cagcccagaa gcggcgttaa
    70861 gtgagtttat taaggtcagg gattgggtgt aactccctca cccccattcc cgcatccgct
    70921 gatgcaacgt caacgacggc ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc
    70981 ccagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg
    71041 ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg tttcagccac gcgtttgggc
    71101 caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg gcgactgaca tgcagttgat
    71161 tacacaaatc cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc
    71221 gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc attggttgcg
    71281 cttcttccag catcgccccc atcgccatta gcaaattatc ccccagcact tttcgcactg
    71341 caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc
    71401 cccacagcgc ggctttatgc tgctctttca cttccagcgc cgactggcta cgcagcatat
    71461 gtaatacccg atccggattg tgcaaaaagt tagcctgaca ggtgatgaca tcttcagaca
    71521 gcactacgcc caggatcgta taatcgtccg gcgtacttaa ttcaaactcg gttccaccgg
    71581 ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc gataaatccc tgctcaccgc
    71641 gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac tgacgcagcg
    71701 ccagaccggt gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac
    71761 catgaaactt gcccggatgc agctgatcgt aaatctgctg ctaggcggta atcgtcaaag
    71821 catgttcata gacatccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg
    71881 tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg
    71941 tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat
    72001 ggggcttgag acgataacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt
    72061 cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcggccact tctcctgccg
    72121 tcattccctg acatactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga
    72181 taactggcgc gtcgggttcc tttacgggtt gcttcggctg gcggttaaag ccagtaacca
    72241 gccactgggt gtcatcgtct ggccgaccga tctccttacg gctgatgaca cgcccggtac
    72301 gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca
    72361 ccaccagcgt taagcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg
    72421 ctttgagcat ggcatccgct gcatctatcg cggcgaccat gccgtccact tccagcaatc
    72481 ccagtgcatt gatcatcggc aacctccgtt acgcacgctg gattggatta cgcgcgattt
    72541 ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg ctaccggtta
    72601 aaaatgctgc cgagtagttg gtttcagacg gtggcgggac gtaggtcacc agctgtacgt
    72661 cggcagattt caacgcggca tcaatgccgt aggtcgcttc cagcggcggt gccaccagat
    72721 acgccatcgg atcgccaagc gtgatcccgg cggttgatga aagataagaa ccggtacgcg
    72781 aaaccacatg cgccaggaag gcggtgtttt ctgcgtcatt ggcccactgg aaagctgcgc
    72841 cattttcaat atgtgcaacc atcgcatcca gaccggcacg cacttccgcc gggtttggcc
    72901 caccaagcat aatcagcact tcaccggcag tcggtgacgg gccgtgtgcc gctcccgcat
    72961 acagcgagcg accatatacc acttcgacca tcgcttgttt ggtcgcttcg tcagccgcaa
    73021 tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag gctacgaata tgcggcggca
    73081 attttagttc acgcgcaaat tcagcgttaa cggaggcaat cacccgcatg gcggttaccg
    73141 acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgctgg
    73201 atgctttctg ctccagcata cgtttcgcca aatccacaat gacggcacag cagcacgaaa
    73261 gagaagcaaa agtcagctac ctggtacact aggcagcggg tcagcaaagg ggggcaaatg
    73321 cccctttttt catgtcagat aatcagatca ttacctcaat aaactaataa tcacatcgcg
    73381 gttacaacca gtttgatagt gatagctcaa acccagacgt ggcgctggct gcaagcaaaa
    73441 taagccgcaa aaatgcgacc ccatgtttac tagccgcaaa aatgcggtcc tatttgccag
    73501 aaccttttat tattcaattg gttagtgcaa gaaaaagcgt gattgctgtt caagaagtat
    73561 ccagaaaggt catgattttg acgctgaaaa aaaataggtg ggcatttttg cccactcact
    73621 agccgcaaat ttgcccactc actagccgca tttttgcggc ccaaagtaat aaatacagag
    73681 aaataataaa tacagatcag cgccaagatt tttcgtcttt tcggcggaat catgagacta
    73741 gtgcttacgt cctgataacc tccagagcag accttaactt ctgtgcagca ggactacgct
    73801 cattgccgct cggctaggtc aaatcctcac gataaagctg ttcggattgt gaccataatg
    73861 ttaaaggact ctttaaatca ttccttgacg aattaactag ctggcttaag cggaatagca
    73921 tgcaggataa aaacaaaacc agcttagcag tggggagagc ttcgcttcgc tcgtctcctg
    73981 gggccaatgc tcaatgaaac gacgtaaggc acggagcgct caccataggt agacacataa
    74041 acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat aaaaaccaac gacaccgcca
    74101 cactgcaaga ccaatggttt ttatcgcgta tacagaccag acaggaaaca gcagcaaagc
    74161 agcgtatagc caggtgcaga cagcacactc acacgacgca cagcaggctt aagtgatcac
    74221 tcaggaaact ttatccccgc cgccgatcac cgcgaactat catgaccgcg ccaaattcga
    74281 aaggaatggc ccatagaacg cgatgaacac acataagcgc tacacttact taacgtcgcc
    74341 acagagcgat tgagagcgct taaaatcgat gcgatttcat taaatttttc tcactcgcct
    74401 gaatggtggc gatcaggact gatcgcaaca ttgccccgcc tcgatattga gcgcataata
    74461 gatacataca ttaagcggaa aagagataag tttattgtga aaatcagggg gttacatatc
    74521 atgcgcatat cagaacaaca acggcagatc ctcgactatc tggcaaaaaa cgaatcaatc
    74581 accgcacgac aagccgcaga tctcgtttat ggtggcaatg ttacccgcgt ccaggttgac
    74641 gccgcccgcc gctcactcat gacgatggtt agtaatgggc taatcaggaa acaaggccgc
    74701 gctttcgtag ctgaaagaca tatcaatcac gcttatagcc aggacgttga agaattgcgc
    74761 cgccagatcc tttgggtatt cagcaaacag ggtcaggagt atttcctttc tgaacaccca
    74821 aatttcgccg ccaaagagtt aacagtaagc ggattgtcgt gcgcactgga ttatcttgtc
    74881 ggtgaaggtt gcgttactta caagcagcta tggcacgccc tgaatacgct tgtagaagat
    74941 ggtttactca ttgccacgcg tgagaacatc gaatactgga taatgccgcg ttcaggtcgc
    75001 cgcaatcatg cacggtatat ttcaggcaac agatacagaa cgccggactt taatccgaca
    75061 caggaagatc gggaccgtgc cgaaatgatg tgcagggaac aggagaaaag aattatggat
    75121 aagttatttt cgatgcagtg aacacaagcg ggcgcaatgc ccgtttttat tgccttattc
    75181 cacgcctgac gaatcaattt gatcacacct gagaaaatgc atagccagaa gttaaggccg
    75241 ttatattgcg attgggagcg ttttaaacgg tatcaattta attaattctg atgtggtgat
    75301 ttgcaggcaa aaaaaacccg ccggagaagc gggaaataga gacatatcgt tgtagttatt
    75361 aacagatcac tttgaggtcg agggcggcta accctcgcta atattatccc gctattacct
    75421 tgcgggtgca aataatcttt tattgctttg atgatttaat aaatagcact ttttgctaac
    75481 aaaaactgct gtcaatatat ggccaaaaat gtcaacagaa aaaataaggt ttataatatt
    75541 aagtatcaca tgggtaatgg gttcagggaa tcggaatcca ccgcctcacg gaaattcaaa
    75601 atttgcttct tctttcctcc gtgggcggct tctttttcgt atcgaggtat ttatgaaagc
    75661 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    75721 gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta ctcgccaatt
    75781 attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat aatcacgcat
    75841 cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt gccgatatta
    75901 tgcggcgcag atggatttat gaccctgaag cgggtttatt gtttagccgt gaaaaaaaag
    75961 acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta catgaaggta
    76021 aattcaaagt gacactcacc tatcagaaag cctgctatgt atacacatac ggcgcttatg
    76081 acgagacatt atatgaagtg caccacgtta atttgaacaa acaggataac cgcatcaaga
    76141 atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc cgcaaactta
    76201 ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca ctataaacac
    76261 aggagtaaat accatgcatt cattagacag ttattttcag cgcacgacag cccctaaatc
    76321 agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg ctgattatat
    76381 tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg ataaacttca
    76441 gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt gcgaattaag
    76501 tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg ggaaagataa
    76561 tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag cgtgataaaa
    76621 ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc cgtatggccc
    76681 agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc ccacaaatcg
    76741 ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta aaaacgaacg
    76801 cagagaaaat ggagatgcac aaacagagcg gtgctccgaa tcccgatgag tacttctcac
    76861 cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc accgaggaac
    76921 agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac tcgtatttcg
    76981 gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg
    77041 ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg attcggtact
    77101 gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga ccaatgaaca
    77161 aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac tatattagca
    77221 caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc gctaagaccc
    77281 tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc atgaagcgcc
    77341 aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc atcgacatga
    77401 aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt ttaattctca
    77461 ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat gatgggacgg
    77521 gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga agcaagatat
    77581 tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca
    77641 ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca tcagtttcac
    77701 caattactgc atctacgagt gttacaagga agatgcagag gactggctgg attcattgcc
    77761 agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc atatcacctc
    77821 tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc acaataacga
    77881 tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat tgaaacacat
    77941 ctaccatgac cataataaag taattttttg atatgtttat ggttttatat taattaaaat
    78001 atatctaaaa ttgatagttt taaaattgca aaatccagat tgtgtttgta atgtttaatc
    78061 tgattgtatt gagtggcgtt tattatgaaa aataaatcag gtaattcttg ttatagagaa
    78121 gcagtcgata ttcatagctc acttgagctt tcatttatat tgcataaata tcatttgcgt
    78181 gtagatgaga agaaatctcg aaatgtattc agcgccatac gtgatgagct catccttgat
    78241 ggggattcac gacaaaactt agctactttc tgtcaaacat ggcttgatga tgaaatccgt
    78301 gagttgctta atttagctat tgataaaaac atgatttata aagatgaata tccacaaatg
    78361 gcggaacctg aaggtcgctg tgtgcgtatg ctttccgatt cgtggagttt ccccgattcg
    78421 cgacacacct taggttgctc aaccattggt tcatctgaag ctgctatgct cggccgcttg
    78481 gcgttaaaat ggcagtggtg taaaaaacgc gaagtacaag gtaaatcaac cgaacctgat
    78541 ttgcgatcct gtgcaaatat gctggcataa attctgccgt tactgtgatg tgaaattgtg
    78601 tgaaattcca cttgatgatc atcacctgat aatgaatgct aaagaggttt tgaagcatgt
    78661 cgatgaaaac actattggtg tcgttacgac tttaggtatt acttattttt gtaaagcaga
    78721 ttgcgttagt gtttttaaga taagtgttaa agttaatatt agtttttata tttacatcgt
    78781 aattatatgt ttctatttta aaaatgttcg agagtataaa aatgtctaat gctaatatcg
    78841 cacccaaaag taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag
    78901 taagtctacg atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata
    78961 cctttgctgc aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa
    79021 catatccaca aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg
    79081 gttttgtagg aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga
    79141 catttggtgc agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg
    79201 ccagtaataa atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg
    79261 ttgcattacg tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga
    79321 caattattcc agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta
    79381 aaccagcggt gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga
    79441 atattgttct tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc
    79501 atgttaacga tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta
    79561 taggtactat tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag
    79621 ataaaattag tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg
    79681 gagtgccatg gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg
    79741 tggttacttg gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt
    79801 tacctaagtt tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac
    79861 aaggtataat tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag
    79921 cttttcagat tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt
    79981 tcgctagtgc tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc
    80041 ctgctttatt tttctgggca acacttggat tcttagggtc ttttctggct tttgtattaa
    80101 gttttattcc gcccgagcaa atcccaactg gtagtccaac cagctatgtt ctttatttgt
    80161 tagtattagt tgccctattt ttattaattc caatattaat ttacgccttc cgaaaatctg
    80221 actggaaaga tccaaactct gatttcgaac cattttcttg ggaaaaaaga actagtaatt
    80281 aaattttcca taagctacaa aagatgaggt taaatatgtt tagtgctgaa tctccaattg
    80341 gtgtaataga ctcaggtgta ggtggattga catcggtaaa ggaaataatt aatttgcttc
    80401 ctggtgaaga tattatctat tgtggagata atatgaatgc accttatggc aaccgttctg
    80461 ccgatgatat catcagtttg actaaaaaaa tgctgacttt tttacagtca aggaatgtca
    80521 aactagttgc tgtcgcatgt aatactatat cctcaacttt ggaatccgaa gaatacagcg
    80581 gatatgctaa gagctttccg tttcctatct tatccatcat tgaacctgca gtagaagatg
    80641 tgattcgcca gcaatataaa aatgttggta ttatcgctac cgagtttact attaaaacgg
    80701 ggtgtcacaa agagctaata aaaaaattga attcaacaat aaatgtattt ggagaaccaa
    80761 gcaaaaatct tgcaatgtta attgaggaag gtaatcttaa tgctcctgca atcttgaatg
    80821 acatcaaaaa acatgttaac catttaattt ccctccatcc agtaaatgaa attattctcg
    80881 gctgtactca ctatccaatt gttcagaatt tttttgaagc agttgcaccg gatataaaat
    80941 ttattaatcc tgcacatgat caggctatct ctattaaaaa tcacttggga cagctaaatt
    81001 tattaaactc ctcaaatata ggaactttgc aaataaatac tagtggaagt atggagatct
    81061 acaaaacggt actaggggaa ttgagcatta cgaaacctca tacattcagt atcaggcagt
    81121 tttaaattta tccggtgcac cgtaaaatcc cgtccttctg aactgcaccc caaaagttgg
    81181 acaccaactg agtaaggtgc attttatgga caaacccagt tacatactgg gtagtgcatc
    81241 caattagtag aacatgtgtt tttcgataaa cgctccgatc actttttcgt ggatctcaac
    81301 tgagcgtgag aagcagattg ttttacgacc caaccgctta atgcgggtgc gtagcgtcag
    81361 attattacgc tcaatgcgtt gggtgaatat tttgccggtc agatgcttat tcttcggcac
    81421 ctcccggcca tagctgcccc agtcatcgct ggtcagcatg ccgatgttga agggtgtaag
    81481 cagtgccagt agctcccggc acgtttgatc ggttcgggga ccaaaagtgt aggccagtac
    81541 accgcctgtt ttggtgttgt acgcgtaccc gagccaatgt tgccgggctt tactgccaac
    81601 gtagctccat tgctcatcaa gctcgcagat aaacgccaca tcagcatggg caacaggcga
    81661 agaagttatt cgctttggcg cgagtttttt aaagtccgga tgacggtgtt actaccaatt
    81721 ttcagtgtcc tggcggtatc gcgaaccccg gcaccattaa aggccatttc agttatcagc
    81781 tctttcatac ccggcttacg tgcttgataa gtataagtga actgaaacac acggtggcag
    81841 tcacggcagc gaagtctgtc acggccttta gggttctgac catggcggta aacctgagct
    81901 gactgacaac ggggacaatg aatattaacg ctggtcatga gataacctca aaagcccgta
    81961 ttatacatca gattcaacta attagaggca tcacccatcc agcattataa aacatactaa
    82021 caataactaa attataataa tcacaactta caatcaaata ataaatgcat aagattaaca
    82081 atgatttttt cgggttatat ttatctaaaa aagcagaata ttatgtaact tattattttc
    82141 taagcgaaaa tttattgtgc ggtcagacta acaaacttgc gtaccacccg aagaacatgc
    82201 gaagatttaa gtattctttc ttttttggtt tttatttttc attttctctt ttcttctctt
    82261 tccggttctg ttctggtttt ggttttttcc tttttatttt tattctttct tcagggttat
    82321 gctaaaacac caaaacattt ttcaccacaa aagcatccaa tcatcttgcc agaccaatca
    82381 ccttaaccgg atacactgcc ggatataatc ccactaacac ccagacacca agcaccatac
    82441 ctgataacta ccgtattcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg
    82501 atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt
    82561 caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc agagctttac
    82621 caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat
    82681 cgaccagcgc gttgatgacc gtcagaaccg cggcgtaacg cacaggcagc gaggcgggaa
    82741 gcgccagcac gtacacgtca gttttgggat caagatccag ctgaagcagc gcctgttgcc
    82801 aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca
    82861 gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt gcacccgcgc
    82921 caatcaccgt ggagcgtacg gtttgcgccg gaaactgcac attcatctca cgcaggcgcg
    82981 gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag aacgggttgg
    83041 cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa
    83101 cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt
    83161 caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg
    83221 tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc tgccccggtt
    83281 tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca ccgacgttga
    83341 gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc
    83401 cgatatcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccggctc
    83461 cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca
    83521 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    83581 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    83641 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    83701 taaaacccag tagatccgcc acttcacggg cttccggggt aataatgctg gcgcgcagaa
    83761 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    83821 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    83881 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    83941 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    84001 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    84061 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    84121 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    84181 tccacggcca gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    84241 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    84301 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    84361 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    84421 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccggc gatcatcgcc
    84481 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    84541 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    84601 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    84661 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    84721 aggtagatca ataagaatgg agagatattt actgttatga ataaactttc atatgcgacg
    84781 catgaatata aataaaataa tatcgagata ttttatatac tgattttttt attattcgca
    84841 aactcagtaa taagctttgc taatatattt caggaaaacc gcttaccctg aatattcagg
    84901 gtaagcgtga gagttaagaa tgaattacag cggttgggtt tgcgcttcaa ccacggccag
    84961 cgccaccata ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    85021 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    85081 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    85141 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg
    85201 catttcacca tcaatcatca gatctggcgc acgatccctg accagttcca gcgcctgacg
    85261 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    85321 gcgcggctca ataccaaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    85381 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    85441 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    85501 tttcaccacg ctaaaatgtt catgataatc gcccaccgta ccgcagatca ttgcatcggc
    85561 ttccccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcacgctg
    85621 cgcctgttcc tgagtgacac cgcgacgctt catgatctgg aagtattcgg tccagtactc
    85681 tttaaagcgc ggatcggatt cgttattgac gatttcaaaa tcaacgcccg ctttgatctg
    85741 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    85801 cgccagtccc agcgttacca gttcctgagt cgcatgcaga acgcgcgcct cttccccttc
    85861 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    85921 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    85981 acgcgtcgcc acgcccgact ccatcgccgc ttttgcgacc gcaggagcga tcttaacgat
    86041 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgaac
    86101 gccatacgct gaagccacca cttcactctg ttctgcatgg gccagttctg caatcgcgcg
    86161 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    86221 gaagatgaac gggaagcaaa gaacgttgtt cacctggttc gggtagtcgg aacggccggt
    86281 acagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    86341 gttcgccagc gccaggatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    86401 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    86461 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatat ttggctcacg
    86521 gccctgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagccccag
    86581 cgctaccagc aggttcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    86641 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    86701 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    86761 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgatgc cgccgaaggt
    86821 cggttcgagc gcggcgacga cttcaataaa tttgtccggg tcgagttcat caacttcaat
    86881 gtcaaataca tcaatcccag cgaatttctt aaatagaacg cccttgcctt ccatcaccgg
    86941 tttgcctgcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    87001 cgcaaccagg ttaccgcgcg cggtatattt gtaggctttt agcgggtctt tttcgatttc
    87061 aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct gcgttgccag
    87121 aggcttggtt ggagaaacct agattttccc tggaactgga aattcatgga aatcaagtgc
    87181 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    87241 tgtgtgggca acagcccctg cccacaacgt ggcgcacatt attaccctgc cggagtctac
    87301 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    87361 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    87421 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    87481 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    87541 agctgcgata ttgagtccat tcgccattat catccccagg atgccaccac taatccttcg
    87601 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    87661 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    87721 gcggtcaatt tcggtgctga aattctcaaa atcgtacccg gttgcgtgtc aacagaagtt
    87781 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    87841 ttgtatcagc aacaaggcgt tgagaaatca cgcattttga tcaaactggc ttcgacctgg
    87901 gaaggaattc gcgcggccga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    87961 ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg tagggcgtat
    88021 ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag aagatccggg
    88081 cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg aaaccattgt
    88141 gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct gcgatcgact
    88201 gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttctccag tggtacgtaa
    88261 attaatcccg ccttctcaga cgttcccacg cccagcaccc atgagcgaag cggagttccg
    88321 ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca ttcgtctgtt
    88381 cgctgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa ccagccacgg
    88441 agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt atggatgcgg
    88501 tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat attgccgaag
    88561 tgctgtggaa cgattttctt aaacataatc ctaccgaccc aacctggtat gatcgcgacc
    88621 gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgcta catctgaccg
    88681 gttacgacct gccgctggaa gaactgaaga acttccgtca gttgcattcg aaaaccccag
    88741 gccacccgga gattggctat acgccaggcg ttgaaaccac caccggcccg cttggacaag
    88801 gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac actggcggcg cagtttaacc
    88861 agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac ggctgcctga
    88921 tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggacta ggcaagctga
    88981 ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc tggtttaccg
    89041 acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa atcgacggtc
    89101 acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa gataagccgt
    89161 cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca ggtaaggaag
    89221 aggcgcacgg cgcaccactg ggggaagaag aagtggcgct ggcacggcaa aaactgggct
    89281 ggcaccatcc gccatttgag atccctaaag agatttatca cgcctgggat gcccgtgaaa
    89341 aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa aaggctcatc
    89401 cgcaactggc agaagagttt ccccgacgga tgagcggtgg tttaccgaag gactgggaga
    89461 aaacgactca gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc gctacccgta
    89521 aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg ctcggcggtt
    89581 cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg ctgaaggaag
    89641 atccggcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc gctatcgcca
    89701 acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg atgtttgttg
    89761 aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag attatggttt
    89821 atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct gttgagcaac
    89881 tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat cagatggaag
    89941 cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca ctgatcctct
    90001 caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag attgctcgtg
    90061 gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt gccaccggtt
    90121 cagagatgga aattaccctg caagcggcag agaaattagc aggagaaggt cgcaatgtac
    90181 gcgtagtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa tatcgggagt
    90241 cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt gccgattact
    90301 ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttac ggggaatctg
    90361 ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt gtggcaaaag
    90421 cgcataaggt gctgggagtg aaaggtgcct gatggtgatc gccggatgct gtatgttccc
    90481 ctcaccctaa ccctctcccc agaagggcga ggggacggtc cgtgcgcttt ttagggccgc
    90541 atctgcgatt ttgtttaacg ggtgatccac aacgtgggcc tggcgtctgg cccatgccag
    90601 ttatcgcagg taggttctgc ggcgtaacgc accaggcgaa aacgctgacc gtcaaagcgc
    90661 cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca
    90721 cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctggccgttg
    90781 ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg gctttttacg cgacacaatc
    90841 catgccaaat caatggtgtt ataggcccct gcctcacagc taatcatcat cagtgcttta
    90901 tcatcggtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga
    90961 ccattcatcc gccagtttcc ataatccagc aaatcgttac gttcttcgag tgagagtggc
    91021 gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgaga
    91081 ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca
    91141 tcaataaaca acaacgccgc ttttaagccg ctcagagaaa tggtctgatt gccgtcgcgt
    91201 aaggtgatcg ccttcccttc ctgaattatc tgcaaaaacg cggtgatggt tgccgtatca
    91261 tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaatgc caacggctca
    91321 ccatctaata gcagccgtgg cgctatctcc ccttcagacg catccggcga cttcaaccca
    91381 ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccag cgctgcggct cagggtcatc
    91441 accagtccat tatgatcgcc cgtattgcgc gccacacaaa aattttgatt attacaggtg
    91501 acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac
    91561 aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt
    91621 caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt
    91681 cggattgagc caataataaa caacgaaata ttattgctat ttttcagtcc attaaatgtg
    91741 acatttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga
    91801 tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac
    91861 cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct
    91921 cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat
    91981 agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca
    92041 gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac
    92101 ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac
    92161 cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct ttgcgcgtga
    92221 gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga
    92281 gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat attacgctgt
    92341 gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag
    92401 tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca
    92461 ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg
    92521 ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc
    92581 cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca
    92641 aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga
    92701 cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc aagcgccacc
    92761 ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac ctctttcttc
    92821 gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg
    92881 cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta
    92941 agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta
    93001 gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa
    93061 cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa
    93121 agcaaatcca aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc
    93181 gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg
    93241 ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac cgccacgcca
    93301 ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat aatcgccacc
    93361 cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc caacgcctga
    93421 tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta
    93481 caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg
    93541 ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc
    93601 cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgaac
    93661 ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca
    93721 tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt
    93781 gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt actggcatgc
    93841 cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat gccggagagt
    93901 gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg
    93961 ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg
    94021 acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca ggatttacag
    94081 ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta
    94141 gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc
    94201 tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc
    94261 tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga
    94321 ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt
    94381 ctttatatga gtgttgttat tacccgactt acaggggaac cggcaatgtt catgtgccca
    94441 gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa
    94501 ttgcgtttca gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt
    94561 aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt
    94621 ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc
    94681 aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt gcaaagtggc
    94741 agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta
    94801 accaacctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat
    94861 gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc
    94921 aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct
    94981 gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg
    95041 ctggtctttt ttactttgcg ccgcatacgc catcaggtgg ttgccccgct gaatcagctg
    95101 gttaccgcca gtcagcgtat tgaacacggg cagttcgact cgccgccgct ggataccagt
    95161 ctgccgaatg agcttggtct gcttgcaaaa acctttaacc aaatgtcgag cgagctgcat
    95221 aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgagacc
    95281 aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac accagccaga
    95341 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcagctgaat
    95401 atctggagct gaacgttggg gataactggc ggattagcga agggaagcca aaccctgagc
    95461 tgccaatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc
    95521 aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg atgctgggac
    95581 gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac
    95641 gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtgctttct tacttacgta
    95701 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    95761 tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta
    95821 ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata
    95881 cgttacaaaa tcaaaccagc gctaaactga ccctcgactg ccgtctgcca accctggcac
    95941 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    96001 tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc
    96061 acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc
    96121 attatggact gaatatcatg cgcgaacgcg cggaacggct gggcgggacg ctgacttttt
    96181 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgca gagggtgagg
    96241 ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc ctctcccttt
    96301 aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc agcaatctca
    96361 tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg ccgctacagt
    96421 gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta atggcgaatt
    96481 tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt ctgacaggta
    96541 ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca ccgaatgtgc
    96601 gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg
    96661 ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct cagagcagtg
    96721 gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg gacgaagccg
    96781 tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa
    96841 atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt gccttcgtct
    96901 ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt aatattcagg
    96961 acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg caatattcca
    97021 tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct aaagatgtca
    97081 ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt
    97141 ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg caaacaccag
    97201 aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta aggctgggcg
    97261 atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc ttcaatggta
    97321 agccagcctc cgggttgggg gtaaaactgg cctccagcgc aaacgaaatg gcgacagcgg
    97381 agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga ctggaataca
    97441 aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc
    97501 tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa aacttccgcg
    97561 ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc tccgtacttt
    97621 acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc
    97681 tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg agtgaggaag
    97741 gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg gcactggtcg
    97801 ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc ggcaccaccg
    97861 gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg tcagtactgg
    97921 tagcgatgct cctcactccg gctctgtgtg ccacgctact taagccactg aaaaaaggtg
    97981 agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac cgcaacgccg
    98041 aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg attgtgattt
    98101 atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg tcgttcttac
    98161 cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt tcaacccaac
    98221 aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat gaaaaagaca
    98281 acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg caaaacgtgg
    98341 cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc ggcacctcgt
    98401 ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct cgcgttatcg
    98461 ccagcagccc accagcaatt agcggtcttg gtagttctgc aggttttgat atggagttgc
    98521 aggaccacgc tggagcgggt cacgatgcac tgatggcagc acgtaatcag ttgctggcgc
    98581 tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat gacagtccgc
    98641 agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat atcgacgata
    98701 ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt atggatcgcg
    98761 gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca
    98821 tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct gctttcgcga
    98881 cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat tctgcggttg
    98941 agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat attatggaat
    99001 cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg tcgtatcagg
    99061 agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg gtggtattcc
    99121 tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg ctggtcgtgc
    99181 cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa aacgacgttt
    99241 acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc atcctgatcg
    99301 tcgagtttgc taacgagatg aaccaaaaag gccatgacct gtttgaagcg acgctccacg
    99361 cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc ttcggcgtat
    99421 tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg
    99481 taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg ctgttctttg
    99541 tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga
    99601 catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc gggttcttgc
    99661 tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag tctttccagt
    99721 tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt ctacggaaac
    99781 aatataccgt ggtgaagcta atttactcga ttgctgcgat gact
//
LOCUS       DAECOD010000002        77385 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.002, whole genome shotgun sequence.
ACCESSION   DAECOD010000002 DAECOD010000000
VERSION     DAECOD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.002"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..301)
                     /locus_tag="KKO15_000098"
     CDS             complement(<1..301)
                     /locus_tag="KKO15_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD6359040.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
     gene            complement(566..826)
                     /locus_tag="KKO15_000099"
     CDS             complement(566..826)
                     /locus_tag="KKO15_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6359041.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            1102..2028
                     /gene="rlmF"
                     /locus_tag="KKO15_000100"
     CDS             1102..2028
                     /gene="rlmF"
                     /locus_tag="KKO15_000100"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBD6359042.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(2025..4249)
                     /gene="ybiO"
                     /locus_tag="KKO15_000101"
                     /pseudo
     CDS             complement(2025..4249)
                     /gene="ybiO"
                     /locus_tag="KKO15_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(4510..5232)
                     /gene="glnQ"
                     /locus_tag="KKO15_000102"
     CDS             complement(4510..5232)
                     /gene="glnQ"
                     /locus_tag="KKO15_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBD6359043.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(5229..5888)
                     /gene="glnP"
                     /locus_tag="KKO15_000103"
     CDS             complement(5229..5888)
                     /gene="glnP"
                     /locus_tag="KKO15_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBD6359044.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(6027..6773)
                     /gene="glnH"
                     /locus_tag="KKO15_000104"
     CDS             complement(6027..6773)
                     /gene="glnH"
                     /locus_tag="KKO15_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBD6359045.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            complement(7177..7680)
                     /gene="dps"
                     /locus_tag="KKO15_000105"
                     /gene_synonym="pexB"
     CDS             complement(7177..7680)
                     /gene="dps"
                     /locus_tag="KKO15_000105"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBD6359046.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(7979..8866)
                     /gene="rhtA"
                     /locus_tag="KKO15_000106"
     CDS             complement(7979..8866)
                     /gene="rhtA"
                     /locus_tag="KKO15_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBD6359047.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            9101..9166
                     /locus_tag="KKO15_000107"
     CDS             9101..9166
                     /locus_tag="KKO15_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359048.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            9219..9734
                     /gene="ompX"
                     /locus_tag="KKO15_000108"
     CDS             9219..9734
                     /gene="ompX"
                     /locus_tag="KKO15_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBD6359049.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(9782..11365)
                     /gene="opgE"
                     /locus_tag="KKO15_000109"
     CDS             complement(9782..11365)
                     /gene="opgE"
                     /locus_tag="KKO15_000109"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD6359050.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(11637..11765)
                     /gene="mntS"
                     /locus_tag="KKO15_000110"
     CDS             complement(11637..11765)
                     /gene="mntS"
                     /locus_tag="KKO15_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBD6359051.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            11951..12418
                     /gene="mntR"
                     /locus_tag="KKO15_000111"
     CDS             11951..12418
                     /gene="mntR"
                     /locus_tag="KKO15_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBD6359052.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            12415..13533
                     /locus_tag="KKO15_000112"
     CDS             12415..13533
                     /locus_tag="KKO15_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBD6359053.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(13591..14511)
                     /gene="ldtB"
                     /locus_tag="KKO15_000113"
     CDS             complement(13591..14511)
                     /gene="ldtB"
                     /locus_tag="KKO15_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6359054.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            14730..16322
                     /locus_tag="KKO15_000114"
     CDS             14730..16322
                     /locus_tag="KKO15_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBD6359055.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(16563..17828)
                     /locus_tag="KKO15_000115"
     CDS             complement(16563..17828)
                     /locus_tag="KKO15_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBD6359056.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSRALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(17980..18795)
                     /gene="ybiV"
                     /locus_tag="KKO15_000116"
     CDS             complement(17980..18795)
                     /gene="ybiV"
                     /locus_tag="KKO15_000116"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBD6359057.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(18941..21373)
                     /locus_tag="KKO15_000117"
     CDS             complement(18941..21373)
                     /locus_tag="KKO15_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBD6359058.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(21379..22278)
                     /locus_tag="KKO15_000118"
     CDS             complement(21379..22278)
                     /locus_tag="KKO15_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBD6359059.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            22409..23071
                     /gene="fsa"
                     /locus_tag="KKO15_000119"
     CDS             22409..23071
                     /gene="fsa"
                     /locus_tag="KKO15_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD6359060.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            complement(23250..23999)
                     /gene="moeB"
                     /locus_tag="KKO15_000120"
     CDS             complement(23250..23999)
                     /gene="moeB"
                     /locus_tag="KKO15_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBD6359061.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(23999..25234)
                     /gene="moeA"
                     /locus_tag="KKO15_000121"
     CDS             complement(23999..25234)
                     /gene="moeA"
                     /locus_tag="KKO15_000121"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBD6359062.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            25438..26403
                     /gene="iaaA"
                     /locus_tag="KKO15_000122"
     CDS             25438..26403
                     /gene="iaaA"
                     /locus_tag="KKO15_000122"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD6359063.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            26390..28261
                     /gene="gsiA"
                     /locus_tag="KKO15_000123"
     CDS             26390..28261
                     /gene="gsiA"
                     /locus_tag="KKO15_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBD6359064.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            28281..29819
                     /gene="gsiB"
                     /locus_tag="KKO15_000124"
     CDS             28281..29819
                     /gene="gsiB"
                     /locus_tag="KKO15_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBD6359065.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            29837..30757
                     /gene="gsiC"
                     /locus_tag="KKO15_000125"
     CDS             29837..30757
                     /gene="gsiC"
                     /locus_tag="KKO15_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBD6359066.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            30760..31671
                     /gene="gsiD"
                     /locus_tag="KKO15_000126"
     CDS             30760..31671
                     /gene="gsiD"
                     /locus_tag="KKO15_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBD6359067.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            31849..34197
                     /locus_tag="KKO15_000127"
     CDS             31849..34197
                     /locus_tag="KKO15_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6359068.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            34205..35533
                     /locus_tag="KKO15_000128"
     CDS             34205..35533
                     /locus_tag="KKO15_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD6359069.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(35580..36905)
                     /gene="rimO"
                     /locus_tag="KKO15_000129"
     CDS             complement(35580..36905)
                     /gene="rimO"
                     /locus_tag="KKO15_000129"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBD6359070.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            37118..37501
                     /gene="bssR"
                     /locus_tag="KKO15_000130"
     CDS             37118..37501
                     /gene="bssR"
                     /locus_tag="KKO15_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBD6359071.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            37612..38726
                     /gene="yliI"
                     /locus_tag="KKO15_000131"
                     /pseudo
     CDS             37612..38726
                     /gene="yliI"
                     /locus_tag="KKO15_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(38723..39349)
                     /gene="gstB"
                     /locus_tag="KKO15_000132"
     CDS             complement(38723..39349)
                     /gene="gstB"
                     /locus_tag="KKO15_000132"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBD6359072.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            39595..40797
                     /gene="dacC"
                     /locus_tag="KKO15_000133"
     CDS             39595..40797
                     /gene="dacC"
                     /locus_tag="KKO15_000133"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD6359073.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(40844..41602)
                     /gene="deoR"
                     /locus_tag="KKO15_000134"
     CDS             complement(40844..41602)
                     /gene="deoR"
                     /locus_tag="KKO15_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBD6359074.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(41660..42256)
                     /gene="ybjG"
                     /locus_tag="KKO15_000135"
     CDS             complement(41660..42256)
                     /gene="ybjG"
                     /locus_tag="KKO15_000135"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD6359075.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            42541..43767
                     /gene="mdfA"
                     /locus_tag="KKO15_000136"
     CDS             42541..43767
                     /gene="mdfA"
                     /locus_tag="KKO15_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBD6359076.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(43808..44092)
                     /locus_tag="KKO15_000137"
     CDS             complement(43808..44092)
                     /locus_tag="KKO15_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359077.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(44178..44993)
                     /gene="ybjI"
                     /locus_tag="KKO15_000138"
     CDS             complement(44178..44993)
                     /gene="ybjI"
                     /locus_tag="KKO15_000138"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBD6359078.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(44993..46201)
                     /locus_tag="KKO15_000139"
     CDS             complement(44993..46201)
                     /locus_tag="KKO15_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359079.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            46285..46821
                     /gene="rcdA"
                     /locus_tag="KKO15_000140"
     CDS             46285..46821
                     /gene="rcdA"
                     /locus_tag="KKO15_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBD6359080.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(46996..48681)
                     /locus_tag="KKO15_000141"
     CDS             complement(46996..48681)
                     /locus_tag="KKO15_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBD6359081.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            48951..49328
                     /locus_tag="KKO15_000142"
     CDS             48951..49328
                     /locus_tag="KKO15_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBD6359082.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(49358..49615)
                     /gene="grxA"
                     /locus_tag="KKO15_000143"
     CDS             complement(49358..49615)
                     /gene="grxA"
                     /locus_tag="KKO15_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBD6359083.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            49775..50062
                     /locus_tag="KKO15_000144"
     CDS             49775..50062
                     /locus_tag="KKO15_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBD6359084.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            50046..50768
                     /gene="nfsA"
                     /locus_tag="KKO15_000145"
     CDS             50046..50768
                     /gene="nfsA"
                     /locus_tag="KKO15_000145"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBD6359085.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDFLHKQGWATR"
     gene            50829..51731
                     /gene="rimK"
                     /locus_tag="KKO15_000146"
     CDS             50829..51731
                     /gene="rimK"
                     /locus_tag="KKO15_000146"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBD6359086.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            51819..52295
                     /locus_tag="KKO15_000147"
     CDS             51819..52295
                     /locus_tag="KKO15_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBD6359087.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            52647..53759
                     /gene="potF"
                     /locus_tag="KKO15_000148"
     CDS             52647..53759
                     /gene="potF"
                     /locus_tag="KKO15_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBD6359088.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            53854..54987
                     /gene="potG"
                     /locus_tag="KKO15_000149"
     CDS             53854..54987
                     /gene="potG"
                     /locus_tag="KKO15_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBD6359089.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            54997..55950
                     /gene="potH"
                     /locus_tag="KKO15_000150"
     CDS             54997..55950
                     /gene="potH"
                     /locus_tag="KKO15_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBD6359090.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            55947..56792
                     /gene="potI"
                     /locus_tag="KKO15_000151"
     CDS             55947..56792
                     /gene="potI"
                     /locus_tag="KKO15_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBD6359091.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            56852..57340
                     /locus_tag="KKO15_000152"
     CDS             56852..57340
                     /locus_tag="KKO15_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBD6359092.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            57381..58508
                     /gene="rlmC"
                     /locus_tag="KKO15_000153"
     CDS             57381..58508
                     /gene="rlmC"
                     /locus_tag="KKO15_000153"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBD6359093.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(58683..59414)
                     /gene="artJ"
                     /locus_tag="KKO15_000154"
     CDS             complement(58683..59414)
                     /gene="artJ"
                     /locus_tag="KKO15_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBD6359094.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(59706..60374)
                     /gene="artM"
                     /locus_tag="KKO15_000155"
     CDS             complement(59706..60374)
                     /gene="artM"
                     /locus_tag="KKO15_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBD6359095.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(60374..61090)
                     /gene="artQ"
                     /locus_tag="KKO15_000156"
     CDS             complement(60374..61090)
                     /gene="artQ"
                     /locus_tag="KKO15_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBD6359096.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(61097..61828)
                     /gene="artJ"
                     /locus_tag="KKO15_000157"
     CDS             complement(61097..61828)
                     /gene="artJ"
                     /locus_tag="KKO15_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6359097.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(61846..62574)
                     /gene="artP"
                     /locus_tag="KKO15_000158"
     CDS             complement(61846..62574)
                     /gene="artP"
                     /locus_tag="KKO15_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBD6359098.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(62792..63307)
                     /locus_tag="KKO15_000159"
     CDS             complement(62792..63307)
                     /locus_tag="KKO15_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6359099.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            63956..65725
                     /locus_tag="KKO15_000160"
     CDS             63956..65725
                     /locus_tag="KKO15_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD6359100.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            65936..66259
                     /locus_tag="KKO15_000161"
     CDS             65936..66259
                     /locus_tag="KKO15_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBD6359101.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            66256..67086
                     /gene="amiD"
                     /locus_tag="KKO15_000162"
     CDS             66256..67086
                     /gene="amiD"
                     /locus_tag="KKO15_000162"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBD6359102.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(67083..68096)
                     /locus_tag="KKO15_000163"
     CDS             complement(67083..68096)
                     /locus_tag="KKO15_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD6359103.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(68195..69625)
                     /locus_tag="KKO15_000164"
     CDS             complement(68195..69625)
                     /locus_tag="KKO15_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBD6359104.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(69636..70637)
                     /gene="ltaE"
                     /locus_tag="KKO15_000165"
     CDS             complement(69636..70637)
                     /gene="ltaE"
                     /locus_tag="KKO15_000165"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBD6359105.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(70674..72392)
                     /gene="poxB"
                     /locus_tag="KKO15_000166"
     CDS             complement(70674..72392)
                     /gene="poxB"
                     /locus_tag="KKO15_000166"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBD6359106.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(72525..73493)
                     /gene="hcr"
                     /locus_tag="KKO15_000167"
     CDS             complement(72525..73493)
                     /gene="hcr"
                     /locus_tag="KKO15_000167"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBD6359107.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(73505..75157)
                     /gene="hcp"
                     /locus_tag="KKO15_000168"
                     /gene_synonym="priS"
     CDS             complement(73505..75157)
                     /gene="hcp"
                     /locus_tag="KKO15_000168"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBD6359108.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(75301..76200)
                     /gene="lysO"
                     /locus_tag="KKO15_000169"
     CDS             complement(75301..76200)
                     /gene="lysO"
                     /locus_tag="KKO15_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBD6359109.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(76657..77352)
                     /gene="aqpZ"
                     /locus_tag="KKO15_000170"
     CDS             complement(76657..77352)
                     /gene="aqpZ"
                     /locus_tag="KKO15_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBD6359110.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT        19079 a        19017 c        20165 g        19124 t
ORIGIN      
        1 cgccggggac gtttttgagc gcgtcggtaa ggttggttgc gccctgatct ttaatcactt
       61 gttcagaaat caccgtcatc gtgcgggtag tatccgctac cggacgcgag aatttcggat
      121 cggcagattg ttgtggcgca taaagcgaag gcgttgatgc ttcgacaacc agcgtgtcat
      181 ccgcgttagc ttgcccttcg gcggcgagtg cctgtgccac aggcgtaata ccaatacaaa
      241 gaccggcaaa gaacgtgagc gaatgaaatt gtctggcagg gaaattgcga ttgttttcca
      301 ttttgcaggt gactttttct tatatgttga aattttcact gccacgagaa agcatctctg
      361 cggatgcgat gccactgtta atcccgtgat agtgttcgtg caagtgataa taattttgat
      421 aagcattatc acttgatgcg attttctatc acccattgcg cgaaaaagaa atacatttat
      481 ttacattgcg ttcatatttg atgttttttg attacaaaac aggcgaaaac tgcatccaga
      541 ggcgaatgca gttacgttgg tggggttatt tgtagatggt tgcagtacct aacatctggt
      601 catttccgcc tgcggaatta atgacataac ctttcgcgcc ttgttcgcgc gctttttctg
      661 ccagtttatc ctccagatcg cttaggtttg atgcgccagt tgccgaaacg gtaccggcgg
      721 gccgtagctg accgctgtcc aggttactca tcggttgcgc ggcataagcc gtgagagaga
      781 ttccgctcag gacggtggca atcagtagtg tgaggcactt tttcataatg acatcctcat
      841 aaatacaacg ggtagtgcct gataagtgta ggtcgcctgc gcgatgcaaa tgacgcaaaa
      901 aattgacaga gatatgctta ttttttgaac gatatatttt tacaaaataa gacaaatcaa
      961 tgacatgcat aatgctgatt tttatgatga attgccataa accatagcca tgttaaggtt
     1021 ttggggaatg ataaacgcag ccagatattt accctgtcgc gttatgcggg tatcttacgc
     1081 cgctgcttaa aggagaatgc tatgtccgcc cagaaaccgg ggttgcatcc gcgcaaccgt
     1141 catcacagcc gctacgatct cgccacgctt tgtcaggtca atcctgaact caggcaattc
     1201 ctcacgctta cacccgcagg ggagcaaagc gtagactttg ccaatccgct ggcggtgaag
     1261 gcgctcaata aggcgttgct ggcccatttt tacgccgtag cgaactggga tatccccgac
     1321 ggttttctct gcccaccagt accgggccgg gcggattata ttcatcacct tgccgattta
     1381 ctggctgaag caagcggaac aattccagcg aatgccagca ttctggatat cggcgttggt
     1441 gcgaactgta tttatccgct gattggcgta catgaatatg gctggcgttt taccggtagt
     1501 gaaaccagca gccaggcgtt aagcagtgcg caggcgatta tcagtgctaa tccggggctt
     1561 aaccgcgcca ttcgtctgcg tcggcaaaaa gagagtgggg cgatttttaa cggcatcatc
     1621 cacaaaaacg agcaatacga cgcgaccttg tgtaacccgc cattccacga ttccgccgct
     1681 gcggcgcggg caggtagtga gcgtaaacgc cgtaacctgg ggctgaacaa agacgatgca
     1741 ctgaactttg gtggtcagca acaggagttg tggtgtgaag gcggtgaagt caactttatc
     1801 aaaaagatga ttgaagagag caaaggcttc gcgaagcagg tgatgtggtt tacctcgctg
     1861 gtatctcgtg gtgaaaactt accgccgttg tatcgtgctc tgaccgacgt gggcgcggtg
     1921 aaggtggtaa aaaaagagat ggcccagggg caaaagcaga gtcgctttat tgcctggacc
     1981 tttatgaacg acgagcagcg ccgccgtttt gttaatcgcc aacgttaaag cgttggctcc
     2041 cccggcggca gcggttcagc cggggtcgcg cccggagcag gcagcacctg ataagtctgc
     2101 actggcgcgc gaacgcccgc caggtcgaaa tgttttttca cctggctgtc gagggcaaag
     2161 cgtaccgtcc actgtttgag tggcagcgtg gtgaacgaaa catgcagtgt aaacgcggta
     2221 ttgcttaagc cgacaatccc ggcaaagttc ggttcaccaa taatcagccc gcgaatttct
     2281 tcgttttcca tcaattccgt taccgcatct ttcagtgcct gattggcttt atcagcatct
     2341 tcatggcgat caacatcgta gtttgccact accgaaccaa taccgcggac gaagttagca
     2401 aaggtggtta tcgaagacca cggaatgatg tgatacgccc cggtatcctg gcgcacgccc
     2461 acggagcgaa tcgacatccg ttccactgtg ccggtcaacg gcccgatagt caccaaatct
     2521 ccagtgttca tgccgttttc aaactgaata aataccccgg tgataatatc ttttaccagc
     2581 gtttgcgaac caaacgagat agccagacct aatgccccgg cacctgccag caatggcgcg
     2641 atattgacgc cgatttccga caacacaatc atgatggtga tggtactgat aatcaccgcc
     2701 agcgcgttac ggaacagcgt cagcagggta cgcgtacggg cgctgggtag cgggcggcca
     2761 tgaatatccg aagccagccg gttttcgatc aaactggcga gcaccgtcca gccaaccgcc
     2821 gagaagaaaa gaatgagtgc gatgcggatc aggatatcta cggttttctg ccccgcgccg
     2881 ttttgcagcc agttccagaa atcgaacaat ccccatgcgc tcaacagcaa cattactgcc
     2941 acgcagacgg tcagaatacg cgccgttttc agcgccgccg aaagccagcc attcagccgt
     3001 ttttgtagct ccggatagtt acgctgagta tgtggcgaga gggtgatggt tttcgccagc
     3061 cagcgggaaa acataccgga aacaaacgct gcgataccaa taatcgccag gctgcgcacc
     3121 gttgcaccca tcataaattt cagactgttg cccggatcga acaacgaaaa gaaaaacagc
     3181 atgataaaat aggcgcttgc cagccagtgc cacaccagcg caaaggcgcg gataaacagg
     3241 ctgaaaaaag ccagcgaatg ctccgcaaag ttgagcatat gctgggtaat ctctttttta
     3301 tttcggaaga tcaggtacaa cgcccagacg gtcatgcaca gcataatgat gacgttcgcc
     3361 agcgcaccta tctgtacatt cacctgatta gagataatcg gcacggccac aatcaggcca
     3421 tagccaatca ggctgcttaa ccagctcagg cgacgacccc agtaacgggc gctctcgtcc
     3481 tgaatcgtga acgggcgcag ctccgccacg tttgggcaaa aaatcaggcg tagtacggct
     3541 ttgaaaaatt caatcagggc aaaggcgttg agaaacaaac tttgttggaa agcgatggtg
     3601 cgactgcctg cattcaggtt gtcgcttaat acctggccga caaataatgt cagggccagt
     3661 aacagcaggt cgataataaa cgccccaata atcatcgccg gaagctgcaa ccagttgctg
     3721 cgctcacgat ttttttgcct cgcccactga cccattttgc gatatagcgg cagtgcgcac
     3781 aggcgaatca gccagtaaaa accaaacact aataccgcta acattgaaaa atggctcagc
     3841 gcattgctga aggtttgtgg attaaatggc ttatgcgggg agccggtgat attgcgataa
     3901 agttgcccga agcgggcgga aagggcttca ccataatggc ggctgacttc ggtgaccttt
     3961 tgcagcacgg tttgctcttc aaccagtgtc ggcggcacga tcttcgggac cggttccgca
     4021 gggggcgtcg cggcgacagt acgcaactgg tcgatcaact ctttacgcga ggtgtcgtta
     4081 tccagcacat ccgccagtgc gccataggcc gctttttttg ttcgatatcc ggttccgggg
     4141 ccggttcagt cgttgagtcc gttgtcgttg tggttgtaac gccgggtatg gataccgcgt
     4201 gggcaggtgc gcccagcagg cagaagagga tgaacaggat ccaccgcatg actcctccag
     4261 tgagaaaaca gagcaaaaag ataagtatag atgctggggg aggggcgatt ttaaatgaga
     4321 ggaatctggt gtgcctccct ttcgggtgaa agggaggaag ggatattaag caggttgctg
     4381 acaacgtgcg ggcttttttg ccggatgcgg cgtgaacgcc ttatccgtcc tacaagttca
     4441 tgcaatttta ataagttgcc agatatcctg taggcctgat aagcgtagcg catcaggcaa
     4501 tgtgtcttat taagagacgt gctgcaaaaa ttcctgcaag cgctggctcg gcgggttctt
     4561 gatcaacacc tgcggattgc catcttccgc aatccggcct ttgtcgataa agatcagccg
     4621 cgaagctact ttctcggcaa aaccgatttc gtgggtcacg atcaccatcg tcatcccttc
     4681 ttcagccaga tcctgcataa ccttcagcac ttcatggcgc agttccgggt caagagcgga
     4741 agtcggttca tcaaacagca tcattttcgg cttcaccgcc agcgcgcggg caatcgccac
     4801 acgctgctgt tgaccaccag aaagttcgga agggtagtga tgtgcacgtt ctgccagacc
     4861 gactttcgcc agcagctcac gcgccagttt ttccgcctct tctttgttcg cgccacgcac
     4921 gcgtagcgga ccaaacatga cgtttcccag cgccgtcaga tgcgggaaga ggtaaaactg
     4981 ctggaacacc atacctgctt cctggcgaat caggcgctcg tcaactttcg gatcgttgac
     5041 cttcaggcca tcgacaatca gatcgccgga ggtgatttct tccagtttgt tgatgcagcg
     5101 cagcagggtc gatttaccgg aaccggacgg cccgataatc accacgactt cgccctgggc
     5161 aatgttcaaa tcgatattgt gcagcacctg ggttgggcca aagtgcttgg agacgttttt
     5221 aaattcaatc acaggatttt catccttctt tccagacgac gcagaataaa gctcagcacc
     5281 agggtaataa tcagatagaa caccgccacg gcgctccaga tctcaagggc gcggaagtta
     5341 ccggcaataa tttcttgccc ctgacgggtc agttccgcca cgccgataac aataaacagc
     5401 gaggtgtctt taatgctgat gatccactgg ttacccagcg gcggcagcat acgacgcagt
     5461 gccagcggta aaatgacgta gcgaatggtt tcccaacgtg aaagaccgag cgccagtcct
     5521 gcttcacgaa aacctttgtg gatagacagc accgcaccac gcgtgatttc cgcaatgtac
     5581 gcgccggagt tgatcatgat ggtgaccacc gccgcagtaa atgggtcgat gcgtaagtcg
     5641 ttaaacgcca tcggcagggc gaaatagata aacatcacct ggacgacgat aggtgtgccg
     5701 cggatcactt caataaagac cagcgcgacg tggttggcta tccaaccacc gaaggtgcgt
     5761 gcaaaacctg ccagcaatcc gattaccaga ccgccagcca gaccgaggac cgaaatccac
     5821 agggtcattt tggcaccttc aatcagaagc ggaatggcag gccagatggc actccagtca
     5881 aactgcatat gttgttcctg ttaccgtggt gtaaataatc aaattcaggg taattaatgg
     5941 ccccggagcg ggtttgcgcc cctcacccta atcctctccc caggggggag agggaactgc
     6001 cagtgcgctt tacaggtgta gcgttattat ttcggttcag tactgaacca ttttttgtag
     6061 atttcgttgt aagttccgtt ctcgcgcagg gttttcaacg cgccgttgac tttgtcacgc
     6121 agctcgtcgc tacctttcgg gaacgcaata ccgtattgct gcgcttccag agagtcacct
     6181 accgctttga actgaccgtt accggcggtt ttgatgaagt acagaatgtt tggcgtatcg
     6241 tgcagaacgg cgtctgcgcg gttggtgccc agttccatat aggcgttatc gatgttcggg
     6301 aactgacgca gatctttagt tttgatgttt gctttcgcgt aatcaacgga gccagtaccg
     6361 ctcttcacag caaccacttt cccgtcgaga tctttcacgc ttttcacatc gttattgtta
     6421 gctttcacca tcactaacag gccgcttttg tagtagccgt cagagaaatc gatcgctttt
     6481 ttacgctcgt cggtgatggt aatgcccgcc agcgccagat cgacgttttt ggtttgcagt
     6541 gccggaatga tcccactgaa atccatcggc ttcagttcgt aatccagctt cagctcttta
     6601 gcgatggcag cccacagatc aacgtcaaag cccacatatt tatcgccctg tttaaattca
     6661 aacggaacga aggcggtatc cgtcgcgaca actaattttt tatccgcggc atgagaagaa
     6721 accgcaaaag ccagggtcag tgcagccagt gaaactttta atacagactt catagcattt
     6781 ccttttttct atccacggga cgatcccctg cgtgacattc acatatatga aaaaatcgtg
     6841 ccaattttgc aactcattga tttttcgaga tgcggcaaaa acgtgatgca cgatttatag
     6901 ggcaataccc tgaagatgca ccattctggg gcaccaatct ggtgcgctaa aattgtgcac
     6961 tcaacacagt gattatttag cgtaaaacct gatgaaaaaa caatctttat gtaacgattg
     7021 tgtgatgatg tggatacaaa aaatttactt aatcagctgg ggatagcaga tggatgcact
     7081 aaataagtgc gttgaggtgg gctgcaaaac aaaacggcct cctgtcagga agccgctttt
     7141 atcgggtact aaagttctgc accatcagcg atggatttat tcgatgttag actcgataaa
     7201 ccacaggaat ttatccaggt cgcgagacgc ggcggtcagg atatctgcgg tgtcgtcatc
     7261 tttcgcttcg ccaatcgctt tgcgtacgtc attagcgacg attgcgtaac ggtcagccag
     7321 ttctttcagg tgatcctgaa cgttgtggat gtccagcggg taacttttca gcggggtttt
     7381 gctgttgata acttgagtgg tccccagagc tacaccgccc agctgcacgg cacgttctgc
     7441 catggtatcc agatgatcga tcagtgcggt gcggaagcca tccagcattt catgtacggc
     7501 aatgaagtta gcgccgcgca tgttccagtg cgcttgtttg gtaatcaaag aaagatcaat
     7561 aaactggata acctggcgat tcagcaactc tactgttgct tttttctcgc tgtcggagac
     7621 atcgttgcgg gtataaagca gattggtcgc ttttgattta actaatttag cggtactcat
     7681 aatttcatat cctcttgatg ttatgtccca gtaattaacg agactaagta tagcaccggc
     7741 tatgtgttcc gctattctgg ctgttcctat cacactaata gtggtaacaa gcgtgaaaaa
     7801 caaaactaat aaagattcaa tgagttagat atattgataa gaacaattct tatttatctg
     7861 ctcgccagga attttcggaa taaatatgct tttgcataat tcgatatata aatacggtgt
     7921 agaggaaaag tagcgagaaa ttctgcatgg ttatgcataa ccatacagaa atgtaaattt
     7981 aattaatgtc taattctttt attttgctct ctttgcgtac tgtcagcgta gaccccattg
     8041 aagcggcgat gatagcgccg agcgccagca gctgtatggg tgtcagtgtt tctccgagga
     8101 aaatcatccc ggaaacggca gccagcgccg gttccatgct catcagcgta ccaaatgtcc
     8161 gtgttggcaa acgggtgagg gcaatcattt ccagcgaata aggcagagcg gtcgagagaa
     8221 tagcgacagc cagacccaat ggaataaccg accagtgcca gagtgcttca ccagcctgaa
     8281 gcgctccaat tggcacgaaa attaacgctg caatcaacga accaattgct accgtcgcag
     8341 ggccatgttc cgctcctgcg cgttgcccac ttaaaatgta aatagcccaa caagccccgg
     8401 cccccagtgc cagcgcacag ccggttaaat cgacatggga aacgtcttgc cccagcggta
     8461 gcaggaacca cagaccaaga accgccagca caacccagac gaaatctacc gggcgacgag
     8521 aagagaacag tgccaccgcc agtggtccgg tgaactccag cgccaccgca atacccagcg
     8581 gtactgtctg aatagaaaga taaaaaagat aattcatccc acccagcgaa acgccgtaaa
     8641 acaacagcgg taaccgttgc tctttggcaa agcgcagtcg ccatggctta aagaacgcga
     8701 tgaggatcag cgttcctaat gccagacgca gcgcagtgac acccggtgcg ctcaccagag
     8761 gaaaaagtga cttagctaac gaggctccac cctgaataga cgccatggca acgagcaata
     8821 tgactattgg taaccagacc ggcattttac gtaatgaacc aggcatcctt tctcccacaa
     8881 atatctagac ttaagtaaag cgtggagtgt actggatata cccaatgctg gttgagcatt
     8941 tgttgaaaaa attttccccc gttttgacta aaatgcgcca ggattgatgg aatcattagt
     9001 ctggtgatta ggaataatct ggatgaatga cagggaaaac atgcgtaata cttacgcagt
     9061 tctctgaaaa agtgatttaa atttagatgg atagcggtgt atggaaacgt tctgttacat
     9121 gaaatggccc gttagacatc acaaatcgcg aagagtttcc cattaatttt tgatatattt
     9181 aaaacttagg acttatttga atcacatttg aggtggttat gaaaaaaatt gcatgtcttt
     9241 cagcactggc cgcagttctg gctttcaccg caggtacttc cgtagctgcg acttctaccg
     9301 taactggcgg ttacgcacag agcgacgctc agggccaaat gaacaaaatg ggcggtttca
     9361 acctgaaata ccgctatgaa gaagacaaca gcccgctggg tgtgatcggt tctttcactt
     9421 acaccgagaa aagccgtact gcaagctctg gtgactacaa caaaaaccag tactacggca
     9481 tcactgctgg tccggcttac cgcattaacg actgggcaag catctacggt gtagtgggtg
     9541 tgggttatgg taaattccag accactgaat acccgaccta caaacacgac accagcgact
     9601 acggtttctc ctacggtgct ggtctgcagt tcaacccgat ggaaaacgtt gctctggact
     9661 tctcttacga gcagagccgt attcgtagcg ttgacgtagg cacctggatt gccggtgttg
     9721 gttaccgctt ctaatcactt tggtgatata aaaatccgcc tctcggggcg gatttttgtt
     9781 tttaaggttt cgggtcgaaa atatcggttc cgagatggtt gtaatccacc ttctgtaact
     9841 ggaagttggt gatgtatatc ggcccggctt tcttctcaga tataaacggg tatttgatat
     9901 ttatttcctt cgctttaatt cccgtccact gcgagaaaaa gcctaagaag tcattggctg
     9961 agcggcgggc tttaatcacc cgatgcgctt tatcgccgct ggaaatgacc ataaaaggca
    10021 cctggaaatt ttgctgatat ttatcatcat gggcaaggta ttgcacgtct ttgccgcgct
    10081 ctttaaaggc cagaccgtgg tcagaaaagt aaaccagaga gaagctgctg ccgctgttgc
    10141 gtaactgatc gtacagcttg cgcagtaaat cgtccgtttg cgtcatggta tagagatagc
    10201 acgacgtttc tttcgattgc acaaaggttt cgtatttgcc ttgtgtcctg tcgcaggcct
    10261 gcggatgcga gcccatcaga tgcagaacaa tcagctgcgg ttgcgagtgc tcttgcgcca
    10321 gcacttgagc ggtcatatcc agtaacgctt cgtctttcgt gtttttatct gcttcaaaat
    10381 taccttcttt caggaagtac acttcatctg cacgtttggc gatgctggcg atagcagtat
    10441 cgtattcgcc gatttggccc tggttggaaa accaccaggt ctggaagccc gcgcgatttg
    10501 ccagggtgac aaagttatcc tgaaactgtg gtttgccatc gactacgcga ttgagcgtta
    10561 agccaagcga tttctgcgtg gagccactgg cagcaatgta gtcagcaaat atcaaaccgt
    10621 taacgctgct ggcaaacggg gtattgtccc agtgaccgcc aaaggcaccg agggcatcgc
    10681 gacgcgcgct ttcaccgatc actaccacat aggtctgata cttcggctta acggcagtga
    10741 ccgtccaggt atctttcatt ccggaaagtt ttgccatccg ttcttgctct tcaatcacct
    10801 cgttattatt gacgatgaca tccttagcaa aacgaaatac cggatagcca gtatctttca
    10861 gtttaaatac gccaccccag gccaggtttt gcacgggagc aacaaaaaat gtcgccacac
    10921 tgaataccag acacaggctg tcgaaggtat tccagcgcgc tttttcattc tctttttttc
    10981 gcctgattgc tattacgccg agcgcaaaaa taaataagcc gaccaggtag ctgtaccacg
    11041 gaaaaatcgt caggatttcg gtcgactctt ccatattggt ggagtgcaat gccagcaatg
    11101 tattgaaatt aggcgcgccg taggcctgag caaaagggaa ataacaagcc gccaccagcg
    11161 aactgacacc gaccagaact ttttgtacgc gaggcaatgt tcgccatagc aaaagcaaaa
    11221 tagccgtaaa cgcagcggtg tagagcaaac tgaaggggta acctaagccg aggttaatta
    11281 atagcgactg taaaaagtag aacgcagtcc acgggctaaa tacccggcta cgggtaacaa
    11341 gcgattcttt gagggttaaa ttcatatgcc actatcgtaa caaacgccat gtgcttaccc
    11401 tggcgtcaag ggtcaatacc tgcaagagtg cgtgaagagg ggaaaggggt ccgcatccgc
    11461 gagccgcaat atacgcaggg ctgcaagaag atagagcgag ccgccgttca ggtcaactac
    11521 tgacgaacaa atcttttgcg aaggggattg caaatccgac aaaaagtggt ttttttcaga
    11581 ccaaaacgcg ttatgcctgc ataaaagcag gagtgaagcg gcgtaccgcg acgccgctat
    11641 ttatctgaag gtttatcttg ctgcggtttg ttgttgacca tatcgcacaa catagagagc
    11701 agcattaacc gtactttaaa gggagaatga ctaaacacgc gcatacacct cttgaactca
    11761 ttcataagac ctcctgactt gctaatcccg tcgatccttg agggatgatt gcattacata
    11821 cagatatagc acaggctata ttatatagct attgctaaaa cgttaatttt ttgtaccttc
    11881 gcaactctgg tttacaatgt gcgcacgaaa tgagaacgct atgtatgcgt caccataatg
    11941 aggaagcaca atgagtcgtc gcgcaggtac gccaatagca aaaaaagtga cgcagttagt
    12001 gaacgtggaa gagcacgttg aagggttccg ccaggtcaga gaggcgcatc ggcgcgagct
    12061 tattgatgat tacgttgagc tgatttctga cttgatcagg gaagtggggg aagctcgtca
    12121 ggtggacatg gctgctcgtc tgggagtttc gcaaccgacg gtggctaaaa tgcttaagcg
    12181 gctggcaacc atggggctga ttgaaatgat cccctggcgt ggcgtgtttt taacggcaga
    12241 aggagagaag ctggcgcagg aaagccgcga gcgacatcag atagtcgaaa atttcttgtt
    12301 ggtgttgggc gtcagtccgg aaatcgcccg tcgcgacgcg gaaggcatgg agcaccatgt
    12361 tagtgaagag acgctcgacg cttttcgttt gtttacccag aaacacggtg ccaaatgagc
    12421 ctgccttttt tacgcacgct gcaaggcgat cgtttttttc agttattaat tcttgttggt
    12481 atcggattaa gctttttcgt gccctttgca ccgaaatcct ggcctgctgc tatcgactgg
    12541 cacaccatca tcaccttaag cggcctgatg ctgctgacca aaggtgtgga gttaagcggt
    12601 tattttgatg tgctggggcg caaaatggtg cgccgctttg ctacggagcg tcggctggcg
    12661 atgtttatgg tgctggcggc ggcgctgctt tctacctttc tgaccaacga tgtcgcgctg
    12721 tttattgttg ttccgctgac tatcacgcta aaaagactgt gtgagatccc ggttaatcgg
    12781 ctgattattt ttgaggcgct ggcagtcaac gctggttcgc tactgacgcc aattggcaac
    12841 ccgcaaaata ttcttatctg gggacgttct ggtctttcgt ttgccggatt tattgcccaa
    12901 atggcaccga tggctggcgc aatgatgctg acactcctgc tgttgtgctg gtgttgtttc
    12961 cctggaaagg cgctgcaata ccatacgggg gtgcaaacac cggagtggaa accgcggctg
    13021 gtgtggaatt gtctggggct gtatatcgtc tttctgacgg cgctggagtt caaacaagag
    13081 ctgtggggac tggtgattgt ggcggcgggc tttgtgctgc tggcgcgtcg cgtggtgttg
    13141 agtgtggact ggacgttgct gctggtgttt atggcgatgt ttatcgacgt ccatttactg
    13201 acccagcttc cagcgttgca aggcgtgttg ggtaacgtga gtcatttatc tgaacccggg
    13261 ttatggttaa cggcagtcgg tttatcgcag gtgatcagta acgtgccgag taccattttg
    13321 ctgctcaatt atgtacctcc gtctttatta ctggcatggg cggtaaacgt ggggggcttt
    13381 ggtttattac caggatcgct ggcaaatttg attgcgctac gtatggcgaa cgatcgccgc
    13441 atctggtggc gtttccatct ctattcaata ccgatgctgt tgtgggcggc gctggtggga
    13501 tacgttttgt tagttatgat cccggcctgg taaggtctgg caaataaaaa aggcggatta
    13561 tgagtccgcc tttgttttac gttttgacga ttaattcagg cgaaccggca tcccggaacg
    13621 atttttgatc gcttcatcaa gaacaacctg gtcaacatct ggctgaccgg tcactgtctg
    13681 cacgctcttc gtcagggtaa ttggcacgat ttcctgacct tcaaactggg cttcggtggt
    13741 agacagcggg ttatggactt caatataacg gctgccgtct ggctcggtgg tcgcttttac
    13801 cggctcatca ataaactgta cgcgggtacc gaccggtact ttctcgaaca ggaatttgat
    13861 gtcttcgtta cgcagacgca cacaaccatg acttacacgc aggccgatac cgaagttggc
    13921 gttggtgcca tggatagcat acaggcgacc gatgtagagt gcatacagac ccatcgggtt
    13981 atccggacct gccggaacga cagccggtag cggttcgccc gcagcgcggt actctgcgtg
    14041 cattttggcg gtcggcgtcc aggtcgggcc tgctttctta tgctcaactt tggtggtcca
    14101 gttgataggc gtatctttgc ctaactgacc aatgccgatc ggcagcacga taacggtgtt
    14161 ggtccctttc ggatagtaat acagacgcat ctctgcactg ttaatgacga tgccttcatg
    14221 aacggtatcc ggcaggatca gctgctgcgg aatgttcagt acagtaccgc ctttcggcag
    14281 gaaggtatcc acacccgggt tcgcttccat catattggaa agccccatct ggtactccgc
    14341 ggcaaaatac tccagcggct gagtgttacc ttcaggaatg gtgatcacct gattctgacc
    14401 aaccaggcga ctcccgtcgg ttggcagagg ataagttacc gcagaggcgg tactgcaaaa
    14461 gccgacaaca gcgaaggccg ctgcgaataa tgttttcaat ttcatattca tgttaagcga
    14521 gattttgtgc ctggcaggcc attgggttga gaatattaga gtattggaag cgcattataa
    14581 gttcattcca gctcacagtg aaatcagatg tgtacgaaat cacatttttt gcctttggct
    14641 tgagtgtaga ccttaagcga ggagcaggat cttctttcag acttatggca taatgcgcgg
    14701 tttgtcatat ctcttttcag gatacgcctg tgttagtttc cagtaacgtc accatgcagt
    14761 tcggcagtaa gccgttgttt gaaaacattt ccgtcaaatt cggcggcggc aaccgttacg
    14821 gcctgattgg cgcgaacggt agtggtaaat ccacctttat gaagattctc ggcggcgacc
    14881 ttgagccgac gctgggtaac gtttccctcg atcccaacga gcgcattggt aagctgcgtc
    14941 aggatcagtt tgcctttgaa gagttcactg tgctggatac ggtgatcatg gggcataaag
    15001 agttgtggga agtgaagcag gagcgcgacc gcatctatgc tttgccggaa atgagtgaag
    15061 aagacggcta taaagtggcc gatctggaag tgaaatacgg cgaaatggac ggttactctg
    15121 cggaagctcg cgccggtgaa ctgttgcttg gcgtgggaat tccagtggaa cagcactacg
    15181 gcccgatgag tgaagttgct cctggctgga agctgcgtgt gcttctggcg caggcgctgt
    15241 ttgctgagcc ggatattctc ctgctcgacg aaccgaccaa caacctcgac atcgacacca
    15301 ttcgctggct ggaacaggtg ctgaacgagc gtgacagcac catgatcatc atctcgcacg
    15361 accgtcactt ccttaacatg gtctgtaccc acatggcgga tctggattac ggcgagctgc
    15421 gcgtttatcc gggtaactac gatgagtaca tgacggcggc gacccaggcg cgtgaacgtc
    15481 tgctggccga taacgccaag aagaaagcgc agattgctga gttgcaatct ttcgttagcc
    15541 gctttagcgc caacgcctcg aaatctcgcc aggcaacttc gcgcgcgcgc cagattgata
    15601 aaatcaaact ggaagaggtg aaagcctcca gccgtcagaa cccgtttatc cgttttgaac
    15661 aggataagaa actgttccgt aacgcgctgg aagtggaagg tctgaccaaa gggtttgata
    15721 acggtccact gtttaaaaat ctcaacctgc tgctggaggt cggtgaaaaa cttgcggtac
    15781 tgggtaccaa cggcgtcggt aaatcaacgt tgctgaaaac gctggtgggc gatctgcaac
    15841 cggacagcgg caccgtaaaa tggtctgaaa acgcgcgcat tggttactac gcccaggacc
    15901 acgaatatga gtttgaaaat gatctgaccg tgttcgaatg gatgagtcag tggaagcagg
    15961 aaggcgatga cgagcaggcg gtacgcagca ttctcggtcg tttgctgttc agccaggacg
    16021 acatcaaaaa gccagctaaa gtgctttccg gtggggaaaa agggcggatg ctgtttggta
    16081 agttaatgat gcagaagccg aacattctga tcatggacga accgaccaac cacctggata
    16141 tggaatccat cgagtcactg aacatggcgc tggaactgta tcagggcacg ctgatctttg
    16201 tttcacacga ccgtgagttc gtaagctccc tggcgacccg cattctggaa atcaccccgg
    16261 aacgcgtgat cgactttagc ggtaattacg aagattacct gcgtagtaaa gggatcgagt
    16321 aagtgattat ccgtcacccc gatactgtcg gggtgacgac ggctgatgac aaatgcaaaa
    16381 tagcctgatg cgctacgttt atcaggccta cgcagtacct gtaatatatt gaatctgcag
    16441 gactttgtag gccggataag gcgttaacgc cgcatcaggc attaagaaag cgcacttgtc
    16501 agcaatctga gtaacgtcga atcgattttc ttaatcccac atcaattcac ttccattaac
    16561 tctcaaacat ccatgcccaa cgctcgctta ccgtgaatgt tgaggtcggc aagcgtaaag
    16621 cgtccttccc agtttgtttc atagtcctcg cgcgggaagt cgcccggcga tgcgcctttt
    16681 tccagtgcgg ccttcacctt gtgcgcgtag gcaagatttt tctcgcacat cggtgccgca
    16741 ggaatgtaca tcacgttgcc ccaaccctgt tgattttcaa cgggggcaac ggaatggatg
    16801 acgtcgcagt gccaccagac ggagtctccg gcctcgagtt ttggaatgct ggttaacgcc
    16861 tcaattaaca gtggatgcca ttgctctgat accggcaaca ctcttccggg cgctacgccg
    16921 cacagttcat cctccggcac atcatcaagc agcggacgta acagtacgta cgccatggct
    16981 tcaggaatgg gcacgacgtg cagcaacccc tgaccaggca gcatatccga gagcgcagtc
    17041 cagccctgga atgtccgaaa cacggaacat ttggtggtgt tgtccaccgt gtactcttca
    17101 acttccgtac gatgtgccgc atgccaggga tcgtatttcg ccagattgcc attaaagacg
    17161 ttggcgaaaa cgtgctgata cgctggaagc agccagcgtt ccagcgccct ggagtcggta
    17221 tgcgctccaa gacctttgga ggtcgttccg ggcggaaggc ggcggatacg gtcagggtag
    17281 atgacgctca catccgggtt aaaccattgc tttccatcac tttcaaatgt ccacagacga
    17341 ttgagaaacg actgcgcatt cgccatttct tcactctggc gggcctgcat ttgcgcctgc
    17401 gaccagtaga tggggtaaat ctcgggacgt gaagcgctga gcgtcccgaa gaaattatcg
    17461 ccggggcctt tgtagacctc gtcaaagcgg ttgcggttca gatagtccag catcgactga
    17521 tcccagccta gcgcttgttc gcgggggaaa tggcctttta tcaccgcaca accgcgacgt
    17581 ttaatctgtt cgcgctgctc tgcagtaaca tgacctgctt tgatatcggc ataagacagc
    17641 accggccaga cggcatcgcc ctgtgctttg agtgcgttga tttcagccac tcgcgtggca
    17701 atgtcatcgc ttagctgatt aaagatctgc tggacatcgc caagctgcgc ccgcagcgcg
    17761 tgcttcatct gacggatagc tgctttgtga tcggcaggca atgtgtcgct ggtaaaagta
    17821 gaagccataa ccacctcgca ttaactttca ttcgaaacta tgctaatttg catgtgacaa
    17881 tacaagttat aaataagtta atgcaagttt aatgatttga tgtgatgtac aggatgtaaa
    17941 aagaagggaa aaccggagcg catggctccg gtagagaggt cagctgttaa aaggggatgt
    18001 gttatccagc acagcctgaa tcacattcag cgcgccttca tgattattat catcggtagc
    18061 gtaacgggcg atttgtttaa tgttttccgc agcattgccc atcgcaaagg aataacgcgc
    18121 cattttcagc atctccgcat cgttaccgct gtcgccaatc gctaccacat tttgcggtga
    18181 cagatcccag cgtttcagta accgcgaaat accgtttgct ttatgtagac cgggaataat
    18241 caggtcgata aagccaaaac cactggtgac gggtttcata atgccatcca gcgctatgtg
    18301 tagtttgtcg atcactaacg ggatttgttc atccggcagg ttgagcgaaa acttgaacag
    18361 tacgtcgtca atctcctgat aatcttttac aggtttcagg cgatggtagt gttttgccat
    18421 cagtgcgaca aatgcttcgg gggcattttc gctgacatat gcactttgca gaccgcaggc
    18481 gacaaaattg agttgcttat cttttagcaa ctcgccaata acaatccgcg attcatgtcg
    18541 ggtcagttcg ccgtggaaca gttgcttgcc atgttcgtaa accagtgcgc cgttttccgc
    18601 gacaaaagag atctcatcct ttagctcagg aaagaacgaa ataagctggt aatactgatt
    18661 accgctggcg acaacgaact taatgccgcg ctttttcagt tcctgatatt gcgccataaa
    18721 acgtggttgg ttgtacgttt tggcgtcgtt aagaaaagta ccgtccatat ctgtgacgat
    18781 aacttttacg ctcataggtg tgctcctggc tcgaaaatga aaccgtaaca gtgtaataac
    18841 aatgtgacgc agagcacaaa ttatatttcg aatgaaagta aggatgaaat tgatgatgtg
    18901 aatgatttag cccggcgacg ccgcagccgg gccgaagaga ttacagcata tgttcagtac
    18961 gggcgatgat atcgtcctga gcgtctggag agagcgcggt gaagaaggcg gaataacccg
    19021 cgacacgcac taccagatcg cggtactgat ccggatgttt tttcgcctcc agcagcgttt
    19081 cgcgggaaac gatgttgtac tgaatatgcc agcctttatg cacttcaaag aaggtacgca
    19141 gcaggatcat cagtttctgc ttgtcagatt cgttctccag cgttgccgga ttcagtttct
    19201 ggttgagcaa cacgccgccg agaatcgctg ccgtaggcag tttacccact gagccaatga
    19261 ccgcagtagg gccgagatgg tcagtaccag aggccggact agcgccttct gccagcgggg
    19321 tgtgggcttt acgcccgtcc ggcgttgcca tagtttgcgc gccaaacggt acgttagcgg
    19381 agatagacga cgtacccgcg taatagttgc cgccaaccgg accacgaccg tagcgtgggt
    19441 tatggtactg tttcagttcg tcgatatagg tctgataagc gcgagccagc agcgtatcga
    19501 cggtatcatc gtcgttgccg tacttcggcg caccgttaat cagccgctga cgcagttgct
    19561 catgagtcag gccatcgaag tcatccgcca gtgcggcagc cagctgttgc tgaccaatcg
    19621 cgccctgttc aaacaccagt ttcttcactg ccgccaggct gttgccgagg ttggcaatac
    19681 cgacctgcag gccagaaacc cagtcatatt tcgcgccgcc ttgcttgata cttttcgctc
    19741 gctcaataca gtcatccacc agcgccgagc agagaatatc gtgcacgttc tcttccagca
    19801 tggtgtcgac gacatattcg atttcgattg atttacgcgt gtagtaacgg atttgcgtat
    19861 cccacgcgtc catcacttca tcgaagttgt tgaagttacc tgccgacaac gctttttctt
    19921 gtggcaggaa cactttgccg ctggtggcat cacgcccgcc ttccagcgcc gccagcatca
    19981 cgcgggcgaa gttgataaag ctcatgccgg tacagcgata gccccatttg ccaccgacgg
    20041 cggtttctat acaaccaatc gctgcgtagt cataagcgtc ctgcggttca ataccgagtt
    20101 taataaattc cgggatcacg atttcgtcgt tgttgaatgc cggcatcccg aagccgcagc
    20161 ggatcacatg tacgcaggcg tcgaggaaat cgttactcat tcctgcatgg taacgcacgc
    20221 tgaggttagg ctgagtggaa cgcaggcgac cgcaggattc gaggatcgcg taagagagtg
    20281 gattcaccgc gtccattggt tgaccatcaa ccagattttg tccgccaatg gtgacgttct
    20341 gatacagcgg acttcccgca gaggcttttg agtgggagcc ggagcggatt ttgttcactt
    20401 ccagcaattt caaccagcag ctatgcagca attcgatggc gtgttcgcga tccagcgtct
    20461 ggttcagttc aacgtcgcgg cgatagtacg ggtagagata ctgatccata cgaccaaacg
    20521 acaccgagtg accgttagat tcaatctgca aaatcaactg gatgaagtaa cacagttgca
    20581 gcgcctgcca gaaagtctgc ggcggctggt gggcgataag atcgcagttt tctgccatcg
    20641 ccagcagttc atcgcgacgg ctttcgcggg tttcggtcgc ggccattgca cgcgccaggg
    20701 cagcgaaacg ttcaatgtgt tcactgactg ccaccagcac gatatcaatc gctttcagga
    20761 actgttcgcc gtgcaaatct tccagcaccg tcagattgat gcgcgagcga cgttccgcta
    20821 ctttctcacg cagaccatca agcccttttt ccagcagcag cgggaaattg accgccaggt
    20881 gcgcatcgcc ggaggtcata ttgccttccg ctttaatgat gccggtcgcc agcagacctt
    20941 tttgctcatc ggtaaacatg ccatagcagc gatcctgtac cgtctgaccg cgccaccacg
    21001 ggcacacttc atgcagaaca cgtttgttct cttcgctcac cgcaaagcca gcaccgggac
    21061 gatctgccag atcatcaatc tctttttcga tccacgagac agtatattcc gggaagatcg
    21121 gcgcggcgcg aacttcgctt gcctggttgc caatgatcaa ctcatcgtgt ttgatccaga
    21181 tggtgcgatt cgccaggtga tgcgccagtg ccagcgcgcg acgtaccggg atcggcttat
    21241 cgagatgttg ttgatacatc tcggtatagt gctgcgcgcg ctcggtacag actggcggct
    21301 tcacaatatg caccagcgca tttttgtgcg ctttaatgcg gtcgctgagc gtgtccagtt
    21361 tcagtgtggt catggttgtt atcctcgtaa ggtcgcggtt aaccctttct ggcaggcata
    21421 ctgctgggca aaatcgagca gctctggcgc atcaagcggt ttttccgggg cgtcataggg
    21481 cagattaagt aagtgatatt tgttgatgcc cagcgtgtgg tagggcagaa aatgaatttc
    21541 gccaacgtgc agctcgtcgg cggcaaaatc ggtaatggct tttacagagg tttcgtcggc
    21601 attaaagccc tgaatcagcg gcacgcggat aatgattttt ttgcccgctg cggcgagttt
    21661 tttcaggtta tccagtaccc tggcggcgtt accgtcggtc cactgtttaa acggcgcgtc
    21721 ggcaacgtgt tttaaatcgg caagaaacag atcgatatag ggcagagaag gggcgatata
    21781 tttccacggc acatgcagac aggtttctac cgcagtatga atgcctgcct cgtggcaggc
    21841 ttgcagcagc gccgtcgcca tttccggctg catgaagggt tcaccacccg aaagcgttaa
    21901 gccgccgccg ctgcgatcgt aaaacggttt atcgcgcaga acggtcgcca tgatctcctc
    21961 aacgcttttc acttcaccac acacggttaa cgcctgtgtc ggacagcagt cggttaacgc
    22021 cgtcagatgc tccggggtta acttttcccg atgaataagc aaaccattca gcgcgcgctc
    22081 aatcacttcc ggcgcggcct tagcgcacag ctcgcagcct tccagacaca gtcgtgcgtc
    22141 atacagcaga tcctgcgtac gggcgcggct ttccgggttc tgacaccagc ggcagcccag
    22201 cgaacagcct ttaagaaata cgaccgtgcg gataccgggg ccatcatggg tcgagtagcg
    22261 ctgaatattg aaaatcatag ttgcctctct gtttcgttca aacattaaaa tactttcgaa
    22321 tgaaagttag attgatgtgc gtcaactgtt cagagagttt tcccgtgata gtctacattc
    22381 agacaaaaag tacattttga ggatggttat ggaactgtat ctggatactt cagacgttgt
    22441 tgcggtgaag gcgctgtcac gtatttttcc gctggcgggt gtgaccacta acccaagcat
    22501 tatcgccgcg ggtaaaaaac cgctggatgt tgtgcttccg caacttcatg aagcgatggg
    22561 cggtcagggg cgtctgtttg cccaggtaat ggctaccact gctgaaggga tggtgaatga
    22621 cgcgcttaag ctgcgttcta ttattgcgga tatcgtggtg aaagttccgg tgacagccga
    22681 ggggctggca gcgattaaga tgttaaaagc ggaagggatt ccgacgctgg gaaccgcggt
    22741 atatggcgcg gcacaagggc tgctgtcggc gctggcaggt gcggaatatg ttgcgcctta
    22801 cgttaatcgt attgatgctc agggcggtag cggcattcag actgtgaccg acttacacca
    22861 gttattgaaa atgcatgcgc cgcaggcgaa agtgctggca gcgagtttca aaaccccgcg
    22921 tcaggcgctg gactgcttac tggccggatg tgaatcaatt actctgccac tggatgtggc
    22981 acaacagatg attagctatc cggcggtgga agccgctgtg acgaagtttg agcaggactg
    23041 gcagggagcg tttggcagaa cgtcgattta accggacgtt ctgcatcctc ataaattgct
    23101 gatgacgtgg cggagtgcac cgatgcctgc cgcgcttgga aatctgtgcc cgaaccgtag
    23161 gccagataag gcgttcacgc cgcatctggc gtctggagca aatgcctgat gcgacgcttg
    23221 tcgcgtctta tcaggcttgg aggtggcaat tactgcccgc acacctcaca ccccggattg
    23281 cgcatcagtt tcatttcgcg aaactgacag gtcatcgcat cgtacatgac gattttcccg
    23341 ctggccggtt tgccataacc tgccagcagt ttgatcgctt ccattgcctg caacgaacca
    23401 attacgccta tcaacggtgc catcacgcct gcttctacgc aggttaacgc gttctcgcca
    23461 aacaaacggc taaggcagcg atagcacggt tcgccgtcct gataagtgaa gaccgtgatt
    23521 tgcccttcca tacgaattgc cgcgccggaa accagcggca ccttcgcggc aaaacagcct
    23581 gcgttcagtt gattacgtac cgcaacgtta tccgtacagt cgagcaccag atcgtgttca
    23641 gcaatcaatg ctgcaagttc tgcgtcatct agcagtgcat tgactggcgt aatcgcgata
    23701 tgtgggttga tccgcgtcag ggcgtcacgg gcggattcca ccttcggttg cccgaccgtg
    23761 gcatcactgt gcagtgtctg gcgttgcaga ttcgagagcg aaaccgtgtc gaagtcgagc
    23821 agcgtcaggt tgccgacacc ggcgcttgcc agatactgcg aagctgcgca gccgagtcca
    23881 ccgaggccga ctaccagcac acgagaatct tttagcgcct cctgaccgtc aaaatcaaaa
    23941 ccgcgcagaa tgatttgccg gttgtagcgc agcatctcct gatcgctgag ttccgccatt
    24001 acaggcctcc gaacaacgcg ttaaacggtt ctacttccac ccattcgccc acgtccacat
    24061 tgccgcgatc gcgttccagc acgataaagc agttgccgag actaaaggag ctaaatatat
    24121 gtgaaccctg atgtccggtg gtcgtcactt caagttcacc gtcggcgttg cgttgcagca
    24181 cgccgcgctg gaaatcaaga cgtcctggcg tttttttcag gcgggacgct gtgcgtacgc
    24241 gctggcgcgc gggcaggccg ctggcggtgt tgccgcttag ttttgccagc aaaggctgta
    24301 ccagttgata gaaggtcagc gtcgctgaaa ccgggttgcc cggcaggccg cagaaccagc
    24361 tattgctgag tttaccgaac gcgaacggtt tacctggttt aatcgccagc ttccagaagg
    24421 cgatctcccc cagctcttca agaatcattt tggtgtaatc cgcttcaccc actgaaacac
    24481 cgccggaact gatcactaca tccgcctggc tgtcggcttc aataaatgcg gcgcgcaggg
    24541 catgtggatc gtcgcggata atccctaagt tgattacctc gcaacccagc tgttctaaca
    24601 tcaggtgtac ggcgagacgg ttggtgtcgt agatttggcc gtcgcccagc ggctgaccgg
    24661 gcaactggag ttcatcaccg gtagaaaaaa gcgctacacg cactttacga atcaccggaa
    24721 cttcggcaat ccccagtgaa gcaattactg gcagctctgc ggtagtcagg cgagttcccg
    24781 ccgggaaaac aaccgcacct gcagagatat cttcaccgcg acggcgaata ttttgcccgc
    24841 tacgcacttc agcagtaaaa cgcacgccat tgtccgtttg ttcagtctgc tcctgcatca
    24901 ccaccgcttc gcagccttcc ggcaccggcg caccggtcat aatacgaatg caggtacccg
    24961 caggccattc accatggtat ggctgaccgg caaaggattt accggcaacg ggcagcggtt
    25021 gcccggaggc aatatcggct aaacgcaccg cgtagccgtc catagcggag ttatcaaacc
    25081 ccgggacatc cagtggcgaa acgacatcgc tcgccagaat acgaccaaaa cactgaacca
    25141 gtggcagcgt ttcctgggtg gtcagtgggg taacgcgaga aagcatctca ttaagcgcgg
    25201 tgtcgagcga catcaatccg gtggtaaatt ccatgaaaac actcctgcgg aggcaaaatc
    25261 gaatttgcct attatgtcag aaaaacgcca tagactgtat gccacctcgg gcgtagcgtt
    25321 ggctcctgcc tttacatgcc atatccatct ttctatattc aaaaattgaa tgagtaattc
    25381 ataaaaattc tgatatttat agcaaaagtg gcgagccacc cttaatggac gaatactatg
    25441 ggcaaagcag tcattgcaat tcatggtggc gcaggtgcaa ttagccgcgc gcagatgagt
    25501 ctgcaacagg aattacgcta catcgaggcg ttgtctgcca ttgttgaaac cgggcagaaa
    25561 atgctggaag ccggcgaaag tgcgctggat gtggtgacgg aagcggtgcg tctgctggaa
    25621 gagtgtccac tgtttaacgc cggaattggc gctgtcttta cgcgtgatga aacccatgaa
    25681 ctggacgcct gtgtgatgga tggtaacacc ctgaaagccg gtgcggtggc gggcgttagt
    25741 catctgcgta atccggttct tgccgcccgg ctggtgatgg agcaaagccc gcatgtgatg
    25801 atgattggcg aaggggcaga aaattttgcg tttgctcatg gcatggagcg cgtctcaccg
    25861 gagattttct ccacgccttt gcgttatgaa caactactgg cagcgcgcga ggaaggggca
    25921 acagtcctcg accatagcgg tgcgccactg gatgaaaaac agaaaatggg caccgtgggg
    25981 gccgtggcgt tggatttaga cggcaatctg gcggcagcca cgtccacagg cggaatgacc
    26041 aataaattac ccggacgagt tggcgatagt cccttagtgg gtgccggatg ctacgccaat
    26101 aacgccagtg tggcggtttc ttgtaccggc acaggcgaag tcttcatccg cgcgctggcg
    26161 gcatatgaca tcgccgcgtt aatggattac ggcggattaa gtctcgcgga agcctgcgag
    26221 cgggtagtaa tggaaaaact ccctgcgctt ggcggtagcg gtggcttaat cgctatcgac
    26281 catgaaggga atgtcgcgct accgtttaac accgaaggaa tgtatcgcgc ctggggctac
    26341 gcaggcgata cgccaaccac cggtatctac cgtgaaaaag gggacaccgt tgccacacag
    26401 tgatgaactt gatgccggta atgtgctggc ggttgaaaat cttaatattg cctttatgca
    26461 ggaccagcag aaaatagctg cggtccgcaa tctctctttt agtctgcaac gcggtgagac
    26521 gctggcaatt gtaggcgaat ccggctccgg taagtcagtg actgcgctgg cattgatgcg
    26581 tctgttggaa caggcgggcg gtttagtaca gtgcgataaa atgctgttgc ggcggcgcag
    26641 tcgtgatgtg attgaactta gcgagcagag cgctgcacaa atgcgccatg tgcgcggtgc
    26701 ggatatggcg atgatatttc aggagccgat gacatcgctg aacccagtat ttactgtggg
    26761 tgaacagatt gccgaatcaa ttcgtctgca tcagaacgcc agtcgtgaag aagcgatggt
    26821 cgaggcgaag cggatgctgg atcaggtacg cattccggag gcacaaacca ttctttcacg
    26881 ttattcgcat caactctctg gcgggatgcg ccagcgagtg atgattgcga tggcgctgtc
    26941 atgccgcccg gcggtgctga ttgccgatga gccaaccacc gcgctggatg tcactattca
    27001 ggcgcagatc ctgcaattaa tcaaagtatt gcaaaaagag atgtcgatgg gcgttatctt
    27061 tatcactcac gatatgggcg tggtggcaga gattgccgat cgggtactgg tgatgtatca
    27121 gggcgaggcg gtggaaacgg gtaccgtcga acagattttt catgcaccgc aacatcctta
    27181 cacccgtgcg ctgttagctg ctgttccgca acttggtgcg atgaaagggt tagattatcc
    27241 ccgacgtttc ccattgatat cgcttgaaca tccagcgaaa caggaacccc ccatcgagca
    27301 gaaaacggtg gtggatggcg aacctgtttt acgggtgcgt aatcttgtca cccgtttccc
    27361 tttgcgcagc ggtttgttga atcgcgtaac gcgggaagtg catgccgttg agaaagtcag
    27421 ttttgatctc tggcctggcg aaacgctatc gctggtgggc gagtctggca gcggtaaatc
    27481 cactaccggg cgggcgttgc tgcgcctggt cgaatcgcag ggcggcgaaa ttatctttaa
    27541 cggtcagcga atcgatacct tgtcacctgg caaacttcag gcattgcgcc gggatattca
    27601 gtttattttt caggaccctt acgcttcgct ggacccacgt cagaccatcg gtgattcgat
    27661 tatcgaaccg ctgcgtgtac acggtttatt gccaggtaaa gacgcggctg cacgcgttgc
    27721 gtggttgctg gagcgcgtgg gcctgttacc tgaacatgcc tggcgttacc cgcatgagtt
    27781 ttccggcggt cagcgccagc gcatctgcat tgctcgcgcg ttggcattga atccaaaagt
    27841 gatcattgcc gacgaagccg tttcggcgct ggatgtttct attcgcgggc agattatcaa
    27901 cttgttgctc gatctccagc gtgatttcgg cattgcgtat ctgtttatct cccacgatat
    27961 ggctgtggta gagcggatta gtcatcgtgt ggcggtgatg tatctcgggc aaattgttga
    28021 aattggttca cggtgcgcgg tcttcgaaaa cccgcagcat ccttatacgc gtaaattact
    28081 ggcggcagtt ccggtcgctg aaccgtcccg acaacgaccg cagcgtgtac tgctgtcgga
    28141 cgatcttccc agcaatattc atctgcgtgg cgaagaggtg gcagccgtct cgttgcaatg
    28201 cgtcgggccg gggcattacg tcgcacaacc acaatcagaa tacgcattca tgcgtagata
    28261 acattcaggc ggagaataaa atggcaagag ctgtacaccg tagtgggtta gtggcgctgg
    28321 gcattgcgac agcgttgatg gcatcttgtg cattcgctgc caaagatgtg gtggtggcgg
    28381 taggatcgaa tttcaccacg ctcgatccgt atgacgcgaa tgacacgtta tctcaggccg
    28441 tagcgaaatc gttttaccag gggctgttcg gtctggataa agagatgaaa ctgaaaaacg
    28501 tgctggcgga gagttatacc gtttccgatg acggcattac ttacaccgtg aaattgcggg
    28561 aaggaattaa attccaggat ggcaccgatt tcaacgcagt ggcggtaaaa gcgaatctgg
    28621 accgggccag cgatccggcg aatcatctta aacgctataa cctgtataag aatattgcca
    28681 aaacggaggc gatcgatccg acaacggtaa agattaccct caaacagccg ttctcagcgt
    28741 ttattaatat tcttgcccat ccggcgaccg cgatgatttc accgacagcg ctggaaaaat
    28801 atggcaagga gattggtttt catccggtgg gaaccggacc gtatgaactg gatacctgga
    28861 atcagaccga ttttgtgaag gtgaaaaaat tcgcgggtta ctggcagcca ggattgccca
    28921 aactggacag cataacctgg cgtccggtgg cggataacaa cacccgcgcg gcaatgctgc
    28981 aaaccggtga agcgcagttt gctttcccca ttccctacga gcaggccaca ctgctggaga
    29041 aaaacaaaaa tatcgagttg atggccagtc cgtcaattat gcagcgttat atcagtatga
    29101 acgtgacgca aaagccgttc gataacccga aggtccgtga ggcgctgaat tacgccatta
    29161 accgtccggc gctggtgaaa gttgcctttg cgggctatgc aacgccagct actggtgtgg
    29221 taccgccaag tatcgcctac gcgcaaagtt ataaaccgtg gccttacgat ccagtgaaag
    29281 cgcgcgaatt actgaaagag gcgggatatc ccaacggttt cagtaccacg ctgtggtcgt
    29341 cacataacca cagcaccgcg cagaaagtgc tgcaatttac ccagcagcag ttagcgcagg
    29401 tcgggattaa agcccaggtg accgcgatgg atgccggaca gcgggcggca gaagttgaag
    29461 gtaaagggca aaaagagagt ggcgtgcgga tgttctacac tggctggtcg gcttcaaccg
    29521 gcgaagccga ctgggcacta tcgccgctgt ttgcctcgca aaactggcca ccgacgctgt
    29581 ttaataccgc gttttacagc aataaacagg tggatgactt cctggctcag gcactgaaaa
    29641 cgaatgatcc ggcggaaaag acccgcttat ataaggcggc gcaggatatc atctggcaag
    29701 aatcgccgtg gatcccgctg gtggtagaaa aactggtgtc ggcacacagt aaaaacctga
    29761 ccggtttttg gatcatgcca gataccggct tcagctttga agacgcggat ttgcaataag
    29821 caacgcaggg agtggaatgc ttaattacgt tatcaaacga ttactggggt tgattccgac
    29881 gctgtttatc gtctcggtgc tggtgttttt atttgtccac atgctgcccg gcgatccggc
    29941 gcgattgatt gccgggcccg aagctgatgc gcaggttata gaactggtgc gtcagcagct
    30001 ggggttggat cagccgctgt atcaccagtt ctggcactat atcagcaatg ctgtgcaggg
    30061 ggattttggc ctgtcgatgg tgtcgcgtcg tccggttgcc gatgagattg ccagccgctt
    30121 tatgccaacg ctgtggctga ccataaccag tatggtctgg gcggtgatat ttggtatggc
    30181 ggcgggaatt atcgccgccg tctggcgtaa tcgttggccg gatcgattga gtatgaccat
    30241 tgcggtgtcg gggatctcgt ttccggcatt tgcgctgggg atgtttttaa ttcaggtatt
    30301 ctccgttgaa ctgggctggc tgcctactgt gggagcagac agttggcagc actacatttt
    30361 accctccctg acgctcggcg cggcagtggc cgccgtgatg gcgcgcttta cccgtgcgtc
    30421 atttgtcgac gttttaagcg aagattatat gcgtaccgcg agggcgaaag gggtgagcga
    30481 aacctgggtt gtcctcaaac acgggctacg caacgcgatg atcccggtag tgaccatgat
    30541 gggcttacag tttggctttt tgctcggtgg ttctatcgtg gtggagaaag ttttcaactg
    30601 gcccggactt ggacgcttac tcgttgactc tgtagaaatg cgcgattacc cggtgattca
    30661 ggcggaaatt ctgcttttct cgctggaatt tattcttatc aacttagtgg tggatgtgct
    30721 ttacgccgcc attaacccgg ctatcaggta caagtaagga tgcgactatt taactggcga
    30781 cgtcaggcgg tgttaaacgc catgccactg gtcaaacctg accaggtacg tacaccgtgg
    30841 catgaattct ggcgacgatt tcgccgtcag catatggcga tgaccgccgc attattcgtt
    30901 attttattga ttgtggtggc catttttgca cgctggatcg ctccctatga cgccgaaaat
    30961 tattttgatt atgacaatct gaataacgga ccttctttgc agcactggtt tggcgtcgat
    31021 tcactggggc gcgacatttt cagccgtgtc ctggttggtg cgcaaatctc gctggcggcg
    31081 ggcgtgtttg ccgtgtttat cggtgtggcg atagggacgt tgctgggctt gttggctgga
    31141 tattatgaag gctggtggga tcggctgatc atgcgcattt gcgatgtgct gtttgccttc
    31201 ccgggtattt tactggcgat cgctgttgtt gcggtgttgg gaagcggcat tgctaacgtg
    31261 attatcgcag tcgccatttt ttccatcccc gcgtttgccc gcctggtgcg cggcaacacg
    31321 ctggtgttga aacagcaaac ctttattgag tcagcacgca gtattggtgc cagcgatatg
    31381 accattttat tgcgtcatat cctgcctggg accgtctctt ctatcgtggt gtttttcacc
    31441 atgcgcattg gcacctcgat tatctctgcc gccagcctgt catttctcgg cctcggtgcg
    31501 cagccgccga caccagagtg gggagcaatg ctcaatgagg cacgagcgga tatggttatc
    31561 gcgccgcatg tcgctgtttt tccggccctg gctatttttc tgaccgtact ggcgttcaat
    31621 ttgttgggcg atggtttacg cgatgcgctg gatccgaaaa ttaaaggata gttacgtttg
    31681 aatattgctt gaaagggtaa tcacctcaca ggaaattatt gccctaagca agtgttgtaa
    31741 ctttctgctg attttgtaga atcgggtaat ttggttaaaa agccgcagca agggactatt
    31801 tttgcagcgg cacagcgttc agatagttat tttgttaaat gtattaacat gctgagttta
    31861 tacgaaaaga taaagataag gctgataatt ttatttttat tggcagcact gtcatttatt
    31921 ggtctttttt tcatgattaa ctatcaactg gtatcggaac gcgcggtaaa acgtgccgat
    31981 agccgctttg aacttattca gaaaaacgtt ggctatttct ttaaagatat tgaacgttcg
    32041 gccctgacat taaaggactc actgtattta ttaaaaaata cagaggagat tcaacgcgcc
    32101 gtgattctga aaatggaaat gatgccattt ttagattcgg tgggactggt acttgatgat
    32161 aataaatatt atctcttttc gcggagggcg aatgataaaa tcgttgttta tcatcaggaa
    32221 caagtaaatg gaccgcttgt cgacgagtca gggcgggtta tttttgccga ttttaaccca
    32281 tcgaaacgac cgtggtcggt ggcttcagat gactctaaca acagctggaa tccggcatac
    32341 aattgctttg atcgtccggg taaaaaatgt atctctttta cgctacgtat caacggcaaa
    32401 gatcacgatt tgttagcggt agataaaatt catgtcgatt taaactggcg gtatctgaac
    32461 gagtatcttg atcaaatcag cgctaatgat gaagttctat ttttgaaaca aggtcatgag
    32521 atcattgcca agaatcaact cgctcgtgaa aaactgatta tttataatag cgaaggtaat
    32581 tataatatta ttgattctgt cgatacggag tatattgcaa agacatcagc agtgccaaat
    32641 aatgcattgt tcgaaatcta tttttattat cctggcggta atttattgaa cgcatcagat
    32701 aaactttttt atctaccgtt tgcgttcatt attatcgtgt tgctggtggt atatttcatg
    32761 accactcgtg tgttccgtcg gcaattttct gaaatgaccg agctggttaa tacgctggcg
    32821 tttttgcccg actcaacgga tcagatcgag gctctgaaaa ttcgcgaagg cgatgcgaaa
    32881 gagattatca gcatcaaaaa ttcgatcgcg gaaatgaaag atgccgaaat tgaacggtca
    32941 aataaattgc tctcactgat ctcttacgat caggaaagcg gttttattaa aaatatggcg
    33001 attattgagt ccaacaataa tcagtatctg gctgtgggga tcatcaaact gtgtggtctg
    33061 gaagccgtgg aggcggtgtt tggtgttgat gaacgcaata aaatcgtcag gaaattgtgt
    33121 cagcgaattg ccgagaaata tgcgcaatgc tgcgatatcg tgacattcaa tgccgatctc
    33181 tatttacttc tgtgtcggga aaatgtacag acatttaccc gtaaaatagc gatggtaaac
    33241 gattttgaca gcagctttgg ctaccgcaat ctgcgcatcc ataagtctgc catttgtgaa
    33301 cctttgcagg gggaaaacgc ctggagttac gcagaaaaac tgaaactggc gatttccagt
    33361 atccgtgacc atatgttctc cgagtttatt ttctgtgatg acgcgaaact caacgaaata
    33421 gaagagaata tctggattgc gcgtaatatt cgccatgcaa tggaaattgg cgaactattc
    33481 ctcgtctatc aaccgatcgt tgatattaac acccgcgcca ttctgagcgc ggaggcgttg
    33541 tgccgttggg tttctgcgga gcgggggatc atttcaccgc tgaagttcat taccattgct
    33601 gaagatatcg ggtttatcaa tgagctgggt tatcagatta ttaaaacggc aatgggtgaa
    33661 ttcagacatt ttagtcaacg tgcgtcgctg aaggatgatt tcttactgca tattaatgtt
    33721 tcgccctggc agttaaacga accgcacttt catgagcgtt ttaccaccat catgaaagaa
    33781 aatggcctga aggcgaacag cctctgtgtt gagatcactg aaaccgtgat cgagcgaatt
    33841 aatgaacatt tttatctcaa tattgaacaa ctgcgtaaac aaggggtacg gatatcgatt
    33901 gatgactttg gcaccggttt gtcaaacctg aaacgttttt atgaaattaa tccagacagc
    33961 ataaaggtgg actcgcaatt taccggcgat attttcggta ctgcgggaaa aattgtgcgc
    34021 attattttcg atctggcacg ctataaccgg atcccggtga ttgcggaagg cgtagagagc
    34081 gaagacgttg cgcgcgaatt aattaaatta ggatgtgttc aggctcaggg gtatctgtac
    34141 cagaaaccca tgccattctc cgcttgggat aaaagtggaa aattagtaaa agagtagttt
    34201 acgtatgtcc agaatcaata agttcgtact tacagtcagt ctgctgattt ttatcatgat
    34261 ttcagcagtt gcctgcggga tctacactca aatggtaaag gaacgggtgt atagcctgaa
    34321 acagtccgtt attgatactg cttttgcggt ggcaaatatt gctgaatatc ggcgtagcgt
    34381 ggcaattgat cttatcaaca cgctaaatcc cacggaggaa cagctgttgg ttggtttgcg
    34441 cacagcttac gccgactcgg tttccccctc ttatttgtac gatgtcggtc cttatctgat
    34501 ttccagtgac gaatgtattc aggtaaagga gttcgagaaa aattattgtg cagatattat
    34561 gcaggttgtg aagtatcgac atgtcaaaaa tacagggttt atctcttttg acggtaaaac
    34621 cttcgtctat tacctctatc cggtaactca caatcgtagt ctgatatttt tgcttggtct
    34681 ggagcgtttt tctttactgt caaaatcgct ggtgatggac agcgagaacc tgatgttctc
    34741 tctatttaag aacggtaaac cggtgaccgg tgatgaatat aatgctaaaa acgccatctt
    34801 caccgtttcg gaagcgatgg agcacttcgc ctatttgccg accggattgt acgtatttgc
    34861 gtataaaaaa gatgtttatt tgcgggtttg tacattgatt attttctttg ccgcattggt
    34921 ggcagtgata ttgggtgcca gttgcctcta tctggtacgc agagtgatta atcgtggtat
    34981 tgtggagaaa gaagccatca ttaataacca ttttgaacgc gtactggatg gcgggctttt
    35041 cttttcggct gccgatgtca aaaaactcta cagtatgtat aactcggcgt tcctggacga
    35101 cctgaccaaa gcaatgggca gaaaatcctt tgatgaagat ttaaaagcgc tgccggaaaa
    35161 aggcggttat ttgtgtctgt ttgacgtcga taaattcaaa aacattaacg acaccttcgg
    35221 tcatttgctg ggcgatgaag tgttgatgaa agtggtgaaa atccttaaat cacagatccc
    35281 ggtagataaa ggtaaagtct accgcttcgg aggtgacgaa tttgcggtga tttatactgg
    35341 cggcacgctg gaagagttgc tatcgatact aaaagaaatc gttcatttcc aggtgggaag
    35401 cattaattta agcaccagta tcggtgtagc gcattcaaat gaatgtacta ccgtcgaacg
    35461 cttgaaaatg ctggcggatg agcggctgta taagagtaag aaaaacggca gggcacagat
    35521 tagctggcag taatcattat tcgcaggccg gacaaatgat tttgcccggc ctgaattaat
    35581 taaacccggc taccccacaa atcgtactca tcggcgtgct cgactttcac acgcaggata
    35641 tcacccggct taacgttggt ttcaccattg agataaaccg cgccgtcgat ttccggtgca
    35701 tctgccatgc tgcgaccaat cgcgccttct tcgtccactt cgtcgataat caccagaatt
    35761 tcacggccca ctttctcttg caggcgctcg gcggaaatct gctgctgcag ctgcatgaaa
    35821 cggttccagc gttcttcttt cacttcttcc ggaacctggt caggcagggc attggcgtct
    35881 gcaccttcaa ccgggctgta tttaaagcag ccaacgcgat ccagacgtgc ttctttcagg
    35941 aagtcgagta gcatctggaa atcttcttcc gtctcgccag ggaagccgac aataaaggtt
    36001 gagcgtaggg tcagttccgg gcagatttcg cgccactgtt tgatgcgcgc cagttggcga
    36061 tctacagaac ccggacgctt catcagtttg agaatgcgcg ggctggcgtg ctgcaacgga
    36121 atgtccagat acggcaggat tttgccttct gccatcagtg ggatgacgtc gtccacatgc
    36181 ggataagggt aaacgtagtg cagacgtgtc cagatcccca gtttcgataa ctgttcgcac
    36241 aggctgacca tgctggtttt taccggctcg ccgttgtgga agccagtacg atgtttaaca
    36301 tcaacgccat aggcggaagt atcctgcgag atcaccagaa tctctttaac gcccgcatct
    36361 accagacgtt tcgcttcact taacacttcg ccaatcggac ggctcaccag gtcgccgcgc
    36421 atagacggaa taatgcagaa ggtgcagcgg tgattacagc cttccgaaat tttcagatag
    36481 gcataatgac gcggcgtcag tttcacacct tgttctggca ctaggctcag gaatgggttg
    36541 tgtttcggtt ttggcacgta gtgatgaacg tgctccagaa cctgctcata gctatgaggc
    36601 ccggtgattt ccagcacttt cgggtggact tcgcggatct gatcttcttt tgcccccaga
    36661 caaccggtca caattacctt gccgttttca ttcaacgctt caccaatggc ttccagtgat
    36721 tcttgtaccg cgctgtcaat aaagccgcag gtgttgacga tcaccatgtt cgcatcgtca
    36781 tagctcggta ccacgtcata accttcagtg cggagttcgg tgagaatacg ctctgaatcg
    36841 acaaggtttt tcggacagcc aagggaaaca aagccgattt tcggctgggg agttactttg
    36901 ctcatagctt aaaaaatatt cagttacagg aaaggtcagg gcagggattc tacagagttc
    36961 tggataaaat ttgtatcgca atctcattcg ctggcggagg cgaaggaaat gtaaattttg
    37021 ttaattcggc gtgaagaatt gatcctggac agcattttgc tcaaaaaata gccatactat
    37081 ttaattgcaa caaggctgga aagaggagga tcgaagtatg ttcgttgaca gacagcgaat
    37141 cgatctgctg aaccggttga tcgacgcacg cgttgacctc gctgcatacg tgcaactgag
    37201 gaaggcaaaa ggatacatgt ccgtcagcga aagcaatcat ctacgagata acttttttaa
    37261 actgaatcgc gaactgcacg ataaatcgct gcggttgaat cttcatctgg atcaggaaga
    37321 gtggagtgct cttcatcatg ctgaagaagc attagcgaca gccgcagtat gtttgatgag
    37381 tgggcaccat gattgcccga ctgttattac cgtcaacgcc gataagcttg aaaattgtct
    37441 gatgagctta acgctgagta tccagagcct gcagaagcac gccatgcttg agaaggcctg
    37501 aaaactaagg gggagaaagc gtctccccct tcatgtttaa gtttttgtaa aaatgaattt
    37561 gttatctcct ccactgacta cgctttaagc cagagtcaat ccggaggcgt tatgcatcga
    37621 caatcctttt tccttgtgcc ccttatttgt ctttcttccg ctctctgggc ggctcctgca
    37681 acggtaaatg tcgaagtact gcaagacaaa ctcgaccatc cctgggcact ggccttttta
    37741 cccgataatc acggtatgtt aatcactctg cgcggcggcg agttgcgtca ctggcaagca
    37801 ggaaaaggat tatctgcgcc gctttccgga gttccggacg tttgggcgca cgggcagggc
    37861 ggcctgctgg acgtggtttt agcgcctgat tttgctcagt ctcgccgcat ctggttaagt
    37921 tattccgaag ttggcgatga tggcaaagcc ggaactgctg tgggttatgg ccgcttaagt
    37981 gatgatctct caaaagtgac cgacttccgc accgtcttcc gccagatgcc aaaactgtct
    38041 accggcaacc attttggcgg gcggctggta ttcgacggta aaggttatct ttttattgct
    38101 ctgggcgaaa acaatcagcg cccgacggcg caggatctgg ataaattaca gggcaaactg
    38161 gtgcgtctga ccgaccaggg cgaaatcccg gatgataatc cttttataaa ggaatctggt
    38221 gcgcgcgccg agatctggtc ttatggcatt cgtaatccgc aaggaatggc gatgaatccg
    38281 tggagtaatg cactgtggct gaatgaacat ggcccgcgcg gtggtgatga aattaatatc
    38341 ccgcaaaaag gcaaaaacta cggctggccg ctggcaacct ggggaatcaa ctattcaggc
    38401 tttaagatac cggaagcgaa aggggagatc gtcgccggga ccgagcaacc tgttttttac
    38461 tggaaagatt cgcccgctgt gagcggcatg gccttctata acagcgataa attcccccag
    38521 tggcagcaaa aattatttat tgggagctga aagataaaga tgtcattgtg atgagcgtca
    38581 acggcgacaa agtgacagaa gatggccgta ttttaacgga cagagggcag cgaattcgtg
    38641 atgttcgcac tggacccgac ggttatttat acgttctcac cgacgagtcc agtggggaat
    38701 tacttaaagt tagcccacgc aattagctaa cgggaatcat caccactttg cgcactgcgg
    38761 ggcgttcagt gagttgctga taccagcgtt gcagatttgg acgcggtgtc caggtcaggc
    38821 caacgttaaa caaattgtag atgaacggtg cgatagcgat atcgcccaca ccaaactcgt
    38881 cgccagagaa ccattttact tttgccagtt ccgcatcgag cagggcaaac agggcgtcgc
    38941 actctttgca actggcatca atggcggcct gatcgcgctc ttccggtggt gttctgacta
    39001 atcccatcag gatcccgcga tgagcattac tgagcgtctg gtttgcccag tccatccatt
    39061 tttccgcttc cgcacgacgt gccggtgagt cgatccacag gcgtttttgc ccgtactgtg
    39121 ccgccagata gcggacaatg gcgtttgatt cccaaagaat gagatcactt tcgtcgtcac
    39181 gcaacaacgg caccagcccg ttagggttca tcgccagaaa atcagcatcg tgatttatcc
    39241 caaactcacg gcccgcgaga atttgctcat aaggtagttc cagttcttcg agcgtcagca
    39301 atactttttt tacgttagtt gaattattcc gaccccacag cgtaatcata ctcacccctt
    39361 ttccgacagg cagccacaga ctgaacagga ctccgatggt gaagtaaagt taacttttaa
    39421 gcaacagctg gcaaaaaaat gacaccagaa gcagcgaagc ggcaggatat tgcataaact
    39481 ttaaaaactt taccaactta cggtttcttt aagtttgtgt gtgcgttatt aatcaccgaa
    39541 cttatcatac ggcgatataa cgtatttttt tgaatggata ctcgggtggc atttatgacg
    39601 caatactcct ctctccttcg tggtcttgca gcgggttctg catttttatt cctttttgcc
    39661 ccaacggcat tcgcggccga acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg
    39721 attttaatgg attacgccag cggtaaagtg ctggcagaag gcaatgcgga tgagaaactg
    39781 gatcctgcga gcctgactaa aatcatgacc agctatgtgg ttgggcaggc gcttaaggcc
    39841 gataagatta aactcaccga tatggtgacg gtcggtaaag atgcctgggc gacgggaaat
    39901 ccggcactgc gtggttcatc ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca
    39961 gacttgaaca aaggtgtgat tatccagtcc ggtaatgacg cctgtattgc gctggcagat
    40021 tacgttgccg ggagccagga gtcctttatt ggtctgatga atggttatgc caaaaaactg
    40081 gggctgacca acactacctt ccagacggtg cacggcctgg atgcgccggg gcagttcagt
    40141 accgcgcgcg atatggcatt gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac
    40201 gccattcata aagagaaaga gttcaccttc aacaaaattc gtcagcctaa ccgtaaccgt
    40261 ctgctgtgga gcagtaatct gaatgttgat ggcatgaaga caggaaccac ggcaggtgcg
    40321 ggatataatc tggttgcttc ggctacccag ggtgatatgc gtttaatctc cgtagtgctg
    40381 ggggcgaaaa ctgaccgtat ccgttttaat gagtctgaga aattattgac ctggggtttc
    40441 cgcttctttg aaactgtgac gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc
    40501 tggtttggtg ataagatcga agtgaatctc ggggcaggcg aagcgggctc cgtgaccata
    40561 ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc gcagcttacc
    40621 gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa cggtaaatcc
    40681 attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtagg
    40741 atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct
    40801 tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa
    40861 tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca ctcaccacaa
    40921 tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat ttactgtggt
    40981 cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc ggcaactctt
    41041 caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa taaaaagcga
    41101 tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa atggcgttgc
    41161 tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct ttcagcgcca
    41221 gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca atcgcttcaa
    41281 taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg
    41341 tcgcagccag ttttgccgcc cggcgttttt cttccaccaa gcgggatttt tgatcgctta
    41401 acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc aaaacgacgg
    41461 gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg
    41521 cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc tgcccgatac
    41581 gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag ccgctcaata
    41641 ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt aatgcaaaac
    41701 agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct tgccagataa
    41761 tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata tccagcggcc
    41821 agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc attaaaagtg
    41881 agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac
    41941 cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag ccgatatttt
    42001 cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca aacaggctga
    42061 ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc ccccacaacc
    42121 aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata aaaatcgcca
    42181 acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat agagagagat
    42241 tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca atacaggcaa
    42301 gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta tttccctcaa
    42361 gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact taaagaaagc
    42421 gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg
    42481 ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg aagaaattgc
    42541 atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact tttccctctc
    42601 tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca acccggtatg
    42661 ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc gatgaccgcg
    42721 tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga tcgtattggt
    42781 cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct ggcaatatta
    42841 ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat aagcttctgt
    42901 ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc
    42961 aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg tccgctggtg
    43021 ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt tgccgcattg
    43081 gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat gcgtataggc
    43141 gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa gaacggccgc
    43201 tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat cgcccagtcg
    43261 ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt gctgcaagtg
    43321 cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac ctcgcgccgc
    43381 accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct gttggtcgct
    43441 gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg gttaagtatt
    43501 tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct gtttgccagc
    43561 gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct gatctttacc
    43621 gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact gtttaatctc
    43681 ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt aaaagataaa
    43741 cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc gatcaggcat
    43801 tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct tcacctacgc
    43861 tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca ggagccacat
    43921 agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact gaatcaggaa
    43981 ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc atcgccacac
    44041 cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc atttcgcctg
    44101 aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc attgtgttgc
    44161 cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac tttatcgatc
    44221 acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc tgcgacgact
    44281 gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat ctcaatatcg
    44341 ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa tttctgcagt
    44401 tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct gccgttgccg
    44461 gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg tacttgtgga
    44521 atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa gttgtcaaag
    44581 ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat ggcatcgtca
    44641 tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac ttccgggcgc
    44701 gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc attaaaaaca
    44761 tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat ttcattcgca
    44821 atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac cacaaagcga
    44881 atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt ataggttttt
    44941 tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct catcaactat
    45001 tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg acaatagccg
    45061 cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa tgttcgccga
    45121 gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt gctaccacac
    45181 tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc agcgggaagc
    45241 ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag gcgctatcga
    45301 caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga accacggcaa
    45361 cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc atccccaggg
    45421 taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca tcaaccatta
    45481 ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc actacaacac
    45541 caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca tgctgggtgc
    45601 catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata taaataggcg
    45661 cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca aaggccgtca
    45721 gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg tgcatcatcg
    45781 gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt atcgccactt
    45841 ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc aggggcgatg
    45901 tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac atcgtgacca
    45961 ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga ataccgctca
    46021 tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag acagagagaa
    46081 tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct ggcaaaaaga
    46141 agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa tttacggtca
    46201 tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt acatttgtac
    46261 acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg gcgagaaaaa
    46321 attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt tacgcaccgc
    46381 aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt ttcaggaatt
    46441 gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg gcaatatcag
    46501 gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac cgatatgatc
    46561 tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct ctacgcgctg
    46621 gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg cagtcagcaa
    46681 acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt tattgagggg
    46741 atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat tttgaggatg
    46801 gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca tcaaccttga
    46861 accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa agcagtggca
    46921 ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa tttcattttc
    46981 agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg tccctgccag
    47041 cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag ggatgttact
    47101 gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc ccatcattgc
    47161 gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga aacagataac
    47221 cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa tcgcgcccag
    47281 gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca ccatcaagcc
    47341 gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg ggtggttagc
    47401 acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc ccatgccgaa
    47461 actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa caaagaaggc
    47521 gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga tgcgatcggc
    47581 gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt tgttaagcac
    47641 gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa ggaagcaacc
    47701 gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt ttttaacgac
    47761 cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt ctttaccgtt
    47821 acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg ctatttcatc
    47881 gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac gtcgaatacg
    47941 ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt tgccgtcggt
    48001 ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat aaaccttacg
    48061 gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg tctgtaagtc
    48121 ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca gaccgattaa
    48181 ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct gcctgctttc
    48241 catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg acgtcataga
    48301 gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac ctaaccctaa
    48361 ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat ttttcccatc
    48421 acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga aaataaacag
    48481 cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc ctaataacag
    48541 cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc gtaactttcc
    48601 gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc catttaacaa
    48661 ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc tgttgaaaga
    48721 aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca ttcagcgcac
    48781 ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg tatcaacggc
    48841 tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat cttactgaac
    48901 gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga gtgaaacata
    48961 aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg tgcctttttc
    49021 tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc ggcttgctgt
    49081 ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa gtcctgaaac
    49141 ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg ctgttttttt
    49201 caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg ttctggtgtg
    49261 cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag cacagaaaaa
    49321 atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga ttttctttca
    49381 cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa atctgcggca
    49441 cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc ccttccgcac
    49501 gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc tcagccagat
    49561 cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt tgcattattt
    49621 ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct gaaagtaggt
    49681 ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca gtgaaaagtt
    49741 ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta aattgcctaa
    49801 aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc tgctgtcggt
    49861 aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga ttctgatgat
    49921 ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc aggtggcaaa
    49981 acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag aaagagaaaa
    50041 agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc catttcactg
    50101 atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt gcgacgtcca
    50161 gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca ttacgtgaag
    50221 aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag ttctgggtgt
    50281 tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc ggcctggcgg
    50341 aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca ttaaccgcag
    50401 cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat attgaagcgg
    50461 tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg tgccttggct
    50521 ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg gtgcatgaaa
    50581 acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa ctggcggaat
    50641 attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat atccgccgaa
    50701 caatcattaa agaaagccgc ccatttattc tggatttttt gcacaaacag ggttgggcga
    50761 cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt ctgacagaga
    50821 ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg tgtaagcggc
    50881 tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg ctttcttgct
    50941 acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag ttaccccatt
    51001 ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg gcggcactgc
    51061 gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc
    51121 gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg cctgtcacgg
    51181 gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt ggtgcgccgc
    51241 tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg gagactcgtc
    51301 aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg
    51361 aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt gatgaagtgg
    51421 tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg catcgtggcg
    51481 gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata aaagccgcgc
    51541 gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc gggccgttgg
    51601 tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc ggtatcgata
    51661 tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat tgcctgaaaa
    51721 cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt ttgcgtaagc
    51781 tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg gtcgttcctg
    51841 gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt gaatgtgata
    51901 actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt gatgccaaaa
    51961 tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga ccttcagccg
    52021 tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat
    52081 ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct ttatccgtta
    52141 tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt
    52201 cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac gatgaagggc
    52261 aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc acgcagtcgc
    52321 tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc ttaaagatta
    52381 tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca taaaaatttg
    52441 ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt tattcttgcg
    52501 atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt acaccgcgcc
    52561 gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt gttcgtttgt
    52621 taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg ctatcgggtc
    52681 tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa caaaaaacac
    52741 tccacattta taactggtct gattatatcg cccctgacac ggtggccaat tttgaaaaag
    52801 aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg gaaggcaaat
    52861 taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt ctagaacgcc
    52921 agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag tggaagaatc
    52981 tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa tttgctatgc
    53041 cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa gctgtgctgg
    53101 gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat ctggaaaaac
    53161 tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct accgtgttga
    53221 attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga ccggcaaccg
    53281 atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa tacattaacg
    53341 acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc tggcaggcgt
    53401 caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt ccaaaagaag
    53461 gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac aaagacgaag
    53521 cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt tccgaccatg
    53581 tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa gtccgtgaga
    53641 acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg aaagtgcagg
    53701 acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc ggaaaataat
    53761 ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca ccatcatggt
    53821 gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc tcgcccgcag
    53881 gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac caaatcctac
    53941 gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga aatcttcgcg
    54001 ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc aggtttcgaa
    54061 caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt tccgccttac
    54121 ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat gaccgtggaa
    54181 cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat ttccagtcgg
    54241 gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa accgcatcag
    54301 ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa gcgcccgaaa
    54361 ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga ccggatgcag
    54421 cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt cacccacgat
    54481 caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg gaaatttgtc
    54541 cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag cgccgaattt
    54601 attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga tggcctggtg
    54661 cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc ggtggtcgat
    54721 aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga agagccgccc
    54781 gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct cggcgatctt
    54841 tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct acaaaacgcc
    54901 catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg ctgggaagtg
    54961 gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga acctgctgcc
    55021 cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat gaagcatggg
    55081 cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct gctgccattt
    55141 ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc ttataccgaa
    55201 ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa ttttctgcaa
    55261 ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt ggctgcgatt
    55321 tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc gcacagtaag
    55381 ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac ctcgtttctg
    55441 atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa taattttctg
    55501 ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct ggcagtttat
    55561 attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac cgcgttgatt
    55621 cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc gctgaaaacg
    55681 ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc gatgctggtg
    55741 tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc ggacagcatc
    55801 atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc ggtggcctcg
    55861 gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt ccacaaacac
    55921 cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt tcgccctggc
    55981 ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg atgctggtta
    56041 tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca acgcgctggt
    56101 atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc ctgacaattg
    56161 ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg gtgctggtgc
    56221 gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg ccgctggtaa
    56281 tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt gctcatgcca
    56341 ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc actttctgta
    56401 cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc tcgatagaag
    56461 aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt acgctaccga
    56521 tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg cttgatgatc
    56581 tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg ctggtctttt
    56641 ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg atcctcggcg
    56701 cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa aaacagcgga
    56761 tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca acctggctac
    56821 ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg gattttttaa
    56881 gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg tggcggcgct
    56941 cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc tgggcgtgcg
    57001 cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc tggtcttttt
    57061 aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg tgaaagggcg
    57121 tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt acttgtgggc
    57181 ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat caaaacgtga
    57241 aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga tgctgctgtt
    57301 cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat cgtcgcccct
    57361 gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc tgtcgttcct
    57421 gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc gatcttaaaa
    57481 atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc ccggaacaag
    57541 ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca ctgctcggta
    57601 tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat ccagcctcat
    57661 ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta acgccctaca
    57721 acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc cagagtgatg
    57781 gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa ctgcgtaagg
    57841 cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc aatattcagc
    57901 cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa caacaggcac
    57961 tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc ttccagacta
    58021 atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga cagctgccgg
    58081 ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac tgcgcgacgc
    58141 ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt gcaaagcagt
    58201 cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc actcagtttg
    58261 ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc cgcggcattg
    58321 gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc tactccagct
    58381 gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc atcgaacggg
    58441 tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg ctgctggtga
    58501 agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct ttgttcatgc
    58561 cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa tacattgcag
    58621 agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt catcagtctc
    58681 aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc tttaattgct
    58741 gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac agcgataccc
    58801 aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc cagttgcgga
    58861 ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc aatacgacca
    58921 tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt cacttccggg
    58981 tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat gcgtttgcct
    59041 ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc tgagttttca
    59101 tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc cataccggag
    59161 attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa cgcgtgatta
    59221 gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc gatatcaaag
    59281 ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc tgaaacgcca
    59341 aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa agcggcaaga
    59401 actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg ccgtgtctgc
    59461 ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat taaataattt
    59521 aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta taagaattta
    59581 tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac cgtgctgatg
    59641 tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga atgtacgcag
    59701 ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg catcatcagc
    59761 gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac cattacatcg
    59821 taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa
    59881 gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt
    59941 ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta
    60001 caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta
    60061 tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc tgacaacaaa
    60121 tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta ataaatcagg
    60181 aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac
    60241 accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc
    60301 acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat cagctcgggt
    60361 aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt
    60421 tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc
    60481 aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt aaatcattca
    60541 cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc
    60601 caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg
    60661 acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc aacgcgccgc
    60721 gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga
    60781 acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg aacccaagat
    60841 tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa tagataaaca
    60901 gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg
    60961 cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc gccagcccga
    61021 caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa
    61081 attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat
    61141 ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg ttgccctgac
    61201 gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta tcgcccactg
    61261 ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca ccaaatacgc
    61321 tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg
    61381 tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg ttctgaacgc
    61441 cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca
    61501 gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc tccggagtga
    61561 tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg atcaggctgt
    61621 caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt gcctgtgcca
    61681 ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg
    61741 gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa
    61801 ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata
    61861 gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat
    61921 atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg
    61981 ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg
    62041 tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc
    62101 catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg
    62161 gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag
    62221 cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac
    62281 ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc
    62341 gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag
    62401 cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc
    62461 gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa
    62521 cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac
    62581 tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt
    62641 atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca
    62701 aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga aatgccggat
    62761 aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg
    62821 tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg acccaacact
    62881 ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt ttgagatcca
    62941 cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg gcaacgtggg
    63001 cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat tcacgatgac
    63061 tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa taggggcgca
    63121 gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt tgagccacac
    63181 tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta taagctggcg
    63241 tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat agtgaacggt
    63301 agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc aaacgctatc
    63361 agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac ctgattgggt
    63421 ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact taaattaccc
    63481 aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct gaaatgatca
    63541 tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag aggttaagag
    63601 catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg gttattgtct
    63661 aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg attgccaaaa
    63721 ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac tctcgataat
    63781 attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag tgaagaataa
    63841 attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg tgcttttata
    63901 attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt ttaatatgat
    63961 aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc aaagtgataa
    64021 tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta agggtgagtt
    64081 gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg acagtgatct
    64141 cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc aatttaatac
    64201 attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat cagaagataa
    64261 cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta atgtatcgaa
    64321 tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata aattacaaga
    64381 acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt ataccattgg
    64441 tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact ttaacaaaga
    64501 acaatatata tcctttataa aggacgcatt tattcatttt tatgattata gcaggcgata
    64561 tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat tgatgagcac
    64621 tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta cagaagaaga
    64681 gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg ctgatgataa
    64741 ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg cctttcaaga
    64801 taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata tttgtagcgt
    64861 aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga atgaagaagt
    64921 tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa gtaatttagt
    64981 caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt ttaatgacgt
    65041 ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg tgaatgaaag
    65101 catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa taacaaatag
    65161 caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa ttaataacgt
    65221 aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata tgataagagt
    65281 taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga taaaaccctg
    65341 gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct gtttactatc
    65401 tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata acgtgaaggt
    65461 tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt gtattaaact
    65521 attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct tcgcttatat
    65581 taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg accaaaaagc
    65641 aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc atttacaata
    65701 taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac aggtaagcgg
    65761 taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga cttaagtggg
    65821 tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc caacatggca
    65881 tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat cgatcatgca
    65941 attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg gtgtggtgac
    66001 cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta tccgcgatat
    66061 cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt
    66121 tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg gtattgatat
    66181 cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc
    66241 ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct gttactggca
    66301 gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacac
    66361 caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat
    66421 tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta tctgatccct
    66481 gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa
    66541 ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct
    66601 attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa atattttgcc
    66661 ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat tattgcccgt
    66721 tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac
    66781 gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg
    66841 gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc
    66901 gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga tatgacaccg
    66961 cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac
    67021 ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa atacgggcag
    67081 gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gatgccttca
    67141 tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta
    67201 aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt tgctgacttg
    67261 cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg gacggaacga
    67321 atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga
    67381 tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca ggcttcctgg
    67441 cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac cagcgcactg
    67501 ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag acggggaata
    67561 aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt
    67621 ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct gcgggcaaac
    67681 tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg gtgataatcg
    67741 aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc gacagcccat
    67801 tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg tgtcccccag
    67861 ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat cactttagct
    67921 tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat tttttccagc
    67981 aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt ctggcataaa
    68041 aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac cttcatatca
    68101 atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc atgatgcaat
    68161 gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt tctgccagtc
    68221 tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc atcaataacc
    68281 agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg atagtacgat
    68341 agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct ttcatgccaa
    68401 ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta tccaccgcat
    68461 cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga ccgatcgcgc
    68521 ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc tctttaccgc
    68581 cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta acggtaaacc
    68641 ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt
    68701 agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta cgtaccgcgt
    68761 catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca tctgccagca
    68821 gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg gaagtaatca
    68881 cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc aaccagcggc
    68941 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
    69001 cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc accagatagt
    69061 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
    69121 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
    69181 cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga atgtccgccg
    69241 tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
    69301 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccagct
    69361 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
    69421 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
    69481 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgctagga
    69541 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
    69601 cgagaactaa aatgcgttgc ggcacgtatc tctccttaac gcgccaggaa tgcgcgccag
    69661 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
    69721 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
    69781 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
    69841 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
    69901 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
    69961 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
    70021 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
    70081 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
    70141 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
    70201 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
    70261 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
    70321 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
    70381 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
    70441 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
    70501 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
    70561 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
    70621 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
    70681 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
    70741 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat
    70801 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
    70861 agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc gcacgcttcg
    70921 gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta acctccggcc
    70981 ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc
    71041 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
    71101 cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc ctgcggcatg
    71161 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc
    71221 agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat ggcgtcatcg
    71281 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
    71341 ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga
    71401 aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt tgatttgata
    71461 tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg attaatatca
    71521 atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt
    71581 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc gattaacccg
    71641 gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg tagggcatga
    71701 acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
    71761 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
    71821 aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg cgtggttgcc
    71881 ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga aacgccacgg
    71941 ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc cgggctggaa
    72001 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
    72061 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtta
    72121 ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc gcacgatccg
    72181 gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact
    72241 tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcgctaaga
    72301 ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt
    72361 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
    72421 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
    72481 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
    72541 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
    72601 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
    72661 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
    72721 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
    72781 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
    72841 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
    72901 acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag acgaccagcg
    72961 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
    73021 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
    73081 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
    73141 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
    73201 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
    73261 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
    73321 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
    73381 cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
    73441 gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
    73501 ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
    73561 cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
    73621 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca atcgctttct
    73681 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct
    73741 ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga cctgcatcta
    73801 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
    73861 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
    73921 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
    73981 ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga ccaaagccca
    74041 cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
    74101 tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc
    74161 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
    74221 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
    74281 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
    74341 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
    74401 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct
    74461 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
    74521 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
    74581 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
    74641 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
    74701 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
    74761 ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca gccatcgggt
    74821 tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg
    74881 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
    74941 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
    75001 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
    75061 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg ttttctgccg
    75121 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
    75181 ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat ttttcatgca
    75241 actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc cagagaggta
    75301 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
    75361 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
    75421 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
    75481 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
    75541 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
    75601 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
    75661 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
    75721 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
    75781 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
    75841 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
    75901 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
    75961 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
    76021 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
    76081 aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag cttgttggcg
    76141 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
    76201 gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg
    76261 aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg atttttaatc
    76321 ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat aaagccactg
    76381 gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc cgggctctgg
    76441 cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac cgggtcgggc
    76501 gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct
    76561 tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag
    76621 ataaaacaac tagggcagat aaaatgccaa tgctaattaa tcacgctttt ccagcagagt
    76681 ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag
    76741 ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac
    76801 agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc
    76861 gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa
    76921 gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga
    76981 atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc
    77041 actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct gggcaattac
    77101 gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc
    77161 cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca gaacggtcaa
    77221 accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag
    77281 tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa
    77341 ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaa
//
LOCUS       DAECOD010000003        71684 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.003, whole genome shotgun sequence.
ACCESSION   DAECOD010000003 DAECOD010000000
VERSION     DAECOD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.003"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KKO15_000171"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KKO15_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD6359111.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KKO15_000172"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KKO15_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBD6359112.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KKO15_000173"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KKO15_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBD6359113.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KKO15_000174"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KKO15_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBD6359114.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KKO15_000175"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KKO15_000175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBD6359115.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KKO15_000176"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KKO15_000176"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBD6359116.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KKO15_000177"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KKO15_000177"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBD6359117.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="KKO15_000178"
     CDS             complement(4598..5320)
                     /locus_tag="KKO15_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6359118.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMAGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KKO15_000179"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KKO15_000179"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6359119.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="KKO15_000180"
     CDS             6520..6738
                     /locus_tag="KKO15_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBD6359120.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KKO15_000181"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KKO15_000181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD6359121.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="KKO15_000182"
     CDS             complement(7472..7672)
                     /locus_tag="KKO15_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBD6359122.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KKO15_000183"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KKO15_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KKO15_000184"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KKO15_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBD6359123.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="KKO15_000185"
     CDS             10101..12014
                     /locus_tag="KKO15_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6359124.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="KKO15_000186"
     CDS             12014..13036
                     /locus_tag="KKO15_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6359125.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLANEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="KKO15_000187"
     CDS             13030..13248
                     /locus_tag="KKO15_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBD6359126.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="KKO15_000188"
     CDS             13302..14171
                     /locus_tag="KKO15_000188"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBD6359127.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSRETVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="KKO15_000189"
     CDS             complement(14226..14630)
                     /locus_tag="KKO15_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBD6359128.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="KKO15_000190"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="KKO15_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBD6359129.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="KKO15_000191"
     CDS             15615..17705
                     /locus_tag="KKO15_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD6359130.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLRLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KKO15_000192"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KKO15_000192"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBD6359131.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KKO15_000193"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KKO15_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBD6359132.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="KKO15_000194"
     CDS             complement(19673..20275)
                     /locus_tag="KKO15_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBD6359133.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="KKO15_000195"
     CDS             complement(20265..20432)
                     /locus_tag="KKO15_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBD6359134.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KKO15_000196"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KKO15_000196"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBD6359135.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="KKO15_000197"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="KKO15_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBD6359136.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="KKO15_000198"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="KKO15_000198"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBD6359137.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="KKO15_000199"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="KKO15_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBD6359138.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="KKO15_000200"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="KKO15_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBD6359139.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="KKO15_000201"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="KKO15_000201"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD6359140.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="KKO15_000202"
     CDS             28269..28436
                     /locus_tag="KKO15_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBD6359141.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="KKO15_000203"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="KKO15_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="KKO15_000204"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="KKO15_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBD6359142.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="KKO15_000205"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="KKO15_000205"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBD6359143.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="KKO15_000206"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="KKO15_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBD6359144.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQLGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="KKO15_000207"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="KKO15_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBD6359145.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="KKO15_000208"
     CDS             complement(33764..34849)
                     /locus_tag="KKO15_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD6359146.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="KKO15_000209"
     CDS             complement(34861..36165)
                     /locus_tag="KKO15_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBD6359147.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="KKO15_000210"
     CDS             complement(36177..36530)
                     /locus_tag="KKO15_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBD6359148.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="KKO15_000211"
     CDS             complement(36541..37419)
                     /locus_tag="KKO15_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBD6359149.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="KKO15_000212"
     CDS             complement(37416..38642)
                     /locus_tag="KKO15_000212"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD6359150.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQLSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="KKO15_000213"
     CDS             complement(38643..39806)
                     /locus_tag="KKO15_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBD6359151.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="KKO15_000214"
     CDS             complement(39890..40252)
                     /locus_tag="KKO15_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBD6359152.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="KKO15_000215"
     CDS             complement(40269..41174)
                     /locus_tag="KKO15_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359153.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KKO15_000216"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KKO15_000216"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBD6359154.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KKO15_000217"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KKO15_000217"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBD6359155.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KKO15_000218"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KKO15_000218"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBD6359156.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KKO15_000219"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KKO15_000219"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD6359157.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46116)
                     /gene="damX"
                     /locus_tag="KKO15_000220"
     CDS             complement(44830..46116)
                     /gene="damX"
                     /locus_tag="KKO15_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBD6359158.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="KKO15_000221"
     CDS             complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="KKO15_000221"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBD6359159.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="KKO15_000222"
     CDS             complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="KKO15_000222"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBD6359160.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="KKO15_000223"
     CDS             complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="KKO15_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBD6359161.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="KKO15_000224"
     CDS             complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="KKO15_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBD6359162.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="KKO15_000225"
     CDS             complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="KKO15_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBD6359163.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="KKO15_000226"
     CDS             complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="KKO15_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBD6359164.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="KKO15_000227"
     CDS             complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="KKO15_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBD6359165.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51681..54233
                     /gene="mrcA"
                     /locus_tag="KKO15_000228"
     CDS             51681..54233
                     /gene="mrcA"
                     /locus_tag="KKO15_000228"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBD6359166.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGDAQELF"
     gene            complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="KKO15_000229"
     CDS             complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="KKO15_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBD6359167.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55278..57413
                     /gene="igaA"
                     /locus_tag="KKO15_000230"
     CDS             55278..57413
                     /gene="igaA"
                     /locus_tag="KKO15_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBD6359168.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57478..58146
                     /gene="yrfG"
                     /locus_tag="KKO15_000231"
     CDS             57478..58146
                     /gene="yrfG"
                     /locus_tag="KKO15_000231"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBD6359169.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58157..58558
                     /gene="hslR"
                     /locus_tag="KKO15_000232"
     CDS             58157..58558
                     /gene="hslR"
                     /locus_tag="KKO15_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBD6359170.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58583..59452
                     /gene="hslO"
                     /locus_tag="KKO15_000233"
     CDS             58583..59452
                     /gene="hslO"
                     /locus_tag="KKO15_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBD6359171.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59596..61320)
                     /locus_tag="KKO15_000234"
     CDS             complement(59596..61320)
                     /locus_tag="KKO15_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBD6359172.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61699..62007
                     /locus_tag="KKO15_000235"
                     /pseudo
     CDS             61699..62007
                     /locus_tag="KKO15_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
     gene            complement(62185..63537)
                     /gene="envZ"
                     /locus_tag="KKO15_000236"
     CDS             complement(62185..63537)
                     /gene="envZ"
                     /locus_tag="KKO15_000236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBD6359173.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(63534..64253)
                     /gene="ompR"
                     /locus_tag="KKO15_000237"
     CDS             complement(63534..64253)
                     /gene="ompR"
                     /locus_tag="KKO15_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBD6359174.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            64481..64957
                     /gene="greB"
                     /locus_tag="KKO15_000238"
     CDS             64481..64957
                     /gene="greB"
                     /locus_tag="KKO15_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBD6359175.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            65054..67375
                     /locus_tag="KKO15_000239"
     CDS             65054..67375
                     /locus_tag="KKO15_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBD6359176.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            67813..68040
                     /gene="feoA"
                     /locus_tag="KKO15_000240"
     CDS             67813..68040
                     /gene="feoA"
                     /locus_tag="KKO15_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBD6359177.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            68057..70378
                     /gene="feoB"
                     /locus_tag="KKO15_000241"
     CDS             68057..70378
                     /gene="feoB"
                     /locus_tag="KKO15_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBD6359178.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            70378..70614
                     /gene="feoC"
                     /locus_tag="KKO15_000242"
     CDS             70378..70614
                     /gene="feoC"
                     /locus_tag="KKO15_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBD6359179.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            70817..>71684
                     /gene="rpnA"
                     /locus_tag="KKO15_000243"
     CDS             70817..>71684
                     /gene="rpnA"
                     /locus_tag="KKO15_000243"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBD6359180.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17288 a        19408 c        18122 g        16866 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca aaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccaaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgccatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaatga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aaacagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactac gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtaat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacac aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc agattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc agagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagctg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacagctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt
    45301 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt
    45361 ggacggacgt tcagcggttt gcgttttcgc cgtgtcacgc gatgcattgc cattgcgaac
    45421 aggcgcaacc gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg
    45481 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    45541 ttgaccatcc gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag
    45601 agaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    45661 cgcctgatcg gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc
    45721 gctggaagtg gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    45781 aaccagaatg ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    45841 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    45901 gtcaatttct tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg
    45961 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    46021 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    46081 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    46141 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    46201 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    46261 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    46321 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    46381 cattgaccgg taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac
    46441 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    46501 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    46561 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct
    46621 tcagttcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    46681 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    46741 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    46801 ccaccaccac tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    46861 tgaccgcagt tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt
    46921 gaatgaaacg aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta
    46981 cgccgccgcc aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg
    47041 taaagacggt atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc
    47101 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    47161 ccagggtttc gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    47221 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    47281 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    47341 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    47401 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    47461 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    47521 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    47581 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    47641 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    47701 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    47761 ctcttgatcg gaatcgtaaa attccatatt gagttgttga gctaactggc gcccaatagt
    47821 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    47881 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    47941 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    48001 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    48061 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    48121 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    48181 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    48241 ttcagcataa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    48301 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    48361 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    48421 taaaaatgcc gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct
    48481 cctgcttatc aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt
    48541 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct
    48601 ccatccccag gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa
    48661 cctgatatgg aatttcgctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca
    48721 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    48781 ccagcaagcg tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta
    48841 cggagaggtc gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg
    48901 ccagcgtcca tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg
    48961 ccgacagctc aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc
    49021 ttaacgccgt tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac
    49081 tccctttggc actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt
    49141 gcagggttat actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct
    49201 cctggcgggc gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt
    49261 cctgccgcgt tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    49321 catctgttag atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact
    49381 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    49441 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    49501 tccattgctt catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt
    49561 gtcccggtac ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc
    49621 agcacatcgt tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg
    49681 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    49741 catagatctt ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata
    49801 cctgccaaca accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag
    49861 atcatcacct tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg
    49921 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc
    49981 ggatggatgc caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    50041 cagcgttttt tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg
    50101 ctggtgatga cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga
    50161 tagaaaaatg agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg
    50221 gggtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    50281 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat
    50341 gaaaagacgc atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg
    50401 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct
    50461 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    50521 ggcgctgccg ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg
    50581 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg
    50641 cttcggcgct gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    50701 caacgaacat cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca
    50761 aaaaattaat tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc
    50821 aaagtctcca ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc
    50881 aaatccgcct tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag
    50941 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    51001 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    51061 gaatcgctgt aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc
    51121 tgccagcgta agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg
    51181 gctgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg
    51241 ggccatcgtc cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg
    51301 cggaaatgac cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg
    51361 cggcagttcg cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg
    51421 cccatctttg ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc
    51481 ttttgcgccc cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat
    51541 ttgccagatc ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat
    51601 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    51661 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    51721 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    51781 cggatgtggt gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    51841 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    51901 tcccaccgga gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc
    51961 acggagttga cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg
    52021 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag
    52081 aacgcacgat gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    52141 tgacgaaaga cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct
    52201 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga
    52261 acgaaatggc ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact
    52321 caatggatcg tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt
    52381 atatcaccca gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg
    52441 cgccggagat tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata
    52501 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    52561 aagtgcagca ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc
    52621 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca
    52681 acaagattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    52741 tcaccagcgc caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat
    52801 tgagtatgga aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga
    52861 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg
    52921 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    52981 cgcaaaatgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    53041 accgcgccac ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    53101 cggcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct
    53161 gggatgcagg tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc
    53221 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc
    53281 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa
    53341 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    53401 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    53461 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    53521 aatgcgatat tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    53581 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    53641 tggagcaggc aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg
    53701 tcatcaacac tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg
    53761 aaccaggctg gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg
    53821 gcgggaaaac cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    53881 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    53941 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    54001 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga
    54061 cgccgcctcc gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg
    54121 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc
    54181 acgaggtggg aacgaccatt atcgataatg gtgatgcaca ggaattgttc tgattaaaaa
    54241 ggcgcttcgg cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt
    54301 gaacgcgtta tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag
    54361 cctgataagc gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca
    54421 accattcgcg cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt
    54481 ccagcaaatc catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat
    54541 cgccttccag cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa
    54601 agtaagacgg tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa
    54661 cctcttcttt tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc
    54721 ctttcgaaaa acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca
    54781 ggtgatcgtc cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt
    54841 cataaacacg ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc
    54901 gggaacgggc tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg
    54961 tgggatctat gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg
    55021 tgaattgcat gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta
    55081 aaagtcaata ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg
    55141 ctatgatcag cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc
    55201 gatagccaac cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta
    55261 agtaagatgg ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct
    55321 cactacttgc gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca
    55381 acgccttcgc ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa
    55441 attatcttga gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg
    55501 caccgatctc tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta
    55561 tcacacgtta cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg
    55621 tagaagtcca cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac
    55681 tgattcatac cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt
    55741 acatgcagga aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc
    55801 gcggggaaga gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat
    55861 atgcgctgag tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga
    55921 tgttcttttt ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat
    55981 tactgctgct gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc
    56041 gagaaattca ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc
    56101 aggaacagat caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc
    56161 agccatacat tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc
    56221 acgtagtgcg tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac
    56281 agcactggct gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt
    56341 tattctggat cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc
    56401 agaccattga agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata
    56461 cattgcgtat tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga
    56521 aaaccaattc acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct
    56581 cattaccatt accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta
    56641 atcgccagct gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag
    56701 cgattcaaaa atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag
    56761 cggatttatg ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg
    56821 gcaacagtaa agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg
    56881 tgaatgtgtt attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca
    56941 ccgcgccgtt tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc
    57001 ccggcggatt cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt
    57061 cggcatcact ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac
    57121 aaatgctgat gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg
    57181 ccaatggtac gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct
    57241 atctcagcac cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac
    57301 aggcctggcg tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg
    57361 aaagctgcct gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt
    57421 gcgcggcagg ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg
    57481 catatcaaca ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg
    57541 ctcgacctcg ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg
    57601 aaaaacgggg ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta
    57661 cagcatacgc taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc
    57721 tgtgcgatga ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt
    57781 cttgaggcac tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac
    57841 aacctggcgg taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc
    57901 acccacacat ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct
    57961 acgggtctga aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct
    58021 gccgcgcaat ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc
    58081 gagaaacagt atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta
    58141 atgtgaagga gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct
    58201 atgggctgcc cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa
    58261 ggtgcattac aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct
    58321 cactctgcgc cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg
    58381 tcgccccgcc agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg
    58441 cgaaaaaatg gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg
    58501 cccggacaaa aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact
    58561 gtcacctgca agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga
    58621 aaattttgcc gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga
    58681 gaaccacgat tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag
    58741 cctgttaacc gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg
    58801 tccgatgaat ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg
    58861 cgtgcagggc gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt
    58921 ggtgatcacc attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg
    58981 tgataccctg gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg
    59041 cctgtttatt cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca
    59101 ggtaatgcct gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga
    59161 aaccatcaaa accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta
    59221 tcacgaagaa gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc
    59281 gcgtgaacgt tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct
    59341 ggcggaagat ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa
    59401 tgcgatggat attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta
    59461 atgagtatgt ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct
    59521 gcgcttacca gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat
    59581 ccgataaaag cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag
    59641 atcgcgccac gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt
    59701 ttctttcgtt atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc
    59761 tgagtcaaag ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc
    59821 ctgaccatca ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
    59881 catcacgtta tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga
    59941 accaggggca attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg
    60001 ttgctctcca tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc
    60061 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    60121 gtagatagtt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    60181 cacgctaata cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac
    60241 caataacgaa accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg
    60301 ccagacgata ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc
    60361 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt
    60421 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    60481 ccccgtccag ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    60541 caaaaaggcc agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag
    60601 gcccgtaaac ggcagggtga tgataaacat cagggttagc aatgatacta acggcagcaa
    60661 ccccgtggcg attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    60721 cgcgaggatc accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt
    60781 tgcaaaaaag agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag
    60841 taaaagcacc agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt
    60901 atgccatact gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct
    60961 ttgttgtatc cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca
    61021 aatcgccttt tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct
    61081 catccctgtc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    61141 agagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    61201 aatccaactg tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg
    61261 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    61321 gttggttatc cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct
    61381 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt
    61441 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    61501 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    61561 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    61621 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    61681 tggctaagga gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct
    61741 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    61801 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    61861 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    61921 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    61981 aaacctctct ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc
    62041 gacaacttcg ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg
    62101 aaactgtaat gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg
    62161 gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac
    62221 cggcactggc agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag
    62281 catcccgtta tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct
    62341 aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg
    62401 cgcaattccc ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct
    62461 gctgactttg atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc
    62521 gcgtttgatc gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc
    62581 aatttcccgc tcatagccac tttcggcagc aatcacctca ccgagcactg cattaagatc
    62641 cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat
    62701 ggcgttgcac tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct
    62761 catcatctca gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc
    62821 cgccatcagc agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa
    62881 ggcacgggta acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat
    62941 ccctttacca acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg
    63001 aataaacagc cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag
    63061 cggagagaaa tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga
    63121 cagccaggtt ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt
    63181 cgggccgccc agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg
    63241 cagacctgcc tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat
    63301 ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc
    63361 ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa
    63421 aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac
    63481 gatgagcaat aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct
    63541 ttagagccgt ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga
    63601 tgcgctggat cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt
    63661 tccattgcgg aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc
    63721 tcacgcggat ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc
    63781 ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca
    63841 attaccgcct cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc
    63901 gcacgaatac gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg
    63961 ccaatctcca ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc
    64021 ggcatcgggt tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt
    64081 aacattaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt
    64141 tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc
    64201 gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt
    64261 actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca
    64321 ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct
    64381 taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc
    64441 caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac
    64501 ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc
    64561 ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga
    64621 ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa
    64681 atgcctggaa aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt
    64741 tggcgcgtgg gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg
    64801 ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc
    64861 attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgaa
    64921 ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg
    64981 atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag
    65041 atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc
    65101 gcgcccggaa caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt
    65161 tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct
    65221 ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc
    65281 catttccgag caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag
    65341 caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca
    65401 aatcgccatt gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca
    65461 cacgccagaa gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa
    65521 agccgcgctg gatggcgcgc gctatatcct gatggaacgg tttgccgaag atgccgcgct
    65581 gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta cggtggtgag
    65641 cggtaaagaa gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgttgtc
    65701 cacggtgcct tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca
    65761 gctttcgctg aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca
    65821 aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa
    65881 aagcgtagtg agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat
    65941 gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca
    66001 cgatctgctg atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct
    66061 gcgtaccggg gtaaaagtgg cggtggtcga tgccactggc aaactggtgg cgaccgatac
    66121 catttacccg cacaccggac aagccgcaaa agcagcgatg accgttgctg ccttgtgtga
    66181 aaaacataac gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaactgaacg
    66241 tttctatctc gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag
    66301 cgaagctggc gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct
    66361 cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga
    66421 gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca
    66481 gacgcaactg gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt
    66541 cgatctcaac accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat
    66601 ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact
    66661 gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat
    66721 taaccacggt gataacccgc tggacgcctc taccgttcat ccggaagcct atccggtggt
    66781 ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga
    66841 actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac
    66901 tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc
    66961 tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg
    67021 cgcagtgacc aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg
    67081 cctggttcac atctcttcat tatcgaacaa gtttgtggaa gatccgcata ccgtggtgaa
    67141 agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc
    67201 cctgactatg cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa
    67261 tgaacgcccg caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc
    67321 cggtaatagc gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc
    67381 cggactggcc cctaaacctg cgcgataagg cgttcacgcc gcatctggca acgaacgctt
    67441 tgcctgacat aaaagtgccg gagaatatct ccggcacttt tattccacag ccaaactcac
    67501 aatatactcc gttaatattt atcacttcat taacaactga aaccttaatt aaacattagc
    67561 cagtcctggt aactcactat tcgaattata ttttcgctgc gatataacct tgagccacat
    67621 caacattgag tcagattatt attcaaacca acattcgcac acattttaag tattgctgat
    67681 agaaaccatt ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat
    67741 catctggtct catgtcgcgg tcaaacgccc catgaggtag ttatccagtt aatgagaaac
    67801 aagtaggcac ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt
    67861 gaaatcagcc cggcatatcg ccaaaaactg ctttctctag gcatgttacc tggctcctct
    67921 tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg
    67981 agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa
    68041 tacggtgata acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg
    68101 caagacaacc ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg
    68161 cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt
    68221 ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca
    68281 aatcgcctgt cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc
    68341 gtctaacctt gagcgtaacc tgtacctgac gctgcaactg ctggaactcg gcattccctg
    68401 catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga
    68461 tgctctatcg gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg
    68521 tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt
    68581 gcattacgca cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga
    68641 catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag
    68701 ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa
    68761 tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat
    68821 ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga
    68881 taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat
    68941 gttcctgctg gctatcaaca tcggcggggc gttacagccg ctgtttaacg tcggctccgt
    69001 ggcgctattt gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct
    69061 gactatcttc ctcgcccagg gcctgggtgg cggtattaac accgtgctgc cactggtgcc
    69121 gcagatcggc atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg
    69181 tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc ttgccgggga aatcctttgt
    69241 gccgctgatc gtcggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga
    69301 tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg
    69361 tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg ccctggcggt
    69421 tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg actggcctga tgctcaagta
    69481 caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc
    69541 acacgttaaa agcctgatta tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc
    69601 tggtaaagtg attatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgttgag
    69661 cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgataac
    69721 ccctgtcttc aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt
    69781 tacgggcgca atggcaaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga
    69841 aaatattcag gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag
    69901 tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa
    69961 ccccattgaa gctagcaaag gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga
    70021 tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt
    70081 accatgtatc tcggtgatgg gagccatcgc ccgtgaatca agccgtggct ggatgggctt
    70141 ctccatcctg tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc
    70201 cagctacagt cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa
    70261 catcgtagct atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc
    70321 aacccgcaag tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg
    70381 gcttcactta ttcaggtacg tgatttgctg gcgttacggg gccgtatgga agcgacccag
    70441 ataagccaga cattgaacac tccgcagcca atgattaacg ccatgctgca acagctggaa
    70501 agcatgggca aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt
    70561 aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac
    70621 tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc
    70681 tttccataaa aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg
    70741 gatgaaaact cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa
    70801 caggtgaagg aacgccatga gtaaaaagca gagttccacc ccacatgatg cgctgttcaa
    70861 actcttttta cgccaaccgg agacggcacg cgattttctt gcgtttcatt taccggcacc
    70921 cattcacgcg ctctgtgata tgaaaaccct caagctggag tcgaggagct ttattgatga
    70981 cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg
    71041 atacatctat tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat
    71101 gatgcgttac gctattgccg caatgcaaaa tcaccttgat gctggataca aaacgttgcc
    71161 gatggtggtg ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg
    71221 ttggctggat tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc
    71281 gctgattgat gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct
    71341 gctggagtta attcaaaaac atattcgtca acgcgatctg atggggctgg tagagcaaat
    71401 ggcctgctta ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta
    71461 catactgcaa actggcgacg ctgtacgttt taacgatttt atcgacggcg ttgccgaacg
    71521 ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa agattgcggc aggaggggga
    71581 acaatccaaa gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga
    71641 catcatgcga tttaccgggc tgtcagaaga agagttggct gcgg
//
LOCUS       DAECOD010000004        70699 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.004, whole genome shotgun sequence.
ACCESSION   DAECOD010000004 DAECOD010000000
VERSION     DAECOD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.004"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..795
                     /locus_tag="KKO15_000244"
     CDS             <1..795
                     /locus_tag="KKO15_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6359181.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="KKO15_000245"
     CDS             806..1897
                     /locus_tag="KKO15_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6359182.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KKO15_000246"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KKO15_000246"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBD6359183.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="KKO15_000247"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="KKO15_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBD6359184.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="KKO15_000248"
     CDS             4859..5254
                     /locus_tag="KKO15_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBD6359185.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="KKO15_000249"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="KKO15_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBD6359186.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="KKO15_000250"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="KKO15_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBD6359187.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="KKO15_000251"
     CDS             complement(6563..7603)
                     /locus_tag="KKO15_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD6359188.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="KKO15_000252"
     CDS             complement(7676..8311)
                     /locus_tag="KKO15_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD6359189.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="KKO15_000253"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="KKO15_000253"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBD6359190.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="KKO15_000254"
     CDS             complement(8937..9380)
                     /locus_tag="KKO15_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBD6359191.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="KKO15_000255"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="KKO15_000255"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBD6359192.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="KKO15_000256"
     CDS             complement(9720..10223)
                     /locus_tag="KKO15_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6359193.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="KKO15_000257"
     CDS             complement(10217..10741)
                     /locus_tag="KKO15_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBD6359194.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="KKO15_000258"
     CDS             10950..11945
                     /locus_tag="KKO15_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD6359195.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="KKO15_000259"
     CDS             11954..12832
                     /locus_tag="KKO15_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD6359196.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="KKO15_000260"
     CDS             12913..13920
                     /locus_tag="KKO15_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD6359197.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KKO15_000261"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KKO15_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBD6359198.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KKO15_000262"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KKO15_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBD6359199.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KKO15_000263"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KKO15_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBD6359200.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KKO15_000264"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KKO15_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBD6359201.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KKO15_000265"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KKO15_000265"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBD6359202.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KKO15_000266"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KKO15_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBD6359203.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KKO15_000267"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KKO15_000267"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBD6359204.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KKO15_000268"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KKO15_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBD6359205.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KKO15_000269"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KKO15_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBD6359206.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KKO15_000270"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KKO15_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBD6359207.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KKO15_000271"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KKO15_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBD6359208.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="KKO15_000272"
     tRNA            complement(27654..27730)
                     /locus_tag="KKO15_000272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="KKO15_000273"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="KKO15_000273"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBD6359209.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="KKO15_000274"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="KKO15_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="KKO15_000275"
     tRNA            complement(31514..31600)
                     /locus_tag="KKO15_000275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="KKO15_000276"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="KKO15_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBD6359210.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="KKO15_000277"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="KKO15_000277"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBD6359211.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="KKO15_000278"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="KKO15_000278"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD6359212.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="KKO15_000279"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="KKO15_000279"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBD6359213.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="KKO15_000280"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="KKO15_000280"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBD6359214.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="KKO15_000281"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="KKO15_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBD6359215.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="KKO15_000282"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="KKO15_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBD6359216.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="KKO15_000283"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="KKO15_000283"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD6359217.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41184)
                     /gene="cgtA"
                     /locus_tag="KKO15_000284"
     CDS             complement(40024..41184)
                     /gene="cgtA"
                     /locus_tag="KKO15_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBD6359218.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDEDDEEGVEFIYKR"
     gene            complement(41200..42165)
                     /locus_tag="KKO15_000285"
     CDS             complement(41200..42165)
                     /locus_tag="KKO15_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD6359219.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42292..42549)
                     /gene="rpmA"
                     /locus_tag="KKO15_000286"
     CDS             complement(42292..42549)
                     /gene="rpmA"
                     /locus_tag="KKO15_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBD6359220.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42570..42881)
                     /gene="rplU"
                     /locus_tag="KKO15_000287"
     CDS             complement(42570..42881)
                     /gene="rplU"
                     /locus_tag="KKO15_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBD6359221.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43139..44110
                     /gene="ispB"
                     /locus_tag="KKO15_000288"
     CDS             43139..44110
                     /gene="ispB"
                     /locus_tag="KKO15_000288"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBD6359222.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44337..44615
                     /gene="sfsB"
                     /locus_tag="KKO15_000289"
     CDS             44337..44615
                     /gene="sfsB"
                     /locus_tag="KKO15_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBD6359223.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44663..45922)
                     /gene="murA"
                     /locus_tag="KKO15_000290"
     CDS             complement(44663..45922)
                     /gene="murA"
                     /locus_tag="KKO15_000290"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBD6359224.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45977..46231)
                     /gene="ibaG"
                     /locus_tag="KKO15_000291"
     CDS             complement(45977..46231)
                     /gene="ibaG"
                     /locus_tag="KKO15_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBD6359225.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46391..46684)
                     /gene="mlaB"
                     /locus_tag="KKO15_000292"
     CDS             complement(46391..46684)
                     /gene="mlaB"
                     /locus_tag="KKO15_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBD6359226.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46684..47319)
                     /gene="mlaC"
                     /locus_tag="KKO15_000293"
     CDS             complement(46684..47319)
                     /gene="mlaC"
                     /locus_tag="KKO15_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBD6359227.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47338..47889)
                     /gene="mlaD"
                     /locus_tag="KKO15_000294"
     CDS             complement(47338..47889)
                     /gene="mlaD"
                     /locus_tag="KKO15_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBD6359228.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47894..48676)
                     /gene="mlaE"
                     /locus_tag="KKO15_000295"
     CDS             complement(47894..48676)
                     /gene="mlaE"
                     /locus_tag="KKO15_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBD6359229.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48684..49493)
                     /gene="mlaF"
                     /locus_tag="KKO15_000296"
     CDS             complement(48684..49493)
                     /gene="mlaF"
                     /locus_tag="KKO15_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBD6359230.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49703..50668
                     /locus_tag="KKO15_000297"
     CDS             49703..50668
                     /locus_tag="KKO15_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBD6359231.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50682..51668
                     /gene="kdsD"
                     /locus_tag="KKO15_000298"
     CDS             50682..51668
                     /gene="kdsD"
                     /locus_tag="KKO15_000298"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBD6359232.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51689..52255
                     /gene="kdsC"
                     /locus_tag="KKO15_000299"
     CDS             51689..52255
                     /gene="kdsC"
                     /locus_tag="KKO15_000299"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBD6359233.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52252..52827
                     /gene="lptC"
                     /locus_tag="KKO15_000300"
     CDS             52252..52827
                     /gene="lptC"
                     /locus_tag="KKO15_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBD6359234.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52796..53353
                     /gene="lptA"
                     /locus_tag="KKO15_000301"
     CDS             52796..53353
                     /gene="lptA"
                     /locus_tag="KKO15_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBD6359235.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53360..54085
                     /gene="lptB"
                     /locus_tag="KKO15_000302"
     CDS             53360..54085
                     /gene="lptB"
                     /locus_tag="KKO15_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBD6359236.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54133..55566
                     /gene="rpoN"
                     /locus_tag="KKO15_000303"
     CDS             54133..55566
                     /gene="rpoN"
                     /locus_tag="KKO15_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBD6359237.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55589..55876
                     /gene="hpf"
                     /locus_tag="KKO15_000304"
     CDS             55589..55876
                     /gene="hpf"
                     /locus_tag="KKO15_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBD6359238.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55994..56485
                     /gene="ptsN"
                     /locus_tag="KKO15_000305"
     CDS             55994..56485
                     /gene="ptsN"
                     /locus_tag="KKO15_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBD6359239.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56531..57385
                     /gene="rapZ"
                     /locus_tag="KKO15_000306"
     CDS             56531..57385
                     /gene="rapZ"
                     /locus_tag="KKO15_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBD6359240.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57382..57654
                     /gene="npr"
                     /locus_tag="KKO15_000307"
     CDS             57382..57654
                     /gene="npr"
                     /locus_tag="KKO15_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBD6359241.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57867..58499
                     /gene="yrbL"
                     /locus_tag="KKO15_000308"
     CDS             57867..58499
                     /gene="yrbL"
                     /locus_tag="KKO15_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBD6359242.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58496..59223)
                     /gene="mtgA"
                     /locus_tag="KKO15_000309"
                     /pseudo
     CDS             complement(58496..59223)
                     /gene="mtgA"
                     /locus_tag="KKO15_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59220..59873)
                     /gene="elbB"
                     /locus_tag="KKO15_000310"
     CDS             complement(59220..59873)
                     /gene="elbB"
                     /locus_tag="KKO15_000310"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBD6359243.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60103..62439)
                     /gene="arcB"
                     /locus_tag="KKO15_000311"
     CDS             complement(60103..62439)
                     /gene="arcB"
                     /locus_tag="KKO15_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBD6359244.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62535..63464)
                     /locus_tag="KKO15_000312"
     CDS             complement(62535..63464)
                     /locus_tag="KKO15_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBD6359245.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDVVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64139..68599
                     /gene="gltB"
                     /locus_tag="KKO15_000313"
     CDS             64139..68599
                     /gene="gltB"
                     /locus_tag="KKO15_000313"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBD6359246.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68612..70030
                     /gene="gltD"
                     /locus_tag="KKO15_000314"
     CDS             68612..70030
                     /gene="gltD"
                     /locus_tag="KKO15_000314"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBD6359247.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70214..70693
                     /locus_tag="KKO15_000315"
     CDS             70214..70693
                     /locus_tag="KKO15_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBD6359248.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19171 c        17555 g        16659 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtagtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 acagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcctgca tggaacccta
    22801 aaaaccttaa gcgatggtac gctggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcttcatc
    40081 atcctcttca gcaatctctt caagctgctg acggtgataa tcatcccaca tgaattcgac
    40141 tttctctggc tgtttcgctt cttcagcctg cacgaccggg ttttcaatga taaaggtcat
    40201 cacatcccag cagagatctt tcacgcccag tccgctcgcc gcagagatca gataatattt
    40261 atcttcccag cccagcgctt cagcgatcgc tttcgctttc tcttcggctt ctgccttatc
    40321 cagcagatcg atcttgttga agactaacca acgcggtttc gccgccagat cctggctgta
    40381 tttttccagc tcgctgataa taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg
    40441 atcgatatcg atgaggtgca acaggacgcg gcaacgttcc aggtgcttca ggaagcgaat
    40501 gcccagacct gcgccttccg cagcgccttc aatcagtcct ggaatatcgg caacaacgaa
    40561 gctcttttcg ttgtccattc gtaccacacc cagacttggc accagagtgg taaacggata
    40621 atccgccact ttcggtttag ccgccgatac cgcacgaata aaggtcgatt tacccgcgtt
    40681 tggcatcccc aacataccga cgtcagccag cagcatcagc tccagcagca gctcacgctt
    40741 atcgcccggc gtaccgttag ttttctgccg tggtgtacgg ttaactgacg atttgaaacg
    40801 ggtattgccc agaccgtgcc agccgccctt agcaaccagc agacgctgac cgtgtttagt
    40861 catatcaccc atggtttcac cagtaccctg gtcgattaca cgcgtaccta ccggcacttt
    40921 aatcgtcacg tctttaccgc gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg
    40981 ctctgcacgg aaagattttt caaaacgata atcgataagc gtgttcaggt tctcgtcggc
    41041 ttccatccat acgtcaccac catcaccgcc gtcgccgcca tccgggccgc ctttcggaat
    41101 atacttttcg cggcggaagc tcacgcaacc attaccgcca tcacctgcaa cgaccagaat
    41161 cgatgcttca tcaacaaact tcattttatt ctccgtaaat cattcgcctg cgcggggttg
    41221 cgataccacc gttgtatgct tacgtaatcc gccccaaata cgatgaccaa tggcggaata
    41281 catcgcgccc gcaaccacga caaacgcacc gagataacct aaaaggttta acatcggtct
    41341 ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa aaaaacagcg taaacagtgg
    41401 ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag cgagccatcg cttccgccaa
    41461 ggcgccatat cctaccaagg tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg
    41521 gctaagctgc gctatcactc caggctttgc cagagggaag agcgcgattg tacataaagt
    41581 gtacagtaaa aacaggatct gcggtgaggc cagccgacgc aataaaacct tttgcgccac
    41641 gccataactc acccaaaccg tcgccgcacc gaccccaaag ataactcccc aggtgtaatc
    41701 ggtgagcttt gtaaatatct cgaccagact ggtgttaaaa aacatcacca gaccgctcag
    41761 gagcatcaat gcccctacaa cctgagtgct gcgcattttc tctttcagga taaatacgct
    41821 ggcaaccatc atgccaactg gcgagagttg cccaatcacc tgcgaagcgg tcggactcag
    41881 gtattgcaag gatgagctga acaggatgaa gttcccaaac agcccggcgg tcgccactgc
    41941 caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac
    42001 cgcaagaatg gcaccaaggc caatactcgc catcaagaaa cggtaaaaca cgattgtcgg
    42061 aggttccatc acctccagca cctgcttcat tgcgattggc aacgcccccc agcaaattgc
    42121 tgtggtaagc gccaaaagaa tgccaatgcc tgcctgctgc ttcataccgt attccctgca
    42181 aagcgcattt gcggattaac gtcgcaccat gcgcattttt accggttatc gaatgtaaaa
    42241 agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg acgcgaaaaa cttattcagc
    42301 ttcgatgctg ataaatttac ggtttttcgg gcctttaact tcgaatttca ctttaccgtc
    42361 tgctttagca aacagagtgt ggtcacgacc gcaacctacg ttagcgccag cgtggaattt
    42421 ggtaccacgt tgacgaacga tgatgctacc cgccagaacg gattcgccac cgaaacgctt
    42481 aacgcccaag cgtttagctt ctgaatcgcg accgttacgt gtggagccgc cagccttttt
    42541 atgtgccatt tgaaatctct cctcaggtct taggcgctga tgccagtaat tttcacatca
    42601 gtgaaccact gacgatggcc ctgctgctta cgatagtgtt tacgacgacg aaacttaacg
    42661 attttaactt tctcgccacg accgtgagca acaacttcag ctttgattac gccgccatca
    42721 acgaaaggaa cgccgatttt gacttcttca ccgtttgcga tcatcagcac ttcagcgaac
    42781 tcaacagttt cgccagttgc gatgtccagc ttttccaggc gaacggtctg accttcgctt
    42841 actcggtgtt gtttaccacc actttggaaa accgcgtaca tataaaactc cgcttccgcg
    42901 cacacctttt caatgattca gagtgcgcta taaatattca caatagggcg cgaatattac
    42961 gcaaaacgca cgcctttgac aagtgctaca gtcaatacac gaagaaaaaa acacaacttg
    43021 tacggtaacg tttatctgtg ccattttttc agtacaatta ccctatattc ctaaccataa
    43081 accctaagtt gcctttgttc acagtaaggt aatcggggcg aaaagcccgg cttttgcgat
    43141 gaatttagaa aaaatcaatg agttaaccgc gcaagatatg gcgggtgtta atgcggcaat
    43201 ccttgagcag cttaattccg acgtccaact gatcaatcag ttaggctatt acatcgtcag
    43261 cggcggcggt aaacgtattc gtccgatgat tgctgtactg gctgcacgag ctgttggcta
    43321 tgagggaaat gcgcatgtca ccattgctgc cctgatcgag tttatccaca cggcgactct
    43381 gctacacgac gacgttgtgg atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc
    43441 cgcatttggc aatgccgcca gcgtgctggt aggcgatttt atttataccc gcgcgttcca
    43501 gatgatgacc agcctcggtt cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt
    43561 catcgcagaa ggtgaagttc tgcaactgat gaacgttaac gatccggaca tcactgaaga
    43621 aaactacatg cgcgttatct atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg
    43681 ttccgggatt ctggctggct gtacgccgga ggaggagaaa ggcctgcagg attatgggcg
    43741 ctatctcggc actgctttcc agttgatcga cgatttactc gattacaatg ccgatggcga
    43801 acagttaggt aaaaacgtcg gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct
    43861 gcatgcgatg catcatggca caccagaaca ggcacagatg atccgtaccg ccatcgaaca
    43921 aggtaacggt cgccatcttc tggaaccggt tctggaagca atgaacgctt gtggatctct
    43981 tgaatggacg cgtcagcgcg ccgaggaaga agcagacaaa gccatcgcag cgttacaggt
    44041 gctcccggac accccttggc gagaagcact catcggcctc gcgcacatcg ctgttcaacg
    44101 cgatcgttaa tcccctccct tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca
    44161 taaaaacact aattcagcgc tataacacct gaaaaaaggg cgatgaatct ttatatattc
    44221 tgaatattca cactctttac aggaactttt tagcgcaata gaccatcagg agtatagtga
    44281 tgcccgacag aagaagtgtt ctgaatgaaa gcgactaact taaggagtga ggaaagatgg
    44341 aaagtaattt cattgactgg catcccgctg acatcattgc aggtttgcgc aagaaaggaa
    44401 cgtcaatggc ggcggaatct cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat
    44461 tatcgcgccc atggccgaaa ggagagatga ttattgcgaa agccctggga actgacccct
    44521 gggttatctg gccatcacgc taccatgatc cgcagaccca tgagtttatc gacagaacgc
    44581 agttgatgcg tagctacact aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac
    44641 ggggctgcca gctctcagac gattactcgc ctttcacacg ctcaatattt gcacctaaag
    44701 cgcgcagttt gtcttcaatg cgttcgtagc cacgatcgat gtgataaata cgatcaacca
    44761 ccgtcgtccc ttccgcaata cagccagcca gcaccaggct tgctgatgca cgcagatcgg
    44821 ttgccataac ctgtgcacca gaaagttttt caacaccatg acaaataacg gtattgcttt
    44881 cgatttcggc gtgcgcgccc atacggctca gctctggcac atgcataaag cggttttcaa
    44941 agaccgtttc ggtgatgaac ccggtccctt cagccaccag gttcaacagc gtgaactggg
    45001 cctgcatatc ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca gccttcggac
    45061 gtttgccatg catatccagg ctaatccagt cttcgccgac ttcgatgtcc gctccagcat
    45121 cacgcagttt cgccagcacg gcgtccagag tatctggctg cgcgttacgg cagataattt
    45181 tgccacgaga gatcgccgcc gccaccagga aagtaccggt ttcgatacga tccggcagca
    45241 cgcgatagac accgccgcct aaacgttcca caccttcgat gacgatacga tcggtgccct
    45301 gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg atttccggtt
    45361 cacgcgctgc gttttcaata atcgtggtgc cttccgccag ggttgcagca cacatgatgg
    45421 tcaccgttgc gccaacgctg actttatcca tcacgatatg tgcgcctttc agacgaccat
    45481 cgacggaagc tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc
    45541 cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca accgccaggt agtgaaactt
    45601 gcccctgacc aaagcgcgct accagcggcc ccagcgccca gatagaagca cgcatggttt
    45661 taaccagatc gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata tgcacagaac
    45721 cattacgttc tactttcgca cccagctggc ttagcagttt cattgatgta tcgacgtctt
    45781 tcagtttcgg gacgttctgg atctctaccg gttcttccgc cagtagtgcg gcaaaaagaa
    45841 taggcagagc agcattttta gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc
    45901 cctgaacacg aaatttatcc atttagtttg ttctcagtta acaattcata tccgctaccg
    45961 gcgaatcgcc catagctcaa aagccgttca gtttgcgatc gcgcgcccac tccgcagggg
    46021 tatacgcttt gatcgacaca gcatgaatgc ggttatccgc aatatattcc atcagcgggc
    46081 catagaccgt ctgctgtttt ttaacccgac tcataccgtc aaacaactca cccacggcaa
    46141 taacctgaaa gtggctgcca tcgccggaaa cgtggacttc ctggagggag agagcgttca
    46201 tcaacacgct ctgaatttca ttattttcca tgggatcttc aatcatcagt taataaacca
    46261 gcgaaacatc ttagagcaaa gttgcgctgg cataaataag caaaaagcct cgctgataaa
    46321 tcagacaagg ctcgacttgc aggcaggttt gccggacagg cggttaacgc catatccggc
    46381 ctgaaaaaat ttaacgaggc agaacatcag caggcaaatt atacaatttc gcgagggtat
    46441 acactttgtc gtttaccccc tgaagcgtca cgttgttgcc ctgctttttc gccagatcga
    46501 taagatggag cagcagtgcc agtccccccg tatccacgcg ggacacacgg ctaagatcga
    46561 tgcaggtaat ccccttcacc gcctcctcac gcatttccca aagcggtagc agaacgtcct
    46621 gatccagctc tccggataac gccagcgtgt cacccgtctg catccagctc agtgactcgc
    46681 tcattatttt ttctcttcca gagtgatttt ctgttgagaa atcgatttca gttgcgcagt
    46741 caggccgtcg atacctttgg tacgcagcag cgttccccac tcgttttgtt tggtggtgat
    46801 catactgacg ccttcagcaa tcatgtcgta agcttgccag tttcccgtct gggagttttt
    46861 acgccactgg aagtccagac gcaccggcgg acggccattc gggtcaataa tggtaacgcg
    46921 aataggcaca atggttttat cgcccagcgg ctgttctggc gcaatctgat aggtttgacc
    46981 gtgatacatc gccagcgcct gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa
    47041 gtaggcttca cgttgagcag gggtcgcact cttgtaatac tggcccagca ccagcgcacc
    47101 ggcgtatttc acctgtacgt atggcagcag ttcctgatca acaatggtac gcagataatc
    47161 cgggttggcc cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc
    47221 ctcgtccatc agcttataag gattggtctg gtctgccgcg gttgccgcac tcagaggtgc
    47281 aatcaccagc aaagcgacca tcattaaacg tttaaacatg cgtcggttct cctgaaatta
    47341 tttcgttgta cccacaggtt cagtggtttc attattacct ggcgcagcag ctggcgcatc
    47401 gccactattc ttattgtcat cgcctttact accgtaaagg aactgaccaa tgagatcttc
    47461 cagcaccatc gcagacttgg tgtcctgaat tgtatcgcca tccttcagga tagcagtccc
    47521 cagttccggg tcttcaaaac cgacgtttaa tgccagatat tgttctccca gcaggccgga
    47581 agtacgaatg ctcagcgaac tggtatcagg aatgtggtta taacgttgtt caatttccag
    47641 cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata tccgccaccc ggcccacaac
    47701 aacaccacca atgctgaccg gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc
    47761 ataaagcgtg taggtcgatt cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac
    47821 aaacagcgcc gccagcagtg ctgctaataa aaagataccc acccaaattt catttttttt
    47881 cgtttgcatg aactcaattc ccaaacatca atgcggtcag cacaaaatcc agccccagaa
    47941 cagccagaga cgagtggaca acggtgcgag tggttgcccg gctaatcccg gcagacgtcg
    48001 ggatggcgtc gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc
    48061 tcttaatcag acagttgacc agatccatac gccagtcgac ggcattttgc attgccgacc
    48121 agaagaaccc gctatcaatg cctttccaac tgacgccgac cagcgatccg ccccagatcc
    48181 ccacggcgac gaagataacc gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg
    48241 gagaaataac ccgacgcagc ggatccaccg ccatcatctc catactggag agttgctctg
    48301 tagcgcgcat caggccgatt tctgcggtta acgccgaacc agcacgcccg gcaaacaaca
    48361 gcgcggcaac caccggcccc agttcacgca gtagcgataa cgccaccagc atacccagac
    48421 tggtttccgc gctataagtg gtcagaacca gataaccttg cagccccaac accattccga
    48481 tgaacacgcc agaaactaca ataatcagca tcgacaggac gccgacatta tagagctggc
    48541 gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca
    48601 ttaacccggc ccgcccgaac gttctcaggg ttttaatccc tttatgtccg agcgacgcca
    48661 gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg
    48721 gcaggatagc ggaacggaac aggcccgtca gctatcccgt ccagaaactg acgtacgcgc
    48781 ggatcaggat tcgtctgcaa cgcctgggca ctgccatgag cgacaatttt tttgtccgcc
    48841 aggatccagg cgtgatccgc aatacttaac acttccggca catcgtgaga aaccaccaca
    48901 caagtcacgc ccagcgcgct gttcagctca gaaatcagct tcaccagtac gcccatggtg
    48961 atgggatctt gcccaacaaa aggttcatca aacatgatga gatccggctc cagcgcaatc
    49021 gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg cattagttta
    49081 gccgctccac gcagccccac ggcctccagc ttcatcatca ccgtactatg caacaatggc
    49141 gcgggaagtt gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac gttcatatca
    49201 gtgaacaacg ccccggactg aaataacatg ctcatccgtt tacgcactgt atacaggcgc
    49261 gaacgagaca tcgccggaat attctcacca tcgaaaagga tctcaccatg atctggtgcg
    49321 atttgcccgc caatcagacg gagtagcgtc gttttaccga tgcccgatgg ccccatgatc
    49381 gccgtgatct tccctcgcgg cacggtcagg gaaatattat cgaagatgca gcgattgcca
    49441 cgcgtaaaac tgacatcgcg catatcgact aaattcgcca cagactgctc cataattcac
    49501 ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt taaccttgaa cccaacatat
    49561 ttacagaata ttacccgccg tggttagcga aagctggcat ttgttttact ttttagccgc
    49621 ataaagtcaa aattaagaat ccgttacggc tttctgaaaa tcttcagcgg accggcgagt
    49681 atacctgaag aaaggacgtt agatgctttt agctacggca ctgttaattg ttggtttact
    49741 tttggtcgtt tacagtgccg accgcctggt ttttgccgcg tctattcttt gccgaacctt
    49801 tggcatcccg ccgctgatca tcggcatgac ggtggtcagt attggtacat cgttaccaga
    49861 aatcatcgtc tcgcttgccg cgtctctgca cgaacaacgc gatttagccg ttggtacagc
    49921 cctcggctca aacattatca atatattgct gatcctcggt ctggctgcgc tggttcgtcc
    49981 ttttaccgtc cattccgatg ttctgcgccg tgaattaccc ttaatgttgt tggtcagcgt
    50041 ggtggccggt tccgtactct atgacggaca acttagtcga agcgatggta tctttctcct
    50101 ctttctggct gtgctatggc tgctgttcat tgttaaactt gcacgtcagg ggactgacag
    50161 cctgaccaga gagcagcttg cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt
    50221 atggctcggc attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc
    50281 cacggtgctg gcgaattact ttgccatcag cgagttgacg atggctctga cggcaattgc
    50341 tatcggaacc agtctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa
    50401 cgacattgct gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg
    50461 tttacccgcg ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag
    50521 cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc
    50581 gggccgtggt gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta
    50641 ctggttatcg ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac
    50701 aaccgggttt tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc
    50761 tggcggagct tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct
    50821 ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa
    50881 tggccgcaac gtttgccagc accggtacac cttcattttt cgtccatcct agtgaagccg
    50941 cgcatggtga tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag
    51001 gtgaatccag cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa
    51061 tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg
    51121 ttaaagtagc gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca
    51181 cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg
    51241 aagattttgc gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa
    51301 acgatattat gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg
    51361 acgcattgct ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata
    51421 tgatgattga aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg
    51481 atgttcgtca gttaagtatt gccgatgtga tgacgccggg gggaatacgt gtgcgccctg
    51541 gcattctggc cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg
    51601 ttgccgatgg cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg
    51661 tagtgtaaag attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg
    51721 ttacggccct gtcagcgccg acgttatggc aaaagcagag aacattcgtc tgctgatcct
    51781 cgacgttgat ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct
    51841 gaaagcgttc aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga
    51901 agtcgctatc attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg
    51961 gatcactcac ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg atttgctgga
    52021 aaaattggcg attgccccgg aaaatgtggc ttatgttggc gatgatctca tcgactggcc
    52081 ggtaatggaa aaagtgggtt taagcgtcgc cgtggccgat gcgcatccac tgttgatccc
    52141 gcgcgccgat tacgtgacgc gcattgctgg cggtcgtggc gcagtgcgcg aagtttgcga
    52201 cttattactc ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa
    52261 gccagacgtt gggttatcat tgtgctatca ctggcggttc tggtgatgat cggcattaat
    52321 atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa
    52381 agcgagcata cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt
    52441 gctcaacacg ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt
    52501 accacgtttg ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg
    52561 accaatgacc ggatgctcta tttatatgga cacgttgaag tcaacgcact tgtgccagac
    52621 tctcaacttc gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt
    52681 acctctgaag acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg
    52741 cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa
    52801 attcaaaaca aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag
    52861 cattccggca tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca
    52921 gcaatctctt gatatgcaag gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca
    52981 gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg
    53041 taaagaagtg attgacggat acggtaaacc ggcaacgttc taccagatgc aggacaacgg
    53101 taaacccgtt gaaggtcacg cttcccagat gcactacgaa ctggcaaaag attttgtcgt
    53161 tctgacgggt aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac
    53221 ttacctggtg aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac
    53281 cgttctggtg ccgtcgcagc tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa
    53341 gaagggtaat taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa
    53401 aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct
    53461 gctggggcca aacggtgccg gtaagaccac cactttctac atggttgtag gcattgtgcc
    53521 gcgcgatgcg ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc
    53581 acgcgcgcgc cgcggtatcg gctatctgcc acaggaagcc tccattttcc gtcgcctcag
    53641 cgtttacgat aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg
    53701 tgaagaccgc gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat
    53761 ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc
    53821 gaatccgaaa tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat
    53881 cgacattaaa cgcatcattg aacacctgcg cgacagcggc ctgggcgtgc tgatcactga
    53941 ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca
    54001 tttgatcgcc cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata
    54061 ccttggggaa gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt
    54121 acgattctga acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg
    54181 ccacagctcc aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag
    54241 ctacagcagg cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc
    54301 gacacccgcg aaacgcaaga cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa
    54361 gagatgccgg aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca
    54421 ccatccggca ccagcggtga ctacattgac gacgagctgc cggtctacca gggcgaaacg
    54481 acgcagacct tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact
    54541 gaccgcgcta ttgctacctc tatcgtcgat gccgttgatg aaaccggtta tctgactgtc
    54601 ccgctggaag atattctcga aagtatgggc gatgaagaga tcgacatcga cgaggttgaa
    54661 gccgtcctta agcggatcca acggtttgac ccggtcggtg tagcggcaaa agatctgcgt
    54721 gactgcctgc taatccaact ctcccaattc gataagacca cgccgtggct ggaagaggcc
    54781 agattgatca ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg
    54841 cgcgtcacgc gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc
    54901 gatccgcgcc ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg
    54961 ctggtgcgta agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt
    55021 ctgcaaatca accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc
    55081 cagtttatcc gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt
    55141 aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag
    55201 caaggtgaag aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg
    55261 catgaatcga cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt
    55321 tttgaactga agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc
    55381 tccacggcga ttcgtgcact ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg
    55441 ttgagcgaca gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc
    55501 actgttgcga agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc
    55561 gtttgaccca accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga
    55621 gatcaccgag gcactgcgcg aatttgttac agctaaattt gccaaacttg agcaatattt
    55681 tgaccgaatc aaccaagtct atgttgttct gaaagtggag aaagtcaccc acacctcaga
    55741 tgcaacactg catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta
    55801 cgctgccatt gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa
    55861 actgaaacaa cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca
    55921 caacgggcca tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt
    55981 cttaggtgaa attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag
    56041 ggaatgtacg cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag
    56101 cgagctggcg gcgaaacaac ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac
    56161 gcgggaaaaa atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact
    56221 tgaagaagat actctgcgtg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt
    56281 cgatgccatc gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca
    56341 aactaaaacg cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat
    56401 ctgccgccgt ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac
    56461 cgaaggtact ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga
    56521 gaaacagtac atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc
    56581 cctgcgtgcg ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt
    56641 acccgatctg gctcgaactc tggccgatcg cgagatttct gccgccgtca gcattgatgt
    56701 tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc
    56761 tttctcaccg caactactgt tcctggatgc cgaccgtaat accttaattc gtcgttacag
    56821 tgacacgcgc cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga
    56881 caaagaaagc gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc
    56941 agaaatgtcc gttcacgagc tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga
    57001 acgcgaactg accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc
    57061 agattacgtc tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc
    57121 aatgacaggt cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa
    57181 ttttatctac cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa
    57241 ccgtagctac ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat
    57301 tgcagagcaa ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg
    57361 tacgctggaa aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg
    57421 ggcatgcatg cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa
    57481 gtgctgttac gtaatgacga aggcaccgag gctgaagcca acagcgttat tgcgctgctg
    57541 atgttggatt ctgccaaagg acggcagatt gaagttgaag cgaccggtcc acaggaagag
    57601 gaagcactgg ccgccgttat cgccctcttt aattctggtt ttgatgaaga ttaatcttca
    57661 tcactttgac atacaactcc cttcaagctc ccccgacaat aagaaaatca cgtactgaaa
    57721 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    57781 agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat actggagatc
    57841 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    57901 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    57961 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc
    58021 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    58081 cggattgcgg aaccggttat gtctacgatg tgattgccga ttttgacggc aagccttcca
    58141 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    58201 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    58261 agccgcagaa tatcctctgt caccgcatca gtgagtcaga ggttatcccg gtcgtctgcg
    58321 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    58381 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    58441 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    58501 ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc
    58561 acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga agtggattag
    58621 gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    58681 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    58741 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    58801 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt tggccgtctg
    58861 ctgagaaatc gttgaagcac cgcgaatacg gttttcattg cgctcgttgt gcgccagggc
    58921 tttctcaatg gaagcgacat caaagcccca gtgctcagga aatttctgat cttctgcggc
    58981 aatcaccgcc agtcccatcc acggcgagat ttgatccata ctgacccagt cagaatgtgc
    59041 cacgtaacga aaattgccat gcagccaggc gctgacctgt cgctcgacca ttaccgctga
    59101 gaagggaaca ggcgcaacgc taaacaacgc gatgcccccg ccccagaaaa cggcgaggac
    59161 aaccattaac cgcaaaagaa aacggcgaac gaaactaaac accgttaagc ggcttttact
    59221 cattcagcca gaaccagcac gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca
    59281 atgttctgcg ccagcatata tgctggggtg gtgacaatct tattgtcttc atcaaccacg
    59341 atatcatcga caggacacgg cacatgctcc gcgcccatct cttccagcac ttctgcggta
    59401 tcgatatcag taccgatggt caaacgcagc gggaaatcga aaattttcgg cagcatcgcc
    59461 ggggcaatac acataaaacc aagcggtttt ccggcctgat gcatcgcttg tgccagcgcc
    59521 tttaattcac ggtcaacggt gcattcgcta ccaagactgg caaaattgct taaattcttc
    59581 gccgcgccaa acccccccgg cacaatcaac gcatccagtt cagcggcatc ggcctgggcc
    59641 agaggacgga tttcaccgcg cgttattcgt gccgcttcaa tcagcacatt gcgcgtttcc
    59701 gtcatcgctt cgccagttaa atggttgata acatcaacct gctgcttatc cggtgcaaag
    59761 cagaccgcct gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt
    59821 tcagaaccgt catagacgcc gcatccgctc agaattacgc caattttctt cattgtgatc
    59881 atcctttcgc aattgactga aacacattaa tttttttaat aaaaatggta cgcatcacac
    59941 atttaactga ttcatgtaac aaatcattta agttttgcta tcttaactgc gtgcggcctg
    60001 aaaaacagtg ctgtgccctt gtaactcatc ataataattt acggcgcagc caagatttcc
    60061 ctggtgttgg cgcagtattc gcgcaccccg gtctagccgg ggtcattttt tagtggcttt
    60121 tgccacccac gctttcagca cttctacgtc gtgacgccac tcttctttca tctcttcaat
    60181 ccattcaccg acgttatctt cccaggcggg aaggtcagga gactgaattt gctgacccag
    60241 ctgttgcaga tggcgtaacc ccactgaccc cgccgcacct ttaattttat gtccttcctc
    60301 aacaatgcct tttttatcct gcgccgtcag attcgactcc agcacgctga catagcccgg
    60361 catcatcttc tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta caagttcgag
    60421 atactgttcc agcatgggaa tatcgagcaa tgcttctgat ttactgttct cttctgtcgt
    60481 caccgtactc tcctcatcat cctgggtatc ccagaatttc ttgatcatcg cggttagcgc
    60541 cggaaccgaa agcggcttac tcagcacatc atccattcca gcattgaggt actcttgttt
    60601 gtctttcagc acgttagcgg ttaaggccac cagcggcggt aaatcctcgc gcggataacg
    60661 cttcgtcagt tcacgagaga tatccagccc ggtcatatct ggcaactgaa tatccagcaa
    60721 caccaggtcg tattcgcccg gtttaaacat ctccagcgcc gccttgccgg tcatggcgac
    60781 atcaacgctg ttacctaatt tttccagcac agaacgcgcg acaatcacgt tcagttcaat
    60841 gtcttccacc agcagcacat tcagcgccgg taaaggcata tcgtcttcat caaacgcatc
    60901 atcgacctct tctgccaccg acggtgcgtg gatcgtcaac gtaaaggttg aacctttgcc
    60961 ctgttcactg gtaaccgtaa tatcgccgcc catatttttc gccagacgac gagaaacggc
    61021 cagaccaata ccggtgccgg tggcaggttt accgccatga ctgtctttca cctggtaata
    61081 catggcgaag attttatcca gctcatcctg cggaatgccg ataccagaat cttccacttc
    61141 aaaatgcagc atatcgcctt catcgtagcg cacgcgcacg gtaacctggc cttgctgggt
    61201 gaatttgacg gcgttactga tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt
    61261 aatgacctga tgcggtaatg gcagcgtagg ctccaggtta aagcgcaatc ctttttgttg
    61321 cgcctgcaat gcggagagat tttccagatc ggcaaggaag ctggtgaaat caaccggctg
    61381 attatcaagc tggaccttgc gccgttccat cttatccatg tcgataatat cgttaaagat
    61441 attccccagc gtgacggccg aaacatggat agttttgaga tatttttcct gctcggcggt
    61501 gagttcggta tccagcagaa tgcggctcag gccgacgata ccgttcagcg gtgtacgcaa
    61561 ttcgtgactg atggtggaaa taaacgtcgt tttgtcgcgg ctggcgcgtt caagcgcatc
    61621 ctgataccgc ttacgctcgg taatgtcgcg accaaagccc atcaaaccgt gacgtttacc
    61681 cacgcggtcg tagtacggca ctttacggat ttcaaagcag gctttgcgcc cgtccgggta
    61741 atccagccac tgttcatagg tcagtgacac attatgacgg aacacttttt catcggtttc
    61801 aatgactttt gcggcggctt ccggtgagta aacatcagca ggtttcaggt gaaccagttg
    61861 tttttcgctt tttccggtca gcagctccat cgcgcggtta cagccggaaa actctttatc
    61921 ttcgttacga taaaaaacca agtcgggtga agcatcaagg aaggaacgta agaatgagga
    61981 ttgctgctcg agctgaattt gtgtctcttc gcgctctttg atttcaattt tcagttggcc
    62041 gaaggtttcc tgcagttctg cttccgcttt ttcacgaacg gcaatttcct gatttagctg
    62101 ggcaatatta tcttttaact gaacgttgag gctcaaatcg cgctcgcgca tctcctccag
    62161 tttttgcacc agccgtgaca gacgttgtcg tgactcctcc agttgctcga cgaccaccga
    62221 tagaaagtag accgcccacg gcgtaatcag caaaccaaag aagatagaac gaataacatc
    62281 aatgctttcg acctgaccat gcagcaccat ggttaccgcc atttgtacca caatggcaag
    62341 aacgacgagg gccagcgcca gcaacattga gaagcgcacc agacctaact tcatcatcag
    62401 gtcaacataa tactgcgcca gcagacgaat ttgcttcatt agggaattcc ttcacgacaa
    62461 cctgccccaa taatacccaa ttctgacagt tacgttataa attgtgtgag aaatgcggaa
    62521 ttcccctcaa cgacctactc cgttggaggt agccacggac gtcccagcgc cgatccctgt
    62581 acgccatgtt cattcagata gcgatccagc tcgaccattc ccgtccatcg attttcgcac
    62641 cacagcggag caagcagcgt cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact
    62701 tccggtggcg tatggcgaat catttctccg gcagtgagcg tataatcctc cagttcaata
    62761 ccgttcaaac gccccgcttc ccaggctttc gccataatgc tgcctttcac aatatgcagc
    62821 ggatgcagct ttatgccgtc cacgctggtt tcaaccactc gctccagcgt ttgcaaacat
    62881 tcagcctgcc cttcgccagg caggccgaca attaagtggg aacacacctt cagcccgcgc
    62941 tgacgtgcca gctgggttgt acgctgataa caggcaaaat catggccgcg gttgatgcga
    63001 tgcagtgttt tgtcgtgggc ggtttgtagc cccagctcca gccacacttc gtaaccctgg
    63061 tccttatatt cgcaaagcag atccagcacc acatccggca cgcagtccgg gcgggtacca
    63121 acacacaaac cgacaatatt ggcctggctc accgcctgct gatacataga acgcagcacc
    63181 tgaacttccg caaaggtgct ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg
    63241 ttaactaaat tcgcctggtg cgccagttgc tcggcaatgg aacgatgctg ctgcgcttca
    63301 tcggcaaacg aggcaacatt acagaatgtg cagccaccac gcccgatggt accgtcacgg
    63361 ttagggcagc taaaaccgcc gtgcagcgtc agcttatgca ccttttgccc ataacgacgg
    63421 gtgagatcac caccaaacat attgactaat ttctgtaact gcataatctg atagaccgcg
    63481 ccttgaaaag aggccaaagc ctgccatttt tagctcaatt cggcgatgac ctgggtcaat
    63541 cgtcccggcc tgcttttatc aactgcataa tcaatcaaaa ttaccgaaat ttcatgcaca
    63601 atcacataaa tcacttttgc ttatcttgtg tcagattttt ttatctcctt atggatttta
    63661 ggcaaaaaca gtagcatgaa acgtcattac caattaaggc agtataaaat gctggttttc
    63721 tcgtcagttc aaggcaggat aagggttaac acgcctttat gacagtcagg aattgactgt
    63781 ttctctaacg actctccttt tagccttaaa gataaaatct attttaattt cagctagtta
    63841 ataaagaatt ttgcgctaaa gcacatttct gtaccaataa gcttgccatt tgacctgtat
    63901 cagctttccc gataagttgg aaatccgctg gaagctttct ggatgagcag cctgctcatc
    63961 atatttatgc agtaattgag atcccctctt caccgtatta accgatgcga aaaggacaac
    64021 aagggggcga atgcgaggcg cgcgtatgac acgcaaaccc cgtcgccacg ctctttctgt
    64081 gcccgtgcgc agcggttcgg aagtggggtt cccgcagagc ctgggggagg ttcacgatat
    64141 gttgtacgat aaatcccttg agagggataa ctgtggtttc ggcctgatcg cccacataga
    64201 aggcgaacct agccacaagg tagtgcgtac tgcaatacac gcactggccc gcatgcagca
    64261 ccgtggcgcg attctcgccg atggtaaaac cggcgacggt tgcggcttgc tgttacaaaa
    64321 accggatcgc ttttttcgca tcgttgcgca ggagcgcggc tggcgtttag caaaaaacta
    64381 cgctgtcggg atgctcttcc tgaataaaga tcctgaactc gccgctgccg cacgccgcat
    64441 cgttgaagaa gaactgcaac gcgaaacctt gtcgattgtg ggctggcgtg atgtccccac
    64501 taacgaaggc gtgctgggtg aaatcgccct ctcctctctg ccacgcattg agcaaatttt
    64561 tgtgaacgcc ccggcaggct ggcgtccacg cgatatggag cgccgtctgt ttatcgcccg
    64621 ccgccgcatt gaaaagcgtc tcgaagccga caaagacttc tacgtctgta gcctgtcgaa
    64681 tctggtgaac atctataaag gtctgtgtat gccggcggat ctgccgcgct tttatctgga
    64741 tcttgcggac ctgcgtctgg aatcggccat ttgcctgttc caccagcgct tctccactaa
    64801 caccgtaccg cgctggccgc tggcgcaacc gttccgctat ctggcgcata acggtgaaat
    64861 caacaccatc accggtaacc gccaatgggc gcgtgcgcgt acctataaat tccagacgcc
    64921 gctgatccct gacctgcacg acgccgcgcc attcgtcaac gaaaccggtt ctgactccag
    64981 ttccatggat aacatgctgg aactgctgct ggcaggcggg atggatatca tccgcgccat
    65041 gcgcctgtta gtgccacccg cctggcagaa caacccggat atggacccgg aactgcgtgc
    65101 cttctttgac tttaactcca tgcatatgga gccgtgggat ggtccggcgg gcatcgtgat
    65161 gtccgatggt cgcttcgctg cctgtaacct cgaccgtaac ggtctgcgtc cggcacgcta
    65221 cgtcattacc aaagacaagc tcatcacctg cgcctctgaa gtcggtatct gggattacca
    65281 gcctgacgaa gtggtcgaaa aaggccgcgt cgggccaggc gaactgatgg tgatcgacac
    65341 tcgcagtggg cgtattctgc actcggcaga aaccgatgac gatctgaaaa gccgccaccc
    65401 ttataaagag tggatggaga aaaacgtccg ccgactggta ccgtttgaag atctgcccga
    65461 tgaagaagtg ggtagccgcg aactggacga cgacacgctt gccagctacc agaaacagtt
    65521 taactacagc gcggaagagc tggactccgt aattcgcgta ctgggcgaaa acggtcagga
    65581 agcggtcggt tcgatgggcg atgatacccc attcgccgtg ctctccagtc agccgcgcat
    65641 tatttacgac tacttccgcc agcagtttgc ccaggtgact aacccgccaa tcgacccgct
    65701 gcgtgaagcg catgttatgt cgctcgccac cagtatcggt cgtgaaatga acgtcttttg
    65761 cgaagcagag ggccaggcgc accgtttaag ctttaaatcg ccgattctgc tctactccga
    65821 tttcaaacag ctcacgacga tgaaagagga gcactaccgc gcagatacgc tggatatcac
    65881 ctttgacgtc actaaaacca cgctcgaagc gacagtcaaa gagctatgcg acaaagccga
    65941 aaaaatggta cgtagcggca ccgtgttgct ggtgctctcc gaccggaata tcgctaaaga
    66001 tcgcctgccg gttccggctc cgatggcggt tggcgcgatc cagacccgtc tggtcgatca
    66061 aagcctgcgt tgcgatgcca acatcatcgt cgaaaccgcc agcgcccgcg atccgcacca
    66121 cttcgccgtg ttgctgggtt tcggcgcgac ggctatttat ccgtaccttg cctatgaaac
    66181 gctgggccgc ctggtagaca cccacgcgat tgccaaagat tatcgtaccg tgatgctcaa
    66241 ctaccgtaac ggcatcaaca aaggcttgta caaaatcatg tccaaaatgg gcatctccac
    66301 catcgcctct taccgctgct cgaaactgtt tgaagcggtc ggtctacacg atgatgtagt
    66361 gggcctgtgc ttccagggcg cggtcagccg cattggtggg gcaagctttg aagacttcca
    66421 gcaggatctg ctgaatctgt cgaaacgtgc ctggctggcg cgcaagccga ttagccaggg
    66481 cggcctgctg aaatacgtcc acggcggcga ataccacgcc tacaacccgg acgtggtgcg
    66541 cacgctgcaa caggcggtac aaagcggcga gtacagcgac tatcaggaat acgcgaagct
    66601 ggttaatgag cgtccggcaa ccacgctgcg cgatctgctg gcaattacgc cgggtgaaaa
    66661 cgcggtcaac attgctgatg ttgaaccggc aagcgaactg tttaaacgct ttgataccgc
    66721 cgcgatgtct atcggcgcgt taagcccgga agcccacgag gcgctggcgg aagcgatgaa
    66781 tagcatcggc ggtaactcga actccggtga aggcggcgaa gacccggcgc gctatggcac
    66841 taacaaagtg tcgcgcatca agcaggtggc ttctggtcgc ttcggcgtta ctccggcgta
    66901 tctggtcaat gccgacgtca ttcagattaa agtcgcccag ggcgcgaagc cgggcgaagg
    66961 tggtcagttg ccgggcgaca aagtcactcc ttacatcgcc aaactgcgct attcggtgcc
    67021 cggagtgacg ctgatctccc cgccgccgca ccacgatatc tactctatcg aagacttagc
    67081 gcagcttatt ttcgacctca agcaggttaa cccgaaagcg atgatctccg ttaagctggt
    67141 gtccgaacca ggcgttggca ccatcgcgac tggcgtggcg aaagcttatg cggacttgat
    67201 caccatcgcg ggctatgacg gcggcaccgg cgcaagcccg ctgtcgtcag tgaaatatgc
    67261 aggctgcccg tgggaactcg gtctggtgga aacccaacag gcgctggttg ccaacggctt
    67321 gcgtcataaa attcgtttgc aggttgatgg cggcctgaaa acgggtgtcg atatcatcaa
    67381 agcggcgatt ctcggcgcag aaagcttcgg cttcggcact ggcccaatgg tggcgctcgg
    67441 ctgtaaatat ctgcgtattt gccacctgaa caactgcgcc acgggtgtag caactcagga
    67501 tgacaaactg cgtaagaatc actatcacgg cctgccgttc aaggtgacta attactttga
    67561 gtttatcgcc cgtgaaaccc gcgagctgat ggcacagctg ggagtaacgc gtctggtgga
    67621 tctgattggt cgcaccgacc tgctgaaaga gctggacggt ttcaccgcca aacagcagaa
    67681 actggcgctg tcgaagctgc tggagactgc cgagccacat gcaggtaagt cactctactg
    67741 cactgaaaac aacccgccgt ttgataacgg cctactgaac gcgcagttgc tgcaacaggc
    67801 gaaaccgttt gtcgatgaac gccagagcaa aaccttctgg ttcgatatcc gcaacaccga
    67861 ccgttctgtc ggcgcgtcgc tttcaggcta tatcgcccag acgcacggcg atcaggggct
    67921 ggcagccgat cctatcaaag cgtacttcaa cggcaccgca ggccagagtt tcggtgtgtg
    67981 gaacgcgggc ggcgtggaac tgtacctgac cggggatgcc aacgactatg tcggtaaagg
    68041 catggcgggc ggcttaatcg ccattcgtcc tccggttggt tccgccttcc gcagccatga
    68101 agcaagcatt atcggcaaca cctgcctgta tggcgcgacc ggtggtcgtc tgtatgccgc
    68161 aggccgcgcg ggtgaacgtt tcggcgtgcg gaactccggt gctatcaccg tggtagaagg
    68221 cattggcgac aacggctgtg aatatatgac gggcggtatt gtctgtattc tgggtaaaac
    68281 cggcgttaac ttcggcgcgg gcatgaccgg cggcttcgct tacgttctcg atgaaagcgg
    68341 tgatttccgc aaacgcgtta acccggaact ggtcgaagtc ttaagcgtcg acgatctggc
    68401 gatccatgaa gagcatctgc gcggtcttat caccgagcat gttcagcata ccggttctca
    68461 gcgcggtgaa gagattctgg cgaactggtc aaccttcgcc acgaaattcg cgctggttaa
    68521 accgaagtcc agtgatgtaa aagcactgct gggtcaccgt agtcgtagcg cagcagagtt
    68581 gcgcgtgcag gcgcagtaag gggtagcaac aatgagtcag aatgtttatc aatttatcga
    68641 cctgcagcgc gttgatccgc caaagaaacc gctgaagatc cgtaaaattg agtttgttga
    68701 aatttacgag ccgttttccg aaggccaggc caaagcgcag gctgaccgct gtctgtcgtg
    68761 cggcaaccca tactgcgagt ggaaatgccc ggtacacaac tacatcccaa actggctgaa
    68821 gctcgccaac gaggggcgta tttttgaagc ggcagaactg tcgcatcaga ccaacacact
    68881 gccggaagta tgcggacgtg tctgcccgca agaccgtctg tgcgaaggtt cctgcaccct
    68941 gaacgatgag ttcggtgcgg tgaccatcgg caacattgag cgttatatca atgataaagc
    69001 gttcgaaatg ggctggcgtc cggatatgtc cggcgtgaag caaaccggta aaaaagtggc
    69061 gattatcggt gcaggcccgg caggtctggc gtgtgcggat gtcctgacgc gtaacggcgt
    69121 aaaagccgtt gtcttcgacc gtcatccaga aattggcggg ctgctgacct tcggtattcc
    69181 ggccttcaag ctggaaaaag aggtaatgac gcgtcgccgt gaaatcttca ccggcatggg
    69241 tattgaattc aaactcaata ccgaagtggg ccgcgacgta cagctggacg atctgctgag
    69301 tgattacgat gccgtgttcc ttggcgttgg gacttatcag tcaatgcgcg gcgggctgga
    69361 aaacgaagac gccgatggcg tgtacgcagc gctgccgttc cttatcgcca acaccaaaca
    69421 gttaatgggc tttggtgaaa cccgcgacga accgttcgtc agcatggaag gcaaacgcgt
    69481 ggtagtactt ggcggtggcg acactgcgat ggactgcgtg cgcacgtctg tacgccaggg
    69541 ggcaaagcac gttacctgtg cctatcgtcg tgatgaagag aacatgccgg gttcccgccg
    69601 cgaagtgaaa aacgcgcggg aagaaggcgt agagttcaaa ttcaacgtcc agccactggg
    69661 gattgaagtg aacggtaacg gcaaagtcag cggcgtaaaa atggtacgta ccgaaatggg
    69721 cgaaccggat gccaaaggcc gtcgccgcgc ggagatcgtt gcaggttccg aacatatcgt
    69781 tccggcagat gcggtgatca tggcgtttgg tttccgtcca cacaacatgg aatggctggc
    69841 aaaacacagc gtcgagctgg attcacaagg ccgcatcatc gctccggaag gcaacgacaa
    69901 cgctttccag accagcaacc cgaaaatctt tgctggcggc gatatcgtcc gtggttccga
    69961 tctggtcgtt accgccatcg ccgaaggtcg caaagcggca gacggcatta tgaactggct
    70021 ggaagtttaa gctctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc
    70081 ctttctttct ggaaatcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg
    70141 gattgttacc ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat
    70201 aaagggacag ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca
    70261 caacggcatt attcatctgg tcgatagcgc ccgaacggaa acggtacgta gcgttaacgc
    70321 gctgatgacc gcgacgtact gggaaattgg ccgacggatt gtcgaatttg aacaaggtgg
    70381 tgaggccaga gcagcttatg gtgcacagtt gattgatcgg ctatcgaagg atttaagtcg
    70441 acggtataaa cgtggggtct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt
    70501 ttttcaacat gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc
    70561 gcagacacca tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatt
    70621 gagaggctgt ttgatatttc cacagctttt gtattgcatc tcctattttc ggtttgtata
    70681 ttcatgcaaa taaacgaag
//
LOCUS       DAECOD010000005        67496 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.005, whole genome shotgun sequence.
ACCESSION   DAECOD010000005 DAECOD010000000
VERSION     DAECOD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.005"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..228
                     /locus_tag="KKO15_000316"
     CDS             <1..228
                     /locus_tag="KKO15_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBD6359249.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="KKO15_000317"
                     /pseudo
     CDS             648..1263
                     /locus_tag="KKO15_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="KKO15_000318"
     CDS             1366..1689
                     /locus_tag="KKO15_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBD6359250.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="KKO15_000319"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="KKO15_000319"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBD6359251.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="KKO15_000320"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="KKO15_000320"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBD6359252.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="KKO15_000321"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="KKO15_000321"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBD6359253.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="KKO15_000322"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="KKO15_000322"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBD6359254.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="KKO15_000323"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="KKO15_000323"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBD6359255.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="KKO15_000324"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="KKO15_000324"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBD6359256.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="KKO15_000325"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="KKO15_000325"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBD6359257.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="KKO15_000326"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="KKO15_000326"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBD6359258.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSLMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="KKO15_000327"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="KKO15_000327"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBD6359259.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="KKO15_000328"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="KKO15_000328"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBD6359260.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="KKO15_000329"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="KKO15_000329"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBD6359261.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="KKO15_000330"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="KKO15_000330"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBD6359262.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="KKO15_000331"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="KKO15_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBD6359263.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="KKO15_000332"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="KKO15_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBD6359264.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="KKO15_000333"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="KKO15_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBD6359265.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="KKO15_000334"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="KKO15_000334"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBD6359266.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="KKO15_000335"
     CDS             complement(21228..23060)
                     /locus_tag="KKO15_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBD6359267.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="KKO15_000336"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="KKO15_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBD6359268.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="KKO15_000337"
     CDS             complement(23808..24302)
                     /locus_tag="KKO15_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBD6359269.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="KKO15_000338"
     CDS             complement(24385..24840)
                     /locus_tag="KKO15_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBD6359270.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="KKO15_000339"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="KKO15_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBD6359271.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="KKO15_000340"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="KKO15_000340"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD6359272.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKVVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="KKO15_000341"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="KKO15_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBD6359273.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="KKO15_000342"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="KKO15_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBD6359274.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="KKO15_000343"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="KKO15_000343"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBD6359275.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="KKO15_000344"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="KKO15_000344"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBD6359276.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="KKO15_000345"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="KKO15_000345"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBD6359277.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPPDGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="KKO15_000346"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="KKO15_000346"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBD6359278.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="KKO15_000347"
     CDS             33416..34309
                     /locus_tag="KKO15_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBD6359279.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="KKO15_000348"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="KKO15_000348"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBD6359280.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="KKO15_000349"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="KKO15_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBD6359281.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="KKO15_000350"
     CDS             complement(36225..36941)
                     /locus_tag="KKO15_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6359282.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTVLAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="KKO15_000351"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="KKO15_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBD6359283.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="KKO15_000352"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="KKO15_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBD6359284.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="KKO15_000353"
                     /pseudo
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="KKO15_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="KKO15_000354"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="KKO15_000354"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBD6359285.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="KKO15_000355"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="KKO15_000355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBD6359286.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="KKO15_000356"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="KKO15_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBD6359287.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="KKO15_000357"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="KKO15_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBD6359288.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="KKO15_000358"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="KKO15_000358"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBD6359289.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="KKO15_000359"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="KKO15_000359"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBD6359290.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="KKO15_000360"
     CDS             complement(45790..46449)
                     /locus_tag="KKO15_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6359291.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="KKO15_000361"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="KKO15_000361"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBD6359292.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="KKO15_000362"
     CDS             complement(47344..48357)
                     /locus_tag="KKO15_000362"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD6359293.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="KKO15_000363"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="KKO15_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBD6359294.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="KKO15_000364"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="KKO15_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBD6359295.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="KKO15_000365"
     CDS             complement(50650..51828)
                     /locus_tag="KKO15_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359296.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="KKO15_000366"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="KKO15_000366"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBD6359297.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="KKO15_000367"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="KKO15_000367"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBD6359298.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="KKO15_000368"
     CDS             complement(55610..55888)
                     /locus_tag="KKO15_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBD6359299.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="KKO15_000369"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="KKO15_000369"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBD6359300.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="KKO15_000370"
     CDS             complement(56470..57279)
                     /locus_tag="KKO15_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBD6359301.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="KKO15_000371"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="KKO15_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBD6359302.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="KKO15_000372"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="KKO15_000372"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBD6359303.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="KKO15_000373"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="KKO15_000373"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD6359304.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="KKO15_000374"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="KKO15_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBD6359305.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMYGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="KKO15_000375"
     CDS             complement(60980..61870)
                     /locus_tag="KKO15_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBD6359306.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="KKO15_000376"
     CDS             complement(61857..62381)
                     /locus_tag="KKO15_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6359307.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="KKO15_000377"
     CDS             complement(62378..62848)
                     /locus_tag="KKO15_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6359308.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="KKO15_000378"
     CDS             complement(62845..63393)
                     /locus_tag="KKO15_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6359309.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="KKO15_000379"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="KKO15_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="KKO15_000380"
     CDS             complement(64140..64862)
                     /locus_tag="KKO15_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD6359310.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66781)
                     /locus_tag="KKO15_000381"
     CDS             complement(64922..66781)
                     /locus_tag="KKO15_000381"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HBD6359311.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(66863..67426)
                     /locus_tag="KKO15_000382"
     CDS             complement(66863..67426)
                     /locus_tag="KKO15_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6359312.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16861 a        18259 c        16746 g        15630 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgagaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca taaggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcaac
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtagcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ttcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaagtcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctcc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tgtaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcaca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgcatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acctaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc taggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaaact tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattaaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 aaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgtatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattggc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcacgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaat
//
LOCUS       DAECOD010000006        66642 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.006, whole genome shotgun sequence.
ACCESSION   DAECOD010000006 DAECOD010000000
VERSION     DAECOD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.006"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="KKO15_000383"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="KKO15_000383"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBD6359313.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="KKO15_000384"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="KKO15_000384"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBD6359314.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="KKO15_000385"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="KKO15_000385"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBD6359315.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="KKO15_000386"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="KKO15_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBD6359316.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="KKO15_000387"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="KKO15_000387"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBD6359317.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="KKO15_000388"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="KKO15_000388"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD6359318.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="KKO15_000389"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="KKO15_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBD6359319.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="KKO15_000390"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="KKO15_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD6359320.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="KKO15_000391"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="KKO15_000391"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD6359321.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="KKO15_000392"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="KKO15_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBD6359322.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="KKO15_000393"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="KKO15_000393"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBD6359323.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="KKO15_000394"
     CDS             complement(15666..16703)
                     /locus_tag="KKO15_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6359324.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="KKO15_000395"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="KKO15_000395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6359325.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="KKO15_000396"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="KKO15_000396"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBD6359326.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="KKO15_000397"
     CDS             19445..19885
                     /locus_tag="KKO15_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBD6359327.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="KKO15_000398"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="KKO15_000398"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD6359328.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="KKO15_000399"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="KKO15_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="KKO15_000400"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="KKO15_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBD6359329.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="KKO15_000401"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="KKO15_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBD6359330.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="KKO15_000402"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="KKO15_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBD6359331.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="KKO15_000403"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="KKO15_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBD6359332.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="KKO15_000404"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="KKO15_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBD6359333.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="KKO15_000405"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="KKO15_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBD6359334.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="KKO15_000406"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="KKO15_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBD6359335.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="KKO15_000407"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="KKO15_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBD6359336.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="KKO15_000408"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="KKO15_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBD6359337.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="KKO15_000409"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="KKO15_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBD6359338.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="KKO15_000410"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="KKO15_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBD6359339.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="KKO15_000411"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="KKO15_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBD6359340.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="KKO15_000412"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="KKO15_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBD6359341.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="KKO15_000413"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="KKO15_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBD6359342.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="KKO15_000414"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="KKO15_000414"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBD6359343.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="KKO15_000415"
     CDS             37413..37682
                     /locus_tag="KKO15_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBD6359344.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="KKO15_000416"
     CDS             complement(37685..38044)
                     /locus_tag="KKO15_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBD6359345.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="KKO15_000417"
     CDS             38185..38811
                     /locus_tag="KKO15_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBD6359346.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="KKO15_000418"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="KKO15_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBD6359347.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="KKO15_000419"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="KKO15_000419"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBD6359348.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="KKO15_000420"
     CDS             41651..42316
                     /locus_tag="KKO15_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBD6359349.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="KKO15_000421"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="KKO15_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBD6359350.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="KKO15_000422"
     CDS             complement(42950..44167)
                     /locus_tag="KKO15_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359351.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="KKO15_000423"
     CDS             44301..45350
                     /locus_tag="KKO15_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD6359352.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="KKO15_000424"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="KKO15_000424"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBD6359353.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="KKO15_000425"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="KKO15_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBD6359354.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="KKO15_000426"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="KKO15_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBD6359355.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="KKO15_000427"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="KKO15_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBD6359356.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="KKO15_000428"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="KKO15_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBD6359357.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="KKO15_000429"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="KKO15_000429"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBD6359358.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="KKO15_000430"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="KKO15_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBD6359359.1"
                     /translation="MQRVTITLDDDLLKTLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="KKO15_000431"
     CDS             complement(51545..51904)
                     /locus_tag="KKO15_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBD6359360.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="KKO15_000432"
     CDS             complement(51901..52176)
                     /locus_tag="KKO15_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBD6359361.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="KKO15_000433"
     CDS             complement(52235..53359)
                     /locus_tag="KKO15_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6359362.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="KKO15_000434"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="KKO15_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBD6359363.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="KKO15_000435"
     CDS             complement(56091..57158)
                     /locus_tag="KKO15_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6359364.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="KKO15_000436"
     CDS             complement(57524..59146)
                     /locus_tag="KKO15_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD6359365.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="KKO15_000437"
     CDS             complement(59408..60997)
                     /locus_tag="KKO15_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD6359366.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="KKO15_000438"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="KKO15_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="KKO15_000439"
     CDS             complement(62499..62975)
                     /locus_tag="KKO15_000439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359367.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="KKO15_000440"
     CDS             complement(62968..63732)
                     /locus_tag="KKO15_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD6359368.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="KKO15_000441"
     CDS             63978..64739
                     /locus_tag="KKO15_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD6359369.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCPLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="KKO15_000442"
     CDS             complement(64846..65346)
                     /locus_tag="KKO15_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359370.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65497..>66642)
                     /locus_tag="KKO15_000443"
     CDS             complement(65497..>66642)
                     /locus_tag="KKO15_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6359371.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPRATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16187 a        17893 c        16354 g        16208 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgca gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcagatg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaacg gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcaacac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtaccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg ggagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaagatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttatgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcaggttaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctggcgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtgc attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgc caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actgaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cgcgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc gcgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tgaagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcact tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggccgga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaagat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaacatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgtcctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgatgcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gccttccgca ctcaagccag aaacataagc
    65641 aataccatct tcacctacaa tgccggccat attttcagtg tctttgctat aactaatcac
    65701 agcgccgaag gggacgggtt taccctcttt ctgttttaag gttactaatc ctttatcccc
    65761 gctcttcact gccaatgtcg caggcacaac ggctccagct gtcggtacaa ccaattgact
    65821 agtttgtggt aagtcgtaat tcttacctat cgttttttca tccagaataa catcgtctct
    65881 tcggtatggc tgcactggca gtattgcata cccacgaaaa tcagtactca gtcccggcca
    65941 gccattaacg ttaacccccg ttgctctggg tgcctctata agggctaatg tcccatccac
    66001 attttgacca aatgtaagtc catcccttgt aataagtatc ccaccgctta aatttgcacc
    66061 tagctgtttc atgttttcgc tggagctgta atggctacta aactgaccat atgatccatt
    66121 tttgccaact tgcacaatgt tcatcgtgct cttcggttgt gactgatgag cgttagttat
    66181 atttgtcgct acatcccacc atacaccacc actattctga cctgataatg aaacctgata
    66241 gttattaccc tgctctcccg aacgactcat ctgtgaagac gcataaattt gattttcgtc
    66301 acccagcgat aatggcactg aaatacccac gcttaatttc cgatctactt tccatttatg
    66361 cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat gttatgcctc tccataccgt
    66421 actataactc aggctggtag accgcgtgtt tcctgtgccc cgggaagaat tatacctcgt
    66481 ggtattgaag ttcaaattcc cgatattacc aaggggctgg gataaggata tcgtagagcc
    66541 atcgtgacta ttcttatctc tttttctgtt ccgttcatcc tgtgcactta ctgcttcagt
    66601 cagtgtggca taacctgatg cataacggtt cgtggttata ga
//
LOCUS       DAECOD010000007        65976 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.007, whole genome shotgun sequence.
ACCESSION   DAECOD010000007 DAECOD010000000
VERSION     DAECOD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.007"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(82..561)
                     /gene="folK"
                     /locus_tag="KKO15_000444"
     CDS             complement(82..561)
                     /gene="folK"
                     /locus_tag="KKO15_000444"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBD6359372.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(558..1976)
                     /gene="pcnB"
                     /locus_tag="KKO15_000445"
     CDS             complement(558..1976)
                     /gene="pcnB"
                     /locus_tag="KKO15_000445"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBD6359373.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2015..2941)
                     /gene="gluQRS"
                     /locus_tag="KKO15_000446"
     CDS             complement(2015..2941)
                     /gene="gluQRS"
                     /locus_tag="KKO15_000446"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBD6359374.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2978..3433)
                     /gene="dksA"
                     /locus_tag="KKO15_000447"
     CDS             complement(2978..3433)
                     /gene="dksA"
                     /locus_tag="KKO15_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBD6359375.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3611..4315)
                     /gene="sfsA"
                     /locus_tag="KKO15_000448"
     CDS             complement(3611..4315)
                     /gene="sfsA"
                     /locus_tag="KKO15_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBD6359376.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4330..4860)
                     /gene="thpR"
                     /locus_tag="KKO15_000449"
     CDS             complement(4330..4860)
                     /gene="thpR"
                     /locus_tag="KKO15_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBD6359377.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4934..7363
                     /gene="hrpB"
                     /locus_tag="KKO15_000450"
     CDS             4934..7363
                     /gene="hrpB"
                     /locus_tag="KKO15_000450"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBD6359378.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7559..10093
                     /gene="mrcB"
                     /locus_tag="KKO15_000451"
     CDS             7559..10093
                     /gene="mrcB"
                     /locus_tag="KKO15_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBD6359379.1"
                     /translation="MAGNDREPIGRKGKLTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10313..12556
                     /gene="fhuA"
                     /locus_tag="KKO15_000452"
     CDS             10313..12556
                     /gene="fhuA"
                     /locus_tag="KKO15_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBD6359380.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12607..13404
                     /gene="fhuC"
                     /locus_tag="KKO15_000453"
     CDS             12607..13404
                     /gene="fhuC"
                     /locus_tag="KKO15_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBD6359381.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13404..14294
                     /gene="fhuD"
                     /locus_tag="KKO15_000454"
     CDS             13404..14294
                     /gene="fhuD"
                     /locus_tag="KKO15_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBD6359382.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELMTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14291..16273
                     /gene="fhuB"
                     /locus_tag="KKO15_000455"
     CDS             14291..16273
                     /gene="fhuB"
                     /locus_tag="KKO15_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBD6359383.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16308..17588)
                     /gene="hemL"
                     /locus_tag="KKO15_000456"
     CDS             complement(16308..17588)
                     /gene="hemL"
                     /locus_tag="KKO15_000456"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBD6359384.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17813..19234
                     /gene="clcA"
                     /locus_tag="KKO15_000457"
     CDS             17813..19234
                     /gene="clcA"
                     /locus_tag="KKO15_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBD6359385.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19258..19323
                     /locus_tag="KKO15_000458"
     CDS             19258..19323
                     /locus_tag="KKO15_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359386.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19316..19660
                     /gene="erpA"
                     /locus_tag="KKO15_000459"
     CDS             19316..19660
                     /gene="erpA"
                     /locus_tag="KKO15_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBD6359387.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19707..20330)
                     /locus_tag="KKO15_000460"
     CDS             complement(19707..20330)
                     /locus_tag="KKO15_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBD6359388.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20368..21168)
                     /gene="btuF"
                     /locus_tag="KKO15_000461"
     CDS             complement(20368..21168)
                     /gene="btuF"
                     /locus_tag="KKO15_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBD6359389.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21161..21859)
                     /gene="mtnN"
                     /locus_tag="KKO15_000462"
     CDS             complement(21161..21859)
                     /gene="mtnN"
                     /locus_tag="KKO15_000462"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBD6359390.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21943..23460
                     /gene="dgt"
                     /locus_tag="KKO15_000463"
     CDS             21943..23460
                     /gene="dgt"
                     /locus_tag="KKO15_000463"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD6359391.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23590..25014
                     /gene="degP"
                     /locus_tag="KKO15_000464"
     CDS             23590..25014
                     /gene="degP"
                     /locus_tag="KKO15_000464"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBD6359392.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25169..26325
                     /gene="cdaR"
                     /locus_tag="KKO15_000465"
                     /pseudo
     CDS             25169..26325
                     /gene="cdaR"
                     /locus_tag="KKO15_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26414..26800)
                     /locus_tag="KKO15_000466"
     CDS             complement(26414..26800)
                     /locus_tag="KKO15_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBD6359393.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27114..27938)
                     /gene="dapD"
                     /locus_tag="KKO15_000467"
     CDS             complement(27114..27938)
                     /gene="dapD"
                     /locus_tag="KKO15_000467"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBD6359394.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27969..30641)
                     /gene="glnD"
                     /locus_tag="KKO15_000468"
     CDS             complement(27969..30641)
                     /gene="glnD"
                     /locus_tag="KKO15_000468"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBD6359395.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVHQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30703..31497)
                     /gene="map"
                     /locus_tag="KKO15_000469"
     CDS             complement(30703..31497)
                     /gene="map"
                     /locus_tag="KKO15_000469"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBD6359396.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31865..32590
                     /gene="rpsB"
                     /locus_tag="KKO15_000470"
     CDS             31865..32590
                     /gene="rpsB"
                     /locus_tag="KKO15_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBD6359397.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32848..33699
                     /gene="tsf"
                     /locus_tag="KKO15_000471"
     CDS             32848..33699
                     /gene="tsf"
                     /locus_tag="KKO15_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBD6359398.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33846..34571
                     /gene="pyrH"
                     /locus_tag="KKO15_000472"
     CDS             33846..34571
                     /gene="pyrH"
                     /locus_tag="KKO15_000472"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBD6359399.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34863..35420
                     /gene="frr"
                     /locus_tag="KKO15_000473"
     CDS             34863..35420
                     /gene="frr"
                     /locus_tag="KKO15_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBD6359400.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35512..36708
                     /gene="ispC"
                     /locus_tag="KKO15_000474"
     CDS             35512..36708
                     /gene="ispC"
                     /locus_tag="KKO15_000474"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBD6359401.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36897..37655
                     /gene="ispU"
                     /locus_tag="KKO15_000475"
                     /gene_synonym="uppS"
     CDS             36897..37655
                     /gene="ispU"
                     /locus_tag="KKO15_000475"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBD6359402.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37668..38525
                     /gene="cdsA"
                     /locus_tag="KKO15_000476"
     CDS             37668..38525
                     /gene="cdsA"
                     /locus_tag="KKO15_000476"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD6359403.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38537..39889
                     /gene="rseP"
                     /locus_tag="KKO15_000477"
     CDS             38537..39889
                     /gene="rseP"
                     /locus_tag="KKO15_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBD6359404.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39919..42351
                     /gene="bamA"
                     /locus_tag="KKO15_000478"
     CDS             39919..42351
                     /gene="bamA"
                     /locus_tag="KKO15_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBD6359405.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPLDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42473..42958
                     /gene="skp"
                     /locus_tag="KKO15_000479"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42473..42958
                     /gene="skp"
                     /locus_tag="KKO15_000479"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBD6359406.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42962..43987
                     /gene="lpxD"
                     /locus_tag="KKO15_000480"
     CDS             42962..43987
                     /gene="lpxD"
                     /locus_tag="KKO15_000480"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBD6359407.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQSNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44092..44547
                     /gene="fabZ"
                     /locus_tag="KKO15_000481"
     CDS             44092..44547
                     /gene="fabZ"
                     /locus_tag="KKO15_000481"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBD6359408.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44551..45339
                     /gene="lpxA"
                     /locus_tag="KKO15_000482"
     CDS             44551..45339
                     /gene="lpxA"
                     /locus_tag="KKO15_000482"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBD6359409.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45339..46487
                     /gene="lpxB"
                     /locus_tag="KKO15_000483"
     CDS             45339..46487
                     /gene="lpxB"
                     /locus_tag="KKO15_000483"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBD6359410.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46484..47080
                     /gene="rnhB"
                     /locus_tag="KKO15_000484"
     CDS             46484..47080
                     /gene="rnhB"
                     /locus_tag="KKO15_000484"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBD6359411.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47117..50599
                     /gene="dnaE"
                     /locus_tag="KKO15_000485"
     CDS             47117..50599
                     /gene="dnaE"
                     /locus_tag="KKO15_000485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBD6359412.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50612..51571
                     /gene="accA"
                     /locus_tag="KKO15_000486"
     CDS             50612..51571
                     /gene="accA"
                     /locus_tag="KKO15_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBD6359413.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51670..53811
                     /gene="ldcC"
                     /locus_tag="KKO15_000487"
     CDS             51670..53811
                     /gene="ldcC"
                     /locus_tag="KKO15_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD6359414.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAASSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53868..54257
                     /locus_tag="KKO15_000488"
     CDS             53868..54257
                     /locus_tag="KKO15_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6359415.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54322..55620
                     /gene="tilS"
                     /locus_tag="KKO15_000489"
     CDS             54322..55620
                     /gene="tilS"
                     /locus_tag="KKO15_000489"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBD6359416.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55669..55929)
                     /gene="rof"
                     /locus_tag="KKO15_000490"
     CDS             complement(55669..55929)
                     /gene="rof"
                     /locus_tag="KKO15_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBD6359417.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55916..56116)
                     /locus_tag="KKO15_000491"
     CDS             complement(55916..56116)
                     /locus_tag="KKO15_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBD6359418.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56282..56827
                     /locus_tag="KKO15_000492"
     CDS             56282..56827
                     /locus_tag="KKO15_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBD6359419.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56824..57246
                     /gene="arfB"
                     /locus_tag="KKO15_000493"
     CDS             56824..57246
                     /gene="arfB"
                     /locus_tag="KKO15_000493"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD6359420.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57260..57970
                     /gene="nlpE"
                     /locus_tag="KKO15_000494"
                     /gene_synonym="cutF"
     CDS             57260..57970
                     /gene="nlpE"
                     /locus_tag="KKO15_000494"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBD6359421.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58125..58949)
                     /locus_tag="KKO15_000495"
     CDS             complement(58125..58949)
                     /locus_tag="KKO15_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBD6359422.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59002..60720)
                     /gene="proS"
                     /locus_tag="KKO15_000496"
     CDS             complement(59002..60720)
                     /gene="proS"
                     /locus_tag="KKO15_000496"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBD6359423.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60831..61538)
                     /gene="tsaA"
                     /locus_tag="KKO15_000497"
     CDS             complement(60831..61538)
                     /gene="tsaA"
                     /locus_tag="KKO15_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBD6359424.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61535..61939)
                     /gene="rcsF"
                     /locus_tag="KKO15_000498"
     CDS             complement(61535..61939)
                     /gene="rcsF"
                     /locus_tag="KKO15_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBD6359425.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62057..62872)
                     /gene="metQ"
                     /locus_tag="KKO15_000499"
     CDS             complement(62057..62872)
                     /gene="metQ"
                     /locus_tag="KKO15_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBD6359426.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LIAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62912..63565)
                     /gene="metI"
                     /locus_tag="KKO15_000500"
     CDS             complement(62912..63565)
                     /gene="metI"
                     /locus_tag="KKO15_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBD6359427.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63558..64589)
                     /gene="metN"
                     /locus_tag="KKO15_000501"
     CDS             complement(63558..64589)
                     /gene="metN"
                     /locus_tag="KKO15_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBD6359428.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64777..65352
                     /gene="gmhB"
                     /locus_tag="KKO15_000502"
     CDS             64777..65352
                     /gene="gmhB"
                     /locus_tag="KKO15_000502"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBD6359429.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65716..>65976
                     /locus_tag="KKO15_000503"
     rRNA            65716..>65976
                     /locus_tag="KKO15_000503"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15608 a        16338 c        18190 g        15840 t
ORIGIN      
        1 aatgtttaaa ctaaaatttt tattaaggaa agagaggatg tatgattttc agaaaatatt
       61 tatattcgta atataaataa attaccattt gcttaatttg tcaaatgctc ttgtatgtaa
      121 gacttcacgc aacgtctccc catcaggaaa cgccagctcc ggtgcaatct caaacagcgg
      181 ccacagcata aatccacgat tcttcatatc gtagtgcgga acggtcaggc gttcggtatt
      241 tatcacttca ttaccaaaca gcatgatatc gagatccagc gtgcgtggtc cccagcgttc
      301 agctttacga acacgaccct gttgcagttc tatgcgctgt gtatggttga gtaactcttc
      361 cggggcgggc gaagtttcaa gcgccacggc ggcgttgagg taatcgggtt gatcttgcgg
      421 ccccagcggt ggggtgcggt aaaacgaaga aacggcaaga atgcggcttt cagggatatc
      481 gcctaatgct ttcagggcag cattgacctg ctccagcgga gaggccagat tgctgcctat
      541 ggcaatatac gccactgtca tgtggtaccc tcacgacgcg gtgcgcgttt gcgtggacga
      601 cgagtacgac gacgcggtga cggttcttca tccagctcgt taagcatccc tttttgatct
      661 ggtggtgcgg aaacctggaa ctcaccccac catttcacca gacgctgcag ttcagcatta
      721 cgttcaactt cagctcgcaa ggccaacagg tcataagccg cacggaactt aggatgctcc
      781 agcagtttcc atgcgcgttt accctgacga cgggacatac gcaactgcaa ctgccagata
      841 tcgcgggtta acgtcgtcag acgtttcggg attgccagtg aacggcaggc ttcgtccagc
      901 acgtcgttca tcgccagcgc gaaagcgtcg tgataggtca ggccgctttc ctgggcgatc
      961 ttctgtgccg tctccagcag tgggtaccag aacatggcgg caaacaagaa cgccgggttc
     1021 acgcgcatat cgttatggat acgcgtatcg gtattcttca gcacctgttc aatgatccgc
     1081 tccatcgggc tgtcgccatt ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg
     1141 aacagatgat attcacacaa cagcttatag gtttcgtaac cgtagcccgc ttgtagcagt
     1201 ttaagcgatt cttcaaacag gcgtgccggt gggatatcgt tcagcagggt agcgaggcga
     1261 gggatcggtt ctgcggtttc cgggctgatg cgcataccca atttggcggc aaaacgtacc
     1321 gcgcgcagca tacgtaccgg atcttcacgg tagcgcgttt ccgggttacc aatcagacgg
     1381 ataacgccgt ccttcagatc cttcatacca ccaacgtaat cacggacggt aaaatccgct
     1441 acgctgtaat acaggctgtt gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag
     1501 ccgaaaatgt tgtcgcgcag caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg
     1561 ctgacgttac cttcgtggtg tccacggaag gtcgcaactt cgataatctc cgggccaaac
     1621 attacatgag ccagacggaa acggcgaccc accaggcggc agttacggaa cagtttgcgc
     1681 acctgctcag gcgtggcgtt agtggttacg tcaaaatctt tcggcttttt gccaagtaac
     1741 aggtcgcgca cgccgccgcc aaccagccag gcttcgtatc ccgctttgtt gagcctgtac
     1801 attaccttca gggcattttc actgatatct ttgcgggaaa tagcatgctg ctcacgcggg
     1861 atcaccgtca cctgtggacg ggcgactgcc tgttcagcct cgctttcctc gcggcttagc
     1921 accttgcggc aaaaattagc gactcgggta aaaatagtac acctcggtag tgtcaaacat
     1981 cattcaggac aaaaaaatag cggctaatca tagctcagca tgacgcattt gagaatgttg
     2041 aatttacaat tgccgactcg ggcacggcgg tcagcatcca gtttttgacg gctgactgaa
     2101 ggatttgctc gacgctgaaa tcctgccagt gtgcttctgc ctgctgcccc agaaattgaa
     2161 gtgccgcgat tagtaccggg cgtggatcgc ctttcggcaa cgcaggcgca tgattctgct
     2221 tggaaagttt agcgccttgt ggattaagcg ccagcggcag atgaatgtaa tctggcactt
     2281 tccagccaaa aagctggtac agcgggattt gccttactgt tggttcaatc agatcagccc
     2341 cacgcactat ttctgtaacg ccctggaaat gatcatcaac cacaacagcc aggttgtagg
     2401 cgaacaaccc atcacggcga tgaatgataa aatcttcccg tgccagtttt tcgtcggcgt
     2461 gaataatgcc tcgcagcagg tcagtaaatt gcgtgaccgg atgctgctgg cggatacgca
     2521 ctgcggcgtt gtctggtcca tgatgcaaca cccggcaatg accgtcgtaa ataccgccaa
     2581 tgctttgaat acgcgcacgc gtacaggtgc agtaataact tagtccttgt tcatgtaacc
     2641 aggcgagtgc ttcacgatag gcgtcgtgac gttgcgattg ccagagaaca tcgccgtccc
     2701 agtgcagacc gtaatgttcc agctggcgca ggatagtttc tgcggcaccg ggaacttcac
     2761 gaggcgggtc gatatcttct atgcgtacca gccagcgacc ttgccgggcg cgagcctgca
     2821 aatagctgcc gagcgcggcg atcagagagc caaaatgaag ctcgccggaa ggagaggggg
     2881 cgaagcggcc aatatactgt gtgtctgtca tctctttgaa caaaaaataa ggcgggagca
     2941 tttcccgcct gtggtaaacg tgatggaacg gctgtaatta gccagccatc tgtttttcgc
     3001 gaatttcagc cagcgttttg cagtcgatgc acagatcggc tgtcgggcgc gcttccagac
     3061 ggcgaatacc aatttcaaca ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt
     3121 ttttcagcgt cttctcgatc tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt
     3181 cgaggctgaa ctcttcttcc tgggctgcac ggtctaccgg gtccgggaag ttggctgctt
     3241 catcctgcat atgtgtaacg gtgcgatcga cttcatccct gagttgatta cgccatgctt
     3301 ccagaatacg acggaagtgc gccagctggg cttcattcat atactcttcg cccggcttct
     3361 cctgatatgg ttccacccca gcgatggcga gaatactcag ggacgatgtt ttacggtttt
     3421 gcccttcttg catgttgctt ctccttaaca cgcactatcg atccccatgt tcgggggaaa
     3481 aatgaggccg ctataaatag cagatgcttt tccggatagc aattatctaa acgtaacact
     3541 tgacaactgt gtgaggaaaa gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt
     3601 acttacttta ctacaatgta accggcagtg attttttaag agccatgcct tcagcagaaa
     3661 tttccgcttt gtaagccaga atttctaccc ccctctgttg agcttctgac aatagttgcg
     3721 cgtatttctc atcgatgtgg cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg
     3781 cgaaaaaaat aaccgcacgc tggccttcag ccgctacgct catcaactcc cgaaggtgtt
     3841 tctgacctcg ttcagtgacc gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa
     3901 ccgatttcac ttcaatatag cagtctggac gcgaatccgc ctgcaacata aagtcaatac
     3961 ggctgcgttc ggagccgtat tttacttcgc ttttcagcga gctatagcct gacagttctg
     4021 aaattgattc attaaggata gcctctttcg tcaacctgtt agcccaaagc gtattgacgc
     4081 aaataaatgc gccgctctgg ctttgagtta attcccaggt gtgtgggtat ttccgtttgg
     4141 tgttgtctga agtcgaatac cagacggtat cgccaggcgt tgcacaaccg gtcatcgcac
     4201 ccgtattcgg gcagtgtagc gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa
     4261 aacgtttgta acgctgaatt agcgtcgcgc gctgtagagg gggagaaaat tccattgcga
     4321 caatccttgt tattgcgtta gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg
     4381 ggcaaacgag gaggcgtaaa gggtgaactc cgtcaccgca taagaccagt taaaacccgg
     4441 cggcgggaat gtcaccgcct cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg
     4501 acgattgctt tgaaaacaac cgctgcgggc agcctgtgaa cggagcatat tcgccagctg
     4561 gattaagccg cgtggaggct gacgcatccc taaccacacc acacgcgaac gcagccattg
     4621 tccggcgtca tcaagcgtga gtgtgaaacc aggttgacga atccgtccgg ctaaaagaga
     4681 aagcgccttc tctttctctg cgctcacttc gcctaaaaat gccagagtca ggtgcaaatt
     4741 atcggcggcg accggacgtc ccgcctcagg tgggaagtgt gtggcgcgcc aatggataat
     4801 ctgttcgcgg atttctgcag gtaagtcgat agcaaagaac agacgttgcg gttcagacat
     4861 gtgaggcact cggttataaa ttaccgcgat gctacaatgt ggcgcgaaga atgttaaccc
     4921 tctggagcgt tttgtgtcgt cgttgcccgt tgctgccgtc ttacctgaat tacttaccgc
     4981 tctcgattgt gcgccgcagg tattattaag tgcgccgacc ggggccggga aatcaacctg
     5041 gctgccgctg caactgctgg cgcatcccgg cattaacggg aaaattatcc tgctggagcc
     5101 gcgtcgtctg gcggcgcgta acgtcgcgca acggctggcg gagctgctta acgaaaagcc
     5161 aggcgatacc gttggctacc ggatgcgtgc gcaaaactgc gtcgggctga atacccgcct
     5221 ggaagtggtt accgaaggcg tgctgacgcg gatgatccag cgtgacccgg aactgagcgg
     5281 tgttggattg gtgatccttg atgagtttca tgagcgcagt ttgcaggcgg atttggcgtt
     5341 ggcgctgtta ctcgatgtgc aacaaggtct gcgtgatgac cttaaactgc tgattatgtc
     5401 ggctacgctg gacaatgacc gcttgcagca aatgctgccg gaagcgcccg tcgtcatctc
     5461 agaagggcgc tcgtttccgg ttgaacgccg ttatttaccg ctgcccgcgc atcagcgttt
     5521 tgacgaagcc gttgcggtag ccaccgccga aatggtgcgt caggaaagtg gatcattact
     5581 gttattttta cctggcgtcg gagaaattca gcgcgtgcag gaacaactgg cttcgcgcat
     5641 cggcagtgat gtggtgctct gcccgctgta tggcgtgttg tcactgaacg atcagcgaaa
     5701 agcgatcctc ccggcaccgc aagggatgcg caaagtggtg ctggcgacca atattgctga
     5761 aaccagttta accatcgaag gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc
     5821 gcgctttgat ccgcgcacgg ggcttacgcg gttgattact caacgcgtta gtcaggcatc
     5881 catgacgcag cgtgccgggc gcgccgggcg tctggagccg ggtatcagcc tgcatttaat
     5941 cgccaaagaa caagcagaac gcgccgcggc gcaaagtgag ccggagatct tacaaagcga
     6001 tctttccggt ttgctgatgg aattactgca atggggatgc agcgatccgg cgcagatgag
     6061 ctggctggat caaccgccaa cggtgaatct actggccgcg aaacgcctgt tacaaatgct
     6121 gggggcgctg gagggtgaac ggcttagtgc gcaagggcaa aaaatggcag cgctgggtaa
     6181 cgatccgcgt ttagcggcaa tgctggttaa cgcaaagagc gatgacgaag ctgctaccgc
     6241 ggcaaaaatt gccgccattc tcgaagagcc gccacggatg ggcaatagtg acctgggcgt
     6301 ggcgttttcg cgcaatcaac cagcctggca gcaacgtagt cagcaactgt taaaacgctt
     6361 aaacgtacgc ggcggtgagg cagacagttc gcttatcgcg ccgctacttg ccggggcgtt
     6421 tgccgatcgc attgctcatc gccgtgggca agatggacgc tatcaactgg caaacagcat
     6481 gggagcgatg ctcgatgccg acgacgcgct aagccgccac gaatggttga tcgcaccgtt
     6541 attattgcag ggcagcgcct cgccggatgc gcggatttta ctggcgctac cggtcgatat
     6601 tgatgaatta gtacaacgct gtccgcagct ggtgcagcaa tctgacaccg tggaatggga
     6661 tgacgcgcaa ggtacgctga aagcctggcg tcggctacaa atcggtcagt tgacggtgaa
     6721 agtgcagccg ctggcgaaac cgtcagaaga cgagttgcat caggcgatgc ttaatggcat
     6781 ccgtgataaa ggtttaagcg tgctcaactg gacggcggaa gcggaacagc tacgcttgcg
     6841 tttgttatgc gccgcaaagt ggttgccgga atatgactgg ccagcggttg atgatgaaag
     6901 tttgttggcg acgctggaaa cgtggctgct gccacatatg accggcgtac attcactacg
     6961 cggcctgaaa tcactcgaca tttatcaggc actacgcgga ttacttgatt ggggaatgca
     7021 gcaacgtctg gatagtgaat tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc
     7081 cattcgttat catgaagata acccgcccgc gctggcggtg agaatgcagg agatgtttgg
     7141 tgaggccact aatccgacga tcgcccaggg gcgcgtgccg ctggtgctga agttgctttc
     7201 ccctgcccaa aggccgctgc aaatcacgcg tgatttgggg gccttctgga aaggggcgta
     7261 tcgtgaggtg caaaaggaga tgaaagggcg ttatcccaaa cacgtctggc cggacgaccc
     7321 ggcaaacacg gcaccgacgc gacggacgaa aaagtattcg taaggttggt tttctccctc
     7381 tccctgtggg agagggccgg ggtgagggca tcagcgcgca cgttcaccct caccctaacc
     7441 ctctccctca agggagaggg gaccgatcga gcgcaaattt tgagagatat cttcttctgt
     7501 cttgtaacag aagaacagaa aatcgggctt ttgcgcctga atattgcgga gaaaaagcat
     7561 ggccgggaat gaccgcgagc caattggacg caaagggaaa ctgacgcgtc cggtcaaaca
     7621 aaaggtaagc cgtcgtcgtt acgaagatga cgatgattac gacgattatg atgactatga
     7681 ggatgaagaa ccgatgccgc gcaaaggtaa gggcaaaggc aaagggcgta agcctcgtgg
     7741 caaacgcggc tggctatggc tactgctaaa actggctatc gtttttgccg tgctgatcgc
     7801 catttacggc gtttatctcg atcaaaaaat tcgtagccgt attgatggca aggtctggca
     7861 actgcccgcg gcagtttatg gccgaatggt caaccttgaa ccagatatga ccatcagcaa
     7921 gaacgaaatg gtgaagctgc tggaggcaac acagtatcgt caggtgtcga aaatgacccg
     7981 tcctggcgaa tttaccgtgc aggccaacag cattgagatg atccgccgtc cgtttgattt
     8041 cccggacagt aaagaagggc aggtgcgtgc gcgtctgacc tttgatggtg atcatctggc
     8101 gaccatcgtc aatatggaga acaaccgtca gttcggtttc ttccgtcttg atccgcgtct
     8161 gatcaccatg atctcctcgc caaacggtga gcagcgtttg tttgtgccgc gcagtggttt
     8221 ccctgatttg ctggtggata ctttgctggc gacagaagac cgtcattttt acgagcatga
     8281 tggaatcagt ctctactcca ttgggcgtgc ggtgctggca aacctgaccg caggtcgcac
     8341 ggtgcagggt gcgagtacgc tgacgcaaca gctggtgaaa aacctgttcc tctccagcga
     8401 gcgttcttac tggcgtaaag cgaacgaagc ttacatggcg ctgatcatgg acgcgcgtta
     8461 cagcaaagac cgtattcttg agctgtatat gaacgaggtg tatctcggtc agagcggcga
     8521 caacgaaatc cgcggcttcc cgctggcaag cttgtattac tttggtcgcc cggtagaaga
     8581 gctaagcctc gaccagcagg cgctgttagt cggtatggtg aaaggggcgt ccatctacaa
     8641 cccgtggcgt aacccgaaac tggcgctgga gcgacgtaat ctggtgctgc gtctgctgca
     8701 acagcaacag attattgatc aagaactcta tgacatgttg agtgcccgtc cgctgggggt
     8761 tcagccgcgt ggtggggtga tctctcctca gccagccttt atgcagctgg tgcgtcagga
     8821 gttgcaggca aaactgggcg ataaggtaaa agatctctcc ggcgtgaaga tcttcactac
     8881 ctttgactcg gtggcccagg acgcggcaga aaaagccgcc gtggaaggca ttccggcact
     8941 gaagaaacag cgtaagttga gcgatcttga aactgcgatt gtggtcgtcg accgctttag
     9001 tggtgaagtt cgtgcgatgg tcggaggttc tgagccgcag tttgcgggct acaaccgtgc
     9061 gatgcaggcg cgtcgttcga ttggttccct tgcaaaacca gcgacttatc tgacggcttt
     9121 aagccagccg aaaatctatc gcctgaacac gtggattgcc gatgcgccaa tcgcgctgcg
     9181 tcagccgaat ggccaggtct ggtcaccgca gaacgatgac cgtcgttata gcgaaagcgg
     9241 cagagtgatg ctggtggatg cgttgacacg ttcgatgaac gtgccgacgg taaatctggg
     9301 gatggcgctg gggctgcctg cggttacgga gacctggatc aaactgggcg taccgaaaga
     9361 tcagttgcat ccggttccgg caatgctgct gggggcgttg aacttaacgc caatcgaagt
     9421 ggcgcaggca ttccagacca tcgccagcgg tggtaaccgt gcaccgcttt ctgcactgcg
     9481 ttcggtaatc gcggaagatg gtaaggtgct gtatcagagc ttcccgcagg cagaacgcgc
     9541 tgttccggcg caggcggcgt atctgacact atggaccatg cagcaggtgg tacaacgtgg
     9601 tacgggtcgt cagcttgggg cgaaataccc gaacctgcat ctggcaggga aaacagggac
     9661 taccaacaat aacgtagata cctggtttgc aggcattgac ggcagcacgg tgaccatcac
     9721 ctgggtaggc cgtgataaca accagccgac caaactgtat ggagccagcg gggcaatgtc
     9781 gatttatcag cgttatctgg ctaaccagac gccaacgccg ctgaatcttg ttccgccaga
     9841 agatattgca gatatgggcg tggactacga cggcaacttt gtttgcagcg gtggcatgcg
     9901 tgtcttgccg gtctggacca gcgatccgca atcgctgtgc cagcagagcg agatgcagca
     9961 gcagccgtca ggcaatccgt ttgatcagtc ttctcagccg cagcaacagc cgcaacagca
    10021 acctgctcag caagagcaga aagacagcga cggtgtagcc ggttggatca aggatatgtt
    10081 tggtagtaat taacatctaa gcgtgaaata ccggatggcg agttgccatc cggtaaaata
    10141 acatcccatc taagatatta accctttctt ttcatctggt tgtttattaa cccttcagga
    10201 acgctcagat tgcgtaccgc ttgcgaaccc gccagcgttt cgaatattat cttatcttta
    10261 taataatcat tctcgtttac gttattattc actttacatc agagatatac caatggcgcg
    10321 ttccaaaact gctcagccaa aacactcact gcgtaaaatc gcagttgtag tagccacagc
    10381 ggttagcggc atgtctgttt atgcacaggc agcggttgaa ccgaaagaag acactatcac
    10441 cgttaccgct gcacctgcgc cgcaagaaag cgcatggggg cctgctgcaa ctattgcggc
    10501 gcggcagtca gctaccggca ctaaaaccga tacgccgatt caaaaagtgc cacagtctat
    10561 ttctgttgtg accgccgaag agatggcgct gcatcagccg aagtcggtaa aagaagcgct
    10621 tagctacacg ccgggtgtct ctgttggtac gcgtggcgca tccaacacct atgaccacct
    10681 gatcattcgc ggttttgcgg cagaaggcca aagccaaaat aactatctga atggcctgaa
    10741 gttgcagggc aacttctata acgatgcggt cattgacccg tatatgctgg aacgcgctga
    10801 aattatgcgt ggcccggttt ccgtgcttta cggtaaaagc agtcctggcg gtctgttgaa
    10861 tatggtcagc aagcgtccga ccaccgaacc gctgaaagaa gttcagttta aagccggtac
    10921 tgacagcctg ttccagactg gttttgactt tagcgatgcg ttggatgatg acggtgttta
    10981 ctcttatcgc ctgaccggtc ttgcgcgttc tgccaatgcc cagcagaaag ggtcagaaga
    11041 gcagcgttat gctattgcac cggcgttcac ctggcgtccg gatgataaaa ccaatttcac
    11101 cttcctttct tacttccaga acgagccgga aaccggttat tacggctggt tgccgaaaga
    11161 gggaaccgtt gagccgctgc cgaacggtaa gcgtctgccg acagacttta atgaaggggc
    11221 gaagaacaac acctattctc gtaatgagaa gatggtcggc tacagcttcg atcacgaatt
    11281 taacgacacc tttactgtgc gtcagaacct gcgctttgct gaaaacaaaa cctcgcaaaa
    11341 cagcgtttat ggttacggcg tctgctccga tccggcgaat gcttacagca aacagtgtgc
    11401 ggcattagcg ccagcggata aaggccatta tctggcacgt aaatacgtcg ttgatgatga
    11461 gaagctgcaa aacttctccg ttgataccca gttgcagagc aagtttgcca ctggcgatat
    11521 cgaccacacc ctgctgaccg gtgtcgactt tatgcgtatg cgtaatgaca tcaacgcctg
    11581 gtttggttac gacgactctg tgccactgct caatctgtac aatccggtga atcccgattt
    11641 cgacttcaat gccaaagatc cggcaaactc cggcccttac cgcattctga ataaacagaa
    11701 acaaacgggc gtttatgttc aggatcaggc gcagtgggat aaagtgctgg tcaccctagg
    11761 cggtcgttat gactgggcag atcaagaatc tcttaaccgc gttgctggga cgaccgataa
    11821 acgtgatgac aaacagttta cctggcgtgg tggtgttaac tacctgtttg ataatggtgt
    11881 aacaccttac ttcagctata gcgaatcgtt tgaaccttct tcgcaagttg ggaaggatgg
    11941 taatattttc gcaccgtcta aaggtaagca gtatgaagtc ggcgtgaaat atgtaccgga
    12001 agatcgtccg attgtagtta ctggtgccgt gtataatctc actaaaacca acaacctgat
    12061 ggcggaccct gagggttcct tcttctcggt tgaaggtggc gagatccgcg cacgtggcgt
    12121 agaaatcgaa gcgaaagcgg cgctgtcggc gagtgttaac gtagtcggtt cttatactta
    12181 caccgatgcg gaatacacca ccgatactac ctataaaggc aatacgcctg cacaggtgcc
    12241 aaaacacatg gcttcgctgt gggctgacta taccttcttt gacggtccgc tttcaggtct
    12301 gacgctgggc accggtggtc gttatactgg ctccagctat ggtgatccgg ctaactcctt
    12361 taaagtggga agttatacgg tcgtggatgc gttagtgcgt tatgatctgg cgcgagtcgg
    12421 catggcgggc tccaacgtgg cgctgcatgt taacaacctg ttcgatcgtg aatacgtcgc
    12481 cagctgcttt aacacttatg gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc
    12541 aacctttcgt ttctaatttc tcttttgggg cacggatttc cgtgcccatt tcacaagttg
    12601 gctgttatgc aggaatacac gaatcattcc gataccactt ttgcactgcg taatatctcc
    12661 tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa
    12721 gtgaccggtc tgattggtca caacggttct ggtaaatcca ctctgctcaa aatgcttggt
    12781 cgtcatcagc ctccgtcaga aggggagatt cttcttgatg cccagccgct ggaaagctgg
    12841 agcagcaaag cgtttgcccg caaagtggct tatttgccgc agcagcttcc tccggcagaa
    12901 gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc cgtggcatgg cgcgctgggg
    12961 cgctttggtg cggcagatcg cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa
    13021 ccgctggcgc atcggctggt cgatagtctc tctggcggcg aacgtcagcg ggcgtggatc
    13081 gccatgctgg tggcgcagga tagccgttgt ctgttgctcg acgaaccgac ttcagcgctg
    13141 gatatcgccc accaggttga tgtgctggcg ctggtgcacc gtttaagtca ggaacgtggc
    13201 ctgacggtca ttgccgtgtt gcacgatatc aatatggcgg cacgctactg tgattatctg
    13261 gtcgccctgc gcagtggtga aatgattgct cagggaacgc ctgcggaaat tatgcgcggc
    13321 gaaaccctcg aaatgattta tggcatcccg atgggtattt tgccgcatcc ggcgggtgct
    13381 gcacctgtga gttttgttta ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt
    13441 taacggcgat ggcgctttct ccgttgttat ggcagatgaa taccgcccac gcggcggcta
    13501 ttgaccccaa tcgtattgtg gcgctggagt ggttgccggt ggaattactg ctggcgctcg
    13561 gcatcgtgcc ttacggcgtg gcggatacca tcaactatcg cctgtgggtc agcgaaccac
    13621 cattgccgga ctcagtgatt gacgtcggtt tgcgtacaga acctaacctt gaactgatga
    13681 ccgaaatgaa accgtcgttt atggtctggt cggcaggata tggcccttca tcagaaatgc
    13741 tggcacgtat tgcgccgggt cgcggattta acttcagtga cggcaaacat ccgttggcga
    13801 tggcgcgtaa atcgctgacg gaaatggcag atttacttaa cctgcaaagc gcagcggaaa
    13861 cgcatttagc gcaatacgaa gactttatcc gcagcatgaa accccgcttt gtgaagcgtg
    13921 gtgcacgccc gttactgctg acgacgctta tcgatccgcg ccatatgctg gtcttcggtc
    13981 caaacagctt gttccaggaa attctcgatg agtacggcat cccaaatgcc tggcaagggg
    14041 aaaccaactt ctggggcagt accgccgtca gtatcgatcg tctggcagcg tataaagacg
    14101 ttgatgtgct ctgttttgat cacgacaaca gcaaagacat ggatgcgcta atggcaaccc
    14161 cgctgtggca ggccatgccg tttgtccgta cagggcgctt tcagcgcgta cccgcagtct
    14221 ggttttatgg tgcaacgctt tcggcaatgc actttgtgcg tgttctggat aacgccatcg
    14281 gaggtaaagc gtgagtaaac gaattgcgct tttcccggcg ttattgctgg cgctgttagt
    14341 gattgtcgct acggcgctca cctggatgaa cttctcacag gcgctgccgc gcagtcagtg
    14401 ggcgcaggcc gcctggtcgc cggatattga cgtcatcgag cagatgattt ttcactacag
    14461 cttgttgccg cgtctggcga tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt
    14521 gctgtttcag caagtactgc gtaacccgct ggcggaaccg acgacgttgg gcgttgcaac
    14581 aggcgcgcaa ctggggatta ccgtcaccac gctctgggcg atccctagcg cgatggcgag
    14641 ccagtttgct gcgctggcag gggcttgcgt tgttggctta attgtctttg gcgtcgcgtg
    14701 ggggaaacgg ctgtcgccgg taacgctgat tttcgcgggg ttggtagtga gcctttattg
    14761 cggcgcaatc aatcagttac tggttatctt ccatcatgac cagttgcaaa gcatgtttct
    14821 gtggagcact ggaacgctga cgcaaaccga ctggggcggt gttgagcgtt tatggccgca
    14881 gttgctgggc ggtgtgatgc tgacgttgct gctacttcgt ccgttaaccc tgatggggct
    14941 tgatgatggc gtggcgcgca atctcgggct ggccttgtcg cttgcgcgtc tggcggcgct
    15001 gtcgctggcg attgtcatca gtgcgctgct ggtgaacgct gtggggatta tcggctttat
    15061 cgggttgttc gcgccgctgc tggcaaaaat gctgggggcg cggcgtctgc tgccacgact
    15121 gatgctggcg tcgttgattg gtgcgctgat cctctggctt tccgatcaaa tcatcctctg
    15181 gctgactcgc gtgtggatgg aagtgtccac cggttcggtc actgcgttga tcggtgcgcc
    15241 gctgctactg tggctgctgc cgcgtttacg cagcattagc gcgccggata tgaaggttaa
    15301 cgatcgtgtc gcgactgaac gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct
    15361 gttgttgatg gctgtggtgg tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg
    15421 ggcgagcggg gcgttgctcg aggatttaat gccctggcgc tggccgcgaa ttatggcggc
    15481 gctgtttgcg ggcgtcatgc tggcggtagc gggctgtatt attcagcgac tgaccggcaa
    15541 cccgatggca agcccggaag tgctggggat tagctccggc gcggcgtttg gtgtggtgtt
    15601 aatgctgttt ctggtaccgg gtaatgcctt tggctggttg ttacccgcag gcagtctcgg
    15661 ggcggcggtg acgctgttga tcattatgat cgccgccgga cgcggtggat tttccccaca
    15721 ccgcatgtta ctggcgggga tggcgttaag taccgcgttc accatgcttt tgatgatgtt
    15781 gcaggcaagt ggtgacccgc gaatggcgca agtgctgacc tggatttccg gttcgaccta
    15841 caacgcgacc gatgctcagg tctggcgcac cggaattgtg atggtgattt tgctggcgat
    15901 tactccgctg tgccgccgct ggctgaccat tttaccgctg ggtggtgata ccgcgcgggc
    15961 ggtagggatg gcgctgacgc cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac
    16021 ggcgaccgcg acgatgacta ttggaccgtt gagttttgtt ggtttaatgg caccgcatat
    16081 tgcgcggatg atgggctttc gacggacgat gccacacatc gtaatttcgg cgctggtggg
    16141 tggtttactg ctggtgttcg ctgactggtg tgggcggatg gtgctgtttc cattccagat
    16201 cccggcgggg ctgctgtcga cctttatcgg tgcgccatat tttatctatt tgctgagaaa
    16261 gcagagccgt taatagaatg cctcgcccgg tgggcgaggc aaaattatca caacttcgcg
    16321 aacacccgac gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacagac
    16381 ataaagcccg cttcaaacgc tgacggtgcc aggtaaacac cttcatccag catcatatgg
    16441 aagaaacgct taaaacgctc gacgtcacag gccatcacat cctgatagca cgtcacggac
    16501 tcggcgtcgg taaagaagat accgaacata ccaccgacgt ggttaacgac cagcggaatt
    16561 cccgcttctt ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccagc
    16621 gtttcgtgaa tgcccggctg cgcgacttca ttcagacagg cgaaacccgc tgccatcgca
    16681 atcgggttac cggaaagcgt acccgcctga tagaccggac ctgtcggggc cagcgcatcc
    16741 attacatcac gacgaccacc gaatgcgcct accggcattc caccgccgat gatttttccg
    16801 aggcaggtta aatctggcac tacggcgaaa taatcctgtg cgccagctag cgctacgcgg
    16861 aaaccggtca tcacttcatc gatgatcagc aacgcgccaa attcgtcgca cagcgcacgc
    16921 agacctggca ggaactctgg cagcggcgga acacagttca tattgcctgc caccggctcg
    16981 acgataatac aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaggccaga
    17041 tcgttataag tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt
    17101 ggctggccta acgtgagtgc gccagaaccg gctttcacca gcaggcagtc agcgtgaccg
    17161 tggtaacaac cttcaaattt aatgattttg tcgcgaccgg taaaaccacg ggccaggcgg
    17221 atggcgctca tcgtcgcctc ggtgccggag ttcaccatgc gcaccatatc catggtcggg
    17281 accagttcgg tcaccagttg cgccattttc acttccattt cggtcggtgc accaaagctt
    17341 aaaccacgct cggcggcttc aatcacggca ttgcggatcg ccggatggtt atggcccagc
    17401 accatcggcc cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga
    17461 taagcgccgt ccgctttttc gataaacagt ggagtgccgc ccacgccagt aaaggcgcga
    17521 acaggggagt tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac
    17581 ttactcatgg agggttcctg attcgtagaa aaagtgaatg gctgctattc catgttattc
    17641 ataacaagtt aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct
    17701 tagagtataa aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac
    17761 gtgcgagtaa aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac
    17821 tgatactccc tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg
    17881 ccaacttctt gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg
    17941 cacgcttgtc gggctggctg cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca
    18001 acgtatgggg gcgctggtac atactgctga taattatccg ctactgttaa ccgtcgcttt
    18061 tctctgttcg gcggtgctgg cgatgttcgg ctactttttg gtgcgcaaat acgcgcccga
    18121 agcaggggga tcggggatcc cggaaattga aggggcgctg gaagatcaac gtcctgttcg
    18181 ctggtggcgt gtattgccgg tgaaattctt tggcgggctg gggacactcg gcggaggcat
    18241 ggggctaggg cgcgaaggac caaccgtgca gatcggcggc aatatcagtc gcatggtgct
    18301 tgatattttc cgcctgaaag gtgatgaagc tcgccatacg ctgctggcaa ccggtgctgc
    18361 tgcggggctg gctgcggcct ttaacgcgcc gctggcgggt attttgttta ttatcgaaga
    18421 gatgcgtccg cagtttcgct atacgttaat ttcgattaaa gcggtattta ttggcgtcat
    18481 tatgtcgacc attatgtacc ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa
    18541 actttctgat gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg
    18601 cattttcggc cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt
    18661 gcacggcggc aatattacca aatgggtact gatggggggt gcgattggcg gtctgtgtgg
    18721 attgctgggg tttgtggcac cagcaacgtc gggcggcggt tttaacctga ttcctatcgc
    18781 taccgcgggg aatttcagca tgggaatgct ggtgtttatc ttcgtcgctc gggtcattac
    18841 caccttactc tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct
    18901 gggtactgtg ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca
    18961 cctggaggcg gggacgtttg ctattgccgg aatgggggca ttactggcgg catctattcg
    19021 cgcgccgtta acggggatca ttctggttct ggagatgacc gataactacc agctcatttt
    19081 gccaatgatt attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc
    19141 tctatactcg gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg
    19201 aagcaaggcc gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg
    19261 gcgattatta ttgggttaga atttgcccga ttgccgatgt cgtttggagc aaaatatgag
    19321 tgatgacgta gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct
    19381 gatcgctgac gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg
    19441 cagcggcttc cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat
    19501 cgaaaaacag ggcgttggcc tggtggttga tccgatgagc ctgcaatatc tggtcggcgg
    19561 ttccgttgac tacaccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc
    19621 gaaaagcacc tgcggttgcg gttcttcctt tagcatctaa tctgttgttc atgtgccgga
    19681 taaactatcc ggcacatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt
    19741 agcttaagat gccaacgaat agccgccagc cgaatcaata gcgtcacgac catgcccatc
    19801 atactggctg tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg
    19861 ccgataatac aggcagttgc gtagatttct gtacgtaaaa tcatggggat ttcgcggacc
    19921 agtacatcac gaatgatccc gccgccaacg ccagtaatga cgcccataca aaccgcgatt
    19981 aacggaccgg cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga
    20041 ccaacggcgt ccagcaccgg caacatccat tttggtaagc gtcttggctg gcgcaccagc
    20101 acgatggtca gcatgctggt gaccatggca acgaccagat cggtgggatc tttcacccaa
    20161 aataccgggc cgtgatccag cgccatgtcg cgaattgttc cgtcgcctac tgcggtaacc
    20221 acgcccagta ccagaacacc aaaagggtcc atacgcaatt ttccggctaa caaaacgccg
    20281 tagatggcaa ataccgctgt gccgactata tccagccaat agacgagcat tgttcaatcc
    20341 ccactgagca cctgaaaagg tcaggcacta atctacctgt gaaagcgcat tacagagctg
    20401 ttgtgctgcg aggataatac gtgggcttgc acgttcaaac cagtcactcg tgagaggaat
    20461 aacgggaatt ttgagctgtt caccccagta ttgtttgatt ttaggaattt ggtccggtcc
    20521 gcctgtaatg acaatagcct gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg
    20581 ccagggaacc cggctgtctt taaagatgtt ttctccgcca caaacttcga gtacctggtt
    20641 ctgaatcgac tcttttccac tggtaaatgg cggattaatg ccgaattgca gaaaaacacg
    20701 ttttttaggt ttatcagcat attgcgcttt caattgcacg tactgatcca gcagggattg
    20761 cgcggcttgt tcggccttgt ctggttgcgg actccagggg gccagttgac gtaacgcatt
    20821 ggcaatttgt tcaatgcttg tcgcatcgac ccacatcact tttattccca gcgaagccag
    20881 ctggtcaacc tgccgctcgg cattacctcc acgccaggca atcaccagat cgggtttcag
    20941 cgcgacaatg cgttccagat tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc
    21001 ttgtggagga tagtcggaat agctgctgac cccaaccggc gtgatcccgg cggcaaaggc
    21061 aagttcagtg ttggcgggag aaagcgtgat gacgcgcggc gcggcgttga gccacagtgg
    21121 cgcgagaaaa gacagggcga ccagcgccct gaacagtgat ttagccatgt gccagtttct
    21181 gcaccagtga ctcaaccatc aggctggact gtttagcggc aacagccagg aactcatcga
    21241 agctaagatg agactgttga tcggccacgt cggagatggc gcgtacgaca acaaacggga
    21301 cgttgaaatt gtggcagaca tgggcgattg ccgtcgcttc catctctaca gcaatggcct
    21361 gtgggaagtt gtggcggatt ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc
    21421 taacaatcag gccacgtaca gcgttaagat tcagttcggc aatgcaggcc tcagcggcag
    21481 cgatcagttt atcgtcagct ttaaagcctg ccggacagcc tggtaactga ccgtattcat
    21541 aaccaaatgc cgtgacatcc gcgtcgtgat aacgcgcttc gtccgagaca acgatatcgc
    21601 ccactttcag cgttggtgcc aggccaccgg cagaaccggt gttaataatc acatctggct
    21661 tgcagtgttc caacagcaga gtggcaccca gcgccgcagc gactttaccg atgcccgatt
    21721 tcagaagcgc aacctcggtt ccattcagtt ggccggtata gatttcgcaa ccgccgagac
    21781 tgatagtttg acggttttcg attttgtcac gcagcagcgt aacttcttct tccattgcac
    21841 caatgatgcc gattttcata gatttactcg cgataagccc gatttgaagg catagtttac
    21901 catgcgctta cggggaagcg tatttctcac gcgggagagg acatggcaca gattgatttc
    21961 cgaaaaaaaa taaactggca tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat
    22021 gagatcctgc ggatcttcga gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt
    22081 cgtctgcaac aaaagaccca ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt
    22141 cttacccact cgatggaagt ccagcaggtg ggacgctaca tcgccaaaga aattttaagc
    22201 cgtctgaaag agcttaaatt gctggaagca tacggcctgg atgaactgac cggccccttt
    22261 gaaagcattg ttgagatgtc atgcctgatg cacgatatcg gcaatccgcc gtttggtcat
    22321 tttggcgaag cagcgataaa tgactggttt cgccaacgtt tgcacccgga agatgccgaa
    22381 agccagcctc tgactgacga tcgctgcagc gtggcggcac tacgtttacg ggacggggaa
    22441 gaaccgctta acgagctgcg gcgcaagatt cgtcaggact tatgtcattt tgaggggaat
    22501 gcacaaggca ttcgtctggt gcatacattg atgcggatga atctcacttg ggcacaggtt
    22561 ggcggtattt taaaatatac ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat
    22621 cactatttaa tgaaaaagcc gggttattat ctttctgaag aagcctatat tgcccgcttg
    22681 cgtaaagaac ttaatttggc gctttacagt cgttttccat taacgtggat tatggaagct
    22741 gccgacgaca tctcctattg tgtggcagac cttgaagatg cggtagagaa aagaatattt
    22801 accgttgagc agctttatca tcatttgcac gaagcgtggg gccagcatga gaaaggttcg
    22861 ctcttttcgc tggtggttga aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc
    22921 agtacggaag atcagttttt tatgtattta cgggtaaaca ccctaaataa actggtaccc
    22981 tacgcggcac aacgatttat tgataatctg cctgcgattt tcgccggaac gtttaatcat
    23041 gcattattgg aagatgccag cgaatgcagc gatcttctta agctatataa aaatgtcgct
    23101 gtaaaacatg tgtttagcca tccggatgtc gagcagcttg aattgcaggg ctatcgggtc
    23161 attagcggat tattagagat ttatcgtcct ttattaagcc tgtcgttatc agactttact
    23221 gaactggtag aaaaagaacg ggtgaaacgt ttccctattg agtcgcgctt attccacaaa
    23281 ctctcgacgc gccatcggct ggcctatgtc gaggctgtca gtaaattacc gtcagattct
    23341 cctgagtttc cgctatggga atattattac cgttgccgcc tgctgcagga ttatatcagc
    23401 ggtatgaccg acctctatgc gtgggatgaa taccgacgtc tgatggccgt agaacaataa
    23461 ccaggctttt gtaaagacga acaataaatt tttacctttt gcagaaactt tagttcggaa
    23521 cttcgggcta taaaacgaat ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga
    23581 ctgaaataca tgaaaaaaac cacattagca ctgagtgcac tggctctgag tttaggtttg
    23641 gcgttatctc cgctctctgc aacggcggct gagacttctt cagcaacgac agcccagcag
    23701 atgccaagcc ttgcaccgat gctcgaaaag gtgatgcctt cagtggtcag cattaacgta
    23761 gaaggtagca caaccgttaa tacgccgcgt atgccgcgta atttccagca gttcttcggt
    23821 gatgattctc cgttctgcca ggaaggttct ccgttccaga gctctccgtt ctgccagggg
    23881 ggccagggcg gtaatggcgg cggccagcaa cagaaattca tggcgctggg ttccggcgtc
    23941 atcattgatg ccgataaagg ctatgtcgtc accaacaacc acgttgttga taacgcgacg
    24001 gtgattaaag tccagctgag cgatggtcgt aagttcgacg cgaagatggt tggcaaagat
    24061 ccgcgctctg atatcgcgct gatccaaatc cagaacccga aaaaactgac cgcaattaag
    24121 atggcggatt ctgatgcact gcgcgtgggt gattacaccg tagcgattgg taacccgttt
    24181 ggtctgggcg agacggtaac ttccgggatt gtctctgcgc tggggcgtag cggcctgaat
    24241 gctgaaaact acgaaaactt catccagacc gatgcagcga tcaaccgtgg taactccggt
    24301 ggtgcgctgg ttaacctgaa cggcgaactg atcggtatca acaccgcgat cctcgcaccg
    24361 gacggcggca acatcggtat cggttttgct atcccgagta acatggtgaa aaacctgacc
    24421 tcgcagatgg tggaatacgg ccaggtgaaa cgcggtgagc tgggtattat ggggactgag
    24481 ctgaattccg aactggcgaa agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc
    24541 caggttctgc ctaattcctc cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc
    24601 tcactgaacg gtaagccgat cagcagcttt gccgcactgc gtgctcaggt gggtactatg
    24661 ccggtgggca gcaaactgac cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac
    24721 ctggaactgc agcagagcag ccagaatcag gttgattcca gctccatctt caacggcatt
    24781 gaaggcgctg agatgagcaa caaaggcaaa gatcagggcg tggtagtgaa caacgtgaag
    24841 acgggcactc cggctgcgca gatcggcctg aagaaaggtg atgtgattat tggtgcgaac
    24901 cagcaggcag tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg
    24961 ctggcactga acattcagcg cggcgacagc accatctatc tgttaatgca gtaatctccc
    25021 tcaacccctt cctgaatacg ggaaggggtt ctccttacaa tctgtgaact tcgccacaac
    25081 tccatacatc ttcatcatcc tttgggcatt tgcacaatgc cgtacgttac gtacttcctt
    25141 atgttaagcc gtgcataacg gaggacttat ggctggctgg catcttgata ccaaaatggc
    25201 gcaggatatc gtggcacgta ccatgcgcat catcgatacc aatatcaacg taatggatgc
    25261 ccgcgggcga attatcggca gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc
    25321 attgctggta ctttcacagg gacgagtcgt cgatatcgat gacgcggtag cacgtcatct
    25381 gcacggtgtg cggcagggga ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt
    25441 aattggcctg acaggtgaac cagagaatct gcgtaaatat ggcgaactgg tctgcatgac
    25501 ggctgaaatg atgctggaac agtcgcggtt gatgcacttg ttggcgcagg atagccgttt
    25561 gcgggaagaa ctggtgatga acctgattca ggcagaggag aatactcccg cacttctgaa
    25621 tgggcgcaac ggctggggat cgatctcaat caaccgcgag tggtggctat tgttgaggtc
    25681 gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact gcaaaacgcg
    25741 ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctaaccga aatggtggtg
    25801 ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg taagcgagtt
    25861 gaacaactga ttacccgcat gaaagagtac ggccagctgc gttttcgcgt ttcactgggc
    25921 aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa aacgacgatg
    25981 gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga tctgatgtta
    26041 cctgtgttac tcgacagttt gcgtggcgac tggcaggcca acgaactggc gcgaccgctg
    26101 gcgcggctga aagcgatgga caataacggc ttgctgcgac gaacgctggc ggcgtggttt
    26161 cgccacaatg tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg taataccctg
    26221 gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggcaa ttttgatgac
    26281 aggttgctgc tgtatgtggc gttacaactg gatgaagagc ggtagattat gcgttaaggt
    26341 tgaggcggcg ctggctcatt cccctcagcc cgaaggggag aggggaatgc ccgagttaac
    26401 cgtcaattcg ggattattta cgcgttagtt tttccagatc ggcttcaatc tcgctgatct
    26461 tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa
    26521 cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac cgtagattcg
    26581 ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg accgttttac
    26641 gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga
    26701 aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga tcaatttctt
    26761 caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct caaaatgagt
    26821 cagtaaattg tgcttatttt agcatttggc ttggcccgcc cggctcgact gtgatcgcag
    26881 aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta cctgctttat
    26941 tgtggcgata ttccatggga tggattagca catttatctt cgttttgcga attacatgac
    27001 agtccgaaac aagatgaaaa agtatttaaa aaagggggag gaagaatgag taggacgaat
    27061 tattctgagc cagaaagcat acgggcagca tcatgctgcc cgtatatgta ctcttaatcg
    27121 atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc aactttctta
    27181 acgataaccg cacagtagag gctgtatttg ccatcttttg acggcagatt acctgaaaca
    27241 accacagacc ccgccggaac gcgaccgtag tggatttcgc cggtttcacg gtcgtaaata
    27301 cgggtgctct gaccaatgta tacgcccata gagatgacgg aaccttcttc gacaatcacc
    27361 ccttcaacca cttcagagcg tgcgccaatg aagcagttat cttcaataat ggtcgggtta
    27421 gcctgcagcg gctccagcac gccgccgatg cccacgccac cggaaaggtg gacgttttta
    27481 ccaatctgcg cacaagaacc gacggtcgcc caggtatcaa ccatggtgcc ttcatcaaca
    27541 tatgcgccga tgttaacgta agacggcatc agcacggtgt tacgggcaat aaacgcaccc
    27601 tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt tctggaaacg tgcttcgtcg
    27661 tagtcggcga atttcatcgg cactttgtcg aagtagcggc tttctgcccc ttcgatcacc
    27721 tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg atgcgtcacc
    27781 cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag ggcgatcacc
    27841 tgatttaccg cttcgcgggt aacggtgtct gcattggctg gcgtgatctc ggcacggcgt
    27901 tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt ttcatatcag
    27961 taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg atgcacttcc
    28021 tgctgcaact cgttattaag cgcacgccgg tcagcggtgg caattatgaa taaatcttct
    28081 actcgctcgc caatggttgt aattcgggca ccatgaagcg aaattcccag atcggcaaaa
    28141 attttcccga ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc gaggaacgat
    28201 ttgcggtcgg tatgggtcgg caaaaacgtt acttcggttt caacagtaaa atggcgtaat
    28261 ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac tttgcgtcag cacctgctcc
    28321 agaccaaacc gaataacctc atggcgatct gcggacagcg ggctgccatc gggttccagc
    28381 acgataaagg tatccatcgc cataccgtcg cgagtggtga aaatttgtgc gtcgtgaaca
    28441 cttaaattgc ggcggtctaa ttcggcacag acggcggcaa acagataggg gcggtccggg
    28501 ctccagataa aaatctcggt gcctccacgc gtagcctgcg ggctaagcaa taccagcggt
    28561 ttgcttaaat catgctgtaa taaatggcgg gcatgccagg ccagttgatt tgggctatgg
    28621 cggacaaaat agttagcacg acagcgtgac caaatttggt gcagcgcctc ttcgtcgatg
    28681 ttatccatgc gcagtagtgc cagtgcctgg agttggtgat ggcgaacccg ttcgcgcata
    28741 tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcaaaata gagttcgcgc
    28801 aacagacttt gcttccagct attccacagt gtttcgttgg tggcgcaaat gtcagccact
    28861 gtcaggcata ccagatagcg cagacgattt tccgtttgaa cttcttcggc aaactgcttg
    28921 atgacttcag ggtcctgaat atcgcggcgt tgtgctgtca ccgacatcag caagtgctgg
    28981 tgaaccagcc aggcaaccag ctgtgtttcg cgtgagttca gcccgtggag ttctgcaaaa
    29041 tgcactacat cctgagcacc gagaatggag tggtcgccgc cgcgtccttt ggcgatatcg
    29101 tgaaacagcg cggcgatgaa aatcagctca gttgacggca ggcgcggcca gacgtccaca
    29161 cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt cagcatcacg
    29221 cggatagtat gttcatccac cgtgtaggcg tggaacagat caaactgcat ctgcccgacg
    29281 atatgcgacc attgcggcat atacgcgccg agcacgctat ggcgatgcat tggcaatagt
    29341 ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa acagttttcg tgcttgcgga
    29401 atattacaca gcggttgttg cagatggcga cgggcatggc gtaactggcg cagcgtggtg
    29461 gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg caagatggct
    29521 tccggctggc gcataaatag tgtttcatca cgcaggtcga ttagcgtacc gcgtagctga
    29581 aactcatcgt cgagtggacg tggtttttcg tcggcgggaa gggcgaggat ggcttcatcg
    29641 aacagttgca gcagcatctg gttgagttca ctgacacggc gtgtaacgcg gaagtaatcc
    29701 ttcatcatcc gctcgaccgg ttcgttgcct tcaccactgt aattcagacg ctgagcgacg
    29761 ctaagttggc gatcgaataa cagacgatta tcgtaacggc tgacgaccag atgcagggca
    29821 aagcgaatac gccacagtat atgcagacat tcgtttaatt ccgcccgctc cgctgaggtt
    29881 aagaagccaa acccgaccat ttcatccagc gatgttgcgc caaaatgacg gcgggccacc
    29941 cattgcagag tgtgaatatc gcgcaagccg ccagggctac ttttgatgtc tggttcaagg
    30001 ttgtagctgg tgccatggta acgctgatgg cgctggttct gttcttcaac tttcgctgcg
    30061 tagaacttgt cggaaggcca gaatccttcg ctaaaaatat gtttttgcag ttcgaggaag
    30121 agcgcaacat cgccaattaa taagcgggat tcgattaaat tggtggcgac ggttaaatcc
    30181 gataaccctt ccaacatgca ctcttcaagc gtgcgcacac tatgaccgac ttccagcttt
    30241 acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcgtc cgggagcttt
    30301 ttacggctta aaatcagtaa atcgatgtca gaaagtggat gcagctcgcc acgaccgtag
    30361 ccaccgacgg caaccaatgc caggtcggca atctggctga atcccgcttc aatccataat
    30421 cgttgcagga gctggtctat gaactcggta cgtgcctcaa tcaactgttc tgcagagatc
    30481 ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc ttttatccca
    30541 cagacggtta attcatcacg gggccaggcg catggatttt gcggttgacc gggcagggtg
    30601 gagagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca cccataagat
    30661 taaattatcg cattaaaaaa gccggcattc gccggcttca tcttattcgt cgtgcgagat
    30721 tatcgccggg atggtgtcat ccttgcgtag cgtcaaaatt tcgcagccgt tatcagtcac
    30781 cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta ccgtccagcc
    30841 atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcaa tggtgaacgt
    30901 catccccggt ttcagcacga cgttggtttc acgggagtca tagtgcagca cctgcggttc
    30961 ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca cgaacgacgg agaagccttc
    31021 tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc agattaatgc ctggttttac
    31081 catgcgtagc gccaggtaca ggctttcttg cgtgatgcgg cacaggcgtt cgcccatgat
    31141 ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat ctttgattac
    31201 ggtgacatca atgttaacga tatcgccatc tttcagcagc ttagcatcgt ccgggatacc
    31261 gtggcacacc acttcattaa tagagatgca aacggatttc ggatagccgt gatagccgag
    31321 gcaggcagaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc gatccagctc
    31381 gccggtgctg acgcccggtt taacatacgg ttcgatcatc tccagcactt cggcagccag
    31441 tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga tagccattaa
    31501 ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc aatggtatca
    31561 cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga aggttttcta
    31621 taatagaaaa ttcgacgtct gatgctgtac acagcgccaa caattattgg tgtccacgac
    31681 gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca gactggacgg
    31741 aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc cggggtgccc
    31801 tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata tagaggtttt
    31861 aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact tcggtcacca
    31921 gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta acaaagttca
    31981 catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg aactgaacaa
    32041 gattgtttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg caagcgaagc
    32101 ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct ggctgggcgg
    32161 tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag acctggaaac
    32221 tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga tgcgcactcg
    32281 tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg gtctgccgga
    32341 cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagaag caaacaactt
    32401 gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg ttgacttcgt
    32461 tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg ctgttgctgc
    32521 aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa gcttcgtaga
    32581 agctgagtaa taaggcttga taactccccc aaaatagttc gagttgcaga aaggcggcaa
    32641 gctcgagaat tcccgggagc ttacatcagt aagtgaccgg gatgagcgag cgaagacaac
    32701 gcatctgcgg cgcgaaatat gaagggggag agcccttata gaccaggtag tacacgtttg
    32761 gttagggggc ctgcatatgg cccccttttt cacttttata tctgtgcggt ttaatgccgg
    32821 gcagatcaca tctccgagga ttttagaatg gctgaaatta ccgcatccct ggtaaaagag
    32881 ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac tgaagctaac
    32941 ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa agcagcgaaa
    33001 aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg caactacggc
    33061 atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg tttccaggcg
    33121 ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt tgaagttctg
    33181 aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga aaacatcaac
    33241 attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca cggtgcgcgt
    33301 atcggcgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca catcgctatg
    33361 cacgttgctg caagcaagcc agaattcatc aaaccagaag acgtatccgc tgaagtggta
    33421 gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc gaaagaaatc
    33481 gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc tctgaccggt
    33541 cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga gcataacgct
    33601 gaagtgactg gcttcatccg tttcgaagtg ggtgaaggca tcgagaaagt tgagactgac
    33661 tttgcagcag aagttgctgc gatgtccaag cagtcttaat tatcaaaaag gagccgcctg
    33721 agggcggctt ctttttgtgc ccatcttgta aattcagcta acccttgtgg ggctgcgctg
    33781 aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag tctcaggaaa
    33841 gaaacatggc taccaatgca aaacccgtct ataaacgcat tctgcttaag ttgagtggcg
    33901 aagctctgca gggcactgaa ggcttcggta ttgatgcaag catactggat cgtatggctc
    33961 aggaaatcaa agaactggtt gaactgggta ttcaggttgg tgtggtgatt ggtgggggta
    34021 acctgttccg tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg ggcgaccaca
    34081 tggggatgct ggcgaccgta atgaacggct tggcaatgcg tgatgcactg caccgcgcct
    34141 atgtgaacgc tcgtctgatg tccgctattc cattgaatgg cgtgtgcgat agctacagct
    34201 gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc gccggtacag
    34261 gtaacccgtt ctttaccacc gactcagcag cttgcctgcg tggtatcgaa attgaagccg
    34321 atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac cgctgatccg gcgaaagatc
    34381 caaccgcaac catgtacgag caactgactt acagcgaagt gctggaaaaa gagctgaaag
    34441 tcatggacct ggcggccttc acgctggctc gtgaccataa attaccgatt cgtgttttca
    34501 atatgaacaa accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa gggactttaa
    34561 tcacggaata attcccgtga tggataaata agggtaagat tccgcgtaag tatcgcgggg
    34621 gcgtaagtct ggttataagg cgttattgtt gcaggcagtt tggtcacggc cagcgcgcag
    34681 caaccggagc gtacaaaagt acgtgaggat ggcgagcact gctcggggcc aaaatggcaa
    34741 ataaaatagc ctaataatcc agacgattac ccgtaatatg tttaatcagg gctatactta
    34801 gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca aggattcgta
    34861 acgtgattag cgatatcaga aaagatgctg aagtacgcat ggacaaatgc gtagaagcgt
    34921 tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg ctggatggca
    34981 ttgtcgtgga atattacggc acgccgacgc cgctgcgtca gctggcaagc gtaacggtag
    35041 aagattcccg tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg gccgttgaaa
    35101 aagcgattat ggcctccgat ctcggcctga acccgaactc tgcgggtagc gacatccgtg
    35161 ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc gttcgtggtg
    35221 aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac gacaaagtga
    35281 aagcactgtt gaaagataaa gagatcagcg aagacgacga tcgccgttct caggacgatg
    35341 tacagaaact gactgatgct gcaatcaaga aaattgaagc ggcgctggca gacaaagaag
    35401 cagaactgat gcagttctga tttcttgaac gacaaaaacg ccgctcagta gatccttgcg
    35461 gatcggctgg cggcgttttg ctttttattc tgtctcaact ctggatgttt catgaagcaa
    35521 ctcaccattc tgggctcgac cggctcgatt ggttgcagca cgctggacgt ggtgcgccat
    35581 aatcccgaac acttccgcgt agttgcgctg gtggcaggca aaaatgtcac tcgcatggta
    35641 gaacagtgcc tggaattctc tccccgctat gccgtaatgg acgatgaagc gagtgcgaaa
    35701 cttcttaaaa cgatgctaca gcaacagggg agccgcaccg aagtcttaag tgggcaacaa
    35761 gccgcttgcg atatggcagc gcttgaggag gttgatcagg tgatggcagc cattgttggt
    35821 gctgctgggc tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat tttgctggcc
    35881 aataaagaat cactggttac ctgcggacgt ctgtttatgg acgccgtaaa gcagagcaaa
    35941 gcgcaattgt taccggtcga tagcgaacat aacgctattt ttcagagttt accgcaacct
    36001 atccagcata atctgggata cgctgacctt gagcaaaatg gcgtggtgtc cattttactt
    36061 accgggtctg gtggcccttt ccgtgagacg ccattgcgcg atttggcaac aatgacgccc
    36121 gatcaagctt gccgtcatcc gaactggtcg atgggacgta aaatttctgt cgattcggct
    36181 accatgatga acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa cgccagcgcc
    36241 agccagatgg aagtgctgat tcacccgcag tcagtgattc actcaatggt gcgctatcag
    36301 gacggcagtg ttctggcgca gctgggggaa ccggatatgc gtacgccaat tgcccacacc
    36361 atggcatggc cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg caaactaagt
    36421 gcgttgacat ttgccgcacc ggattatgag cgttatccat gcctgaaact ggcgatggag
    36481 gcgttcgaac aaggccaggc agcgacgaca gcattgaatg ccgcaaacga aatcaccgtt
    36541 gctgcttttc ttgcgcaaca aatccgcttt acggatatcg ctgcgttgaa tttatccgta
    36601 ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg atgtgttatc tgttgatgcg
    36661 aacgcgagtg aagtcgccag aaaagaggtg atgcgtctcg caagctgagg ataatccggc
    36721 tacagagagt cgcgctattt gttagcgtag ggcttcagtg atatagtctg cgccatctga
    36781 tcgtaagtag ttggctttat aaggtcaggt atgccgtggt tttacacggc ttttttttgt
    36841 ataggcttca gtattcctga gtaccgtaaa ccctgtcagg gaataaaaaa cgcgtgatgt
    36901 tgtctgctac tcaaccactt agcgaaaaat tgccagcgca tggctgccgt catgttgcga
    36961 tcattatgga cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt gcctttgggc
    37021 ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac aacggtattg
    37081 aggcgttaac gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg caggaagtca
    37141 gtgcgttaat ggaactgttt gtgtgggcgc tcgatagcga agtaaaaagt ctgcaccgac
    37201 ataacgtgcg tctgcgtatt attggcgata ccagtcgttt taactcgcgt ttgcaagaac
    37261 gtattcgtaa atctgaagcg ctaacagccg gaaataccgg tctgacgctg aatattgcgg
    37321 cgaactacgg tggacgttgg gatatagtcc agggagtcag gcaactggct gaaaaggtgc
    37381 agcaaggaaa cctgcaacca gatcagatag atgaagagat gttaaaccag catgtctgta
    37441 tgcatgaact ggcccctgta gatttagtaa ttaggactgg gggagagcat cgcattagta
    37501 actttttgct ttggcaaatt gcctatgccg aactttactt tacagatgtt ctctggcccg
    37561 atttcgatga acaagacttt gaaggggcgt taaatgcctt tgctaatcga gagcgtcgtt
    37621 tcggcggcac cgagcccggt gatgaaacag cctgatgggg gtcgcttttg ctgaagtatc
    37681 gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc ggcgttgttt ctgttgccgc
    37741 cggtggggtt cgccattgta acgctggtgg tctgcatgct ggcagcgtgg gaatggggac
    37801 agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat
    37861 tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat caaccgctgg
    37921 ttgaaatctc actttgggct tcgttgggtt ggtggattgt cgcgctattg ctggtgctgt
    37981 tttacccagg ttccgcagca atctggcgta actctaaaac attgcgcctt atttttggcg
    38041 tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg cactatgacg
    38101 agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta tggggcgctg
    38161 actccggcgc atatatgttt ggcaaattgt ttggtaaaca taagctggca ccgaaggttt
    38221 ctccgggtaa aacctggcaa ggctttatag gtggactcgc tactgcagcg gtaatctcat
    38281 ggggttatgg catgtgggcg aatctcgacg tcgctcccgt caccttactc atttgctcta
    38341 ttgtcgcagc gttagcctca gtgctcggcg atctgaccga gagtatgttt aagcgtgaag
    38401 caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta gatcgtattg
    38461 atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta ttcaggacgc
    38521 tttaacggaa ggtaatatgc tgagttttct ctgggatttg gcttcgttca tcgttgcact
    38581 gggtgtactt atcaccgtgc atgaatttgg tcatttctgg gttgcccggc gttgtggtgt
    38641 tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa ctgataagct
    38701 cggcaccgaa tatgttatcg ccctgatccc gttgggcggt tatgtcaaaa tgctggatga
    38761 gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc ttcaataata aatctgtcgg
    38821 ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg ctatctttgc
    38881 ctactggctg gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg gcgaaatagc
    38941 agccaattcg atagctgcgg aagcacaaat tgcaccaggt acggaactaa aagccgtaga
    39001 tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag ttggtcgata aaattggcga
    39061 tgaaagcacc accattacag tagcgccatt tggcagcgac caacggcggg atgtaaagct
    39121 cgatttacgt cactgggcgt ttgagcctga taaagaagat ccggtatctt cgctggggat
    39181 tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa actcggcggc
    39241 aagcaaggca ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc agcccttaac
    39301 gcagtgggtg acctttgtga tgcttgtccg ggataacccg ggtaaatcct tagcgttaga
    39361 aatcgaaagg caggggagtc ccttgtcttt gacattaatc ccggggagta aaccgggtaa
    39421 tggtaaagcg attggttttg tcggtattga gccgaaagtc attcctttgc cagatgagta
    39481 taaagttgta cgccagtatg ggccgttcaa cgccatcgtc gaagccacgg acaaaacgtg
    39541 gcagctgatg aagctgacgg tcagtatgct gggaaaattg atcaccggtg atgtgaaact
    39601 gaacaacctc agtgggccga tctctatcgc caagggggct gggatgacag cggaactcgg
    39661 ggtagtttat tacctgccgt ttcttgcgct tattagcgtg aacttaggga taattaacct
    39721 gtttccgttg cccgtacttg acggggggca tctgctgttc cttgcgatcg aaaagatcaa
    39781 gggcggaccg gtatccgagc gggttcaaga cttttgttat cgcattggct cgattctgct
    39841 ggtgctgtta atggggcttg cacttttcaa tgatttctct cggttatgag agttagttag
    39901 gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct gctgtttagc
    39961 agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt cgaaggcctt
    40021 cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgtacagg cgacacggtt
    40081 aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa ctttgaggat
    40141 gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg tccgaccatt
    40201 gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa gcaaaacctc
    40261 gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc cgatatcgag
    40321 aaaggtctgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt taaagctgtc
    40381 gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga aggtgtgtca
    40441 gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga cgaactgatc
    40501 tctcatttcc aactgcgtga cgaagtgccg tggtggaatg tggtaggcga tcgtaaatac
    40561 cagaaacaga aactggcggg cgatcttgaa accctgcgca gctactatct ggaccgcggt
    40621 tatgcccgtt tcaacatcga ctctacccag gtcagtctga cgccagataa aaaaggtatt
    40681 tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc tttctggcgt tgaagtgagc
    40741 ggcaaccttg ccgggcactc cgctgaaatt gagcagctga ctaagatcga gccgggcgag
    40801 ctgtataacg gcactaaagt gaccaaaatg gaagatgaca ttaaaaagct tctcggtcgc
    40861 tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc cgacaaaacc
    40921 gttaaattac gcgtgaacgt tgatgtgggt aaccgtttct acgtgcgtaa gatccgtttt
    40981 gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca gatggaaggt
    41041 gcatggttgg ggagcgatct ggtcgatcag ggtaaggagc gtctgaatcg tctgggcttc
    41101 tttgaaactg tcgataccga tacccaacgt gttccgggta gcccggacca ggttgatgta
    41161 gtctacaagg tgaaagaacg taacactggt agcttcaact tcgggattgg ttacggtact
    41221 gaaagtggcg tgagcttcca ggctggcgtg caacaggata actggttagg tacaggttat
    41281 gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact gtcggtaacc
    41341 aacccgtact tcaccgtaga tggcgtaagc ctcggtggtc gtctcttcta taatgacttc
    41401 caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac agacgtgacg
    41461 ttgggcttcc cgattaacga atataactcg ctgcgtgcag gtctgggtta tgtacataac
    41521 tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt atctgtactc tatgggtgaa
    41581 catccgagca cctctgatca ggataacagc ttcaaaacgg acgacttcac gttcaactat
    41641 ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc acgtgtcaac
    41701 ctgaccggta aagtgaccat tcctggatcg gataacgaat actacaaagt gacgttagac
    41761 acggcgactt atgtgccgct cgatgacgat cacaaatggg ttgttctggg tcgtacccgc
    41821 tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctacgagaa cttctatgcc
    41881 ggtggttcca gcaccgtgcg tggcttccag tccaatacca ttggtccgaa agcagtttac
    41941 ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc gactcaggac
    42001 ggcgcgaaag acctgtgtaa atcggatgat gctgtaggcg gtaacgccat ggcggttgcc
    42061 agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa ctcggttcgt
    42121 acttcattct tctgggatat gggtaccgtt tgggatacaa actgggattc cagccaatat
    42181 tctggatatc cggactatag tgatccaagc aatatccgta tgtctgcggg tatcgcatta
    42241 caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt caaaaagtac
    42301 gatggagaca aggcagaaca gttccagttt aacatcggta aaacctggta agtgttctcc
    42361 acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa gatcgccggg
    42421 ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa
    42481 gtggttatta gctgcaggtc ttggtttagc actggcaact tctgctcagg cggctgacaa
    42541 aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc
    42601 taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga
    42661 tctgcaggct aaaatgaaaa aactgcagtc catgaaagcg ggcagcgatc gcaccaaact
    42721 ggaaaaagac gtgatggctc agcgccagac ttttgctcag aaagcgcagg cttttgagca
    42781 ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta tccagactgc
    42841 tgtgaaatcc gttgccaaca gccaggatat cgatctggtt gttgatgcaa acgccgttgc
    42901 ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg ttaaataagt
    42961 aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac tacacggtga
    43021 tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag gtcacattac
    43081 gttcatggtt aacccaaaat accgtgagca tttaggcttg tgccaggcgt ccgcggttgt
    43141 catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta
    43201 cctgacttac gcgcgcatgg cgcaaatttt agataccacg ccgcagcccg cgcagaacat
    43261 tgcacccagt gcggtgatcg acgcgacggc gaagctgggt aacaacgtat cgattggcgc
    43321 taacgcggtg attgagtccg gcgttgaact gggcgataac gtgattatcg gtgccggttg
    43381 cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat
    43441 ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag tggtaggcgc
    43501 agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac agattggtcg
    43561 cgtaattatt ggcgatcgcg tggagatcgg tgcctgcaca accatcgatc gcggcgcgct
    43621 ggatgacact gttattggca atggcgtgat cattgataac cagtgccaga ttgcacataa
    43681 cgtcgtgatt ggcgacaata cggcggttgc cggtggcgtc attatggcgg gcagcctgaa
    43741 aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata tggaaatatg
    43801 cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt
    43861 ctattcctca ggcattccgc tgcaatccaa caaagtctgg cgcaaaaccg ctgcactggt
    43921 gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca
    43981 agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc ttacgattgc
    44041 ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat cttgactact
    44101 aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg tttcccgttc
    44161 ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat
    44221 gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat tttcccgggt
    44281 gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa aagcgtagga
    44341 aaactggaac cgggtgagct gtactacttc gctggtattg acgaagcgcg cttcaagcgc
    44401 ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc
    44461 ctgacccgtt ttaaaggggt tgctctggtc gatggtaaag tagtttgcga agcaacgatg
    44521 atgtgtgctc gtagccggga ggcctgatac gtgattgata aatccgcctt tgtgcatcca
    44581 accgccattg tggaagaggg cgcgtcaatt ggcgcgaacg cacacattgg tcctttttgt
    44641 atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg
    44701 aatggtcata ctaaaattgg ccgcgataat gagatttatc agtttgcctc catcggcgaa
    44761 gttaaccagg atttgaaata tgctggcgaa ccgacccgtg tggaaatcgg cgatcgtaac
    44821 cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg attgacgaag
    44881 gtgggcagcg acaacttact gatgatcaac gcgcacattg cgcacgattg tacggtaggt
    44941 aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc acgtatcggt tgacgacttc
    45001 gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc gcatgtgatg
    45061 gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc gcagggtaac
    45121 cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt cagccgtgag
    45181 gcgattaccg ctatccgcaa tgcgtataag ctaatttatc gtagcggtaa aacgctcgat
    45241 gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa agcctttacc
    45301 gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaccag cgtccattaa
    45361 cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt ttaatccgcg
    45421 ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg
    45481 ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc ggtgatgggc attgttgaag
    45541 tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga tctgacaaag cgttttggcg
    45601 aactgaagcc agatgttttt gttggtattg atgcgcccga cttcaatatt actcttgaag
    45661 gtaacctcaa aaagcagggt atcaaaacca ttcattacgt cagtccatcc gtctgggcgt
    45721 ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc gcatttctgc
    45781 ctttcgaaaa agcgttttat gacaaataca atgtaccgtg ccgctttatc ggtcatacca
    45841 tggctgatgc catgccgtta gatccagata aaaatggtgc ccgtgatgtg ctggggatcc
    45901 cttacgatgc ccactgtctg gcattgttgc cgggcagccg tggtgcagaa gtcgaaatgc
    45961 ttagtgccga tttcttgaaa actgcccagc ttttgcgcca gacatatccg gatctcgaaa
    46021 tcgtggtgcc gctggtgaat gccaaacgcc gcgagcagtt tgaacgcatc aaagctgaag
    46081 tcgcgccaga cctgtcggtt catttgctgg atggaatggg ccgtgaggcg atggtcgcca
    46141 gcgatgcggc actactggcg tcggggacgg cagccctgga gtgtatgctg gcgaaatgcc
    46201 cgatggtggt gggatatcgc atgaagcctt ttaccttctg gttggcgaag cggctggtga
    46261 aaactgatta tgtctcgctg ccaaatctgc tggcgggcag agagttagtc aaagagttat
    46321 tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct gttaccgctg ttggcgaacg
    46381 ggaaaaccag ccacgcgatg cacgatacct tccgtgaact gcatcagcag atccgctgca
    46441 atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg aatttgttta
    46501 tccgcacacg cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc
    46561 ggtcgtcacc gctgcggtga tccttgaccc ggcgcgcccg attgccgggc tgaatgattc
    46621 caaaaagctg agcgaaaaac gccgtctggc gctctatgaa gagatcaaag agaaagcgtt
    46681 gagctggagt ctgggccgcg cggaacccca cgaaatcgac gagctgaaca ttcttcatgc
    46741 gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat attgcgccgg aatatgtgtt
    46801 gattgatggt aaccgctgcc cgaaattacc gatgcctgcg atggctgtgg tgaaagggga
    46861 tagccgcgta ccagaaatca gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga
    46921 aatggcggcg ctggatattg ttttcccgca atatggtttt gcccaacata aagggtatcc
    46981 aaccgctttt catctggaaa aactggctga atatggcgcg actgaacatc atcgtcgcag
    47041 ctttgggcct gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc gagattaagt
    47101 aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta
    47161 ctcgatgatc gatggcttgg ccaaaaccgc accgctggta aaaaaggcgg cggcgttggg
    47221 tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga agttctacgg
    47281 agcgggacat ggcgcaggga ttaagcctat cgtcggggca gattttaatg tccagtgcga
    47341 cctgctgggt gatgagttaa cccacctgac ggtactggcg gcgaacaata ccggctatca
    47401 gaatctggcg ttgctgatct caaaagcgta tcagcgcggg tacggtgccg ccgggccgat
    47461 catcgatcgc gactggctta tcgaattaaa cgaagggttg atccttcttt ccggcgggcg
    47521 catgggcgac gtcggacgca gtcttttgcg tggtaacagc gcgctggtag atgagtgtgt
    47581 cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc gcaccggcag
    47641 gccggatgaa gaaagctatc tgcacgcggc ggtggaactg gcggaagcgc gcggtttgcc
    47701 cgtcgtggcg actaacgatg tgcgctttat cgacagcagc gactttgacg cacacgaaat
    47761 ccgcgtcgcg atccacgacg gctttaccct cgacgatcct aaacgcccgc gtaactattc
    47821 gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag ctgtttgccg acatcccaga
    47881 agcccttgcc aacaccgttg agatcgccaa acgctgtaac gtaactatgc gtcttggtga
    47941 atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc tggtcaagcg
    48001 tgcaaaagaa ggcctggaag agcgtctggc atttttattc cctgacgagg aagaacgtct
    48061 taaacgccgc ccggaatatg acgaacgtct ggagactgaa cttcaggtta tcaaccagat
    48121 gggcttcccg ggctacttcc tcatcgttat ggaatttatc cagtggtcga aagataacgg
    48181 cgtaccggta gggccaggcc gtggctccgg tgcgggttca ctggtggcct acgcgctgaa
    48241 aatcaccgac ctcgatccgc tggaatttga cctgctgttc gaacgtttcc ttaacccgga
    48301 acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg atcaggttat
    48361 cgaacacgtg gcggacatgt acggtcgtga tgcggtatcg cagattatca ccttcggtac
    48421 gatggcggcg aaagcggtga tccgcgacgt aggccgcgtg ctggggcatc cgtacggctt
    48481 tgtcgaccgt atctcgaaac tgatcccgcc cgatccgggg atgacgctgg cgaaagcgtt
    48541 tgaagccgag ccgcagctgc cggaaatcta cgaagcggat gaagaagtta aggcgctgat
    48601 cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt
    48661 ggttatcgcg ccgaccaaaa ttaccgattt tgcgccgctt tattgcgatg aagagggcaa
    48721 acatccggtc acccagtttg ataaaagcga cgttgaatac gccggactgg tgaagttcga
    48781 cttccttggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga tcaacaagcg
    48841 gcgggcgaag aatggcgagc caccgctgga tatcgccgcg atcccgctgg atgacaagaa
    48901 aagcttcgac atgctgcaac gctcggaaac cacggcggta ttccagcttg aatcgcgcgg
    48961 catgaaggac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga tcgcactggt
    49021 ggcactgttc cgccctggtc cgttgcagtc agggatggtg gataacttta tcgaccgtaa
    49081 acatggtcgt gaagagatct cctatccgga cgtgcagtgg cagcatgaaa gcctgaaatc
    49141 ggtactggag ccaacctacg gcattatctt gtatcaggaa caggtcatgc agattgccca
    49201 ggtgctttct ggttataccc tcggtggcgc ggatatgctg cgccgtgcga tgggtaagaa
    49261 aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag aaaagaacgg
    49321 aatcaacgcc gaactggcga tgaaaatctt cgacctggtg gagaaattcg cgggttacgg
    49381 atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt tatggctgaa
    49441 agcgcactat ccggcggagt ttatggcggc ggtaatgacc gccgatatgg acaacaccga
    49501 gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga
    49561 tataaactcc ggtctttacc atttccacgt caacgacgac ggcgaaatcg tgtatggtat
    49621 tggcgccatc aaaggggtag gtgaaggtcc gattgaggcc atcatcgaag cccgtaataa
    49681 aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt accgacacca aaaagttaaa
    49741 ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg
    49801 cgcggcgctg atgaactcgc tgggtgatgc gttaaaagcg gcagatcaac acgcgaaagc
    49861 ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg aacaaattga
    49921 acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtattagatg gggaacgtga
    49981 aacgttaggc ctgtacctga ccggacaccc tatcaaccag tatttaaaag agattgagcg
    50041 ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta aagtcatcac
    50101 ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg
    50161 tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta ctgacgccct
    50221 ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag
    50281 ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg atattgacga
    50341 agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc aaattgatga
    50401 ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga caattccagt
    50461 acatctctac tatcagaggg cggatgcacg cgcgcggttg cgttttggcg cgacgtggcg
    50521 tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt cggagcaggt
    50581 ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg attttgaaca
    50641 gccgattgca gagctggaag cgaaaatcga ttctctgact gcggttagcc gtcaggatga
    50701 gaaactggat attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact
    50761 gacgcgtaaa atcttcgccg atctcggtgc atggcagatt gcgcaactgg cacgccatcc
    50821 gcagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg acgaactggc
    50881 tggcgaccgt gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg
    50941 tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg
    51001 taactttggt atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc
    51061 tgaacgcttt aagatgccta tcatcacctt tatcgacacc ccgggggctt atcctggcgt
    51121 gggcgcagaa gagcgtggtc agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg
    51181 ccttggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggcg gtgcgctggc
    51241 gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg ttatctcgcc
    51301 ggaaggttgt gcgtccattc tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc
    51361 gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca tcatcccgga
    51421 accgctgggc ggcgctcacc gtaacccgga agcgatggcg gcatcgttga aagcgcaact
    51481 gctggcggat ctggccgatc tcgatgtgtt aagcactgaa gatttaaaaa atcgtcgtta
    51541 tcagcgcctg atgagctacg gttacgcgta attcgcaaaa gttctgaaaa agggtcactt
    51601 cggtggccct tttttatcgc cacggtttga gcaggctatg attaaggaag gattttccag
    51661 gaggaacaca tgaacatcat tgccattatg ggaccgcatg gcgtctttta taaagatgag
    51721 cccatcaaag aactggagtc ggcgctggtg gcgcaaggct ttcagattat ctggccacaa
    51781 aacagcgttg atttgctgaa atttatcgaa cataaccctc gaatttgcgg cgtgattttt
    51841 gactgggatg agtacagtct cgatttatgc agcgatatca atcaacttaa tgaatatctc
    51901 ccgctttatg ccttcatcaa cacccactcg acgatggatg tcagcgtgca ggatatgcgg
    51961 atggcgctct ggttttttga atatgcgctg gggcaggcgg aagatatcgc cattcgtatg
    52021 cgtcagtaca ccaacgaata tcttgataac attacgccgc cgttcacgaa agccttgttt
    52081 acctacgtca aagagcggaa gtacaccttt tgtacgccgg ggcatatggg cggcaccgca
    52141 tatcaaaaaa gcccggttgg ctgtctgttt tatgattttt tcggcgggaa tacccttaag
    52201 gctgatgtct ctatttcggt caccgagctt ggttcgttgc ttgaccacac cgggccacac
    52261 ctggaagcgg aagagtacat cgcgcggact tttggcgcgg aacagagtta tatcgttacc
    52321 aacggaacat cgacgtcgaa caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg
    52381 ctgttgatcg accgcaactg ccataaatca ctggcgcatc tgttgatgat gaacgatgta
    52441 gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga ttctgggggg gatcccgcgc
    52501 cgtgaattta ctcgcgacag catcgaagag aaagtcgctg ccactacgca agcacaatgg
    52561 ccggttcatg cggtgatcac caactccacc tatgatggtt tgctctacaa caccgactgg
    52621 atcaaacaga cgctggatgt cccgtcgatc cacttcgatt ccgcctgggt gccgtatact
    52681 cattttcatc caatctacca gggtaaaagt ggtatgagcg gcgagcgtgt tgcgggaaaa
    52741 gttttcttcg aaacgcaatc gacccacaaa atgctggcgg cgtcatcgca ggcttcactg
    52801 atccacatta aaggcgagta tgacgaagag gcgtttaacg aagcctttat gatgcatacc
    52861 accacctcgc ccagttatcc cattgttgct tcggttgaga cggcggcggc gatgctgcgt
    52921 ggtaatccgg gcaaacggct gattaaccgt tcggtggagc gggcgctgca ttttcgcaaa
    52981 gaggtccagc ggctgcggga agagtctgac ggttggtttt tcgatatctg gcaaccgccg
    53041 caggtggatg aagccgaatg caggcccgtt gcgcctggcg aacaatggca cggctttaac
    53101 gatgcggatg ccgatcatat gtttctcgat ccggttaaag tcactatttt gacaccgggg
    53161 atggacgaac agggcaatat gagtgaggag gggatcccgg cggcgctggt agcaaaattc
    53221 ctcgacgaac gtgggatcgt agtagagaaa accggccctt ataacctgct gtttctcttt
    53281 agtattggca tcgataaaac caaagcaatg ggattattgc gtgggttgac ggaatttaag
    53341 cgctcttacg atctcaacct gcggatcaaa aatatgctgc ccgatctcta tgctgaagat
    53401 cccgatttct accgcaatat gcgtattcag gatctggcgc aagggatcca taagctgatt
    53461 cgtaaacacg atctttccgg tttgatgttg cgggcattcg atactttgcc ggaaatgatc
    53521 atgacgccac atcaggcatg gcagagacag attaaaggtg aagtagaaac cattgcgctg
    53581 gaacaactgg tcggtagagt atcggcaaat atgatcctgc cttatccacc gggcgtaccg
    53641 ctgttgatgc caggagaaat gctgaccaaa gagagccgca cggtactcga ttttctactg
    53701 atgctttgtt ccgtagggca acattacccc ggttttgaaa cggatattca cggcgcgaaa
    53761 caggacgaag acggcgttta ccgcgtacga gtcctaaaaa tggcgggata acttgccaga
    53821 gcggcttccg ggcgagtaac gtgctgttaa caaataaagg agacgttatg ctgggtttaa
    53881 aacaggttca ccatattgcg attattgcga cggattatgc ggtgagcaaa gctttctact
    53941 gcgatattct tggtttcacg ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga
    54001 aaggggattt ggcgcttaat gggcaatatg tgattgagct tttctctttt ccgttcccgc
    54061 cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca tctggctttt agcgttgatg
    54121 acatcgatgc ggcagtggcg caccttgaaa gccataacgt gaagtgtgaa gccatccgtg
    54181 tcgatccata cacgcaaaaa cgcttcacct tctttaacga tccggacggg ctgccgttgg
    54241 aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg
    54301 ctactgtaaa atcgcaccat catgacactc acgctcaata gacaacttct cacctcacgc
    54361 cagattctgg tggcctttag cggcgggctt gactccaccg ttctgctgca tcagttggtg
    54421 cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta ttcatgtgca tcacggttta
    54481 agtgccaatg ccgatgcctg ggttacgcat tgcgaaaacg tctgccaaca gtggcaggtg
    54541 ccgctggtgg tcgaacgcgt acaacttgcg caagaggggc tggggattga ggcccaggcg
    54601 cggcaggcac gttatcaggc atttgcccgt accctattgc ccggcgaagt gctggtcacc
    54661 gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc
    54721 cctgccgggc tttcggctat ggcggaagtt tcggagtttg ctggaacacg gcttatccgc
    54781 ccgttgcttg cccgcacgcg gggggaactg gagcagtggg cattggcgca tggtttacgc
    54841 tggattgaag acgaaagtaa tcaggacgac tcatacgatc gtaactttct gcgcctgcgc
    54901 gtagtgccgt tattgcagca gcgttggccg cattttgcgg aagcaacggc ccgcagcgcc
    54961 acactttgtg ctgaacaaga gagtctgttg gatgaactgc tggcggatga tttagcacac
    55021 tgccaaacgt cgcaggggac gctgcagatt gcgccaatgc tggcgatgag tgatgcccgt
    55081 cgcgcggcga ttattcgccg ctggctggca gggcagaatg caccgatgcc ttcccgcgac
    55141 gcgctggtgc gcatctggca ggaagtggcg ctggcgcggg aagatgcttc cccctgttta
    55201 cgtttgggcg cctttgaaat ccgccgctat cagtcgcaac tgtggtggat taaatccgtc
    55261 accgggcaaa gcgaaaccat tgtgctgtgg cagacgtggc tacaaccgct ggaattaccg
    55321 gcggggctgg gaaccgtaca gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac
    55381 gaagcggtca gcgtgcgttt caaagcgcca ggattgctgc atattgtcgg gcgcaacggc
    55441 ggacgtaagc tgaagaaaat ctggcaagag ttaggcgtgc cgccgtggct acgtgacacc
    55501 acgccactgc tgttttatgg agaaacgctg attgcggcgg caggggtatt tgtgacgcaa
    55561 gaaggtgtgg ctgaaggtga gaatggcgta agttttgtct ggcagaaaac gcttagttaa
    55621 gtgaaagccg gataagacgc atcaaacgtc gcatccggcg aaagtcaatc aggattcgct
    55681 taccaccacc gtaccgattt cagggtggct aaagctggta attttatcca gacgcagctc
    55741 gcgggtttcg ccagcggcct cgacgaccag atactccaca tttttgcggg agactaaatc
    55801 actggctttt gcctgcaatt tttcgccatc tttcagctca agtgtcagca ttaaatgatg
    55861 ctggcaggcg agctcaagat tatcgtaatc atcacaatta attggttgat acgtatcatt
    55921 cattgacata atcgctcacc agtaagtttg ccgcagcgta tgctgctttt tccctgacag
    55981 cctcagaaag ggcgtcgtcg gcagccattt cattcagcac tttcaaaacg cagcccagcg
    56041 cgtccggaac gtatcctaag tctccgctgg cgatttccgc gtaccgcttg cgtattaact
    56101 cacaatattt ttccacatgc cctcctgtca gcactctgac ttaaccgtgg atgcaagtct
    56161 aagcctacga agataaactc tgtttcgcaa ggtgactata ccacactcat ttctgcaata
    56221 tcagcgccgc aactgcacgt attccgttac aatggcctcc tgattcgaaa ggagttttct
    56281 tatggcgctt aaagcgacaa tttataaagc gacggttaat gtggccgatc tcgaccgcaa
    56341 ccagtttctc gatgcctctc tgacgctggc gcgccaccct tcagaaaccc aggagcgtat
    56401 gatgctgcgc ttgctggcgt ggctgaaata tgctgatgaa cgtctgcaat ttacccgtgg
    56461 tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac gatcacctgg gcattgattt
    56521 gtggattgag ctggggctgc cggatgagcg acgaattaag aaagcctgca cccaggccgc
    56581 agaagtggcg ctgtttacct ataatagtcg ggcggcgcaa atctggtggc agcaaaatca
    56641 gagcaaatgt gtgcagtttg ccaatctttc cgtctggtat ctggacgatg aacaactggc
    56701 gaaagtaagc gcctttgccg atcgtaccat gacgctgcag gcaacgattc aggatggtgt
    56761 tatctggtta tcggatgata agaataatct ggaagttaac ttaaccgcct ggcaacaacc
    56821 ttcatgattg tgatttcccg acatgttgct attcccgatg gtgagcttga gatcaccgcc
    56881 attcgtgcgc agggcgctgg cgggcagcat gttaataaga cctcaacggc tattcatctg
    56941 cgttttgaca ttcgggcgtc cagcctgcca gagtattaca aagagcgtct gctcgccgcc
    57001 agccatcatt tgatcagcag tgatggcgtg attgtcatta aggcacagga ataccgcagt
    57061 caggaactga accgcgaagc cgctctggcc cggctggtgg cagtgattaa agatttaaca
    57121 aaagaacaaa aagcccgacg acccacgcgg cccacccgtg catcgaaaga gcgcaggctg
    57181 gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg gcaaagtacg cagcggtcgg
    57241 gaataaaaag aaggaatgga tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag
    57301 cctctttact ctgatgggat gtaataatcg ggccgaagtc gatacgcttt ctccggcgca
    57361 ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc gtgctaccgt gtgccgattg
    57421 cgaaggaatc gaaatctctc tgttcctcga aaaagacgga acatgggtga tgaatgagcg
    57481 ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc tacggtacat gggcgcgaac
    57541 cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag tcatattatc gggcgaaagg
    57601 cgatgcgctg gagatgctcg atcgtgaagg caatccaatt gaatcgcagt tcaactatac
    57661 gctggaaccg gcacaatcca gtttacctat gacgccgatg accctgcgag gcatgtattt
    57721 ttatatggct gatgcggcga ccttcactga ttgcgcgacc ggaaaacgtt tcatggtagc
    57781 gaataacgca gagctggagc gtagctacct ggctgcgcgc ggtcacagtg aaaaaccggt
    57841 gttactgtca gtagaaggtc actttacgct tgaggctaat ccggatactg gtgcgccaac
    57901 caaagtatta gcgcccgata cggcgggtaa attttacccg aataaggatt gcagtagttt
    57961 ggggcagtaa acctgcttaa ggcagaaaca aacgcaaaat tgcctgatgc gctgcgctta
    58021 tcaggcctac agcatataat gcaacatttt gaattagcac gattttgtag gccggataag
    58081 gcgtttacgc cgcatccggc attttgtgca cgaggctggc tttatcactg agtcagccca
    58141 acaaagcgac ccgccttgat ataaatccca ggcttcaggt gcccaacata ctgtaactgc
    58201 gtttctggct taaacgcaga gacatcacca atacgcatat gcatcagatc ggcagggcta
    58261 atccagcccg attgcgccag tgtaagtgga tgcccggatt tggcaaatgc atcagtgaca
    58321 aattccgaac aaaaccacga ctttttgtct ccttcgcaca cactgcttaa ctgcgctttc
    58381 gccaggtcgc tgacgcactg ttggcgaaaa tcctcggaga acggattcag tgagcacatc
    58441 tggcgagtca ccataaaggg aataaattcg acaatgccgc gatagttata accgctatct
    58501 ttgattttat tggcaaacgc ggtgatatct gtggcttgtt gcggggtaag atccgggact
    58561 cgtaagacga aaagcttatc actatgcttc atcgcttttt taagggaaac aatctggacg
    58621 ccagcgcctg tcgcttctgc aacgttatta tcacccagaa agattgcaac gtgactcaca
    58681 gaggaagtgc tgaagacgcg gattccaaat gaggttaccc caaggcttga ggagaacagc
    58741 aaatcgccgg gtttgagatc aggtgccgtt atttctttta ttgattgttc ggtgaaagag
    58801 ctttgatgct ggaatttgac agcccatgtt tttgcctcgg catctactgc tgtggctgat
    58861 gggtctggct ggctgatatc aacggtacaa gccgaaagta aaagaaagct ggggagaagc
    58921 aggcggcagt acgcctttgg tttatccatt ttatacaatc catgtaaaaa agggccctga
    58981 aattcaggac cctttctggc atcagccttt aatctgtttc accagatatt cgacgatgtc
    59041 accagtctta attaactgtt tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt
    59101 gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt ccatatcagc
    59161 aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac cttgtgcacg
    59221 cagttcgctg tacagtttct cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat
    59281 cggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc
    59341 gtagttctgc tcaatcgccg cagctaccac acgcgttacc ccgataccgt agcaacccat
    59401 cgtcaggatt tggttacggc catcttcacc ctgtacggag gctttcagtg cttcggagta
    59461 cttggtaccc agctggaaga tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc
    59521 ctggccatcc gggcttggat cgccagccac cacgttacgg atatctgcaa cttccggggt
    59581 agcgacatcg cgatcccagt tgataccgaa gtagtgttta ccatcgatgt tagcaccagc
    59641 agcgaaatca ctcatcgccg caacggtacg gtcaatcacc accggaatcg gcatgtttac
    59701 cggacccagt gaacccggac cggctttaac cacagcacga atttcttctt cggtcgcgaa
    59761 agtcagcggg cttgcaacct gcggcagttt ttctgcttta acttcgttca gctcgtgatc
    59821 accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaaccgctt taaccagcag
    59881 agtcttaacc gttttctcaa tcggcagatt gaactgttca accagttccg cgatggtttt
    59941 cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg
    60001 cgcgatagct tctgccagtt caatgttcgc tgcatagtca gaggtgtcgg agaagaccac
    60061 atcgtcttca ccgctctgcg ccagcacctg gaattcgtga gaggcgctgc cgccgataga
    60121 accggtgtcg gcctgtacgg cgcggaaatc cagccccatg cggctgaaga ttttgctgta
    60181 ggccgcatac attgcatcgt aggtttcctg cagggattcc tgagaagtat ggaaagagta
    60241 agcatctttc atcaggaatt cgcgggaacg catgacgccg aaacgcggac gcacttcgtc
    60301 gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag agctaagctc
    60361 gttacgaatc aggtcagtga taacttcttc atgagttggg ccgagtacga acggacgctc
    60421 gccacggtca acaaaacgca gcagttccgg accgtactgt tcccaacgac cactctcttg
    60481 ccacaaatcg gctggctgaa ccaccggcat cgacacctcg atcgcaccgg cgttgttcat
    60541 ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg gcagccaggt
    60601 atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct gatggctgat
    60661 cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc tagtacgcat
    60721 gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg acaaaaaaag
    60781 tgatttagtt taccagtgca aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt
    60841 tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt
    60901 agccagacgg cataggtttt gcccgtttcc tcacctttac gataggccgg gcgcgggtcc
    60961 tgcgccagta cttcgcggat aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa
    61021 agctgctttt cgacctctgc ggtaaaactc accgccatct ctgcagctgg cgcactttgc
    61081 gcatagctgg cactggcatc gggaagcgat tcggcaaagg ggagatacgg tttgatatcc
    61141 actaccggcg taccatcgac cagatccaga ctgccgagct tcagaatcac gctgccttta
    61201 tggcaaacaa cctctttcag ctctaccagc gacatgccaa ttgggttagg gcggaaagta
    61261 gagcgtgttg cgaaaacccc cattctggcg ttaccgccga ggcgcggtgg acgcacagtc
    61321 ggacgccagc cgccttccat cgtttgatga aagacgaaaa ggatccataa atggctgaat
    61381 gcttccaggc cgcgaacggc gtcggcctgg ttgtaggaag caatgagatg cagttcgccg
    61441 ttggcgcttt ttaccagacc tggctggcgc ggaacggcga atttttcttt atagggcgag
    61501 cgaataacgc ctatttgctc gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag
    61561 agccgataca tacagcctga cgatagcagc ccggcgtacc gctggtgact tcgcagctat
    61621 gcagtaatac agcattggct ttcattttag aggcattgat ttgcatctgc ttacgtgcgg
    61681 ttggaatgct cggcggagag tcctgattag aggcctggca agagtcgcca ctgacttcac
    61741 cgagatcgcg gaacggtttg ccgactaatt cttctgcatt ggtataaatt cggaccggcg
    61801 tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa
    61861 cgggttcgac aggggatctg cttaacatgg aacagccgct tagcatgagt gctactaaac
    61921 agatcggtaa agcacgcata gtatttcctc aatgtatgat caaaacgtca atattgaatc
    61981 aggagcttgt aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt
    62041 ggaaacagcc taaaaattac cagcctttaa cagcgccgcc gttaaacact ttgtttgctg
    62101 cttcgtaaac ttcatcagac tgataagcct ggacgaattt cttcacgttc tcggcgtctt
    62161 tgttatcttc acgcgttacg atcaggttta cgtatgggga gtctttatct tcgacaaaga
    62221 taccgtcttt cgccggagtc aggccaatct ggctggcata ggtggtattg ataactgcca
    62281 gagcgatttg cgcgtcgtcc agagaacgcg gcagttgcgg tgcttccagt tcaacaattt
    62341 tcagattttt tgggttctca acaacatcaa gaacggtcgg cagcaggcca acgccatctt
    62401 tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaaga ttagttggat
    62461 cgtttggcac ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg
    62521 agtaaccagc aatcggataa acaaaagtgt tgcctactgc gatcagtttg tagccacgat
    62581 ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt
    62641 tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt taccagctca acgtccaggc
    62701 catatttgtc tttcgcaact ttctgcgcga cttctgctac ctgctgttcg gcaccaacaa
    62761 tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca
    62821 gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt
    62881 ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc
    62941 ggtcgcctgc gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca
    63001 tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct aaaccaccgg
    63061 caccgactgc accacccatc gcggaataac cgaccagggt aatcagggtg atagttgccg
    63121 cattcaccag acccggcaac gcttccggta acagcacttt acagacgatc tgcattggcg
    63181 tggcacccat tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcgttct
    63241 cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatt gctgcctgca
    63301 aaccaatcga tgtaccgaca ataacgcggg taaacggaat catccatacc agcaagataa
    63361 tgaacgggat ggaacggaaa atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt
    63421 tagcaataat ttgccccgga cgcgtgacat aaagcagaac gccaaccggc agaccaatca
    63481 caaacccaaa aaatccggat acgaaggtca ttgccagcgt ttcccatacg ccacgaacca
    63541 gcagccacat catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc
    63601 agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg
    63661 ccgaatttaa cgccaccggc gtagtccatc tgcgcgctaa taatgttgtt gttgacgttg
    63721 aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga
    63781 cgcagcatcg gcacgcagtc agtaaatggc tccgtttgca gacgttcctg gtaatcttcc
    63841 gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt tttcggatgc
    63901 gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattgctgat gaccgccacg
    63961 caatcacaaa tacgcttcac aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc
    64021 agacggcggt taatgtcttt cagcagttcg agaatagaac gtgtcgttgc cgggtccagc
    64081 gcgctggtgg cttcatcaca cagcaatact ttgggattgc tggctaatgc acgggcaatc
    64141 gccacacgtt gtttctgccc accggaaaga ttcgacgggt agctatcatg cttatcgcca
    64201 agaccaacca atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg
    64261 tccagctcca gcggcagagc cacgttgcca aaaacagtac gcgaagagag caggttaaaa
    64321 tgctggaaaa tcataccaat ctggcggcga gctttggtca actcggattc tgacagcgtg
    64381 gtcaattcct ggccatcgac cagcacgcta ccctcggttg ggcgctccag caggtttaca
    64441 caacgtataa gcgtactctt acccgcgcct gaggcaccga taacgccata aatttgtcca
    64501 gctggcacat gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac
    64561 actttggtga tattcgaaag ttttatcatt gattatttat tatcgtcatt aagttagtcg
    64621 tagcatctcg aatgcctgaa acgggcaacg ccgtcaatga aatggatgtt aaggcatcca
    64681 gacgtctaaa tcaatcaggt ttatgcgaag agcactttct tgcaggtcga aacatgcgat
    64741 actagcgtcc catgccttat taaggagcta taaaaggtgg cgaagagcgt acccgcaatt
    64801 tttcttgacc gtgatggcac cattaatgtc gatcacggct atgtccatga gatcgacaac
    64861 tttgaattta tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg
    64921 ctggtggtag taaccaacca gtctggcatt gctcgcggta aatttaccga agcacagttt
    64981 gaaacgctga ccgagtggat ggactggtcg ctggcggacc gagatgtcga tctggatggt
    65041 atctattatt gcccgcatca tccgcagggg agtgttgaag agtttcgcca ggtctgcgat
    65101 tgccgcaaac cacatccggg gatgcttttg tcagcacgcg attatttgca tattgatatg
    65161 gccgcttctt atatggtggg cgataaatta gaagatatgc aggcagcggt tgcggcgaac
    65221 gtgggaacaa aagtgctggt gcgtacgggt aaacctatta cacctgaagc agaaaacgcg
    65281 gcagattggg tgttaaatag cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa
    65341 ccggcgcaat gattaaaaga tgaacggttg aaataaaaat gcatttttcc gcttgtcttc
    65401 ctgagccgac tccctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca
    65461 aacagccggt tcggttgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga
    65521 aagtgtgata tacgccacct cgcgacagtg agctgaaagc tgcgtcgcaa ctgctcttta
    65581 acaatttatc agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac
    65641 gataaatgaa taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt
    65701 gagcatcaaa cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag
    65761 gcctaacaca tgcaagtcga acggtaacag gaagcgcttg ctgcttcgct gacgagtggc
    65821 ggacgggtga gtaatgtctg ggaaactgcc tgatggaggg ggataactac tggaaacggt
    65881 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
    65941 gatgtgccca gatgggatta gcttgttggt ggggta
//
LOCUS       DAECOD010000008        65507 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.008, whole genome shotgun sequence.
ACCESSION   DAECOD010000008 DAECOD010000000
VERSION     DAECOD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.008"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            131..574
                     /locus_tag="KKO15_000504"
     CDS             131..574
                     /locus_tag="KKO15_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6359430.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="KKO15_000505"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="KKO15_000505"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBD6359431.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KKO15_000506"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KKO15_000506"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBD6359432.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KKO15_000507"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KKO15_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBD6359433.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KKO15_000508"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KKO15_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6359434.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KKO15_000509"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KKO15_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBD6359435.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="KKO15_000510"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="KKO15_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBD6359436.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="KKO15_000511"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="KKO15_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBD6359437.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTM
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="KKO15_000512"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="KKO15_000512"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBD6359438.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTLVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="KKO15_000513"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="KKO15_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBD6359439.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="KKO15_000514"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="KKO15_000514"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBD6359440.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="KKO15_000515"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="KKO15_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBD6359441.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="KKO15_000516"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="KKO15_000516"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBD6359442.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGRAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KKO15_000517"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KKO15_000517"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBD6359443.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="KKO15_000518"
     CDS             13934..14314
                     /locus_tag="KKO15_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBD6359444.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="KKO15_000519"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="KKO15_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBD6359445.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KKO15_000520"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KKO15_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBD6359446.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KKO15_000521"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KKO15_000521"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD6359447.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="KKO15_000522"
     tRNA            complement(17415..17490)
                     /locus_tag="KKO15_000522"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="KKO15_000523"
     tRNA            complement(17494..17569)
                     /locus_tag="KKO15_000523"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="KKO15_000524"
     tRNA            complement(17621..17696)
                     /locus_tag="KKO15_000524"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="KKO15_000525"
     tRNA            complement(17699..17774)
                     /locus_tag="KKO15_000525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="KKO15_000526"
     tRNA            complement(17911..17986)
                     /locus_tag="KKO15_000526"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KKO15_000527"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KKO15_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBD6359448.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KKO15_000528"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KKO15_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBD6359449.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMFSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KKO15_000529"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KKO15_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBD6359450.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22207)
                     /gene="tolA"
                     /locus_tag="KKO15_000530"
     CDS             complement(20933..22207)
                     /gene="tolA"
                     /locus_tag="KKO15_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD6359451.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22272..22700)
                     /gene="tolR"
                     /locus_tag="KKO15_000531"
     CDS             complement(22272..22700)
                     /gene="tolR"
                     /locus_tag="KKO15_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBD6359452.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22704..23396)
                     /gene="tolQ"
                     /locus_tag="KKO15_000532"
     CDS             complement(22704..23396)
                     /gene="tolQ"
                     /locus_tag="KKO15_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBD6359453.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23393..23797)
                     /gene="ybgC"
                     /locus_tag="KKO15_000533"
     CDS             complement(23393..23797)
                     /gene="ybgC"
                     /locus_tag="KKO15_000533"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD6359454.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIANAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23947..24240)
                     /gene="ybgE"
                     /locus_tag="KKO15_000534"
     CDS             complement(23947..24240)
                     /gene="ybgE"
                     /locus_tag="KKO15_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBD6359455.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24240..24353)
                     /gene="cydX"
                     /locus_tag="KKO15_000535"
     CDS             complement(24240..24353)
                     /gene="cydX"
                     /locus_tag="KKO15_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBD6359456.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24368..25507)
                     /gene="cydB"
                     /locus_tag="KKO15_000536"
     CDS             complement(24368..25507)
                     /gene="cydB"
                     /locus_tag="KKO15_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD6359457.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLNRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25523..27091)
                     /gene="cydA"
                     /locus_tag="KKO15_000537"
     CDS             complement(25523..27091)
                     /gene="cydA"
                     /locus_tag="KKO15_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBD6359458.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27940..30573)
                     /gene="mngB"
                     /locus_tag="KKO15_000538"
     CDS             complement(27940..30573)
                     /gene="mngB"
                     /locus_tag="KKO15_000538"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBD6359459.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30591..32507)
                     /gene="mngA"
                     /locus_tag="KKO15_000539"
     CDS             complement(30591..32507)
                     /gene="mngA"
                     /locus_tag="KKO15_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBD6359460.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32677..33399
                     /gene="mngR"
                     /locus_tag="KKO15_000540"
     CDS             32677..33399
                     /gene="mngR"
                     /locus_tag="KKO15_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBD6359461.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33502..34371)
                     /gene="sucD"
                     /locus_tag="KKO15_000541"
     CDS             complement(33502..34371)
                     /gene="sucD"
                     /locus_tag="KKO15_000541"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBD6359462.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34371..35537)
                     /gene="sucC"
                     /locus_tag="KKO15_000542"
     CDS             complement(34371..35537)
                     /gene="sucC"
                     /locus_tag="KKO15_000542"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBD6359463.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35631..36848)
                     /gene="odhB"
                     /locus_tag="KKO15_000543"
     CDS             complement(35631..36848)
                     /gene="odhB"
                     /locus_tag="KKO15_000543"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBD6359464.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36863..39664)
                     /gene="sucA"
                     /locus_tag="KKO15_000544"
     CDS             complement(36863..39664)
                     /gene="sucA"
                     /locus_tag="KKO15_000544"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBD6359465.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39965..40681)
                     /gene="sdhB"
                     /locus_tag="KKO15_000545"
     CDS             complement(39965..40681)
                     /gene="sdhB"
                     /locus_tag="KKO15_000545"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBD6359466.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40697..42463)
                     /gene="sdhA"
                     /locus_tag="KKO15_000546"
     CDS             complement(40697..42463)
                     /gene="sdhA"
                     /locus_tag="KKO15_000546"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBD6359467.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42463..42810)
                     /gene="sdhD"
                     /locus_tag="KKO15_000547"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42463..42810)
                     /gene="sdhD"
                     /locus_tag="KKO15_000547"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBD6359468.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42804..43208)
                     /gene="sdhC"
                     /locus_tag="KKO15_000548"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42804..43208)
                     /gene="sdhC"
                     /locus_tag="KKO15_000548"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD6359469.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43589..43696
                     /locus_tag="KKO15_000549"
     CDS             43589..43696
                     /locus_tag="KKO15_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359470.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43902..45185
                     /gene="gltA"
                     /locus_tag="KKO15_000550"
     CDS             43902..45185
                     /gene="gltA"
                     /locus_tag="KKO15_000550"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBD6359471.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVCTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45576..46142
                     /locus_tag="KKO15_000551"
     CDS             45576..46142
                     /locus_tag="KKO15_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6359472.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46193..48652
                     /locus_tag="KKO15_000552"
     CDS             46193..48652
                     /locus_tag="KKO15_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6359473.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48667..49398
                     /locus_tag="KKO15_000553"
     CDS             48667..49398
                     /locus_tag="KKO15_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD6359474.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49395..50471
                     /locus_tag="KKO15_000554"
                     /pseudo
     CDS             49395..50471
                     /locus_tag="KKO15_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50623..51669
                     /locus_tag="KKO15_000555"
     CDS             50623..51669
                     /locus_tag="KKO15_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBD6359475.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51666..52457)
                     /gene="nei"
                     /locus_tag="KKO15_000556"
     CDS             complement(51666..52457)
                     /gene="nei"
                     /locus_tag="KKO15_000556"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBD6359476.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52493..53227)
                     /gene="pxpA"
                     /locus_tag="KKO15_000557"
     CDS             complement(52493..53227)
                     /gene="pxpA"
                     /locus_tag="KKO15_000557"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBD6359477.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53217..54149)
                     /gene="pxpC"
                     /locus_tag="KKO15_000558"
                     /pseudo
     CDS             complement(53217..54149)
                     /gene="pxpC"
                     /locus_tag="KKO15_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54143..54799)
                     /gene="pxpB"
                     /locus_tag="KKO15_000559"
     CDS             complement(54143..54799)
                     /gene="pxpB"
                     /locus_tag="KKO15_000559"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBD6359478.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54822..55565)
                     /gene="ybgI"
                     /locus_tag="KKO15_000560"
     CDS             complement(54822..55565)
                     /gene="ybgI"
                     /locus_tag="KKO15_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBD6359479.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55836..57317
                     /gene="dtpD"
                     /locus_tag="KKO15_000561"
     CDS             55836..57317
                     /gene="dtpD"
                     /locus_tag="KKO15_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBD6359480.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57467..58885)
                     /gene="phrB"
                     /locus_tag="KKO15_000562"
     CDS             complement(57467..58885)
                     /gene="phrB"
                     /locus_tag="KKO15_000562"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBD6359481.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58882..59391)
                     /locus_tag="KKO15_000563"
     CDS             complement(58882..59391)
                     /locus_tag="KKO15_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBD6359482.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59520..59807
                     /locus_tag="KKO15_000564"
     CDS             59520..59807
                     /locus_tag="KKO15_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBD6359483.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59862..60242)
                     /locus_tag="KKO15_000565"
     CDS             complement(59862..60242)
                     /locus_tag="KKO15_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359484.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60245..64909)
                     /locus_tag="KKO15_000566"
     CDS             complement(60245..64909)
                     /locus_tag="KKO15_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD6359485.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETVFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYRFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDKLGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGRITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPW
                     ET"
     gene            complement(64928..65131)
                     /locus_tag="KKO15_000567"
                     /pseudo
     CDS             complement(64928..65131)
                     /locus_tag="KKO15_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15874 a        17750 c        15991 g        15892 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc aggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactatggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgcttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaga gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaaaacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcttggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagctttct ctgctgccgc tttcttatca gctgcagcct
    21541 tttcagcagc cgctttcttc tcagcttctg ccttggcttt ttcagcagct tcagttgccg
    21601 ctttctttct tgcttcagct gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg
    21661 cagcggctac ctcggctttt ttctgcgctt cggctgcggc tttggcggct tctgcttctg
    21721 cttttttctt cgcgtctgca gccgctttct tcgctgcttc ttccgcagct ttagcatctg
    21781 ccgccgcttt cgctgccgcc acttcagctt gcttctgctt taactcggcc tgttttgcgg
    21841 cttcttcagc ctgctttttc tgctcctgag ccgctaaccg ctctttctca agttgcttca
    21901 ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc tgttccttca
    21961 tcttgcgctg ttcatcagaa cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact
    22021 gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc
    22081 cggctgaagc ttctatattc tcatcgaacg aactccagat cagcgccgca aataagatga
    22141 catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt tcggttgcct
    22201 ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc aagcaaggga
    22261 aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt
    22321 aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc accgatcaga
    22381 aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac ctgctctggt
    22441 ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat accagacact
    22501 tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt agcgtctggc
    22561 agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc
    22621 accagcagta cgtccagcaa cggaacaatg ttgatttcgg acttgagatc gcgacgacct
    22681 cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt aaacgcctgg
    22741 cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc cagtttgttt
    22801 acgcgctggt tgagacggtt ataagccatt accgccggaa ttgcggcgaa cagaccaatt
    22861 gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt
    22921 accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata
    22981 tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag attttcaagt
    23041 tcacggttca tggagatacg catagcacgc gacgcccctt ccactacggc ttccggcgca
    23101 tggctattgg cacgatgcag gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc
    23161 gaaccagtca gattatcccg tttcccctga ctctcttgat agaggcgaga gagttcgatt
    23221 ccagaccaga atttatcttc aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg
    23281 gttcgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat caacatgata
    23341 agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc agtcactgct
    23401 taaactccgc gacaatagac ttgggaagcg cacgaggctt cattttgagt gggtcaacgc
    23461 aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttggca atacgttgcg
    23521 tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg agcatatcgt
    23581 cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag gcaacgcgtt
    23641 cagccatcag cgcctgctga ctgaagtgat gatgacgcag catctctgtg cgtgctcttt
    23701 cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta tcttcatagt
    23761 agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt aatgcaacaa
    23821 aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg gaatgagggc
    23881 aagttaaggg cgcgtaaaaa tttatgggcc tggagcggcc cataattttt taaagagaat
    23941 tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg caagcaatgg
    24001 gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc
    24061 agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac tggtcttcgc
    24121 ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga
    24181 aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa tcttactcat
    24241 cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt
    24301 accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc
    24361 tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga
    24421 acattttcca gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa
    24481 cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt gtcagacttg
    24541 cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg gctgtcagga
    24601 tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta tccatacgtg
    24661 cagtcaggat ggtcagcagc agcagaacca cacccagtgc cggaatagcc cacagaattg
    24721 gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagtg
    24781 ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca
    24841 tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg
    24901 ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag gttgcgccct
    24961 gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcaact
    25021 ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct
    25081 gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaagcta ccaatgaaga
    25141 tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac
    25201 cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaga
    25261 acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc
    25321 aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga cgctcggtgt
    25381 cgttacgacc gaggaaacgg ttgagcatgc ccacccccat gtcgaaaccg tcagtgactg
    25441 caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat
    25501 cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa
    25561 agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga
    25621 acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga
    25681 gatcgcctgc ggtcagtgac gaattcgcca cagctgtcgg cagcacttca ccgatagccc
    25741 acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg
    25801 gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg ttgcggatga
    25861 cactccagaa agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac
    25921 ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc tgttgaatct
    25981 gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccgtaaccga
    26041 ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca
    26101 gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca
    26161 tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg atgcccagtg
    26221 cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca
    26281 aagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca gccagtttgg
    26341 ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa
    26401 cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac
    26461 gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca gtcacataac
    26521 cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga
    26581 agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca
    26641 tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc
    26701 aggtgacaca catatgctga actttaccca gacgatccca accgaagaag aacagaccta
    26761 caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc gcaccgaaga
    26821 tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca
    26881 gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca
    26941 tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg gccaggagga
    27001 acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact
    27061 gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact
    27121 ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat ttgtttgaac
    27181 actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt
    27241 taataaaaac tcaaattgat cccacgtata tattacgccg caaaatcctt acaataaaca
    27301 ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc taaattgccg
    27361 cctttcagcc aatttgatcg agaacaattt atctcttttt gatgcccatt tccaagactt
    27421 atacattgat aaatatcaat ttctacaaat tatgttaatc atgtttcaac aaggtaactt
    27481 tagtgatatt tatgtttaaa atgtgtgaat tataagactg aaattagttt cggttggcaa
    27541 ttaaagataa aaagagacga agcaaaaaca gcacggttga ccttaattct ttttttggtc
    27601 actggctaat acgacgcaga gtttcaggat tattaataaa ggcataaatc tcccccccgc
    27661 cgatattaac atttaattaa cttataatta tattataatt aactcacccg tggttattat
    27721 tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaaca caaaattacc
    27781 tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat ttgcctgctt
    27841 ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc ctttgtcaac
    27901 aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc ggtaactgaa
    27961 cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat tttcctcggt
    28021 ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac
    28081 agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca cttcttgtcg
    28141 gtcttcagct ttcttaagtg cgcttatcag gcatcccact gggggcattt tcaacaaact
    28201 ataactttcc ggcacgttga atccggcttt gttgagcttc atcgcatccc atgggatttt
    28261 gttgtaacac tgtactggag tcagccatgc tcgcgcctgc tgagctacac cagcggcggt
    28321 tggcgtaccg gtataactca ataaacttag gcgacaagaa agcagaccac gtagttgtga
    28381 gtccgggact ggcattttaa ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt
    28441 ccccagtaag cccacgccgc gcagcaacgt aatggcaaag gttttcttct cttcaccgat
    28501 gacttcaaat tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa
    28561 ggctgcatag ttgagcatat tccataccgg aaccggcgct tctttccagc cttcttgctg
    28621 ccagttgttc attgcactgt cgttcacggg gcgcgttagc gaaccaaact gggtatctgc
    28681 cagaacacta tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc
    28741 ctggttatca aggttgatat ccacatcaat gcgcctgttg ttatgactaa gagtgacaac
    28801 caactcgaca tctactctgc cagtggattg tcttgcgctg cgttgttgca gattacgcgg
    28861 caccgccatt tcatagcgga taacagccct gctctgccag gcctcatgaa taatctcgca
    28921 ttgcggtttc gcattcgccg aagtaattac ccactcttct tttgcgggtg aatagtcata
    28981 ttcatcacca tcatcagagc tttcttcaat ttgcaacacc cggtcatagc gcacaccgct
    29041 atctttatct accagttgta gcgttccatc ctcattgagc gcaatttgcc agaaagcatt
    29101 ttccagtatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct gattcgcttc
    29161 gatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgta tatcaaactc
    29221 cataaaggga tcgtaattac cgtaatgaac tatttgccga tcgattaggc ctggatcgat
    29281 ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctc gtagattaaa
    29341 ctggctggcg cgcagccgca cagtggtgtt gatcacttct tcccgcggcc agggcatcag
    29401 gttaaacagg acgagtttgt cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat
    29461 atagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc
    29521 gcgatgaact ttgtcactac agcagcagcc gatgctgtcg tgggcatgat tttttaagat
    29581 ctctttccac attttctcca gcaagccgtg gtggtatcca aaacccaacg tcctggccag
    29641 cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt gggcaacttt
    29701 gatatccatg cgcgtggaac cgatggtgcg atgcacgcgc atatatttgc catcaataaa
    29761 ttcccctttc agggttgcca gattatctcg ctgcgcttcg atcttctcaa atacctcttc
    29821 aaagcggctc atcacaaact ttcgttgagg ataaatttcg cgcagtttct ccatcacttc
    29881 gaagatattt tgctgcaatg gcatctgatc atgcccattc ggcagcaaaa tctctttggt
    29941 tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttgcgta atccgttttc
    30001 gtccgcaggt aagtacttac cgatggcata acccaacggc atcacctgcg ccgtcacttc
    30061 gctaccgtca ctgctttgcc acaaaaactc ggttttatcc gtaccgtggc gctcagaaca
    30121 tccacgccag aacatggtgc aggtaatgcc aaatccattg tagatatgcg gcagttgccc
    30181 ggacatgcca aaggaatcgg gtaaataacc tattttcatc ggctcgccaa acgcgaggca
    30241 gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa tcgtggtatc
    30301 ggtctgggta taccaggggc cgataatcaa cttgccggct tctaccagtt tcttcacgcg
    30361 gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag
    30421 tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt ccatattatt
    30481 gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc agtgcatatg
    30541 cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt ttatggcatt
    30601 acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat caggagaatt
    30661 gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc cgccataaca
    30721 ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa
    30781 aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag cacaaacgaa
    30841 ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc
    30901 agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa caggcgcggt
    30961 gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg
    31021 gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata agcggcttta
    31081 ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc cagcaacagc
    31141 gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat ccaggcgaca
    31201 ggttccccca ccacaaacag catcagactg cccgctccca atgtaccgag caccgggtag
    31261 agataaaaag tcaggaagcc attgaattta ctgctaagac gcaagtgatt tttcatccag
    31321 cgtatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc ggagccgatc
    31381 atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa
    31441 taggcggtat aggctgccag caccggtacc atcaaaattc cgagcagccc gccgcccagt
    31501 ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag cccgaagatt
    31561 tgcgacagta ataccgcgac cgccagcacc gtcccccccg cgacaatcaa cggtacggca
    31621 aaagagatcc cgctcaacag tgcctgtttc agctccgttt tgacgctttt caccggttgc
    31681 gtatcttgct gtaaggtacg cgtctcatcg ccacgctcga gggtaagcga ttgttggatc
    31741 aacgcttctg catggcgaat cggctcggca acaggcacag aaagcgcagg aatgccgtta
    31801 aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcagt tgcactattg
    31861 agttgatccg ccgttaaacg cccttcaata ccgttagcac cttgcttttc aacgtagaca
    31921 ttcacgccga gtttgcgtcc ggctttttcc agatactccg cagccatgta ggtgtgagca
    31981 atacccgccg ggcaggccgt tacgcaaacg atagttggcg cgttggaaaa agaggcagaa
    32041 ggttgcgtgc ctcccttgtc atccagcgcc gagagcaatt catcaggcgt cgtcgccgac
    32101 tgtatacgcg cccgaatctc atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc
    32161 atgtgcgtag tacccgcttc attgagggga atcgccagca acaccactaa atcaaccgct
    32221 tccgggccat ccacgccttc ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc
    32281 gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcact taaggcggtc
    32341 gggccaaggc tttcacggcg atacacttct ttcagaaatt gctcagtact ggaaattttc
    32401 cccagagcag caagacgttg agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc
    32461 gcattcagac acaacgcatc gcggtgggtc agagtcgtca ggttcatcat gatcctcact
    32521 tgatcgtttt tatagtcgcg ccagtgtgcc cgacaaaaca ataccattgc gtaatcaatt
    32581 tcacttgatt gaaattattt gcattttatt tgtattgttt acaggatgaa tttatcgggc
    32641 ataatggcta aattcgtatt aatgagatgc taaataatgg gacacaaacc cttataccga
    32701 cagattgctg atcgcattcg tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg
    32761 ttacctaccg aatcggcatt acaaacagag tttggcgtca gccgggttac ggtgcgtcag
    32821 gctttgcgcc agttagtcga gcaacagatc ctcgaaagca ttcagggcag cgggacttac
    32881 gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga tgaaaaactg
    32941 tcggaccgtc acgtcgatac acacagtgaa gttctgatat ttgaagtgat tccggctgac
    33001 gattttcttc agcaacagct acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc
    33061 gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg
    33121 ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat tgaagaagtg
    33181 aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc aaccgaagag
    33241 atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt atcgcgtgga
    33301 tatttagttg atggtcgggt gtttgaatat agccgcaacg cttttaatac cgatgactat
    33361 aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaagg ccatcatacg
    33421 atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga agcggcgagg
    33481 gctatttctt attacagata tttatttcag aacagttttc agtgcttcac cgatatccgc
    33541 caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct catccgcagt
    33601 ccctttccca ccggcaatga tcgcacccgc gtggcccata cgtttgcctt tcggcgcagt
    33661 cacaccagcg atgtaaccca caactggctt ggtaacatgc tctttgatgt acgctgccgc
    33721 ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg tctgcggatc
    33781 tttttcgaac atttcgagaa tgtcgataaa gttagagcca gggatcgggt caccgccgat
    33841 accgacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg cttcataggt
    33901 cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt gacccggctg
    33961 gataccaatt ttgcattcac ccggggtgat aacgcctggg cagttcgggc cgatcatacg
    34021 aacgcctgct tcgtccagtt tcactttcac ggtcagcata tccagcgtcg ggatgccttc
    34081 agtgatggtg ataatcagtt tgatgcctgc atcgatagct tccagaatgg agtctttgca
    34141 gaacggtgct ggtacgtaaa taacagacgc ggaagcgcca gtagcagcaa cagcttcacg
    34201 cacggtgttg aacaccggca ggccgaggtg ggtagtgccg cctttacctg gagttacgcc
    34261 accaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc cctggctacc
    34321 ggtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca ttatttcccc
    34381 tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc aataatattc
    34441 aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc cagacgtacc
    34501 acgaccggta cgttaacacc cacttccgct accgcgccga tgataccgtc agcgatcagg
    34561 tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt gtcgtcagag
    34621 aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc aacgtccagg
    34681 aagttagccg gttcgccgcc gtgcagttta actatgtcca tcgtacccat cgccagacct
    34741 gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag ttcccactgt
    34801 gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg cagatcaggc
    34861 tggcggaaca gggcgttgcc atcagcgccc agtttgccgt cgaggcaaat cagatcgccc
    34921 tgtttggtga tgaccagcgg gttgatttcg atcagcgcca ggtcgcgctc caggaaaatg
    34981 gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc cagacccagt
    35041 ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc aagcgcaact
    35101 ttatggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc gccttcggtg
    35161 gaggccataa agaccacgcg acgggaacta cggtctacaa cggcaccgag atacagctct
    35221 ttagcgatat cggtcgccgc ttcaaccaga atctggttaa ccggttggcc attggcatct
    35281 gtttgatacg ttaccagacg cttgcccagc cagttttctg caaaagcacg gatgtcttct
    35341 ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg aacctgacat
    35401 ttcactaccc acggaccggc accgattttt gaagcggctt cttctgcttc gcgcggagta
    35461 gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaacagttg ttttgcctga
    35521 tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta
    35581 gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta ctacacgtcc
    35641 agcagcagac gcgtcggctc ttccagtaac tctttgatcg ttaccagaaa gcccacggat
    35701 tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat cggcaggatc
    35761 tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc cagaattgcg
    35821 ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc accgttggtg
    35881 atggtgaagt taccaccggt cagatcttca accgtcagct tgccatcacg gcctttgact
    35941 gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac atcacgcaga
    36001 accggcgtca ccaggccgcg cggcgtagaa accgccatgc tgacgtcgaa atagttgtgg
    36061 taaaccacgt catcgccgtc gatagacgcg ttcacttccg ggtaacgttt cagggcttca
    36121 accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg tttttcaaac
    36181 gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc gttgaacgtg
    36241 gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg cttacgcagg
    36301 cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc cgccggagca
    36361 gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc cacatcttca
    36421 cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag attgtgttca
    36481 gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc cagagacgcc
    36541 tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt ttctttaccg
    36601 gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt tgtaccttca
    36661 tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag taccactttg
    36721 tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg ttttttatgc
    36781 caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag aatatctacg
    36841 ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc agatcttgtt
    36901 gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc gggcggcctg
    36961 cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg ctgcagtacc
    37021 atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg tgagcaaact
    37081 gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg atacgcacaa
    37141 tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa taaaccttac
    37201 cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg
    37261 ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc ggatgacgca
    37321 gcagggattt cggcgacatc acgaccagcg gacgacgcat cccgcgcagc gcctgacgac
    37381 gcagcatgtg gtaaacctgt gccggggtag acggtacgca aacctgcatg ttttgctcag
    37441 cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc tgcccttcgt
    37501 aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgc tcgccagagg
    37561 agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac tgcgcttccc
    37621 agatggtcag ggtgcgtggt tctgcggtgg cataaccata ttcaaacgcc agcacggctt
    37681 cttctgacag tacggagtcc cagacacgga acgcgccctg accgttatgg atgtgttgca
    37741 gcggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg tggaagaagg
    37801 taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcatca accagcgtgg
    37861 cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggct gccatcgcct
    37921 ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttct ggtaccgtac
    37981 tgatacgttt agccagttcc tgcaggcgct tcatctcaac tttgttcggg tactcttcgt
    38041 cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc ggacgccact
    38101 ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc atctcggtgg
    38161 catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt ttgcgcggtg
    38221 tcggatgttt tttgattttc tgatacatca gtggctgggt tgcgctcggc tcgtcggctt
    38281 cgttgtggcc gtgacggcgg tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt
    38341 tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca tccgcgttaa
    38401 cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt gtagaacggg
    38461 catccagcgg gttagaggtg gtgaaaccaa cctggttgtt gataacgata cgtaccgtac
    38521 cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc acgccctgcc
    38581 cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg ttcggctcat
    38641 caagtctgtc cagacgggca cgaacagagc cgataacgac cgggcttaca atctcaaggt
    38701 gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg aagtcagacg
    38761 agaagcccat gtggtatttc acgtcgcctg tgccgaggtg ttctttatgt ttaccggcga
    38821 actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg ttcagacgac
    38881 cacggtgcgc catcccgaga accacttcgc gggtgccgct gttgccagcg tggcggatca
    38941 tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt tttgcgccag
    39001 ggaattttgc gccgaggtaa cgctcaaggc cttcagcggc ggtcagttcg cttaagaagc
    39061 gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt tggatccagc
    39121 gtttttcttc ggtgctggtg atgtgcatat actcggcacc aatcgggccg cagtaggttt
    39181 gcttgagggc ggaaataagc tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac
    39241 cgacgttgaa ggtttcctgg aagtctgctt cggtcagatc gtggaaagac ggatccagat
    39301 cggccacttt atcttgctgc cacagtccca gcggatctag attcgcatgc tggtgaccac
    39361 ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg gtgtcagggt
    39421 cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg
    39481 tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg aacgtcgaac
    39541 gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc
    39601 agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa gcgctgttct
    39661 gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac taccacgcac
    39721 agtgatgtgc gtgccggggt tcacttgcga gctcttgtct acgtagtggc tcgcgaagga
    39781 acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc atttctttgt
    39841 tacgcctgat gcgctgcgct tatcaggcct gcgggtaatg acttgtaggc cggataaggc
    39901 gttcacgccg catccggcac tggttgcctg atgcaacgct tgcgcgtctt atcaggccta
    39961 cagtttacgc attacgttgc aacaacatcg acttgatatg gccgatggcg cgcgtcgggt
    40021 tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg aatacgctga
    40081 atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta tcgatcagga
    40141 aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc caccagaaag
    40201 acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg tcgagttttt
    40261 cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca ttattcaaca
    40321 ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc accaaatcgc
    40381 ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc tggttgagtg
    40441 ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga ccgtcggaac
    40501 cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc tctttcagct
    40561 ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct
    40621 gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac tcgagtctca
    40681 ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg
    40741 gttccatgtt gacgcttcgg cgcgtcatgg attccgactc aggcagatac agggagtggc
    40801 acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg ccacggcttt
    40861 cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg ttatccagtt
    40921 ccaggcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga cgggcatttt
    40981 tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca tcaccttcac
    41041 ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacggatc gccaccggat
    41101 cttcaccgtt acgattattg ttccaccggt tcaggcgatc cagagacgct tcaacatcag
    41161 actcgctggc atcgtgcagt gcgccctgct cggcgataga ctcttgcaga tgcagacctg
    41221 ccgcgcgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg ttagcgccgt
    41281 gtaccgatac acaagcgatt tcaccaacgg caaacagtcc cggaacaacc acatcttcgc
    41341 ctttctcatt cacagtcagc gcctgaccgg taactttggt cggaataccg cccatcatgt
    41401 agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga gcgaaggtac
    41461 gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc aggtgatcga
    41521 gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca cggatttcaa
    41581 tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc ggcgcataac
    41641 gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca cggcaacctt
    41701 cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac atttccatat
    41761 cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg ttaatgtggg
    41821 cgttagtggt ggactgataa atacgccctg ctccgccagt cgccagcacg gtagcgcggg
    41881 ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc accaccgcgc
    41941 catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg gtgtggtttt
    42001 tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca gcagccgccg
    42061 cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac ggacgttgat
    42121 agatacgacc atcatcgaga cgcgagaacg gcaggcccat gtgttcgagt tccagaatcg
    42181 cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata tagtccgacc
    42241 cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta cccagcgcaa
    42301 cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact ttagagagca
    42361 gggcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct gcgccaccgg
    42421 caccaatcac aactgcatca aattctctga ctggcaattt catcacacac cccacaccac
    42481 aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt gcagcatcag
    42541 gcgcaaggcc agcggtttaa cgtagtcggt caacacctgc cacatgccga tccaggcatg
    42601 aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag aggcgaagaa
    42661 accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga ccatataaat
    42721 gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat gtacgccatt
    42781 gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag tgaaagcacg
    42841 acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt ttcttccaga
    42901 tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc cagagcggta
    42961 aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga agcttgctcg
    43021 aaaccttcag gggaagagag gctggtaccc agaagccact gcaggatgcc cactgcaaca
    43081 aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat ggggaaccgg
    43141 atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat catgaataac
    43201 gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta actttctgtt
    43261 acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc
    43321 cataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg ttacaacgct
    43381 gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc tgttcacaaa
    43441 atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt gatccaggtc
    43501 acgataacaa catttattta atttttaatc atctaatttg acaatcattc aacaaagttg
    43561 ttacaaacat taccaggaaa agcatataat gcgtaaaagt tatgaagtcg gtatttcacc
    43621 taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga cagttattag
    43681 tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt attcgtcatc
    43741 aatggattct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc ctctttcaca
    43801 gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg caaatttaag
    43861 ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat acaaaagcaa
    43921 aactcaacct caacggggac acagctgttg aactggatgt gctgaaaggc acgctgggtc
    43981 aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt gacccaggct
    44041 tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat gaaggtattt
    44101 tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac ctagaagttt
    44161 gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta
    44221 cggtgacccg tcataccatg atccacgagc agattacccg tctgttccac gctttccgtc
    44281 gcgactcaca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc
    44341 acgactcgct ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt
    44401 cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag ccatttgttt
    44461 atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc tccacgccgt
    44521 gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc
    44581 acgctgacca tgaacagaac gcctctacct ccaccgtgtg taccgctggc tcttcgggtg
    44641 cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct gcgcacggtg
    44701 gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa cacattccgg
    44761 aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc ttcggtcacc
    44821 gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc
    44881 tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg gaaaacatcg
    44941 cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat ttctactctg
    45001 gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt ttcgcaatgg
    45061 cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagttacggt atgaagattg
    45121 cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc gatatcaagc
    45181 gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg gcgggttaat
    45241 ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc cactatcgga cattcatata
    45301 ttctaaaaaa aaatacatcg atataaatta taaacaaata gatgattttt aatcatctat
    45361 cttcctctca gttacatgaa taaaccacca aatatagcgc acccctataa ttcccaatac
    45421 tttattcatc aaacatttaa ttcataccat caattaatta cttttacatt taataaaatt
    45481 aataatattt ttaagaaaaa ctaaaaacat tcgacaatca aataagatat tcttgcaccg
    45541 atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa aaaacattgg
    45601 ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg
    45661 gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata
    45721 ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca
    45781 gcgatgccgt tgctgtcgac ctgctcttag tcaactgtga tctggaaaac tccagcaacg
    45841 gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg
    45901 gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt
    45961 taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg
    46021 ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg
    46081 ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat
    46141 aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac
    46201 cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt
    46261 ggcggtcgaa ttcaatctga atgttctcga taaatcaatg cgcgaccgca ttgatatttc
    46321 attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa
    46381 taataaccaa atcagtaacg agcaaaagat taactggcac aaaaatgacg ataaaaccat
    46441 tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc
    46501 gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt
    46561 cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg cgtggcactc
    46621 agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta
    46681 caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc
    46741 ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa
    46801 tcatactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt
    46861 tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagtcccaa
    46921 tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc
    46981 atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt
    47041 gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat
    47101 cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga
    47161 tggtcgggtg aacaatttcc aggtttcagc agcatcgacg cccttcctga ctcgtcaggg
    47221 acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga
    47281 aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta
    47341 cagcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa
    47401 tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc attttgatac
    47461 tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac
    47521 taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc
    47581 caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag
    47641 cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca
    47701 tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt
    47761 tgatattggt gactggagag atatctctat ttctacgtca ttcaatacaa cccattacga
    47821 agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg
    47881 tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa
    47941 cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc
    48001 tgacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga
    48061 tatttctggt acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc
    48121 tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg
    48181 cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac
    48241 caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc
    48301 ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac
    48361 gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa
    48421 cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga
    48481 tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt
    48541 cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct
    48601 tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa
    48661 atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc
    48721 cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc
    48781 agtctgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa
    48841 aatgaaaaag gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg
    48901 gaaccgaaag caacaacaca ggtacggata gtaaagcaag cctcgaccac aaaattaccg
    48961 ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa
    49021 aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga
    49081 ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag
    49141 cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg
    49201 ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt
    49261 acggtcaaca caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat
    49321 gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc
    49381 gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc
    49441 tgtcgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag
    49501 aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga
    49561 tctgggaatc tgacgatatt aaaataagat ctgggaatct gacgatatta aaattcccgt
    49621 ctattgtgac aacaatacca acggcaactt tgaaagcgaa catgtctacg catgggtaaa
    49681 cccctacccg ggggtgcagg atcgttatta tcaactcgga gtaacttaca atggcgttga
    49741 ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtatcg acagtaagaa
    49801 cattgacatt tatacccctg atgggatggc agaacaaaat ttgctcgggt gatcccgcta
    49861 atattcacat gtcgcgaacc tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa
    49921 tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa tttgacggtg
    49981 ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt actggtctgg
    50041 aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa acacagatga
    50101 ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg aaaacattca
    50161 gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa gtcagttcct
    50221 ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc
    50281 tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat
    50341 acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct gaactttcgt
    50401 ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt tttaaaatta
    50461 actataactg atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat
    50521 tgctgatttt ccccttttcc ttgattgctg ttattttttc ctagcccggc tttctttatg
    50581 ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg
    50641 ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg
    50701 gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact
    50761 ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg
    50821 caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct
    50881 attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc
    50941 tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc
    51001 gctatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca atatctgcga
    51061 gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg tgataacgct
    51121 gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg
    51181 attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg
    51241 atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa
    51301 ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt
    51361 ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt
    51421 tttgctctgc tggctatttg tgcaggtatg gcgtggggac tgacccggtt tatgcatatt
    51481 gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc
    51541 gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc
    51601 agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc gccgaagcgg
    51661 tcggcctagt gctggcagcc tggacaccag taaaacgggc gcgaggacaa cgtggttttc
    51721 tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga
    51781 aacagcgccc catgatgctt attctcatcc acctgccccc gcgtagcgta agaaaaccga
    51841 ggaatctcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgct
    51901 ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc
    51961 ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggc
    52021 gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca
    52081 acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag
    52141 gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg
    52201 gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg
    52261 tagagcgtta aaccgttggg aaaatgagtt aacaacgcct taccacgcgt ttccacatgg
    52321 gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag
    52381 acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc
    52441 tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg ggttatgctg
    52501 cgacaacaat cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt
    52561 gctcgccgtc gccatgcagg cagaccgttt gcgccgcgac cgttgcccat tcgccggtga
    52621 tacttttgac tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt
    52681 tttcaatcaa cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc
    52741 gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct
    52801 cgcttcctgc cagcccaacg agaaccaatg ccggatcgca agcgtatacc gctctggcga
    52861 tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga
    52921 catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca
    52981 gcgtctgggc gtaaacggtt tccggcggca gctgcatggc gctgcgacca aaattttccc
    53041 tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc
    53101 aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca agcaatattg gcagaggaaa
    53161 ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa
    53221 ttttcattgt gcagccgcca cgctaattgt tcaaaataac gttgctgatc ttgccgcgct
    53281 ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc
    53341 gccagatggt acatatcagc ctcaatgata caggcaatac gtggataacc accggtggtc
    53401 tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg gcacctgcac cacgcccggt
    53461 aacaaaccgt gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa
    53521 cgatagccca ttcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc
    53581 tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc
    53641 cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg
    53701 agtcagtcac catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg
    53761 gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg
    53821 cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc
    53881 cagccggtcc agacggcgtt atcatccaac cgcgcttcgc aacctgcacc cgtcagggca
    53941 aaccacccat cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg
    54001 gcattggcgt cattacccac cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg
    54061 ccacagtggc tgatacccga ctggcaaaaa ccgtgacgac cgccatcctg cacagtggtg
    54121 tacatgcccg cacgaataat cttcagcata ttccctcctt ctgcggtaca aatcgcacgc
    54181 tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg
    54241 tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc
    54301 cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt
    54361 gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga
    54421 ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg
    54481 ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa
    54541 agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg
    54601 catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa
    54661 tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag atccgtttct
    54721 ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat
    54781 aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata
    54841 tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata
    54901 ccaccgcgtt cagtggcatg gtgacctgca gcataaaaat gcaatccctg ctcgcgggct
    54961 gaatgaatgg tctgctcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg
    55021 gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca
    55081 ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct
    55141 agttccattc ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg
    55201 cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt
    55261 gcgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa
    55321 cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc
    55381 acaatgaccg catctgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg
    55441 acaccggtaa caattttttg caccgtctct ttgccttcca cctgcaaacc attcggcgcg
    55501 taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt
    55561 ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt tacatcgcct
    55621 gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa
    55681 attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca
    55741 taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac
    55801 tattacgtga cgttgccgtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc
    55861 gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc
    55921 gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg
    55981 agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg
    56041 cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg
    56101 gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga
    56161 ttatcgtctg cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc ggcgagctgt
    56221 atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg
    56281 tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga
    56341 tgggctttgg cctggcggcg gttggcatga tcgcgggtct ggtcattttc ttatgtggca
    56401 atcgtcattt cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc
    56461 tcctgccgaa ctggggatgg ctgctggttc tgctggtggc aacgcctgca ctgattaccg
    56521 tgctgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg
    56581 gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcggaag gagctggggc
    56641 tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa cagggcggta
    56701 gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc
    56761 cgaccgcgat gttccagtcg attaatgcct tcgcagttat gctgtgcggt gtgttcctgg
    56821 cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg
    56881 ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt
    56941 ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct
    57001 ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg
    57061 gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc
    57121 tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca
    57181 actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt
    57241 gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc
    57301 tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat
    57361 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggattaggc
    57421 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
    57481 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
    57541 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt
    57601 tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc tcacggtcaa
    57661 atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca tcagtacccg
    57721 tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg
    57781 acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg
    57841 ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
    57901 catcaataat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc
    57961 tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac aacgaggggt
    58021 aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac
    58081 cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag
    58141 gcgataaccc gcccgttgcc aagctggcgg acaaacggct ggtgccttcc actgccggaa
    58201 aatctcgttg ttgctcatac tctccagcac cgttctcgca aaactggcgt aattgcgcaa
    58261 tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca
    58321 gcgtaatgga tggcgcgggc tttatcgatc cgctactacg aacttttggt gcagcgacgc
    58381 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
    58441 tgtacatctc gtgattgccg gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa
    58501 atccttcaca caccacgtta cgcagcgctc tttcaacgtc cacatcccgc gctcgctcat
    58561 tcacctcata ctgatagtta taaaaaagat gagtaacgct gttttccgcg cacacctgtt
    58621 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
    58681 tttccgccag cgcgatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
    58741 gcgacatgtt atgcgtctcc cactggcgtg gtgtagcgat atacagcgcc agcacgcgtg
    58801 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc
    58861 gaaaccaggc cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
    58921 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg
    58981 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
    59041 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
    59101 catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg
    59161 ttggcggtac tggttgtaaa aatcatccag attttgccac tgatgaatat cagccacaaa
    59221 cgggccaagt ttgcggtact cgggctgaga atgggccaga aagacgagtt tatagcggct
    59281 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
    59341 aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
    59401 tgaacggtat acaggagtaa gtgtaggcgt tggtaaatga tgtttgtgaa gttaatccgc
    59461 tagcagggca agattacgaa agtgcgctcc ccgcaagcaa ctaatcaagg tagttaatca
    59521 tggctgaaca cgatattacg ccagaagtca ccatttccaa aacacagcgc cgctataaag
    59581 tcggctatgt cagagcccgc caccaggacc gcaaaaccgg tatagcccgg tattattcac
    59641 aacatcccag cctgcacctt aagggcgact ggctgaaaga ggccggattt gataccggac
    59701 ggggtgtcac ggtgaagatt tcggaggggg gcctgaccat catcgctgac agtgacgagg
    59761 tgcaggaact gcgggatggg atgtttagtg tgctgaacga aagttaaata ttaaaaaagc
    59821 tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg atactcatca
    59881 tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa cctagttttt
    59941 aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga tggatcgtat
    60001 tcattatcta tgtcagaagc taaaaatgat gatattcgta gtttaaattt agcttcatct
    60061 ttattacatt cattggcata aggtgaatgc cctatcatag catattcatc taatctaaaa
    60121 ggataagatg gttttaattc atcgatcaat gaaatttcaa tccatgttct atatccaggc
    60181 attaatggac taatttcttt gagcaaaacc tttccactta ataatgtatt gagattgttc
    60241 attattatgt ctcccacggg aaaaatatat gtctaacact tccaccatgt tgatgaactt
    60301 gtaaatgtgg taaatcacca tcaggatttc ctggtccatg acctgacact ctatatgaag
    60361 gttttgttac agaaggatct tttatatcat cccaatgata tgaacctgct tttgttttat
    60421 ttccagtaag atattccatt aagttcttag tgctggggcc tgactcacct gtcatatttt
    60481 tataattatg acctaaatat ctatccataa acaattgttc agcgtcactt ctagacctaa
    60541 cagtaacttt ggtttcacta ctatctaata aacgattcgc tttttggtta ctaacagcac
    60601 atggagttaa tccaagtgga tcagcatatt ttagaggatt aggtgcataa ctgtagagat
    60661 ttaaaccgcc tctcaaccct atcgggtcag ttaccatgaa ccgcccgaca tccggatcat
    60721 agtacctgtg cagattgtag tgcagccccg tttccctgtc gaggtactgt ccctggtagc
    60781 gcaggttctg ctccaccccg ccgtggtcct tctcctgtat cgtgttaccc cagacctgat
    60841 accccgtctc ccacacgatt ttcccgtcgc tgtccgtcat ctcttccggc gcgccgttca
    60901 catccgtgtg aaaatacagt atccggttcg ggccctcttt gcccgcctta tccacccgcg
    60961 ccagcggttc ataactcccc tggtccctgt agatgtacag gctgcctgtt ccctccggcc
    61021 tgctctcctg cgccagccgc attccgtccc ataaaaattt tgtccggttg tacggcttac
    61081 cgtcagggct gatgtgctgt ttttccgtcc gcctccccag tgcatcgtac acataccggt
    61141 atgtcccccg cgctgtcgtc acttccacca tccggtgttc cgcatcataa cggtaactct
    61201 gcgttccgct ccggccctcc ttttccaccg cacggccaaa ttcgtcataa cggtagtgca
    61261 caccacggta agacatcagc cggttgaacg gcaccacgct gttttctgcc gtggcccttt
    61321 cccccgcgcg cctccccagc aggtttcccg ccgcgtcgta ctgccagctc gccagatacc
    61381 cctcatcctc cgaacgacac gccgtgatgc gccccgtggg gtcgtagaag taatccgttt
    61441 ccccccgccg gctgtgccgc gtcttcagca gttcatcctc gccactgtag ctgtactgcc
    61501 ggtccgtgaa cgtctccggt accaccccgc gcatcccgct gtatgtctcc ctgcgcttca
    61561 gccgcccgcc ggggtcgtaa agctgccgcg tgctcagtcg cccctggctt ctgagcactt
    61621 cacggtgcag gtggtcgcgc gtgaactcgc tgatgacctc ccggtccaga tttatctgct
    61681 ggagatggcc gctgccgtag tacagatggc gctgcgttct gccgtccgga aaggtggtgg
    61741 cagtccggtt gcccagttta tcgtactggt gggtcaggat gccctgcgcc gtctcctcgc
    61801 tcagcatccg ccccagtctg tcgtagctga agcggataac ttccggcgcg tcagtttcgt
    61861 gccgctggcg acggatttcc agtagctggt ctgccgcatc atggacgtat tccgtacggc
    61921 catcggctgt ctcttttgcc gtgagccggc ccgccgcatc acgcgccagc cggtggcgga
    61981 tttcaccgcc ccggtccccg gtgaacacca cttccgtgac cgcgttcagc gcgttgtagc
    62041 ggtacgactg gcggcagccg tccgggttta tctgctccgc cagacggtcc agcacatcat
    62101 ggcggaaccg gtagcgttct ccgttctcgt tcgtgagcgt gaccagacgc ccgtaagcgt
    62161 cgtacccgaa tgccgtcctg cgccccatcg cgtccgtccg gctgcgcacc cggccctgcc
    62221 cgtcccgctg ccagcgcgtt atcctcccca tcgggctggt gtactttacc agcctgcccg
    62281 ccgcgtctga ctggtaattc accgtgcggc cgtccgccag tatcacctcc gtcagatgac
    62341 cgctgtcgtc atacccgtag cgcgtgctgt ggctctgcgc atccgtcatt ctgataagcc
    62401 tcgtgcgttc gtcgtagaac cacgccgtct gcgagccgga acagtccgtg tgccgcatga
    62461 gctgcccgtc ttcattccac tggaggtatt tattcccccc gcgcgcatcc gttatctgca
    62521 ccacctggcc gtggtggtca tactgatacc gggtgttctg ctgcagcggg tcggtcacgg
    62581 caagaaggtt gccgcgttca tcgtacacac agcgccaggt gtttcccgcc gcgtccacct
    62641 ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc cccagcgggt
    62701 cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg ccgccctgag
    62761 ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc
    62821 cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga taacgtgtgc
    62881 gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc agccggtact
    62941 gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat
    63001 accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt
    63061 ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac acgccgttca
    63121 ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat
    63181 gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac agacggatgc
    63241 acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg tagtacagat
    63301 tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg
    63361 ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag tgcccctgct
    63421 caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg
    63481 cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt tcgtctgcgc
    63541 tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga ccaaacattc
    63601 cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg
    63661 gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg gcggcattga
    63721 ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg acactggcag
    63781 caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc gccacggttg
    63841 ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt aacggaccgc
    63901 caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc accctggcct
    63961 cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg
    64021 cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac agattactga
    64081 tgctgccaat aaccgcattc actccctgaa agacggtttc cagcataccg cccggattca
    64141 gcaggttaag ggccgttccc aggggggcat ccagaaaggc ggcttttttt tgtgcgagaa
    64201 actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg
    64261 atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa tgtgtcacgc
    64321 catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga gcccgggaca
    64381 tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc atttccagat
    64441 attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct tcttcctgtt
    64501 ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg
    64561 cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg atgtaacctt
    64621 caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg accttttctg
    64681 tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg acggccgtgc
    64741 accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat
    64801 caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc ataaacgacg
    64861 tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg tcccgttccc
    64921 tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc tttttaattt
    64981 cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag gtgaaggcca
    65041 tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc tgctcgttac
    65101 cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt ccgtcataat
    65161 gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt tttaatcatg
    65221 aatgtaatac ccatcaccgc tttacagcac actttatctt tccagccata atcactaata
    65281 atattaagca attagcaata tttacaacga ccagagcgga tacatagtta tgtatttaac
    65341 aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct accgcctcca
    65401 tataaaaatg gacaccttat aaaattgtat cttgcataga aaaagctgaa atatcggatg
    65461 cgttttaatt atcaactata aatctataag ctttttatta acaacaa
//
LOCUS       DAECOD010000009        61933 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.009, whole genome shotgun sequence.
ACCESSION   DAECOD010000009 DAECOD010000000
VERSION     DAECOD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.009"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            425..511
                     /gene="leuL"
                     /locus_tag="KKO15_000568"
     CDS             425..511
                     /gene="leuL"
                     /locus_tag="KKO15_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBD6359486.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            604..2175
                     /gene="leuA"
                     /locus_tag="KKO15_000569"
     CDS             604..2175
                     /gene="leuA"
                     /locus_tag="KKO15_000569"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBD6359487.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2175..3266
                     /gene="leuB"
                     /locus_tag="KKO15_000570"
     CDS             2175..3266
                     /gene="leuB"
                     /locus_tag="KKO15_000570"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBD6359488.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3269..4669
                     /gene="leuC"
                     /locus_tag="KKO15_000571"
     CDS             3269..4669
                     /gene="leuC"
                     /locus_tag="KKO15_000571"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBD6359489.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4680..5285
                     /gene="leuD"
                     /locus_tag="KKO15_000572"
     CDS             4680..5285
                     /gene="leuD"
                     /locus_tag="KKO15_000572"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBD6359490.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5334..6308)
                     /locus_tag="KKO15_000573"
     CDS             complement(5334..6308)
                     /locus_tag="KKO15_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359491.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALARVELPSENALSMQGDWQDSNVRMLFVASTLMW
                     TCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVI
                     AVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLF
                     TNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV"
     gene            complement(6410..6541)
                     /gene="sgrT"
                     /locus_tag="KKO15_000574"
     CDS             complement(6410..6541)
                     /gene="sgrT"
                     /locus_tag="KKO15_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBD6359492.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6630..8285
                     /gene="sgrR"
                     /locus_tag="KKO15_000575"
     CDS             6630..8285
                     /gene="sgrR"
                     /locus_tag="KKO15_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBD6359493.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8319..8418
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8449..9432
                     /gene="thiB"
                     /locus_tag="KKO15_000576"
     CDS             8449..9432
                     /gene="thiB"
                     /locus_tag="KKO15_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBD6359494.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9408..11018
                     /gene="thiP"
                     /locus_tag="KKO15_000577"
     CDS             9408..11018
                     /gene="thiP"
                     /locus_tag="KKO15_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBD6359495.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11002..11700
                     /gene="thiQ"
                     /locus_tag="KKO15_000578"
     CDS             11002..11700
                     /gene="thiQ"
                     /locus_tag="KKO15_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBD6359496.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11814..12578)
                     /locus_tag="KKO15_000579"
     CDS             complement(11814..12578)
                     /locus_tag="KKO15_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6359497.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12664..13542)
                     /gene="araC"
                     /locus_tag="KKO15_000580"
     CDS             complement(12664..13542)
                     /gene="araC"
                     /locus_tag="KKO15_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBD6359498.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13881..15581
                     /gene="araB"
                     /locus_tag="KKO15_000581"
     CDS             13881..15581
                     /gene="araB"
                     /locus_tag="KKO15_000581"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBD6359499.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSMVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15592..17094
                     /gene="araA"
                     /locus_tag="KKO15_000582"
     CDS             15592..17094
                     /gene="araA"
                     /locus_tag="KKO15_000582"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBD6359500.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17294..17989
                     /gene="araD"
                     /locus_tag="KKO15_000583"
     CDS             17294..17989
                     /gene="araD"
                     /locus_tag="KKO15_000583"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD6359501.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18064..20415
                     /gene="polB"
                     /locus_tag="KKO15_000584"
     CDS             18064..20415
                     /gene="polB"
                     /locus_tag="KKO15_000584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBD6359502.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20580..23486
                     /gene="rapA"
                     /locus_tag="KKO15_000585"
     CDS             20580..23486
                     /gene="rapA"
                     /locus_tag="KKO15_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBD6359503.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23498..24157
                     /gene="rluA"
                     /locus_tag="KKO15_000586"
     CDS             23498..24157
                     /gene="rluA"
                     /locus_tag="KKO15_000586"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBD6359504.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24274..25089)
                     /gene="djlA"
                     /locus_tag="KKO15_000587"
     CDS             complement(24274..25089)
                     /gene="djlA"
                     /locus_tag="KKO15_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBD6359505.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25344..27698
                     /gene="lptD"
                     /locus_tag="KKO15_000588"
     CDS             25344..27698
                     /gene="lptD"
                     /locus_tag="KKO15_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBD6359506.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27751..29037
                     /gene="surA"
                     /locus_tag="KKO15_000589"
     CDS             27751..29037
                     /gene="surA"
                     /locus_tag="KKO15_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBD6359507.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29037..30026
                     /gene="pdxA"
                     /locus_tag="KKO15_000590"
     CDS             29037..30026
                     /gene="pdxA"
                     /locus_tag="KKO15_000590"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBD6359508.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30023..30844
                     /gene="rsmA"
                     /locus_tag="KKO15_000591"
     CDS             30023..30844
                     /gene="rsmA"
                     /locus_tag="KKO15_000591"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBD6359509.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30847..31224
                     /gene="apaG"
                     /locus_tag="KKO15_000592"
     CDS             30847..31224
                     /gene="apaG"
                     /locus_tag="KKO15_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBD6359510.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31231..32073
                     /gene="apaH"
                     /locus_tag="KKO15_000593"
     CDS             31231..32073
                     /gene="apaH"
                     /locus_tag="KKO15_000593"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBD6359511.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32151..32630)
                     /gene="folA"
                     /locus_tag="KKO15_000594"
     CDS             complement(32151..32630)
                     /gene="folA"
                     /locus_tag="KKO15_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBD6359512.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32821..34683)
                     /gene="kefC"
                     /locus_tag="KKO15_000595"
     CDS             complement(32821..34683)
                     /gene="kefC"
                     /locus_tag="KKO15_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBD6359513.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34676..35206)
                     /gene="kefF"
                     /locus_tag="KKO15_000596"
     CDS             complement(34676..35206)
                     /gene="kefF"
                     /locus_tag="KKO15_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBD6359514.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35254..36648)
                     /locus_tag="KKO15_000597"
     CDS             complement(35254..36648)
                     /locus_tag="KKO15_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359515.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36707..36994)
                     /gene="fixX"
                     /locus_tag="KKO15_000598"
     CDS             complement(36707..36994)
                     /gene="fixX"
                     /locus_tag="KKO15_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBD6359516.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(36991..38277)
                     /locus_tag="KKO15_000599"
     CDS             complement(36991..38277)
                     /locus_tag="KKO15_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6359517.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38328..39269)
                     /locus_tag="KKO15_000600"
                     /pseudo
     CDS             complement(38328..39269)
                     /locus_tag="KKO15_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39284..40054)
                     /locus_tag="KKO15_000601"
     CDS             complement(39284..40054)
                     /locus_tag="KKO15_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBD6359518.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40526..42040
                     /gene="caiT"
                     /locus_tag="KKO15_000602"
     CDS             40526..42040
                     /gene="caiT"
                     /locus_tag="KKO15_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBD6359519.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42071..43213
                     /gene="caiA"
                     /locus_tag="KKO15_000603"
     CDS             42071..43213
                     /gene="caiA"
                     /locus_tag="KKO15_000603"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBD6359520.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43342..44559
                     /gene="caiB"
                     /locus_tag="KKO15_000604"
     CDS             43342..44559
                     /gene="caiB"
                     /locus_tag="KKO15_000604"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBD6359521.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44633..46186
                     /gene="caiC"
                     /locus_tag="KKO15_000605"
     CDS             44633..46186
                     /gene="caiC"
                     /locus_tag="KKO15_000605"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBD6359522.1"
                     /translation="MDIIGGQHLRQIWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46295..47080
                     /gene="caiD"
                     /locus_tag="KKO15_000606"
     CDS             46295..47080
                     /gene="caiD"
                     /locus_tag="KKO15_000606"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBD6359523.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47086..47676
                     /gene="caiE"
                     /locus_tag="KKO15_000607"
     CDS             47086..47676
                     /gene="caiE"
                     /locus_tag="KKO15_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBD6359524.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47762..48157)
                     /gene="caiF"
                     /locus_tag="KKO15_000608"
     CDS             complement(47762..48157)
                     /gene="caiF"
                     /locus_tag="KKO15_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBD6359525.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48419..51640)
                     /gene="carB"
                     /locus_tag="KKO15_000609"
     CDS             complement(48419..51640)
                     /gene="carB"
                     /locus_tag="KKO15_000609"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBD6359526.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLALSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51658..52806)
                     /gene="carA"
                     /locus_tag="KKO15_000610"
     CDS             complement(51658..52806)
                     /gene="carA"
                     /locus_tag="KKO15_000610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBD6359527.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(53262..54083)
                     /gene="dapB"
                     /locus_tag="KKO15_000611"
     CDS             complement(53262..54083)
                     /gene="dapB"
                     /locus_tag="KKO15_000611"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBD6359528.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(54250..55164)
                     /gene="rihC"
                     /locus_tag="KKO15_000612"
     CDS             complement(54250..55164)
                     /gene="rihC"
                     /locus_tag="KKO15_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBD6359529.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(55230..56180)
                     /gene="ispH"
                     /locus_tag="KKO15_000613"
                     /gene_synonym="lytB"
     CDS             complement(55230..56180)
                     /gene="ispH"
                     /locus_tag="KKO15_000613"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBD6359530.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56182..56631)
                     /gene="fkpB"
                     /locus_tag="KKO15_000614"
     CDS             complement(56182..56631)
                     /gene="fkpB"
                     /locus_tag="KKO15_000614"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6359531.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56718..57212)
                     /gene="lspA"
                     /locus_tag="KKO15_000615"
     CDS             complement(56718..57212)
                     /gene="lspA"
                     /locus_tag="KKO15_000615"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBD6359532.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57212..60028)
                     /gene="ileS"
                     /locus_tag="KKO15_000616"
     CDS             complement(57212..60028)
                     /gene="ileS"
                     /locus_tag="KKO15_000616"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBD6359533.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60071..61012)
                     /gene="ribF"
                     /locus_tag="KKO15_000617"
     CDS             complement(60071..61012)
                     /gene="ribF"
                     /locus_tag="KKO15_000617"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBD6359534.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61020..61238)
                     /locus_tag="KKO15_000618"
     CDS             complement(61020..61238)
                     /locus_tag="KKO15_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBD6359535.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61341..61604
                     /gene="rpsT"
                     /locus_tag="KKO15_000619"
     CDS             61341..61604
                     /gene="rpsT"
                     /locus_tag="KKO15_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBD6359536.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14909 a        16891 c        16092 g        14041 t
ORIGIN      
        1 ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc ttatttaata attaatttat
       61 atatttaatg cattaattct taacattaat tgatcaataa tattcaccaa atcaatatca
      121 aaaaaaatcg caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg
      181 cattttgtcc cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac
      241 cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgtagca
      301 caattagcta attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg
      361 gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga
      421 tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg
      481 cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc
      541 gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa
      601 accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta
      661 caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt
      721 gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa
      781 accatcgccc gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa
      841 gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt
      901 attgctacct ccccaatgca catcgccacc aagctgcgca gcaccctaga cgaggtgatc
      961 gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct
     1021 tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt
     1081 aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag
     1141 ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt
     1201 tccgtacata cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat
     1261 gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt
     1321 tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc
     1381 gccattaatc accaagagat atggcgcacc agccagttag ttagccagat ttgtaatatg
     1441 ccgatcccgg caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc
     1501 caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt
     1561 ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat
     1621 cgcatggatg agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct
     1681 ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc
     1741 ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag
     1801 tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa
     1861 gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc
     1921 actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat
     1981 gcgctgggtc aggtggatat tgtcaccaac tacaacggtc gccgcttcca cggcgtcggc
     2041 ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc
     2101 tggcgtgccg cagaagtcga aaaagagttg caacgcaaaa ctcaacacaa cgaaaacaac
     2161 aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccggga gacggtattg
     2221 gtccggaagt gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac cgctttgcga
     2281 tgcgcatcac caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac
     2341 cactgccgcc tgcaacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg
     2401 taggcggccc gaagtgggaa catttaccac cagaccagca accagaacgc ggcgcgctgc
     2461 tgccgctgcg taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg
     2521 ggctggaagc attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatccagt
     2581 gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg
     2641 gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg
     2701 cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag
     2761 ctaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat
     2821 acccggatgt cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag
     2881 atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg
     2941 agtgcgcaat gatcactggc tcgatgggca tgttgccttc cgccagcctg aacgagcaag
     3001 gttttggact gtatgaaccg gcgggcggct cggcaccaga tatcgcaggc aaaaacatcg
     3061 ccaacccgat tgcacaaatc ctgtcgctgg cactgctgct gcgttacagc ctggatgccg
     3121 atgatgcggc ttccgccatt gaacgcgcca ttaaccgtgc attagaagaa ggcattcgca
     3181 ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg
     3241 cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga
     3301 cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct
     3361 ggtgcatgaa gtgacctcac cgcaggcgtt cgatggcctg cgcgcccacg gtcgcccggt
     3421 acgtcagccg ggcaaaacct tcgccactat ggatcacaac gtctctaccc agaccaaaga
     3481 cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa
     3541 agaatttggc gtcgagctgt atgacctgaa tcacccgtat caggggatcg tccacgtaat
     3601 ggggccggaa cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac
     3661 cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca
     3721 cgtactggca acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca
     3781 gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac
     3841 tggcagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt
     3901 aagcatggaa ggtcgtatga ccctgtgcaa tatggcgatc gaaatgggcg caaaagccgg
     3961 tctggttgct cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa
     4021 aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc
     4081 aactttcgat accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg
     4141 caccaatccc ggccaggtga tttctgtgaa cgacaatatt cccgatccgg cttcgtttgc
     4201 cgatccggtt gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg
     4261 tattccgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg
     4321 cattgaagat ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt
     4381 gcaggcactg gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct
     4441 ggataaaata tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct
     4501 ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa
     4561 ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc
     4621 cgctgctgct gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca
     4681 tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg
     4741 ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg
     4801 cgcatctgtt taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact
     4861 tcgtgctgaa cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg
     4921 gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg
     4981 tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc
     5041 cggtgaaatt aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg
     5101 ggatccattt cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc
     5161 gctttaccat cgatgccttc cgccgccact gcatgatgaa tggtctggac agcatcggcc
     5221 tgaccctgca gcacgacgac gctattgcgt cttatgaagc aaaacaacct gcgtttatga
     5281 attagacccc ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg
     5341 tctttaacct ttgcggttaa aaataatgcg acaacagaaa taatcgcaat tacccagtag
     5401 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacaccagc cagaattacc
     5461 ccggtagaga tagtgttggt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
     5521 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
     5581 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccagtgta aaacagcact
     5641 cctgccacca ctgcaatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
     5701 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
     5761 ttgtctggca atcctaaatc gctactgatc cacaatggca tatcaataat gtacatagtg
     5821 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
     5881 tcaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccag agcaaataac
     5941 tgtggcattg ccgtattggc cagagatgcc agaagcacac cacaggtgat aagcgtcaga
     6001 taatgacgat taaatgcaaa cagtagcgca ttgccaatag ccatcaaaca gcaaaatata
     6061 atcagttttc gccgatcgcc ctgactgtca gaacgttttg ccaaccagag gcttacgccg
     6121 atcccagcaa tagcattcac cgtataaaag agtccgatcc agaaaggttg cgcgccaacc
     6181 tcacgactca gaaataagct caatgtagga gcctgtagcg ccccggccac ccccatcata
     6241 aaagcgacca gcataaatgc cgcgtaaaca ccgttcatac ggcgagccat cgtcattatc
     6301 cagatcatac gttccctttt tagcgcggcg agaataaaaa aaaccagcag gtataatctg
     6361 ctggcgggtg attttacacc aatactcagt cacacatgat gcaggcaagt caactttcag
     6421 aattgcggtc atcccactgc atcagtcctt caagcatggt taatcgttgt gggacgctta
     6481 accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa aactgacgca
     6541 tggggcacct ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt
     6601 gctgtctttt gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca
     6661 tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag
     6721 cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg caggatcgcg
     6781 gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct
     6841 ataccgggct ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg
     6901 accaactagt gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc
     6961 tgggccgcag cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc
     7021 gtaatctgct acctggcagc gcattgcgcc gttccgaaac ccatattgcc cggcaaatct
     7081 tcagttcgct aacgcgcata aatgaggaaa atggggaact ggaagcagac atcgcccacc
     7141 actggcagca aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc
     7201 atcatggtcg tgaactggaa atggacgacg tgatcgcctc tttaaaacga atcaatacgc
     7261 tgccgctcta ttcgcatatt acggacattg tgtcgccgac gccctggacg ctggatatcc
     7321 atctcacgca gccggaccgc tggttgccgt tactgctggg gcaagttccg gcgatgatcc
     7381 tgccgtgcga atgggaaacc ctcagtaact ttgccagcca tcctattggc accggtccgt
     7441 atgcggtgat tcgcaacagc accaatcaac tgaaaattca ggcattcgat gacttcttcg
     7501 gttaccgggc attaatcgac gaagttaacg tctgggttct gccggaaatt gccgacgagc
     7561 cagccggtgg tctgatgcta aaaggtccac agggcgagaa aaaagagatt gaaagccgcc
     7621 tggaggaagg ttgctactat ttactgttcg acagccgcac ccatcgcggg gcgaatcagc
     7681 aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg
     7741 aacagtacca gcaactgtgg ttcccggctt atggactgct cccccgttgg caccatgccc
     7801 gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct caccctgacc ttttatcagg
     7861 atcacagtga gcatcgggtg attgccggga tcatgcagca gattctggca agtcaccagg
     7921 tcacgctgga aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg
     7981 atatctggct taatagcgcc aactttacgc tgccgctgga tttttcgctg ttcgcgcact
     8041 tgtgcgaggt gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc
     8101 gctggcgcaa tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagtaaag
     8161 caatggtgcc gcttatccac cactggctga tcattcaggg acaacgcagt atgcgcggcc
     8221 tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccaccggatc
     8281 catgattgct ggtagataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg
     8341 caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga
     8401 tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa
     8461 tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt
     8521 tatacctacg attccttcgc cgccgactgg gggcctggtc caaagatcaa aaaagctttt
     8581 gaagccgact gtaactgcga actgaaactg gtggcgctgg aagatggcgt ttcgcttctc
     8641 aaccgtctac ggatggaagg caaaaatagt aaagccgatg tggtgctggg gctggataac
     8701 aatctgttag acgccgccag taaaaccgga ctatttgcca aaagcggtgt ggcagcggat
     8761 gccgttaacg ttcccggcgg ctggaataat gacactttcg tcccgtttga ttacggctat
     8821 ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg
     8881 gttgagagcg atcagaactg gcgggtgatt tatcaggatc cgcgtaccag cacgccgggg
     8941 ctgggtctgc tgttgtggat gcaaaaagtc tatggcgatg acgccccaca agcctggcaa
     9001 aaactggcga agaaaaccgt cacggtcacc aaaggctgga gcgaagccta cggcctgttt
     9061 ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc
     9121 gaagagaaaa aagataacta cgccgccgcg aactttagcg aaggtcacta tctgcaggtg
     9181 gaagtcgccg cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag
     9241 tttatggttt ctccggcttt tcagaatgcg atcccaaccg gtaactggat gtatccggtg
     9301 gcaaatgtca cgctgcctgc cgggtttgaa caattgacca aaccggccac cacgctggag
     9361 ttcacaccag ctgaagtggc ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc
     9421 gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtggtag
     9481 cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg
     9541 cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc
     9601 tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc
     9661 gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc
     9721 tagttgctgt tttcggcatt cttagcgtct atggtcgtca gggctggctg gcatcgctct
     9781 gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc attttgctgg
     9841 cgcacgtatt ttttaatctg ccgatggcga gccgcttatt actccaggca ctggaaaaca
     9901 ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc
     9961 gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg cttatcttta
    10021 tgctctgttt cgccagcttc gccaccgtgc tatcgctggg cggcggtccg caggcaacca
    10081 ctatcgagct ggctatttat caggcgctga gttacgacta cgatcctgcc cgcgcggcga
    10141 tgctggcgct gctccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa
    10201 gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc
    10261 tgcatagccg catttgcgac acggtgttaa tagtgctggc gctgctgctg ttgctgccac
    10321 cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac
    10381 cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggtgtattgt
    10441 gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa
    10501 tgctggcggg tcaggcgctg gagatgagcg gcatgttgat cctcgccatg ccggggattg
    10561 tgctggctac cggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg
    10621 acggcattgt gattttcacc aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg
    10681 aaaacccgat gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg ctgggcattg
    10741 aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg
    10801 cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta
    10861 acgatgattt ccgtaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc
    10921 aggacggcgc ggtcaccgcg ttaattctgc tactactctg ttttctgctg tttaccgtga
    10981 ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct ttatcaccat
    11041 ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcaat cctcgggcca
    11101 agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc
    11161 ggttcgctga ctatcgatgg tgttgatcac accacgacgc cgccgtcacg ccgtccggtg
    11221 tcgatgctgt ttcaggagaa caacctgttc agccatctga cggtcgcgca gaacatcggg
    11281 ctggggctaa atccggggtt gaaactgaac gcggcacagc aggagaaaat gcacgcgatt
    11341 gcccgtcaga tggggattga taatttaatg gcgcggttac cgggcgagct ttccggcggt
    11401 cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat tttattgctc
    11461 gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgctgac gctggtgagc
    11521 acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg
    11581 gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc gcatcgcctg gcagggtaag
    11641 actgaagaat tgttgagcgg taaggcgagt gcttcggcaa tattggggat tacgggttag
    11701 tatgcactgt cagatgcggc gtgaacacct tatccgacct acggacagtg cgttttgtag
    11761 gcctgataag acgcggtcag cgtcgcatga ggcaacccgc acaagacaat cccctaaacc
    11821 ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc
    11881 accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa acggccgcgc
    11941 gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc
    12001 cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc
    12061 tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac
    12121 ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc
    12181 agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga
    12241 atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt
    12301 ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag
    12361 atccagtcgc ccagcagaca accgacaatc cctgccagcc aagcgtgcca gaaacttaac
    12421 tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg tagaatcaaa
    12481 ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg
    12541 gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga
    12601 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
    12661 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
    12721 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
    12781 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
    12841 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
    12901 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
    12961 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtacac gattatccat
    13021 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
    13081 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
    13141 aaacaggtcg ctgaaatacg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc
    13201 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
    13261 ctggtgatac cattcgcgag cctccggatg acaaccgtag tgatgaatct ctcctggcgg
    13321 gaacagcaaa atatcacccg gtcggcagac aaattctcgt ccctgatttt tcaccacccc
    13381 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
    13441 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
    13501 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
    13561 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
    13621 ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
    13681 caggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
    13741 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
    13801 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
    13861 tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg
    13921 tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt
    13981 atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc
    14041 cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg
    14101 tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
    14161 cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg
    14221 cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt
    14281 tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt tattccagcg
    14341 aatggttctg ggcaaaaatc ctgcatgtga ctcgccaaga cagcgccgtg gcgcaatctg
    14401 ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt accacccgcc
    14461 cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg cacgaaagct
    14521 ggggcggctt gccgccagcc agtttctttg atgagctgga cccgatcctc aatcgccatt
    14581 tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc accttatgcc
    14641 cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg
    14701 actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta aaagttatcg
    14761 gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag cgggcagtta
    14821 aaggtatttg cggtcaggtt gatggcagca tggtgcctgg atttatcggt ctggaagcag
    14881 gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc tggccgctgg
    14941 aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc cagaaacaac
    15001 tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg
    15061 tgctcgactg gtttaacggt cgtcgcacgc caaacgctaa ccaacgcctg aaaggggtga
    15121 ttaccgatct taacctcgct accgacactc cgctgctgtt cggcggtttg attgctgcca
    15181 ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc gccgtcaata
    15241 acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg
    15301 acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg
    15361 cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa
    15421 tggccagtgc ggtagagaaa accctgcaac cgcgcagcga acaggcacaa cgctttgaac
    15481 agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt
    15541 ccgccccggc acaggctgcc caggccgttg cgactctata aggacacgat aatggcgatt
    15601 tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg cccggaaacc
    15661 ctgcgtcagg tcacccaaca tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa
    15721 ctgccctaca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat caccgctatt
    15781 tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct gcacaccttc
    15841 tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt gctgcaattc
    15901 cacacccagt tcaacgcggc gctgccgtgg gacagtatcg atatggactt tatgaacctg
    15961 aaccagactg cacatggcgg tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa
    16021 catgccgtcg ttaccggtca ctggcaggat aaacaagccc atgagcgtat cggctcctgg
    16081 atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg atttggcgat
    16141 aacatgcgtg aagtggcggt caccgatggc gataaagttg ccgcacagat caagttcggt
    16201 ttctccgtca atacctgggc ggttggcgat ctggtgcagg tggtgaactc catcagcgac
    16261 ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac gcctgccaca
    16321 caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga gctggggatg
    16381 aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga agatttgcac
    16441 ggtctgaaac agcttccagg tctgcccgta cagcgtctga tgcagcaggg ttacggcttt
    16501 gcgggcgaag gcgactggaa aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc
    16561 ggtctgcagg gcggcacctc ctttatggag gactacacct atcacttcga gaaaggtaat
    16621 gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgccgt agaagagaaa
    16681 ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc ccgactgatc
    16741 ttcaataccc aaaccggtcc agcgattgtc gccagcctga ttgatctcgg cgatcgttac
    16801 cgtctgctgg ttaactgtat cgacacggtg aaaacaccgc actccctgcc gaaactgccg
    16861 gtggcgaatg cactgtggaa agcgcaaccg gatctgccaa ctgcttccga agcgtggatc
    16921 ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc
    16981 cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac ccgcctgcca
    17041 gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc
    17101 acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct acacgctgcg
    17161 attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg
    17221 cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcgaagc gcatccggca
    17281 cgaaggagtc aacatgttaa aagatctcaa acgccaggta ttagaagcca acctggcgct
    17341 gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg
    17401 cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat
    17461 ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga
    17521 cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac
    17581 gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag caaccggcac
    17641 cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga
    17701 aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca
    17761 gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg
    17821 gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat
    17881 ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga
    17941 taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa
    18001 aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc
    18061 agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca
    18121 gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa
    18181 gagtccgtgg cgtttattca cgccgatcag gttccccgcg ctcagcatat tttgcagggt
    18241 gaacaaggct ttcgtctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat
    18301 ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgtct gcgtgaaggt
    18361 ggcgttaccg tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt
    18421 atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt
    18481 ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc
    18541 acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat
    18601 atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc
    18661 agccgcccgc tgctgctgga aaaactcaac gcctggtttg caaaccacga tcccgatgtg
    18721 atcatcggct ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt
    18781 taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc
    18841 tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag
    18901 gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag
    18961 ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc
    19021 cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg
    19081 acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgagc aacggtgaac
    19141 ggcctgccgg tggatcggca cggcggttcg gtggcagcgt ttggtcatct ctattttccg
    19201 cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc
    19261 cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac
    19321 tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtt
    19381 gaaggcatgg cgcaacctga tccagagcac agtaccgaag gttttctcga tgcctggttc
    19441 tcacgggaga aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa
    19501 gccaaacgtc agggtaataa gccgctttcg caggcgctga agatcattat gaatgccttt
    19561 tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcgcctggc atcgtcgatc
    19621 accatgcgtg gtcatcagat catgcggcag accaaagcat tgattgaagc gcagggctac
    19681 gacattatct acggagatac cgactcgact tttgtctggc taaaaggtgc acattcggaa
    19741 gaagaggcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa
    19801 acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac tcatttctgc
    19861 cgttttctta tgccaaccat tcgcggagct gataccggca gtaaaaagcg ctacgccggg
    19921 ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc
    19981 gactggacgc cgctggccca gcagtttcag caggagctgt acctgcgtat cttccgcaac
    20041 gagccatatc aggagtacgt ccgccaaacc atcgacaaac tgatggcggg tgaactggat
    20101 gcgcggctgg tttaccgtaa acgccttcgc cgtccgctga gcgaatatca gcgtaatgtt
    20161 ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa atcaaaagcg cggtcgcccc
    20221 ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg cccggagccg
    20281 ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca gctacaaccc
    20341 gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat gaccgggcaa
    20401 cttgggctat tttgagcaaa aaaaagagtt cgccagatac cattttgatg cgtgacgaat
    20461 gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa caccactacc
    20521 tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc cgaacacata
    20581 tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg ggacttggaa
    20641 ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact ggtgaaaacc
    20701 gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct ggtgatacca
    20761 ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc
    20821 tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg cgtgaagttt
    20881 tccttgatag caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg
    20941 accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc
    21001 gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gatcccgcat cagctcaaca
    21061 tcgctcatga tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt
    21121 tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct ggcgctgctg
    21181 aacgtgtgct gattatcgtc ccggaaacct tacagcatca gtggctggta gaaatgctgc
    21241 gccgtttcaa cctgcgcttt gctctgtttg atgatgagcg ttatgccgaa gctcagcacg
    21301 atgcttacaa cccgtttgac accgaacagc tggtgatttg ctcgctggat tttgcccgtc
    21361 gtagcaaaca gcgcttggaa catctctgtg aagccgaatg ggacctgctg gtggtcgatg
    21421 aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag gccattgaac
    21481 aactggcaga gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa cagctgggga
    21541 tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc
    21601 agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc atgctgctgg
    21661 caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc ggcgagcagg
    21721 atatcgagcc gctgttgcag gcagcaaaca gcgacagcga agatgcccag agcgcccgtc
    21781 aggagctggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca
    21841 cgcgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaag ctgccgctac
    21901 cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt aaaagtgcgg
    21961 aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt gaaggtgata
    22021 acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac ctgaccagcc
    22081 atcgctctca gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg caactggagc
    22141 aggtactgcg cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt atgtcgatta
    22201 tcgaacgtga ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca caggtactgc
    22261 tgtgctcaga aatcggttct gaaggacgta actttcagtt cgccagccac atggtgatgt
    22321 ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg gatcgtatcg
    22381 gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct cagtcggtgc
    22441 tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg accggacgca
    22501 ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg gatcaaaccg
    22561 aaggctttga cgatctgatc aaaagctgcc gcgagcaaca tgaagcgctg aaagcacagc
    22621 tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa aaagcccagg
    22681 aactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc ttcgccatga
    22741 acctgttcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg atcgtgctga
    22801 cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat ggcatcacca
    22861 tcacctttga tcgtgaagtg gcgctggcgc gtgaagatgc acagtttatt acctgggagc
    22921 atccgctgat ccgcaacggt ctggatctga tcctttctgg cgataccggt agcagcacga
    22981 tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa ctgatttatg
    23041 tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca ccgacgccgg
    23101 tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag tttgaaacct
    23161 ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt aacgccgtgc
    23221 agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa tctgcccgtg
    23281 cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc
    23341 tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc gccattgaga
    23401 gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ttggcgtctg gatgccctgc
    23461 gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa actacaatcc
    23521 accgcaggaa ccctggttgg ttatcctgta tcaggatgac catattatgg tggtcaacaa
    23581 gccgagcggt ttgttgtcag tgccgggtcg tctggaagag cacaaagaca gcgtgatgac
    23641 gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata tggctaccag
    23701 cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac gccagttccg
    23761 cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat cccccgcaga
    23821 aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac agaaagtctg
    23881 ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg cggcggataa
    23941 cacggcaaga gtggtgttaa aaccgattac cgggcgttcg catcaattgc gtgtgcatat
    24001 gctggcgctg gggcatccga ttctcggcga tcgtttttat gcatcaccag aagcgagagc
    24061 gatggcaccg cgtttgttgc tacatgcaga gatgttgacg attacccatc cggcgtatgg
    24121 caatagtatg acgtttaaag cgccagcgga tttttaatcg tcacgctggc gcagttcggt
    24181 aggcggataa ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct gatgcgacgc
    24241 gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct gctgctttat
    24301 cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctcagg
    24361 cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct tacggtaggc
    24421 acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac aggcatcttc
    24481 cagcgtcggg ccacgctgcg cttgctgcca gttaccaccg ccagattgct gctgataacc
    24541 gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga
    24601 gatccctaat tcttcagcaa tgacatacag caccgcccgt tcattcgggt gcagtgaacc
    24661 atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc
    24721 aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattat ctgattttcc
    24781 cacccggaac gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat
    24841 caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg
    24901 ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa
    24961 ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac
    25021 tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca
    25081 atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc acaatataaa
    25141 ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata atttgcatta
    25201 tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca
    25261 ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga
    25321 tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga
    25381 ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca cagtgcatgt
    25441 tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga
    25501 ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg
    25561 tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag
    25621 aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat gtccattacg
    25681 acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata
    25741 ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggtcgcggt aaagcggacc
    25801 tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc tttacctcct
    25861 gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag
    25921 aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg atcttttata
    25981 gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg
    26041 ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg aacatcgcgc
    26101 caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac atcatgtggg
    26161 agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc
    26221 cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttat
    26281 tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc gactacacca
    26341 aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg
    26401 gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt
    26461 caaccaagca gttccaggtt ttcagcgaac agaacaccag tagctactcg gcagagccgc
    26521 agttagacgt taattactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc
    26581 aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg
    26641 aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt
    26701 ttctggcaac ccattatcag caaaccaatc ttgactggta taactccaga aacacgacca
    26761 agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc aaaatggtct
    26821 ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc
    26881 agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctgc
    26941 tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg
    27001 cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg
    27061 aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg
    27121 acaacataac atgggagaat gacgacaaaa cgggctcact ggtgtgggca ggcgacacct
    27181 actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg
    27241 ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac
    27301 agctgaatta ccattacgcc agcccggaat atattcaggc tacgctgcct aagtactatt
    27361 ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc agcaggccaa
    27421 ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg aacaagcaag
    27481 ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg
    27541 agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg
    27601 gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc
    27661 tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc
    27721 cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt
    27781 atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc
    27841 gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa
    27901 ctgaacgctg cgcaagcaag gcagcagctt cctgatgacg cgacgctgcg ccaccaaatc
    27961 atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa
    28021 atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg
    28081 ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac ttatcgtaac
    28141 cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc
    28201 accatcctgc cacaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc
    28261 agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacctctgat
    28321 caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg taacggcgct
    28381 gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa cggcggccag
    28441 atggggtggg gccgtattca ggagttgccg gggatcttcg cccaggcatt aagcaccgcg
    28501 aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat tctgaaagtt
    28561 aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt
    28621 ctgctgaaac cgtcgccgat catgactgac gaacaggccc gcgtgaaact ggagcaaatt
    28681 gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctcgcag
    28741 gatccaggtt ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat
    28801 ccagccttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc accggttcac
    28861 tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac
    28921 gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc ggaagaagca
    28981 gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag caactaatgg
    29041 ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag
    29101 ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc
    29161 tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca
    29221 actcccctgc acaaccgcaa actacgggca cattaacgct acttcctgtc gcgctacgtg
    29281 aatctgtcac tgcggggcag ttagcgattg aaaatgggca ttacgtggtg gagacgctgg
    29341 cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc
    29401 ataaaggcgt cattaacgac gcaagcattc cgtttaccgg tcataccgag tttttcgaag
    29461 agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc
    29521 tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcacttttgc
    29581 acgaagtgat tgctattttg catcacgatt tgcgtacaaa atttggtatt gccgaaccgc
    29641 gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag
    29701 agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg aaactcaacg
    29761 ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc
    29821 tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg
    29881 tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg
    29941 aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca
    30001 tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa
    30061 acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat
    30121 taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg cattgaccga
    30181 gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg atctggcggc
    30241 acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc aggatgcgat
    30301 gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa
    30361 cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat
    30421 tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa
    30481 cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccggt
    30541 actggaagtt ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg
    30601 cctggtccct catgcaacga tgcctcaccc ggttaaagat gttcgcgtgt tgagccgcat
    30661 caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt
    30721 tagcgtcgag gtgttaaccg gaatggggat cgacccggcg atgcgagcgg aaaatatctc
    30781 tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag
    30841 ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca
    30901 caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg
    30961 gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt
    31021 gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa
    31081 gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac
    31141 tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc
    31201 gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat
    31261 ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc cgggaaagat
    31321 accctctggc tgacgggcga tctggtcgcg cgcggcccgg gttcgctgga tgttctgcgc
    31381 tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
    31441 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg
    31501 gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagcctct gctgcaaatc
    31561 gacgaagaga aaaagctggt gatggcccac gcagggatca cgccgcagtg ggatctgcag
    31621 accgccaaag agtgcgcgcg cgatgtagaa gcggtgctgt cgagtgactc ctatcccttc
    31681 tttcttgacg ctatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg
    31741 ggaagactgc gttttatcac caacgccttt acccgtatgc gtttttgctt cccgaacggt
    31801 caactggata tgtacagcaa agaatcgccg gaagaagccc ctgccccact gaaaccgtgg
    31861 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg
    31921 ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg ataccggctg ctgctggggt
    31981 gggtcattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg
    32041 cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatacagg aaggccggat
    32101 aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaac ttaccgccgc
    32161 tccagaatct caaagcaata gctgtgagag ttctgcgcat cggcgtcgtg gaattcgctg
    32221 aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct
    32281 gcgtcgatat gcgtcagata cagtttttgc gcttttggca ggaactgctc ataaacgcga
    32341 ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcaatggc ttcatccacc
    32401 gatttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg
    32461 cgtcctggca acggacgacc gattgattcc caagtatggc ggcccataat cacgggttta
    32521 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt
    32581 tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat tgagatttcc
    32641 cgataaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga
    32701 tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctccccc tggactcgcc
    32761 gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttacg tcactcttta
    32821 ttaagatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt taccttcttc
    32881 tgttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct cggtgatgat
    32941 ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg tgtcattctc
    33001 aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat cggcacgttc
    33061 tcgcgcttca tacggcccca gacctaaact ttccagcgcc agacgcccgg ttttcagcgc
    33121 accttcaaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg
    33181 atcgacatcg cgggcgcggg caataatctg caaatgcggg aaatgttctt tcaccatctc
    33241 cgtcagttgc aggttggttt gcggatcgtc aatagcgtta atcagcactt ccgctttcgc
    33301 cgctccggca gattccagta aatccatccg cgtggcatcg ccataaaaca ctttcatacc
    33361 aaatttacgc agcgtttcga tatggtccgg atcgtgatcg agcaccacca ttttcacccc
    33421 gctggagagc agtaaacgtc cggtaatctg tccaaaacga ccgaatccgg cgataatcac
    33481 gcgcggctgt tcttcatcga tctcatcggc ttcacgcgcc tcctccgtag aagattgctc
    33541 aaggcgattg aggatcacca gcagaatcgg cgttgctgcc atcgacagcg ccaccgccag
    33601 ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg caccaaatac
    33661 cacaaaggca aactcactgc cctgccctaa caacaccgca aaccagcgac gctgtttatt
    33721 tggcacctgc aacggtcggg caatcagcca cagcatggcg attttgatga tgaggaaacc
    33781 gagcagcaaa atgacaatgc gcaatgggtt ttcaatcagc gtgccaaagt ctatcgacat
    33841 gccaacaccg atgaaaaaca gccccaacag caaaccttta aacggttcga tatcgctttc
    33901 caacgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg cgcccatcgc
    33961 catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacaagga ataacgccac
    34021 ggcactaaac acttcccgca agccagagcg ggcaacaaaa cgcagcgccg gacgcgtaac
    34081 atagcgcccc agcaatacca ccagtaccag cgcacccgcc acttttaacg ccgagagagc
    34141 aaatgcgccc atcgtcgtcg aggcactgct cgccgccagt agcgggatca tcgccaccag
    34201 cgggattgcc gcgatatcct ggaacagcag cactgcaaag gcactgcggc ccatttgcgt
    34261 caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgttgatg agagcgccag
    34321 cgtcataccg atcaattccg cgacctgcca gcgcagccca agtaacatac agaacagccc
    34381 cagcaggcca ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag
    34441 cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca gcaccacccc
    34501 aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc cccacgggcc
    34561 aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac gtaccgcaat
    34621 gggtacaatc agcgccgccg atccgaggta gatcagcgcc tgaaccagcg tatgactatc
    34681 catgatgggc ctcctgccat tccagcagac gttgcttata gtggcgcgcc tgcccttcga
    34741 gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc
    34801 cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc ggatgcgcac
    34861 caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat
    34921 gcagcgccgt gccgccgtga ccgtaagccc agccgtgcga caaaacttta tcgatccaaa
    34981 gtttaagaag cggaggaatg ctgtaccact gcatcgggtg ctgccagacg atcagatcgg
    35041 cgcgagagag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga tagagttgat
    35101 aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc cgtttattcg
    35161 catgggaatg atgcggatac ggatgcgcat aaatgatgag gatcatagtt attagcccgc
    35221 gtgtttacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa
    35281 taatattgat tcagaaaaga gagaaatctg aacaaataat cccattctct gcccgcggat
    35341 cgtcatattg ctggtctgcg ccagtgacat tcctcgagtc tccggggcaa acgctacgga
    35401 aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac tgataccgta
    35461 attattgata aatatcggca gtgcccaggt cgaaacaatg gtgccaatac gacttaagga
    35521 cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat taggatagag
    35581 ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg ccatcactac
    35641 cagccagatc cccatatccg ggattagccc caaaaccgcc agcgccagcg tcatcatggc
    35701 aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca tcggcggaat
    35761 acagccgagc ataaagaaca ggctaatcac cacattccct agtgccgcgt ttttgccaac
    35821 ccccaatccc aacgaaccaa cgatttgcgg gccaaaggtg taaatggcga acattgggat
    35881 cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa
    35941 cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa
    36001 cagttttatc atcatctctt cgcactcttt tactcgccct ttgcgtaata accagcgggg
    36061 agattctggc agttcgaatc gaccaatcaa aatcaacaaa caagggatcg ccgcgctacc
    36121 cagcatccag cgccaaccac cttccacatc ataaagccag tagccgacca gatcggcgca
    36181 ggtcgcgccg acataccaca tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt
    36241 actggagaac tcggtgatca tcgaagtggc gatgggataa tctgcaccga tgacaatgcc
    36301 gatgagtacc cgcatcacca acagttcgac gggggatgaa acacacatcg tcgccaccga
    36361 tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga ctttatcgga
    36421 gatatagcca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa
    36481 gccaatccag tcagcgtcca gtttcagcgc cggagtcagt tgctccagcg ccacgccaat
    36541 cattaccagt acataaccat ccagaaacgg accaccgctt ccccacagca aaatgcggcg
    36601 gtgaatagag gagaatttga gatcgtcaaa gtttctggac ggttgcatgg gttattcctg
    36661 tttttttgca atgagagtca gtacggcgtt acgtatcaaa ccaacatcag ccgtaacgga
    36721 actccacacc aaaggtgccg cgcgggtatt cccactgttc cagcgccgac cccagcccca
    36781 gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat
    36841 cctgcttctt gtacagacct gcggggcacg ctttcaccag cagctccagc acctgtttat
    36901 cagcatcggc cttcacaaca atgtgcggat gctcttcatc gacattgaat ttattgacgc
    36961 ccagtttgac gtccacattg acgggagaag tcataaaacg gtcactcctt tcatgccatc
    37021 cttgatcaga ttgatgaagc ccactttctt gccgtggcgg aggattttct tgcgcatcag
    37081 ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg
    37141 gttgccgcta aacatgcgtg ggttatcaag gaacgccggt agtttctggt acatgcgcat
    37201 atcgcgcagc gggccactct ccagatgctg acgatattcc gcgagttttt gcttactgaa
    37261 atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca gcttccccgg cggcaatcgc
    37321 cagatccata ccgcgaatgg taaaaccgag gttcatacac attccggcgg catcaccggc
    37381 aatcaatacg ccgtcaccga ccagctccgg cagcatgttg atgcctgctt ccggcactac
    37441 gtgagcggaa tattccacca gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgctg
    37501 tttgaaatct tccagcattt gcggcaccga ttttttcgcg tcatgcagat gatgcagacc
    37561 acaaaccagc cccagcgaca gggtattttc attggtataa aggaagccgc cgcccatcag
    37621 gccatcggtg ggtgatcccg caaacagaca agccgcgccc tgattaccct gcaactgaaa
    37681 acggtcttcg attaccgact tcggtaactc gatcagttcc ttcacgccaa ccgccacatc
    37741 cgtcggttta acgcgttttg ccatccccaa cttttcggca aggatggagt tcaccccatc
    37801 agcaaggagc accgttttcg cttcaatcac atcgccatcg gcttctacac cgacgacttt
    37861 gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta attaactgcg cgcccgcttc
    37921 ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc aaaacggagt aagaacgctg
    37981 ggatggcgag gtttcgtcac cgttgcagta gtccatagtc atcgctgact tttccgtcat
    38041 aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg gcggaatcgg cgaaacctgg
    38101 aataatgtgt tccagactgt gggcatagag acgcccgcca gtaacgttct tggcacctgc
    38161 ggaattgcca cgctcgataa ctaacacttg cgccccttcg cgggcgagca ccagtgcggc
    38221 aaccgaaccg gcaagccctg caccgacgat gatggcgtca aagatatctt cggacataag
    38281 agctccctgt cagcggcaga gccgtaatgc agccctgcca gagtggatca acgcgctaaa
    38341 gctgcggtca gtgccggaag gatcttcacg gcatcgccaa caatgccgta atccgcgtac
    38401 tggaagatcg gcgcattttt atctttattg atggcgaaaa tggtttgcga cgcgttagcg
    38461 ccaaccatgt gctggatcta cccggagatc cccaccgcca ggtacagttc aggtttcagc
    38521 atcaggttgg agataccgac atagcgttcg tgctccatcc atttttcgtt ttctgccacc
    38581 ggacgagaac aggctaactc cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg
    38641 ttctctttgc tgccaatgcc gcgaccgacg ctgaccacca gacgggcttt gtcgagatcg
    38701 acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc
    38761 cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg gctgagccgc atcgaacgtg
    38821 ccgctgctga tggtcagtac cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga
    38881 ccgccgtaaa ccatgtgttt cactgttgct ttaccgtcct gtacgctgac ggtgctggca
    38941 tcgttagaca ccgccgcttt aaggcgataa cccagttttg ccgccagtaa tttgccgcga
    39001 cgggtgtttg gcaacagcac cagaccgtca gcgccgtgct ggcgaatagt gtcagccatg
    39061 acaccggcgt aatcttcgat catccgatcg tccggtttgc cgtttaattt ccagacatga
    39121 ttagcgccga gctggattgc ctgtgcgccg tcggcatcat tgaggacaaa ggcattgatt
    39181 tgattagcta aagcctgcgc accgttcatc agttccggca gacgagaggg ggtatcgctg
    39241 aatacccaga cttgagaaaa cgtgttcata gcatcccctg taattaaatg actttgcgaa
    39301 gattttcagc aaatgcggcg atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac
    39361 gctcgcgctg tttcggcgcg gcaacctgtt gttcagacca ggcgtccacg gcgttaaaac
    39421 caatatccgc cgccgaccat acctggactg gctttttcgc cgcgccgaga atggctttca
    39481 tcgaaggaat ttgtggggag ttgatatcag tggaaacagc aacaaccgca ggcagcggaa
    39541 tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac ggtgagggta tctgccgtca
    39601 gggagataat tttgctgacg ccgttaactg ccggaatatt gaggatttcg cccaccagca
    39661 gaccaacctg ctgggcataa aggtcggaag aaccatcgcc acagaggatc agatcaaagc
    39721 ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg ttgcggcagt gcctgctcga
    39781 actggtcatc aatcaccaca atcagttcat ccgggccgcg cgatagcaca tctttacgcc
    39841 ctttggcgtt ggtcagggct ttaccgccca cacttaaggc tgtcacctgc gcctctgctg
    39901 cctgttgctt tagctggcaa gccgcttcaa tagcgttgag atcgtattgg cttattttgg
    39961 catcggcttt gctgaagtct aatgaaccat cagcattatt gaccgcaata tcctgttcat
    40021 caggcacgca cttatagcaa gtaataatct tcattgcatc tccagaaatc atgaaggaaa
    40081 cgtcgttcat atcggtggtg ttaattacag atatcacgcc ctttaatttg gaaataaaac
    40141 tttaatcacc aatattgaaa atgtcacacg cagataaatc caactttcaa tattgttaag
    40201 ttcctcacca atattgaaaa catggcgtag caaaaagaaa ttttcaatat tgttttatag
    40261 atcaccaata ttgaaagtca gatgctattt ttttacccac aaaagaatat aacaaactga
    40321 ttaataacca aaattaacga aaaacgccct ctgttcactc gaaacaaaaa tgtgacacca
    40381 atcacagaat acagcttatt gaatacccat tatgagttag ccattaacgc gtccacgagg
    40441 ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc aaaataaata
    40501 ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc
    40561 gaaggttttc tttccgccat taataatcgt cggcatactt tgttggctta cagtcagaga
    40621 tctggatgcg gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca atgtatgggg
    40681 atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc tggtgtttgg
    40741 cccgtatgcc aaaaagcgtt taggtaacga accgccagaa tttagcaccg ccagttggat
    40801 ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat cgattgagat
    40861 ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag gagcgaaaga
    40921 gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca cttacagctt
    40981 cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga ttcgccccag
    41041 ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg gtaccatcgt
    41101 cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg gccttgccac
    41161 gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc tgcaactgga
    41221 cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt gcggtctgca
    41281 aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga tgctgggttg
    41341 ggtgttcatc gtcagcggtg ccagcttcat catgaactac ttcaccgatt cggtggggat
    41401 gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta aaggcggctt
    41461 cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttacgcca tccagatgag
    41521 tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct tcggcatggt
    41581 gatggggctg acagcatcca cctggatcct gtggaccgtg ctcggtagta acactctgct
    41641 gttgatggat aaaaacatca tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg
    41701 cgccatcatc gaaacctggg ccgctctgcc actcagcacc gccaccatgt ggggcttctt
    41761 catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata ccctggcgat
    41821 gtctacttgc cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc gtatcggttg
    41881 gtcaattctg gttggcatta tcggtattgt tctgctggcg ctcggcggcc tgaaaccgat
    41941 tcaaaccgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta tggtgacgct
    42001 ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca aaatatcaag
    42061 aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg
    42121 cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc gtgacagcgt
    42181 ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc tgctgatccc
    42241 tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt ggatggagct
    42301 gggacgtctg ggggcaccaa cctatgtact gtaccagctg ccgggcgggt tcaatacctt
    42361 cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg gcaccggtaa
    42421 gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa
    42481 aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt gttttattac
    42541 cagtagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt ctccggacaa
    42601 acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact
    42661 tgagaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg atgtggaact
    42721 ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca aagaagagtt
    42781 cgaccatgaa cgtttcctgg tagccctgac caactacggt acggcgatgt gcgcctttga
    42841 agatgcggcg cgctacgcca accagcgcgt gcagtttggc gaggctattg gtcgtttcca
    42901 gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga aaaacatgct
    42961 gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg cagcgatgtg
    43021 caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc aggtgctggg
    43081 cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga
    43141 ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg ctgggtcgtg cggtgctgaa
    43201 gcaataccgc taagtccgtg cgctgcctga tgcgacgctg acgcgtctta tcaggcctac
    43261 gggagcacga atgtaagccg gataaggcgt ttacgccgca tccggcagtc atgcgctaca
    43321 taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg accgctggcc
    43381 ggattgcgcg ttgtcttctc cggtatcgaa atcgccggac cgtttgccgg acaaatgttc
    43441 gcagaatggg gcgcggaagt tatttggatc gagaacgtcg cctgggccga caccattcgc
    43501 gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc gttaaatatt
    43561 ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga tatcttcatc
    43621 gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt actgtggcag
    43681 cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg caccgaggag
    43741 tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta cctgattcag
    43801 aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta cttttctggc
    43861 ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac cggtaaaggc
    43921 gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta cttcatgatg
    43981 gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga tccctactac
    44041 gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact ggtgggcatt
    44101 acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcgg tacgccggaa
    44161 atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc actggtagaa
    44221 gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga acgctttgcc
    44281 gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag caatccacag
    44341 tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg
    44401 ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg aatgccctca
    44461 catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa cgacattcag
    44521 gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt cagtcggctt
    44581 gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg caatggatat
    44641 cattggcgga caacatctac gtcaaatatg ggacgatctg gcggacgttt acggtcataa
    44701 aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt atcttgagtt
    44761 aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc gcaaaggcaa
    44821 caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt tcgggctggc
    44881 aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg aaagcgagtg
    44941 gatcttgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaatttt atcctatgta
    45001 tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga cagatgtggc
    45061 acttcccgct gatgatggag tgagttcatt tactcaactg aaaaatcaac aacctgccac
    45121 cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct tcacctccgg
    45181 caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct tcgctggata
    45241 ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacatgacgg taatgcctgc
    45301 gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg gggccacctt
    45361 tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggcgcaga agtaccgcgc
    45421 caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc cgccttcagc
    45481 gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt cggagcagga
    45541 aaaagacgca ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg ggatgacgga
    45601 aaccattgtg ggcattatcg gcgatcgccc tagcgataaa cgacgctggc cgtcgattgg
    45661 tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc cgctcccggc
    45721 aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct tcaaagagta
    45781 ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc tacataccgg
    45841 cgataccgga taccgcgacg aagaaggctt tttttatttc gtcgatcgcc gctgcaatat
    45901 gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca tcgccaccca
    45961 tccgaaaatt caggacatcg tggttgtggg tattaaagat tcgattcgcg atgaagccat
    46021 caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat tttttcgctt
    46081 ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca gaaaagatct
    46141 gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga aacgtcaggg
    46201 aactaccctg ccagctaata atcacgccct aaaacaatat gcattttttg cagggatgtt
    46261 gtcgtcccta aaaaagcaaa aatggagaaa aggaatgagt gaatcattac atctgacccg
    46321 caatggatca attctggaaa ttacccttga tcgcccaaaa gcgaatgcta ttgatgcaaa
    46381 aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc aattacgtgt
    46441 cgccattatt accggtgccg gagagaaatt cttttccgcg ggctgggatt taaaagcggc
    46501 agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggttttgcgg gattaaccga
    46561 aattttcaat ctcgacaaac cagttatcgc agctgtgaac ggctacgcct ttggcggcgg
    46621 tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca gcttcgccct
    46681 gccggaagcc aaactgggca tcgttcctga cagcggcggt gtgctgcgtc tgccgaagat
    46741 cctgccgcct accatcgtca atgaaatggt gatgaccggc agacgaatgg gcgcagaaga
    46801 ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga tggataacgc
    46861 ccgcgaactg gctcagcagc tggttaacag cgccccgctg gcgattgcgg cgctgaaaga
    46921 gatctaccgc accaccagcg aaatgccggt agaagaagcg tatcgctata ttcgcagcgg
    46981 cgtgttgaaa cactatccat cggttctgca ttcggaagat gccattgaag ggccgctggc
    47041 gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag ctattacgcc
    47101 tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag tgccgtcttg
    47161 attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc actgcgtggt
    47221 gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg cattatgcat
    47281 ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca tggagcgatc
    47341 ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt aattatggat
    47401 ggcgcggtca ttggcgaaga gagcattgtt gccgcaatga gctttgtcaa agcaggcttt
    47461 agtggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg cagtgttagt
    47521 gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct tgtcgggcgc
    47581 tgtcatgcat cgttacatga aacgcagcca ctgaggcaaa tggaggaaaa tcgcccccgt
    47641 ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg acctggttgc
    47701 tggataagat gctacaacat cgtatctggc aagcaggacg cccggtatca acgagataaa
    47761 attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg ttaagttccg
    47821 tgagcggaac ggcaggaata cgcggtgtct ttcttacgcc gactaatttt tcccgactgt
    47881 tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc agtcgctgac
    47941 actggcatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag cttatttctg
    48001 tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca atatcgaaat
    48061 gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat tcggcgatta
    48121 acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt tcacaaatta
    48181 aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa attgtggata
    48241 tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct tatgtcatca
    48301 aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaaacggtc ctcatcagag
    48361 aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccacg acacgctatt
    48421 atttgatctg tgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca tcggcattca
    48481 gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact ttatattgca
    48541 gcgcactgcg acggatcacg cgggagtctt caatcgcacg acggcctgag gtggtgttga
    48601 tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacgg ccttcatgca
    48661 ccttgtttac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc gtgccgtggg
    48721 tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc acgcgttctt
    48781 tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg gagttgctgc
    48841 ccagctgcgc tttggcgaac gcttcagcga aggtgcggcc cacgcccatg acttccccgg
    48901 tagagcgcat ttctggccct aacagcgggt caacgcctgg gaatttattg aacggcagca
    48961 ccacttcttt caccgagtag tacggcggaa taacttcttt ggttacgccc tgctcagcca
    49021 gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg gtggctttgg
    49081 agacgaacgg aacagtacgc gccgcacgcg ggtttacttc aatcaggtaa acttcgttgt
    49141 ttttcaccgc aaactgcacg ttcatcagac cgcgcacctg caattcgaag gccagtttct
    49201 gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtacgct ggcagcgaac
    49261 atgcggagtc accggagtgc acacccgcct gctcgatgtg ctccatgatg ccgccaatca
    49321 gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca tcaaggaaat
    49381 gatccagcaa cactggcgca tcgttagaca cgctaaccgc cgtctggaag tagcgacgca
    49441 ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa gacggacgca
    49501 ccaccagcgg gtagccaatc tcttttgcct tctcaactgc catttcaata gcagtaacgg
    49561 tggcgttcgc cggttgtttc agtttcagac ggtcaaccgc atgctggaag cgttcacggt
    49621 cttccgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca gcggcttcca
    49681 gtgcacgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg cctttcggct
    49741 tctcaatacg cacgatttcc agcacatctt ccagagttac cggctcgaag tagaggcgat
    49801 cggaagtgtc gtagtcggta gagacggttt ccgggttaca gttaaccata atggtttcgt
    49861 aaccgtcttc acgcagcgcc agcgaggcgt gtacgcagca gtagtcgaat tcgataccct
    49921 gaccgatacg gtttggaccg ccgccaagca ccatgatttt ttcacggtcg gtagacggat
    49981 tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg gcaaactctg
    50041 ccgcacaggt atccacgcgc ttgtagaccg ggtgcaggtc atattggtca cgcagcttac
    50101 ggatttccgc ttcgcgtacg cccgccagtt ttgccaagcg cgcatcggca aagcctttgc
    50161 gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc gccactttct
    50221 cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaatg ttagtcaggt
    50281 tgaagacgcc gtccacagac aggcccgcgc ggaaagcatc ggcgatgtac cagatacgct
    50341 ctgcgcctgc gtctttcagt tcgcgacgga ttttggttaa cgcttccggg tcatccaggc
    50401 tcactttcgg gtcgaagcca gttgcgccca cttccaggcc gcgcagcgct ttttgcaggg
    50461 attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc tgagtggtca
    50521 gacggtcgtt agcaccggcg aatttttcga agttgaagcg aggaatttta gtaaccacat
    50581 agtcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg ttcatcagtt
    50641 cgtctagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg aaaccggtcg
    50701 ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata acaatcaggc
    50761 gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca acgccgattt
    50821 cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg gtcagcgttt
    50881 gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca aagttttcga
    50941 tagagcagac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc tcgtactctt
    51001 tccagccgat cagcgactca tcaatcagca actctttggt cggagagaga gccagaccgc
    51061 gggcgcaaat ttcttcaaac tcttcacggt tataagcgat accgccgccg ctaccgccca
    51121 tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaaca gccagcgctt
    51181 cttccatcgt gtgtgcgata ccggaacgcg cggtttccag accaattttc ttcatcgcta
    51241 cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca ccaatcatgg
    51301 tgacaccgaa ctcttccaac acgccctgac gttccagctc cagcgcgcag ttcagcgccg
    51361 tctgaccgcc catggttggc agcaccgcgt ccgggcgctc tttttcaata atcttgcgta
    51421 ccacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg tcggtcatga
    51481 tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc agggctttac
    51541 atgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc gggcccgcac
    51601 ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta ctcctgatta
    51661 cttagcggtt ttacggtact gctcaattaa cttgataaag tggtcgaaca acggcgcggc
    51721 gtcgtgtgga ccagggctgg cttcagggtg accctggaag ctgaacgccg gcttatcgat
    51781 gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac gcaggtttgc
    51841 aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt
    51901 tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca ttttgacagt
    51961 cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga ataccggaat
    52021 atcggtttcg aggaacttct ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc
    52081 aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt ccgcagaagt
    52141 ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca ggatgttgcg
    52201 cttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt ttttcgcttc
    52261 tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt ctgcggtggt
    52321 cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt tttctaacgc
    52381 cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg
    52441 cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt tatggcgctt
    52501 caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg
    52561 aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat tagtgccgac
    52621 attgccaata tggggataag taagagtaac gatttgacga gaataggaag gatcagtgag
    52681 gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt
    52741 tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata gcgctgactt
    52801 aatcaaaaca ccctccagag aatactcact cactttattt gcatattaat tcacagtgat
    52861 gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg gcattctgga
    52921 gatatgggcg ctaaaagtca acttaatggc aggatttttt atattatttt gtggctcatg
    52981 catgttttac aggcattaag cgacaaaaac atcatctaac ctgttctgga aaacgcttgc
    53041 gcagcaagaa gattgcatga aataaaaacg agcaggaaaa agtggaccaa atggtcaaaa
    53101 tttacattaa aacaacaaaa atagaagccg aaacatgtaa tttttggcag ttatagaaaa
    53161 tttaaaagca taacaccaaa aataaaaggg ctaaaaatta aagcccttta taattaataa
    53221 attaaatgtg ttacttttgc atcataataa acattttgtg gttacaaatt attgagatca
    53281 agcacatctc gcatatcaaa aagaccactt tccttaccac tcaaccacaa agccgatctt
    53341 accgcaccgt tagcaaatgt catgcggctg gacgccttat gggtgatctc cagacgctcg
    53401 ccaatatcgg caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg cacggtagca
    53461 aaaccaatgg tacctggcac acgttcaccg gtatggcctt cacgactgta gaccgcgcaa
    53521 tctttcagat ctttatcaag ggcgtgggcg atcgcctctc ccattgccag tgcggtgcct
    53581 gacggcgcat caactttatg tctatgatgt gcttcaataa tttcgatatc ggtgtagtca
    53641 cccatcactt tggctgcttt ctccagcagc ttaagcatga cgttaacgcc aacgctaaaa
    53701 ttggcggcaa agacaatcgc aatatcggcg gcggcgtcac gaattgcctg tttaccggct
    53761 tcgtcaaacc ccgtagtgcc gatcaccatc cctttgccat gctgccgaca aaaagcgaga
    53821 tggttcagcg taccttccgg acgggtaaaa tcgataaaca catcaaaatc atcttttatc
    53881 gcatcaagac tgctttgcac ggtaacgcct gttttccctg caccagccag ctcaccggcg
    53941 tcgctgccca gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg cacgccttct
    54001 aatgccagtg ccgcctgaat caactggcgg cccatacgcc ccccggctcc cgcgatggca
    54061 acgcggatgt ttgcatcatg catagctatt ctcttttgtt aatttgcata gaccgttttc
    54121 agagtaacca gcgcatcgct acgccgccag ccaaaaacgc tgataattag aaattaatga
    54181 taaacaggga gggatatcag tgaaaggcat gagtcgatga atgactgcat gccgataaca
    54241 tgtgacaggt tacggcacca gagccagcac ctcagccacc cactgttgga agccttttac
    54301 attcagatcc aatgccacct gtacattggc tggtttgccc aggcaaccgt cgatatcaac
    54361 caccgtcgtg cctgaggtaa attcgccttg agtttctacc gcgacaaaac acggcttgag
    54421 ggtgaataag tccgggcgca ccagccaggc gatggcgcag agatcgtgca ttcgcagacc
    54481 gctttgcata ctgccgctac ggtagtggct aaacagggcg tggagcattt ttccggtgcg
    54541 attcaactcc ggtaaagtag cgagatagtc aggggttaat attgcctgat tggtgacatc
    54601 cagaccgcac atgacaattt cgatgccgct acggaagaca caggcagccg cttctggatc
    54661 ggcagcgata ttaaactcgg catttggtgt acagttgccg cgaccagcag aaccgcccat
    54721 gatcaccaga cgacgaatat acggtttaca ttccgggcat tgtaaaagta acagcgcaat
    54781 attggttaac gggccgatgg ccaccagggt aacgggctct ggtgcacgca tcagggcatc
    54841 ccgaatcgcc agaaacgccg gtatcccgag tggctttcgg ttgtgctcaa caaagtcgta
    54901 gccagccatt cccgattcgc cgtgcacaga tgctgcatca cgcggtgcgc gtaccagcgg
    54961 cacagcggct ccctgggcga gcggaatctc cgcattccag aaatgcagca gttgcagggc
    55021 attgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca gttgcaggtc
    55081 gagttcgggg gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc cggggtcagt
    55141 atcgaggaag ataggtaaac gcatgttttc ttcataaaaa atgccggtaa caagaccggc
    55201 attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc gacacgcagc
    55261 tctttcggca cttcgaaaac aatattttct tcacggcctt ccagcggaat ggcttcacca
    55321 ccacccaact gctgcaaacg tgccaccaca ttctgcacca gaatatccgg agccgatgcg
    55381 cccgcagtca cgccgacgca tttaacctct ttcacccact cttcctggat atctttcgca
    55441 tcgtcaatca aaaacgcgtg tttgcccata cgctgggcca gctccgccag acggttggag
    55501 ttggaggagt ttttcgaacc gaccaccaac acaacttccg cctgttctgc cagggcgcgt
    55561 accgcttcct gacggttagt cgtggcgtag cagatgtcat ctttgcgcgg accgacaatt
    55621 ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tgtcatccac cgacagcgtg
    55681 gtttgggtca taaaggagag cttctcttcg tttttgaccg tcagtttcca cacatcgtct
    55741 ggcgattcga ccagatacat tcccccttcg gggttgctgt actgccccat cgtcccttcc
    55801 acttccgggt gcccggcgtg gccgatgaga atagattctt cgccacggcg actggcgcgg
    55861 gcaacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac cgtcaaatcg
    55921 cggcttttcg cttcgttacg aaccgcctga gaaacaccgt gtgcggagaa aatcaggatc
    55981 gcgccgtccg gcacttcgct aatctgctca ataaagatag ccccacgctc gcgcaagcta
    56041 tcgaccacgt agcggttatg caccacttcg tgacggacat atatcggtgc gccgtaaatg
    56101 gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa accacgcggg
    56161 ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca gcacttcaat
    56221 atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa tggagtcgcc
    56281 gttaatttcg cggatcacac caggcatctc actgccatcc attgcggtaa aaagcatgat
    56341 tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt actgaatcag
    56401 gtccggtgac ggcacgccaa acgccgcatc tggctccagc gagaaggtgg ttttgtcgcc
    56461 cactttcagc cccagcaggt gttgctccag cccttcagaa agagaagcat cgcccaggcg
    56521 gaacagcgcc ggtttaccgt tgttgcgagt agactcggcg gtggtgccat cgtcgagttt
    56581 tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca tgcaggtttg
    56641 ctcttatttt tgaggatttt tgccggatgc ggcctacgat ttcgtagcaa tctgtaggcc
    56701 gcatccggca gggtttatta ttgttttttc gctttggaag gtaaaaaccc ttccagcacg
    56761 atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc gaagtgccag
    56821 tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg gtcgaacagg
    56881 ttgcccagcg cgccgccaat aatcagcgcg taagcgatat tgtttagctt ctgcgtggcc
    56941 ttcgagcgat acatcatcac tgccaggagc acgctaatac caatcgcaat accggcaaag
    57001 aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc atagttacgc
    57061 gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc aaagttctgg
    57121 aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag ccacagccag
    57181 cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt tcaccgtcac
    57241 cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc ttgccgacat
    57301 cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc aacgcgactt
    57361 tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta tagtctgcaa
    57421 cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg gtcagtttcg
    57481 ccgccagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca cccactttct
    57541 tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc aacagctcgt
    57601 cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacaggcct tcgtaccact
    57661 caccggtgaa gacgtatttt tcacgttcgc ccggcagata gccccacact tcatcagcgg
    57721 tgaaggagag gattggtgcc atccagcgca ccagcgcttc agcgatgtga tacagcgcag
    57781 tctggcagct acgacgcgcc acgctatccg ctttggcggt gtactgacgg tctttgatga
    57841 tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc tgtaccactt
    57901 cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct ttcgcacaac
    57961 ctacggccca gcgatccagt accaccatct cttccggttt caccatatct tttgctggat
    58021 caaaaccatt caggtttgcc agcaggaagc gcgcggtgtt acggatacga cgatagctat
    58081 cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag tcggttgatg
    58141 ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc ggcgaaacgg
    58201 tgttgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag ccgtgggtca
    58261 gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg gaagacatga
    58321 accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga ccggcaaatt
    58381 ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat acgtcgagag
    58441 tatccggcac tttcacatac tggtcagctt cgtcgccgag gatctctttc gcatcgagat
    58501 cccaccacgc ctggatgccg tcaacttcaa cgcgtttagc cacttcttcc atcagttcga
    58561 gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc ggtacgcccc
    58621 aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac tcgatacgcg
    58681 cctggcccca gtccgggagc cactgcacgc ctttgatctc tttcagtgac tgcgcacgca
    58741 gacctttctg atccatgctg acgaaccact gcggtgtcgc gcggaagatg atcggcgttt
    58801 tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc agcgcgcctt
    58861 tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg ccatccagcg
    58921 tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg gtttccaggc
    58981 cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg tgaacggcac
    59041 cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg aagcccataa
    59101 acggatgggt aaagcgcagc agctcaagct ctgcaccttt taccgtgccg agaatggtgt
    59161 aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc agaatcacgg
    59221 cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag attgcgcggt
    59281 tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg ccgttaacgt
    59341 tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa gcgacgtcaa
    59401 tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca gaacggcagt
    59461 ctacgcacca gtgcaccggc ttcgcgcctt tgtgcaggtg accattgccg atgattttgc
    59521 ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat agtcagatac gggtgcgacc
    59581 agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc tgggtcgccg
    59641 cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc ggcttaccgt
    59701 attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca ggcacatacg
    59761 gcgagtcata accggaaagc cctttggact tcacaataat gtctttcaga atcttgttaa
    59821 ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc agaatgaagg
    59881 tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca gtccaacgcg
    59941 ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct gtttccggca
    60001 aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc ggtttgatta
    60061 cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt aattcatcac
    60121 gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg cgtatttttt
    60181 tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct aacaaatgca
    60241 cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt gccacgccgg
    60301 gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc accggggaaa
    60361 cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct aattcatcac
    60421 cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct gccagagcca
    60481 gattgtcatc agccagcgcc tgacgcacgg cggtgctgct gatacgcaca ccaccttcgc
    60541 aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc tgtaataaca
    60601 agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc acctacggca agaaatttta
    60661 cgcgcaaatg cttcaccaaa agatcgctga tgaaattttg cgcggttaac gccgcaaaac
    60721 gcctgtcgaa acgcacgcac agcacgtaat caacgccaca ctctgcaagg taacgcagtt
    60781 tttcccgcag ccgggtcagt cttgccgggg ctttatcggt agcaaacagt tccagtggtt
    60841 gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct tcctgcaagc
    60901 cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata gtcagcacac
    60961 acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc atgtctggct
    61021 caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa ccggcgattg
    61081 agtaccgaaa tcagaaagaa gtggcttttc attgccatgg agcaaatcac gggaagaaac
    61141 tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt cacaccccgc aagctggtag
    61201 aatcctgcgc catcactacg taacgagtgc cggcacatta acggcgctta tttgcacaaa
    61261 tccattgaca aaagaaggct aaaagggcat attcctcggc ctttgaattg tccatataga
    61321 acacatttgg gagttggacc ttggctaata tcaaatcagc taagaagcgc gccattcagt
    61381 ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat gatgcgtact ttcatcaaga
    61441 aagtatacgc agctatcgaa gctggcgaca aagctgctgc acagaaagca tttaacgaaa
    61501 tgcaaccgat cgtggaccgt caggctgcta aaggtctgat ccacaaaaac aaagctgcac
    61561 gtcataaggc taacctgact gcacagatca acaaactggc ttaatcgcca atttgctgaa
    61621 gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc tgctgctttt gtgatgcgtg
    61681 agagaacaca ctgccggttg ccagatagtc aaaagagaca tgccgttgta ctatctgaac
    61741 tcttcgtggc ttaagaatcg tagaaccatc ggctgataca ttctgaacaa gaatatctac
    61801 tcaatctcca gactccccca caaagaatat ggatattgtg atacacattg aggtattaaa
    61861 cttaagacca ctctattttt gaattaaatt cctatatact ttaaccattc ataaatatct
    61921 cctcattaga cct
//
LOCUS       DAECOD010000010        60419 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.010, whole genome shotgun sequence.
ACCESSION   DAECOD010000010 DAECOD010000000
VERSION     DAECOD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.010"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..322
                     /locus_tag="KKO15_000620"
     CDS             <1..322
                     /locus_tag="KKO15_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6359537.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="KKO15_000621"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="KKO15_000621"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBD6359538.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="KKO15_000622"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="KKO15_000622"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBD6359539.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KKO15_000623"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KKO15_000623"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBD6359540.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="KKO15_000624"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="KKO15_000624"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBD6359541.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="KKO15_000625"
     CDS             complement(3726..4955)
                     /locus_tag="KKO15_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD6359542.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="KKO15_000626"
     CDS             complement(5019..5459)
                     /locus_tag="KKO15_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD6359543.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="KKO15_000627"
     CDS             complement(5564..6334)
                     /locus_tag="KKO15_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6359544.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="KKO15_000628"
     CDS             complement(6331..7257)
                     /locus_tag="KKO15_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6359545.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="KKO15_000629"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="KKO15_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBD6359546.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KKO15_000630"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KKO15_000630"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBD6359547.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="KKO15_000631"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="KKO15_000631"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBD6359548.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KKO15_000632"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KKO15_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBD6359549.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKRPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="KKO15_000633"
     CDS             12964..13311
                     /locus_tag="KKO15_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBD6359550.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRHALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="KKO15_000634"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="KKO15_000634"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBD6359551.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="KKO15_000635"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="KKO15_000635"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBD6359552.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="KKO15_000636"
     CDS             complement(15131..15493)
                     /locus_tag="KKO15_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBD6359553.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KKO15_000637"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KKO15_000637"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBD6359554.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="KKO15_000638"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="KKO15_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KKO15_000639"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KKO15_000639"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBD6359555.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KKO15_000640"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KKO15_000640"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBD6359556.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KKO15_000641"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KKO15_000641"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBD6359557.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KKO15_000642"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KKO15_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBD6359558.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="KKO15_000643"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="KKO15_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBD6359559.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KKO15_000644"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KKO15_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBD6359560.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KKO15_000645"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KKO15_000645"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBD6359561.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="KKO15_000646"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="KKO15_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBD6359562.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="KKO15_000647"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="KKO15_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBD6359563.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="KKO15_000648"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="KKO15_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="KKO15_000649"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="KKO15_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBD6359564.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KKO15_000650"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KKO15_000650"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBD6359565.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="KKO15_000651"
     CDS             complement(36348..36386)
                     /locus_tag="KKO15_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359566.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="KKO15_000652"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="KKO15_000652"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBD6359567.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="KKO15_000653"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="KKO15_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBD6359568.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="KKO15_000654"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="KKO15_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBD6359569.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KKO15_000655"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KKO15_000655"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBD6359570.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRNITLWFWLVESWEGVPWGKEGQPG
                     EWMSLIGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KKO15_000656"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KKO15_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBD6359571.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KKO15_000657"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KKO15_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBD6359572.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KKO15_000658"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KKO15_000658"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBD6359573.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KKO15_000659"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KKO15_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBD6359574.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KKO15_000660"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KKO15_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBD6359575.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KKO15_000661"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KKO15_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBD6359576.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KKO15_000662"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KKO15_000662"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD6359577.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRSEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KKO15_000663"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KKO15_000663"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBD6359578.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KKO15_000664"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KKO15_000664"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBD6359579.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KKO15_000665"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KKO15_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBD6359580.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KKO15_000666"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KKO15_000666"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBD6359581.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KKO15_000667"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KKO15_000667"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBD6359582.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KKO15_000668"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KKO15_000668"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBD6359583.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KKO15_000669"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KKO15_000669"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBD6359584.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KKO15_000670"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KKO15_000670"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBD6359585.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KKO15_000671"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KKO15_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBD6359586.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KKO15_000672"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KKO15_000672"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBD6359587.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KKO15_000673"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KKO15_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBD6359588.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14477 a        16731 c        14844 g        14367 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagcag gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacgc atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggccgtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcacgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc acataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggtggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgatc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatattcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcgactcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgagcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaaa ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgaattttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAECOD010000011        59009 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.011, whole genome shotgun sequence.
ACCESSION   DAECOD010000011 DAECOD010000000
VERSION     DAECOD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.011"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            534..1823
                     /gene="sdaC"
                     /locus_tag="KKO15_000674"
     CDS             534..1823
                     /gene="sdaC"
                     /locus_tag="KKO15_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBD6359589.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPV
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1881..3248
                     /gene="sdaB"
                     /locus_tag="KKO15_000675"
     CDS             1881..3248
                     /gene="sdaB"
                     /locus_tag="KKO15_000675"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBD6359590.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3359..4114
                     /gene="xni"
                     /locus_tag="KKO15_000676"
     CDS             3359..4114
                     /gene="xni"
                     /locus_tag="KKO15_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBD6359591.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4167..5315)
                     /gene="fucO"
                     /locus_tag="KKO15_000677"
     CDS             complement(4167..5315)
                     /gene="fucO"
                     /locus_tag="KKO15_000677"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBD6359592.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5343..5990)
                     /gene="fucA"
                     /locus_tag="KKO15_000678"
     CDS             complement(5343..5990)
                     /gene="fucA"
                     /locus_tag="KKO15_000678"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBD6359593.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6537..7853
                     /gene="fucP"
                     /locus_tag="KKO15_000679"
     CDS             6537..7853
                     /gene="fucP"
                     /locus_tag="KKO15_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBD6359594.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7886..9661
                     /gene="fucI"
                     /locus_tag="KKO15_000680"
     CDS             7886..9661
                     /gene="fucI"
                     /locus_tag="KKO15_000680"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBD6359595.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9770..10586
                     /gene="fucK"
                     /locus_tag="KKO15_000681"
                     /pseudo
     CDS             9770..10586
                     /gene="fucK"
                     /locus_tag="KKO15_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10616..10780
                     /gene="fucU"
                     /locus_tag="KKO15_000682"
                     /pseudo
     CDS             10616..10780
                     /gene="fucU"
                     /locus_tag="KKO15_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="catalyzes the interconversion of alpha-L-fucose to
                     beta-L-fucose; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            10838..11569
                     /gene="fucR"
                     /locus_tag="KKO15_000683"
     CDS             10838..11569
                     /gene="fucR"
                     /locus_tag="KKO15_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBD6359596.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(11613..12713)
                     /gene="rlmM"
                     /locus_tag="KKO15_000684"
     CDS             complement(11613..12713)
                     /gene="rlmM"
                     /locus_tag="KKO15_000684"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBD6359597.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(12706..13101)
                     /locus_tag="KKO15_000685"
     CDS             complement(12706..13101)
                     /locus_tag="KKO15_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBD6359598.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13120..14037)
                     /gene="gcvA"
                     /locus_tag="KKO15_000686"
     CDS             complement(13120..14037)
                     /gene="gcvA"
                     /locus_tag="KKO15_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBD6359599.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14388..14615)
                     /locus_tag="KKO15_000687"
     CDS             complement(14388..14615)
                     /locus_tag="KKO15_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBD6359600.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            14807..16012
                     /gene="csdA"
                     /locus_tag="KKO15_000688"
     CDS             14807..16012
                     /gene="csdA"
                     /locus_tag="KKO15_000688"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBD6359601.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16012..16455
                     /gene="csdE"
                     /locus_tag="KKO15_000689"
     CDS             16012..16455
                     /gene="csdE"
                     /locus_tag="KKO15_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBD6359602.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(16506..17312)
                     /gene="tcdA"
                     /locus_tag="KKO15_000690"
     CDS             complement(16506..17312)
                     /gene="tcdA"
                     /locus_tag="KKO15_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBD6359603.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(17551..18648)
                     /gene="mltA"
                     /locus_tag="KKO15_000691"
     CDS             complement(17551..18648)
                     /gene="mltA"
                     /locus_tag="KKO15_000691"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBD6359604.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            18857..18933
                     /locus_tag="KKO15_000692"
     tRNA            18857..18933
                     /locus_tag="KKO15_000692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18891..18893,aa:Met,seq:cat)
     gene            18967..19043
                     /locus_tag="KKO15_000693"
     tRNA            18967..19043
                     /locus_tag="KKO15_000693"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19001..19003,aa:Met,seq:cat)
     gene            complement(19117..20370)
                     /gene="amiC"
                     /locus_tag="KKO15_000694"
     CDS             complement(19117..20370)
                     /gene="amiC"
                     /locus_tag="KKO15_000694"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBD6359605.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            20602..21933
                     /gene="argA"
                     /locus_tag="KKO15_000695"
     CDS             20602..21933
                     /gene="argA"
                     /locus_tag="KKO15_000695"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBD6359606.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(21995..23821)
                     /gene="recD"
                     /locus_tag="KKO15_000696"
     CDS             complement(21995..23821)
                     /gene="recD"
                     /locus_tag="KKO15_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBD6359607.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(23821..27363)
                     /gene="recB"
                     /locus_tag="KKO15_000697"
     CDS             complement(23821..27363)
                     /gene="recB"
                     /locus_tag="KKO15_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBD6359608.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDTVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27356..30244)
                     /gene="ptrA"
                     /locus_tag="KKO15_000698"
     CDS             complement(27356..30244)
                     /gene="ptrA"
                     /locus_tag="KKO15_000698"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBD6359609.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30420..33788)
                     /gene="recC"
                     /locus_tag="KKO15_000699"
     CDS             complement(30420..33788)
                     /gene="recC"
                     /locus_tag="KKO15_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBD6359610.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(33801..34133)
                     /locus_tag="KKO15_000700"
     CDS             complement(33801..34133)
                     /locus_tag="KKO15_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD6359611.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34118..34525)
                     /locus_tag="KKO15_000701"
     CDS             complement(34118..34525)
                     /locus_tag="KKO15_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBD6359612.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(34522..35085)
                     /locus_tag="KKO15_000702"
     CDS             complement(34522..35085)
                     /locus_tag="KKO15_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD6359613.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNR"
     gene            complement(35076..35546)
                     /locus_tag="KKO15_000703"
                     /pseudo
     CDS             complement(35076..35546)
                     /locus_tag="KKO15_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(35730..36524)
                     /gene="thyA"
                     /locus_tag="KKO15_000704"
     CDS             complement(35730..36524)
                     /gene="thyA"
                     /locus_tag="KKO15_000704"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBD6359614.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(36531..37406)
                     /gene="lgt"
                     /locus_tag="KKO15_000705"
     CDS             complement(36531..37406)
                     /gene="lgt"
                     /locus_tag="KKO15_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBD6359615.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(37557..39803)
                     /gene="ptsP"
                     /locus_tag="KKO15_000706"
     CDS             complement(37557..39803)
                     /gene="ptsP"
                     /locus_tag="KKO15_000706"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6359616.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKTRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(39816..40346)
                     /gene="rppH"
                     /locus_tag="KKO15_000707"
     CDS             complement(39816..40346)
                     /gene="rppH"
                     /locus_tag="KKO15_000707"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBD6359617.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41031..41720
                     /gene="mutH"
                     /locus_tag="KKO15_000708"
     CDS             41031..41720
                     /gene="mutH"
                     /locus_tag="KKO15_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBD6359618.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            41789..42502
                     /locus_tag="KKO15_000709"
     CDS             41789..42502
                     /locus_tag="KKO15_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD6359619.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            42640..42858
                     /gene="ygdR"
                     /locus_tag="KKO15_000710"
     CDS             42640..42858
                     /gene="ygdR"
                     /locus_tag="KKO15_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBD6359620.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            42966..44006
                     /locus_tag="KKO15_000711"
     CDS             42966..44006
                     /locus_tag="KKO15_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBD6359621.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44039..45229)
                     /gene="lplT"
                     /locus_tag="KKO15_000712"
     CDS             complement(44039..45229)
                     /gene="lplT"
                     /locus_tag="KKO15_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBD6359622.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45222..47381)
                     /gene="aas"
                     /locus_tag="KKO15_000713"
     CDS             complement(45222..47381)
                     /gene="aas"
                     /locus_tag="KKO15_000713"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBD6359623.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTCDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            47967..48998
                     /gene="galR"
                     /locus_tag="KKO15_000714"
     CDS             47967..48998
                     /gene="galR"
                     /locus_tag="KKO15_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBD6359624.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49005..50267)
                     /gene="lysA"
                     /locus_tag="KKO15_000715"
     CDS             complement(49005..50267)
                     /gene="lysA"
                     /locus_tag="KKO15_000715"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBD6359625.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50389..51324
                     /gene="lysR"
                     /locus_tag="KKO15_000716"
     CDS             50389..51324
                     /gene="lysR"
                     /locus_tag="KKO15_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBD6359626.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51311..52003)
                     /gene="ygeA"
                     /locus_tag="KKO15_000717"
     CDS             complement(51311..52003)
                     /gene="ygeA"
                     /locus_tag="KKO15_000717"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD6359627.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52132..53550)
                     /gene="araE"
                     /locus_tag="KKO15_000718"
     CDS             complement(52132..53550)
                     /gene="araE"
                     /locus_tag="KKO15_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBD6359628.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(53865..54626)
                     /gene="kduD"
                     /locus_tag="KKO15_000719"
     CDS             complement(53865..54626)
                     /gene="kduD"
                     /locus_tag="KKO15_000719"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBD6359629.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(54656..55492)
                     /gene="kduI"
                     /locus_tag="KKO15_000720"
     CDS             complement(54656..55492)
                     /gene="kduI"
                     /locus_tag="KKO15_000720"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBD6359630.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(55779..56960)
                     /locus_tag="KKO15_000721"
     CDS             complement(55779..56960)
                     /locus_tag="KKO15_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBD6359631.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57215..58444
                     /locus_tag="KKO15_000722"
                     /pseudo
     CDS             57215..58444
                     /locus_tag="KKO15_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14562 a        15874 c        14341 g        14232 t
ORIGIN      
        1 gttgccgggt tttctcgttt ttggtcactt actcatcaac tcatttcatt tgttatatga
       61 atgtttctta ccaccctcac gcgacaaata tcatcacagt taatatgtca tacaatttat
      121 gttgcaacac aaacgtttcc ctattttcat aaaccgtatt ttatcgctat gaaaaagaaa
      181 ttatcgccat gattaactaa aagtattgat tttttcagtt caacctacat atattgcgcg
      241 ccccggaaga agtcagatgt cgtttaatgg gcaaatattg cccttaaatt ctcttttact
      301 tttgatttac agagtaaagc gttgggataa tctatcttcc aagcagatta ttgtatttgg
      361 gatcaagatc actgatagat acataacttg tgtgtatctt tccgccctca aattattacg
      421 gcggtaaatg attaagccat cgccgataga cagatttcat ttttacggtc aggcaccttc
      481 cctggctgaa ctggctaaaa gctgaattat ttgcattcct ccaggagaaa tagatggaaa
      541 cgactcaaac cagcacgatt gcgtcgaaag actctcgtag tgcctggcgc aagacagaca
      601 ccatgtggat gctgggcctt tacggcacgg caatcggcgc gggcgtgctg ttcctgccaa
      661 tcaacgccgg tgttggcggt atgatcccgc tgatcatcat ggctatcctt gcgttcccga
      721 tgacgttttt tgctcaccgc ggcctgactc gcttcgtact gtctggtaaa aacccgggcg
      781 aagacatcac cgaggttgta gaagaacact ttggtattgg cgcaggtaaa ctgattaccc
      841 tgctctactt cttcgctatc tacccgatcc tgctggttta tagcgtggca atcaccaata
      901 ccgttgaaag cttcatgtct caccagctgg gtatgacgcc accgccgcgt gcgattctgt
      961 cgctgatcct gatcgtgggt atgatgacca tcgttcgctt tggtgagcag atgatcgtta
     1021 aagcgatgag tattctggta ttcccgtttg ttggcgtact gatgctgctg gctctgtacc
     1081 tgatcccgca gtggaacggc gcagcactgg aaacgctgtc tctggacact gcatctgcaa
     1141 ccggaaacgg tctgtggatg accctgtggc tggcaattcc ggtaatggtg ttctcgttca
     1201 accactctcc gatcatctct tctttcgccg ttgcgaagcg tgaagagtac ggcgatatgg
     1261 cagaacagaa atgctcgaag atcctggcat tcgcacacat catgatggtg ctgaccgtaa
     1321 tgttcttcgt cttcagctgc gtactgagcc tgactccggt agacctggct gcggctaaag
     1381 agcagaacat ctcgattctg tcttacctgg ctaaccactt taacgcaccg gttatcgcgt
     1441 ggatggctcc gattatcgcg attatcgcta tcaccaaatc cttcctcggc cactacctgg
     1501 gcgcacgtga aggcttcaac ggtatggtga ttaaatctct gcgtggtaaa ggtaagtcta
     1561 tcgaaatcaa caagctgaac cgtatcactg cgctgttcat gctggtaacg acctggattg
     1621 ttgccaccct gaacccgagc atcctgggta tgattgaaac cctgggcggc ccaatcatcg
     1681 cgatgatcct gttcctgatg ccgatgtacg caattcagaa agtaccggca atgcgtaagt
     1741 acagcggtca catcagcaac gtattcgttg tcgtgatggg tctgattgca atctccgcaa
     1801 tcttctactc tctgttcagc taagtccttt cgcgccgctt tcgggcggcg cttcctccgt
     1861 tttaacgcga tgtatttcct atgattagcg tattcgatat tttcaaaatc ggcattggcc
     1921 cttccagttc tcataccgtt ggaccaatga aagcgggtaa acaatttacc gacgatctga
     1981 ttgcccgtaa cctgcttaaa gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct
     2041 ctctgactgg taaaggccac cacacagata tcgccattat tatgggcctg gcgggtaacc
     2101 tgccggatac cgtggatatc gattccatcc ccggttttat tcaggatgtg aatactcatg
     2161 gtcgcctgat gctggcaaac ggtcagcatg aagtggagtt cccggttgat cagtgcatga
     2221 acttccatgc agacaacctt tctctgcatg aaaacggtat gcgcattacc gcgctggcgg
     2281 gcgataaagt cgtttacagc cagacttact actctattgg cggtggcttt atcgttgatg
     2341 aagagcattt tggccagcag gatagcgcac cggttgaagt tccttatccg tacagttcag
     2401 cagccgatct gcaaaaacat tgtcaggaaa ccgggctgtc actctctggc ctgatgatga
     2461 aaaacgagct ggcgctgcac agtaaagaag agctggaaca gcacctggcg aacgtctggg
     2521 aagtcatgcg tggcggtatt gagcgcggta tttccaccga aggcgtgttg cctggcaaac
     2581 tgcgcgttcc acgccgtgct gcggcactac gccggatgct ggtcagccag gataaaacca
     2641 ccactgaccc gatggcggtt gttgactgga tcaacatgtt tgcactggca gtgaacgaag
     2701 agaacgctgc tggcggtcgc gtggtgactg cgccgactaa cggtgcgtgc gggattatcc
     2761 cggctgtgct ggcgtactac gataagttta tccgcgaagt gaacgctaac tcactggctc
     2821 gttacctgct ggtagccagc gccattggtt ctctttataa gatgaacgcg tcgatttctg
     2881 gtgccgaagt gggttgccag ggtgaagttg gcgtggcgtg ctctatggcg gcagctggtc
     2941 tggcagaact gttaggcgca agcccggcgc aggtttgcat cgcagcggaa atcgcgatgg
     3001 agcacaacct cggtctgact tgtgacccgg tcgcggggca ggttcaggta ccgtgcattg
     3061 agcgtaacgc cattgcagca gtgaaagcgg tgaacgccgc gcgtatggca ctgcgccgta
     3121 ccagcgaacc gcgcgtctgc ctcgataaag ttatcgaaac catgtacgaa acaggtaaag
     3181 acatgaacgc caagtaccgc gaaacctctc gcggcggcct ggcgatgaag atcgttgcct
     3241 gcgattaatc tctcccagag gcctcgtttt gcgaagcctc ttcctgtttt ctcaaccagc
     3301 catagcctgt tccggcatcg aatgttaccc ttatcgcctg atctttaagg gggttatcgt
     3361 ggctgttcat ttgcttattg tcgatgcact gaatcttatc cgtcgcattc atgccgttca
     3421 gggttcgccc tgtgtcgaaa cctgccagca tgcgctcgat cagctcatta tgcacagcca
     3481 gccaacccac gcggtcgccg tttttgatga tgaaaaccgc agtagcggct ggcgtcatca
     3541 gcgtttacca gattacaaag cggatcgacc gccaatgccg gaagagttgc acgacgagat
     3601 gcctgcatta cgcgccgcct ttgagcaacg cggcgtcccg tgctggtcag ccagcggcaa
     3661 cgaagccgat gacttagccg ccacactggc ggtcaaagtg acacaggccg ggcatcaggc
     3721 aacgattgtt tcgacagata aaggctactg tcagttactt tcaccgacat tacgtattcg
     3781 tgattacttc cagaaacgtt ggctggatgc gccatttatc gataaagaat ttggcgttca
     3841 accgcagcag ttgcccgatt actggggact tgcggggatc agcagttcaa aggtaccggg
     3901 tgttgcggga atcggaccaa aaagcgccac gcagctgctg gtcgagtttc agagtctgga
     3961 agggatatat gagaatctgg atgcggttgc cgaaaagtgg cgcaaaaaat tagaaaccca
     4021 taaagagatg gcgtttctgt gccgcgatat tgcccgctta caaaccgatt tgcatatcga
     4081 cggcaattta cagcaattgc ggttggtacg gtaacggcga gccggatacg ccgcaaatcg
     4141 tatccggcat tatcacatca gcgcatttac caggcggtat ggtaaagctc tacaatatcc
     4201 tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat catccagtgc cgcctgagcc
     4261 agtgccgaaa tgtcttcctt gcgtacacca acatcacgca aatgtggcgg aataccgaca
     4321 tcacggttga gggcaaacac cgcttcaaca gcggcattac gcgccccttc caggctcata
     4381 ccttccactt tcacgcccat aacgcgcgcg atatcgcggt acttctcacc ggtaaagtca
     4441 gcgttatagc gcatgacatg cggtaacagg atggcgttcg caacaccgtg tggagtgtta
     4501 taaaacgcgc ccagtggatg cgccatacca tgcaccaacc ctaacccaac attcgagaag
     4561 cccatacccg caacatactg cccgagcgcc atttcttctc cggcatcctt atcaccagca
     4621 accgatcctc gcagcgcccc agcaatgatt tcaatcgctt taatgtgcag tgcatcggtt
     4681 agtgcccacg cgccacgggt aatatacccc tcaatagcat gagtgagcgc atcaacaccc
     4741 gtcgcggctt tcagcgccgg aggcatacca tccatcatgt cagcatcaat aaacgccacc
     4801 tgcgggatat catgcggatc aacgcaaaca aacttgcgcc gtttttcttc gtcagtgatc
     4861 acgtagttaa tggtcacttc tgccgcagta cctgctgtgg tggggattgc cagaatcggt
     4921 acactgggtt tattggtcgg ggaaagccct tccaggctac gcacatcggc aaactccggg
     4981 ttgttgctga taatgccaat tgctttacaa gtatcctgtg gagaaccacc accaatagcg
     5041 atcaggtaat ccgcgccgct attctggaat acaccgagcc cttctttgac gacagtaatt
     5101 gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca gccctgcagc atccatctta
     5161 tcggtcactt tcgccaccac gccgcattgc accagcgttt tatcggtgac gatcagcgcc
     5221 ttctgataac cacggcgttt cacctcatcg gttaaagccc caacagcacc ccgaccaaac
     5281 catgccgttt cgttcagaat cattctgtta gccatcatcc ttctccttgt tgctttacga
     5341 aattactctt caatccgtaa cccataggtt ttgaatttct ccagcactac ggcaatctct
     5401 tcatcgctca gcactggcac cgggtccgta atcgccaggg tcgtcaggta aagttgcgcc
     5461 agcacttcaa cttcatgcgc cagccataac gctttttcca gattcacctc acaagcgata
     5521 agcccatgat gttgtaacaa agttgcctta cgatttttga gagccagcgc aacatgttca
     5581 gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag gaatagaatt accgccagcc
     5641 gccgcaatca tgtagtgaat agcggggatc gatcggttaa gaatggaaac tgccgtgcaa
     5701 tgaacggcat gattgtgaac aaccgcgttg gcatccggtc tgctttgata ggctgccata
     5761 tggaaacgcc attcgcttga ggggagcttt ccttcctcat gtttaccgtt gccatcaata
     5821 aagacaatat gcgactccgt cagtttttca tatggaatgc ctgtaggcgt aatcagcatc
     5881 ccatcctgat aacgtacact gacgttcccc gctgtcccct ggttcagtcc caggcgggtc
     5941 atttccaggc aagtgtcaat aatctgacga gcaagtttat ttcgttccat tagctacctc
     6001 tctctgattc aaaacagggc aataatgttg ttcctttcac actattgaat tagccgttta
     6061 attacccacc atcttcttcc tgattaacaa gaaagaaatt cacaagctta tattttgtga
     6121 cctagctcaa ctaatcacag taaataactg caagttctct ttttataacc ccgttaaaaa
     6181 tgaccgctct taaaaatatt tatcaaaacg gtcatttttc catttcccct cgccagaaat
     6241 gcccgttttc ggcacaaaca attaatacgg tcatctggtt tgtgtttttt atgatttatt
     6301 ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg tagtcacata aagtcacctt
     6361 ccagctaata agtgtgaccg ccgtcatatt acagagcgtt ttttatttga aaatgaatcc
     6421 atgagttcat ttcagacagg caaatattca ccgatatgaa gcccgaactc gctggttttg
     6481 cacttttgaa aacataaccg attacgtgct taagcttctg aacctaagag gatgctatgg
     6541 gaaacacatc aatacaaacg cagagttacc gtgcggtaga taaagatgca gggcaaagca
     6601 gaagttacat tattccattc gcgctgctgt gctcactgtt ttttctttgg gcggtagcca
     6661 ataaccttaa cgacatttta ttacctcaat tccagcaggc ttttacgctg acaaatttcc
     6721 aggctggcct gatccaatcg gccttttact ttggttattt cattatccca atccctgctg
     6781 ggatattgat gaaaaaactc agttataaag cagggattat taccggatta tttttatatg
     6841 ccttgggcgc tgcattattc tggcccgctg cagaaataat gaactacacc ttatttttag
     6901 ttggcctatt tattattgca gccggattag gttgtctgga aactgccgca aacccttttg
     6961 ttacggtatt agggccggaa agcagtagtc acttccgctt aaatcttgcg caaacattta
     7021 actcgtttgg cgcaattatc gcggttgtct ttgggcaaag tcttattttg tctaacgtgc
     7081 cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga gcaattgagt gcgtataaac
     7141 acagcctggt attatcggta cagacacctt atatgatcat cgtggctatc gtgttactgg
     7201 tcgccctgct gatcatgctg acgaaattcc cggcattgca gagtgataat cacagtgacg
     7261 ccaaacaagg atcgttctcc gcatcgcttt ctcgtctggc gcgtattcgt cactggcgct
     7321 gggcggtatt agcgcaattc tgctatgtcg gcgcacaaac ggcctgctgg agctatttga
     7381 ttcgctacgc tgtagaagaa attccaggta tgactgcagg ctttgccgct aactatttaa
     7441 ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg tacctggctc atcagtcgct
     7501 tcgcaccaca caaagtcctg gccgcctacg cattaatcgc tatggcactg tgcctgatct
     7561 cagccttcgc tggcggtcat gtgggcttaa tagccctgac tttatgcagc gcctttatgt
     7621 cgattcagta cccaacaatc ttctcgctgg gcattaagaa tctcggccag gacaccaaat
     7681 acggttcgtc cttcatcgtt atgaccatta ttggcggcgg tattgtcact ccggtcatgg
     7741 gttttgtcag tgacgcggcg ggcaacatcc ccactgctga actgatcccc gcactctgct
     7801 tcgcggtcat ctttatcttt gcccgtttcc gttctcaaac ggcaactaac tgaacatatt
     7861 ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc ttaccgaaaa ttggtatccg
     7921 cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg cttgaagaac aaacaatgaa
     7981 tatggcgaaa gctacggccg cactgctgac cgagaaactg cgccatgcct gcggagctgc
     8041 cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg gctgaagccg ctgcttgcga
     8101 agaaaaattc agcagccaga atgtaggcct caccattacg gtaacgcctt gctggtgcta
     8161 tggcagtgaa accatcgaca tggatccaac ccgcccgaaa gccatttggg gctttaacgg
     8221 cactgaacgc cccggcgctg tttacctggc agcggctctg gcagctcaca gccagaaagg
     8281 catcccggca ttctccattt acggtcatga cgttcaggat gccgatgaca catcgattcc
     8341 tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc ggtttggccg tcgccagcat
     8401 gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg ggtatcgccg gttctattgt
     8461 tgatcacaac ttctttgaat cctggctggg aatgaaagtc caggcggtgg atatgaccga
     8521 actgcgtcgc cgtatcgatc agaagattta cgacgaagcc gaactggaaa tggcactggc
     8581 ctgggctgat aaaaacttcc gctatggcga agatgaaaat aacaaacagt atcaacgcaa
     8641 tgccgagcaa agccgcgcag ttctgcgcga aagtttactg atggcgatgt gtatccgcga
     8701 catgatgcaa ggcaacagca aactggccga tattggtcgc gtggaagaat cacttggcta
     8761 caacgccatc gctgcgggct tccaggggca acgtcactgg accgatcaat atcccaatgg
     8821 tgacaccgcc gaagcgatcc tcaacagttc atttgactgg aatggcgtgc gcgaaccctt
     8881 tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca atgctaatgg gtcaccagct
     8941 caccggcacc gctcaggtat ttgccgatgt gcgtacctac tggtcaccag aagcaattga
     9001 gcgtgtaacg gggcataaac tggacggact ggcagaacac ggcatcatcc atttgatcaa
     9061 ctcaggttct gctgcgctgg acggttcctg taaacaacgc gacagcgaag gtaacccgac
     9121 gatgaagccg cactgggaaa tctctcagca agaggctgac gcttgcctcg ccgctaccga
     9181 atggtgcccg gcgatccacg aatacttccg tggcggcggt tactcttccc gcttccttac
     9241 cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc atcaaaggcc tgggaccggt
     9301 actgcaaatc gcggaaggct ggagcgtgga attgccgaag gatgtgcatg acatcctcaa
     9361 caaacgcacc aactcaacct ggccaaccac ctggtttgca ccgcgcctca ctggtaaagg
     9421 gccgtttacg gatgtgtatt cggtaatggc gaactggggt gccaaccacg gagttctgac
     9481 catcggccac gttggcgcag actttatcac ccttgcctcc atgctgcgta tcccggtatg
     9541 tatgcacaac gttgaagaga ccaaagtgta tcgtccttct gcctgggctg cgcacggcat
     9601 ggatattgaa ggccaggatt accgcgcttg ccagaactac ggtccgttgt acaagcgtta
     9661 atacattaca ccctgatgtg atgcccggtc gctccggcta ccgggcctga acaagcaaga
     9721 gtggttagcc ggataaacaa tgttatccgg ctatattgca ggagcgatta tgaaacaaga
     9781 agttatcctg gtactcgact gtggcgcgac caatgtcagg gccatcgcgg ttaatcggca
     9841 gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc gatatcgcga tggaaaacaa
     9901 cacctggcac cagtggtctt tagactgcca ttttgcaacg ctttgctgat tgctgtcggc
     9961 aaatcaatag tgaactgact gaatgccaca tccgcggtat cgccgtcacc acctttagtg
    10021 tggatggcgc tctggtagat aagcaaggca atctgctcta tccgatcatt agctggaaat
    10081 gtccgcgaac agcagcggtt atggacaata ttgaacggtt aatctccgca cagcggttgc
    10141 aggctatttc tggcgtcgga gcctttagtt tcaatacgtt atataagttg gtgtggttga
    10201 aagaaaatca tccacaactg ctggaacgcg cgcacgcctg gctcttcatt tcgtcgctga
    10261 ttaaccaccg tttaaccagc gaattcacta ctgatatcac gatggccgga accagccaga
    10321 tgctggatat ccagcaacgc gatttcagtc cgcaaatttt acaagccacc ggtattccac
    10381 gccgactctt ccctcgtctg gtggaagcgg gtgaacagat tggtacgcta cagaacaacg
    10441 ccgcagcaat gctcggctta cccgttggca taccggtgat ttccgcaggt cacgataccc
    10501 agttcgccct ttttggcgct ggtgccgaac aaaatgaacc cgtgctctcc tccggtacat
    10561 gggaaatttt aatggttcgc agcgcctagg ttgatacttc gctgttaagt cagtacgccg
    10621 ttaccgtaat gcgctttcac tacaagcccc gtgtcctgac atcatccgca tcaatcgttt
    10681 tgcgttttat gaacgggcgc aaaaagcctt tgcgatcgtt atcacaggcg aacgagcgaa
    10741 gtacgggaat attcttttaa aaaaaggggt aacaccgtaa tctcataccg gtacgcccgc
    10801 atgacgcggg cggttatcga atgatggggt gaaaaatatg aaagcggcac gccagcaagc
    10861 gatagtcgac ctgctgctga accataccag cctgaccacg gaagctctct ctgaacagct
    10921 aaaggtcagt aaagaaacca ttcgtcgcga tctcaatgaa ttacagacgc agggtaaaat
    10981 tctgcgcaat catggacgcg ctaaatatat ccaccgtcaa aatcaagaca gtggcgatcc
    11041 ctttcacatc aggctgaaaa gccattatgc gcataaagca gatatcgcgc gcgaggcgct
    11101 cgcgtggatt gaagaaggga tggtgatagc cttagacgcc agttcaactt gctggtatct
    11161 ggcacgccag ttgcctgaca tcaacattca ggtcttcacc aatagccatc cgatttgcca
    11221 tgaactcggt aaacgcgaac gcattcaact gatcagttcc ggcggcacac ttgagcgcaa
    11281 atatggctgt tacgtcaatc cctcgctgat ttcccaactt aaatcgctgg aaatcgatct
    11341 gtttattttt tcttgtgaag ggatcgatag cagcggcgca ctgtgggact ccaatgcgat
    11401 caacgctgat tacaaatcga tgctattaaa acgtgccgcg caatcgttgt tattgattga
    11461 taaaagtaaa tttaatcgtt caggggaagc ccgcatcggg catctggatg aggtaacgca
    11521 cattatttct gatgagcgcc aggttgcaac ttctttggta acagcctgac ggaaagggta
    11581 gcaggccgga gacgacgccc cggccttgcc tgttatcgct cgtcgcgacg accacccacc
    11641 gcagcccaga tgcggcggac gtgcaccgtc acttcttcgc gatcgtgata caactgccgc
    11701 gcctgaatct gagcatttat gccatgttca tcaagctgtg cctgaatata cgccagattg
    11761 tgtgatactt cttcgtagcg ttttttcatc ggcagtttga gattgaaaat ggtttcacgg
    11821 caccagccat taaccagcca ctgcgccatc aatgccgcaa ctttagccgg tttttcaacc
    11881 atatcgcata ccatccagga gatattgctg cgtgtcggac ggaatttgaa accgtcttcc
    11941 cgcagccacg tcacctgccc ggtatccatc agactttgcg ccatcgggcc gttgtcgacg
    12001 gaataaaccc acatgttgcg cttcaccagt tggtaggtcc agccgccagg gcaagcgcct
    12061 aaatccaccg cccacatccc gttcgccagg cgttcatccc actcatccgc aggaataaac
    12121 acatgaaatg cctcttccag tttgagcgtg gaacgactcg gcgcctctgc cggaaatttc
    12181 aggcgcggaa tgcccatata gaacggcgaa ttattgttgc tgtatgagta accggtatag
    12241 cagcatcctg gtgcaataaa gaatacatgc acaaccggac gcttcggcgt ttcatagttc
    12301 gccagcaccc cagcatcgcg caaggcagcg cgtagcggaa cagtgaactt acggcagaat
    12361 ttcagcaact ctttgctttc gttggtatcg gcaacttcaa cacgcagttc accgcccttc
    12421 tctactacac cctgtaacat gccgacaatg ggggtaatac gatcttctgg cggcaaatgc
    12481 tgcaggagtt cccccaccac aaaccactgg cgggcaaaaa ttaatgaact gaacggcagc
    12541 tcacggatta acttatcgcc atcatcaggt tgataacatt cataaatgac ataacccgca
    12601 ttctctttca cgcgggcaaa accgaaaatt tcccgctggc cggctttatc ggtaatttct
    12661 gcggcgcact ctttttcaaa gcccggacgg cacagcaata caaccttatt catgacttac
    12721 gcccttgcgc tttaaacgga tagcaccaac taacattaac gcccagcccg caaggaagct
    12781 cacgccgcca accggtgtga caaacgccca caaacgcaga tgggacagcg ccaggcaata
    12841 aaggctgccg ctgaacagca ccgtgcctaa cgcgaggaaa acgctactcc agtaaaacca
    12901 gatactgatg cgacgctgca ttgccaccgc cagacctaag atcgccagcg tatgaaacgc
    12961 ctggtattcg agaccggttt ggatccagcc catctcaacg gcccccatgg ttttacttaa
    13021 cacatgcgca ccaaaagcgc ccagtgccac aaaaatgaag ccgctaatag cggcgaaaat
    13081 cagcataaaa cggctggtca tggtcgtacc ctacgtaaat tattgttcat aacgaaagcg
    13141 gaatttttct tgttcagcag cggctttcgc caggatccat tggcgaaagg cggctatttt
    13201 acccagttct gcctggctgt catgacaaac cagataaaaa gcatttttac tgaccagaac
    13261 atcattaaac gggcaaacaa gacgtccggc ctcgatttca gattgcgcca tcacgttatt
    13321 tgccagcgcc actccctgcc cgtgaatagc cgcttgcagc accatggcgc tatgactaaa
    13381 aattggccct tgctgaacgt tgatatgatt aaaccccaac tgtcgggtat atgtctgcca
    13441 gtcacggcgt gacgcatcat gtaataacgt atgtttagcc agatcttccg gggtcttcaa
    13501 gggtttttcg cctgtcagca gtagcggcga acacaccggc aataaatatt cggcgtacag
    13561 tttttccacc cgtagccccg gccagttgcc ccgaccataa aatatcgcca catcaacatc
    13621 atccgccagc ttatcttcct gacgatcaac cgcctggatt cgaacgtcaa ttcccggata
    13681 agctgaatta aagctggaaa gtcgcggaac caaccaatga atggcgaaac tggggagtaa
    13741 actgaccgtc aacgccccct tggcgctacg ggcctggagt ttacgcgtcg cttcggttaa
    13801 ttgcgaaaat atctctttga tatcgaggaa atagctttgc ccttcctcgg tcagcaggag
    13861 tgaacgatta cggcggcgga acagttttag ccccaaaaaa tcctcaagag acttgatttg
    13921 atgacttact gcggcctggg tcacaaaaag ctcttctgct gcgcgagtaa aacttaaatg
    13981 gcgtgctgcg gcatcaaaaa ctcgtaaggc atttagcggt ggtaatcgtt tagacatggc
    14041 ttttaaactt tgatgttaaa tgaatttaac aattagatca cactgtgtaa cctattagtt
    14101 tttttaatct gagccattat aaattgtccg ttgagcttct accagcaaat acctatagtg
    14161 gcggcacttc ctgagccgga acgaaaagtt ttatcggaat gcgtattctg gtgaactttt
    14221 ggcttacggt tgtgatgttg tgttgttgtg tttgcaattg gtctgcgatt cagaccatgg
    14281 tagcaaagct accttttttc acttcctgta catttaccct gtctgtccat agtgattaat
    14341 gtagcaccgc ctaattgcgg tgcttttttt taccttgcga tcgcgaatta ctgttccagt
    14401 tcgaccatct ctttcacgtc agtacggttg atctgctgtt tgttgccatt agcgtcttta
    14461 tacgaaatca taccgatatc gttatcagtc tgtggtttgc cgtcagagac gatggtacgt
    14521 ccgtcattgg tgtgcatcac atagttcgaa ccggaacagg cgctcagggc aaaagtcagc
    14581 atacaggcag aaataattgc ggcagtcttt ttcatcatct tctccttaaa acgattaata
    14641 aatcttaata gtattgatta acaggctaaa attaacgcct aacactattc agcatatgtt
    14701 acttacgcgg cttcgccagg atatccagat aattctgatg gttagcactc tccttgtatc
    14761 aaagtgaatt ttgcgtcacg atcggtgcat caagccgagg agtaccatga acgtttttaa
    14821 tcccgcgcag tttcgcgccc agtttcccgc actacaggat gcgggcgtct atctcgacag
    14881 cgccgcgacc gcgcttaaac ctgaagccgt ggttgaagcc actcgacagt tttacagcct
    14941 gagcgccgga aacgtccatc gcagccagtt tgccgaagcc caacgcctga ccgcgcgtta
    15001 tgaagctgca cgagagaaag tggcgcaatt actgaatgca ccggatgata aaactatcgt
    15061 ctggacgcgc ggcaccactg aatccatcaa catggtggca caatgctatg cgcgtccgcg
    15121 tctgcaaccg ggcgatgaga ttattgtcag cgtggcagaa caccacgcta acctcgtccc
    15181 ctggctgatg gtcgcccaac aaactagagc caaagtggtg aaattgccgc ttaatgcgca
    15241 gcgactgccg gatgtcgatt tgttgccaga gctgattact ccccgtagtc ggattctggc
    15301 gttgggtcag atgtcgaacg tgactggcgg ttgcccggat ctggcgcgag cgattacctt
    15361 tgctcattca gccgggatgg tggtgatggt tgatggtgct cagggggcgg tgcatttccc
    15421 cgcggatgtt cagcaactgg atatcgattt ctatgctttc tctggtcaca aactgtatgg
    15481 cccgacgggt atcggcgtgc tgtatggcaa atcagaactg ctggaagcga tgtcgccctg
    15541 gctgggtggc ggcaaaatga ttcacgaagt gagttttgac ggtttcacca ctcaatctgc
    15601 gccgtggaaa ctggaagcag gaacgccaaa tgtcgctggc gtcataggat taagcgcggc
    15661 gctggaatgg ctggcagatt acgatatcaa ccaggccgaa agctggagcc gtagcttagc
    15721 aacgcttgca gaagaagcgc tggcgaaacg tccaggcttt cgttcattcc gctgccagga
    15781 ttccagcctg ctggcctttg attttgctgg cgttcatcac agcgatatgg tgacgctact
    15841 ggcggagtac ggtattgcct tgcgggccgg gcaacattgc gctcagccgc tactggcaga
    15901 attaggcgta accggcacac tgcgcgcctc atttgcgcca tataatacaa agagtgatgt
    15961 ggatgcgctg gtgaatgccg ttgaccgcgc gctggaatta ttggtggatt aatgacaaac
    16021 ccgcaattcg ccggacatcc gttcggcaca accgtaaccg cagaaacgtt acgcaatacc
    16081 ttcgcaccgt tgtcgcaatg ggaagataaa tatcgccagt tgatcatgct ggggaaacag
    16141 cttccggctt tgccagacga gttaaaagcg caggctaaag agattgccgg atgcgaaaac
    16201 cgcgtctggc tgggatatac cgtggctgaa aacggcaaaa tgcacttctt tggcaacagc
    16261 gaagggcgca ttgtgcgcgg cctgttggcg gtgttgttga ctgccgttga ggggaaaacc
    16321 gccgccgaat tacaggcaca gtcgccgctg gcattgtttg atgagctggg attacgtgcg
    16381 cagcttagcg cctcacgcag ccaggggtta aatgcgttaa gcgaggcgat tatcgctgcg
    16441 acgaagcagg tttaagcaca gaaccgggtc ggataagacg ttcgcgtctc atccgacctg
    16501 attgtttaac cctgacgcgc cgctttcgcc atcatcttct tcagcgcatg agaaaccgca
    16561 acaaacccaa aggtggcggt caccatcgtt gccgcgccaa atccagatgc acaatccatc
    16621 ctcttcggcc cttctgccgt ggctttcatc gcacacaccg taccgtctga ctgcgggtac
    16681 accagcgctt cagtagaaaa cacgcaatcc acgccgagct tacctttact gtttttcact
    16741 acgccaaaat cgcttttcag gcgctcgcgc aacttcgccg ccagcggatc ctgaatcgtt
    16801 ttcgccagat cggtaacctg aatctgcgtc gggtcaatct gcccacccgc gccaccggtc
    16861 gtgaccagcg ggattttatt gcgccgacaa taagcaatca gcgccgcttt gggccgtaca
    16921 ctatcaatgg catcaatcac gtacgaataa ccggcactca tatattgcgc tacgttatcc
    16981 ggcgtcacga aatcatccac caccgttaca cggcactccg ggttaatctg gcgaatgcgc
    17041 tccgccatga cttccgcttt tgccagcccg acgttatcgc gcagagcgtg aatttgccga
    17101 ttggtattgg tgacgcacac atcatccata tcgataagcg tgattgcgcc aatccccgtg
    17161 cgcgccagcg cttccgccgc ccaggaaccg acaccaccga taccaaccac acaaatatgc
    17221 gcgtcagcaa acagttgcaa cgctttttca ccatacagac gcgctgtgcc accaaaacgc
    17281 tgacgccatg catcactaat taccacagac atagcacctc agaattaaaa agggcgaggt
    17341 tatccctcac cctaaacgca taaatgcctg atgcgctacg cttatcaggc ctacataact
    17401 cctgcaatat tttggatttg catgatttgt aggccggata aggcgttcac gccgcatccg
    17461 gtatgaacaa cgcgcactta gtcaataatc aggatgtgag gttacccctc accctgtcat
    17521 atccgtaaaa acggcataca gaatatcaca tcagccgcta aagacgttac ctgcgcccgg
    17581 agccgttttc agcacccaaa cccgtccata gtggttgtac caacctgcgc ggtgtccggc
    17641 ttccggcccg atcccttgat agatatcgaa gtgttggcct ttgattgcgc ccccgacatc
    17701 caacgccacc atcagacgca attcgtactg accgttaaat ttgccgttat tatccagcag
    17761 cggcacttct gccagcaagg tagtacctgg cggaataatt gaacgatcgg aggcaacaga
    17821 agcacggcca accagcggca ccgcacttgc ccctttcacc ggtgcaaaag attgcggttt
    17881 aaagaagacg aaagacgggt tctgttccag cagctcgcga acctcggctt cactgtgtgt
    17941 ttcgccccaa tgacgaatcg cctgcatcga catatcttct tttttcactt cgccacggtc
    18001 gatcagcacc ttaccaatgc tgcgataggc atgaccgttt ttccctgcat agctgaaaaa
    18061 gttaagcgga ctgccatcac caaagtcgat atacccacta ccctgcacat ccataatgaa
    18121 gttatccatc agagagttgc tgtaagcgag gatgtattta tcgcccaatg ccccggcgta
    18181 gatctccgca cgagacggca gacgaccacg ttttggcggc atacggtaaa taggatactg
    18241 gaactcgccc tggcgggtat ggcgcgcctg aattaccggc gtgtaatagc ctgtaaactg
    18301 cacgttacca tagttgtcgg caccttccat ctgccaggca tcaatgccga actggcgcat
    18361 attgcgggta tcaccgcctg cgcgcagcca ctcttgcacc gcgttataaa cattactctg
    18421 gttgccatac agacgcggtg acgaattacg gatatggtta atttgctcgg caaaatcacc
    18481 ggcgttaatc ggcgcgccaa cggcatctgg ctggttcacc agagagaaag gctgggtaaa
    18541 tttcccgtct ttatattgct gtccgcgatc ggttggtttg gaagagcagg cggcaagcat
    18601 tgccacaacc gtgcccataa gaaggtactt tacccaacgt cctttcattg ttctcttctt
    18661 caggttaaaa aataaggcgc aacgaagata acaaaccgcc gcgtttaatg aaatgagcag
    18721 cctctccctg acgcaaattt tgcacaaaaa ataggcttta gtggtttgtt tttgttcaaa
    18781 atcatgctaa atccgtgatc ggggtaaaaa aaaggttgca tgaaaacgcg agcggagtat
    18841 agtgcgcatc cacggacgcg gggtggagca gcctggtagc tcgtcgggct cataacccga
    18901 aggtcgtcgg ttcaaatccg gcccccgcaa ccaatcaaat ttgatgaagt aaaagcagta
    18961 cggtgacgcg gggtggagca gcctggtagc tcgtcgggct cataacccga aggtcgtcgg
    19021 ttcaaatccg gcccccgcaa ccaattattg aacaccctaa cgggtgtttt tttgtttctg
    19081 atctcccata aaaaagcgcc attcagcgcc tttttatcat ccccttctcg ccagcgttgc
    19141 cccatcggca aaatacgctt taatccccgc aagaatagac tccgcaactt cctgctggaa
    19201 agtcgccgtt ttcagtttac gctcttcctc aacgttactg ataaacgccg tttcgaccag
    19261 aatggaggga atatctggtg cctttagtac ggcaaacccg gcctgttcaa cttgattttt
    19321 atgcagcttg ttgatttttc cgagcttatt cagtaccgct ttaccaaact taaggctgtc
    19381 agcaatagtc agcgactgca ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct
    19441 tttgctcacg ccaccaatca agtccgaggc gttctgggtt tgtgccagat attttgccgc
    19501 agtactggtt gcgcctttgg ttgagagcgc aaacaccgag gaaccgctcg gctgacgact
    19561 ggtaaaggcg tcggcatgga tagagacaaa caggtcagca cgctgtttct gggcttttgc
    19621 tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt
    19681 gccctcttcc tcgatcagag agcgcaaacg gcgagctatt tgcaatacca catctttttc
    19741 gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat
    19801 aatgacaatc ggacgatcgc gccctgcttt acccggttgt ggaccacttt gcgctggcgg
    19861 cacctgcttt tcgaggtcgc ctttgttgta atcctccagc agcgccagca gcgggtcctg
    19921 catatcctgt gcattggccg gatagaggtc catcaccaga cgctctttaa acccggcgac
    19981 cggcgcaagg gcaaacagct gcggttttac gttttgcttt aattcaaaaa ccatgcgtac
    20041 ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat
    20101 ttgtgccgcc atccccttga gtaccgagtt caggtttaca tcttcgatat ccaccaccac
    20161 gcgttcaggg ttactcaacg cgaactgctt atatttcagc tgacgatttg attctaccgt
    20221 cacgcgggtg tagctggacg caggccagac gcgcaccgcc acgacctggc taaccgcagc
    20281 caggctgacc tgacttacgc tcaatagcca catagcgccc gcgccttgca gtaaacgacg
    20341 acggctgatt gcagtgttgg atcctgacat gcctctcccg agcaaaagaa atctaagctg
    20401 tgtaacaagt aaacgactaa tttgaccgga aactttagcg aaagacgcat aatctgtcat
    20461 ctaataaacg gtaaacattc tttttatatt cacggcatta ctgataaaaa agtcgctctc
    20521 gcataaaatt tacacttgca ccctgcgaaa aaacagaata aaaatacaat aatttcaaat
    20581 aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc
    20641 cgccattcgg ttccctatat caatacccac cggggaaaaa cgtttgtcat catgctcggc
    20701 ggtgaagcca ttgagcatga gaatttctcc agtatcgtta atgatatcgg gttgttgcac
    20761 agcctcggca tccgtctggt ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg
    20821 gctgcgcatc accacgaacc gctgtatcac aagaatatac gtgtgaccga cgccaaaaca
    20881 ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg atattactgc tcgcctgtca
    20941 atgagtctca ataacacgcc gctgcagggc gcgcatatca acgtcgttag tggcaatttt
    21001 attattgccc agccgctggg cgtcgatgac ggcgtggatt actgccatag cgggcgtatc
    21061 cggcggattg atgaagacgc gctccatcgt caactggaaa gcggtgcaat agtgctaatg
    21121 gggccggtcg ctgtttcagt cactggcgag agctttaatc tgacctcgga agagattgcc
    21181 actcaactag ccatcaaact gaaagctgaa aagatgattg gtttttgctc ttcccagggc
    21241 gttactaatg acgacggtga tattgtctcc gaacttttcc ctaacgaagc gcaagcacgg
    21301 gtagaagccc aggaagagaa aggcgattac aactccggta cggtgcgctt tttgcgtggc
    21361 gcagtgaaag cctgccgcag cggcgtgcgc cgctgtcatt taatcagtta tcaggaagat
    21421 ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg gtacgcagat tgtgatggaa
    21481 agcgccgagc agattcgtcg cgcaacaatc aacgatattg gcggtattct ggagttgatt
    21541 cgcccgctgg agcagcaagg tattctggtg cgccgttctc gcgagcagct ggagatggaa
    21601 atcgacaaat tcaccattat tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat
    21661 ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc
    21721 agttcatcac ggggcgaggt tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc
    21781 ttaagcaaat tgtttgtgct gaccacgcgc agtattcact ggttccagga acgtggattt
    21841 accccggtgg atattgattt actgcccgag agcaaaaagc agttgtacaa ctaccagcgt
    21901 aaatccaaag tgttgatggc ggatttaggg taagcgatga aaatcaccgg atgcggcagg
    21961 cgtaacactc gtgcgtcgca tccggcaatt acgtttattc ccgcgaacta aacaacgccg
    22021 ccagaccact gcgccgctca gtacgagtgg cgattgccgc acttaatata cgctcatcgg
    22081 catacagcga cagacgacga cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta
    22141 ctaccggcgt gcgttggctc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg
    22201 atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg caagcgactc ggttgcacag
    22261 acttaatatt gccgtccggc atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg
    22321 caataccgat atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg
    22381 gtcggccttc gtaccaacga gagtgcggat gacgatgaat tttgcgcttc tgttgcataa
    22441 actgttcaat tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca
    22501 aaagctggta ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca
    22561 gatccagata acgtccgtaa cccgcaagag cttcctcaag catcgcaata taatcttcgc
    22621 cgctctgtaa aagtcgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg
    22681 ccgttttatc accacggttg atcgccgcag ctaactgacc aatgccagaa tcgctgccga
    22741 aacgatagct tttttgcagc aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg
    22801 ttcctgccgg aacgtgagtc cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa
    22861 agcccgcgtt ggcataagcg cagatatcgc ccagcacagc cccagcctca accgaggcca
    22921 gttgatcacg atcgccgaga aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc
    22981 tcgacatcat aggcagatcg atcattgacg cttcatctac caccagcaca tcaagatgca
    23041 gcgggttacc ggcatgatga cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt
    23101 gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc
    23161 gcaaagcctt gccgagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca
    23221 gacggatacg gcagcgttcg ccgtcggcca tttgaattaa cgctgccagc aacttcgcca
    23281 cggtggtcgt tttaccggtg cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg
    23341 ccactgccgc tgcaactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca
    23401 gggtttgcgc cagtagagct tcatcaacct caatggtatg attcacctca ttgaagaagc
    23461 gcgctaccgt ccgctcgtta caccacatgc gattcaaata aagacgatcg ccacagagga
    23521 tcatcggcgt gggttcatct cccctgctga ccgcttgaga agccagcaag cattcttccc
    23581 aattttgtag ctcaccgatt tcactgacac aggtcgccaa cagcggattc gacgcctcgt
    23641 tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc tccggcatca tgacttaaca
    23701 gtgccgccgc gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaattgca
    23761 catccagcgg gcgtagctgt ttgtgctcca cagcttccag taattgcttt tgcaatttca
    23821 ttacgcctcc tccagggtca taccggcaaa catctcatcc atcagggcaa tcaacccggc
    23881 gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct ttatcaacgc cacgcaggaa
    23941 cagatagata acgccgccaa agtgaagatc atagtcgtaa tcagcaatgc gatggcgcag
    24001 ataacgatgc agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat
    24061 cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct tcacccagcc agttggattt
    24121 atagtcgagc aggtaataac gcccttcgtg gcggaatacc aggtcgataa agccttttaa
    24181 catgccgcgt acctgcatga actccagcgg cgggcagcca gcggatagcg ggtcaaactg
    24241 gcgaattagt gcatcaagct ggctggcgat gagcggttcg ctaatcggca ggtaaaactc
    24301 catctccacc tgtttatcgc ggtcggaaag ctgactcagg ctaacacccg tttcattgag
    24361 aggtgcctgg aggacagccg tgatccactc ggtcaacacc ggttcccact gcgattcaaa
    24421 gccgccgagt tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag
    24481 gtcttcaaac aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg
    24541 tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg
    24601 catcagatcc tgagcgatac cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg
    24661 ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc agttctgcag ttaaagcatc
    24721 attaacctgc cagggctggt tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc
    24781 gcataacgct tcaatacagg tgcgaagccc tgccgcatct tgcggttccc ctttttgcag
    24841 caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg
    24901 caccagcggt gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag
    24961 caaacgcaga tcttccgcca gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc
    25021 attaagatcc aaaactgcct caaacgagtg gcgatcgtga taaaacgcct gatcctggac
    25081 gcggaaattg gtaataaatg gcaaccagac cagcggatat tccagccctt tcgatttatg
    25141 gatcgtgaca atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc
    25201 attactgtct ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc
    25261 cagctgcgtt ccggcttctt gaagcagttc gctgatatgc aagatatcgg taagacgccg
    25321 ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg
    25381 cagcatcggc ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac
    25441 taccgtatcc cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc
    25501 catcattgac gttgccagcg cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc
    25561 ctgcaacaac caaagcattt cctgcgcttc cagagtttca aaaacactgt cgcggttcga
    25621 aaggtaaacg gaagggattt ccagcaacgt taaggcatcg cgcacctggg cggcctcctg
    25681 gcggctgcgc accagcacgc tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt
    25741 catcagcaac gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca
    25801 tacctgcgcc atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca
    25861 cattttcatc gcaggctgcg tttcaccttt aaatacaaaa cgtaacgcct gatttttccc
    25921 ggctgatttc actggaataa acggtatttc gcgaaacatg aacgtgtcat cagtctggct
    25981 gaaaagcttg ttcacactgt tcaccattcc aggtgcggag cgccagttgg tgtctaaagt
    26041 gtagtgggcg tgaacttcac tacgcgcctt catataagtg aagatatccg caccccggaa
    26101 tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg
    26161 ccagatacgg cgaaaaattc ggtactgctg ggggtcggta tcctgaaatt catcgatcat
    26221 tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag
    26281 cgcggaatcg agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc
    26341 acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg atcaacagat cgcggatcga
    26401 caatggttct gcaagcagtt gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc
    26461 cccggccttc gtgcgatctt ctaagaaacg ctgggagaat ttttccagcg actccggcaa
    26521 ctgataactg tttgtctctt cttctgccca ggcgctgatc ttttcgatcc atttagcctg
    26581 attgctacgg ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc
    26641 acccactgcg tcgcgccact gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg
    26701 gcgggaagcc agcgtttcat catcgggcgg cggtgctttg ataaccggcg gttcgccttg
    26761 cagataacga ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagacgac
    26821 ctgggctatt tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg
    26881 gtagcgtagc agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa
    26941 ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc
    27001 catctgccgt tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcttc
    27061 cagcaggcgt tcgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc
    27121 gtggatattg ctacggatac gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac
    27181 caccagcagt tcttcaacga tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag
    27241 taacaggcgc aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc
    27301 aatcaggcgc tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact
    27361 cattcttttc actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt
    27421 tccagccttc aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca
    27481 gaatagccat gccttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg
    27541 gcgtcagcag ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc
    27601 gatcgaaatc tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca
    27661 tctgggtaat taccgcctgc tggatttgcg caaactcatc tggcttcatc gctcgcaatt
    27721 ttgcctctgc ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat
    27781 cattgctttg caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa
    27841 acacggcata gcccaattgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga
    27901 tctgccccaa cagagagcta taggctgagc tggtgtattc atcgtagcca gtcggtacaa
    27961 ataccgctgc cagtgcggag tcggtgctgt taccggcttt ttcaaagatg acggattgtt
    28021 ttttatcgac cactacatct ttgtttcgac accactctga accatcagcg cccaactgtt
    28081 tttgcacatc gcgtgccagc gttgttgcct gggcctcggt catgttgccg ataaccataa
    28141 actctggtcg agcccctgat tttaaggcgt cgcgataggc cagcacctct ttcaacgtaa
    28201 tggagggcaa aattttacgc cgttcatctc gcgagaagta cggcacttgc gagagcatct
    28261 gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc tgcggaatcc atcatctggt
    28321 tataccagga cttcgcctgc tcaagctgct cttccgtagc ggtatagcta aagtacccct
    28381 cgagcaatgc ctggaacagc tgcggcagac gctgggtgta accattagca ttaatcataa
    28441 ggccgttgtt agcgttggtg gaaaaactta tgccaccaac cgacgcctgg ttgcttaact
    28501 gatcaagcgc cagccctgcg agataatcat tgagcgcaaa catcacctga ttgcgggcgc
    28561 tgtccatggc tttcggatta cgcaaaatca ggctgacatc agctttgggc tcgctggcaa
    28621 aataacggct tggcgcatac accacgcgca gattcgactc atcaacaatc agctctggat
    28681 ggtcgtattt cttctctgac ttaatcagcg agaaatcatc aggaatataa gggttaagct
    28741 ctggcaaaga gagcgcaatg tcggcggctt ttttctgcca gtcggcgaaa gtttgtgcgc
    28801 tgattttatc gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt
    28861 tcgggctgat ataccagata cgcgcattct gcggcgtcat catcgccaga cgttccttta
    28921 ctgctttagc atcgtaccga tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa
    28981 cgcgaatcat ggtatctgcc agccattcga cgtaatccat atcacgggtg atcgacggat
    29041 aacggaagtc gatatccagc acattcgcca gttcatcgaa gtattgttta tcaatgcctt
    29101 tttcacgtaa cagattgaga tagctaaaaa ttgccgccac aacctgatcg cgattagcca
    29161 ggcctttatc agttaaagac gcagagatcg ctaatacgcc gctgttgccg ttgacgatag
    29221 gatcggagtt ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg
    29281 tacctgggct gcgattgcca atcagatagg taatcaattc atcggtttta ctacggaact
    29341 tcgctgagtt gttatcgatg cgaaactcaa cgcgcaacac tttacgcggc agcgcaggga
    29401 cgtaatgaat gataatgccc ttttgcgcgt cggtgactac cggcacggtg atttccggtt
    29461 ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc cgccattttt gccaactccg
    29521 gcagcggttt attactgtaa ataaccgcct tcatcaaatt ggcggaatag tacttctcgt
    29581 ggaaatcttt cagcgcctgc tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt
    29641 taccaccaga aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg
    29701 ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc agcgttcact gcattacgct
    29761 cacgttcggc gtatttcttg tcgagcaagg gttctgcaat agcatcggcc aggcggtcta
    29821 ccgcaccggg caaggcgtcg ttctcaactt ccagatagaa agccgtgcga tacggcgcag
    29881 tgctggcatt gtgactaccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg
    29941 ggtacttttt cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg
    30001 cctcgggatc ttccagcgac ccaacgggca ctaccagcgc cgagagcgat ttaactgcct
    30061 gcggatcaga aaccagcaag accaccatac cgttatccag acgtatagcc tgatactggc
    30121 ggttatcttt atcactttta cggatggttt cctgaatcgg ctgccatccc gtttctgcct
    30181 gacttaatgg tgcccagagg gcaacaaaca acaataatgc tttgaaccag gtgctgcggg
    30241 gcattcacgg acctcataat caactttatt ttctggacag attcaacagg ttaacgcaaa
    30301 tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc tatgtttata taaccatcag
    30361 tccgtgactg gtgcgcatca taaagtaagc ggatagattg cgcaattttt atacaacact
    30421 catgactgat taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatggcc
    30481 tccattgtct ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg
    30541 ccacgcacca tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac
    30601 gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg
    30661 ccgccacttt caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc
    30721 agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcaccg
    30781 tctttgcgta gaaaaaggcg actttcgcca ttaccaccgc tggcacagta gacaaggtgt
    30841 tccagccaaa gttgcattcc ctgcgccaca cttaataaag agggacgcca gcgcaacagg
    30901 ccatccggct gcacctgcgg caaccagcca gttatctgca caccgttgca ggcgagatca
    30961 atttccatac tctgccccgg ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc
    31021 tcctggcact gtgtttccca gaaaatttca ccaaaagcgc catacggtaa atcccctgcc
    31081 gcccggaagc ggcggaacaa gcgttcggca tcatcctgct caaccagtgc attcaataac
    31141 tgctgattga tttgataacg gctaagtcct tccagaataa atggctcggt gtctgggatt
    31201 tcgctgtctt cagtatggaa gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga
    31261 tgtgcccaga atcgttgtag cgtttccagc ggcacggttt ccggtaaggt aaacggcagc
    31321 ggctgaacaa attcagaatg tgctttacca gcctggctgg ccgcaggtag ccattcacga
    31381 gcatagcttt gtcgttcgcc tggctggtag ttttgcggat caaacggcat ccgggtatgg
    31441 aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg
    31501 cccggtagat aatgactttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg
    31561 aaacgttcac tgttatcctg aatggaacgc ccgatatagc tgatatagag tttttgctgc
    31621 gcggaaatta acgcttccag gaacaggtag cggtcgtcat cgcgacggct acggtcgcca
    31681 cgcttcggtt tctggctcat caggtcaaag cccaatggcg caagctgacg tggataaacg
    31741 ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga
    31801 gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga tacgttcttg atccagacgc
    31861 tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa
    31921 ccttcggcga taatcgcctg ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc
    31981 gcatccggca ggaagaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttctagc
    32041 ggacgctcct gtgccagccc gcgacgccag atgtttagct gcattagcag tgaagccaga
    32101 tgccccacca gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac
    32161 tcgccctgcg cgctctccat cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag
    32221 gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa
    32281 cgaatgccgg attcgttgac ccactggcgt aaataacgca gcccttcttc ggtgatgtca
    32341 aaccgcgccg ccagcaccgg cacatccagc agcgccagca catcctctga cacaaagcga
    32401 ctgtcaggca gtgataacag gctgataaac gcttccagta ccggatgtga ctgccgcgca
    32461 cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc
    32521 tgaataaacg gactgtagct gtcgatatca gccaccatca cgatgatgtc gcgcggagta
    32581 agtgtcgggg cttcctccag catcgccagc aagcgatcgt gtaaaacttc aacttcacgc
    32641 tgcgggctat ggcaaacgtg gaatgtgata ctgctatcca gtggatcaag cgggcgtttg
    32701 ttatcgctac gggaaaactc ttcgatgttc acacccgcaa cggcgcggtt ttccagttcc
    32761 agaatgtcag actgaatgtt atgcagcagg ttatctggcg tcacatcgac aaaagcgtcc
    32821 agctcctggc tgctctccag gtcagaaagg agataaatgt agtcgcgccc aagcttcccc
    32881 catgaggcca gcagcgagtt gccgacatcc tgttcaccat cgctgttaaa gagctgtccg
    32941 gcattttcgc tatcgcgaaa tagcggcaat tcgcgatctt caaaactgtg tcgacgctga
    33001 cgagtcagca gtttcgccag ataagcagga tctttaatat cgccccagta ataacggcag
    33061 gggttggtaa acaggagatg gatttcaata tgtttaccca gcgcctggag cgcctggaga
    33121 taaacaggcg gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg
    33181 caggtcgtcg cggactccag cgtttcgata aagcgctgat agagattcgc gcggtgccag
    33241 cgcggttgtc cgagttcatg ggtatattcc accagcgcct tccacaacgg ggcttgccag
    33301 gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc
    33361 ggacgataga ccagatactg gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt
    33421 tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc
    33481 agcaattgcg gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaggcgctc
    33541 tctttgggga tttccggtaa cacccggacg aacatatccc agataaagct cgctggcagc
    33601 ggaaaatcaa tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgc
    33661 gccataccgg tactttgcac cagaatcatc tctggttcga aaggatcgtc cagccgttcg
    33721 cgttcgacaa taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc
    33781 cttaacatag cggctcctga ctactgacga ttcgggcaat gcaggcgggt catctcgcct
    33841 tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc
    33901 tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc
    33961 caaccatgcc gccagagctg ttggtactgg tttctgctgg cgagactgtt cattgatgtt
    34021 cgctggatac ccgataacgc agtgacaatc atcaccatca gcaccatcag caccatcgca
    34081 accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataatgccc
    34141 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    34201 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcttcat
    34261 tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc gagcactgaa
    34321 ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac tgaacgatgg
    34381 cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    34441 gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    34501 acgaaacacc tttttcgcgg ttcaccggtt gaatcctgtc acgctatagc tggcatccac
    34561 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa
    34621 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    34681 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg
    34741 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    34801 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    34861 ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    34921 tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta aactttcacg
    34981 ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc
    35041 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    35101 tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    35161 tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    35221 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    35281 ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg
    35341 ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    35401 tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    35461 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    35521 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    35581 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    35641 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    35701 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aacgcccgga
    35761 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    35821 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    35881 tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    35941 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    36001 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    36061 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    36121 acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt
    36181 accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    36241 ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat ggtgacattg
    36301 ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag ttcgtggatg
    36361 atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg caggttaaaa
    36421 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    36481 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    36541 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    36601 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    36661 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    36721 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    36781 accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    36841 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    36901 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    36961 ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    37021 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    37081 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    37141 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    37201 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    37261 cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    37321 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    37381 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    37441 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    37501 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    37561 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatga
    37621 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    37681 aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    37741 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    37801 cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    37861 aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt gcgatcaacg
    37921 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    37981 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    38041 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    38101 gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag gttgcccgta
    38161 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    38221 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc
    38281 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    38341 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc
    38401 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc cagtttttct
    38461 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    38521 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    38581 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    38641 ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc gcccatcacg
    38701 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc
    38761 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    38821 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    38881 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    38941 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    39001 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    39061 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc ggcaaagcgt
    39121 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    39181 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    39241 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    39301 tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    39361 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    39421 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    39481 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    39541 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    39601 agcgttacag tgcgaccgcg tgtttttttc agcccccggg tcgccatcag gtagtaacaa
    39661 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    39721 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    39781 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    39841 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    39901 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    39961 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    40021 gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    40081 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    40141 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    40201 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    40261 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    40321 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    40381 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    40441 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    40501 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    40561 cccggagcat tctgaataag cccgatatag aaattttaaa atattcatta ttagtcaatg
    40621 cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    40681 ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    40741 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atgaaaacag
    40801 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    40861 ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc gattaccggc
    40921 agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa gttacactgc
    40981 gaatattcgg cacataatta ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    41041 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    41101 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccagagaatt
    41161 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    41221 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    41281 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggtaata
    41341 gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta ctgtggatac
    41401 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca ccgttgctgt
    41461 ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa ttaatggata
    41521 tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat ttacagataa
    41581 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga
    41641 ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    41701 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    41761 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    41821 ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    41881 tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    41941 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct cttgggttac
    42001 gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgatttgat
    42061 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    42121 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    42181 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    42241 agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc
    42301 cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc
    42361 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    42421 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acttgattcg
    42481 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    42541 cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    42601 cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    42661 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    42721 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    42781 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    42841 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    42901 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    42961 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    43021 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    43081 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    43141 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    43201 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    43261 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    43321 agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccgacca
    43381 actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt tcgctgctgg
    43441 atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    43501 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    43561 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    43621 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    43681 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    43741 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    43801 atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt gtacgccgtc
    43861 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    43921 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtacatc
    43981 aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc gttaaatatt
    44041 aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc gcaccgaagc
    44101 caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa atgcccagca
    44161 tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga
    44221 cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca
    44281 gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggagaaaa
    44341 tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg
    44401 taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc atcgcgttga
    44461 gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca
    44521 ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg
    44581 tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga
    44641 gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg gcaacgaccg
    44701 caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag tcagccagca
    44761 caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc attaaaccgt
    44821 tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg
    44881 aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc
    44941 agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg
    45001 cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca cctacaaaca
    45061 ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt aacgccagag
    45121 tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt
    45181 tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc
    45241 gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc
    45301 atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg
    45361 gcatactgtt gcaacttatc gcacgtcagt tcgttatctg tggtgaaaag caccagtgcc
    45421 tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca
    45481 agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt
    45541 tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg
    45601 tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca
    45661 cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt
    45721 ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg tagaatacgc
    45781 cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg
    45841 gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta
    45901 ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg
    45961 ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg
    46021 cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata aaggaacact
    46081 tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc
    46141 ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg
    46201 acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc ttcagaaccg
    46261 gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc caggcgcggc
    46321 atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct
    46381 tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta
    46441 tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc
    46501 agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg cgcgatggcc
    46561 ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct
    46621 tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga
    46681 taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac
    46741 attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat acgcgcttcc
    46801 atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca
    46861 tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg gaacaagcga
    46921 cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca
    46981 ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc
    47041 agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc
    47101 accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg aacaaagtcg
    47161 ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac
    47221 actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta
    47281 ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga
    47341 tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg
    47401 attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat
    47461 taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga
    47521 gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacgacg
    47581 actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt
    47641 gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg
    47701 gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct gggatcacca
    47761 ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg
    47821 taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc tcacattccc
    47881 acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact gaaacaatgt
    47941 aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag
    48001 gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagctt
    48061 cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc
    48121 gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat gtttccgatc
    48181 cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc ggtaatttct
    48241 tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt gagcaactga
    48301 tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccggat gccgatttag
    48361 cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggctttg
    48421 aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa
    48481 ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg
    48541 aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg gccaatgatc
    48601 ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg accgagcttt
    48661 tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggcg
    48721 cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct
    48781 ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa
    48841 tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat aatcgccctc
    48901 tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca gtgtcaactc
    48961 cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc
    49021 gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac
    49081 agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc gccatatgcc
    49141 cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc gcgggtttca
    49201 acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg cccggcgacg
    49261 acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag ggcactgata
    49321 tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc
    49381 acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa tacgccagac
    49441 tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc caaatggcga
    49501 gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct
    49561 tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga
    49621 ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg ggcataatca
    49681 acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg ttgtatcacg
    49741 tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta
    49801 ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca tacccgatgc
    49861 cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt caccggaatt
    49921 tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata
    49981 tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat ttcgcctaac
    50041 gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg aatattggaa
    50101 caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat ctgccgacga
    50161 ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa acgcagcaga
    50221 ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac aaactccaga
    50281 tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc
    50341 gagtctatgc aaaaatgata tggattaccg gattgcgaga gagcgctaat ggccgccgtt
    50401 aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggca
    50461 gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag
    50521 gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac cgtgcaagga
    50581 ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc
    50641 gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt
    50701 tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc
    50761 ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat
    50821 gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac cgaattactc
    50881 tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa aaaggtatta
    50941 acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga cagctatcgc
    51001 cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat cgtagaaacc
    51061 cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc ggtggttaac
    51121 ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt cagtattgcg
    51181 gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggttcag
    51241 gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct tgacgctatt
    51301 ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcggtat
    51361 caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct gtgcagccaa
    51421 aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca taataagcgc
    51481 gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga ttaattttcg
    51541 cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc agccgcccgc
    51601 gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga gtcattccgg
    51661 ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga gtgcaacgtg
    51721 actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata ccttctgcgc
    51781 ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta tcccattccc
    51841 cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc atttgcgcag
    51901 agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag taaggaatgg
    51961 tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc tcctgatgtg
    52021 tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc aggtgcccaa
    52081 tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa atcagacgcc
    52141 gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca gcgtgacatt
    52201 tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca gcgcagtgta
    52261 gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg ccgcgccgat
    52321 aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat cgcggcattt
    52381 cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca tcgcataacc
    52441 ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg aagccgtacc
    52501 gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca tcacgctgaa
    52561 accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg caatgaaggt
    52621 cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt
    52681 aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa
    52741 ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat
    52801 cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc
    52861 ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg cctcaatatg
    52921 acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca gaataatcag
    52981 cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa
    53041 cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact
    53101 gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta
    53161 agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca tctctacgct
    53221 ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct
    53281 gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc
    53341 gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa agtgatcggt
    53401 aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac
    53461 cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag aacgtggcgt
    53521 taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag taagacattg
    53581 tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata aatatggatt
    53641 aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta agttgaatta
    53701 ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag ttcattaatt
    53761 gataattgat atggattatt tcataaccat gatatggatt atgatgatct agagatataa
    53821 aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg cgccagccaa
    53881 ccgccatcca cagcaatggc ataaccattt acataatctg aagcgctgga ggcaagaaac
    53941 actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat gcgatcgaga
    54001 atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt cgccatgtaa
    54061 cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc catcaatcgc
    54121 gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg
    54181 aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc gataaagtgt
    54241 ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc
    54301 cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc gttattcacc
    54361 aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg aatgccatca
    54421 atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgctcg
    54481 atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc
    54541 cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc aactttacct
    54601 tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc cacaactagc
    54661 gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca ccgaccatgc
    54721 cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag ctcggggaga
    54781 tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc cccatcatgt
    54841 ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc cggcgctcgt
    54901 gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc agccccatac
    54961 tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg cgacggttac
    55021 tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact tttttggtgg
    55081 gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg gcggtatcaa
    55141 tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg cggtgaccga
    55201 tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg ataacaccta
    55261 actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc ccgccaacgg
    55321 aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg tggctgtaaa
    55381 ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg cgcagccctt
    55441 gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc acatcaacct
    55501 ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt cgttttctct
    55561 ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac aatgttttat
    55621 ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa
    55681 aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc aacagattaa
    55741 tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg ttcaatggtc
    55801 aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg ggcattacgt
    55861 ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc tatcgggtga
    55921 ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc aagcatcttg
    55981 ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc gacatcagcc
    56041 aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc aattcccatc
    56101 aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac gggtaaattc
    56161 aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc gtttatggat
    56221 gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa acgggctaaa
    56281 ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa cgtctgaccg
    56341 ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc aatcgccgct
    56401 cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct gatgccatat
    56461 tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc atcccacaac
    56521 ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgtgcacc ggtgcggcta
    56581 tcagtcagaa catgtggtgc gcggctcatg ttttcctggc caccggcgat gacaatatca
    56641 gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc ggaaccgcaa
    56701 acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat agccgattgc
    56761 cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac ttcatccacc
    56821 gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact accaagttcc
    56881 acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg taacgccccg
    56941 acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata gtaaatgatt
    57001 gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa ggaaatatgc
    57061 ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa cccaccaata
    57121 gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa attgccctcc
    57181 attttgttta atttgtagat gatacgttca gataatgtct aatatttggt ctaaagaaga
    57241 aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc tattccttcc
    57301 tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg tcgcctggcc
    57361 tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa aaacatcagc
    57421 aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg gtaatctgat
    57481 taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag tggcaattac
    57541 caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca tccgtatgtt
    57601 ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac gtcatgccac
    57661 tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat ttctttccat
    57721 ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt tcaatcagaa
    57781 tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta gccatacgcc
    57841 cattatttct acgtttgcca tagacagaag tgaaaaatat ggcgaacacg ctatggataa
    57901 atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac tgttctttgt
    57961 ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag aagaaggggt
    58021 caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt caatttccgg
    58081 gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact ttggcgttat
    58141 tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa agaaaagtcg
    58201 tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca ttgtttgttg
    58261 cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca ttgccatgat
    58321 acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc
    58381 catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa tgttctttag
    58441 ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc atttttcata
    58501 atattcattt gttattattc cgccctatca cttctctcgt catatcctag aattatcagt
    58561 tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga tgcataactt
    58621 agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat attctcatac
    58681 catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg aaacataatc
    58741 atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag caaatacaga
    58801 ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat catcaatcac
    58861 caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc tttgtagtcg
    58921 ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc cagaagagtc
    58981 accgcgtttg cagtactgcg caaactcgt
//
LOCUS       DAECOD010000012        58062 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.012, whole genome shotgun sequence.
ACCESSION   DAECOD010000012 DAECOD010000000
VERSION     DAECOD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.012"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(27..281)
                     /locus_tag="KKO15_000723"
     CDS             complement(27..281)
                     /locus_tag="KKO15_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBD6359632.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            436..1008
                     /gene="yeiP"
                     /locus_tag="KKO15_000724"
     CDS             436..1008
                     /gene="yeiP"
                     /locus_tag="KKO15_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBD6359633.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1231..2697
                     /locus_tag="KKO15_000725"
     CDS             1231..2697
                     /locus_tag="KKO15_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD6359634.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2815..3801
                     /gene="yeiR"
                     /locus_tag="KKO15_000726"
     CDS             2815..3801
                     /gene="yeiR"
                     /locus_tag="KKO15_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBD6359635.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3840..4553
                     /gene="lpxT"
                     /locus_tag="KKO15_000727"
     CDS             3840..4553
                     /gene="lpxT"
                     /locus_tag="KKO15_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBD6359636.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4965..5531
                     /gene="mepS"
                     /locus_tag="KKO15_000728"
     CDS             4965..5531
                     /gene="mepS"
                     /locus_tag="KKO15_000728"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBD6359637.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5712..7268
                     /locus_tag="KKO15_000729"
     CDS             5712..7268
                     /locus_tag="KKO15_000729"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBD6359638.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7350..9164
                     /locus_tag="KKO15_000730"
     CDS             7350..9164
                     /locus_tag="KKO15_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD6359639.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGHITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HILQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9165..10259
                     /locus_tag="KKO15_000731"
     CDS             9165..10259
                     /locus_tag="KKO15_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBD6359640.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10259..11284
                     /locus_tag="KKO15_000732"
     CDS             10259..11284
                     /locus_tag="KKO15_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6359641.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11286..12875
                     /gene="yejF"
                     /locus_tag="KKO15_000733"
     CDS             11286..12875
                     /gene="yejF"
                     /locus_tag="KKO15_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBD6359642.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGHCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHIVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12879..13223)
                     /locus_tag="KKO15_000734"
     CDS             complement(12879..13223)
                     /locus_tag="KKO15_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359643.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="KKO15_000735"
     CDS             complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="KKO15_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBD6359644.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="KKO15_000736"
     CDS             complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="KKO15_000736"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBD6359645.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15619..17379
                     /locus_tag="KKO15_000737"
     CDS             15619..17379
                     /locus_tag="KKO15_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD6359646.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCHE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17504..17788
                     /gene="rplY"
                     /locus_tag="KKO15_000738"
     CDS             17504..17788
                     /gene="rplY"
                     /locus_tag="KKO15_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBD6359647.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="KKO15_000739"
     CDS             complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="KKO15_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBD6359648.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19116..19343
                     /locus_tag="KKO15_000740"
     CDS             19116..19343
                     /locus_tag="KKO15_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBD6359649.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19363..21123
                     /gene="pbgA"
                     /locus_tag="KKO15_000741"
     CDS             19363..21123
                     /gene="pbgA"
                     /locus_tag="KKO15_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBD6359650.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21198..21274
                     /locus_tag="KKO15_000742"
     tRNA            21198..21274
                     /locus_tag="KKO15_000742"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21232..21234,aa:Pro,seq:ggg)
     gene            complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="KKO15_000743"
     CDS             complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="KKO15_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBD6359651.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24288..24935
                     /gene="narP"
                     /locus_tag="KKO15_000744"
     CDS             24288..24935
                     /gene="narP"
                     /locus_tag="KKO15_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBD6359652.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="KKO15_000745"
     CDS             complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="KKO15_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBD6359653.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="KKO15_000746"
     CDS             complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="KKO15_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBD6359654.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="KKO15_000747"
     CDS             complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="KKO15_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBD6359655.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="KKO15_000748"
     CDS             complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="KKO15_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBD6359656.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="KKO15_000749"
     CDS             complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="KKO15_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBD6359657.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="KKO15_000750"
     CDS             complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="KKO15_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBD6359658.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="KKO15_000751"
     CDS             complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="KKO15_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBD6359659.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="KKO15_000752"
     CDS             complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="KKO15_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBD6359660.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31445..32047)
                     /gene="napC"
                     /locus_tag="KKO15_000753"
     CDS             complement(31445..32047)
                     /gene="napC"
                     /locus_tag="KKO15_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBD6359661.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32057..32506)
                     /gene="napB"
                     /locus_tag="KKO15_000754"
     CDS             complement(32057..32506)
                     /gene="napB"
                     /locus_tag="KKO15_000754"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBD6359662.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32503..33366)
                     /gene="napH"
                     /locus_tag="KKO15_000755"
     CDS             complement(32503..33366)
                     /gene="napH"
                     /locus_tag="KKO15_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBD6359663.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33353..34048)
                     /gene="napG"
                     /locus_tag="KKO15_000756"
     CDS             complement(33353..34048)
                     /gene="napG"
                     /locus_tag="KKO15_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBD6359664.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34055..36541)
                     /gene="napA"
                     /locus_tag="KKO15_000757"
     CDS             complement(34055..36541)
                     /gene="napA"
                     /locus_tag="KKO15_000757"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBD6359665.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36538..36801)
                     /gene="napD"
                     /locus_tag="KKO15_000758"
     CDS             complement(36538..36801)
                     /gene="napD"
                     /locus_tag="KKO15_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBD6359666.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36791..37285)
                     /gene="napF"
                     /locus_tag="KKO15_000759"
     CDS             complement(36791..37285)
                     /gene="napF"
                     /locus_tag="KKO15_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBD6359667.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37693..38181
                     /gene="eco"
                     /locus_tag="KKO15_000760"
     CDS             37693..38181
                     /gene="eco"
                     /locus_tag="KKO15_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBD6359668.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38331..39362)
                     /locus_tag="KKO15_000761"
     CDS             complement(38331..39362)
                     /locus_tag="KKO15_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBD6359669.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39596..39955)
                     /locus_tag="KKO15_000762"
                     /pseudo
     CDS             complement(39596..39955)
                     /locus_tag="KKO15_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40173..41816)
                     /gene="yojI"
                     /locus_tag="KKO15_000763"
     CDS             complement(40173..41816)
                     /gene="yojI"
                     /locus_tag="KKO15_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBD6359670.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41892..42542)
                     /gene="alkB"
                     /locus_tag="KKO15_000764"
     CDS             complement(41892..42542)
                     /gene="alkB"
                     /locus_tag="KKO15_000764"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBD6359671.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42542..43606)
                     /gene="ada"
                     /locus_tag="KKO15_000765"
     CDS             complement(42542..43606)
                     /gene="ada"
                     /locus_tag="KKO15_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBD6359672.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43680..44735)
                     /gene="apbE"
                     /locus_tag="KKO15_000766"
     CDS             complement(43680..44735)
                     /gene="apbE"
                     /locus_tag="KKO15_000766"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBD6359673.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44847..45968)
                     /gene="ompC"
                     /locus_tag="KKO15_000767"
     CDS             complement(44847..45968)
                     /gene="ompC"
                     /locus_tag="KKO15_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD6359674.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46706..49378
                     /gene="rcsD"
                     /locus_tag="KKO15_000768"
     CDS             46706..49378
                     /gene="rcsD"
                     /locus_tag="KKO15_000768"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBD6359675.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49395..50045
                     /gene="rcsB"
                     /locus_tag="KKO15_000769"
     CDS             49395..50045
                     /gene="rcsB"
                     /locus_tag="KKO15_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBD6359676.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50245..53094)
                     /gene="rcsC"
                     /locus_tag="KKO15_000770"
     CDS             complement(50245..53094)
                     /gene="rcsC"
                     /locus_tag="KKO15_000770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBD6359677.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53369..54145)
                     /locus_tag="KKO15_000771"
     CDS             complement(53369..54145)
                     /locus_tag="KKO15_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBD6359678.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54150..55799)
                     /locus_tag="KKO15_000772"
     CDS             complement(54150..55799)
                     /locus_tag="KKO15_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBD6359679.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55800..56359)
                     /locus_tag="KKO15_000773"
                     /pseudo
     CDS             complement(55800..56359)
                     /locus_tag="KKO15_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56518..>58062)
                     /locus_tag="KKO15_000774"
     CDS             complement(56518..>58062)
                     /locus_tag="KKO15_000774"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD6359680.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14199 a        15664 c        14267 g        13932 t
ORIGIN      
        1 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
       61 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
      121 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
      181 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
      241 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
      301 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
      361 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
      421 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
      481 aacggcaaac tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc
      541 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
      601 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
      661 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
      721 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
      781 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
      841 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
      901 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
      961 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
     1021 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
     1081 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
     1141 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
     1201 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
     1261 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
     1321 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
     1381 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
     1441 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
     1501 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
     1561 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
     1621 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
     1681 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
     1741 cgccgccgtg agcgcagcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
     1801 aatggccatg tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc
     1861 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
     1921 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
     1981 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
     2041 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
     2101 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
     2161 ttcgcctata tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta
     2221 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
     2281 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
     2341 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
     2401 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
     2461 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
     2521 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
     2581 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
     2641 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
     2701 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
     2761 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
     2821 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
     2881 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
     2941 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
     3001 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
     3061 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
     3121 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
     3181 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
     3241 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
     3301 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
     3361 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
     3421 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
     3481 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
     3541 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
     3601 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
     3661 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
     3721 gagctgattt ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt
     3781 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
     3841 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt
     3901 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
     3961 tctttaatca gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca
     4021 atcgcgcctt cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc
     4081 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
     4141 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
     4201 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
     4261 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
     4321 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
     4381 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
     4441 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
     4501 ccttttttga caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag
     4561 taattaacat catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc
     4621 tttcatctat cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
     4681 ctcaattgta ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat
     4741 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
     4801 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
     4861 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
     4921 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
     4981 cgattttgag atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg
     5041 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
     5101 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
     5161 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
     5221 gcagcactaa aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat
     5281 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
     5341 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
     5401 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
     5461 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
     5521 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
     5581 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
     5641 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
     5701 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
     5761 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
     5821 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
     5881 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
     5941 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
     6001 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
     6061 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
     6121 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
     6181 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
     6241 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
     6301 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
     6361 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
     6421 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc
     6481 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
     6541 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag
     6601 tactgctacg ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact
     6661 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
     6721 gcgatgccgc agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg
     6781 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
     6841 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
     6901 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
     6961 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
     7021 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
     7081 acgcggtgct gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa
     7141 cgccggaaca agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
     7201 ttagtcaccc gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc
     7261 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
     7321 aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg
     7381 ctctgttcac cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc
     7441 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
     7501 caaaaggtgg gcatataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg
     7561 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt
     7621 ccgatgacga accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg
     7681 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca
     7741 ttactgcccg cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat
     7801 ttcgtctggt ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg
     7861 agttagctaa acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag
     7921 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
     7981 gtccgtacca cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
     8041 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
     8101 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
     8161 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
     8221 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
     8281 cgtttaatat ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg
     8341 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
     8401 gttattttca aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
     8461 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
     8521 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
     8581 acgaagcggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
     8641 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
     8701 acatcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
     8761 ctaaccgcat gcgcaatcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
     8821 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
     8881 cccccggcgt gcaaagcccg gttatcgact cactgatcaa ccaaattatt gccgcgcagg
     8941 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
     9001 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
     9061 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
     9121 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
     9181 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
     9241 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
     9301 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
     9361 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga
     9421 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
     9481 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
     9541 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
     9601 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
     9661 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
     9721 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
     9781 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
     9841 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
     9901 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
     9961 aaggggtaag tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg
    10021 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    10081 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    10141 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    10201 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    10261 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    10321 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    10381 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    10441 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    10501 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    10561 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    10621 ctggctggga acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg
    10681 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    10741 gggggcgcta caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga
    10801 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    10861 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    10921 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    10981 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    11041 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    11101 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    11161 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    11221 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    11281 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    11341 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    11401 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    11461 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    11521 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    11581 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    11641 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    11701 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    11761 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    11821 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    11881 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    11941 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcactgt gtcgagcaaa
    12001 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    12061 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    12121 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    12181 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    12241 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    12301 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    12361 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    12421 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    12481 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    12541 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    12601 cattaattct taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    12661 cggtacaggc gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct
    12721 atttgtttat cagccacgat ttgcacattg tccgcgcgtt atgtcatcag gttatcgtac
    12781 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc
    12841 aggagtatac gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    12901 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    12961 tggcgattta caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg
    13021 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    13081 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    13141 tcaaccttcg aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg
    13201 attgagagtt gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt
    13261 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    13321 cctcgcctgc gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    13381 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    13441 gactttttac ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt
    13501 gtggttgaca tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc
    13561 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    13621 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    13681 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    13741 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
    13801 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcggcgca ctgatgacca
    13861 tccacgcagc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    13921 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    13981 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc
    14041 cggcgcttaa gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca
    14101 ggacacgttt atggcggaac agcgccgcaa agttaccaat agtggtacga atgtgaaatg
    14161 gctgacgacg ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca
    14221 gaatcgccgc taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc
    14281 agccgccaac tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca
    14341 tcatccgcga gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc
    14401 tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
    14461 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg
    14521 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
    14581 gaatataagt actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa
    14641 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
    14701 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
    14761 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
    14821 cgggggctaa atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca
    14881 cgtggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
    14941 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt
    15001 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt
    15061 ccagtgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
    15121 actgaccatc atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg
    15181 cgtgcagttt ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt
    15241 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
    15301 gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg
    15361 cattacgaac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
    15421 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
    15481 tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccaat
    15541 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
    15601 atctacctac gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    15661 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    15721 gcaggtaaaa gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg
    15781 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    15841 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt
    15901 ggcagcgttc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    15961 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    16021 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    16081 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    16141 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    16201 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    16261 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    16321 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    16381 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    16441 gaagatgctg cactgattac tggcgacaac cccggcgctg agcgcgatgt gttaattgaa
    16501 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
    16561 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac
    16621 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
    16681 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
    16741 agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    16801 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg
    16861 tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    16921 ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccacgaatg tgacacggta
    16981 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
    17041 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    17101 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    17161 cagcgtaccg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    17221 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    17281 tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
    17341 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    17401 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    17461 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    17521 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    17581 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    17641 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctaacca tcgttgttga
    17701 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    17761 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    17821 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    17881 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag
    17941 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    18001 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    18061 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    18121 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta
    18181 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    18241 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    18301 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    18361 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    18421 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    18481 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    18541 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg
    18601 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    18661 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    18721 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    18781 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    18841 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    18901 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    18961 gtattcaacc accgccagtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
    19021 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    19081 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta
    19141 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    19201 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    19261 ttgccccggc ccaacgccag gcaattgcca actcttttgc cagcgcctta cagtcctcta
    19321 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    19381 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    19441 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    19501 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctagtgttc
    19561 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    19621 aggtttttgt ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa
    19681 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    19741 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    19801 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    19861 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    19921 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    19981 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    20041 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat
    20101 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    20161 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    20221 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    20281 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
    20341 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
    20401 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    20461 gtacgcaccc aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac
    20521 gcacaagaag ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac
    20581 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    20641 atcaaacgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt
    20701 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
    20761 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    20821 gctctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc
    20881 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    20941 tgggttactg ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    21001 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    21061 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    21121 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    21181 tattagcagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
    21241 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt
    21301 acggttggtt tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc
    21361 tgtttatttt ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt
    21421 ctacccctgc gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg
    21481 atgaccttat cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc
    21541 catcgtcatt aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca
    21601 gccagggttc cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg
    21661 cttcagcgcg taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt
    21721 agtcctgacc atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc
    21781 cgtcaaccca acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc
    21841 caaacgctgt tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt
    21901 tcaccacccc atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg
    21961 tatagctatc gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac
    22021 caaagaacaa gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga
    22081 gcgtcaggcc cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc
    22141 gggtgttaat tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa
    22201 gacgctcacg cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt
    22261 tcagcacatc agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat
    22321 ggttgccatt atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat
    22381 tgcccaacgt aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg
    22441 ctgccgggct ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg
    22501 tgacgttgag ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt
    22561 ttcccgacag ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg
    22621 aacgagtcac tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta
    22681 agttgaggcg agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat
    22741 tttcgagcga aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc
    22801 cctcagaaac cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca
    22861 tgacctcaag aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga
    22921 cattagcgcg gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga
    22981 tacttccatt ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac
    23041 cagtatcctt tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct
    23101 gcgtgtcctc cataacaatg agcccggaac catcatccag ttcataattt tttgacacac
    23161 catcctttat actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg
    23221 agacaattaa tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa
    23281 cctccagtaa gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc
    23341 gcaaacttcc gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact
    23401 gaccgagacg gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc
    23461 ctgctttctg atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct
    23521 tttcgatagc ggcaaaaacg gtccccccat ctttttcaac gattaagcgc ccgcctttaa
    23581 tcgtggtatc attcgctggg ccataaacct gtagcacacc attattgagg gtagtctggt
    23641 ttgccgcgcc acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg
    23701 taccattcat ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa
    23761 attcttttat atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa
    23821 ccagctggat atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg
    23881 atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac
    23941 aaagagaaac agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt
    24001 aaaagcattg ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact
    24061 gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc
    24121 tgatgcaaaa ttccgtcttt ataataaaaa tgatgccaaa gcgaacgaca aggttgtagt
    24181 tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa
    24241 cgctacgctc aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa
    24301 cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt
    24361 tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgaaggc gcgagcgcga
    24421 tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta
    24481 tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta
    24541 tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg
    24601 gttatctgtt gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga
    24661 aaggcagcaa agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg
    24721 gcgcggaaga agatcccttc agcgtgctga cggagcgcga gctggatgtt ctgcacgagc
    24781 tggcacaggg gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag
    24841 taaaagtaca tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca
    24901 ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg
    24961 ggctgagatt tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    25021 gatcgatcaa tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca
    25081 tccggcataa actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc
    25141 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    25201 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    25261 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg
    25321 atattgctat ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg
    25381 ttgggatcag atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc
    25441 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    25501 atgccaacgc ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga
    25561 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg
    25621 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    25681 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    25741 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    25801 gcttccggca ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg
    25861 ccaatagcca ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    25921 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt tttactttta
    25981 ccttcctgca tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa
    26041 tcggcaatgc tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    26101 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    26161 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    26221 tctcccacag cggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc
    26281 gatagcgaat gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga
    26341 gatccagccc taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct
    26401 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa
    26461 tgccctgcgc tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag
    26521 tcgcccagac gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact
    26581 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    26641 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    26701 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc
    26761 cggcgcagtt ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    26821 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    26881 aagacgcacg gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    26941 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    27001 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac
    27061 accgccgcgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    27121 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg
    27181 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    27241 ataactgaag gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag
    27301 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    27361 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    27421 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag
    27481 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    27541 aatactgccc agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    27601 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    27661 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    27721 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    27781 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    27841 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    27901 cgccagtgag tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac
    27961 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    28021 cacaatgccg agcgtcagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc
    28081 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    28141 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    28201 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt
    28261 agaggtaaag agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac
    28321 acgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    28381 gctcatcagc agcacccaca gcagaagcga accttcatgc gcgccccaag tagccgccac
    28441 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    28501 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    28561 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    28621 cggatacacg gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat
    28681 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    28741 atcgctttct caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc
    28801 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    28861 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg
    28921 gtgaaggtca ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg
    28981 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    29041 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    29101 atagtcagcg ctagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    29161 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    29221 gcgccacacc acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca
    29281 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    29341 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    29401 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    29461 cagcgtggca gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    29521 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    29581 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    29641 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    29701 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    29761 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    29821 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    29881 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    29941 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    30001 gccgacggta agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat
    30061 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    30121 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    30181 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    30241 ccatcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    30301 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    30361 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc
    30421 agcgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    30481 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    30541 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    30601 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc
    30661 ggtgcaatac gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    30721 acaatcagga agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc
    30781 agttcaagac ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc
    30841 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc
    30901 cctgctccgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    30961 cggtaaaagg ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag
    31021 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    31081 gcccggcctg cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt
    31141 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    31201 gggtttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    31261 cagcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt
    31321 tgctaccggt gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata
    31381 aggttcgttc atcccgctca caaagtaact ctctggcttc aagcataccc acgcaataac
    31441 cctgttaaaa acctggctcg acttcacgca tatcgggcag cttgtgcgcg atccctttat
    31501 ggcaatcaat acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg
    31561 atttctgggc ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt
    31621 tattgtcctt catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt
    31681 tctgcggcgt gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct
    31741 tgcgtatcat cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga
    31801 cgccgctacg gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttgcgca
    31861 tttcgtggca gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc
    31921 ccccccagaa gacgatgccg ccaacaaaac cgatcaacag cagcgtcccc agcgccagat
    31981 ggctgggggt acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat
    32041 ttcccataat aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga
    32101 ttggcgcggt atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca
    32161 cttccgcgcc cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc
    32221 cagtggtgcg atagctttcg acaccgtggc actgcaagca gcgattggta ttggtcgtta
    32281 cctgataacc ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca
    32341 tccgatcctg ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg
    32401 actggctaaa atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca
    32461 tcgccgtcca ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga
    32521 actccatcgt gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca
    32581 cgtcatgcaa tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg
    32641 tagcacatgc ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg
    32701 atcgctggcc gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac
    32761 cggacaaatg tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc
    32821 gagaataagc agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagagaaac
    32881 ggggttgatc cattcccaaa tgagcgtgcc agttaaggct gatcccacca gaatgaccac
    32941 caacagcacg tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct
    33001 gcgtaaccag ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa
    33061 tcgctttccc gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt
    33121 ggcaggcaga tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac
    33181 ggtgtcgaat aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccatgg
    33241 accgctcaaa aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg
    33301 gtgactgcgc caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt
    33361 tgccattgtt cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca
    33421 gtgacagcgg taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac
    33481 cgcaaccggt acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac
    33541 gcgtgttgcg ctccagctcc aaggtgatgg cctcatcaat tttcgggcat tcgcgataac
    33601 aaacatcgca gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca
    33661 gccccatccg cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg cttgggcaca
    33721 ctttggcgca cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg
    33781 gcgtgcccgc cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag
    33841 cctgaacaca ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc
    33901 cgggcgggcg caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca
    33961 cacccacggc agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac
    34021 cattttgagg tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc
    34081 acttcttgaa gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa
    34141 ccagctgtgc ggcgtcgaag aacggcatgt acaccaggcc ctgtggcgga cggttacgac
    34201 cgcgcgtttc aacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc
    34261 cacggcgcag atcgcgcgct ttcgcatcca gcgggtgaat aaacaagacc gcttccggga
    34321 aggcgcggtg cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagaa
    34381 cgcgtccggt agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga
    34441 acggcagtgc gaagatcacc gctttgccat ccggtttacc gtagaactta tagccttcgc
    34501 ccgctttcac gtacgggtcg ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca
    34561 ccaccggcca gcgcagaccg cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac
    34621 cgtgaccgcg accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca
    34681 gctcgcggga ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca
    34741 cttcgggggt ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg
    34801 ccagcagctc ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact
    34861 gccagagatc cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac
    34921 ggcgttcggc gttaccgtaa gcgccctctt tctctaccca cattgcggtc ggcaggatca
    34981 agtcggcggc cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg
    35041 gatcgcgcca gcccggcata cgctcttcat tgatgttcgg cccggcctgc atgttgttgg
    35101 tacacatggt ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg
    35161 catgaagacc gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcacaga
    35221 tatcacgatg tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc
    35281 ccacttcacg cgcagtacca cacgcagaag gttgcccggt cagggagaac ggaccgcaac
    35341 ccggctggga aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttagcccaca
    35401 caccacgggt atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat
    35461 cggcatacag ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcagcgg
    35521 ttttttccag cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg
    35581 aggcgtcaga acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt
    35641 agccgatgtc cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta
    35701 tcgcattgtt ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg
    35761 gcgtaaagat gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca
    35821 ccgccacggt gacgttctgg ttagagagac gactgttagt gatgcgtgac cagaggatcg
    35881 ggtgcatctc cgccatgtta gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt
    35941 catagcagcc catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg
    36001 ccatacagtg acgtgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct
    36061 tggacgcggc ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt
    36121 ccggcccttt ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat
    36181 cccaggtgat tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca
    36241 gcggctgcgt caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc
    36301 agttcaggcc acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct
    36361 gctgcgttcc gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc
    36421 atttaatggc ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg
    36481 cagccgccgc agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca
    36541 tggtgtttcc tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc
    36601 ctctacgttg cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac
    36661 caccacaatc aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc
    36721 gttcagttgg gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca
    36781 aacttgccag ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg
    36841 ctgcacatgc tccacatccg ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg
    36901 agagcgtcgg gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt
    36961 caactgactg atacgccagg cacgcgtccc cgatggtgaa ctgcaaatcc caggccctgg
    37021 tgtggcgcgg agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc
    37081 actcgttatt tttgaaatta acgctcggat agccacccgt gccgcgttgc agaatgttgt
    37141 tttcacaagc attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat
    37201 caccggacca gggcggacgg ataccgttac tggctttgcg ccagcaacca gtgagtatgc
    37261 cccgacggga tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg
    37321 agagcgacca aatagaccgc ataattaata agccattttt atagccgcta agatattaaa
    37381 ggatgtgtca aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg
    37441 gcgggaagcg tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta
    37501 tatagcgatt aattcaccag agatatttct gctggtttgc tccctcatca gaatttaaca
    37561 ctaaaagagc aggtaaaatt gtctgaatgt tctttaagtt attcataaag caaattaata
    37621 aatctgatga atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag
    37681 gaaagcaaaa aaatgaagac cattctacct gaagtattgt ttgccgcttt cgctaccact
    37741 tccgcctggg cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct
    37801 gaaacaggca tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg
    37861 aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc
    37921 gggaagctgg aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa
    37981 gttagctcgc cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt
    38041 gtcaccgcgt atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg
    38101 gtgtatacgc cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt
    38161 gacaacgcgg aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc
    38221 ggttacgccg catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt
    38281 cttatcagta tacgccacat ccggcatacc atgccggatg tggcacatca ttacaacgca
    38341 atatccgcca cttctttttg aacgggttgc ggtttcaact gcggtttcgg cgtactatct
    38401 gctgcttgcg gtttgtcgta tttcagcccc agcacttcgc tggtgtactg caactcgcgt
    38461 tctgttgccg ctacatcacc gttcagcttg cgtccataag acggaacgat cgctttcaac
    38521 gtagcctgcc attgcgggct ggaaacgcga tcgccaaata ctttttccag tagattcaac
    38581 ataatcggcg cggcggttga cgcccctggc gatgccccca gcagcgccgc aatggtccct
    38641 tgctggtcac tgaccacttc ggtacccaga cgcagtacgc cacctttctc ggcatcacgc
    38701 ttgataatct gcacacgctg ccccgcttgc cacaaacgcc agtcctcttt tttcgcttgc
    38761 ggatagtact ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac ctgactcacc
    38821 agatatttca ccagatcgaa attatccagc ccgacgtgca tcatcggcat cacgttagag
    38881 gtggtggtgg aactcatcag atcccacaat gaaccgtttt tgaggaattt ggttgagaag
    38941 gtggcaaatg gcccaaacag cactacgcgt ttaccgtcca gaacacgggt atcgatatgc
    39001 ggaaccgaca tcggtggtgc gccaacggat gctttaccgt aaactttcgc cagatggcga
    39061 ttaaccacgt ctggattttc cgaaacaagg aactgtccgc ccaccgggaa accggcgtag
    39121 tctttcgctt ccggaatccc cgattcctgt aacagcttca gcgccgcacc gcccgcgccg
    39181 ataaagacaa atttggcgcg gatgttctgt gcagtgccgt ttttcagatc ggcaacggta
    39241 acggtccagg tgttatcgtc attacgcttt agggcgcgga cttcgctgct gagttgcagc
    39301 gagaagttag atttcttctg taaggaagca attaactggc gggtgatctc accatagttc
    39361 acatcagtgc caatttctgt tgcggatcgc gcccttccat cactaacggt gcccactctt
    39421 tgatctgcgc gtgatcttca gagtaacgca taccgcgaaa cagcgagctt tgttgcaacg
    39481 cggcgtaacg ggcgcgcagg aaattaacgt tatcctcgcc ccagacaaag ctcatatgcg
    39541 gaacggtatt gataaatgaa cgcggagtac gcagcacgcc gcgctcgacc tggtgcgccc
    39601 agaactggcg ggaaatctga aatgcttcgt taatggcgac tgctttttca atactgatgc
    39661 tgccatcggc gttttgcggg gtgtagttca gttccatcag tgcagaatgc ccggttccgg
    39721 cgttgttcta gccgttcgaa ctctcctgcg cgcccccctc caggcgctcc accatggtca
    39781 tcgaccattc aggctccagc tcgcgtcaat aggtccccaa cgtggcgctc ataatgccgc
    39841 cgccaatcaa cagtacatca gtttcctgct cctcggacgc tttcgctttt gccgccatcg
    39901 aaacggcatt aagccccacg gccatcgaga agagcatggc agtcactttt ttcatcttgt
    39961 taatgcctta cttttagtcg ctttattgca ggtgagattt gcgcggcatc aacggtaaca
    40021 cttaagtaac aacatttaaa taatgtttat aaattatatt ctaattttag aaacattata
    40081 atttcgttga ttaattatag ggttattaga aatgaaggga tttttagcat attgacctga
    40141 cggcagcagt ccgccaggtc agcgtcgtaa tattatgccg tccgggcaac ggcatcacgc
    40201 gaagcggcat cgcgctcttc gcccgtcagc tcgctaagtt gcccattgcg catttccagc
    40261 aggcggtcgg cgtggataaa gtaatgatca tcatgactga tagcgaaaat agttttaccc
    40321 atctcctgca tcagcggcag caacacctga taaaactcac gacggaagtg tggatcctga
    40381 tccaccgccc attcatccag caggataata tcgcgttctt ctgccagcgc cagcaacagc
    40441 gccacgcgtt ttttctgccc ttttgataac ttcaggttaa caatacgccc gttgcttaac
    40501 tcaagcttat gagccatttt cagctgcgcc agccacttct caaccagttg cgggttagcg
    40561 ggttgcccct ccggccccag cagttgatca aacagccaga catcggtaaa cactgccgaa
    40621 aacagtttgc gataatcttc cggttgttcg ccgctgacag gtttgccatc cagcaagatt
    40681 tcgccgcttt gtggctgata caagcccgtc aacaacatcg ccagcgtcga ttttccgcta
    40741 ccgttaccgc caatcagaaa cagcagctcg ccacgtttga tggtgagatt aatcggacca
    40801 acggaaaacg cgttatcctg ataagcaaac gtcacgttac gcagctccag cgtttgccag
    40861 ttgggaaacg cctgcgggcg cggaaactca gctttgaaag gcgcgagcgc gaatttgttc
    40921 agcttgttaa acgccacctg cgccgtcagc agcgtcggca atgcgccaac cgccgaaagc
    40981 agcggcgtac gcaggaataa cagcgtcaac gaataggtcg cagcaacgtt ggtatcagcc
    41041 caaccgagac tgttcgccat ccagaacacc aggccgatag cgcccagcat catgatgttt
    41101 gaccagttca cggcactaag atggaaggtg tctgcgcgaa taatatgatg gcgatactct
    41161 tgcgcatcgg ggatgtagag gttgttaaac acatactcgg cgcgttcccg gttcagagtc
    41221 agctctttgc gcccttcaag tacagtttga aaatccgtgt acagcttgtc ttcggtttca
    41281 cgtagggtcg ccatatgttt gtacacccgc gccaccagca caaaaccgcc ccagatggtg
    41341 atcgccatcc agatagccgt taccagcaac attttgcccg acagcatcca cagatacgcc
    41401 gatgaaccga tagtgagaat gatcccctgc cccagttccg gcagacgcac aaaagcaatg
    41461 gtgatattgc gcacatcgct ggttaatccc gccagcaacg aggcgctacc gagttgttca
    41521 atgcgctcga cgtgggtatc cagaatccgc ttgataaatt cactacgcag tcggtaaacg
    41581 aagtgatgcc ccaaagtggt gagcgccagt tgcgatccga gagtgactgc catcaacagc
    41641 agcaataatc ccagaaactc cggtaacacc agcagactgg tatccgccgt ttcgataagg
    41701 cgctgattga taaaagcaat taagccaatg cctaatgccg cactggcgag gcttagcgcc
    41761 atcacactga taaatggcca gcgatactgc cgccagacaa gtacaagaag ttccatgcag
    41821 aaaacccgga caatgaatta cagcccgcag tttaaacatc ttcgcgcgca cagcaataat
    41881 aattcttatt tttattcttt tttacctgcc tgacggaatg tcaggttgta gcggcagtcg
    41941 gtggtgagtg gatgaaaccc cgctttcaac ggttgaatac cgtgataaaa cagccgcgat
    42001 tcaccgcccc ataccaccac atcgccatgt tccaacaaca aacgtttgag cggatcatta
    42061 cgtttcaggc caccaaattg aaaaatcgcg ggtaagccca gagaaacaga aacaattggc
    42121 gcgcgcagat ccggttcgtc tttatcctga tgcagcgaca gtttcgcgcc aggagcgtag
    42181 cggttgataa gacaagcatc tggctggaaa tctggatagc ccgccgccgt agccgcacgt
    42241 tgacataaat catgaaagct ttgtggcatg gcgggccacg gtttatttgt ttgcggatca
    42301 atgggcgaat agagataacc ttgccgatgg gtcgtccagc ccagatgccc acagttggtc
    42361 atcgccaccg acatggtata tccgcctgga gcgaccatct ggcgaaacgg cgactggctg
    42421 gcaacgtcat taatatcgcg gattagttgc tccgcagcat taaaagcaaa acgccgtaaa
    42481 attaccgcac cagccgccag tggctcttgc cacggttcag catcggcaaa cagatccaac
    42541 attacctctc ctcattttca gcttcgcggc gcagcagttg cgctttacgc gacacgcccc
    42601 agcggtaacc ggaaagtgtg ccatcaccac ggaccactcg atgacagggt attacgatag
    42661 ccagcttgtt ggcggcacag gcgctggcaa cggcccgtac cgctttcggt ttgccgatgg
    42721 cgttagccag ttgctgataa ctgacggttt caccacaagg tatcgtgcgc agtgcctgcc
    42781 agacttgttg ctgaaaagca gtgccgcgaa tatccagcgg taacgtcagc ggcgtatcgc
    42841 gttgattgag gctggcgatc acttcacgca catgttgctg aaacgtcaga tcggcaggcg
    42901 cgttgtcggc agcgggaaac atctgctgca actcgctgat taatgtcgcg tcatcatcgc
    42961 ccagcaatat cgcgcaaatc ccccgctcgc tttctgccac caggcaacga cccagctcac
    43021 aatcagccag cgcgtaacgc accgccagat tttcgccacc gtgacggaat tgtttagccg
    43081 tcatgcccag cgtttcatcg gctttgcggt agtaactgct gctgtcgggg aatccggcgt
    43141 taagaataga cgtcgtcacg ctctcccctt tcgccagcga ttcgcgcaaa cggcgagcgc
    43201 gccaggcctg ttgccaggct ttaggcgtca ttccggtagt agctttaaac aaccgatgta
    43261 gatgaaatgg actcatcgcc acctggtcgg ctaaggcttc cagcgttaca ggcgtttcct
    43321 gttccagcag tcgacacgcg tgggtgattt tatccaaccg atgttgccgg ggattggctt
    43381 tgtctggctg acaacgtttg caggggcgaa atccggcggc gagtgcctcg ctggcatttg
    43441 cgtagaagga gacattttcc cgcaaggcat gtctggcgcg gcaagaagga cggcaaaaga
    43501 tacccgtggt gcgcacggcg aaaacgaatt cgccgtcggc attcgggtcg cgggctaaga
    43561 cagattgcca gcgttgatcg tcagttaagc atgtggcgtt tttcataatc agctccctgg
    43621 ttaaggatag cctttagtct gcccggtcac catcacgcaa aaaccaacaa tcttgcgctt
    43681 taattttttt cgctgacaag gaagctttta aactgtggtg acatccaggt tttaaagcta
    43741 tcgccttctt tggtgatcat ataaaccgcc agcccttccc ggcgaacaac ctctttggct
    43801 ttctccggcc cgagtaccat caagccagta tcccaggcat cggcttccag cgccgtcgga
    43861 gcaatcaccg tcacggatac cagattgtgt tcgatgggac gcccggtttg cggatcgata
    43921 acatgggaaa gacgtttgcc gtccagttcg taatagttac ggtagctgcc agaggtgcta
    43981 atcccatgac cgttgatatc caccacagcc tgaaccgcgt tttctttatc ggttggtttt
    44041 tgaatcgcta cccgccacgg ctggccttca ccgttcatac cacggctgtt cagcgcgccg
    44101 cccaccgaca ccagatagcg ggaaatccct tcctgctcca tcaagcgtgc caggtgatcc
    44161 gccgcataac cttcaccgac ggtggagaga tcgacatata aatccggcag gtctttttgc
    44221 agatattgct gatgcgactg attgatgacc gtcaggtgct gtaagccggt tttggctttc
    44281 atcgcatcga tctgttcctg gctcggaatt tgaaccggct gttgttccgg gccaaagccc
    44341 cacagattca ccagcggccc gacggtaata tccatcgcac catcggtcct tgccccaatg
    44401 cgcagcgagg tggtgacgat atcggccatc gcttcactta ccggccacgg cgacaaactt
    44461 tgcgagtcgt taaagcgcat cagcgcggaa tcttttttat aggtcgaaag cagctgatcg
    44521 tcggcgtcca gctgggtctg aatcttttct ttaagttcgg cactgcgttt ggcatcaatg
    44581 cccgggatgc tggcacgcca gaaggtaccc atggttttgc cttcaagaac ggtaacttca
    44641 gtagcgtggg ttttggcggg ttgtggtttt tgatcgcaac caacaaagaa gagcgcggca
    44701 accagcagtg ccacgcgggt aaagcttatt tccatacgtg attatcctca tgcgaacggt
    44761 cgcaagagta caccaaaaaa ctgtgtttgt acgctgaaaa caatgaaaaa agggcccgca
    44821 ggccctttgt tcgatatcca tcgagattag aactggtaaa ccagacccag agctacgatg
    44881 ttatcagtgt tgatgccagc gtcacgagtg aactggttgt cgtccagcag gttgattttg
    44941 tagtcaacgt aggtggacat gtttttgttg aagtagtagg tcgcaccaac atcaacatat
    45001 ttcaggatat cttcgtcgtc gtagttacga ccattgatga cacccaggtt tttaccttta
    45061 gactgcaggt aagccaggga cggacgcaga ccgaagtcga actggtactg agcaacagct
    45121 tcgaagttct gtgctttgtt cgcccaaccc aagttgccta cgcgagttgc gttgtaggtc
    45181 tgggtgtact gagcagctag gtagatgttg ttcgcgtcgt atttcagacc accggtgtag
    45241 gtttcagcgc ggtcgccatt accgatgtaa cgctcatcat aacggttcag accgaagttc
    45301 tggtcatcag tacgtttgga gctggaaact gcagcaccga taccgaagcc ttcgtaatca
    45361 taagtaatag aaccgccaac gccgtcaccg ttctgacgca gagcttcacg accgttgttg
    45421 gtcatgcctt caccttccgg gctaccgttt ttgccctgat actgaacagc gaagttcaga
    45481 ccgtcaacca gaccaaagaa gtcggtgcta cggtaggttg cgaagccgtt accacgctgc
    45541 tgcatgaagt tgtcagaacc gtaagtgtca ccaccgaatt ctggcagtac gtcggtccag
    45601 gaagttacgt cgtaaactac gccgtagtta cgaccgtagt cgaaagaacc tacatcctgg
    45661 aatttcagac ctgcgaatgc cacacgggtc caggagttgt tttcgttttc agcgctgttg
    45721 ccctggatct gatattccca ctggccgtaa ccggtcagct ggtcagtaac ctgagtttca
    45781 cctttgaagc caagacgcat gtaggtctgg tcgccatcta cagacttgtc gtcagagaaa
    45841 tagtgcaggc cgtctacttt accgtacaga tctaatttgt tgccgtcttt gttgtaaact
    45901 tcagcagcgt ttgctgcgcc tgctaccagc agagctggga ccaggaggga cagtacttta
    45961 actttcatgt tattaaccct ctgttatatg cctttatttg cttttttatg ccactgcata
    46021 ctgattaacc ctcattaatc agtcggcaag tccattctcc ccaaaaatgc agaataatcc
    46081 aacacgaata tgatactaaa acttttaaga tgtttcattt atcgctatag atgtttcaaa
    46141 atgtaaatac aagggaactt tttaagatta ttgcggaatg gcgaaataag cacctaacat
    46201 caagcaataa taattcaagg ttaaaatcaa taacttattc ttaagtattt gacagcactg
    46261 aatgtcaaaa caaaaccttc actcgcaact agaataactc ccgctatcat cattaacttt
    46321 atttattacc gtcattcatt tctgaatgtc tgtttacccc tatttcaacc ggatgcctcg
    46381 cattcggttt ttttaccctt ctttacacac ttttcattat tctgtgctac cacagaaaaa
    46441 ctataacgct tgttaactat ttcacaaata attaacatca gcataatttc cagcaatctt
    46501 tgtttatttg caattaattt cgttgggctt tttgtaggtt atttgtacag caaaatggcg
    46561 cttgtacatc tatttccccc catacaggat gataaatatc acgggagaat agagaatcat
    46621 caatcaggta agagtctgga atttcacact gtacccttta tactgcccta tcacttcgcg
    46681 aagttttaac aggtcataaa cacgaatgcg tcagaaagag acaacggcca cgacccgctt
    46741 ttcactccta ccggggagca ttacccgctt ctttttactg ttgatcattg tgttactggt
    46801 gacgatgggg gtaatggtac aaagtgccgt taacgcttgg ctgaaagata aaagttacca
    46861 gattgtcgac attacccacg caatccaaaa gcgcgtcgat acctggcgtt acgtgaccag
    46921 gcagatctac gacaacattg ccgcgacgac ctccccctcc tccggcgaag gtttacaaga
    46981 gacgcgcctg aaacaggatg tctactatct ggagaaacca cgccgcaaaa cggaagcgtt
    47041 aatctttggc tctcacgaca actcaacgct tgagatgact cagcggatgt ccacttatct
    47101 ggatacattg tggggtgcag aaaatgtacc gtggtcgatg tattacctga atggtcagga
    47161 taacagtctg gtgctgatct caaccctacc ccttaaagat ctcacctccg gatttaaaga
    47221 atcgaccgtc agtgacattg ttgattcacg tcgtgcagaa atgttacaac aggccaacgc
    47281 cctcgatgaa cgcgaaagtt tttctaacat gcgccgccta gcctggcaga acggtcatta
    47341 ctttaccttg cgtaccacgt tcaaccagcc gggacatctg gcaacggtcg tggcttttga
    47401 tctgccgatt aatgatttga tcccaccggg tatgccgctg gacagtttcc gccttgagcc
    47461 agacgcgacg gcaacgggaa acaatgataa tgagaaagaa gggacggata gcgtcagtat
    47521 ccactttaac agtacgaaga ttgaaatctc ctcggcactc aactctaccg atatgcgcct
    47581 ggtctggcag gttccttatg gcaccttatt gctggatacg ttgcaaaaca ttctgctgcc
    47641 actgctgctg aacatcggtt tgctggcgct ggcgttattt ggctatacca cattccgcca
    47701 tttctccagc cgcagtacag aaagcgtccc cagcacggcg gtcaataacg aattgcgcat
    47761 tttacgggca atcaatgaag agatagtctc actgttgccg ctcggcctgc tggttcacga
    47821 tcaggaatcg aaccgcactg tcataagtaa caaaattgcc gatcatttgc tgccgcatct
    47881 gaatctgcaa aacatcacca ccatggcgga acagcatcag gggattattc aggcgacgat
    47941 caataacgag ctgtatgaga tccgcatgtt ccgcagccag gtcgcgccgc gcacacaaat
    48001 tttcattatt cgcgatcagg atcgcgaagt gctggtaaac aagaaactca agcaggcgca
    48061 gcgtctgtat gagaaaaacc agcaggggcg gatgaccttt atgaaaaaca ttggcgatgc
    48121 gctgaaagaa cccgcacagt ccctggcgga gagtgcggct aaactcaacg ccccggaaag
    48181 caaacaactg gcgaatcagg cagatgtgct ggtgcggctg gtcgaagaaa tacagttagc
    48241 gaacatgctt gcggatgata gctggaaaag tgagacggtg ctgttctccg tgcaggattt
    48301 aattgatgaa gttgtgcctt cagtgttgcc tgccatcaag cgtaaaggtc tgcaactgct
    48361 gattaacaat catctgaaag cacacgatat gcgccgcggc gatcgcgatg ccttacgacg
    48421 tattttgctg ctactgatgc aatatgccgt gacctcaacg caattgggaa aaatcaccct
    48481 tgaggttgat caggatgagt cctctgaaga ccgcctgaca ttccgcattc tggacacggg
    48541 agaaggcgta agtattcatg aaatggataa tttgcacttc ccgtttatca accagaccca
    48601 aaacgatcgt tatggaaagg cggacccgct ggcattctgg ctgagcgatc aactggcacg
    48661 taaactgggc ggtcatttaa acatcaaaac gcgggatggg cttggtacac gctactctgt
    48721 gcatatcaaa atgctcgccg ctgacccgga agttgaagag gaagaagagc gtttactgga
    48781 tgatgtctgc gtaatggtgg atgttacttc ggcagaaatt cggaatattg tcactcgcct
    48841 gttagaaaat tggggtgcaa cctgtatcac acccgatgaa agattaatta gtcaagatta
    48901 tgatatcttt ttaacggata atccgtctaa tcttactgcc tctggcttgc ttttaagcga
    48961 tgatgagtct ggcgtacgag aaattgggcc tggtcaattg tgcgtcaact tcaatatgag
    49021 caacgctatg caggaagcgg tcttacaatt aattgaagtg caactggcac aggaagaggt
    49081 gacagaatcg cctctgggcg gagatgaaaa tgcgcaactc catgccagcg gctattatgc
    49141 gctctttgta gacacagtac cggatgatgt taagaggctg tatactgaag cagcaaccag
    49201 tgactttgct gcgttagccc aaacggctca tcgtcttaaa ggcgtatttg ccatgctaaa
    49261 tctggtaccc ggcaagcagt tatgtgaaac gctggaacat ctgattcgtg agaaggatgt
    49321 tccaggaata gaaaaataca tcagcgacat tgacagttat gtcaagagct tgctgtagca
    49381 aggtagccta ttacatgaac aatatgaacg taattattgc cgatgaccat ccgatagtct
    49441 tgttcggtat tcgcaaatca cttgagcaaa ttgagtgggt gaatgttgtc ggcgaatttg
    49501 aagactctac agcactgatc aacaacctgc cgaaactgga tgcgcatgtg ttgattaccg
    49561 atctctccat gcctggcgat aagtacggcg atggcattac cttaatcaag tacatcaagc
    49621 gccatttccc aagcctgtcg atcattgttc tgactatgaa caacaacccg gcgattctta
    49681 gtgcggtatt ggatctggat atcgaaggga tcgtgctgaa acaaggtgca ccgaccgatc
    49741 tgccgaaagc tctcgccgcg ctgcagaaag ggaagaaatt taccccggaa agcgtttctc
    49801 gcctgttgga aaaaatcagt gctggtggtt acggtgacaa gcgtctctcg ccaaaagaga
    49861 gtgaagttct gcgcctgttt gcggaaggct tcctagtgac cgagatcgct aaaaagctga
    49921 accgcagtat taaaaccatc agtagccaga agaaatctgc gatgatgaag ctgggcgtcg
    49981 agaacgatat cgccctgctg aattatctct cttctgtgac cttaagtccg gcagataaag
    50041 actaatcatc tgtaggccag ataagacgcg ttagtgtctt atctggcatt tgcaccgatt
    50101 gccggatgcg gcgtaaacgc cttatccggc ctacgattcc cattatttca acagattaca
    50161 ttaaagtagg ccagataaga cgcgtcagtg tcttatctgg catttgcact gaatgccgga
    50221 tgcggcgtaa acgccttatc cgtcctacga atcccgcgat ttcctgaccc tctcggcata
    50281 taccgtcagc gtctgtttta tcacatccag cgttaccggc ttcgagaggc agctgtccat
    50341 accggactcc agacaccgct gcttctcttc agccaacgca ttagcagtta ctccgattac
    50401 cggcaacgtc agtcccaact gacgaatgcg ttgcgtcaag cggtaaccat ccatatttgg
    50461 catgttgacg tcgctaagca cgatatcaat atgattctta ctaagtacat taagcgcatc
    50521 gacgccatca ttcgcggttt tacattgata gcccaacgat cccaactgat ctgccagcag
    50581 acgtcggtta atcggatgat catccacgac cagaatcatc atatcgtcgt tatcgctgac
    50641 cgctttgtcc gtcgacggca gagcgttagc aggatcgtcg ctctccatct cgatcaaata
    50701 aatacgcgcc aacaatgccg gtagctcatg cggagcagcc acactgtgta cccactcccc
    50761 tggcgctttc tccagcggaa taccaatatg gcgacgacag aaggtcacta ccgctctgcc
    50821 ctgccatttt ttactcacta cctcgtcagt gattaacaca tcttcgggag tcggttcctg
    50881 cccttcgtat gttgtaacga cgatgccgct gcgctgcaaa ctggtttcca ggaactgaca
    50941 gagcgacgca ttgcggaccg ccagccagca gcgtttacca ctcaaccctt ccacgccttt
    51001 tttctgcggg tactgagcgc cgtacaacgg aatacgcacg gtaaactggc tgcccattcc
    51061 cggttctgaa tctaccgaga tatcgccgtc catcatgctg atcagttttt cacaaatcgc
    51121 cagacccaga ccggtcccct ggaagtttcg ctgtacgccc gttccaacct ggaagaaggg
    51181 atcaaacaag cgcaccactt ctttcgccgg aatccccacg ccggtatcgc gaacacggat
    51241 agagagataa tcgccatcag cgcgaacatg caaaactata cagccggtat cggtgaattt
    51301 tatggcgtta ctcaacaggt tggagatgac ctgctgtaaa cgcatcgggt cgccatttaa
    51361 ggccactggc acatccggtt caataaagca gtacaagcct aactgcttgc gtaccaccag
    51421 cggtaaatag ttggcggtga tgtggttcat cacttcacgc ggtgaaaact cacgcggttc
    51481 gatcttcaac tgttccgatt caatcttcga gaaatcgaga atatcgctga taattttcaa
    51541 caacaggctg gaagagttgt tcattgccgt caccagacga tcgacgcctt tcggtaactc
    51601 tttggtttgc aacagatcca ggttaccgat aatgccatac agcggcgttc gcagctcatg
    51661 actgacggtg gcaaggaaca tcgattttga ctggctcgcc tgttccgctg cttgtgccat
    51721 ctcctgcaac gactcttcca tcttcacgcg cgaagaaaca tccaccagca cacaaatggc
    51781 cacgttttca ttacgatagc gcgaatggac gaagctgatt tgcagattgg tattgttgct
    51841 ggtcaggaca tcaacaaaat tgacctgctg cccacagata atttgcgtca gtcgttggcg
    51901 gtcctcatgc gtaagcatat tgagataggt atgcgccagt tcgttactta aaatattgac
    51961 gccatcagcg gtacgcaaaa tgcagatacc cactggcgcg gaggcgacaa tcttgcgatt
    52021 gaactgctca tgttcttcca gtcgcagggc gtcgctttcc gccggaatga aaatacgtcg
    52081 ctcgtacatc cgcgcgagag taaacaatgc agctccggca agcacattca gcaaaattgc
    52141 gttaaggatc aacatgcgaa tgcgttccag caccttatca accggcaccg aatacacgat
    52201 gcttagcgat gagggtggca gatttttctt cagcaccagc tcccggaacc cttccgtata
    52261 gccaaaccag gagcgttcct gcatccagcg aggatcgccc ttaattttac tttctggtcc
    52321 ggtaagcgaa atcagggtat gaccattttc atcaagaatg gtaaccccca tcggcaacgt
    52381 acccggtaag aaaaagttct ccatccggat ggtctgctcg acacccaaaa gcgcctgcaa
    52441 ccggttcgcc agataaactg gcgtcaacgc gtaaaaatac ccgacgcaag ggcgcggacc
    52501 ttcgctgatc cagtagaggt tactgccgct atcatcttgt ggtgcatttc gatatttatt
    52561 gatgcgttca tgcaacgctt tcaacgcggt atcgcgttcc actggcatat cacgcagacc
    52621 gaaattggcc atgcagaggt tatcactgcc gattaaaaat acccggttga gatcgtaagc
    52681 cgcagaaaaa ttatcgcgcc agtagcgcat aaaccacgcc aatgactcca gagaacctcg
    52741 ccaggtgtta ctcattgcgg aacaatcgga gtcggcaaac agcggttcaa acgcaggcac
    52801 atccgcctgc gtttctcgtc cacgcgggga aagcacaccg ttttctgccg ataagcgatt
    52861 ttcggcgatg tacttaagct ctttcatcac atcagaggtg cgttgaataa agcgctgagc
    52921 ctgatcggaa ctcagattaa attcctgacg aatttccgat tctcgctgat gtaacgcatt
    52981 aacgatgtaa aaaacggatg aaaaagcaat caacagccag agcactaacg ccaatgctct
    53041 gaacatgtag cgcgaggctt tcagggttgt acgaaaagaa gcaaggtatt tcaaagggga
    53101 gaagctccgc ctcaggtgac cgatggagtg tggttaaggt agcggtaaaa gcgtgttacc
    53161 gcaatgttct gtcaccattg caataatgcg tatcgcttag aaaccattgc cttaaccagg
    53221 aaagccggag tagatggaca gttctcagta cgaaaaataa gaaatgacca atctgccgac
    53281 gcttgttaat ggttatatcc tggcgtcggc agatcgccca gcagtcttat ctgaaaaaag
    53341 agagagccag gttgtgtgaa gggatggatc accagtcaaa acttttcacc agcgtcagct
    53401 cgccagcatt acgcatcggt acaataaatg tttcctgttt ctcattgacc gatccttcat
    53461 cggtgatcag cgtcagttgg gcggtcgtca attccgtttc gctgcgccca ccatagtagt
    53521 tgatatacac ctgatagcgc ccgtgaactg gtgctggcat ggcgaaaatc tcgggtccgt
    53581 accccgtcgt gacatccata tccagtgcac cactgttttt cagcacggtg ttaccgtacc
    53641 aggcgtgttc gccatcgggc gtgacaacat ggagatcgag atcggtattg tccgtatccc
    53701 acgagagaac cagccgtaaa cgtgcacgaa tagttcctgt acccggcgtt gagtaaaact
    53761 gcattttttg ccggctttgc ccatccgggc tgatgacctg cacgctgttg ctgccttcag
    53821 tgaaaatata agggcgtgca aatgaaccat cggattcaat tctctgcggc atactggcac
    53881 cgttgaccac cagccggccc tgctgaactt tacccgccgc agcataattc tttattttgc
    53941 cgcggatctg cgctgagata ttttgatcat ctgccatgtt gacggacgac gccgggtaat
    54001 tgatcgattg tgaaaaactg gcatcttcgc cctctgccgg atgccagcca gagagcggtg
    54061 cgtcaatctc gacttcctgc acaccttcac tgtgagcgac aggcgaaagt gccaccagta
    54121 acaacggaag aaaaatcttt cgcatagcat tagtccatta ataattgtcg ggtcagcgtt
    54181 tcgatatagt tctcatcaag cccggtggga taaccatcaa aggccaagtg cagatactca
    54241 tgggttaaat ccagccgatc ctgaagcgta aagaagttac gaataaacag ccgtttctgc
    54301 tgccgatcgg tataggggaa acctgagact aaacgacaga ccgcaaacac gtctggttcg
    54361 ttgtaccccg tctcgccttg taatatacgc cgccactgcg gcattttttt cgccagccag
    54421 gctttcgctt tgggtaataa ctggcaggtt gagcgtggag ctccccagcg gctaaggctg
    54481 ttgtcgggat aagcaaacgc gagtatctgg tcgtaacgct ccccctgccc cgcctgggcc
    54541 gtggcttgcc gccaggaaag tgtcccttcg gtagcgcgac tgccatgata atgaaccgga
    54601 tcgcctgcat agatgagctc ctgcgtccag gcggtcatcg ttcgcgcccc cgtggtcgct
    54661 ggcgaagcag aaacacgctg cgtggcgctg ctgtcgggga tggtcaggca atcaccttcc
    54721 cgattggcgt tttgctgtaa aaaagtacgt atcgccacag tcatggcttt cgctgcctca
    54781 ggtggcgtac ttttcgcctc gcgatccaga actctggtaa cgtactcttc acgatcgaga
    54841 tgcgactgca atttcagttt gcccttttcg ctcagtaagg tcgtctcacc gtggctgaca
    54901 aacgtaatgt ggttaccatt agtaaacgtc acacgatagc gcccattaag tacgctgggt
    54961 tttactgccg tcgtactctt ttccgctgtg attttcttca gcggatagcg ggcaaacagc
    55021 tccacctcta cacattgccc actggcaacc tgcgtcggca ctggcaaaac ccgattaagc
    55081 accgtcgcat aacgggttag taccgttttg ctggtgccac tgccagtaac ccacagcggt
    55141 gtaccatcgg ttaaccagcc agcaaaacca ccctgacgga tttcctgttt atcatcggca
    55201 aaccagctcc aggttttcac ccgtacccga ctaccgagca tggatgcgac accgattttc
    55261 gcctcgtcca gcaccacatc cagtaagact tcctgagctt tgttttgcgc aggtaacgtc
    55321 gctaatgaat caagcagcga tttaaccgtt accgaggttt ctggcttcag cgttgtaaga
    55381 gacgctaacc acgcaggtgc ctgacgctgt tgccagtatt gcccccacac atctgcgcca
    55441 atatgcaacc gctgcggcgc aaaatagagc ccacaggaac gtaccagcgc ggtatcgcgc
    55501 gttatgctct ccccagcctg acaacaataa acctcttccg gggagtttcc gcgacactgg
    55561 tagggctgct ccggttgatg ggtatcctcc agccaggcgt aaacatacag tttccacaaa
    55621 ctccctaacg gcgtggtgag cgagtcaggt aatgctgaga ctttggtcac gcctgatgac
    55681 gacagctgat acagttgatc gtgctgcgca ccgcgcagca ccagttgtaa cggcgtctcc
    55741 tccgcaagcg ttggcagagt aaccaacgcc aatagccaga cgattctgcg ccagttcact
    55801 tatttcacct gcatcccggt ccattcactc cctgccgcga cactttgctg cgcaggtgca
    55861 taggaacgca cataacgagc aggaggcaga acgaattgcc ctttttgcga gaagcgcagc
    55921 aaatggcgga aagtgaccgt tcccgtcagt tctttcaccg gcaccatata ggccagttcg
    55981 cccatttcat tacgcgcttt ttccagcaat tgcccctgct gtttcgcggc gttgggttta
    56041 ttgactgaaa tgccccatgt tgtgcgctca acgtcggctc ccggcgggag cggtacttcc
    56101 acctgaccat agcgcagaac tgcatcctgc tcgctggtaa gcgtaatttc atcgagatac
    56161 agcgcatcgc tgtcaatctc attgctggtc accggctgca gagtaaagct catctcttct
    56221 tcaccgggga taagccgata caactggcgt tcaacggtca ccggaatgtt actttgttga
    56281 gccgtttttg ccggttcacg ccagcggacc tgcacatttg cggcgataat tcgtcaccaa
    56341 aagagagaat gtccggcaca ccctaaccaa cccaacgcca gtcttcgccc cctccagtta
    56401 acttatgttt agcccatgcg cccgcaggcg caggcaaaac aactggaggc attgtcgcca
    56461 tatatttcgc cagccagttc atggccagcg cgcgctcaat agtggattgc tcagcggtca
    56521 aaccattaaa atagcggccg tctgggtagc gtcaccaccg ctattgagca gcacaaccgt
    56581 gtgcgccagc ggctgattgc tgtttgccgc tttatcccac gcatattgct gaacctgtgg
    56641 catcaccgtg gacttcaact gcgctttttt cagcaacgtc atggttaaga cgtttgccac
    56701 cgcatctgcc agtggagatt ccggtgtatc gaggatcagg ctgtcattga tatcgcggat
    56761 gtcttcctca gagaaatctt catctttagt tccgcgccgg gcgatagctt catccccccc
    56821 tttcaacaac gttttgcacg gcagattcat ctcctgtgcc cacgccagca ccagcgcccg
    56881 atgcaataac ggcatgttat ccgcctgctc ggcataactg tcgagcatat gctgccagta
    56941 ttccggttgt tgcgttacgc cgagcgcctg gctggcctgc cagtcggcgt accatgccca
    57001 tgccgtaagg aaggcgtcac cattgccatc ttcaccccac caggtaaagc gcgctccggg
    57061 ccccgccagt tgcatcagcc gcagacggtt atcctgaatc atctgacgaa tgtcgttagc
    57121 ggcggcactt tgatgatcgg caagcgaacg ccaggcgaga ctgagcggga tcagacggct
    57181 accggtgttg atcaccccac cccacggttc atcgactaac gcatcaagat tgttgcggaa
    57241 aatctcctgc ggcgtttcac tactttgtag ccggatatta ctcgcctgct cgggcaacat
    57301 cagcgcgttc tcgccaccgc cgagcatgac atttttctgc tgttcaacgg gccagctgtt
    57361 gtccacaaaa gacagttttg tgctaatgct gtcctgcact tgcccatttt gttgcagttc
    57421 tgcacttaac aagccagatt gctgaatatt ctgcgtcagc gaaatataat tcgccccttt
    57481 gtgcagcgtc agcgtctggc gcatctcagc gcctgcaaat ttagtcacca gcgccaccgg
    57541 ttcgttatcc tgctgactga agataaacag tcctgccgcc ggtttgtcgc ccacgcgata
    57601 caccgttggc atactccact tcatgtagag atttttttcc gaacgcagat aagcacgccc
    57661 ctgcccgacc agcccgtcgc cgttcatccc acgcgcggtg atacgccagc gggttaacga
    57721 atcaggcatc aggaacgtga aatacgcttt gccttgttta tcggttgtga gtgacggcat
    57781 ccatgccgcg gtatccacct cttcacgccg tggacgttca agcattttta ctcgccgctc
    57841 gctgcggtta gtcgcgccag gcgcaaccgg ctcgctggag agcgcctggt catagctgat
    57901 aaacgacaga ctggagctgg tacgcacatt gttacgcccc agcggataga aaaatttgcc
    57961 gatattcggc gcgatttctg gttgcagcgc gtagatcatt tcatcgacca cgcctaccgt
    58021 tagctgcgca gaaacaggtt tacctttcag cgacgaggtt aa
//
LOCUS       DAECOD010000013        55431 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.013, whole genome shotgun sequence.
ACCESSION   DAECOD010000013 DAECOD010000000
VERSION     DAECOD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.013"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KKO15_000775"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KKO15_000775"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBD6359681.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KKO15_000776"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KKO15_000776"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBD6359682.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KKO15_000777"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KKO15_000777"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBD6359683.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KKO15_000778"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KKO15_000778"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBD6359684.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KKO15_000779"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KKO15_000779"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBD6359685.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KKO15_000780"
     CDS             7826..9559
                     /locus_tag="KKO15_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBD6359686.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KKO15_000781"
     CDS             9708..10559
                     /locus_tag="KKO15_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6359687.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KKO15_000782"
     CDS             complement(10546..10887)
                     /locus_tag="KKO15_000782"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD6359688.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KKO15_000783"
     CDS             complement(10889..11767)
                     /locus_tag="KKO15_000783"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBD6359689.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="KKO15_000784"
     CDS             complement(11733..14030)
                     /locus_tag="KKO15_000784"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD6359690.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVAGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="KKO15_000785"
     CDS             complement(14081..14401)
                     /locus_tag="KKO15_000785"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD6359691.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KKO15_000786"
     CDS             complement(14416..15495)
                     /locus_tag="KKO15_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBD6359692.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KKO15_000787"
     CDS             15804..16907
                     /locus_tag="KKO15_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBD6359693.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KKO15_000788"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KKO15_000788"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6359694.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KKO15_000789"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KKO15_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KKO15_000790"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KKO15_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KKO15_000791"
     CDS             20366..20983
                     /locus_tag="KKO15_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBD6359695.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KKO15_000792"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KKO15_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBD6359696.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KKO15_000793"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KKO15_000793"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBD6359697.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KKO15_000794"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KKO15_000794"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBD6359698.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KKO15_000795"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KKO15_000795"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBD6359699.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KKO15_000796"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KKO15_000796"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBD6359700.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KKO15_000797"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KKO15_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBD6359701.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KKO15_000798"
     CDS             30130..30738
                     /locus_tag="KKO15_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBD6359702.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KKO15_000799"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KKO15_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBD6359703.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KKO15_000800"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KKO15_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBD6359704.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KKO15_000801"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KKO15_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBD6359705.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KKO15_000802"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KKO15_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBD6359706.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KKO15_000803"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KKO15_000803"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBD6359707.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KKO15_000804"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KKO15_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBD6359708.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KKO15_000805"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KKO15_000805"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBD6359709.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KKO15_000806"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KKO15_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBD6359710.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KKO15_000807"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KKO15_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBD6359711.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KKO15_000808"
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KKO15_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBD6359712.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFSPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            40803..42311
                     /gene="glpK"
                     /locus_tag="KKO15_000809"
     CDS             40803..42311
                     /gene="glpK"
                     /locus_tag="KKO15_000809"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBD6359713.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42541..43551
                     /gene="glpX"
                     /locus_tag="KKO15_000810"
     CDS             42541..43551
                     /gene="glpX"
                     /locus_tag="KKO15_000810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD6359714.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43648..44394
                     /gene="fpr"
                     /locus_tag="KKO15_000811"
     CDS             43648..44394
                     /gene="fpr"
                     /locus_tag="KKO15_000811"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBD6359715.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44399..44827)
                     /gene="uspD"
                     /locus_tag="KKO15_000812"
     CDS             complement(44399..44827)
                     /gene="uspD"
                     /locus_tag="KKO15_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBD6359716.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44854..45153)
                     /locus_tag="KKO15_000813"
     CDS             complement(44854..45153)
                     /locus_tag="KKO15_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBD6359717.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45365..45805)
                     /locus_tag="KKO15_000814"
                     /pseudo
     CDS             complement(45365..45805)
                     /locus_tag="KKO15_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45906..46505
                     /locus_tag="KKO15_000815"
     CDS             45906..46505
                     /locus_tag="KKO15_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBD6359718.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46613..47380
                     /gene="tpiA"
                     /locus_tag="KKO15_000816"
     CDS             46613..47380
                     /gene="tpiA"
                     /locus_tag="KKO15_000816"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBD6359719.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47435..48190)
                     /gene="cdh"
                     /locus_tag="KKO15_000817"
     CDS             complement(47435..48190)
                     /gene="cdh"
                     /locus_tag="KKO15_000817"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBD6359720.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48297..49286)
                     /gene="sbp"
                     /locus_tag="KKO15_000818"
     CDS             complement(48297..49286)
                     /gene="sbp"
                     /locus_tag="KKO15_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBD6359721.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49606..50568)
                     /gene="pfkA"
                     /locus_tag="KKO15_000819"
     CDS             complement(49606..50568)
                     /gene="pfkA"
                     /locus_tag="KKO15_000819"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBD6359722.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50749..51651)
                     /gene="fieF"
                     /locus_tag="KKO15_000820"
     CDS             complement(50749..51651)
                     /gene="fieF"
                     /locus_tag="KKO15_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBD6359723.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51799..52299)
                     /gene="cpxP"
                     /locus_tag="KKO15_000821"
     CDS             complement(51799..52299)
                     /gene="cpxP"
                     /locus_tag="KKO15_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBD6359724.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52449..53147
                     /gene="cpxR"
                     /locus_tag="KKO15_000822"
     CDS             52449..53147
                     /gene="cpxR"
                     /locus_tag="KKO15_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBD6359725.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53144..54517
                     /gene="cpxA"
                     /locus_tag="KKO15_000823"
     CDS             53144..54517
                     /gene="cpxA"
                     /locus_tag="KKO15_000823"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBD6359726.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54623..55297)
                     /gene="yiiM"
                     /locus_tag="KKO15_000824"
     CDS             complement(54623..55297)
                     /gene="yiiM"
                     /locus_tag="KKO15_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBD6359727.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13496 a        15023 c        14169 g        12743 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccagtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgt caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccagtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccagccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggttatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccacacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg
    40861 tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct
    40921 acccaaaacc aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca
    40981 cgctggtaga agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta
    41041 ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata
    41101 acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg
    41161 gtttagaaga ttatatccgc agcaataccg gtctggtgat tgacccgtac ttttctggca
    41221 ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg
    41281 aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc
    41341 atgtgaccga ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact
    41401 gggacgacaa aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc
    41461 gttcttccga agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa
    41521 tctccgggat cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag
    41581 ggatggcgaa gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag
    41641 cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga
    41701 actatgcgtt ggaaggtgcg gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg
    41761 agatgaagtt gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca
    41821 ccaatggtgt gtatgtggtt ccggcattta ccgggctggg tgcgccgtac tgggacccgt
    41881 atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg
    41941 cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact
    42001 ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga
    42061 tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca
    42121 ccgcattggg tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg
    42181 agctgcaaga gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc
    42241 gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac
    42301 acgacgagta atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc
    42361 ttacgcgtct tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc
    42421 acgccgcatc cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca
    42481 ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct
    42541 atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc
    42601 tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg
    42661 cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc
    42721 gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta
    42781 gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg
    42841 ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg
    42901 gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg
    42961 gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg
    43021 attctggcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc
    43081 gtatttgcta ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc
    43141 gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg
    43201 atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc
    43261 gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc
    43321 atcgaagccg gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc
    43381 tctgccaccg gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc
    43441 gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc
    43501 atccactatc tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga
    43561 tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag
    43621 gcaagtcaat caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa
    43681 gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt
    43741 accgccgggc aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc
    43801 tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc
    43861 gatggcaaat taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt
    43921 agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg
    43981 ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta
    44041 gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat
    44101 ttgccactga tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg
    44161 gtcagtcggg aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt
    44221 ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg
    44281 tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg
    44341 acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt
    44401 acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat
    44461 atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact
    44521 gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt
    44581 ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct
    44641 tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta
    44701 agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg
    44761 ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat
    44821 aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc
    44881 aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc
    44941 ctccagatcc ttaatcttat aggccacgct ggccccttca tctgcagagg atgcagcggc
    45001 ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg
    45061 cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc
    45121 tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct
    45181 gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta
    45241 tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta
    45301 ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta
    45361 aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat
    45421 atttattttc gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa
    45481 tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt
    45541 tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc
    45601 gatcatgcaa gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa
    45661 acgccgcggt ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc
    45721 ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg
    45781 agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct
    45841 acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac
    45901 tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa
    45961 caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag
    46021 cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca
    46081 gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc
    46141 gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct
    46201 taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag
    46261 cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga
    46321 ccaaaacaca aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt
    46381 attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc
    46441 tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata
    46501 aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg
    46561 ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca
    46621 tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt
    46681 ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc
    46741 ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc
    46801 gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct
    46861 gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa
    46921 agagtctgac gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc
    46981 ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg
    47041 cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt
    47101 tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc
    47161 acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga
    47221 acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc
    47281 tcagccagac atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc
    47341 agtgatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt
    47401 catatccggc acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat
    47461 cctggatttc ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca
    47521 acacaaacga attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc
    47581 gcgcctcagg cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt
    47641 ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc
    47701 ggctgctgat gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag
    47761 agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa
    47821 ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg
    47881 ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa
    47941 tacgatacgt tggcatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt
    48001 atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg
    48061 gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc
    48121 agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg
    48181 cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga
    48241 caaataaaaa acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag
    48301 cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc
    48361 cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac
    48421 tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct
    48481 tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg
    48541 accactttat cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg
    48601 atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg
    48661 atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa
    48721 tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg
    48781 ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca
    48841 gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca
    48901 tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg
    48961 gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg
    49021 tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac
    49081 gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc
    49141 tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca
    49201 tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat
    49261 gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca
    49321 aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg
    49381 ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc
    49441 gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt
    49501 caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc
    49561 tgtcatcggt ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt
    49621 cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat
    49681 gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag
    49741 cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg
    49801 agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt
    49861 ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc
    49921 gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa
    49981 ttcaacttcc ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag
    50041 atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt
    50101 gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt
    50161 gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc
    50221 catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc
    50281 gtcgatacca cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa
    50341 ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct
    50401 gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc
    50461 cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat
    50521 gcctggcgca tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga
    50581 actttggaat gcaaaatgaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca
    50641 acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat
    50701 ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat
    50761 agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga
    50821 tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac
    50881 caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg
    50941 ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat
    51001 ttcttgccgt tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc
    51061 catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc
    51121 gcgatgccag ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac
    51181 atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac
    51241 ccaccgctga aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac
    51301 cccgacgcct ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa
    51361 caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc
    51421 tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag
    51481 taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact
    51541 caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc
    51601 cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc
    51661 cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg
    51721 caaattgagg ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg
    51781 caaggaaaac agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga
    51841 tgaacttttt tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc
    51901 gtttaaaacc gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc
    51961 catctcaacc tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc
    52021 gttttcatca aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt
    52081 aacaggaggc tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg
    52141 ttcggttaaa cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc
    52201 acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc
    52261 cagcgttgag gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc
    52321 tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt
    52381 aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta
    52441 tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa
    52501 aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc
    52561 ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa
    52621 atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga
    52681 cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc
    52741 tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat tcgcgcaatc ctgcgccgtt
    52801 cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg
    52861 ccttagtgct gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa
    52921 ccggtactga gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc
    52981 gtgaacattt aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg
    53041 atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt
    53101 ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac
    53161 cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat
    53221 gttacccaag ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg
    53281 gctgatgatt gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat
    53341 gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt
    53401 attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg
    53461 taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt
    53521 ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat
    53581 tcgtccggcc agtagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt
    53641 actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct
    53701 ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg
    53761 ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa
    53821 ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat
    53881 ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg
    53941 tcgtagcggt gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag
    54001 catgatcaac gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga
    54061 aaccatcaaa gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga
    54121 gcaaatgggc aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa
    54181 tccgaacgcc ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca
    54241 tacgaagatt gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga
    54301 cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac
    54361 cgatgaagca cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac
    54421 cgccattcag cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg
    54481 gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata
    54541 aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct
    54601 tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct
    54661 tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg
    54721 agagtaaacg gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct
    54781 cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt
    54841 tccctggcgc tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg
    54901 ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga
    54961 cctgaattaa tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga
    55021 gaccgtcggt tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg
    55081 gaaattcccg cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg
    55141 gcccgccgtg aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca
    55201 tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct
    55261 gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt
    55321 tccccattag accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg
    55381 caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg a
//
LOCUS       DAECOD010000014        55324 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.014, whole genome shotgun sequence.
ACCESSION   DAECOD010000014 DAECOD010000000
VERSION     DAECOD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.014"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(101..1765)
                     /gene="glnS"
                     /locus_tag="KKO15_000825"
     CDS             complement(101..1765)
                     /gene="glnS"
                     /locus_tag="KKO15_000825"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBD6359728.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1968..3914)
                     /gene="nagE"
                     /locus_tag="KKO15_000826"
     CDS             complement(1968..3914)
                     /gene="nagE"
                     /locus_tag="KKO15_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBD6359729.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4247..5047
                     /gene="nagB"
                     /locus_tag="KKO15_000827"
     CDS             4247..5047
                     /gene="nagB"
                     /locus_tag="KKO15_000827"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD6359730.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5107..6255
                     /gene="nagA"
                     /locus_tag="KKO15_000828"
     CDS             5107..6255
                     /gene="nagA"
                     /locus_tag="KKO15_000828"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD6359731.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6264..7484
                     /gene="nagC"
                     /locus_tag="KKO15_000829"
                     /gene_synonym="nagR"
     CDS             6264..7484
                     /gene="nagC"
                     /locus_tag="KKO15_000829"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBD6359732.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7532..8284
                     /gene="nagD"
                     /locus_tag="KKO15_000830"
     CDS             7532..8284
                     /gene="nagD"
                     /locus_tag="KKO15_000830"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBD6359733.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8681..10345
                     /gene="asnB"
                     /locus_tag="KKO15_000831"
     CDS             8681..10345
                     /gene="asnB"
                     /locus_tag="KKO15_000831"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBD6359734.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10724..10800
                     /locus_tag="KKO15_000832"
     tRNA            10724..10800
                     /locus_tag="KKO15_000832"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10758..10760,aa:Met,seq:cat)
     gene            10809..10893
                     /locus_tag="KKO15_000833"
     tRNA            10809..10893
                     /locus_tag="KKO15_000833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10843..10845,aa:Leu,seq:tag)
     gene            10917..10991
                     /locus_tag="KKO15_000834"
     tRNA            10917..10991
                     /locus_tag="KKO15_000834"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10949..10951,aa:Gln,seq:ttg)
     gene            11026..11100
                     /locus_tag="KKO15_000835"
     tRNA            11026..11100
                     /locus_tag="KKO15_000835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11058..11060,aa:Gln,seq:ttg)
     gene            11116..11192
                     /locus_tag="KKO15_000836"
     tRNA            11116..11192
                     /locus_tag="KKO15_000836"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11150..11152,aa:Met,seq:cat)
     gene            11241..11315
                     /locus_tag="KKO15_000837"
     tRNA            11241..11315
                     /locus_tag="KKO15_000837"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11273..11275,aa:Gln,seq:ctg)
     gene            11353..11427
                     /locus_tag="KKO15_000838"
     tRNA            11353..11427
                     /locus_tag="KKO15_000838"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11385..11387,aa:Gln,seq:ctg)
     gene            complement(11581..12756)
                     /gene="ubiF"
                     /locus_tag="KKO15_000839"
     CDS             complement(11581..12756)
                     /gene="ubiF"
                     /locus_tag="KKO15_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBD6359735.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12902..14326
                     /gene="miaB"
                     /locus_tag="KKO15_000840"
     CDS             12902..14326
                     /gene="miaB"
                     /locus_tag="KKO15_000840"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBD6359736.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14479..15519
                     /locus_tag="KKO15_000841"
                     /pseudo
     CDS             14479..15519
                     /locus_tag="KKO15_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            15516..15983
                     /gene="ybeY"
                     /locus_tag="KKO15_000842"
     CDS             15516..15983
                     /gene="ybeY"
                     /locus_tag="KKO15_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBD6359737.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16073..16951
                     /gene="corC"
                     /locus_tag="KKO15_000843"
     CDS             16073..16951
                     /gene="corC"
                     /locus_tag="KKO15_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBD6359738.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16976..18514
                     /gene="lnt"
                     /locus_tag="KKO15_000844"
     CDS             16976..18514
                     /gene="lnt"
                     /locus_tag="KKO15_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBD6359739.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18911..19819
                     /gene="gltI"
                     /locus_tag="KKO15_000845"
     CDS             18911..19819
                     /gene="gltI"
                     /locus_tag="KKO15_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBD6359740.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19989..20729
                     /gene="gltJ"
                     /locus_tag="KKO15_000846"
     CDS             19989..20729
                     /gene="gltJ"
                     /locus_tag="KKO15_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBD6359741.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20729..21403
                     /gene="gltK"
                     /locus_tag="KKO15_000847"
     CDS             20729..21403
                     /gene="gltK"
                     /locus_tag="KKO15_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBD6359742.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21403..22128
                     /gene="gltL"
                     /locus_tag="KKO15_000848"
     CDS             21403..22128
                     /gene="gltL"
                     /locus_tag="KKO15_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBD6359743.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22246..23181
                     /gene="rihA"
                     /locus_tag="KKO15_000849"
     CDS             22246..23181
                     /gene="rihA"
                     /locus_tag="KKO15_000849"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBD6359744.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23265..24935
                     /gene="hscC"
                     /locus_tag="KKO15_000850"
                     /pseudo
     CDS             23265..24935
                     /gene="hscC"
                     /locus_tag="KKO15_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(24995..26446)
                     /gene="djlC"
                     /locus_tag="KKO15_000851"
     CDS             complement(24995..26446)
                     /gene="djlC"
                     /locus_tag="KKO15_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBD6359745.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26443..27150)
                     /locus_tag="KKO15_000852"
     CDS             complement(26443..27150)
                     /locus_tag="KKO15_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6359746.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27252..27806
                     /locus_tag="KKO15_000853"
     CDS             27252..27806
                     /locus_tag="KKO15_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD6359747.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27816..29243)
                     /locus_tag="KKO15_000854"
     CDS             complement(27816..29243)
                     /locus_tag="KKO15_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBD6359748.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29240..29947)
                     /locus_tag="KKO15_000855"
     CDS             complement(29240..29947)
                     /locus_tag="KKO15_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6359749.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30111..31088
                     /locus_tag="KKO15_000856"
     CDS             30111..31088
                     /locus_tag="KKO15_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD6359750.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31158..31640)
                     /locus_tag="KKO15_000857"
     CDS             complement(31158..31640)
                     /locus_tag="KKO15_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBD6359751.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31875..34457
                     /gene="leuS"
                     /locus_tag="KKO15_000858"
     CDS             31875..34457
                     /gene="leuS"
                     /locus_tag="KKO15_000858"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBD6359752.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34472..35053
                     /gene="lptE"
                     /locus_tag="KKO15_000859"
     CDS             34472..35053
                     /gene="lptE"
                     /locus_tag="KKO15_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBD6359753.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            35053..36084
                     /gene="holA"
                     /locus_tag="KKO15_000860"
     CDS             35053..36084
                     /gene="holA"
                     /locus_tag="KKO15_000860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBD6359754.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36086..36727
                     /gene="nadD"
                     /locus_tag="KKO15_000861"
     CDS             36086..36727
                     /gene="nadD"
                     /locus_tag="KKO15_000861"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBD6359755.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36751..37362
                     /locus_tag="KKO15_000862"
     CDS             36751..37362
                     /locus_tag="KKO15_000862"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBD6359756.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37622..37939
                     /gene="rsfS"
                     /locus_tag="KKO15_000863"
                     /gene_synonym="rsfA"
     CDS             37622..37939
                     /gene="rsfS"
                     /locus_tag="KKO15_000863"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBD6359757.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            37943..38410
                     /gene="rlmH"
                     /locus_tag="KKO15_000864"
     CDS             37943..38410
                     /gene="rlmH"
                     /locus_tag="KKO15_000864"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBD6359758.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38441..40342
                     /gene="mrdA"
                     /locus_tag="KKO15_000865"
     CDS             38441..40342
                     /gene="mrdA"
                     /locus_tag="KKO15_000865"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBD6359759.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPYEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40345..41457
                     /gene="mrdB"
                     /locus_tag="KKO15_000866"
                     /gene_synonym="rodA"
     CDS             40345..41457
                     /gene="mrdB"
                     /locus_tag="KKO15_000866"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBD6359760.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41468..42556
                     /gene="rlpA"
                     /locus_tag="KKO15_000867"
     CDS             41468..42556
                     /gene="rlpA"
                     /locus_tag="KKO15_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBD6359761.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42695..43906
                     /gene="dacA"
                     /locus_tag="KKO15_000868"
     CDS             42695..43906
                     /gene="dacA"
                     /locus_tag="KKO15_000868"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBD6359762.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            44017..44280
                     /locus_tag="KKO15_000869"
     CDS             44017..44280
                     /locus_tag="KKO15_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBD6359763.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44381..45022
                     /gene="lipB"
                     /locus_tag="KKO15_000870"
     CDS             44381..45022
                     /gene="lipB"
                     /locus_tag="KKO15_000870"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBD6359764.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            45281..46234
                     /locus_tag="KKO15_000871"
     CDS             45281..46234
                     /locus_tag="KKO15_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD6359765.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            46443..47408
                     /gene="lipA"
                     /locus_tag="KKO15_000872"
     CDS             46443..47408
                     /gene="lipA"
                     /locus_tag="KKO15_000872"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBD6359766.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(47509..47712)
                     /gene="tatE"
                     /locus_tag="KKO15_000873"
     CDS             complement(47509..47712)
                     /gene="tatE"
                     /locus_tag="KKO15_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBD6359767.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(47841..48629)
                     /locus_tag="KKO15_000874"
     CDS             complement(47841..48629)
                     /locus_tag="KKO15_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBD6359768.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            48722..49105
                     /gene="crcB"
                     /locus_tag="KKO15_000875"
     CDS             48722..49105
                     /gene="crcB"
                     /locus_tag="KKO15_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBD6359769.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(49159..49368)
                     /gene="cspE"
                     /locus_tag="KKO15_000876"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(49159..49368)
                     /gene="cspE"
                     /locus_tag="KKO15_000876"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD6359770.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(49543..50103)
                     /gene="pagP"
                     /locus_tag="KKO15_000877"
     CDS             complement(49543..50103)
                     /gene="pagP"
                     /locus_tag="KKO15_000877"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBD6359771.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            50692..52077
                     /gene="dcuC"
                     /locus_tag="KKO15_000878"
     CDS             50692..52077
                     /gene="dcuC"
                     /locus_tag="KKO15_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD6359772.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(52118..52459)
                     /locus_tag="KKO15_000879"
                     /pseudo
     CDS             complement(52118..52459)
                     /locus_tag="KKO15_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(52797..53306)
                     /locus_tag="KKO15_000880"
     CDS             complement(52797..53306)
                     /locus_tag="KKO15_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6359773.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            53469..53714
                     /locus_tag="KKO15_000881"
     CDS             53469..53714
                     /locus_tag="KKO15_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6359774.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            53689..55320
                     /locus_tag="KKO15_000882"
                     /pseudo
     CDS             53689..55320
                     /locus_tag="KKO15_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        13891 a        13931 c        13837 g        13665 t
ORIGIN      
        1 gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt tattgccgga
       61 tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct actttcgccc
      121 aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg gaatggcggc
      181 tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca accgcatctt
      241 tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta atcaccgaca
      301 ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac aaacggattt
      361 caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact ttacgaccat
      421 ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattac
      481 cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc agacgcactt
      541 ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat
      601 cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt ttgttcggat
      661 ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg
      721 gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg atgcaggatt
      781 ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact
      841 cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg
      901 ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca
      961 tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga acaggaatgg
     1021 tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca agcgtacaca
     1081 gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg tacatcgggt
     1141 agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg tacagcaccg
     1201 gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg caggctttac
     1261 cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct tcaacgctgc
     1321 ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat tcgcggatct
     1381 gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg
     1441 cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta
     1501 accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt accgggttag
     1561 tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc
     1621 agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt
     1681 gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg ataaagttag
     1741 tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg
     1801 cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat tgataatcta
     1861 acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct ccaccgcttg
     1921 tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta ctttttgatt
     1981 tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag caagccactg
     2041 aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt agcgttcagg
     2101 taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc ctcttccacc
     2161 agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg gacgacgatc
     2221 tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat tttcacgatt
     2281 gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac accgtcaccc
     2341 accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac atcaccggta
     2401 atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg ttttgctaca
     2461 ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc acgggcaacg
     2521 actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac ctgaatagtc
     2581 tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt atcgttgacg
     2641 cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat cgctttcagg
     2701 ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag accttcttca
     2761 gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg acccggcgtt
     2821 ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc gaagaagata
     2881 acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt atacatcaac
     2941 gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag cagcgttgcc
     3001 acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg cgcaaggaac
     3061 atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac agcaacagaa
     3121 agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta catcgccagc
     3181 gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa catccccgcg
     3241 gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc cgccgcgttg
     3301 gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag accggttggg
     3361 atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc agaaacgatc
     3421 cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc aaaaatggcc
     3481 gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc gccgaagaag
     3541 ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc accaaccaga
     3601 ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt caccatcgct
     3661 ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc gctgtctttc
     3721 gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa aattgcaccg
     3781 cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg cagcaacagc
     3841 gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa
     3901 cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt aaagccgtat
     3961 ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta gatcatctgc
     4021 tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt ttgtgagttt
     4081 tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa acttatttta
     4141 tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt acgcttaaag
     4201 atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga gactgatccc
     4261 cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa
     4321 tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg gcggcacgcc
     4381 gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca gctttaagca
     4441 cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc cggaaagcta
     4501 ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag aaaacatcaa
     4561 ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg aagaaaaaat
     4621 ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg gtcatattgc
     4681 atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc tgactcatga
     4741 cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc caaaatatgc
     4801 cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta tggtgctggg
     4861 tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc atatgtggac
     4921 catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac cttccaccat
     4981 ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa atatcaaagg
     5041 tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt aaaatcgggg
     5101 gtcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt tcttgatgac
     5161 cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc ggaactgccg
     5221 ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt tatcgatgtg
     5281 cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt cagcgtggaa
     5341 acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta tctgccgacg
     5401 cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg tgagtacctg
     5461 gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct gaatctggta
     5521 aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc
     5581 ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat ggttcctgcg
     5641 gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca ctccaacgcg
     5701 acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac ccatctgtac
     5761 aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa
     5821 gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc caacattcgt
     5881 aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac cgcgccagca
     5941 ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg taacggactt
     6001 tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga aggcgtgcgt
     6061 aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc gacgctctat
     6121 ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg taaagtagcc
     6181 aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa cggtaacgag
     6241 gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata ggtaatgttg
     6301 atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag tacgggccaa
     6361 tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta accaaaatta
     6421 cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc tccaccgggg
     6481 gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc
     6541 ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg ctggcagaag
     6601 aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg ctgaatgcca
     6661 ttgctcagtt tattgatagc taccagcgta agctgcgcga gctaatcgcc atttcggtga
     6721 tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg catattcagg
     6781 tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc tgtttcgttg
     6841 gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt caggattgcg
     6901 aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc tctaacgggc
     6961 gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag gtcgaaccgc
     7021 tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc gccaacgctg
     7081 ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt gtgccactgg
     7141 acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc ctggcctcgg
     7201 aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct atcaacttat
     7261 ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa gtgctgctcc
     7321 ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact aatctgccgg
     7381 tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg gtaaaacgcg
     7441 ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct tttatagtgg
     7501 cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa tttgcgatat
     7561 cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat ttttgcacgg
     7621 gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc agactgggca
     7681 agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg tgttttatac
     7741 ctctgcgatg gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag cgtatgtggt
     7801 gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta ccgatgtgaa
     7861 ccctgatttc gtgattgttg gcgaaacacg ttcttacaac tgggacatga tgcataaagc
     7921 agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca cccacgggcg
     7981 cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct ccgggcgcaa
     8041 accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa acaaaatgca
     8101 ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata ttctggcggg
     8161 cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc tcgacgatat
     8221 cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa tcgacgttat
     8281 ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc aaactgcctg
     8341 atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt catgcttttg
     8401 taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt tatcagcaat
     8461 ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg attcatcaat
     8521 aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta cgttttttat
     8581 ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt taaacaagca
     8641 aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt
     8701 attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc tgtcacgcct
     8761 gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg ccattctcgc
     8821 tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca
     8881 aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat tgcgcgctga
     8941 atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc tcgcgctgta
     9001 tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgccctgta
     9061 cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca tcccactgta
     9121 tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat cgctggtgcc
     9181 agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc aggacggcga
     9241 aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag ataacgtgac
     9301 cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc tgatgtctga
     9361 tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt ccgctatcac
     9421 caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca
     9481 gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag cccaggaagt
     9541 ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg aaggtctgga
     9601 tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta ttcgcgcttc
     9661 aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc
     9721 cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag cgcccaacgc
     9781 taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt atgactgcgc
     9841 gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa
     9901 aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg gtaacggcaa
     9961 aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa gcgtggcctg
    10021 gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga
    10081 agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc gcttcccgta
    10141 caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag aactgttccc
    10201 acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt cttcagctaa
    10261 agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc gtgcggttgg
    10321 cgttcaccag tcggcgtata agtaagaaat gcggtacagc cccggagaaa tccggggctg
    10381 ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc
    10441 cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta atttcccacc
    10501 actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta gggtggggaa
    10561 aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa cctaaccaaa
    10621 cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc tctatacgca
    10681 taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa gatggctacg tagctcagtt
    10741 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    10801 tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa
    10861 ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg tacggatggg
    10921 gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg
    10981 taccccagcc atcttcttcg agtaagcggt tcaccgcccg gttattgggg tatcgccaag
    11041 cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca
    11101 tcgaagaaat aatctggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    11161 ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa tacgttcggc
    11221 aaattcaaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga ttctgattcc
    11281 ggcattccga ggttcgaatc ctcgtacccc agccaattta ttcaagacgc ttacatcgta
    11341 agtgcaccca gttggggtat cgccaagcgg taaggcaccg gattctgatt ccggcattcc
    11401 gaggttcgaa tcctcgtacc ccagccacat taaaaaagct cgcttcggcg agctttttgc
    11461 ttttctgcgt tcattcaatg tcgaatgcga tgttgacacg tcttatcctt caatgtcgga
    11521 tgcgacgctg ccgcgtctta tccgacctac ggctggcacg catccggcaa tgttgtaagg
    11581 ctacaaccct aacgcatatt tcagcgcctg acgtttcaac acgccagcac gctctgccgc
    11641 cattaacccg agattacgca caaaacgcag cggcggcaga ttattactga atccggcata
    11701 aaacagatcc ataccacttt gcataatgaa gttatccgcc atgcgccgca tctggtaacg
    11761 cttgaggaca ggataactgg cccacgcttc accgtagctg cgggcattca ccagaacatc
    11821 aatcagggca tcgacatcac gataaccaag attcaccccc tgccccgcca gcggatggat
    11881 ggtatgcgcg gcatcgccca ccagcgcaag ccccggctgc acatactgca acgcatgtcg
    11941 gcgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg taacccagac gcgacgggaa
    12001 atgcttcgcg atttccgctt ggagttgcgc catattcata ttctgcaact ggcgaatacg
    12061 cgccggagag tcataccaca ccagcgatgc ccagttatca aacaacggca gaaacgcacg
    12121 cggtccgtcc ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct cacactgtac
    12181 gctaatcaac atgcacgact gcgcatactg ccaggcatga acgccaattc ccgccatctg
    12241 ccgcacctga gaatttgcgc cgtcggcacc aatcaccagt ttcgcgcgga tcgtttcacc
    12301 gcccttcagt tccagctcct gaagatcatt atctcgatgt aatgcaatca gcgaggttgg
    12361 cacacgtaac gttactttcg gatgcgcttc cagcgcctgc cacaacgcct gttgcaggac
    12421 tgtgttttcc accatgtagc caagcaacgg caactttaat tcggctgcgt caaataccac
    12481 atgtgccgtt tcccactccc aggtttccag tctgcggtaa ggatggcaac gcatagcctg
    12541 tactgcatcc cagaccccta accctttaag caatgatacc gaagccgcgc tgatcgccga
    12601 gatccgcacg tccggttggc tgtcagcgac aaacggtgca ggttctgcgt gctcgatcac
    12661 cgttaccgaa aatccgtgct gtgccagccc cagcgccagt gcgccgccga ccattcctcc
    12721 gccgacaatg gcaatttccg ttggttgatt tgtcatggtc aatcatcctg ttagcaatat
    12781 gctttaagtt tacaggattt ttacccccaa gggctgataa cgctcatact ggtcacaacg
    12841 gcagcaaagc attacactat gcgccctgca ttcctggcta ctatttcgca agagcaagtc
    12901 gatgaccaaa aaactccata ttaaaacctg gggctgtcag atgaacgagt acgattcatc
    12961 gaagatggcc gatctgctgg atgccaccca cggctatcaa ctgaccgacg tggcggaaga
    13021 agcggatgtg ctactgctga acacctgctc aatccgcgag aaggctcagg aaaaagtctt
    13081 ccatcagttg ggtcgctgga aactgttaaa agagaagaat ccagacctga ttatcggcgt
    13141 cggtggctgc gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc actatgtcga
    13201 tattattttt gggccgcaaa cgctgcaccg tctgccggag atgatcaact ccgtgcgcgg
    13261 cgaccgcagc ccggttgtag atatcagctt cccggaaatc gagaagtttg accgtctgcc
    13321 ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc atggaaggct gcaataaata
    13381 ttgcacctac tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc cgtctgacga
    13441 tattctgttt gagattgccc agcttgcggc tcagggcgtg cgtgaagtca acctgctcgg
    13501 tcagaatgtg aatgcctggc gtggtgagaa ctacgacggc accaccggat cgtttgccga
    13561 tctgttgcgt ctggttgctg cgatcgacgg gatcgatcgt attcgcttta ccaccagcca
    13621 tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc gacacgcctg agctggtgag
    13681 cttcctgcat ctgccggtac agagcggttc cgatcgcatt ctgaacctga tgggacgtac
    13741 ccatacggcg ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc gtccggatat
    13801 tcagatcagt tctgacttca ttgttggctt ccctggcgaa accaccgaag acttcgagaa
    13861 aacgatgaag ctgattgccg acgtcaattt cgacatgagc tacagcttta tcttctctgc
    13921 acgtccagga acgcctgccg ccgatatggt tgatgatgtt ccggaagaag agaagaagca
    13981 gcgtctgtat attctgcaag aacgtatcaa tcagcaagcg atggcatgga gccgtcggat
    14041 gctcggcacc acccagcgta ttctggtaga agggacttcg cgtaagagca tcatggagct
    14101 ttccgggcgt accgaaaata accgcgtggt caacttcgag ggcacaccgg atatgatcgg
    14161 taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg gtaaagtggt
    14221 gcgaactgaa gatgaaatgg gtctgcgcat ggcagaaaca ccggagtcgg tgattgcccg
    14281 cacccgcaaa gaaaacgacc ttggcgtggg ttattatcag ccgtaattct caggcctgcc
    14341 gttccggtgg gccttgtttt ttctctcttt atccccatct ttatcgcaat gcttgcgcct
    14401 ttttcctgtg gcgtgaataa tttccttata ggccggtgaa tgttcagctt ccggcggcat
    14461 acagataaga ggaacggttt gaacatagac actcgcgaaa tcaccctgga gccagcagac
    14521 aatgcgcgtc tgttgagcct gtgcggcccg tttgatgaca acatcaagca gctcgaacgc
    14581 cgtctcggca tcgagatcaa tcgccgcgat aaccacttta aactgaccgg ccgtccgatt
    14641 tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg tcgatactgc cccgatgcgc
    14701 ggtcagattc aggatatcga accggaatag atccaccttg cgattaaaga agcgcgggta
    14761 ctggagcaaa gcgcggagag cgtgccggag tacggcaaag cggtcaatat caaaactaaa
    14821 cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt acatcgccaa tattctcgac
    14881 catgacatta ccttcggcgt tggcccggcg ggtacgggta aaacctacct ggcagtggct
    14941 gcggcagttg atgccctgga gcgtcaggaa attcgccgta ttctgctgac tcgtccggcg
    15001 gtcgaagccg gtgagaaact gggcttcctg cctggcgatt taagccagaa agtagacccg
    15061 tatctgcgcc cactgtacga cgcgctgttt gaaatgctgg gctttgagaa agtcgagaaa
    15121 ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg tcgtacgctg
    15181 aacgacgcgt ttatcattct cgatgagagc cagaacacta ccatcgaaca gatgaagatg
    15241 ttcctgaccc gtatcggttt taactcaaaa gcggttatca ccggcgacgt cacacagatc
    15301 gacctgccgc gtaatactaa atcaggctta cgtcatgcca ttgaagtgct ggccgatgtc
    15361 gaagagatca gctttaactt cttccacagc gaagacgtgg ttcgtcaccc ggtggtggcg
    15421 cgtatcgtta acgcttatga agcctgggaa gaagcagaac aaaaacgtaa agcggcgctg
    15481 gcagcagaac gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc ctcgatttac
    15541 aactggcatg tgaagataat tccgggttac cggaagagag ccagtttcaa acatggctga
    15601 atgcggtgat cccgcagttt caggaagagt cggaagtgac tattcgcgtg gtcgataccg
    15661 ccgaaagcca cagtctgaat ctgacctatc gcggtaagga taagccgacc aacgtgctct
    15721 ccttcccgtt tgaagtgccg cctggcatgg agatgtcgct actgggcgat ctggttatct
    15781 gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa accactggag gcgcactggg
    15841 cgcatatggt ggtgcacggc agtctgcatt tgttaggtta cgatcacatt gaagatgacg
    15901 aagcagaaga aatggaagcc ctcgaaacag agattatgct tgctctgggc tatgaggatc
    15961 cgtacattgc cgagaaagaa taatttgcca ggcatttgac tggcagacgt ccccgccgtt
    16021 ggtgattaac gacggcgttt tgattaacta acatgagaac ccatacgacg ccatgagcga
    16081 cgacaattca cacagtagtg acacgataag caacaagaag ggatttttct ccctgttact
    16141 cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc tgatccgtga
    16201 ttccgggcag aacgacctta tcgacgaaga tacgcgcgat atgctcgaag gggtgatgga
    16261 catcgcagac caacgcgttc gcgacatcat gatcccccgc tcccagatga ttaccctgaa
    16321 acgcaaccag acgctggacg aatgccttga tgtcatcatc gagtccgccc actcacgttt
    16381 cccggtgatt agcgaagaca aagatcacat tgaagggatt ctgatggcga aagacttgct
    16441 gccgtttatg cgcagcgatg ctgaagcctt cagcatggac aaagtgttac gtcaggcggt
    16501 tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct ctcagcgtta
    16561 ccacatggcg atcgttattg acgaattcgg tggggtttcc ggtctggtga ccattgaaga
    16621 catcctggaa ctgattgttg gtgagattga agacgagtat gacgaagaag atgatatcga
    16681 cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca ttgaagactt
    16741 caacgaagcg ttcggcaccc actttagcga tgaagaagtc gacactatcg gtggtctggt
    16801 gatgcaggcg tttgggcatc ttccggcgcg tggcgaaact atcgacatcg acggttacca
    16861 gttcaaagtg gcgatggccg atagtcggcg tattattcag gttcatgtca aaatcccgga
    16921 tgactcaccc cagccaaagc tggatgaata aaaccgaaac tggatagata actacatggc
    16981 ttttgcctca ttaattgaac gccagcgcat tcgcctgctg ctggcgttat tattcggtgc
    17041 ctgcggaacg ctggccttct ctccttacga cgtctggcct gcggcgatta tttcgctgat
    17101 ggggcttcag gcgttgacct ttaaccgtcg cccactccag tctgccgcta ttggcttttg
    17161 ctggggattt ggcctctttg gcagcggtat taactgggtc tatgtcagca tcgcgacctt
    17221 tggcgggatg cctggcccgg ttaacatctt cctggtggtg ctgctggcgg cgtatttgtc
    17281 gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa ccacctggct
    17341 gcgcgtggcg attgccaccc ctgctctctg gcaagtgacc gagtttctgc gcggttgggt
    17401 gctgactggc ttcccgtggt tacagttcgg ctatagccag attgatggcc cgttaaaagg
    17461 gctggcaccg ctaatgggcg tggaagccat taacttcctg ctgatgatgg ttagtggcct
    17521 gctggcactg gcgttggtca aacgcaactg gcgtccgctg gtggtggccg tcgtgctgtt
    17581 tgccctaccc ttcccgctgc gttacatcca atggtttacc ccgcaaccgg agaaaaccat
    17641 tcaggtttcg atggttcagg gcgatattcc gcaatcgctg aaatgggacg aaggccagct
    17701 tcttaatacg ctaaagattt actacaacgc aacggcaccg ctgatgggca aatcatcgtt
    17761 gattatctgg ccggagtcgg cgataaccga tctggaaatt aatcagcaac cgttcctcaa
    17821 agcactggac ggtgagttgc gtgataaagg tagctcgctg gtaaccggga ttgtcgacgc
    17881 gcgtctcaat aagcagaacc gctacgatac ctacaacacc atcatcacgc tgggtaaagg
    17941 tgcaccgtac agctacgaat cagccgatcg ctataacaaa aaccatctgg tgccgtttgg
    18001 cgagtttgtc ccgctggagt cgattctgcg tccgttagca ccgttctttg atctgccgat
    18061 gtcgtcgttc agccgtgggc catatatcca gccgccgctg tcggcaaatg gtattgagct
    18121 tactgcggct atttgctacg agatcattct cgccgagcaa gtgcgcgata acttccgccc
    18181 ggataccgac tacctgctga ctatctccaa cgatgcgtgg tttggtaaat ctattggtcc
    18241 atggcaacac ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc cactgttgcg
    18301 cagcaccaac aacggcatta cggcggtgat tggcccgcag ggtgagattc aggcgatgat
    18361 cccgcagttc gcccgcgagg tgttaaccac taacgtgacg ccgaccaccg gactcacacc
    18421 atacgcacgt accggcaact ggccgctgtg ggtgctgacg gcactgtttg gttttgctgc
    18481 tgtgttgatg agtctgcgtc agcgacgtaa ataatccctt cttatcgtgc cggaatacat
    18541 cccatatttc ggcacgctca ccggatgcgg catcagtgca ttatccggat aacaatattc
    18601 tcctatccaa ctttggcaca tctattgctt tgttatacaa ggcaaagctt gaaccagcat
    18661 cagtacaacc cggttgcacc acagcaatcc accggtagca ccaaaagagt gcattttaag
    18721 aattttgctt cagaatggtg cggtgcacct cgcaaaaaca aacaataaaa catcaatatc
    18781 tttacattta gccaaactaa atgttaccta acaggcacaa cactgcacaa taaagttgca
    18841 gacgataaca acacaaacac tcacaacggg tatccatgcg ttcttaacgc agaagataaa
    18901 ggtgttggat atgcaattac gtaaacctgc cacagcaatc ctcgccctgg cgctttccgc
    18961 aggactggca caggcagatg acgccgcccc ggcagcaggc agtacgctgg acaaaatcgc
    19021 caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca gtacctttct cttattacga
    19081 caatcagcaa aaagtggtgg gttactcgca ggattactcc aacgccattg ttgaagcagt
    19141 gaaaaagaaa ctcaacaaac cggacttgca ggtaaaactg attccgatta cctcacaaaa
    19201 ccgtattcca ctgctgcaaa acggcacttt cgattttgaa tgtggttcta ccaccaacaa
    19261 cgtcgaacgc caaaaacagg cggctttctc tgacactatt ttcgtggtcg gtacgcgcct
    19321 gttgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaaggca aagccgtagt
    19381 cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa ctgaatgaag agcaaaaaat
    19441 gaatatgcgc atcatcagcg ccaaagatca cggtgactct ttccgcaccc tggaaagcgg
    19501 tcgtgccgtt gcctttatga tggatgacgc tctgctggcc ggtgaacgtg cgaaagcgaa
    19561 gaaaccagac aactgggata tcgtcggcaa gccgcagtct caggaagcct acggttgcat
    19621 gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gacactatcg ctcaggtgca
    19681 gacctccggt gaagcggaaa aatggtttga taaatggttc aaaaatccaa ttccgccgaa
    19741 aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa
    19801 tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc tctcgattgt
    19861 tacgtagcac ggacagacta tacgcctgat ggtcgttccc catcgggcct gaaaaccgca
    19921 atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct tttttctgga
    19981 gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc ccgttcggca
    20041 ataccaccta tcttggttgg gtctggagtg gttttcaggt gacgatcgct ttgtcgatct
    20101 gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt accgttccaa
    20161 accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac gtgccgttga
    20221 ttgtgcaatt ctttacctgg tatctggtga tcccggagct gctgccggag aaaatcggca
    20281 tgtggtttaa ggccgagctg gatcccaata ttcagttttt cctttcatcc atgctctgcc
    20341 tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgctatt cagtcgctac
    20401 cgcgtgggca gaagaatgcc gcgctggcga tgggcctgac gctaccgcag acttatcgat
    20461 acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacttcc gagatgatga
    20521 acctggtgaa aaactccgcc atcgcctcga ccatcggtct ggtggatatg gcggcgcagg
    20581 cgggtaaatt gctcgactac tcggctcacg cctgggagtc gtttaccgcc atcacgcttg
    20641 cgtatgtgtt gattaacgct tttatcatgc tggtgatgac gctggttgaa cgtaaagttc
    20701 gcctgcccgg caacatgggg ggcaaataat gtacgagttt gactggagtt ccattgttcc
    20761 ttccctgcca tatctgctcg acgggctggt gatcaccctg aaaatcaccg tcacggcagt
    20821 cgtgattggt attttgtggg gtaccatgct ggcggtgatg cgcttgtcca gcttcgcgcc
    20881 tgtttcgtgg tttgccaaag cctacgttaa cgtattccgc tcgattcctt tagtcatggt
    20941 tttgctgtgg ttttacttga ttgtgccggg atttctgcaa aatgtgctgg gattatcgcc
    21001 aaaaaatgat attcgtttaa tttcggcgat ggtggcgttt tcgatgtttg aagcggccta
    21061 ctattcagag attatccgtg ccggtattca aagtatttcc cgtggtcagt cgagcgccgc
    21121 gctggcgttg gggatgactc actggcagtc gatgaaactg attattctgc cgcaggcttt
    21181 ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta ctgttccagg atacctcgct
    21241 ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc tcaaccattg gtgagcgtga
    21301 tggtacgcag gtcgagatga tcctgtttgc cggatttgtt tatttcgtta ttagccttag
    21361 cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac cctgaaaaat
    21421 gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga agtgaaaaaa
    21481 ggcgaagtgg tggtggtttg cggcccgtct ggttccggca aatcaacgct gattaaaacc
    21541 gtcaacggcc tcgaaccagt gcagcaaggt gaaatcaccg tcgatggtat cgtggttaac
    21601 gacaagaaaa ccgatctggc aaagctgcgc tcccgcgtcg ggatggtatt ccagcatttc
    21661 gagctgttcc ctcatctgtc gattatcgaa aacctgaccc tggcgcaggt gaaagtgctt
    21721 aaacgcgata aagcgcccgc tcgtgaaaaa gcattgaaat tgctggagcg cgtgggactt
    21781 tccgcacatg ccaataaatt tccggcgcag ctttccggcg gtcagcagca gcgcgtggca
    21841 atcgctcgcg cattgtgtat ggctcctatt gcgatgctgt tcgacgaacc gacatcggcg
    21901 ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tggaactggc gaacgaagga
    21961 atgaccatga tggtggtgac ccacgaaatg ggctttgccc gcaaagtggc gaatcgggtg
    22021 atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt cttcgatgat
    22081 ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc tgcattaatc actctggcgc
    22141 acatacccaa ttgcgcgcca tctcacgctt tatcgtttcg cccctcgtat ctgttacatc
    22201 ctggcgttac ccttgtcgca aagaagcaca caacaaggag caacaatggc actgccaatt
    22261 ctgttagatt gcgacccagg tcatgatgac gctatcgcaa tagttctcgc actcgcctca
    22321 ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc agaaaaaacc
    22381 ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc ggtggcaggc
    22441 ggcgcggtaa aaccgttaat gcgtgagttg attatcgcgg acaatgtgca cggcgaaagc
    22501 ggtctcgacg gcccggcatt accggaaccg acgttcgcac cgcaaaactg tacggcggtg
    22561 gagctgatgg cgaaaacgct gcgcgaaagt gcggaacctg tcacgattgt ttctaccgga
    22621 ccgcaaacta acgttgcctt gctgctcaat agccatccgg aactgcatag caaaattgcc
    22681 cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc ggctgaattt
    22741 aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcagggat cccggtggtg
    22801 atggccggtc tggatgttac tcataaagcg caaatccacg ttgaagacac cgagcgtttc
    22861 cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt cttcctcgaa
    22921 tatcataaag acgaaaaatg gggctttgtc ggcgcaccgc tgcatgaccc atgcaccatc
    22981 gcctggctgt tgaaacccga gttgtttacc actgttgagc gctgggttgg cgtggaaaca
    23041 caggggaaat atacccaggg tatgacggtt gttgattatt attatctgac tggcaataaa
    23101 ccgaatgcca ctgtaattgt cgatgttgat cgtcagggct ttgttgattt actggctgat
    23161 cgtctgaaat tttacgctta acactcgtca ttgattaaga tcaccacgcg ttacctgggt
    23221 ggtcttaatc tatttatctc tttttctttc aagatataaa tgcaatggat aatgcagaac
    23281 tcgccatagg tatcgatctc ggcactacca atagtttaat tgccgtctgg aaagacggtg
    23341 ccgcgcaatt aattccaaat aagttcggtg aatatttaac accatccata attagcatgg
    23401 atgaaaataa tcatatttta gtcggaaaac cggctgtatc acggcgtact tcgcatccgg
    23461 ataaaacggc agcgttattt aaacgtgcaa tgggcagtaa taccaactgg cggttaggca
    23521 gcgacacatt taacgcgcca gaactgtcct ctttggtatt acgctcatta aaagaagatg
    23581 ccgaagaatt tctgcaacgt ccgattaaag atgtggtgat ctccgttccg gcttatttca
    23641 gcgatgaaca acgcaagcat acccgtttag cagcggactt agccgggtta aatgcggtac
    23701 gcttaattaa tgaacccaca gcagctgcga tggcgtatgg cctgcatacc caacaaaata
    23761 cccgttcgct ggtgtttgat ctcggtggcg gcacgtttga cgttacggtg cttgagtacg
    23821 ccacgccggt gattgaagtt cacgcctccg ctggcgacaa cttcctcggt ggcgaagatt
    23881 ttacccatat gttggtcgat gacgttttaa agcgcgcgga tgtcacaagg accacgctta
    23941 acgagagtga actggcagcc ttgtacgcct gtgtggaagc ggcaaaatgt agcaatcaat
    24001 cgccaatgca cattcgctgg tagtaccagg atgaaacgcg ggaatgcgaa ttttacgaga
    24061 acgaactgga agatttgtgg ttgccgctgc tcaatcgctt gcgagtgccg attgaacagg
    24121 cgttgcgcga tgcgcgtctg aagccgagtc aaatcgacag tctggtgctg gttggcggcg
    24181 cgtcacaaat gccgctggtg cagcgaatcg ccgtgcgtct gtttggcaaa ttaccgtatc
    24241 aaagttacga tccgagcacc attgtcgcgc tgggcgcagc aatccaggcc gcctgccgct
    24301 tacgcagtga agatattgaa gaagtgatcc tcactgatat ttgcccttac tcgttgggcg
    24361 ttgaggttaa ccgccagggc gtttccggca ttttctcgcc gattattgaa cgaaacacca
    24421 ctgtgcccgt gtcgcgtgta gaaacttatt caaccatgca cccggaacag gattcaatta
    24481 cggttaacgt ctatcaggga gaaaaccaca aagttaaaaa caacattctg gtggaatcct
    24541 tcgatgtgcc gttgaagaaa accggtgctt atcagtcgat tgatattcgc tttagttatg
    24601 atatcaacgg gttgcttgaa gttgacgtgc ttctggaaga cggcagcgtt aagtccagag
    24661 tgattaacca cagcccggta acattgagcg cgcagcagat tgaagagagt cggacgcggt
    24721 tatccgcatt gaaaatttat ccgcgcgata tgctcatcaa tcgcaccttt aaagccaaac
    24781 tggaagagtt gtgggcgcgg gcgctgggtg acgagcgaga agagattggc cgggtgatca
    24841 ccgattttga tgcggcgctg cagtcaaacg atatggcccg cgtcgatgag gtcaggcggc
    24901 gggcgagcgt ttatttagcc attgagacct cataatcacg gctgccggat gcggcgtaaa
    24961 tgctgcatcc ggcatcggcg caaaaaacac atcgtcaact aaacaacatc gcgattttgc
    25021 cgagagcata acctataacc agcaccgtta aactgatttt gataatgctg gtaatgctct
    25081 gtggtttttc accctcatca tttttttctt catggttttc aggtgttggc tcatcgacat
    25141 cttcatcctc cggctcgagt tttactttcg tcaacagcat ccgggtgata atggcctcta
    25201 atggatgagc atctccgcct tccagccttg tattaagacg ggccagcatc tcttgtttcg
    25261 gaaacgcttc aagtactacc tgttccgcta cccgatacaa atccagtaac ggtgaatctt
    25321 cttctgtctc gctaccgact ctcgatttcc accacgccca tgctttgcgt ttgaagaata
    25381 acgactcaat aaacgattga cctaacggcg ataattttcc gctatgtaaa atttccgccc
    25441 ataacagccg cgtccgggcg ctctcaccag gaacatattt cagcggttga ccttgcgcat
    25501 ctaccatgct ccgcgcttcg ataaccatca ctaataacgc gaaataatcc gggcaatgac
    25561 gcttgctcca ctgcaacaac aaattatcag cttgtgtaga tgggtaactt tcccagtacg
    25621 cgccgagatc agcaagcagg ctttccccct cgccgcagta caaacgttgt gcacactggt
    25681 aataccgggc atcttcgctt tctggttctt ccgcttgcca ctgtttggct acaggaatga
    25741 gttccaccat acctgtttgc accgagctgt accagcgcaa cagtttgcga tgtaactttg
    25801 catcatcagg aatgaaccac ggtccttcca tcgccaaaaa cgcagtgaca tagtcaggat
    25861 ggtaacggca gcaacgttcc agcgcgaagt aatattcaac agtctggttc tgtaacgcga
    25921 cgggatattc cagaaggcta aggaaatcga aacaatcacc agcctgaatg gcctcaagaa
    25981 aagcctccct ttcttcctca tccattcctt cgctgtcatt aaacgaatgc cagttcaaac
    26041 gctctgccaa caaagaagca caatttaatg acagatatcg cgcttctata gcgattgcac
    26101 acagcggcca gcgtaattga tcgacatcct ccatgttcca ggtatttaat tgctgaataa
    26161 atttctgcca ggcggagggc tgaaaacgat cactttctga atccagtaat gtcctgaatg
    26221 cacgtagtat ttcatgttca gcggtggcat ctttttcttc atcatctgct tcctcgacag
    26281 gatttaccgc cagtcgcagc gcctcttcat aggcctggcg taatgcttta aacccctgcg
    26341 gatcggtttc gggatgacac aacggtaagc gagcaagata agcctggcgg ataatgtcta
    26401 tttgcgtcgt gctttcaatt tcgaggattt gccaacatgt tttcataggt cgctcacatc
    26461 cttaagcgtt accgggcatt caggctggac agttaatggc tgccagggaa gcgtggcata
    26521 gaaacggtcg cttcccgctg ccagctcttc gaacatccga ctcccggcgc tatcgtattt
    26581 gtacagggtt agcgcctcgc gtagcgaagc gttgtcttcg cactgagaag attgccagta
    26641 gagacgccct agtgaatagg cagaaaaata gtgcatccag ctgtgatagt aatggtgagc
    26701 tcgtgcatag acgcgcgatt gcagccataa actctcttct tccgtcagcc agttgttgag
    26761 cacaccggcc cgtgacagaa accccattcg aacataatcc caggctttga ttcccccttc
    26821 tccacagcac atagcggttt gggcaacgaa ttgggtataa atcaaccctc tctccgagcc
    26881 accagcaatt aatgctttcc actcttcagg ggaagagcgt tgccatgtca gatataagcc
    26941 agctaaatca ttggcatgac catcatcagt catctgataa atagtctgaa ttaaatcatc
    27001 acgattattt attccccagg atgatgagag atcgaattct tttatcccgg tatacatttt
    27061 tggcgagtca tgcgcgggtt tgcaactctg attaagtatt tccatgggcg cagaaagcgc
    27121 aaaaagaaga tattgttctt ccttattcat tttttaataa tcccttaagt gcattaactg
    27181 actacttttt tattattgat gagtataaac ttgacgcttt caaaataaaa agaaaatcga
    27241 agcattcaca catgaataaa aaattaatgt atatattcgc aatttttata gttgcagcaa
    27301 ttacctgtat tagccaaccc aagaaaacga tgttgcgtga taaagccatg gtgaattatg
    27361 cctttgatta tttaagatca ccaggcagtc taccgttaac cacggcagcc acggagcttt
    27421 ccgcgattca tggtcactca acatcgcaat atcgccttgg cgaattttat cttcatggta
    27481 gcgacggtaa accactggat tatacacagg cgagatactg gtatgagcaa tcagcagaac
    27541 aggaaaatcc gcgcgcacaa agcaaactgg ggtggatcta cctgaaaggc ctgggagtca
    27601 aacccgatac ccgtaaagca attctctggt ataaggaagc agctgaacaa gggtatgctc
    27661 atgctcaata tactttgggt ttgatctaca gaaatggcac tggtattaat gttaaccatt
    27721 atgaatctca aaaatggtta aaactggccg ccaaacaaca ttacaaaaat gcggaaagat
    27781 tacttgccgg acttcccgca cattaacgtc gactgtcacc cgaagagatg gagtattttc
    27841 cctatcaaac cggcaaagat aaaaatgtag aaaataattt tgatgacgcc cagacagccc
    27901 agcccgccat catcaacggg cattggctgc ggttgcggct ccggctcgac ggtttcctga
    27961 ccggtaaaat tcgccgcctg aattaataat cgacgaataa ttaatgcttc cagtggacaa
    28021 cgatcttctg catcaaggcg gctcacaatc ggcattaatg attcttctgt cggttggctg
    28081 gcctgcacta attgtttggc aactaaatat aatggccata agggatgttc tgtatcacgc
    28141 gtgagtgaga tccgcggctt acgtcctttg tgcaaaacca tctcgaccag tgaacgcccc
    28201 agcgcagaga gtttttcatc gctgagtatt tcgtataaat gaaaacgcgt acgggcgctg
    28261 tcgccgggga cataaagtag cggttttccc tgatcgttaa ccagatcacg cgcttcaatc
    28321 atcatcacca ctaatgggta ataatagacc cgatgctgac ggcaccattg caacattaaa
    28381 gcatccgcct gggtggaggg atactcgcgc cagtagtaac aaagttctgc cagcaggctt
    28441 tcgccttctc cacaatagac gcgttgtgcg tattcataat aacgcgcatc ttcattatca
    28501 ggttcagctt cccgccacga ccgggcgtaa tccaggagtt cggggattcc caggcggctt
    28561 gcgctatacc agcggagtaa atttctgtgc agttttgcat catcggggat aatcactgcg
    28621 cgatgttgcg ctaaatacaa tgtcagccat tctggatgcg atgaccaaat ccgagcgaac
    28681 atttggtaaa aatcgatggt gtcattttgc acggcaactg gcagatgcag aatggtctgg
    28741 aagttaaaaa cattcccctt cgcaatggca tataaaaagg attccagttc ttcttcgtct
    28801 atttccccga cgttttcctc ctgcaaccac cgcaatcttt ctgctaataa caccacgcac
    28861 tcgaaggata aatgggcagt gttcatggcg attgtgcaca gcgaccagcg taattcatca
    28921 atctcttcca tcgagcaata atttaattgc tgaatgaatc gctgccaggc ggaggacaga
    28981 aaacgttcgc tatcagaggc aagtaacgcg cgaaaggcga gcagaatttc atgttctgct
    29041 acctcatatt cttctggttg ccaaacagat ttagcaggcg actgcgcaat ccgtagcgcc
    29101 tcctcatacg cctgacgaag ttgtttaaaa ccctgcggat cggtttctgg atgaaaggac
    29161 ggtaacagcg ccagataagc gcggcggata atatcgacgt cagtcgtttc ctctatatcg
    29221 aggatcttcc agcaattttt cataagtcgc tcatatcctt aagcgtttcc gggcatgtgg
    29281 ggtagtcagc cagtggttgc caggacaagg tagggtaata cgcatcatca ctggcaaata
    29341 attgataaaa catatcattg ccggaatcgt cgtattcttt acgcgctaag aattcgcgta
    29401 ataaaggcag atgatcatct tcgggagact gccaatattg ccgaccaaag gtataaccgg
    29461 caaagtattg ccgccagtta ctataataac gtagagcccg tagatgaata cgcgactgga
    29521 tccatagact ttcttcttca ctcaaccagt tattaagtac gcccattcga cttaaaaaac
    29581 ccatccggac ataatcccat gctttaattc ctccctcacc acagcattcg gctgtgctgg
    29641 cgacaaattg cgcataagcc tgaccttgct catttaattc agcaagatag tcacgccatt
    29701 cacatggaga ataacgaaac cagcggtgat aaaaccctgc caaccgagcg gcatgaccgt
    29761 tctcgatcat tcggtgaatt atgcgcagca ggtcatcacg gtcatcaatt ccccatgagg
    29821 aagaaaggtc aaaaaagcat gtacctaaat acattttggg ggatgaatgc gaaggtaaac
    29881 agcattcatt gcgtatttcc atcggagtgg aaagtgcaaa caatattttt tgcgacccca
    29941 tgtccatata atttatcctt aaacattcat ttaatgaatg taaactacat ttcgaggctc
    30001 agttatttat acttttctga cgcaacataa tacgcataaa ataaggctta cgcaaaaatg
    30061 gaatacataa aaatataata tattttatct acaaccataa ggaaatgata atgattttta
    30121 cgtcaagttg ctgcgataat ttatcaatag atgagattat cgaacgtgct gaaaaaggcg
    30181 attgcgaggc tcaatatatt gttgggtttt attataatcg cgatagcgca attgactctc
    30241 cggacgacga aaaggccttt tactggctga agctggccgc tgagcaaggt cattgtgaag
    30301 cacagtattc cttagggcaa aagtataccg aggataaaag ccgtcataaa gataatgagc
    30361 aagccatctt ctggctgaaa aaagctgccc tacaaggcca tactttcgct tccaacgccc
    30421 ttggctggat actggatcgt ggagaagacc ccaactataa agaagcggtt acctggtatc
    30481 agatagccgc ggagagcgga atgtcttatg cgcaaaataa tcttggatgg atgtacagaa
    30541 atggcaacgg agtcgcacaa gactatacgc tggcattttt ctggtacaaa caagctgcat
    30601 tacaaggcca tagttacgca caagacaatc tggccgatct ttataaagac ggagaaggcg
    30661 ttgctcaaaa caagacactc gccgcattct ggtatttgaa aagcgcacag cagggtaatc
    30721 ggcacgccca gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg gaccaggact
    30781 ataagcaagc gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc ggcgcttacg
    30841 tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat caggctgcct
    30901 ttgaatggtt tacgaaagcc gctgaatgca atgacgccac tgcctggtat aacctggcca
    30961 ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc gacctgtatc
    31021 gtaaagttca gtcatccgga accagagatg tcagtcagga aattcgtgag actgaagctt
    31081 tactgtagaa ataaaaacaa gggccgggga atttcattct gcccatattc tctttattgc
    31141 tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc ataaccacat
    31201 ttcgggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg
    31261 cagaccagat ttcccagccc gaccacttct ccgctgtgat aaaccccatg atgattgagg
    31321 tcctggaaaa tttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc cagctcctgc
    31381 cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt caggctctct
    31441 tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag ctcatcgatc
    31501 tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg
    31561 tcgatatcac gttcgccatt gcgcaggcgt tcgttcagtg acgcgaccag ttcacggtaa
    31621 tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga
    31681 cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtacgaata
    31741 atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg acgtttgggc
    31801 tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta ttgaaaacag
    31861 gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc
    31921 ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt
    31981 actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac gtacgtaact
    32041 acaccatcgg tgacgtgatc gcccgttacc agcgcatgct gggcaaaaac gttctgcagc
    32101 cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg
    32161 ctccggcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg
    32221 gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac taccgttggg
    32281 aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataaaaag acttctgcgg
    32341 tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct
    32401 gctggcgctg cgataccaaa gttgaacgta aagagatccc gcagtggttt atcaaaatca
    32461 ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg
    32521 ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggagatc accttcaacg
    32581 ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc tttatgggtt
    32641 gtacctacct ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg gaaaataatc
    32701 ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg
    32761 cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca ttaacgggcg
    32821 aagaaattcc cgtttgggca gcaaacttcg tattgatgga gtacggcacg ggcgcagtta
    32881 tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca
    32941 tcaaaccggt tatcctggcg gctgacggct ctgagccaga tctttctcag caagccctga
    33001 ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat gaagcggcct
    33061 tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc
    33121 gcctgcgcga ctggggtgtt tctcgccagc gttactgggg cgcgccgatt ccgatggtga
    33181 cgctggaaga cggtaccgta atgccaaccc cggacgacca gctgccggtg atcctgccgg
    33241 aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa
    33301 ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc tttatggagt
    33361 cctcctggta ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg ctggattccg
    33421 aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa cacgccatta
    33481 tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact
    33541 ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact
    33601 atgttggcga aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg
    33661 acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg
    33721 gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac
    33781 gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc
    33841 tcgaatggca ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac
    33901 tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgttgat gcgctgactg
    33961 aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagtg accgatgata
    34021 tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac
    34081 tggcgaaagc accgaccgat ggcgagcagg atcgcgctct gatgcaggaa gcactgcagg
    34141 ccgttgtccg tatgctcaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac
    34201 tcaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg
    34261 tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc aaaatcaccg
    34321 ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag
    34381 caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt aaactcctca
    34441 atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacac tgttgttatc
    34501 tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca cgcaggttcc
    34561 ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc
    34621 ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa
    34681 ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt
    34741 ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat
    34801 ccccggccgt gatatctacc cgattagcgc caaagtcttc cgttcattct tcgataaccc
    34861 gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg
    34921 tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata ttcgttccga
    34981 cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccat
    35041 gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg
    35101 ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac
    35161 gctgttcgcc aggtagctgc ggcacaagga ttcgaagaac accacacttt ttccattgat
    35221 cccaacactg actggaatgc gatcttttcg ttatgccagg ctatgagtct gtttgccagt
    35281 cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat caatgaacaa
    35341 cttctcacac tcaccggact tctgcatgac gacctgctat tgatcgtccg cggtaataaa
    35401 ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag
    35461 gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt gcgcgcaaaa
    35521 cagctcaact tagaactgga tgacgcggcg aatcaggtgc tctgctactg ttatgaaggt
    35581 aacctgctgg cgctggctca ggcactggag cgtttatcgc tgctctggcc agacggcaaa
    35641 ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat
    35701 tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct tcagcaactg
    35761 cgtctggaag gcagcgagcc ggttattttg ttgcgcacat tacaacgtga actgttgtta
    35821 ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat
    35881 cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt aagtcagccg
    35941 cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac
    36001 ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca taaacccctg
    36061 gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt
    36121 tgatccggtg cactatggtc atctaaaacc cgtggaaacg ctggcgaatt tgattggtct
    36181 gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa
    36241 cagcgtgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct
    36301 tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg
    36361 gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg attcattgct
    36421 gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg
    36481 tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga
    36541 tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc
    36601 tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga
    36661 atcgtgtgag gatttattgc cggaaccggt attgacttac attaaccaac aaggcttgta
    36721 tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggcgaa
    36781 acacaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct gaccgcacgc
    36841 ggtattgagc aagcgcaaaa tctgcataca ctgctagatg atgtttcctt tgacctggtt
    36901 ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttctcag tgaccgccag
    36961 ctccccgttc atatcatccc tgaactcaac gaaatgtttt ttggcgactg ggagatgcga
    37021 catcatcgcg acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg
    37081 cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaacgc
    37141 tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttaatcgt cagccatcag
    37201 ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc tatgtggcat
    37261 tttcgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta
    37321 cgcgtcctca atagccgtgc catcggggtc gagaacgcat gacttttctg tttttttacg
    37381 ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc agccagactt
    37441 tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc
    37501 gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg
    37561 cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa
    37621 cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca
    37681 ggacatcatc gccttagacg ttcaaggcaa atccagcatc accgactgca tgatcatctg
    37741 tacgggtacg tccagccgtc atgttatgtc cattgctgac cacgttgtgc aggagtctcg
    37801 cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt
    37861 ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact
    37921 ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc
    37981 ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt
    38041 cgagctgatt gaaattccgg ccggcaaacg cggcaagaat gcggacatca agcgcatact
    38101 cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga
    38161 tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct
    38221 ggatggtcgc gacgtaagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa
    38281 agcggcggct gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc cgctggttcg
    38341 cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca
    38401 ccgtgagtga taagggagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt
    38461 tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg
    38521 gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt
    38581 taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag
    38641 ccgcggcatt atctacgacc gtaacggtat ccctctggcc ctcaaccgca ctatctacca
    38701 gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg ctttgcgcag
    38761 cgtggtagat ctgaccgatg acgatattgc tgcattccga aaagagcgcg cacgttcaca
    38821 ccgtttcacc tctattccgg tgaaaactaa cctaaccgaa gtacaagtag ctcgctttgc
    38881 cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc gttactatcc
    38941 ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt
    39001 cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg atattggtaa
    39061 gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt
    39121 tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaagccgg
    39181 acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc
    39241 gggtagccgc gcagctgtgg ttgttaccga accgcgtaca ggtggggtgc tggcgctggt
    39301 ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc
    39361 cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc
    39421 tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttaagcgccg gggtgatcac
    39481 gcgcaatacg acgctgtttg acccaggctg gtggcaacta ccagggtcgg aaaaacgtta
    39541 tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc
    39601 tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg
    39661 gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg
    39721 caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt atcagggtga
    39781 caccattccg gttggtatcg gccagggtta ctggaccgcg accccaatcc agatgagtaa
    39841 ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc tgatgagcac
    39901 cgccgaagac ggcaaacagg tgccatgggt acaaccgtat gaaccgcccg tcggcgatat
    39961 tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa
    40021 tggtacggcg aataaatact ttgccagcgc accgtacaaa attgcggcga aatctggtac
    40081 cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg
    40141 tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt
    40201 cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga tgcgccagat
    40261 cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt
    40321 tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat
    40381 aaagtccatc tcgatcccac aatgctgctg attttactgg cattgctggt ttacagcgcc
    40441 ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa aatcggccaa
    40501 atcgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa
    40561 ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc
    40621 ggagccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg ttttcagccg
    40681 tcggaaattg ccaaaatagc cgtaccactg atggttgcgc gctttatcaa ccgcgacgtt
    40741 tgcccgccat cgttgaagaa tactggcatc gcgctggtgc tgatatttat gcccacgctg
    40801 ctggtggctg cacagcctga cctgggaaca tcaatcctcg ttgcgctttc cggtctgttt
    40861 gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtagcg
    40921 gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg
    40981 atgctcctgg acccggaatc agacccactc ggcgcgggct atcacattat tcagtctaaa
    41041 attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac tcagtcacag
    41101 cttgaatttc tccccgaacg ccatactgac tttatcttcg cggtactggc ggaagagctg
    41161 ggattagtgg gcattctgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg
    41221 tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg cttaatgctg
    41281 atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta
    41341 ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct gatggctggg
    41401 ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag
    41461 gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg
    41521 tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg
    41581 ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg cgacggcaaa
    41641 tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag
    41701 ccaggcggga ctggcggcaa tctatgatgc cgaaccaggc agtaacctga cggcctctgg
    41761 cgaagctttc gatccgacaa agctgacggc ggcccatcca acgcttccga tccccagcta
    41821 cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg
    41881 cccttacggc aacgaccgcg ttatttcgct ttctcgcgcg gcagctgacc gtcttaacac
    41941 gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc
    42001 tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc ctgcacctcc
    42061 cgatttaagc ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg acattcttcc
    42121 ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagtagcgg
    42181 tttcctcggc gctccaacga ccttagcgcc tagtgtactg gaaggcagcg aaccgacgcc
    42241 tgcgccacag cccgttgtta cagcttcgtc gacaacgcct gcaacctcgc ctgcaatggt
    42301 gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt
    42361 aagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc
    42421 cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat tcgccagcaa
    42481 agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat
    42541 tactaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta
    42601 acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt
    42661 ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat
    42721 catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc
    42781 cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta
    42841 catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg
    42901 cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc
    42961 cggtaaattt aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa
    43021 cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc
    43081 tcagctgatc cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga
    43141 ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgcact
    43201 gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag
    43261 ctccgcgcga gatatggcgc tgatcggcca ggcattgatc cgtgacgtac cgaatgaata
    43321 ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg
    43381 cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc
    43441 aggttacaat cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat
    43501 gggtggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt
    43561 ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt
    43621 ttggtttagt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat
    43681 tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca
    43741 tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac
    43801 gatcgagcaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa
    43861 aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg
    43921 aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaaactcccg ccttctggcg
    43981 ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact taacgaactg
    44041 cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc tgagctggtt
    44101 gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc aacggtaaaa
    44161 ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac tcatatcgag
    44221 caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat ggttctgtaa
    44281 ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc tgtgatatac
    44341 ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg ataaaattct
    44401 tgtccgccag ctcggtcttc agccttacga gccaatctcc cgggctatgc atgaattcac
    44461 cgatacccgc gataatagta cccttgatga aatctggctg gtcgagcact atccggtatt
    44521 cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata ttccggtgat
    44581 ccagagcgat cgcggtgggc aggtgactta tcacgggccg ggacaacagg tgatgtatgt
    44641 gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct tgcttgagca
    44701 aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg ctgacgcgcc
    44761 tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgaattc gacgcggttg
    44821 ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt tacgtattaa
    44881 tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac ccgaagcgac
    44941 gactaataat attgctccac gtttactgga aaatatttta gcgctactaa acaatccgga
    45001 cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac tgggtcatta
    45061 ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc tttatcactt
    45121 gcagaaatgc ggtgccacgc cttgctccgt aactcacccc attgattaac ctattgaaat
    45181 agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta ttgtaatttt
    45241 ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat
    45301 tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta caaccttacg
    45361 aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata aagggatcgt
    45421 taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact
    45481 gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
    45541 atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct
    45601 ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca ctccctcggt
    45661 cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc cgcagctttt
    45721 actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct
    45781 catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga
    45841 caatatggtg ttaatttgcc gcaagggaaa tccactactc tctttagaag atgacagaga
    45901 gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt tcagcggtct
    45961 gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca gctacaacat
    46021 tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt
    46081 ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga
    46141 ggagcaaata gatttctcta tccactacaa caaattcatc ctgcgtgatc ccatcctgca
    46201 cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca
    46261 gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg
    46321 ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa
    46381 ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg
    46441 ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg
    46501 cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat
    46561 ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc
    46621 gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct
    46681 tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc cgttgtccgt
    46741 tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca gtgaaactgg
    46801 cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg
    46861 acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt cgggaaaaaa
    46921 gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg gatcgtgctc
    46981 tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa aacgtaccgc
    47041 gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag ctgctggaac
    47101 gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg gggctgggtg
    47161 aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt
    47221 taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta
    47281 gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg
    47341 catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa ggaatggaag
    47401 ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttgaagcg
    47461 ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg
    47521 agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc
    47581 attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa
    47641 cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact
    47701 aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc
    47761 agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc
    47821 aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag
    47881 atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt
    47941 gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc
    48001 ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca
    48061 cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac ccaggcggca
    48121 ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc tggaaagcgt
    48181 aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa cggagctatt
    48241 tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata gagatgcagt
    48301 ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt ccatgcgcgc
    48361 cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg tttactttct
    48421 cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac cgatagatct
    48481 gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga tacgtcgttt
    48541 tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca cacagatgta
    48601 acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg taaaattcgc
    48661 caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
    48721 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tagcgagatg
    48781 gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga cgctggcagc
    48841 aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa
    48901 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
    48961 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcatt
    49021 actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg cattctggct
    49081 gttttcggcc tcaaccgtac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
    49141 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
    49201 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
    49261 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
    49321 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
    49381 tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg tggtacgctt
    49441 tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat gttgcttatt
    49501 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
    49561 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
    49621 cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
    49681 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
    49741 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
    49801 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgacag
    49861 gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
    49921 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
    49981 ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag caaaaacttt
    50041 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt tactcacgtt
    50101 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
    50161 acataacctt aatagaccca acataaagaa taatctgaat agctgctcta tttatttccg
    50221 cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt ttctgcgatg
    50281 ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat caaggaaaaa
    50341 ttattttatt tttttaacga gatacaacaa tcatcttaac gaagtatata atatatatcg
    50401 tcaacccatg ctcatattag attatttttt gagcgcacag ccgcagctaa caccattgca
    50461 attaacaaat ttgcatcaat ccaccatcaa tttacacaca ttattatgtg ataattgcca
    50521 accgctaaat atgcgttttg ttatctatgt ataaaaacag caacttcaat gtcttaatgg
    50581 cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat tttttcctta
    50641 agctttagga agtttttatt atttactttg ggcctggaga caggaaaaat tatgctgaca
    50701 ttcattgaac tccttattgg ggttgtggtt attgtgggtg tggctcgcta catcattaaa
    50761 gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat tatcagtgcc
    50821 attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag cgccacggat
    50881 atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg catgatgatt
    50941 atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga tatggtggtc
    51001 aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat gattgccgcc
    51061 tattttgttg cttgtctgat gtcactggcc gtctcttccg caaccggtct gggtgttttg
    51121 ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagtcgtgg cgcagcagct
    51181 gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga tgtggtgctg
    51241 gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac gctgcctatc
    51301 tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg ttatctggat
    51361 aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac cactgccccc
    51421 gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt tgacggtaaa
    51481 tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat tgcctccatt
    51541 ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga agtggcttat
    51601 cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc tggggtattc
    51661 gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc tacctcgttt
    51721 ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct ggcggcagtc
    51781 acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc gaaactggcg
    51841 cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc gtcaaacctg
    51901 ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat ggcgaaaatc
    51961 tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct agtgattgtt
    52021 atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg caagtaaata
    52081 gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg tatatgcgtt
    52141 gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg ctggcgcaat
    52201 attcaagata acgcctggca gtggtgcggc tgatggtcag tgcctgcgcc accgtttctg
    52261 ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc gtcaaggggt
    52321 caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta aacatctcat
    52381 caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc tggcggaaac
    52441 gggttagcgt ttgccccagt gtattgattc acttgaagta cgaaaaaacc gggagaacat
    52501 tggattattc gggatctgat gggattagat ttggtggggc ttgcaagcct gtagtacaaa
    52561 ttttaggcgt taaccaatga aacgcgaaag atagtaaaaa attgcttttg tttcattgaa
    52621 aaaacgaaaa acaaaaacac tataaatcat ttcaatcata gcttcaaaaa acgttcaaaa
    52681 ccgataacaa ccaggctgtc accaaatgac tcatatcaca aatcagctca tgccgtttag
    52741 gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt agctggctca tggttgttat
    52801 acggcttttt tcgcatcttg tggttccaac aaaggagcta caggctcttc ctgtttatga
    52861 acaccataca ttgacaaaat ctcttggcga gtttctggag gtaatttatg cagcgccaaa
    52921 gctgtttgta gtgtttggcg atcaataccc agcgttgcca atagggtgtc aacggagatg
    52981 gaggggcgtt cttgcattaa tccggaaagc ccatatttag ccatgtttag ctctatctct
    53041 cggacttcat tttctcctag tttattgatt aacagggaga acattttcaa tgtatcagca
    53101 ttcatttctt cgatagcaat tttttgcata gccggtattg ccgggatata ttcaacccca
    53161 ggcccgcgac gcccttcttg tgaacgcttc ggccatcctt ctttctttgc tcgtttatgc
    53221 accccctgta ttgtccctgg cattccatcc agttcagtaa tttgtttggg tgtcatccac
    53281 tggtttatct catttgcgct tttcataaac taagtttacc agatttatct atataaaaat
    53341 caatggatta caaaataaac cacattaact gatataaacc agttttgttt atttggtaat
    53401 gtataaaaca gataatcaag acgaattatt tgttttggta accctttatc atacgcaata
    53461 tggttggtgt gcgaaagatg agtaacgatt ggcatcgggc cgacgtgatt gcggccctca
    53521 aaaaaacagg aacccctctg gcaaaattgt ccagacgttc aggttattca accgggacgc
    53581 ttgcaaatgc attagtcagg ccatggccta aaggtgaacg cattattgct gacgccttaa
    53641 acatgaaacc tgaggaaatt tggcctagta gatatctaag ggatcacaat gagagagtgg
    53701 atatcagcaa ttgaatgcat gggtttatct ggtttacctg gttcattaat gggggttcac
    53761 aaaagagctc agagagaagg atggctcaag agaagaagag aaggaattag aggggttgct
    53821 tacgaatacc atatatcttc tcttcctaac attgctcgag ctgaagtttt gctccaacaa
    53881 ggaaaattta aaaccagtca gggatgtttt gaaatcgccc gccccacgct ggaagctcat
    53941 gattatgatc gtgaggcact atggagcaaa tgggataaag ccagcgattc ccagcgcagc
    54001 ctggctgaaa aatggttgcc ctcaattcag gccacagacg aaatgctgaa ccaggggatt
    54061 tcaacgaaaa cggcttttgc gaccgttgca gggcattacc aggtcagcgc atccactttg
    54121 cgggacaagt attaccaggt acagaagttt gcgaagcctg actgggcggc tgcacttgtt
    54181 gatggacgtg gagcatcccg tcgcaatgtt cacaaaagtg aatttgacga ggatgcctgg
    54241 cagtttctga ttgcagatta tctgcgaccg gaaaaacccg ccttccgcaa atgttatgag
    54301 cgtctggaac tggcagtccg cgagcatggc tggagtattc cctcccgtgc cacggccttt
    54361 cgccggattc agcaactgga cgaggcaatg gttgttgcct gtcgtgaagg tgaacatgca
    54421 ctgatgcatc tgataccggc acagcagcga actgtggaac acctggacgc catgcagtgg
    54481 atcaacggcg acggttatct gcataacgtc tttgtacgct ggtttaacgg tgatgtgatc
    54541 cgtccgaaaa catggttctg gcaggatgtg aaaacccgaa aaattctggg ctggcgctgc
    54601 gatgtgagcg agaacattga ttcaattcgc ctctcgttca tggatgttgt gactcgctac
    54661 agtatcccgg aggattttca catcaccatt gataacaccc gtggtgctgc gaataaatgg
    54721 ctgacgggag gcgcgcccaa tcgctaccgc tttaaggtaa aagaggacga tccaaaagga
    54781 ctgtttttac tgatgggtgc gaaaatgcac tggacaagcg ttgttgccgg taaaggctgg
    54841 ggccaggcaa aacctgttga acgtgctttc ggtgttggtg ggcttgagga atacgttgat
    54901 aagcatccgg cactggctgg cgcatatacg gggccaaatc cgcaggcaaa acctgataac
    54961 tatggcgacc acgctgttga tgcagagctg tttctgaaaa cctttgccga aggtgtggcg
    55021 atgttcaatg ccagaacagg ccgtgaaaca gaaatgtgcg ggggcaaact ctcgtttgat
    55081 gatgttttcg agcgtgaata cgccagaacg attgtgcgta agccaaccga agaacaaaaa
    55141 cggatgctgt tactgcctgc cgaggcggtg aacgtttcac gcaaaggcga gtttacgctt
    55201 aaagttggcg gctcccttaa aggcgcgaaa aacgtttatt acaacatggc attaatgaat
    55261 gccggagtga aaaaagttgt ggtcagattt gatccacagc agttacacag cacggtttat
    55321 tgag
//
LOCUS       DAECOD010000015        54486 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.015, whole genome shotgun sequence.
ACCESSION   DAECOD010000015 DAECOD010000000
VERSION     DAECOD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.015"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            24..656
                     /gene="comR"
                     /locus_tag="KKO15_000883"
     CDS             24..656
                     /gene="comR"
                     /locus_tag="KKO15_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBD6359775.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(718..1256)
                     /locus_tag="KKO15_000884"
                     /pseudo
     CDS             complement(718..1256)
                     /locus_tag="KKO15_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
     gene            complement(1483..2787)
                     /gene="ndh"
                     /locus_tag="KKO15_000885"
     CDS             complement(1483..2787)
                     /gene="ndh"
                     /locus_tag="KKO15_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBD6359776.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3195..3737)
                     /locus_tag="KKO15_000886"
     CDS             complement(3195..3737)
                     /locus_tag="KKO15_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD6359777.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3760..4785)
                     /gene="nagZ"
                     /locus_tag="KKO15_000887"
     CDS             complement(3760..4785)
                     /gene="nagZ"
                     /locus_tag="KKO15_000887"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBD6359778.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4796..5620)
                     /gene="thiK"
                     /locus_tag="KKO15_000888"
     CDS             complement(4796..5620)
                     /gene="thiK"
                     /locus_tag="KKO15_000888"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBD6359779.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5601..6242)
                     /gene="lpoB"
                     /locus_tag="KKO15_000889"
     CDS             complement(5601..6242)
                     /gene="lpoB"
                     /locus_tag="KKO15_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBD6359780.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6257..6634)
                     /locus_tag="KKO15_000890"
     CDS             complement(6257..6634)
                     /locus_tag="KKO15_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBD6359781.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6637..6996)
                     /gene="hinT"
                     /locus_tag="KKO15_000891"
     CDS             complement(6637..6996)
                     /gene="hinT"
                     /locus_tag="KKO15_000891"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBD6359782.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7335..9520
                     /gene="fhuE"
                     /locus_tag="KKO15_000892"
                     /pseudo
     CDS             7335..9520
                     /gene="fhuE"
                     /locus_tag="KKO15_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9580..11013)
                     /gene="ptsG"
                     /locus_tag="KKO15_000893"
     CDS             complement(9580..11013)
                     /gene="ptsG"
                     /locus_tag="KKO15_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBD6359783.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11308..12105)
                     /locus_tag="KKO15_000894"
     CDS             complement(11308..12105)
                     /locus_tag="KKO15_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6359784.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12116..13120)
                     /gene="holB"
                     /locus_tag="KKO15_000895"
     CDS             complement(12116..13120)
                     /gene="holB"
                     /locus_tag="KKO15_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBD6359785.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13117..13758)
                     /locus_tag="KKO15_000896"
     CDS             complement(13117..13758)
                     /locus_tag="KKO15_000896"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBD6359786.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13748..14770)
                     /gene="yceG"
                     /locus_tag="KKO15_000897"
     CDS             complement(13748..14770)
                     /gene="yceG"
                     /locus_tag="KKO15_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBD6359787.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14773..15582)
                     /gene="pabC"
                     /locus_tag="KKO15_000898"
     CDS             complement(14773..15582)
                     /gene="pabC"
                     /locus_tag="KKO15_000898"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBD6359788.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15702..16943)
                     /gene="fabF"
                     /locus_tag="KKO15_000899"
     CDS             complement(15702..16943)
                     /gene="fabF"
                     /locus_tag="KKO15_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBD6359789.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17030..17266)
                     /gene="acpP"
                     /locus_tag="KKO15_000900"
     CDS             complement(17030..17266)
                     /gene="acpP"
                     /locus_tag="KKO15_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD6359790.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17477..18211)
                     /gene="fabG"
                     /locus_tag="KKO15_000901"
     CDS             complement(17477..18211)
                     /gene="fabG"
                     /locus_tag="KKO15_000901"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBD6359791.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18224..19153)
                     /gene="fabD"
                     /locus_tag="KKO15_000902"
     CDS             complement(18224..19153)
                     /gene="fabD"
                     /locus_tag="KKO15_000902"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBD6359792.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19169..20122)
                     /gene="fabH"
                     /locus_tag="KKO15_000903"
     CDS             complement(19169..20122)
                     /gene="fabH"
                     /locus_tag="KKO15_000903"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBD6359793.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20190..21260)
                     /gene="plsX"
                     /locus_tag="KKO15_000904"
     CDS             complement(20190..21260)
                     /gene="plsX"
                     /locus_tag="KKO15_000904"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBD6359794.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21341..21514)
                     /gene="rpmF"
                     /locus_tag="KKO15_000905"
     CDS             complement(21341..21514)
                     /gene="rpmF"
                     /locus_tag="KKO15_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBD6359795.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21566..22087)
                     /gene="yceD"
                     /locus_tag="KKO15_000906"
     CDS             complement(21566..22087)
                     /gene="yceD"
                     /locus_tag="KKO15_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBD6359796.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22286..22870
                     /gene="yceF"
                     /locus_tag="KKO15_000907"
     CDS             22286..22870
                     /gene="yceF"
                     /locus_tag="KKO15_000907"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBD6359797.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22982..23941)
                     /gene="rluC"
                     /locus_tag="KKO15_000908"
     CDS             complement(22982..23941)
                     /gene="rluC"
                     /locus_tag="KKO15_000908"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBD6359798.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24514..27699
                     /gene="rne"
                     /locus_tag="KKO15_000909"
     CDS             24514..27699
                     /gene="rne"
                     /locus_tag="KKO15_000909"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBD6359799.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27896..28849)
                     /gene="flgL"
                     /locus_tag="KKO15_000910"
     CDS             complement(27896..28849)
                     /gene="flgL"
                     /locus_tag="KKO15_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBD6359800.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28861..30503)
                     /gene="flgK"
                     /locus_tag="KKO15_000911"
                     /pseudo
     CDS             complement(28861..30503)
                     /gene="flgK"
                     /locus_tag="KKO15_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30569..31510)
                     /gene="flgJ"
                     /locus_tag="KKO15_000912"
     CDS             complement(30569..31510)
                     /gene="flgJ"
                     /locus_tag="KKO15_000912"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBD6359801.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31510..32607)
                     /gene="flgI"
                     /locus_tag="KKO15_000913"
     CDS             complement(31510..32607)
                     /gene="flgI"
                     /locus_tag="KKO15_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBD6359802.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32619..33317)
                     /gene="flgH"
                     /locus_tag="KKO15_000914"
     CDS             complement(32619..33317)
                     /gene="flgH"
                     /locus_tag="KKO15_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBD6359803.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33370..34152)
                     /gene="flgG"
                     /locus_tag="KKO15_000915"
     CDS             complement(33370..34152)
                     /gene="flgG"
                     /locus_tag="KKO15_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBD6359804.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34290..35041)
                     /gene="flgF"
                     /locus_tag="KKO15_000916"
                     /pseudo
     CDS             complement(34290..35041)
                     /gene="flgF"
                     /locus_tag="KKO15_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(35061..36269)
                     /gene="flgE"
                     /locus_tag="KKO15_000917"
     CDS             complement(35061..36269)
                     /gene="flgE"
                     /locus_tag="KKO15_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBD6359805.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(36294..36989)
                     /gene="flgD"
                     /locus_tag="KKO15_000918"
     CDS             complement(36294..36989)
                     /gene="flgD"
                     /locus_tag="KKO15_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBD6359806.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37001..37404)
                     /gene="flgC"
                     /locus_tag="KKO15_000919"
                     /pseudo
     CDS             complement(37001..37404)
                     /gene="flgC"
                     /locus_tag="KKO15_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37408..37824)
                     /gene="flgB"
                     /locus_tag="KKO15_000920"
     CDS             complement(37408..37824)
                     /gene="flgB"
                     /locus_tag="KKO15_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBD6359807.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSFDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37979..38638
                     /gene="flgA"
                     /locus_tag="KKO15_000921"
     CDS             37979..38638
                     /gene="flgA"
                     /locus_tag="KKO15_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBD6359808.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38714..39007
                     /gene="flgM"
                     /locus_tag="KKO15_000922"
     CDS             38714..39007
                     /gene="flgM"
                     /locus_tag="KKO15_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBD6359809.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39012..39428
                     /gene="flgN"
                     /locus_tag="KKO15_000923"
     CDS             39012..39428
                     /gene="flgN"
                     /locus_tag="KKO15_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBD6359810.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39468..41003)
                     /gene="murJ"
                     /locus_tag="KKO15_000924"
     CDS             complement(39468..41003)
                     /gene="murJ"
                     /locus_tag="KKO15_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBD6359811.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41170..42036)
                     /locus_tag="KKO15_000925"
     CDS             complement(41170..42036)
                     /locus_tag="KKO15_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6359812.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(42038..42685)
                     /locus_tag="KKO15_000926"
     CDS             complement(42038..42685)
                     /locus_tag="KKO15_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBD6359813.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLYSLLAHLGD"
     gene            complement(42696..43280)
                     /gene="rimJ"
                     /locus_tag="KKO15_000927"
     CDS             complement(42696..43280)
                     /gene="rimJ"
                     /locus_tag="KKO15_000927"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBD6359814.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43516..44724
                     /gene="mdtH"
                     /locus_tag="KKO15_000928"
     CDS             43516..44724
                     /gene="mdtH"
                     /locus_tag="KKO15_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBD6359815.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44788..45435
                     /gene="grxB"
                     /locus_tag="KKO15_000929"
     CDS             44788..45435
                     /gene="grxB"
                     /locus_tag="KKO15_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBD6359816.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45569..46129
                     /locus_tag="KKO15_000930"
     CDS             45569..46129
                     /locus_tag="KKO15_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6359817.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46235..47281
                     /gene="pyrC"
                     /locus_tag="KKO15_000931"
     CDS             46235..47281
                     /gene="pyrC"
                     /locus_tag="KKO15_000931"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBD6359818.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47355..47600
                     /gene="dinI"
                     /locus_tag="KKO15_000932"
     CDS             47355..47600
                     /gene="dinI"
                     /locus_tag="KKO15_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBD6359819.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47890..48144
                     /gene="bssS"
                     /locus_tag="KKO15_000933"
     CDS             47890..48144
                     /gene="bssS"
                     /locus_tag="KKO15_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBD6359820.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48259..49377
                     /gene="solA"
                     /locus_tag="KKO15_000934"
     CDS             48259..49377
                     /gene="solA"
                     /locus_tag="KKO15_000934"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBD6359821.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49425..49538
                     /locus_tag="KKO15_000935"
     CDS             49425..49538
                     /locus_tag="KKO15_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBD6359822.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49799..50365
                     /locus_tag="KKO15_000936"
     CDS             49799..50365
                     /locus_tag="KKO15_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBD6359823.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50369..50944
                     /locus_tag="KKO15_000937"
     CDS             50369..50944
                     /locus_tag="KKO15_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBD6359824.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(50986..52038)
                     /locus_tag="KKO15_000938"
     CDS             complement(50986..52038)
                     /locus_tag="KKO15_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBD6359825.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52263..53183
                     /gene="lpxL"
                     /locus_tag="KKO15_000939"
     CDS             52263..53183
                     /gene="lpxL"
                     /locus_tag="KKO15_000939"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBD6359826.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53355..53552
                     /locus_tag="KKO15_000940"
     CDS             53355..53552
                     /locus_tag="KKO15_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBD6359827.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            53576..>54486
                     /locus_tag="KKO15_000941"
     CDS             53576..>54486
                     /locus_tag="KKO15_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6359828.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13306 a        14500 c        13451 g        13229 t
ORIGIN      
        1 cagtcaccag gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc
       61 gtggccgccc aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct
      121 ggcaacacgg ttatgaagcg acttctcttg cggacctcgt cgaagcaacc ggagccaaag
      181 cgcccacgct atacgcggaa tttaccaaca aagaggggtt atttcgtgcc gttctcgacc
      241 gctatatcga tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg
      301 tggagtctgc gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca
      361 ccccggcagg ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata
      421 tagccaatac gttgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat
      481 gtcaacgaca agcgcgcggg gaaatcccga cccactgtga cgtgacacat ctggcagaat
      541 tccttaattg tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa
      601 aactgatgca gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg
      661 caaaaaaggc ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta
      721 tactttgtta ttcaaaatca gttgcccgtt actatctagc gggatctgcg tacccggatc
      781 gcggtccatg cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag
      841 cattttttct gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc
      901 gctttcctgg agatagccct ggatctggtt accggcatat ccaccaccca gcgccccaca
      961 acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca
     1021 acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc
     1081 actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca
     1141 gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct
     1201 acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt attcacggga
     1261 acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga
     1321 cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg
     1381 aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag
     1441 ggcggataag gcgtttatac cacatccgcc agtgtacgtc gattaatgca acttcaaacg
     1501 cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc
     1561 atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc
     1621 ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt
     1681 cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt
     1741 catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg
     1801 cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat
     1861 gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt
     1921 ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat
     1981 cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt
     2041 gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc
     2101 agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt
     2161 cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg
     2221 caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt
     2281 gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa
     2341 gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt
     2401 gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc
     2461 cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc
     2521 acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc
     2581 cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag
     2641 tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg
     2701 tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat
     2761 cacaatcttt ttcaatggcg tagtcaacgt gatcccctta aatttattaa ccaattgtta
     2821 attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt
     2881 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
     2941 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagag tttattgttt
     3001 attatttgag caaattcgga agggtaatat agggtgaagc gtatatcgag ggcgtttggt
     3061 cgtcctgata agatgcgcag catcgcatca ggcattgatg ctcgactgtc ggatgcggcg
     3121 tggacgcctt atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga
     3181 tgcgacgagg gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag
     3241 atattcttga atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac
     3301 tcttcagatg tccgctggct gttaagtgct tcatcattac gcgacaaaat aaccagacaa
     3361 cgatcgcgat tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct
     3421 tccgggcgat caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc
     3481 acctggcgga tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc caggccaacg
     3541 ccgcaaatta gcggacgctc gtcaacgttc agttgcaaca ttttgtccac ttctttaagc
     3601 agatgctgca tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg
     3661 tcaataaact gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg
     3721 tgtaaataga taatcatcgc ttcctcacat aagccagggt tagtgacctg ctttctcttc
     3781 ctgccagcgt tcatgtaact gattcagacg ggtgctgatc gctttccagc gagccgagtc
     3841 catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt
     3901 gatcggtgac agattatcta acacgctgac ggccccttta cgattattgc agaccaggat
     3961 catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatcgc
     4021 ggcaccttcc atcgataaat cgtcagagaa aatcacgccg tcaaaaccca gttcctgacg
     4081 caaaacggtt ttcagccagt agggagaacc gctcgccgga cgcggatcaa catcactgta
     4141 gatcacatgc gcaggcataa tggcgtcgag tttattttcg cggattaagg aactgaagac
     4201 cgacatatct ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg tttctttgtg
     4261 tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc
     4321 ttcatgcata ccatcaataa accggctggc aattgccagg gctttttgtg gatcggcatg
     4381 ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca tccagcactg gcgcaaagct
     4441 gatatcaata tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag
     4501 tttgccgccc tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt
     4561 aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga tctaccgcca ccaccagatg
     4621 attgcgcgat gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata
     4681 gttacgcgta aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc
     4741 cgcgtccagt tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt
     4801 gttttattaa tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg
     4861 tttgtcgcca gcggtactca aaccaccctc ctttgagcat cagcagccag ggaaaccatc
     4921 gcctgacctg acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga
     4981 ccaattgccg gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat
     5041 caccatctcc agcatactcc cagtcgatga gttttaaccc tgatgcgcta tgcactaaat
     5101 ttccggcgtg gacatccata tgcaatggac ttaagcgtaa aggtcgtggt tcccgcgctt
     5161 tgcgcagacg ttttaacatt cgcagccaac ccacagtccg ccgcgccgga tcgctttgct
     5221 gccagtacag ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat
     5281 gtagataata cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcgc
     5341 cgggcagata gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc
     5401 ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag
     5461 gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc
     5521 cactaagccc actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca
     5581 attcgtcgcg cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt
     5641 tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag
     5701 gcgctggagt acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta
     5761 cgggtgccta aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagttgc
     5821 tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc
     5881 gcttctgcgg cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg
     5941 ctacccgcag tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca
     6001 ttccagtcat aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc gtgctcaatt
     6061 gggcctggct gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc
     6121 tcggctggtt gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc
     6181 acacacccgg cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc
     6241 atcaagattc acccctttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat
     6301 tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg cgtggccttt
     6361 ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa
     6421 cgggttcttg cctttcgtta taaagcgttg aggatgctga aggttgaata tcagacgtcg
     6481 aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg
     6541 attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaaca
     6601 ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat
     6661 tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc
     6721 atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc
     6781 aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc
     6841 gttcacagtc ggaatgagaa tattcggaat gatcagaata tgcgttggcg cttgcggcga
     6901 aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg
     6961 acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc
     7021 atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac
     7081 ctgctctttc aatcttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat
     7141 atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt
     7201 acacgacaaa tatgaatgcg tatatttctc atttgcattt gcatttgcag caaaattatt
     7261 cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg acaacataaa
     7321 ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat caagtcatca
     7381 ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct gccgcttttg
     7441 ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct ccagatgatg
     7501 gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag atgactcaac
     7561 gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat cagcagttac
     7621 aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag gcggattccg
     7681 atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt gatggtatcc
     7741 ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg gcactgtttg
     7801 aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt aatccatctg
     7861 cggcaattaa tatggttcga aaacacgcga cctgtcgtga atttaaaggc gatgtctcgg
     7921 cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc ccactcaccg
     7981 aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac tcatggctgg
     8041 accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat ttaggcgatc
     8101 ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc ccaacctggg
     8161 gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc gcacgcagta
     8221 ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg accctgaagc
     8281 agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa gtcgaatttg
     8341 acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg ctggttgggc
     8401 catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg aacagtggca
     8461 aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg tttggtcgtc
     8521 agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttca gttcatgggc
     8581 caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt tcccacaagc
     8641 cgactggtca tcacagagcc tggcgcagga cgataccaca catatgaaat cgttatatgc
     8701 tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac gttataccaa
     8761 ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc cttacgctgg
     8821 tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct ctattttcca
     8881 gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg gtaacaacta
     8941 cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt tagccatctt
     9001 ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg gcagcaacgg
     9061 cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat ttgaactcaa
     9121 cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctatc ttgcagagga
     9181 taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa tgttcaccag
     9241 ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact ggcaaaatcg
     9301 cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag gtagctacgc
     9361 gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac aggggaacgt
     9421 caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg tctacggcgc
     9481 accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg taaaaaaggc
     9541 agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac ggatgtactc
     9601 atccatctcg gttttcaggt tatcggattt agtaccgaaa atcgcctgaa caccagaacc
     9661 agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca ctttagacac
     9721 atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa tgttttcttt
     9781 accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg cttttgcatc
     9841 ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca gcacgcggaa
     9901 gatggtgtag taaacaatcg cataaccgat accgacgatc ggtaacagcc acagtttgct
     9961 gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg taccgtcacg
    10021 catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta
    10081 caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc tcggtgatac cggtcaggaa
    10141 cgaggtcagc gccgcggaga tcataatacc gcccactttc gcacggtttt ctggtttagc
    10201 agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca ggaagccacc
    10261 agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac
    10321 ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt tccagatgtg
    10381 gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa acgcaactac
    10441 cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa tcggcggcca
    10501 aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga tcggcacaaa
    10561 gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga aacggttaaa
    10621 catgtacgct gcaatcgcac cggagataat ccctccgagt acgccagtat ccgccaagtg
    10681 tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca cggccatggt
    10741 tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat cgttattggt
    10801 aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc
    10861 tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg cggaaccgac
    10921 gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg atttaccgac
    10981 cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg agtatgggtg
    11041 ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac agggcatcta
    11101 agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt tgacggctat
    11161 cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat atgtcacaat
    11221 atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc gttttagcca
    11281 ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg aagcgtcgat
    11341 gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa cggcaacacc
    11401 tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc
    11461 tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc gatccagggg
    11521 gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa
    11581 ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca
    11641 gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc
    11701 acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa tctggatatg
    11761 atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat aatccagccc
    11821 ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat cttctacatc
    11881 gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt tgtcacgttc
    11941 gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa
    12001 tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat gcaaagattc
    12061 ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa
    12121 gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat
    12181 cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca cgaatgtggc
    12241 aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc agttcggcga
    12301 ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc atgatggcgt tttagcgcat
    12361 ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga ttaagggccg
    12421 ccagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga cacaatgttt
    12481 cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc gaaccggcgc
    12541 ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt gaaagccaag
    12601 tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg
    12661 ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct
    12721 cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca gcatcggtta
    12781 cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac
    12841 gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg tagtaatcgg
    12901 gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt ttgtggccct
    12961 gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca tcgcccatgc
    13021 ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag ctggctacca
    13081 gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact ccttcaccca
    13141 gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg tggcatcaat
    13201 ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg tgcgattaaa
    13261 gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa
    13321 gccaacttcc ggggtaacat cgagatagag cgttaagtct gggcgaaaat ccccgagaac
    13381 agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc cctgatacgc
    13441 ctgagtggaa agatcgtggc gatcgccaat cacccaggtg ccgttagcca gcgcaggttt
    13501 gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt cggctttatc
    13561 ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta acttttcggc
    13621 aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca gttgctcgag
    13681 cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac
    13741 gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc acagacttgt
    13801 tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc acaaaataga
    13861 gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc ggcgtagcta
    13921 tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc gtttccaggt
    13981 ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg gttgggtcgg
    14041 tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt
    14101 cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc tgatttttat
    14161 ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
    14221 tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atcaaggtgt
    14281 ctggccagaa ccaaccttca atccactccg gattttccag ttcaagtgcc tgcgctacgg
    14341 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
    14401 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
    14461 tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
    14521 ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc cgtgggcgat
    14581 tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc cctggcttca
    14641 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
    14701 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
    14761 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
    14821 tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
    14881 aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt cgacaagctg
    14941 ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt taacacctgc
    15001 ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
    15061 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
    15121 aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
    15181 aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
    15241 gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc cgctgttcaa
    15301 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
    15361 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacaggaaat
    15421 catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca aactgacttt
    15481 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
    15541 tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc cttatcagag
    15601 aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc taagaaaaaa
    15661 ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt tttaaagatc
    15721 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
    15781 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg gttatccagg
    15841 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agattctact
    15901 gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
    15961 cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc gccagccggc
    16021 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
    16081 agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
    16141 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
    16201 ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag tacgaagcca
    16261 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
    16321 gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc tgcgccacct
    16381 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc aatgttgtgc
    16441 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
    16501 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
    16561 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
    16621 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc aaggccagaa
    16681 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
    16741 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
    16801 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
    16861 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
    16921 acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga ttctagtggg
    16981 acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt
    17041 ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg
    17101 gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat
    17161 ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca
    17221 gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc
    17281 tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac
    17341 taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac
    17401 gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat
    17461 tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg
    17521 tgatgtaagc tgcttcgtcg gatgctagga atgcaaccgc gttggcgatt tcctgtgcgc
    17581 cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg
    17641 cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg
    17701 acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt
    17761 agttggcctg accgccattt cccatggtac caaccacaga accgatagtg ataatacgac
    17821 catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa
    17881 ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac
    17941 gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt
    18001 ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg
    18061 cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt
    18121 tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg
    18181 ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc tcgagcgccg
    18241 ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg
    18301 tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg ccttgcgctg
    18361 ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt
    18421 cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca acagaaactg
    18481 ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca
    18541 tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt gcgccagcag
    18601 ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata accacctgtc
    18661 ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct tcacacgctt
    18721 tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg ccttccggta
    18781 cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca gcgaaatcaa
    18841 tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc atcattgccg
    18901 gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca gtcaacagcg
    18961 ctggctgggt ttgccaggtt ttattcagtt cttcagctgg cccctgctgg gtcagcgccc
    19021 acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc
    19081 tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg
    19141 caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag ccccaagtga
    19201 atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta
    19261 cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg
    19321 tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg ataatacgca
    19381 ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta ttcgccgcca
    19441 gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact tcgttgcccg
    19501 ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc agcgtcagca
    19561 attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc tcttcagagg
    19621 cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatctgttg
    19681 gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag
    19741 atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag gctgctgcaa
    19801 cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa gctgcgctcg
    19861 ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa
    19921 tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt
    19981 ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg
    20041 tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag
    20101 taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg
    20161 caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc
    20221 agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact
    20281 tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct
    20341 gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg
    20401 tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac
    20461 agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc
    20521 ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc acaaaccagc
    20581 acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa
    20641 gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt tacttcttct
    20701 tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccaga
    20761 actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac gttggcccct
    20821 aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac cagcgccgga
    20881 cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag tgccccggta
    20941 ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccatacgc
    21001 attgaactcc cacgactggc gcggatagct tgcgaaggcc gggcatcact ggcgataact
    21061 gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat
    21121 ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt
    21181 gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac
    21241 gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct
    21301 ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc
    21361 ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt
    21421 tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt
    21481 tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta
    21541 tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg
    21601 ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct tcggacactt
    21661 cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa
    21721 ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg
    21781 gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct
    21841 gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc
    21901 cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac
    21961 tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta tagataccct
    22021 ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct
    22081 tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct
    22141 gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag
    22201 tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa
    22261 tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccatg
    22321 gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac ctgaggtcga
    22381 cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa
    22441 agcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg
    22501 tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt
    22561 acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc
    22621 gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctaagcga
    22681 ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa
    22741 gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc ctaacacgct
    22801 ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct
    22861 gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg gtgcagacgg
    22921 cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt atatgcttgt
    22981 attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt
    23041 cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg gcgtgcagga
    23101 acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt
    23161 agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg atctgatgag
    23221 tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt tcttccactt
    23281 taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt tcgccgcttt
    23341 gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc
    23401 gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca tgcagagaac
    23461 gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg tcgagacgat
    23521 gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga
    23581 agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc accaggatat
    23641 gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa
    23701 ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt
    23761 cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct ttacgcaaaa
    23821 tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac
    23881 gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca
    23941 tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta tgcttatatt
    24001 tagttttgca ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc
    24061 accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
    24121 tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa
    24181 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
    24241 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
    24301 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
    24361 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
    24421 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
    24481 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
    24541 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
    24601 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
    24661 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
    24721 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
    24781 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
    24841 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
    24901 atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc
    24961 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
    25021 accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa
    25081 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct gattcatcag
    25141 gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat cggcgaaatc
    25201 cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc attaggtcgc
    25261 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
    25321 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc atccggtggt
    25381 tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc cgcacgcgcg
    25441 acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc cgccgatgag
    25501 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
    25561 atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
    25621 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgc
    25681 cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt
    25741 accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa
    25801 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct
    25861 tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt
    25921 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg
    25981 cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca tgaagaagcg
    26041 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcactg
    26101 gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc tgccgcgact
    26161 gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc acaacctggg
    26221 ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga agaagccaaa
    26281 ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
    26341 aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
    26401 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcaa
    26461 actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc
    26521 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
    26581 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
    26641 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
    26701 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
    26761 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
    26821 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
    26881 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
    26941 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
    27001 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
    27061 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
    27121 atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc gccagttgtt
    27181 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
    27241 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
    27301 accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc ggtggttgaa
    27361 ccgcaggaag agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct
    27421 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
    27481 gaaacggtgg ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
    27541 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcgtcacag tgactggcag
    27601 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
    27661 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
    27721 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
    27781 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
    27841 tcccagagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
    27901 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
    27961 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
    28021 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
    28081 gtttgttcag ctgcgtgcct aattccgcgc gaacggtaag gacattattc agcgagtttt
    28141 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
    28201 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
    28261 cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt
    28321 cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
    28381 ttccgccaac ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttataaccgg
    28441 caaaaatgta gcgcccatta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
    28501 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
    28561 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
    28621 cttccagtga cactttttga gtggcgaaag tacgtgccag cgtgtactgg ctgttttgcg
    28681 cctgtgcctg ggagagaaca acagcttgtg atgcagcaat gggatcgtct gaagggttaa
    28741 cgactcgctt acccgtcgac atctgctcgc cgtacttcat ccattctgcc tgagaattgg
    28801 tgatgccgcg catgttttgc tggtacatca tctgggtact gaaacgcatt ttatctcccc
    28861 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
    28921 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc
    28981 ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact
    29041 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
    29101 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
    29161 gttgcggttg tcgctatcac ccgcatcttc ttcgctcgcc atcgcgattt tcgcttcgtc
    29221 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
    29281 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
    29341 ggcatctggt gtcgccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
    29401 attatcgaac gagattttgt aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc
    29461 accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagt
    29521 aaagaaatct tcaccagcat cgccgttagc atcaaatccg gctttgtgtt ggctgttgaa
    29581 agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg
    29641 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
    29701 aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga
    29761 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggcatc
    29821 gtgatgttat aagtaccgcc atcctgaacg ctgacttcta cgccaacaat ctggtttaat
    29881 tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc tgcccccacg
    29941 cctgtcaggc gcgagatttg atcgttcagg ctggcaattt gtttagcgta attgttgatc
    30001 tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg cagatattga
    30061 tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc ctggcgcgcc
    30121 gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt
    30181 gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat ctgctcatag
    30241 cgggcggtca ggccgctact ttgcgtctgc gcagcacgta actggttggt aataaacgta
    30301 tcatactcac gctgcacacc agaaacgtag acgccattgc caacccagcc gccagcgccc
    30361 aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc aacgttatag
    30421 ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcgttcag tccgctcatg
    30481 gcgttattga tcaagctgga catggaggtt ccttataagc cttcaattac agagtattat
    30541 cggcagcgac ccgccggact tgagttattc agaacagatt atcaatgttc atgctgtagg
    30601 ttttgctcac cttgtcgctt atcgatttca tctgctggat catgttggtg agtttacggg
    30661 catagtgagg atcggtagca tagcccgcgt cctgtaggac ctgcgccccc tgttccgcac
    30721 tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca acgtaatccg
    30781 acaatgcttc cagatacgag ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc
    30841 cgttttcata ttcagtcgtg gtgatttcag tgacttgccc tttccagttg ccagaggctt
    30901 tgacaccaaa caggttatag cttggctcgc cgttttcgcg gcggatttgc cgttgccccc
    30961 aaccagattc aagcgcagcc tgagcgagga tcaaatgatg tggcacaccg ctttgctggc
    31021 ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tctactgtca cccggcagcg
    31081 aatcatcgta gttacgtggt acggcctttt gcaccagctg cgaaagcgtc tggttttgat
    31141 aacgcaccac ggtttcgagc gggaatttca tcggtgctgc tggcgtggac tcctctggca
    31201 atggttgttc tggcgtcatc tgtttaacca tcatctctgc aagccccaga cctttgcccg
    31261 ccgtcatctg ttgggcaatt tgctggtcat acatactggt atacagacga gtgtgctcgc
    31321 tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga
    31381 acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct tcgctcgctt
    31441 tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt agtttgctgt
    31501 cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt gcattgattg
    31561 caaaatagac atcagatcca tcggcgtagc gcccagcgca ttgagcgcac gcaccacgtt
    31621 attgaggctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat
    31681 ctgcgtttgt ggagtaacaa cagtctgtcc accaccaaac ggtgtatctg gctggctgac
    31741 attggcctga cgattaactg ttactgagag atttccctgc gctaccgcgc agctgtcgag
    31801 ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc
    31861 ctgcggggtg acattaacct gcatattctg gatatcggca aggaaacgga cctgggaact
    31921 gttgccactt ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg cgctgccata
    31981 tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt
    32041 aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggcaattcac gttcaataac
    32101 cgcaccattg gtgatccgtc caccgttcag ttggttaacc tgaacactgc taccgcctgc
    32161 ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca cctgactgtc
    32221 aacgccctta agcggtgtca tcaacaacgt acctccacgc aagcttttgg cattacccat
    32281 ggaagaaacc accacatcga tggtttgccc ctgacgtcca aacggaggaa gtgacgctgt
    32341 caccattacc gcagcgacgt tttttagctg catattggtg cccgtcggaa cggtaattcc
    32401 cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc
    32461 accagtgcca tccagcccca ctaccaagcc atagccaatc agtgagtttt gccttacccc
    32521 ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa ccagtagaag
    32581 aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga caggttaagg
    32641 aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt gcctacgtat
    32701 tcaatacgcg catccgccac ctgagtagac ggtacggtat tgctgccgct gatagtgcgt
    32761 ggattaacca cgcccgagaa gcgaataaat tcggtaccct gattaatggc aatctgtttt
    32821 tcacccacca catgcaggtt gccgttgacc agtacctggt caaccgtcac cgtcaacgtg
    32881 ccgctaaagg tattgctggc attggccccg ccctttccgt tgaacgtgtt accaccggag
    32941 gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg cacagtatca
    33001 aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt gctggcgctg
    33061 acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca
    33121 aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga accgttggcg
    33181 acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag cggcgtggag
    33241 ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc ataagtatgc
    33301 gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt aaaccggtga acctgccacc
    33361 ggttatgcct tagagttgcg tcagtttttg cagcatctga tcggtggtgg acaccgcttt
    33421 actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt ccgccacgtt
    33481 gacgttagac gtttcaacat acccttgata cagcagtccc gcgccgttca ggcccggcgt
    33541 gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag aggttttcgc caatgctctc
    33601 cagtccggta tcattcataa aggtggtgag attgagctgc ccaacctgaa ccggagctgc
    33661 ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac ttaacgcatt
    33721 cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gccgtcacca gctgcccgtt
    33781 ctgatccacc tggaaagagc cgtcacggat ataggctgat gagccatctg gcaacattac
    33841 ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttattggtct gcgacagatt
    33901 gccctggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc
    33961 ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg tttgataaag
    34021 cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt
    34081 gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg cgatccataa
    34141 tgaactgatc atataatgtc ctgtagtgga ttgctgacaa gcagagcaga agcacgcgac
    34201 agtgtgaatt tcccctcacc ctaaccctct ccccaaaggg gcgagggaat agtccgcgcg
    34261 cttttattgc aatgtcatca atcgttcaat taactcatcg acagcagttg gttggcacgg
    34321 cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg
    34381 gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag aacccccgac
    34441 atcacacgca aggttggatc tgcctgcagt accggccctc gcgtggcctg ggtttctgcg
    34501 cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt
    34561 ttcagacgcc ctactggcgc aactgtattt gccggatcgc ccggattgag cgccgagatt
    34621 gtgccatcgg cagcaatcgt gatttccgcc ccttccggca cagcaattgg ccccgcctcg
    34681 cctatcaccg gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca
    34741 ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccagccatcc tgctgcaacg
    34801 caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg
    34861 ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc actgcgcgca
    34921 aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta ctggcggtta
    34981 ccgcctgttg attcagcgtc tggctggctg ctcccatcgc agtataaatt gcgtgatcca
    35041 ttgagctatc ccgtcagcga ttagcgtaag ttaacccgcg tgttgaggat ctggtcctgg
    35101 gttttgatgg tctgggcgtt agacttatag ttacgctggg caacgatcat attgaccagt
    35161 tctttactga gatcgacgtt ggacgcttcc agcgcaccgt tggtcagggt gccaaagttt
    35221 cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg
    35281 tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc
    35341 agttgggttt gttcgttgga attgttgccg acaaccgtac cgtcatcatt gatttgataa
    35401 ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatgtt gttagcgccg
    35461 gtattttgct gcatggagtt gaggaagctc agactaaacg tggcgggttc tgcaccgtta
    35521 attgcgccgg ttgcgatatt attcgccatc gcaccatcca ctaatgtgcc attagcatta
    35581 aattccagtg ttgtcgctgt cttcgcaatg ctgtttggat cactgctatc ctgggtgtag
    35641 acctgccagt tattatcccc ggtcttcaca aaatagacgc tcatgtcatg agcattaccc
    35701 tgactgtcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt gctggcgctg
    35761 aatggcgtaa cagtaggaag cggatcactg gaattcaggt tgatctgcat cgacgccgtg
    35821 gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg attcgcccct
    35881 tgctgaatag tcggcggcgt accggttacc gggtaacccg tcagctgtaa accttgcgta
    35941 ttcaacaggt tacggttttc atccagctta aattgtccgt tacggctgta gaacaccgaa
    36001 ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc cagacctcgc
    36061 ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccggcaac ttttaccccc
    36121 agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg
    36181 taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc ggcagcgttt
    36241 aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa ggcttaaatt
    36301 atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag cgtattaccg
    36361 ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca gcggctgggc aaccagttgt
    36421 gtaccaccgt tactggcgct aatcgctaca ttgtaagaac cgttcggcgc agtgctgcca
    36481 tcagtcaacg aaccgtccca ggtgaaactg tgaactccgg cggtcagttc accaatatca
    36541 atggtgcgca caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt gtctgccgcc
    36601 tgttgcagct caacaccaaa cggcgtggtc gtggtcacag ccccttcttc actgccggtt
    36661 cccgcaagaa cagttgtgcc ggggatcatc acgccgtgac cgatcaggtt actggcctgt
    36721 aacgactggc tgttatcaat ctgtccggaa atagatccga gcgtggtatt gagtttttca
    36781 atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga
    36841 ttggtcgggt cctggttttt cagctgcgcc accagcaaag tcagaaaact gctttgtaaa
    36901 tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc ggtatttgtc
    36961 ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg agcgtaaggg
    37021 ttttcagcat catgcttttc accgtgttga gcacttcaac attggcctga tagctgcgtg
    37081 acgccgacat ggtgttaacc atctccccga cgacatcaac gttcggcatt tttacgtagc
    37141 cctttgcatc tgccagcgga ttacctggtt cataaaccag tttgtccggg gcctgacttt
    37201 cttaacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa
    37261 taccacctgt tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc
    37321 cagattactg gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat
    37381 atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc
    37441 tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa
    37501 ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca aacgaaggct ggtccggaat
    37561 acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga
    37621 ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag
    37681 ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt
    37741 tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa
    37801 ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct
    37861 taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc
    37921 cgttgatggt cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat
    37981 gctgacaata aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc
    38041 gagcaatctc acatcgcaat tgcacaactt ttttagcgcc caactcgcgg gggtaagtga
    38101 tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt
    38161 gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg
    38221 taacgacaaa cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc
    38281 gatgcctatt gtgcggggag gaaagctgga agctggcaat gtcaaactga aacgtggacg
    38341 cctggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag
    38401 cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg
    38461 ggtaaaagcg ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc
    38521 agaaggtcag gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc
    38581 gggacaggta gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc
    38641 tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa
    38701 tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca
    38761 accgcgcgaa accaccgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc
    38821 caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag
    38881 tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa
    38941 aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag
    39001 taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg
    39061 atctcaaaac ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca
    39121 gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg
    39181 aacagttacg caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc
    39241 aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac
    39301 tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac
    39361 cgacgctata tggggcgaac ggtcagacct caacacccca tcgcggcagt aaaaagattt
    39421 cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg
    39481 cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc
    39541 cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac
    39601 tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc
    39661 agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac
    39721 tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg
    39781 gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg
    39841 atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact
    39901 ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc
    39961 agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa
    40021 agaatgccca acgcaaccgc actcggcagt gccaacagga aacaaagacg caacccccag
    40081 tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc
    40141 agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg
    40201 gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg
    40261 attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg
    40321 cgcattgctc cagcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc
    40381 aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc
    40441 gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg
    40501 ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct
    40561 cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg
    40621 ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc
    40681 cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca
    40741 gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc
    40801 agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta
    40861 ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg
    40921 acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg
    40981 gccagcgatt ttaataaatt catcggtgtt ctaatccgga cccacgggca aaacgcctgc
    41041 aaggcaggcg ctgaaagagg cgaatagtct acctagatta tggtgaattg ctaccgccag
    41101 atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc
    41161 cagcactgcc tattcgccgg cggtttgcgg aactgtctga ttttgcacgc attcaatgaa
    41221 gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg
    41281 tttattcact acaccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat
    41341 gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat
    41401 acaagtggtg atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc
    41461 agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag
    41521 cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc
    41581 gacgctattg ctacgatgtt tatccattct tagcgaggct gcggtggcga gttgcgtttt
    41641 taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt
    41701 ccgcgccgcc agttccacca gcctttcagc atcgcgcaga ttttctgcca gcggtttatc
    41761 gacacagaca tgtacccccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt
    41821 gctggagtgc acaaaaaccg catcacaact ggcggcaagg ctggataacg aatcggcata
    41881 aggaatgcgc cagctgtcac aaattggcag ggctttggcg cgcgtaggcg accaggctcc
    41941 ttgtaacgtc cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc
    42001 gcctaatccc actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca
    42061 acgaataaag acgttgtttc agttctgcca cttcgatttc cagggcttcg acgcgggtct
    42121 gtaaatcacc gtcaaccatg ttcgacatag ccgttaccgc cggctgatct tcaacctcac
    42181 cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca
    42241 caaaaggacc atcttcgcga ttcgccagtt gttccagcgt cgactccact tccgccatat
    42301 cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac
    42361 gcaataacaa cgtggtgatt aacgccactt ctgcggcgct cagtttcaga tcgccaaatt
    42421 ctgaattaca aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc
    42481 gtagataatg acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat
    42541 tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga
    42601 gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatt acgcgagctt
    42661 ccagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg
    42721 gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc
    42781 gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc
    42841 ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct
    42901 gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg
    42961 agatagcagg catgaaaaga gccgcgaaca acattggaaa aattggcaac gccaataatc
    43021 tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg
    43081 ttaatcatcc ctagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact
    43141 ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgccag acgccaggca
    43201 tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg
    43261 ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt
    43321 aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca
    43381 ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt
    43441 ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat
    43501 ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg
    43561 ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc
    43621 cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc
    43681 cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc
    43741 aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc
    43801 gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg
    43861 tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt
    43921 tttttctcgc tgttgatgat gcaggacagt gccagtgcgg tcattggcgc attgttgggg
    43981 agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg
    44041 ctatgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg
    44101 cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg
    44161 acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc
    44221 aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg
    44281 tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac
    44341 cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc
    44401 ggcctacaac aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag
    44461 cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg
    44521 gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg
    44581 ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt
    44641 attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt
    44701 cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt
    44761 gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac
    44821 tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg
    44881 ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg
    44941 caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa
    45001 ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc
    45061 aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat
    45121 gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt
    45181 aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg
    45241 cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat
    45301 gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca
    45361 agccgcgttg ctaattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca
    45421 tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt
    45481 tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat
    45541 tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat
    45601 cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga
    45661 aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt
    45721 ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa
    45781 taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa
    45841 agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat
    45901 cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt
    45961 gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc
    46021 tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa
    46081 aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg
    46141 cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg
    46201 caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa
    46261 gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt
    46321 cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc
    46381 cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc
    46441 cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga
    46501 gctggagcgc ggatttaatg aagccgtgtt caccgctgca aaactttacc cggcaaacgc
    46561 aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg
    46621 catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga
    46681 catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct
    46741 gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg
    46801 tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa
    46861 ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa
    46921 tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg
    46981 tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg
    47041 cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct
    47101 gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa
    47161 cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac
    47221 tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata
    47281 aaaatcgtag cttcctgttg tcattaggtt attttatctg tataaataac cagtatattc
    47341 aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg
    47401 gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata
    47461 atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa
    47521 caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg
    47581 actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccggatcg ccccggcttt
    47641 tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca
    47701 atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag
    47761 aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga
    47821 agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag
    47881 ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac
    47941 atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat
    48001 aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc
    48061 agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg
    48121 gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg
    48181 gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat
    48241 gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc
    48301 gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat
    48361 atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat
    48421 ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg ggatgaactt
    48481 tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg
    48541 gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt
    48601 gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac
    48661 tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc
    48721 tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca
    48781 attcgtcatg acgatgatgg agtaactatt gaaacggttg acggtgagta tcaggcgaaa
    48841 aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag
    48901 cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa
    48961 ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa
    49021 aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa
    49081 cgtgttccgt ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt cttgcgcaat
    49141 gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct
    49201 gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct cattaccggc
    49261 ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg
    49321 caagaccaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca
    49381 tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc
    49441 actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc tggggattgt
    49501 tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg
    49561 acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgagatta ttcctgcttc
    49621 tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag attctttcat
    49681 tattaattca aaattattga aataaaaata aatatcattc aatttgttat ttttgtgttg
    49741 caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg atgcacttat
    49801 gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc attggctaag
    49861 cgcaattatt gtctacggca tgtttgcttt aggtctgtgg atggtcacgc tcagttatta
    49921 cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt tgttaatgat
    49981 ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc cgctgccgag
    50041 ttattcgccc atgactcgtc ttgccgcaaa agctggacat ctcgcattgt acctattgct
    50101 gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac cgatcagcgt
    50161 ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg cagactttgc
    50221 cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtctgtta tgcacggatt
    50281 tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca tgctgggaaa
    50341 gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc
    50401 gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg acaaagaagg
    50461 tcagcacgcc tttgttaact tccgcataca gcatcttggc tatagctggt tatatggcac
    50521 ctttaaagat ttcgacggta cttttacctt tgatgaaaaa aatccggctg ccgataaagt
    50581 gaatgtgaca attaacacca ccagcgtcga tactaaccac gccgaacgcg ataaacatct
    50641 tcgcagtgcg gattttctca ataccacaaa atatccacag gcaacattca cctccaccag
    50701 cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac
    50761 caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat ggggcggtaa
    50821 acgtgcaggc tttgaggccg aaggcaaaat taagctcaaa gacttcaata tcaagacaga
    50881 tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa
    50941 gtaagagtga aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc
    51001 aggaataccc agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt
    51061 ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca
    51121 aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag
    51181 gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca
    51241 atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt
    51301 gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc
    51361 gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaggcac tggccttttc
    51421 acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat
    51481 ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc
    51541 aaagtgcccc acttcatact catagtggtt acgcatgtcg ataaatagtg catcgggatc
    51601 gtcaagcatg gcgttcactt ccgccgcttg cagatactca ccaacattgc tggcatcaaa
    51661 gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt accttcatgc gcagtaccca
    51721 gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta aagccgggtc
    51781 gaaggcatag agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc
    51841 gttaatgccc tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg taaacagctg
    51901 atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat
    51961 ggtggttcgc ggttcgctct cagccaacat tttggctttt aacgcgtcgt tggaaatgcg
    52021 gttgtgtaac actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc
    52081 atgatatagc aattatcgat aattaacatc cacacatttt acgctacatt tgcgcattaa
    52141 aaattatttg ttatttacaa gcgcggcaat ttcgcccagt cttcagccac aaattttggt
    52201 tgcgggcgaa aaaatgcgac aatacataca attgcccgta taggttgaaa aacaggattg
    52261 atatgacgaa tctacccaag ttctccaccg cactgcttca tccgcgttat tggttaacct
    52321 ggttgggtat tggcgtactt tggttggtcg tgcaattgcc ctacccgatt atctaccgcc
    52381 tcggttgtgg attaggaaaa ctggcgttac gttttatgaa acgacgcgca aaaattgtgc
    52441 atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg
    52501 tgaagaattt cgaatccgtt ggcatgggcc tgatggaaac cggtatggcg tggttctggc
    52561 cggaccgccg aatcgcccgc tggacggaag tgatcggcat ggaacacatt cgtgacgtgc
    52621 aggcgcaaaa acgcggcatc ctgttagttg gcatccattt tctgacactg gagctgggcg
    52681 cgcggcagtt tggtatgcag gaaccgggga ttggcgttta ccgcccgaac gataatccac
    52741 tgattgactg gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca
    52801 aagatttaaa aggcatgatt aaagccctga aaaaaggcga agtggtctgg tacgcaccag
    52861 atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg
    52921 cgaccacgac cggaacctgg atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg
    52981 ttccacgccg taagccagat ggcaaagggt atcaattgat tatgctgcca ccagagtgtt
    53041 ctccgccact ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat
    53101 gcatcatgat ggcaccagag cagtatatgt ggttacaccg tcgctttaaa acacgcccgg
    53161 aaggcgttcc ttcacgctat taaatctccc atgccggatg cttcagaatg gcatccggca
    53221 ttaccacagc aaatccccct gatttagcga taaaagctct ctggattgcg ccccctggaa
    53281 gtcgggcgca taattagtgt gtttatcttt tcttcttatg ttcaccgcgc ctggcgcacc
    53341 aacagcggat tgctatgtca ccctgtgaaa atgacacccc tataaactgg aaacgaaacc
    53401 tgatcgtcgc ctggctaggc tgttttctta ccggggccgc cttcagtctg gtaatgccct
    53461 tcttacccct ctacgttgag cagcttggtg ttaccggcca ctccgccctg aatatgtggt
    53521 ccggtattgt cttcagtata atgggttcct aaacacatca ttttaggtat tgaatatgag
    53581 acgggcaatc agctacatca gattttcaag tgaacgacaa ctgaaagggg actctgtacg
    53641 ccgacaatct aagctggtta ctgactggct tgataagaac cctgagtttt acttggattc
    53701 atccttgagc tttaaagatt tggggaaaag tgcttttagt ggtaagcacc tgaaaggtgg
    53761 tctaggtgac tttttaaccg ctatcgagaa agggcttgtt aaagctggtg atacgctact
    53821 aatcgagagt ttagaccgtc tgtcacgtca ggatattgat attgcttctg agctattacg
    53881 ccgtatctta agggctggtg tagatgtagt cacactgagc gatggagaac attacacacg
    53941 agaaagttta aaagacccgt tagccctgat taaaagcatt ctgataatgc agcgggcgca
    54001 tgaggaaagt ttgagaaagt ctgagcgtgt ccaagctgca tggaaccgta aaaaagaact
    54061 catctccgaa ggtatcaagg tatctaggcg ttgtcctgca tggttaaggc tgaatgatga
    54121 ccgtcgaaca ttcacaatca ttcctgataa agtggaagtt gttaaacgag catttgacct
    54181 gagattacag ggtttaagct tttgggctat caccagaact ttaaacgatg aaggtcactt
    54241 gtcattgaat caatacacac ccaaacaaaa ggggtggtct gatactgctg taaagaaact
    54301 gctcagaaat cgtgctgtga ttggatgttt cacacctgct ggacgtgaag aagttcaggg
    54361 ttactacccc gcaataatca gtgagtcact attctatcga gtacagcaac tgaacacagg
    54421 gcaatatggt agggcttctg tatcaagtaa tccgctatca gtcaacttgt tcaggggtat
    54481 catcaa
//
LOCUS       DAECOD010000016        53366 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.016, whole genome shotgun sequence.
ACCESSION   DAECOD010000016 DAECOD010000000
VERSION     DAECOD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.016"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            187..480
                     /locus_tag="KKO15_000942"
     CDS             187..480
                     /locus_tag="KKO15_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD6359829.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KKO15_000943"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KKO15_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBD6359830.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="KKO15_000944"
     CDS             complement(1922..2374)
                     /locus_tag="KKO15_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBD6359831.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KKO15_000945"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KKO15_000945"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBD6359832.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="KKO15_000946"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="KKO15_000946"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBD6359833.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAKEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KKO15_000947"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KKO15_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBD6359834.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KKO15_000948"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KKO15_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBD6359835.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KKO15_000949"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KKO15_000949"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBD6359836.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KKO15_000950"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KKO15_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBD6359837.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KKO15_000951"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KKO15_000951"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD6359838.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KKO15_000952"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KKO15_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBD6359839.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KKO15_000953"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KKO15_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBD6359840.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="KKO15_000954"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="KKO15_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBD6359841.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="KKO15_000955"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="KKO15_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBD6359842.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="KKO15_000956"
     CDS             complement(15085..15582)
                     /locus_tag="KKO15_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBD6359843.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="KKO15_000957"
     CDS             complement(15579..15941)
                     /locus_tag="KKO15_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD6359844.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="KKO15_000958"
     CDS             complement(15931..16278)
                     /locus_tag="KKO15_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBD6359845.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="KKO15_000959"
     CDS             16387..16800
                     /locus_tag="KKO15_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359846.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="KKO15_000960"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="KKO15_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="KKO15_000961"
     CDS             complement(18324..20039)
                     /locus_tag="KKO15_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBD6359847.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="KKO15_000962"
     CDS             20206..21099
                     /locus_tag="KKO15_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD6359848.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="KKO15_000963"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="KKO15_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="KKO15_000964"
     CDS             complement(21910..23526)
                     /locus_tag="KKO15_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBD6359849.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="KKO15_000965"
     CDS             23821..24495
                     /locus_tag="KKO15_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6359850.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="KKO15_000966"
     CDS             complement(24534..26195)
                     /locus_tag="KKO15_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBD6359851.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLVLRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="KKO15_000967"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="KKO15_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBD6359852.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="KKO15_000968"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="KKO15_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBD6359853.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="KKO15_000969"
     CDS             27649..27981
                     /locus_tag="KKO15_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD6359854.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29233)
                     /locus_tag="KKO15_000970"
     CDS             complement(27983..29233)
                     /locus_tag="KKO15_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBD6359855.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            29334..30434
                     /gene="cbrA"
                     /locus_tag="KKO15_000971"
     CDS             29334..30434
                     /gene="cbrA"
                     /locus_tag="KKO15_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBD6359856.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30395..31732)
                     /locus_tag="KKO15_000972"
     CDS             complement(30395..31732)
                     /locus_tag="KKO15_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6359857.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31807..32955)
                     /gene="dgoD"
                     /locus_tag="KKO15_000973"
                     /pseudo
     CDS             complement(31807..32955)
                     /gene="dgoD"
                     /locus_tag="KKO15_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32952..33569)
                     /gene="dgoA"
                     /locus_tag="KKO15_000974"
     CDS             complement(32952..33569)
                     /gene="dgoA"
                     /locus_tag="KKO15_000974"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBD6359858.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33553..34431)
                     /gene="dgoK"
                     /locus_tag="KKO15_000975"
     CDS             complement(33553..34431)
                     /gene="dgoK"
                     /locus_tag="KKO15_000975"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBD6359859.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34428..35117)
                     /gene="dgoR"
                     /locus_tag="KKO15_000976"
     CDS             complement(34428..35117)
                     /gene="dgoR"
                     /locus_tag="KKO15_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBD6359860.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35395..36051
                     /locus_tag="KKO15_000977"
     CDS             35395..36051
                     /locus_tag="KKO15_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359861.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGCSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36097..36909)
                     /gene="yidA"
                     /locus_tag="KKO15_000978"
     CDS             complement(36097..36909)
                     /gene="yidA"
                     /locus_tag="KKO15_000978"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBD6359862.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37023..37421)
                     /locus_tag="KKO15_000979"
     CDS             complement(37023..37421)
                     /locus_tag="KKO15_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBD6359863.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37661..40075)
                     /gene="gyrB"
                     /locus_tag="KKO15_000980"
     CDS             complement(37661..40075)
                     /gene="gyrB"
                     /locus_tag="KKO15_000980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBD6359864.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40104..41177)
                     /gene="recF"
                     /locus_tag="KKO15_000981"
     CDS             complement(40104..41177)
                     /gene="recF"
                     /locus_tag="KKO15_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBD6359865.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41177..42277)
                     /gene="dnaN"
                     /locus_tag="KKO15_000982"
     CDS             complement(41177..42277)
                     /gene="dnaN"
                     /locus_tag="KKO15_000982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBD6359866.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42282..43685)
                     /gene="dnaA"
                     /locus_tag="KKO15_000983"
     CDS             complement(42282..43685)
                     /gene="dnaA"
                     /locus_tag="KKO15_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBD6359867.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43982..44062
                     /locus_tag="KKO15_000984"
     CDS             43982..44062
                     /locus_tag="KKO15_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359868.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44292..44432
                     /gene="rpmH"
                     /locus_tag="KKO15_000985"
     CDS             44292..44432
                     /gene="rpmH"
                     /locus_tag="KKO15_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBD6359869.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44449..44808
                     /gene="rnpA"
                     /locus_tag="KKO15_000986"
     CDS             44449..44808
                     /gene="rnpA"
                     /locus_tag="KKO15_000986"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBD6359870.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44772..45029
                     /gene="yidD"
                     /locus_tag="KKO15_000987"
     CDS             44772..45029
                     /gene="yidD"
                     /locus_tag="KKO15_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBD6359871.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45032..46678
                     /gene="yidC"
                     /locus_tag="KKO15_000988"
     CDS             45032..46678
                     /gene="yidC"
                     /locus_tag="KKO15_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBD6359872.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46784..48148
                     /gene="mnmE"
                     /locus_tag="KKO15_000989"
     CDS             46784..48148
                     /gene="mnmE"
                     /locus_tag="KKO15_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBD6359873.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            48390..48464
                     /gene="tnaC"
                     /locus_tag="KKO15_000990"
     CDS             48390..48464
                     /gene="tnaC"
                     /locus_tag="KKO15_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBD6359874.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            48685..50100
                     /gene="tnaA"
                     /locus_tag="KKO15_000991"
     CDS             48685..50100
                     /gene="tnaA"
                     /locus_tag="KKO15_000991"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBD6359875.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            50191..51438
                     /gene="tnaB"
                     /locus_tag="KKO15_000992"
     CDS             50191..51438
                     /gene="tnaB"
                     /locus_tag="KKO15_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBD6359876.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            51570..52745
                     /gene="mdtL"
                     /locus_tag="KKO15_000993"
     CDS             51570..52745
                     /gene="mdtL"
                     /locus_tag="KKO15_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBD6359877.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            52720..>53366
                     /gene="yidZ"
                     /locus_tag="KKO15_000994"
     CDS             52720..>53366
                     /gene="yidZ"
                     /locus_tag="KKO15_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBD6359878.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT        13136 a        14269 c        13477 g        12484 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc aaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgaa ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcacg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtattggt attggtattg gtattggtat tggtgagcag gtgattacgg ggagcaaagg
    29221 ttatctgttt catggcgcgg tcctgataca aaacggttcc cgccatcata cttcacaagg
    29281 acttcacttt cagacaatag tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac
    29341 atttcgacgt ggcgattatt ggcctcggcc cggcagggtc ggcgttggca cgaaagttag
    29401 ccggcaaaat gcaggtgatc gcgctggata aaaagcacca gtgcggtact gaaggtttca
    29461 gcaaaccttg cggcggtctg ctggcaccgg acgcgcagcg atcttttatt cgcgatggac
    29521 tgacgcttcc tgtcgatgtg atcgccaatc cacagatttt cagcgtcaaa actgtcgacg
    29581 tcgccgcatc gctcacgcgt aactaccagc gaagctatat caatattaat cgccatgctt
    29641 tcgacttgtg gatgaaatcg ctgatccccg ccagcgttga ggtttatcac gatagcctgt
    29701 gccgaaaaat ctggcgtgag gatgataaat ggcatgtcat ttttcgtgca gacggctggg
    29761 agcaacatat taccgcccgc tatctggtcg gtgccgatgg cgcaaactcg atggtgcggc
    29821 gacatctcta cccggatcat caaatccgta aatatgtcgc tatccagcag tggttcgcag
    29881 agaaacatcc ggtgccgttc tactcctgca tctttgataa tgcgataact gactgttact
    29941 catggagtat cagcaaagat ggttatttta tctttggcgg tgcctatcca atgaaagacg
    30001 gtcagacgcg tttcacgacg ctgaaagaga aaatgagcgc ctttcagttc cagtttggca
    30061 aagcggtgaa aagtgagaaa tgcacggtgt tgtttccctc acgctggcag gattttgtct
    30121 gtggtaagga caacgccttt ctgattggtg aagcggcggg atttatcagc gccagctcgc
    30181 tggaggggat tagctatgcg ctggatagcg cagagattct gcgtgcggtg ttcctgaaac
    30241 agccggagaa gagcaacgcc gcttactggc gcgccacaca caaactgcgt ttaaaactct
    30301 tcggcaagat agtaaaaagc cgatgcctga ccgcaccggc tttaagaaag tggattatgc
    30361 gcagtggtgt ggcgcatatt ccacagttga aagattatcc aacgcgcttc acatcaccca
    30421 ccagcaggat gtaagagagc gcgccaatca acgcgacggc ggagatataa accagtgcag
    30481 gcgcgaaacc gtaaccctgc gccaggtacc ccaccaccag cggaacggtg atgccgccca
    30541 gaccaccggc gaagttaaac acgccgccgg ttaaaccaat caggcgcatc ggtgccagag
    30601 aagagaccag cgaccaggta atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca
    30661 ggcacataat catcatcgga tcgttggtgt agttagcgcc cataatgcag gtggagatca
    30721 gcaagccgca gataatcggc gttttacgcg caaagcccag tgaaaagccc ttacgtacca
    30781 gcagatccgc gacccagcca gagagcagga cgccgacaaa cgccgcgagg aatggcaccg
    30841 tggtcataaa gcctgctttc agcgccgtga ttcctttttc ctgggttaaa tagttcggga
    30901 accaggttaa gaaaaaccac agtgcagaag ccaccgcaaa ttgcccaaga taaacgccga
    30961 tcagtttacg atggaacacc agtttccagt ctttggctgt taacggctga cgcgcctctt
    31021 tcttcaccgg cgcatcacca tccaccagac cgccgccatc acgaatgtaa tccagttcag
    31081 ctttgctgat acctttggtc aggcgcggcg gctgataaac cttaaaccaa atcagcgacc
    31141 agataatgcc gataccacca gtgacaatga acacccagtg ccagctcaac atctcctgaa
    31201 tccagatcag cagcggagtc aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac
    31261 caacggcgga agcgcgttca tgttccggga accagctggt caccatccgg ttattggtcg
    31321 ggaaagcggg cgcttcgaaa ataccggtta tcgcacgcag accaattaat gacattaatc
    31381 ccgtggcaaa gccttggaat aaagtcacca ctgaccaacc aaatatcgca ataaaataag
    31441 tcacgcgaga acctacgcga tctaaaaacc aaccgccggg gatctggcat agcgtataaa
    31501 gccaggcgaa ggccgaaaat acatagccca tttccgcttt cgtaatgccg aactcttcct
    31561 gaatatgggc ggaagccacg gccaggttag cgcggtcaac ataacaaatg accaccgtaa
    31621 taaagatcat caccagcgtc agataacgcc gacgccccgg ctttgctgca ttaacgggaa
    31681 tatccatagc gagctttctc cagattttgg gcatagcgaa gccgctcacc atgccctgta
    31741 atttacagag ggttatattt ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg
    31801 caggaattac cactctgcta cgctattatc ttcatgacgc cagagcggat tacgccagtc
    31861 cggggcattt ttactgaact caataacttt agcttcgtcg atttccacac ctaagcccgg
    31921 tttcgttaac ggtttaaaga agccgccaac catgctgaag tcttctttgt ttttcacaaa
    31981 gtcgagtaac tccgcgcctt tgttgtaatg aatgcccata ctttgttact gaagtaccgc
    32041 gttataggaa acaaagtcga tatgcaggca agccgccagt gcaatcggtc cgaacggaca
    32101 gtgcggcgca agggtcacat cataggcttc tgccattcca gcaattttgt agcattcggt
    32161 aataccgcct gcatgggaga gatccggttg cagaatcgaa ataccgcctg cctccagcac
    32221 gcgtttaaaa tcgaaacgtg agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg
    32281 tgccgccaat ttcgggtagt attcggcttg ttccgccagc accggctctt caataaacag
    32341 cgggcgatac ggctccagtt ctttaatcag cactttcgcc attggcgcgc tgacgcgacc
    32401 gtggaaatca agaccaaact caatctgatt gccaaaagct tcacgaattt gtgccacggt
    32461 gttgactgcc gcatctaccg cgcgggagtt atcaattagc cccagttctt cacaaccgtt
    32521 cagtttgaag gtatcgaagc cgatttcgcg cagggtttta atgccgtcga taacatccgc
    32581 cggacgatcg ccgccgaccc aactgtaggc tttaattttg tcgcgaacca ggccgcccat
    32641 cagttgccag actggcgcat tcagcacttt gcctttgata tcccataacg cctggtcgat
    32701 cccggcgatg gcgctcatca ggattggacc gccacgataa aatccggcgc gatacatcac
    32761 ttgccataag tcgttgatgc gcgaaggatc ctgaccaatc aaatagtcac ccagctcgtg
    32821 aactgccgct tccaccgtac gggcgcggcc ttcaatcacg ggctcgcccc aaccgaccac
    32881 gccttcatcg gtttcaattt tcaggaacat ccagcgggga ggtaaacgat acgtggtaat
    32941 tttggtgatt ttcattgcac tgcctctcga tacgccttaa caaatgctgc tgcctgctgc
    33001 gcggtgcgtt ctacggactg cccggcgcga taaagatcgc tgcctaagcc cgccccaaca
    33061 caacctgcgt ctatccactg cgccaggttt tctggcgtca cgccgccaac ggcaaagact
    33121 gcgatgtccg gtggtaatac cgcttttaac gctttgatgt attgcggacc aaaagccgat
    33181 gacggaaata ttttcagcgc ctgcgcgccc gcttcgagcg cggtaaaggc ttcggtcgcc
    33241 gtcgcgcagc cggggcaaac ggtcatgccg tagcccaccg cgcggcggat cacttcacta
    33301 tggatattgg gcgtaacgat gagctgacag cccatcctgg cgagcgcatc gacctgttca
    33361 ggtttcagta ccgtacctgc gccaatcaac gccttgtcgc catacgcatc aacgatggcg
    33421 ggaatgctct gctcccattg tggggaattc agcgggattt caaccgcgtc gaacccggcg
    33481 tcaatcaccg cgccaacatg cgccagcgcc tcgtcgggcg taatgccgcg caaaatggcg
    33541 atcagcggga gtttagtttg ccactgcatg agcgatgctc cttataccag cctgaaatgc
    33601 cgtgtcgccc gccaccgccg ccacgtcgca acccatcgcc tgaaaggctt gctggtagcg
    33661 cgcggtcagc gatgttccgg cgacaagggt gatggcgtgt tgatgggtca catagtcgcg
    33721 catactggca acctctgcgc caatcaacaa accagagaga aattcgctga cctgttcgcg
    33781 gggaagtgtt cccagcacat gcgaggcgcg aacttcaaaa agctgcggca atatggcggg
    33841 cgcattaagg ccgcgctcaa ggccagccgc gaaggcatcg gcagagtttt cctgcggcgg
    33901 caaacctgcg ccaatcaatg agtgatttaa cagtaaatga tgtaattcac cggtcatcac
    33961 ggtgcgaaaa tcgttgattt gctggctatc ggcctgcacc catttgcaat gcgtaccggg
    34021 cataacataa agagaggaag gagccagagc tcgcgcgccg atcaattgtg tttcttcgcc
    34081 gcgcatcaca ttgtggttat cgtcatgaga gacgcataat ccgggaataa tccagatatt
    34141 gtcgccaaca gacgttaatt gttcgccaat agacgaaaaa cgggcaggaa cagataaata
    34201 cggagcaact ttccagccga cattgctgcc gaccattcct gccatcacca ctggcgtatt
    34261 ttcttcacgc cagtcggtcg tgacttctgc taacaccgca gccggagatt ttccgttcag
    34321 gcgcgtgacg cctgcttctg attgcctgct ctccaggcag tggtcgccct gataaagcca
    34381 ggcgcgcaga ttggtcgatc cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc
    34441 tttaaccttc gtgtcgagct ggcaatcatg gtaagcgccg cctgctctgc cgcatcgccg
    34501 tcctgatgcc gtatcgcatc gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg
    34561 gcctcatcgc ccatccaggt tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg
    34621 ctaagttgct gtaacaccgg gttatgcacc gactgcagca ccgcctcgtg gtagcgaata
    34681 tccgcttcgt taaacgcttc gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat
    34741 tcaatctgcg ccagatcgct ggaggtcgcg cgctctgctg cccaacgggc aatcgccggt
    34801 tccaccagat ttcgcacttc gctcatggca ctgataagcc gtgggtcgta gtcgttttcc
    34861 agcacccatt gcagtacgtc agtgtcgagg taattccact ggttacgcgg tgccacaaac
    34921 gccccgcgat aacgtttcat ttcaatcagc cgcttcgcca tcagcgaacg gaacacctca
    34981 cggatgatgt tgcgcgaggt tgcaaactct tcacagagct ccgcctcagc cggaagtggc
    35041 gagccaggta cgtatttgcc gtgaacgatc tgtttaccca gcgtaatgac aatgcgatcg
    35101 gttttattga gagtcatgga gagtccttgt gctctgtatg ttcttctcta ctttaccccg
    35161 atcgatgcat aacgcggcaa ctttgtagta ccagcgtgat gacgttcgcg tttgccgcgc
    35221 gcgtaatgta gtacaaactt atattgttgt gctacaattt agatcaaaaa aagaacaata
    35281 cataaaaaaa tgacatgcgt cgggcagaaa tctgaaaagg gatatcaggc gctaaacagg
    35341 agggaaagaa gagtatgctt tcaacggctt agctactcgt ttaaaggatt aatcatgaag
    35401 ttgaatttta agggattttt taaggctgcc ggtttattcc cactggcgct gatgctttca
    35461 ggctgtatct cgtatgctct ggtttcccat accgcaaagg gttgttcagg aaagtatcaa
    35521 tcgcagtcag acaccatcac tgggctatcg caggcaaaag atagtaatgg aacaaaaggc
    35581 tatgtttttg taggggaatc gctggattac cttatcactg atggtgccga tgacatcgtt
    35641 aagatgctca atgatccagc acttaaccgg cacaatattc aggttgccga tgacgcaaga
    35701 tttgttttaa atgcggggaa aaagaaattt accggcacaa tatcgcttta ctactactgg
    35761 aataacgaag aagaaaaggc actggcaacg cattatggtt ttgcctgtgg tgttcaacac
    35821 tgtaccaggt cactggaaaa cctaaaaggc acaatccatg agaaaaataa aaacatggat
    35881 tactcaaagg tgatggcgtt ctaccatcca tttaaagtgc gattttatga atactattca
    35941 cccagaggca ttccggatgg tgtttccgca gcattactgc cagtgactgt tacactggac
    36001 atcattactg caccgctgca atttctggtt gtatatgcag taaaccaata atcagtaagc
    36061 gggcaaacgc gtttatgctg tttgcccgcc cacagattaa ttcagcacat acttctcaat
    36121 agcaaacgcc acgccatctt caaggttgga ttttgtgaca aagttcgcca cttctttcac
    36181 cgaaggaata gcgttatcca tcgccacacc gacgcctgca tattcaatca ttgcgatgtc
    36241 gttttcctga tcgccaatcg ccatgatttc ttccggttta atacctaaca cgtcggccag
    36301 tgatttcaca cccgtacctt tgttaacgcg tttatcgagg atttcgagga agtacggcgc
    36361 acttttcagc acggtatatt tctctttcac ttcctgcgga atacgcgcga tagcctggtc
    36421 gaggatggcg ggttcatcaa tcatcatcac tttcaggaac tgggtattgg ggtccatttt
    36481 ctccgcttcg cagaacacca gcggaattgt ggcaacgaag gattcatgca ccgtgtagta
    36541 gctgatatcg cggttagcgg tgtacagcgt ggtgcggtcc agggcgtgga aatgagaacc
    36601 gacttcgcga gagagttttt ccaggaaacg atagtcgtca tagctgagag cggtttgcgc
    36661 cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc
    36721 gcccggctgt tccatatgca gctctttcag gtagttgtgc acacctgcat acggacgacc
    36781 cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc
    36841 gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt
    36901 gatagccatg agttcccaag gtagattagt tcctgaccca tgctaacgcg attccgctca
    36961 aaaatcagta caacacccga gggaaaaggg gatgcaacgc gggttgcatg ctccccattt
    37021 gcttagcgga agagtttccc tttcagcagt tccatgcctg cggaaagcag atcgttattg
    37081 gcttgtgctg acacttcacc ttgcggtgag agcgcatcaa taatcttcgg caattgctct
    37141 gccagtaaac tggaagccgt actggtatcc acgccaagtt tttgcccgag atcggacacc
    37201 gcatttgtgc cgagcgccga ctccagttgc tcgccgctaa ctgattgatt tctctgttga
    37261 ttactcaacc aggttgagag aatggcccct aagccgccac tttgcagttt ttccagcagc
    37321 acctgaatgc caccctgctc ctcaacccaa cttaaaatcg cctgatattt ccccgcatcg
    37381 cctttcagaa aggcaccgac aacttcatca aaaagcccca tgataatcac ctgtaaagcg
    37441 ttacgtgttg acccaaaaag tatagatctg cgggatgata attgcggatt gcagaaataa
    37501 aaagggcgga gatgatctcc gcccttttct tatagcttct tgccggatgc ggcgtgaacg
    37561 ccttatccgg cctacaaaat catgcaaaat caatatattg caggagtctc gtaggactaa
    37621 taagcgtagc gcatcaggca cgctcgcatg gttaacgcca ttaaatatca atattcgccg
    37681 ctttcagggc gttctcttca ataaacgcac gacgcggttc tacggcgtcg cccatcagcg
    37741 tggtgaacaa ctggtcagcg gcaatcgcat ccttaacggt aacgcgcagc atacgacggc
    37801 tttccgggtc catggtggtt tcccacagct gttccgggtt catctcgccc agacctttat
    37861 aacgctggat ggagaggcca cgacgggact ctttcaccag ccagtccagc gcctgctcga
    37921 agctggctac cggctgacga cgctcgccac gttcgataaa cgcatcttct tccagcaagc
    37981 cacgcagttt ctcacccagc gtgcagatac gacgatattc gccaccggtg ataaactcgt
    38041 gatccagcgg atagtcagta tccacaccgt gggtacgcac gcgaacaatc ggctcgaaca
    38101 ggttttgttc ggcattggtg tggacatcaa acttccactg gctgccgtgc tgttctttgt
    38161 cgttcagttc gctgaccagc gcgttcaccc agcgggtaac ggtctgctca tcagaaaggt
    38221 cagcttccgt caacgtcggc tgatagataa gctctttcag cattgctttc ggataacgac
    38281 gctccatgcg gttgatcatt ttctgcgtcg cgttgtactc agataccagt ttctctaacg
    38341 cttcgccagc caatgccggt gcactggcgt tggtgtgcag cgttgcgccg tccagcgcga
    38401 tagagatctg gtactgatcc atcgcttcgt cgtctttaat gtactgttcc tgcttgcctt
    38461 tcttcacttt gtacagcggc ggctgagcga tgtagacgtg accgcgttca acgatttccg
    38521 gcatctgacg atagaagaag gtcaacagca gcgtacgaat gtgcgagccg tcgacgtccg
    38581 catcggtcat gatgatgatg ctgtgataac gcagtttgtc cgggttgtac tcgtcacgac
    38641 cgataccaca gccaagcgcg gtgataagcg tcgccacttc ctgagaagag agcatcttat
    38701 cgaagcgcgc tttctcgacg ttaaggattt tacccttcag cggcagaatc gcctggttct
    38761 tgcggttacg cccctgcttc gcagagccgc ccgcggagtc cccttccacc aggtacagtt
    38821 cggaaagcgc cggatcgcgt tcctggcagt ctgccagttt gcccggcagg ccagctaagt
    38881 cgagcgcacc tttacggcgg gtcatttcac gcgcgcgacg cgccgcttca cgggcacggg
    38941 cagcatcgat aattttgcca accacgattt tcgcatcagt tgggttttcc agcaggtatt
    39001 ccgccagcag ttcgttcatc tgctgttcaa ccgccgattt cacctcagaa gaaaccagtt
    39061 tgtctttggt ctgggaggag aatttcgggt ccggcacttt tacggaaacg accgcaatca
    39121 ggccttcacg cgcatcgtca ccggtggcgc tgactttggc ttttttgctg tagccttctt
    39181 tgtccatgta ggcattcagg gtacgggtca tcgccgcacg gaagcctgcc aggtgagtac
    39241 cgccgtcacg ctgcggaatg ttgttggtaa agcagtagat gttttcctgg aagccatcgt
    39301 tccactgcag cgccacttca acgccaatac cgtctttttc ggtggagaag tagaagatat
    39361 ttgggtggat cggcgttttg ttcttgttca gatattcaac gaacgctttg atgccgcctt
    39421 catagtggaa gtggtcttct ttgccgtcac gcttgtcgcg cagacgaatg gaaacgccgg
    39481 agttgaggaa cgacaactca cgcagacgtt tcgccagaat ttcatattcg aactcggtca
    39541 cattggtgaa ggtttcgagg ctgggccaga aacgcaccat ggtgccggtt ttttcagtct
    39601 cgccggtaac cgccagcgga gcctgcggta caccgtgttc gtagatctga cggtgaattt
    39661 taccctcgcg ctgaataacc agctccagtt tttgcgacag ggcgtttact accgaaacac
    39721 caacgccgtg cagaccgccg gacactttat aggagttatc gtcaaattta ccgcctgcgt
    39781 gcagaacggt catgatcact tccgccgccg atacgccctc ttccgggtga ataccggtcg
    39841 gaatgccgcg cccgtcatcc tgtacagaga cagagttatc ggcgtgaatg gtgacgataa
    39901 tttctttaca gtgacccgcg agcgcttcgt cgatagcgtt atctaccacc tcgaatacca
    39961 tgtggtgcag accggtgccg tcatccgtgt cgccgatata catacccggg cgcttacgca
    40021 ccgcatccag ccctttcagg actttgatac tggaggagtc ataagaattc gacatcaacg
    40081 tttctcgctc atttatactt gggttaatct gttattttac ccttttccac ggtaaacatc
    40141 ttcgaatttt cgtccgacat gtctataacg tgttcagcac tgatcgcgct gacaaagacc
    40201 tgtgattgcg tcgcttttaa gcggctggca agcaacccgc gacgctcatc atcaagctca
    40261 gaggcaaaat catctatcag gtagagacac cgccgcccgc tttcacgagt gaggaactct
    40321 ccttgcgcca gacgtaaggc gcacatcaac agcttaagct gcccacgcga taaggtatct
    40381 tccaccggcg caccgtcggc gcgaatgcgt aagtccgctt tatgcgggcc atgcgcggtg
    40441 taggttagct ggcgatcgcg ttcaaaatta cgttccagca cctcagcgta ctctgtctct
    40501 ttctcccagc cgcgctggaa agagaaagtc agagaaaact cagggagaaa ttgcttacag
    40561 gtatcatcca tatcggccgc gataccggcg ctatactccg cgcgccaggt gctgatttgc
    40621 tccgccagcg ggatcagttc tttatcccac gggcgtagct gttcgtaacg agtcacctgg
    40681 cgcagcgccg cattgcgctg cttgagcaat cgcttgagat tgctccaggc ggtgaaaaat
    40741 ccaggttcgt tgtgaaagca tccccagtcg aggaatgctc ttctgtattt ggggccgccg
    40801 ttgagtaaag taaacccttc tggcgttatc aactgcatcg gcatcaggtg cgccagttcc
    40861 gcgactttat gcccgtccgt accgtcaata cggactttgc tgtcgccctg tttgtctttg
    40921 gttaagccaa tcgccgtctc gcgctcttcg ccctgtaatc gcccgtggag aacaaatgcc
    40981 tcctgctcat ggcgaatgac gcgaccaatc tgcaaactgc gaaacgcccg accatggccg
    41041 agcgtataga tggcttccag cacgctggtt ttgccactgc cgttggcacc taccagaaag
    41101 ttaaagccgg gagataaagc gagatccgcg gtttcaatat tgcggaaatc gcggatcaac
    41161 aagcgggtga gggacattac agtctcattg gcatgacaac ataggcagcc gactgtgatg
    41221 cggcatcttc aatctgcacg ctggaaaccg aatcggtcag catcatgcgg acgttttcgc
    41281 atttcagcgc gttcagaaca tccagcacat agctgacgtt gaagccgatt tccatctccg
    41341 caccgctata ggtaacgtcg aggatctctt ccgcttcttc ctgttccggg ttgttggcag
    41401 taattttcag ctggttttcg ctgacataca ggcgcacacc gcggaatttc tcgttagaga
    41461 gaatcgccgc gcgagcaaac gcctgcttga gcagatcgca gcctgcttcc agatgtttgt
    41521 ccgggttctt cggcagaacg cgacggtaat ccgggaagcg accatccacc agtttcgagg
    41581 tgaaaataaa gtcgccaacg tgggcacgaa tgttattgct gccaatctgc acacgcagca
    41641 gattgtcgcc gccgtcgagc atacgcatca gttcaatcac gcctttacgc ggtacgatca
    41701 ccgaatggct tggcaaagat tgaccaattg gcattgaaca gaccgccaga cggtggccgt
    41761 cggttgccac ggtgcgcagt tcttcacctt cggtttcaaa cagcataccg tttaagtaat
    41821 agcgaacatc ctgatgcgcc atcgaaaact gggttgcttc aatcagacgc ttcatcgtcg
    41881 cctgcggcag ggtaaattcg acttcgctct gccagtcatc gaggttcggg aaatccgccg
    41941 ctggcagggt agacagcgaa aaacggctac gcccggagcg caccagcatc cgttcacctt
    42001 ccagctgcac ggcaatttcc gcgccttcag gcagaccacg gcagatatca aagaatttgc
    42061 gcgccggaac ggtcgtcgct cctggctcgt gtggctgaac cagcgcaacg cgtgccacca
    42121 tttccatctc gagatcagta ccggtcagcg acaacgtacc gtcagcaacc tgtaacagca
    42181 gattaccgag aatcggtagc gtaggacgac cacctaacgg accgctcacc tgttgtagcg
    42241 gttttaataa atgctcacgt tctacggtaa atttcatagg tttacgatga caatgttctg
    42301 attaaatttg aaaaatcttc tttgatatcg tggctctctt cacgcaactg ctcgatctta
    42361 cggcaggcat gaagcaccgt cgtgtggtca cgcccaccaa acgcatcgcc aatctctggc
    42421 agactgtggt tagtcagctc tttcgctagc gccatcgcca tctggcgcgg acgcgccacc
    42481 gagcgggatc gacgcttgga aaggagatcc gcgactttga tcttgtagta ctccgccacc
    42541 gtcttctgaa tattgtcgat ggtgaccagt ttttcctgca atgccagcaa gtcgcgcagc
    42601 gcctcacgca cgaagtcgat ggtgatcgcc cgtccggtaa agttggcgtt agcaataacg
    42661 cggttcagcg ccccttccag ctcacgtacg ttagatcgta gacgcttggc gataaagaac
    42721 gccacttcac ccggcaaacg aatgtcattt tcgtcggcct ttttcatcag gatcgccaca
    42781 cgggtttcca gctctggcgg ttcgatcgcc acagtcagtc cccaaccgaa gcgggatttc
    42841 aaacgatcct caacgccgtt gatctctttc ggatagcgat ccgaggtgag aatgatctgt
    42901 tgattacctt ccagcagggc gttgaaggtg tggaaaaact cttcctgaga tcgttcttta
    42961 ttagcaaaaa actgaatatc gtcgatcagc agtgcatcta cggaacggta gtagcgttta
    43021 aactcttcga tcgcgttgtt ttgcagggct ttaaccatgt cctgaacaaa gcgctcggag
    43081 tgcatataaa ccactttggc attcggcttg cgcgccataa tgccgttacc caccgcatgc
    43141 agcaggtgag ttttacccag acccgtgccg ccataaagga acaacgggtt ataggcaccg
    43201 ccagggttat ccgccacctg gcaagccgcc gcgcgcgcca gttggttaga tttaccttca
    43261 acgaagttat caaacgtgtg tttgacgttt acgttagaac gataggtcgg ttctgccggg
    43321 gccgggacgt tatcccaacc tgagcgcgta gaaggcgcag cacgttgcgg ctgcgtttgc
    43381 gccacctgtg caggggccgc gacgttgctc gtcactgccg cttgtggcgt ttgcgtcacc
    43441 ggtttggtgc cgacttcaaa acgcagctgt ggggcatccg ctccgcagaa actggttagc
    43501 agtccattga tattattaag gtacttgtcc cgtacccaat cgaggacaaa acggtttggc
    43561 gcgtacaggg ccagcgtgtt atcgctcagt tccgcctgca atgggcgtat ccacatactg
    43621 aattctgtgg ctggtaactc atcctgcaat cgggcaagac actgctgcca aagcgaaagt
    43681 gacacggcgg actccactcg aacaaaagtc gataatgact aaggctgaaa cattcatgat
    43741 tgttgacgta cgtcgaaaag accctgcttg agggtgacgc acgaaccgct gtctgcggtt
    43801 atatgcccga tcaagatcct gcaaaacgat cgggaccgcg gatcatagcc taaactgcgc
    43861 aagagatctt ctgtttctca cagattttcc ctatttatcc acaggacttt ccagaactcg
    43921 ctaagtgtaa acgatcctgc cgcgaggcgg gcacgattta cgccgcatat tggaaaattt
    43981 aatgaccata gacaaaaatt ggcttaatcg atctaataaa gatccaggac gatccttgcg
    44041 ctttacccat cagcccgtat aatcctccac ccggcgcgcc atgctggttt ccactggtgt
    44101 gaggtcgtac attttccctg cgaaaaggtg cggaaaagcg cggtaaataa ggaaagagaa
    44161 ttgactccgg tgtgtacaat tattacaatc cggcctcttt aatcacccat ggcttcggtg
    44221 tccatcgttt catttttcgg cggatatcca ataaagccat tgaatttatt caagtttagg
    44281 tagaaatcgc catgaaacgc acttttcaac cgtctgtact gaagcgcaac cgttctcacg
    44341 gcttccgtgc tcgtatggct actaaaaatg gtcgtcaggt tctggcacgt cgtcgtgcta
    44401 aaggccgcgc tcgtctgacc gtttctaagt aataaagcta acccctgagt ggttaagctc
    44461 gcatttccca gggagttacg cttgttaact cccagtcaat tcacattcgt cttccagcag
    44521 ccacaacggg ctggcacgcc gcaaattacc attctcggcc gcctgaattc gctggggcat
    44581 ccccgtatcg gtcttacagt cgccaagaaa aacgttcgac gcgcccatga acgcaatcgg
    44641 attaaacgcc tgacgcgtga aagcttccgt ctgcgccaac atgaactccc ggctatggat
    44701 ttcgtggtgg tggcgaaaaa aggggttgcc gacctcgata accgtgctct ctcggaagcg
    44761 ttggaaaaat tatggcgccg ccactgtcgc ctggctcgcg ggtcctgata gccctcattc
    44821 gggtctatca acgcctgatt agtccgctac tcgggccgca ttgtcgtttc actccaacct
    44881 gttcaagcta cggaattgag gcattgcgca ggtttggagt gataaaaggc agttggttga
    44941 cggtgaaacg cgtattaaaa tgccaccctt tacaccctgg tggtgacgat cccgtcccgc
    45001 ccggaccatt tgataccaga gaacactaac gatggattcg caacgcaatc ttttagtcat
    45061 cgctttgctg ttcgtgtctt tcatgatctg gcaagcctgg gagcaggata aaaacccgca
    45121 acctcaggcc caacagacca cgcagacaac gaccaccgca gcgggtagcg ccgccgacca
    45181 gggcgtaccg gccagtggcc aggggaaact gatctcggtt aagaccgacg tgcttgatct
    45241 gaccatcaac acccgtggtg gtgatgttga gcaagctctg ctgcctgctt acccgaaaga
    45301 gctgaactct acccagccgt tccagctgtt ggaaacttca ccgcagttta tttatcaggc
    45361 acagagcggt ctgaccggtc gtgatggccc ggataacccg gctaacggcc cgcgtccgct
    45421 gtataacgtt gaaaaagacg cttatgtgct ggctgaaggt caaaacgaac tgcaggtgcc
    45481 gatgacgtat accgacgcgg caggcaacac gtttaccaaa acgtttgtcc tgaaacgtgg
    45541 tgattacgct gtcaacgtca actacaacgt gcagaacgct ggcgagaaac cgctggaaat
    45601 ctcctcgttt ggtcagttga agcaatccat cactctgcca ccgcatctcg ataccggaag
    45661 cagcaacttc gcactgcaca ccttccgtgg cgcggcgtac tccacgcctg acgagaagta
    45721 tgagaaatac aagttcgata ccattgccga taacgaaaac ctgaacatct cttcgaaagg
    45781 tggttgggtg gcgatgctgc aacagtattt cgcgacggcg tggatcccgc ataacgacgg
    45841 taccaacaac ttctataccg ctaatctggg taacggcatc gtcgctatcg gctataaatc
    45901 tcagccggta ctggttcagc ctggtcagac tggcgcgatg aacagcaccc tgtgggttgg
    45961 cccggaaatc caggacaaaa tggcagctgt tgctccgcac ctggatctga ccgttgatta
    46021 cggttggttg tggttcatct ctcagccgct gttcaaactg ctgaaatgga tccatagctt
    46081 tgtgggtaac tggggcttct ccattatcat catcaccttt atcgttcgtg gcatcatgta
    46141 cccgctgacc aaagcgcagt acacctccat ggcgaagatg cgtatgctgc agccgaagat
    46201 tcaggcaatg cgtgagcgtc tgggcgatga caaacagcgt atcagtcagg aaatgatggc
    46261 gctgtacaaa gctgagaagg ttaacccgct gggcggctgc ttcccgctgc tgatccagat
    46321 gccaatcttc ctggcgttgt actacatgct gatgggttcc gttgaactgc gtcaggcacc
    46381 gtttgcactg tggatccacg acctgtcggc acaggacccg tactacatcc tgccgatcct
    46441 gatgggcgta acgatgttct tcattcagaa gatgtcgccg accactgtga ccgacccgat
    46501 gcagcagaag atcatgacct ttatgccggt catcttcacc gtgttcttcc tgtggttccc
    46561 gtcaggtctg gtactgtact atatcgtcag caacctggta accattattc agcagcagct
    46621 gatttaccgt ggtctggaaa aacgtggcct gcatagccgc gagaagaaaa aatcctgatt
    46681 cggtgagttt tcgctaaaat aagggcggtc agttgaccgc cttttttctt ttcgtagggc
    46741 ggataagcac cgcgcatccg ccacacaaag caacaggaac atcatgagcg ataatgacac
    46801 tatcgtagcc caggccacgc ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg
    46861 cttcaaagcc cgtgaagttg ccgaaaccgt gctgggtaaa ctgcctaagc cgcgctacgc
    46921 cgattatctt ccgtttaaag acgccgacgg cagcgtgctc gatcagggga ttgcgctatg
    46981 gttccctggc ccgaactcgt tcaccggcga agatgtgctg gaactgcaag gtcatggcgg
    47041 tccggtgatc ctcgacctgc tgttaaaacg cattctgacc attcccggcc tgcggattgc
    47101 tcgccctggt gagttttccg aacgcgcgtt tcttaacgat aaacttgact tagcccaggc
    47161 cgaggcgatt gccgatctta tcgacgccag ttcggaacag gcggcccgtt cggcacttaa
    47221 ctcgctgcaa ggcgcattct ccgcacgggt taatcatctg gtagaagccc tcacccactt
    47281 gcgcatttac gtcgaagcgg caattgattt ccctgatgaa gagatcgatt tcctctccga
    47341 cggcaaaatt gaagcccagc ttaatgacgt gatggccgat ctcgatgcag tgcgtgctga
    47401 agcacgtcag ggtagtttgt tgcgcgaagg gatgaaagta gtgattgccg gacgtcctaa
    47461 tgccggtaaa tcgagcctgt taaacgcgct ggcgggccgt gaagcggcaa tcgtaaccga
    47521 tatcgccgga accacgcgtg acgtgctgcg tgagcatatc cacattgacg gaatgccgct
    47581 gcatatcatc gacactgccg ggctacgtga ggccagtgac gaagtggaac gtattggtat
    47641 cgagcgcgcg tggcaggaaa ttgaacaggc cgaccgcgtg ctgtttatgg tcgatggcac
    47701 cacgacagac gccgttgatc cggcagagat ctggccggaa tttatcgccc gtctgcccgc
    47761 gaaactgccg atcaccgtgg tacgcaataa agccgatatc accggcgaaa cgctgggaat
    47821 gagtgaagtg aacggtcacg cgttaattcg tctctcggca aggactggtg aaggcgtgga
    47881 cgtgctgcgt aaccatctca aacagagcat gggctttgac accaatatgg aaggcggctt
    47941 cctggcgcgc cgtcgccact tacaggcgct ggaacaggca gcggagcatt tgcagcaagg
    48001 taaagcgcaa ctgttgggag cctgggcagg tgaactgctg gcggaagagt tgcgcctggc
    48061 acagcagaac ttaagcgaaa tcaccgggga atttacttca gacgacctgc tggggcggat
    48121 tttctccagc ttctgtattg gtaagtaacc gcgcttacga agccgtattc tgactgtcag
    48181 atgcggcttc gcttcattgt tagcactcct gttatgcctc aacccttttt taaacattaa
    48241 aattcttacg taatttataa tctttaaaaa aagcatttaa tactgctccc cgaacgattg
    48301 tgattcgatt cacatttaaa caatttcaga atagacaaaa attctgagtg taataatgta
    48361 gcggcgtgtc tttcgaggat aagtgcatta tgaatatctt acatgtatgt gtgacctcaa
    48421 aatggttcaa tatcgacaat aagattgtcg atcaccgccc ttaatttacc cttctgtagc
    48481 catcaccaga gccaaaccga ttagattcaa tgtgatcgat tagtttgcta tatcttaatt
    48541 ttgccttttg caaaggccat ctctcgttta tttacttatt ttagtaaatg atggtgcttg
    48601 tatatataca tggtgaatta atggatattg cagatgtaat attcacaggg atcactgtaa
    48661 ttaaaataaa tgaaggatta tgtaatggaa aactttaaac atctccctga accgttccgc
    48721 attcgtgtta ttgagccagt aaaacgtact actcgcgctt atcgtgaaga agcaattatt
    48781 aaatccggta tgaacccgtt cctgctggat agcgaagatg tgtttatcga tttactgacc
    48841 gacagcggca ccggggcggt aacccaaagt atgcaggcag cgatgatgcg cggcgacgaa
    48901 gcctacagcg gcagccgcag ctactatgcg ttagccgagt cagtgaaaaa tatctttggt
    48961 tatcaatata ctattccgac tcaccagggc cgtggcgcag agcaaatcta tattccggta
    49021 ctgattaaaa aacgcgagca ggaaaaaggc ctggatcgca gcaaaatggt ggcattctct
    49081 aactatttct ttgataccac gcagggccat agccagatta acggctgtac cgtgcgtaac
    49141 gtctatatca aagaagcctt cgatacgggc gtgcgttacg actttaaagg caactttgac
    49201 ctcgaaggat tagaacgcgg tattgaagaa gttggcccga ataacgtgcc gtatatcgtt
    49261 gcaaccatca ccagtaactc tgcaggtggt cagccggttt cactggcaaa cttaaaagcg
    49321 atgtacagca tcgcgaagaa atacgatatt ccggtggtaa tggactccgc acgctttgct
    49381 gaaaacgcct atttcatcaa gcagcgtgaa gcagaataca aagactggac catcgagcag
    49441 atcacccgcg aaacctacaa atatgccgat atgctggcga tgtccgccaa gaaagatgcg
    49501 atggtgccga tgggcggctt gctgtgcatg aaagacgaca gcttctttga tgtgtacacc
    49561 gagtgcagaa ccctttgcgt ggtgcaggaa ggcttcccga catatggcgg cctggaaggc
    49621 ggcgcgatgg agcgtctggc ggtaggtctg tatgacggca tgaatctcga ctggctggct
    49681 tatcgtatcg cgcaggtaca gtatctggtc gatggtctgg aagagattgg cgttgtctgc
    49741 cagcaggcgg gcggtcacgc ggcattcgtt gatgccggta aactgctgcc gcatatcccg
    49801 gcagaccagt tcccggcaca ggcgctggcg tgcgagctgt ataaagtcgc cggtatccgt
    49861 gcggtagaaa ttggctcttt cctgttaggc cgcgatccga aaaccggtaa acaactgcca
    49921 tgcccggctg aactgctgcg tttaaccatt ccgcgcgcaa catatactca aacacatatg
    49981 gacttcatta ttgaagcctt taaacatgta aaagagaacg cggcgaatat taaaggatta
    50041 acctttacct acgaaccaaa agtattgcgt cacttcaccg caaaactgaa agaagtttaa
    50101 ttaatactac agagtggcta taaggatgtt agccactctc ttaccctaca tcctcaataa
    50161 caaaaatagc cttcctctaa aggtggcatc atgactgatc aagctgaaaa aaagcactct
    50221 gcattttggg gtgttatggt tatagcaggt acagtaattg gtggaggtat gtttgcttta
    50281 cctgttgatc ttgccggtgc ctggtttttc tggggtgcct ttatccttat cattgcctgg
    50341 ttttcaatgc ttcattccgg gttattgtta ttagaagcaa atttaaatta tcctgtcggc
    50401 tccagtttta acaccatcac caaagattta ataggtaaca cctggaacat tatcagcggt
    50461 attaccgttg ccttcgttct ctatatcctc acttatgcct atatctctgc taatggtgcg
    50521 atcattagtg aaacgatatc aatgaatttg ggctatcacg ctaatccacg tattgtcggg
    50581 atctgcacag ccattttcgt tgccagcgta ttgtggataa gttcgttagc cgccagtcgc
    50641 attacctcat tgttcctcgg gctgaagatt atctcctttg tgatcgtgtt tggttctttc
    50701 ttcttccagg tcgattactc catcctgcgc gatgccacca gcaccactgc gggaacgtct
    50761 tacttcccgt atatctttat ggctttgccg gtgtgtctgg cgtcatttgg tttccacggc
    50821 aatattccca gcctgattat ttgctatggc aaacgcaaag ataagttaat caaaagcgtg
    50881 gtatttggtt cgctgctggc gctggtgatt tatctcttct ggctctattg cacgatgggg
    50941 aatattccgc gcgaaagctt taaggcgata atctcctcag gcggcaacat tgattcgctg
    51001 gtgaaatcgt tcctcggcac caaacagcac ggcattatcg agttttgcct gctggtgttc
    51061 tctaacttag ctgttgccag ttcgttcttt ggtgtcacgc tggggttgtt cgattatctg
    51121 gcggacctgt ttaagattga taactcccac ggcgggcgtt tcaaaaccgt gctgttaacc
    51181 ttcctgccac ctgcgttgtt gtatctgatc ttcccgaacg gctttattta cgggatcggc
    51241 ggtgccgggc tgtgcgccac catctgggcg gtcattattc ccgcagtgct tgcaatcaaa
    51301 gctcgcaaga agtttcccaa tcagatgttc acggtctggg gcggcaatct tattccggcg
    51361 attgtcattc tctttggtat aaccgtgatt ttgtgctggt tcggcaacgt ctttaacgtg
    51421 ttacctaaat ttggctaaat ccttcaagaa gccagccatt cgctggcttc ttgcctctca
    51481 ggaaatcact tatgtccaaa tggcaactcg ccttatcctc cttcaccacg tatgctttgc
    51541 gtcaccttac tatcaggacg ctttagccca tgtcccgctt tttgatttgt agttttgccc
    51601 tggttttact ttatcccgcc gggattgata tgtacctcgt tggtttaccg cgcatcgccg
    51661 ccgatctcaa tgccagcgaa gcgcagttgc atattgcgtt ctccgtatat ctggcgggga
    51721 tggcagctgc gatgctattt gccggtaaag tggccgatcg ttcagggaga aagccggtcg
    51781 ccatacccgg cgcggcgcta tttattattg cctcggtgtt ctgttcactg gctgaaacca
    51841 gcacgttatt tcttgcaggc cgatttctac aggggttggg cgcaggctgt tgttacgtgg
    51901 tggcgttcgc cattttgcgc gacacgctgg atgatcgacg tcgggctaaa gtgctgtcat
    51961 tactcaacgg tattacctgc atcattccgg tgttagcgcc agtgctcgga catctgatta
    52021 tgcttaaatt cccgtggcag agtctgttct gggcgatggc aatgatgggc atcgcggtac
    52081 tgatgttgtc tttgtttatt ttaaaagaaa cgcgcccagc gtcccccgct gcttcggaca
    52141 aaccacgaga aaatagcgag tcgctgctta atcgtttttt cctcagccgt gttgttatca
    52201 ccaccctcag cgtttcggtg atcctcactt tcgtcaatac atcgccggta ttgctgatgg
    52261 aaatcatggg gtttgagcgc ggagaatacg ccaccattat ggcgctgacc gctggcgtca
    52321 gcatgacctt ttcattctcg acgccatttg cgctgggaat ttttaagcca cgtacgttga
    52381 tgatcacctc gcaggtgtta ttccttgcgg cggggatcac ccttgccgtt tcaccttccc
    52441 atgcggtttc tctgtttggt atcacgctga tttgcgccgg tttctcggta ggttttggcg
    52501 tagcgatgag tcaggcgtta ggaccatttt cattacgcgc gggcgtagcc agctcgacct
    52561 taggtattgc gcaggtttgc ggttcgtcac tgtggatttg gctggccgcg gtggttggta
    52621 tcggcgcatg gaatatgctg atcgggattc tgattgcctg tagcatagtg agcctgttgc
    52681 tgattatgtt cgtcgcgcct ggacgccccg ttgccgctca tgaagaaatc catcaccacg
    52741 cttgatctca atctgctgct ctgtctgcaa ctgctgatgc aggagcgcag cgtgaccaaa
    52801 gcggcgaagc ggatgaacgt gaccccttca gcggtgagta agtcgctggc aaagttaaga
    52861 gcgtggtttg acgacccgct ctttgtgaac tcaccgctgg ggctgtcgcc cacacagcta
    52921 atggtcagca tggagcaaaa tctggcggaa tggatgcaaa tgagcaatct gctgctggat
    52981 aaaccgcacc accagacacc gcgcggcctg aagtttgagt tggcggcgga atcgccgctg
    53041 atgatgatca tgcttaatgc gctgtcgaaa cggatctacc aacgttaccc gcaggcgacc
    53101 atcaaactac gtaactggga ttacgattcc ttagatgcca ttactcgtgg tgaagtggat
    53161 attggttttt ccggtcgcga aagccatcct cgctcgcggg agctgttaag ctcgctaccg
    53221 ttagccattg attatgaagt gctgtttagt gatgtaccct gcgtctggtt acgcaaagat
    53281 catccggcac tgcatgaaac gtggaatctg gacacctttt tacgttatcc gcatatcagc
    53341 atttgctggg aacagagcga cacctg
//
LOCUS       DAECOD010000017        53203 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.017, whole genome shotgun sequence.
ACCESSION   DAECOD010000017 DAECOD010000000
VERSION     DAECOD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.017"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(11..322)
                     /locus_tag="KKO15_000995"
     CDS             complement(11..322)
                     /locus_tag="KKO15_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBD6359879.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="KKO15_000996"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KKO15_000996"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="KKO15_000997"
     CDS             701..1054
                     /locus_tag="KKO15_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBD6359880.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KKO15_000998"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KKO15_000998"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBD6359881.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="KKO15_000999"
     CDS             complement(2810..3280)
                     /locus_tag="KKO15_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBD6359882.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KKO15_001000"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KKO15_001000"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBD6359883.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KKO15_001001"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KKO15_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD6359884.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KKO15_001002"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KKO15_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBD6359885.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="KKO15_001003"
     CDS             complement(5283..8432)
                     /locus_tag="KKO15_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD6359886.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KKO15_001004"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KKO15_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBD6359887.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="KKO15_001005"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="KKO15_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBD6359888.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="KKO15_001006"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="KKO15_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBD6359889.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNVMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KKO15_001007"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KKO15_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBD6359890.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KKO15_001008"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KKO15_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBD6359891.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="KKO15_001009"
     CDS             14911..15288
                     /locus_tag="KKO15_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBD6359892.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="KKO15_001010"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="KKO15_001010"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBD6359893.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="KKO15_001011"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="KKO15_001011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBD6359894.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="KKO15_001011"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="KKO15_001012"
     CDS             18099..18428
                     /locus_tag="KKO15_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBD6359895.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="KKO15_001013"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="KKO15_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBD6359896.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="KKO15_001014"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="KKO15_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBD6359897.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="KKO15_001015"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="KKO15_001015"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBD6359898.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="KKO15_001016"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="KKO15_001016"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBD6359899.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KKO15_001017"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KKO15_001017"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBD6359900.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="KKO15_001018"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="KKO15_001018"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBD6359901.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="KKO15_001019"
     CDS             complement(25481..27157)
                     /locus_tag="KKO15_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBD6359902.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KKO15_001020"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KKO15_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBD6359903.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="KKO15_001021"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="KKO15_001021"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD6359904.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KKO15_001022"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KKO15_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBD6359905.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="KKO15_001023"
     CDS             complement(31205..31999)
                     /locus_tag="KKO15_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBD6359906.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="KKO15_001024"
     CDS             32137..32478
                     /locus_tag="KKO15_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD6359907.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KKO15_001025"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KKO15_001025"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBD6359908.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="KKO15_001026"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="KKO15_001026"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBD6359909.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="KKO15_001027"
     CDS             36394..37686
                     /locus_tag="KKO15_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6359910.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALGLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="KKO15_001028"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="KKO15_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBD6359911.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="KKO15_001029"
     CDS             complement(38219..38677)
                     /locus_tag="KKO15_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBD6359912.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="KKO15_001030"
     CDS             complement(38674..39591)
                     /locus_tag="KKO15_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD6359913.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="KKO15_001031"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="KKO15_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBD6359914.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="KKO15_001032"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="KKO15_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBD6359915.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KKO15_001033"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KKO15_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBD6359916.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KKO15_001034"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KKO15_001034"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD6359917.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KKO15_001035"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KKO15_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="KKO15_001036"
     CDS             43550..44236
                     /locus_tag="KKO15_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6359918.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46642
                     /locus_tag="KKO15_001037"
                     /pseudo
     CDS             44233..46642
                     /locus_tag="KKO15_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47354..48447)
                     /gene="mnmH"
                     /locus_tag="KKO15_001038"
                     /pseudo
     CDS             complement(47354..48447)
                     /gene="mnmH"
                     /locus_tag="KKO15_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
     gene            complement(48516..49442)
                     /gene="allS"
                     /locus_tag="KKO15_001039"
     CDS             complement(48516..49442)
                     /gene="allS"
                     /locus_tag="KKO15_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBD6359919.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49672..50154
                     /gene="allA"
                     /locus_tag="KKO15_001040"
     CDS             49672..50154
                     /gene="allA"
                     /locus_tag="KKO15_001040"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBD6359920.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50232..51047
                     /gene="allR"
                     /locus_tag="KKO15_001041"
     CDS             50232..51047
                     /gene="allR"
                     /locus_tag="KKO15_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBD6359921.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51137..52918
                     /gene="gcl"
                     /locus_tag="KKO15_001042"
     CDS             51137..52918
                     /gene="gcl"
                     /locus_tag="KKO15_001042"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBD6359922.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52931..>53203
                     /locus_tag="KKO15_001043"
     CDS             52931..>53203
                     /locus_tag="KKO15_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBD6359923.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12825 a        13689 c        14016 g        12673 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgtgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggatactttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaatgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatccct aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct gggtttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagta caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtag cgatgaatca ttactgtcgc agtcgctacg atctggtggc gatcctcaaa
    45061 acgctggggg cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg
    45121 ctgacgcttt cagccgttac cggtggggcc ataggcctgt tgttcgaaaa cgtgttgatg
    45181 gtgctgctca agcccgttct gcctgctgca ctaccgccag ccagcctctg gccgtggctg
    45241 tgggcgcttg gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg
    45301 ttgctggcaa cgcagccttt acgcgtatta cgtaatgatg tggtcgcgaa cgtctggccg
    45361 ctgaagtttt atctgccgat tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg
    45421 ggtggcagca tgctgctttg ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc
    45481 ggtgtgctgg gctggatgct gctgaatgta cttcgccgca tgacgctgaa atcgctgcct
    45541 ctgcgcctgg cggttagccg cctgttacgt cagccgtggt caacgttaag ccagctttcg
    45601 gcattttcgc tctcctttat gctgctggca ctgctgctgg tgctgcgtgg cgatctgctc
    45661 gaccgctggc agcagcagct accgccagaa agcccgaact actttttaat taacatcgcc
    45721 acagaacagg tagcaccgct aaaagcgttc ctcgcggaac atcatatagt cccggaatca
    45781 ttttatccgg tagtgcgggc gcggctgacg gcgattaatg ataagccgac agaaggtaat
    45841 gaagatgagg cgcttaatcg cgaactcaat cttacctggc aaaatacgcg gcccgatcat
    45901 aatccgattg tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat ggaagagggg
    45961 ctggcgaaac gcttaaacgt tgccctcggt gataccgtga cttttatggg cgatacccag
    46021 gagttccgcg ctaaagtgac cagcctgcgc aaagtggact gggaaagtct gcggcctaat
    46081 ttctatttta ttttccctga aggggcatta gacgggcaac cgcagagctg gcttaccagt
    46141 ttccgctggg agaatggcaa cggcatgttg acgcaactca accgccagtt cccgaccatt
    46201 agcctgttag acattggcgc gattttaaaa caggtcggtc aggtgctgga gcaggtaagt
    46261 cgggcgctgg aggtgatggt ggtgctggtc accgcctgcg gtatgttgct gttgctggca
    46321 caggtgcagg tgggaatgcg ccagcgtcat caggagctgg tggtatggcg cacactcggt
    46381 gcggggaaaa aactgctgcg cacgacattg tggtgtgatg ttcgccatgc ttgggtttgt
    46441 ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt
    46501 gtttgatttc ccgtgggagc cagactggcg attgtggatt gttctgccgt gcagcggtgc
    46561 gctgctgctg tcgcttttcg gcggctggct gggtgcgcga ctggttaagg gtaaggcgct
    46621 gttcaggcag tttgtggggt gatgacaggt gttagattgc ctgatgcgct acgcttatca
    46681 ggcctacgaa gagcgtgcaa tatattgatt ttgcaagatt gtgtaggccg gataaggcgt
    46741 ttacgccgca tctggcatga acagtgtgca ttttggcaat aaatatgaaa ccaactgtca
    46801 tgtcggtttt tttaaaggcc gaaggaggta aaccttcggc cttagctacg tttcctcagt
    46861 tattcaataa actgacggat cagttccagc agcgcgatta gcgccttaag aacgatgaga
    46921 actttatcga gaaaagtttt catccgttgc tcccttaagg gagaaagaca atcacagctt
    46981 acccttacgt tgcctataac tatgattttc ttcatgtgct agatgaggac tgtctctctc
    47041 caaattagag caccagaatc agcaccacct gtatcctgac caggacttaa aagtactttt
    47101 ccgcactgcg gtaatccctt gaggcagtat cactactagc gtcgggattg tatcgccaac
    47161 atagctgttt gaatatgtag gcatcctgac ttataattca cttgctagat gaggactgtt
    47221 taatttctgc ccggaaccac caagtcttgg gcggaaaaaa taaaaaaacc gactgtcatg
    47281 tcggtttttt atatctgtat tctggtgcaa ccattttgtt ctcacgacaa tcgccgtttc
    47341 agaataccgc ccatcactgc gccttaaccc ataccgccac ttccgcccac tcaccgcgaa
    47401 agacaacttt ttccgctttt ttctcaagct gatagcgata cattgggtcg taatattctt
    47461 taagtaacgg caccagccag gccagatgac cgtcggtgct gccggtgtga gttgcgttgt
    47521 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    47581 aatcgccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    47641 ataagcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcattca ggcgttcaag
    47701 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    47761 acattccggc aggtgattcg aaccgatcat ccggctttca tcttccagca cccacaggcg
    47821 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    47881 ctggctaagt tgtggttgta acgtgcgacc aaacgccgaa ccgcgatgac gcgccagccc
    47941 ttccagatca acaccgtttg gctgttgttg cactaatagc gttttaccgc tgccggtaca
    48001 accgccaatc agcactatcg gtttttgtgc cagttcaatg gtcgcctgaa tcgcggtctg
    48061 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctt catgcagcca
    48121 gcgttgcaca atatgtgaac gttgaccgcc acgggcgcag cagagaatac cttgcggatt
    48181 ttgcaggcac gctgcccgcc aggcgtacat acgctgctga cgaatttcac ccgccaccag
    48241 tttatgcccc agcgccagcg ctgcgtctga gccttgctgt ttatagcagg tgccaacgga
    48301 ggcgcgttca tcgttatcca ttaacggcag attgatagcg gcgggcattg cgccttgctc
    48361 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    48421 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    48481 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtggggt gccgaaaatt
    48541 tctaaaaatc cgctgatttc cggcctgtgt tgggtaaaca aggtcacaat atcttctacc
    48601 gctttgcagc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    48661 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    48721 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    48781 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt gccggcgctg
    48841 tcttcaatat tgaccgccgg aaagcgacgc aactgcgctt ctgttagcgg ctcttcaacg
    48901 ttcgccagcg gatgatccgc cgccatgaca aatcgccatt gcaccgatcc taagggatca
    48961 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    49021 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    49081 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    49141 aggttgttga tgacaatatt cacctggcgt tccacgccat cattcacctg ttgcagctcg
    49201 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    49261 gccgctgtca acgttacgct acgagtcgta cggaaaaaca gcgctactcc ggtattctct
    49321 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    49381 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    49441 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    49501 cagtattttt acatgataat cctgttcaat ttgtggacta aatctagttt tggaaaaaca
    49561 ttccaacttt tgtattgatg ctgttctctt aaggttttag attgcctgtt attgaaacca
    49621 agctgaccgg tcggcggtgg ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt
    49681 caggtattac cgttaagtca ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag
    49741 caacgggatt ttttccatat taacaatggc ctggtggagc gttaccacga tttggcgctg
    49801 gttgagattc ttgagcaaga ccgtacgctt atcagcatta accgcgcgca accggcgaat
    49861 ctgccgctga ccattcacga actcgaacgt catccgctgg gcactcaggc ctttatccca
    49921 atgaaaggtg aagtgtttgt ggtggtcgtg gcgttaggcg acgacaaacc agacctgtca
    49981 acgctgcggg cgtttatcac caacggtgaa cagggggtga attaccatcg taacgtctgg
    50041 catcacccac ttttcgcctg gcagcgcgtc accgattttc tgaccatcga tcgcggcggc
    50101 agtgacaact gtgatgttga aagtattcct gaacaggaac tctgttttgc gtgacgcctg
    50161 caaccgactt gcataagata aactaattgt tcattgttta tgctcacttg taggtcggag
    50221 ttaacgtagg tatgacggaa gttagacggc gcggcaggcc aggacaggcg gagcctgtgg
    50281 cacagaaggg cgcacaggcg ttagagcggg gaattgcgat tctgcaatat ctggaaaaaa
    50341 gtgggggaag ttcgtcggtt agcgatattt ctctcaatct ggatttgccg ctctccacga
    50401 cctttcgctt gctgaaggtt ttacaagcag cggattttgt ctatcaggac agtcaattag
    50461 gctggtggca tataggatta ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg
    50521 atgtcctctc cgtcgccggg ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg
    50581 tcaatgtcgc gatccgtaac ggcaatgaag cggtattaat tggtcagtta gagtgtaaat
    50641 cgatggtcag gatgtgtgcg ccactgggca gtcgtctgcc gctgcatgct tccggtgcgg
    50701 gcaaagcgct gctttatccg ctggcggaag aggagttgat gagcatcatt ctgcaaaccg
    50761 gtttgcagca gtttacgcca actacgcttg tggatatgcc caccttgctg aaggacctgg
    50821 aacaagcgcg tgaactgggc tataccgtag ataaagaaga gcatgttgta ggtctgaatt
    50881 gcatagcttc agcaatttac gatgatgtcg gtagagttgt tgccgctatc tccatctccg
    50941 ggccttcatc aagactgaca gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca
    51001 ccgcccgcga tatcagcacg gcgttgggac tgaaagcaca tccatagtgt ctgtcgcatc
    51061 ccgctctgcg gagcgggttt ttttgacaaa atttgaaagt tggaaaattt ttccaattaa
    51121 tagaggtagg aataaaatgg caaaaatgag agccgttgac gcggcaatgt atgtgctgga
    51181 gaaagaaggt atcaccaccg ccttcggcgt tccgggagct gcaattaatc cgttctactc
    51241 agcgatgcgt aagcacggcg gtattcgtca cattctggcg cgtcatgtgg aaggtgtttc
    51301 gcacatggcg gaaggttata cccgcgcaac ggcagggaat atcggcgtat gtctggggac
    51361 ttccggtcct gcgggcacgg acatgatcac cgcgctctat tccgcttctg ctgattccat
    51421 tcctattctg tgcattaccg gccaggcacc gcgcgcccgt ctgcataaag aagattttca
    51481 ggccgtagat attgaagcaa ttgctaaacc ggtcagcaaa atggcggtta cagttcgtga
    51541 agcggcgctg gtgcctcgcg tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc
    51601 gggtccggta ctggtggatt taccgttcga cgttcaggtt gcggaaatcg agtttgatcc
    51661 tgacatgtac gaaccgctgc cggtctacaa acctgctgcc agccgtatgc agatcgaaaa
    51721 agctgtagaa atgttaatcc aggccgaacg tccggtgatt gttgccgggg gcggggtaat
    51781 caatgctgac gcagctgcac tgttacaaca gtttgctgaa ctgaccagcg ttccggtgat
    51841 cccaacgctg atgggctggg gctgtatccc ggacgatcat gaactgatgg ccgggatggt
    51901 cggtctgcaa accgcgcatc gttacggtaa cgcaacgttg ctggcgtccg acatggtgtt
    51961 tggtatcggt aaccgttttg ctaaccgtca taccggttcg gtagagaaat acaccgaagg
    52021 gcgcaaaatc gttcatattg atattgagcc gacgcaaatt ggtcgcgtgc tgtgtccgga
    52081 tctcggcatt gtctctgatg ctaaagcggc gctgacactg ctggttgaag tggcgcagga
    52141 gatgcaaaaa gcgggtcgtc tgccgtgtcg taaagaatgg gtcgccgact gccagcagcg
    52201 taaacgcact ttgctgcgca aaacccactt cgacaacgtg ccggtgaaac cgcagcgcgt
    52261 gtatgaagag atgaacaaag cctttggtcg cgatgtttgt tatgtcacca ctattggtct
    52321 gtcacaaatc gctgcggcac aaatgctgca tgtctttaaa gaccgccact ggatcaactg
    52381 tggtcaggct ggtccgttag gctggacgat tccggcggcg ctgggggttt gtgccgctga
    52441 tccgaaacgc aatgtggtgg cgatttctgg cgactttgac ttccagttcc tgattgaaga
    52501 gttagccgtt ggcgcgcagt tcaacattcc gtacatccat gtgctggtga ataacgccta
    52561 tctcggcctg attcgtcagt cacaacgcgc ttttgacatg gactactgcg tgcaactcgc
    52621 tttcgagaat atcaactcca gtgaagtgaa tggctacggt gttgaccacg taaaagtagc
    52681 ggaaggttta ggttgtaaag ctattcgggt cttcaaaccg gaagatattg cgccagcctt
    52741 tgaacaggcg aaagccttaa tggcgcaata tcgggtaccg gtagtcgtgg aagttattct
    52801 cgagcgtgtg accaatattt cgatgggcag cgaactggat aacgtcatgg aatttgaaga
    52861 tatcgccgat aacgcagcgg atgcaccgac tgaaacctgc ttcatgcact atgaataagg
    52921 gagataaatg atgttacgtt tctctgctaa tttatcgatg ttatttggtg aatatgattt
    52981 tctcgcccgt tttgagaaag ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc
    53041 ttatgactac gacattgaag aattaaaaca ggtactggcg agtaataaac tcgaacatac
    53101 gctgcacaat ttaccggcag gtgactgggc ggcgggcgag cgcggtattg cctgtattcc
    53161 tggccgtgaa gaagagtttc gggatggcgt agcagcagcg att
//
LOCUS       DAECOD010000018        49831 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.018, whole genome shotgun sequence.
ACCESSION   DAECOD010000018 DAECOD010000000
VERSION     DAECOD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.018"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1225
                     /locus_tag="KKO15_001044"
     CDS             <1..1225
                     /locus_tag="KKO15_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6359924.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1222..2187
                     /gene="yggF"
                     /locus_tag="KKO15_001045"
     CDS             1222..2187
                     /gene="yggF"
                     /locus_tag="KKO15_001045"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD6359925.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2209..2718
                     /gene="fumE"
                     /locus_tag="KKO15_001046"
     CDS             2209..2718
                     /gene="fumE"
                     /locus_tag="KKO15_001046"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBD6359926.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2715..3428
                     /locus_tag="KKO15_001047"
     CDS             2715..3428
                     /locus_tag="KKO15_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBD6359927.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3400..4077)
                     /locus_tag="KKO15_001048"
     CDS             complement(3400..4077)
                     /locus_tag="KKO15_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD6359928.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4071..4748)
                     /locus_tag="KKO15_001049"
     CDS             complement(4071..4748)
                     /locus_tag="KKO15_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBD6359929.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4736..5119)
                     /locus_tag="KKO15_001050"
     CDS             complement(4736..5119)
                     /locus_tag="KKO15_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBD6359930.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5135..5443)
                     /locus_tag="KKO15_001051"
     CDS             complement(5135..5443)
                     /locus_tag="KKO15_001051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359931.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5444..6022)
                     /locus_tag="KKO15_001052"
     CDS             complement(5444..6022)
                     /locus_tag="KKO15_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBD6359932.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6045..6476)
                     /locus_tag="KKO15_001053"
                     /pseudo
     CDS             complement(6045..6476)
                     /locus_tag="KKO15_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6848..7867
                     /gene="epd"
                     /locus_tag="KKO15_001054"
     CDS             6848..7867
                     /gene="epd"
                     /locus_tag="KKO15_001054"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBD6359933.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLFLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7917..9080
                     /gene="pgk"
                     /locus_tag="KKO15_001055"
     CDS             7917..9080
                     /gene="pgk"
                     /locus_tag="KKO15_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBD6359934.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9295..10374
                     /gene="fbaA"
                     /locus_tag="KKO15_001056"
     CDS             9295..10374
                     /gene="fbaA"
                     /locus_tag="KKO15_001056"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBD6359935.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10565..11425
                     /gene="mscS"
                     /locus_tag="KKO15_001057"
     CDS             10565..11425
                     /gene="mscS"
                     /locus_tag="KKO15_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBD6359936.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11564..12199
                     /gene="argO"
                     /locus_tag="KKO15_001058"
     CDS             11564..12199
                     /gene="argO"
                     /locus_tag="KKO15_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBD6359937.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12292..13032
                     /locus_tag="KKO15_001059"
     CDS             12292..13032
                     /locus_tag="KKO15_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBD6359938.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13199..14149
                     /locus_tag="KKO15_001060"
     CDS             13199..14149
                     /locus_tag="KKO15_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6359939.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14091..15569)
                     /gene="scpC"
                     /locus_tag="KKO15_001061"
     CDS             complement(14091..15569)
                     /gene="scpC"
                     /locus_tag="KKO15_001061"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBD6359940.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15593..16378)
                     /gene="scpB"
                     /locus_tag="KKO15_001062"
     CDS             complement(15593..16378)
                     /gene="scpB"
                     /locus_tag="KKO15_001062"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBD6359941.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16389..17384)
                     /gene="meaB"
                     /locus_tag="KKO15_001063"
     CDS             complement(16389..17384)
                     /gene="meaB"
                     /locus_tag="KKO15_001063"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBD6359942.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17377..19521)
                     /gene="scpA"
                     /locus_tag="KKO15_001064"
     CDS             complement(17377..19521)
                     /gene="scpA"
                     /locus_tag="KKO15_001064"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBD6359943.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19725..20618)
                     /gene="argP"
                     /locus_tag="KKO15_001065"
     CDS             complement(19725..20618)
                     /gene="argP"
                     /locus_tag="KKO15_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBD6359944.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21001..21660
                     /gene="rpiA"
                     /locus_tag="KKO15_001066"
     CDS             21001..21660
                     /gene="rpiA"
                     /locus_tag="KKO15_001066"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBD6359945.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21916..23148
                     /gene="serA"
                     /locus_tag="KKO15_001067"
     CDS             21916..23148
                     /gene="serA"
                     /locus_tag="KKO15_001067"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBD6359946.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23377..23436
                     /gene="ibsC"
                     /locus_tag="KKO15_001068"
     CDS             23377..23436
                     /gene="ibsC"
                     /locus_tag="KKO15_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBD6359947.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23769..23828
                     /locus_tag="KKO15_001069"
     CDS             23769..23828
                     /locus_tag="KKO15_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD6359948.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23929..24531)
                     /locus_tag="KKO15_001070"
     CDS             complement(23929..24531)
                     /locus_tag="KKO15_001070"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBD6359949.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24552..24735)
                     /gene="ssrS"
                     /locus_tag="KKO15_001071"
     ncRNA           complement(24552..24735)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KKO15_001071"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24777..25106)
                     /gene="zapA"
                     /locus_tag="KKO15_001072"
     CDS             complement(24777..25106)
                     /gene="zapA"
                     /locus_tag="KKO15_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBD6359950.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25274..25852
                     /gene="ygfB"
                     /locus_tag="KKO15_001073"
     CDS             25274..25852
                     /gene="ygfB"
                     /locus_tag="KKO15_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBD6359951.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGKGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25878..27203
                     /gene="pepP"
                     /locus_tag="KKO15_001074"
     CDS             25878..27203
                     /gene="pepP"
                     /locus_tag="KKO15_001074"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBD6359952.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27200..28378
                     /gene="ubiH"
                     /locus_tag="KKO15_001075"
                     /gene_synonym="visB"
     CDS             27200..28378
                     /gene="ubiH"
                     /locus_tag="KKO15_001075"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBD6359953.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28401..29603
                     /gene="ubiI"
                     /locus_tag="KKO15_001076"
                     /gene_synonym="visC"
     CDS             28401..29603
                     /gene="ubiI"
                     /locus_tag="KKO15_001076"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBD6359954.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29677..29760)
                     /locus_tag="KKO15_001077"
     CDS             complement(29677..29760)
                     /locus_tag="KKO15_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359955.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30051..31145
                     /gene="gcvT"
                     /locus_tag="KKO15_001078"
     CDS             30051..31145
                     /gene="gcvT"
                     /locus_tag="KKO15_001078"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBD6359956.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTWEFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31169..31558
                     /gene="gcvH"
                     /locus_tag="KKO15_001079"
     CDS             31169..31558
                     /gene="gcvH"
                     /locus_tag="KKO15_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBD6359957.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31677..34550
                     /gene="gcvP"
                     /locus_tag="KKO15_001080"
     CDS             31677..34550
                     /gene="gcvP"
                     /locus_tag="KKO15_001080"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBD6359958.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVVHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34817..35560
                     /locus_tag="KKO15_001081"
     CDS             34817..35560
                     /locus_tag="KKO15_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6359959.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35617..37050)
                     /gene="bglA"
                     /locus_tag="KKO15_001082"
     CDS             complement(35617..37050)
                     /gene="bglA"
                     /locus_tag="KKO15_001082"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBD6359960.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37095..37405
                     /locus_tag="KKO15_001083"
                     /pseudo
     CDS             37095..37405
                     /locus_tag="KKO15_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37569..38228
                     /locus_tag="KKO15_001084"
     CDS             37569..38228
                     /locus_tag="KKO15_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBD6359961.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38424..39404)
                     /gene="ygfZ"
                     /locus_tag="KKO15_001085"
     CDS             complement(38424..39404)
                     /gene="ygfZ"
                     /locus_tag="KKO15_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBD6359962.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39647..39913
                     /gene="sdhE"
                     /locus_tag="KKO15_001086"
     CDS             39647..39913
                     /gene="sdhE"
                     /locus_tag="KKO15_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBD6359963.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39894..40301
                     /locus_tag="KKO15_001087"
     CDS             39894..40301
                     /locus_tag="KKO15_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBD6359964.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40341..40862)
                     /gene="fldB"
                     /locus_tag="KKO15_001088"
     CDS             complement(40341..40862)
                     /gene="fldB"
                     /locus_tag="KKO15_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBD6359965.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40974..41870
                     /gene="xerD"
                     /locus_tag="KKO15_001089"
     CDS             40974..41870
                     /gene="xerD"
                     /locus_tag="KKO15_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBD6359966.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41895..42605
                     /gene="dsbC"
                     /locus_tag="KKO15_001090"
     CDS             41895..42605
                     /gene="dsbC"
                     /locus_tag="KKO15_001090"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBD6359967.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42611..44344
                     /gene="recJ"
                     /locus_tag="KKO15_001091"
     CDS             42611..44344
                     /gene="recJ"
                     /locus_tag="KKO15_001091"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBD6359968.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44435..45533
                     /gene="prfB"
                     /locus_tag="KKO15_001092"
     CDS             join(44435..44509,44511..45533)
                     /gene="prfB"
                     /locus_tag="KKO15_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBD6359969.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45543..47060
                     /gene="lysS"
                     /locus_tag="KKO15_001093"
     CDS             45543..47060
                     /gene="lysS"
                     /locus_tag="KKO15_001093"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD6359970.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47103..47651)
                     /gene="idi"
                     /locus_tag="KKO15_001094"
     CDS             complement(47103..47651)
                     /gene="idi"
                     /locus_tag="KKO15_001094"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBD6359971.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47706..47777
                     /locus_tag="KKO15_001095"
     CDS             47706..47777
                     /locus_tag="KKO15_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359972.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47774..47899)
                     /locus_tag="KKO15_001096"
     CDS             complement(47774..47899)
                     /locus_tag="KKO15_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359973.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47901..49349)
                     /gene="uacT"
                     /locus_tag="KKO15_001097"
     CDS             complement(47901..49349)
                     /gene="uacT"
                     /locus_tag="KKO15_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD6359974.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12052 a        12455 c        13403 g        11921 t
ORIGIN      
        1 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
       61 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
      121 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
      181 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
      241 gttaccaagc ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta
      301 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
      361 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa
      421 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
      481 tgcactgctg gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg
      541 tggcatacaa ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt
      601 gcagaagctg ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa
      661 taccaaaggg atgagcgatc ctatccagat gctgcgggcg ctgacaggag atgtcgggtt
      721 cgatgacatt tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact
      781 ggcggaagat ggctgtctga acttctttgc cgggctgacg gataaaaact tcaaagtgcc
      841 gtttaatttc tacaacgtcc attacaacag cacgcacgta gtcggtacat ccggcggttc
      901 aacggacgac atgaaagagg cgattgccct tagcgccaca ggacagttac agccgtcgtt
      961 tatggtgacc catattggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga
     1021 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     1081 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
     1141 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     1201 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     1261 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     1321 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     1381 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     1441 ggcgatgggc cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg
     1501 atggggcaga gcaatgcgct ggcggtcatg gctttcgctc ctcgagatag cctgttacat
     1561 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     1621 ctgtctttgc ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taaaccgctg
     1681 gacaagctgc gcatggttac gctggataaa ccgcgtctga gtgctgcgat tgaagaagca
     1741 acacagctgg gcgtgaaggt ttttgccctg ccggatggcg atgtcgccgc cagcgtgctg
     1801 acctgctggc aggataatcc ttacgatgtg atgtacacca tcggcggtgc gccagaaggc
     1861 gtgatttctg cctgtgccgt taaagcgtta ggcggggata tgcaggccga gctgattgac
     1921 ttttgtcagg ctaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
     1981 cgttgtaagg caatgggcgt tgacgttaac cgtgtgtact cgctcgatga actggtgagg
     2041 gggaacgata tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc
     2101 caacagacgg cgaatggggt gcggacgcag acattactga tcggcggcgc ggaccaaacg
     2161 tgtaatataa tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg
     2221 acagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
     2281 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     2341 aacgatgaag agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     2401 gacgatattg atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     2461 tatgccgata tcacgcactt ttcccagtac tggcattacc tgaacgaaca ggatgaaacg
     2521 cccggatttg ccgatgatat aacctgggat tttatcagca atgtcaacag tataactcgc
     2581 aatgcgacgc tctacgatgc gttaaaagcg atgaagttcg ccgatttcgc cgtctggtca
     2641 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
     2701 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
     2761 accatgatga agagatagaa cgcgtgcata aactgttgct gcgtatgctg gcggccttac
     2821 agacggtaaa tccgcagcgg cgtacggtgg tttttctttg cgctccgccg gggacgggta
     2881 aatctaccct aaccaccttc tgggaatatc tcgcgcaaca agacccggag ctgcccgcta
     2941 ttcaggcgct cccgatggat ggttttcacc attacaatag ctggctggac gcgcatcaat
     3001 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgc
     3061 gccaggtggt ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg
     3121 ttgaagatgc gttgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc
     3181 tggacgacga gaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaacg
     3241 cgccagctgc tgcgttgcgg gagcgtctgg tggggcgcaa gctggcggga ggggtatcgc
     3301 tggcagatgc agaagccttt tatgaccgta ccgacgggcc gaatgttcgc cgggtgctgg
     3361 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
     3421 tggattaacg ttcctgatta caccatcttc cagccgcacc acacgagaga aatgacgccg
     3481 ggtaaatgcg gcgtcatgac tgattgctac cacgctcgtg cctcgctggc ggcacttctc
     3541 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
     3601 cagtaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
     3661 actatgcaga tccaacgggt gcgagttggc agtgtctgct aactgacaac attgcagggc
     3721 agcatgggta cgttgagtga tttcatccgc cgggcatttt tgcagtttca aaccaaatgc
     3781 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
     3841 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3901 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     3961 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4021 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4081 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4141 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4201 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     4261 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4321 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4381 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4441 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4501 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4561 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4621 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4681 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4741 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4801 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4861 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4921 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     4981 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5041 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5101 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5161 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5221 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5281 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5341 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5401 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5461 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5521 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5581 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5641 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5701 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5761 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5821 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5881 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     5941 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6001 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6061 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6121 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6181 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6241 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6301 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6361 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6421 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6481 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6541 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6601 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6661 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6721 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6781 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6841 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6901 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     6961 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7021 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7081 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7141 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7201 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7261 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7321 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7381 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7441 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7501 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7561 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7621 tgaagtcaac ctgtttctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7681 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7741 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7801 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7861 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7921 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     7981 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8041 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8101 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8161 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8221 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8281 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8341 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8401 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8461 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8521 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8581 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8641 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8701 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8761 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8821 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8881 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     8941 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9001 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9061 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9121 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9181 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
     9241 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9301 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9361 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9421 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9481 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9541 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9601 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9661 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9721 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9781 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9841 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9901 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
     9961 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10021 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10081 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10141 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10201 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10261 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10321 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10381 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10441 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10501 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10561 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10621 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10681 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10741 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10801 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10861 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10921 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    10981 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11041 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11101 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11161 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11221 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11281 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11341 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11401 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11461 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11521 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11581 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11641 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11701 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11761 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11821 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11881 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    11941 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12001 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12061 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12121 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12181 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12241 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12301 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12361 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12421 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12481 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12541 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12601 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12661 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12721 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12781 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12841 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12901 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    12961 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13021 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13081 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13141 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13201 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13261 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13321 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13381 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13441 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13501 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13561 gaccgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13621 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13681 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13741 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13801 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13861 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13921 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    13981 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14041 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14101 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14161 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14221 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14281 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14341 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14401 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14461 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14521 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14581 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14641 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14701 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14761 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14821 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14881 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    14941 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15001 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15061 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15121 caggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15181 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15241 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15301 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15361 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15421 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15481 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15541 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15601 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15661 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15721 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15781 cagagatgtg gtgcgccatt tgtaaggtga aatcttccag ttcttccact tccacaacat
    15841 ggttgaggat gccgacagcc agcgcgcgct gggcggtgat tggcgaagcg gtaaaaatca
    15901 gctctttgac aatgtggaag cccgcgtcgc gggtcaggtt gtgaatgccg accaggttat
    15961 acgggacgcc gaggtttaca ggcgtcattg agaaggttga ggtactggcg gcgatgatca
    16021 gatcggaact catgatcatt tcaaatgcgc caccccaaac actaccttcc accatcgaaa
    16081 tgatcggttt cgggaatttt tggatcatgc gggtgatttg acgcaatgga tcatcatagg
    16141 agagcggatc gcgaccgcca gacggcagtt cgtgaatatc gtgacctgcg gagaagactt
    16201 tggatccact tggcgcccgc aaaatgatac agcgaatttc cggccggttg agatcgctta
    16261 acgcctgcat aagatcatca ataaagactt tacttaaggc attaagtttt cgtccatagt
    16321 taaactcaat gaccgccact ttgttgatag tggcaacgtt cacatactga taagacataa
    16381 aaattccttt aatcaaaata ttgcgtctgg ataaattcac tgagctgccg caggccggtg
    16441 cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct ggcggtaata gcgatcgaaa
    16501 tcttcattcg cgaacaggtg attcagtact tcttcttcgg tctgcttacg cagccattcc
    16561 accgattgtt gttgccgcac ttgttgtaaa cgaccactgg cagttagcgc ggttttgaag
    16621 tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt ccagtgcgct acaagtcaga
    16681 ccccgtggct gccattcgtc gtatttacgt cgcagaatat gcagggcact ctcgtacata
    16741 tgccgggcaa tggcgacatt ggtatggtta tcgccatcgt ctttgttgat aacgagcaga
    16801 tcagccactt ccatcagccc ttttttaatg ccctgcagat catcgccgcc accggcaatt
    16861 tgcaacgaga taaaacagtc caccatgcgg gcgacttccg tttccgactg cgcgacgcca
    16921 accgtttcga caatcactac gtcataaccc gccgcttcgc ataacagcat taattcccgt
    16981 gctcgctgac tggcaccgcc cagatgaccg gaggagggta ccgggcgaat aaacgccgcc
    17041 tcggcacgcg ccaggtcatt catgcgggtt ttatccccga gaatgctacc gccagtgacc
    17101 gggctgctgg gatcgaccgc aataacagca acctttaatc cctcacgaat caacaacatg
    17161 ccaaaggctt caagaaaggt acttttcccc gcgccgggtg tgccggtaac gcccagtcgc
    17221 agggcgttac cacagaacgg cataatagcg tcaagcagct gtgtacttag cgcctgatga
    17281 cgtgggtgac ggctttccac cagcgtcatg gcttgggcga gcgtggcacg ctcaccctga
    17341 cgtaagcggc gaatactttc tgccagcgtg gcttcattaa tcatgatgct ggcttatcag
    17401 attcagtacg tcgcgcacac tgtcgagcat aggtgtacct ggaccataaa tcgccgccac
    17461 gccgcgctct tgcaggaagg cgtaatcctg cggcggaatg acgccacccg cgaccacgca
    17521 gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt tccgggatca gcgttttatg
    17581 accggcagcc agtgaggatg cgcccactac gtgaacgtcg ttttctacgg ccaggcgggc
    17641 gatctcttca ggtgtagaga acatcgggct taaatctacg tcgaaaccga gatcggaata
    17701 ggcgctggcg atcactttcg cgccgcgatc gtgtccatcc tggcccatct tagcgatcag
    17761 aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc gtttgcgcaa caatggcatc
    17821 gaactcggag gccgatttct cagactgatg atagctttgc gcaatcacgc cggtaacaca
    17881 ctggcttggc accagataac ggtcgaaagc gacttccagc gcatcggaaa tttcacccag
    17941 ggtggcgcga acgcgagcgg cattaacagc ggcagccagc aggttttcgt tatgctgtgc
    18001 ggcgtgagtc agggcgttca acgcggcggt tacggcggca tcatcacggg tggcgcgaat
    18061 gcgttccagc gaagcaattt gctcgttacg caccatcacg ttgtcgatct caagtacatc
    18121 ggtttcgtct tcgtgatcca gtttgtactt gttgacacca acgatgacac gcttgccctg
    18181 gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc attcgttttg gcagacctgc
    18241 ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt tggataatag ctctggcttg
    18301 tttgacgatt tgatcggtca gcgactcaat gtaataggat ccggccagtg gatcgacggt
    18361 gtggcagagt tctgattctt cctggatgat gatctgggtg ttgcgggcaa tgcgtgctga
    18421 gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg gtatgcagtg actgagtacc
    18481 gcccagcgtc gcagccagtg cttcaatggt ggtgcggata acgttgttat acggatcctg
    18541 ttcagtcagg ctccagcctg aggtctggca gtgggtacgc agcgccagtg atttcggatc
    18601 ctgtgcgcca aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc
    18661 gacgttcata aacagatcca tgccgatacc gaagaataac gacaggcgag gagcgaagtc
    18721 atcaattttc agtccggcag agattgctgc tttgatgtac tcaatcccat cggcgagcgt
    18781 aaatgctacc tgctgcacgc agttggcacc cgcttccccc atgtggtaac cgctgatact
    18841 gatggtatta aatcgcggca tgttgccgga acaccaggcg atgatgtcgg cgataatgcg
    18901 cattgacggt tttggtgggt aaatataggt gttgcggcag aggtactctt tgagaatatc
    18961 gttttgaatg gtgccggtca gtttatcagg tgtaacacct tgctcttctg cggcgacgat
    19021 ataaaacgcc agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc
    19081 cagcgggatc tggtcgaaca ggactttcat atcttccacg gtgtcgatag cgacgcccgc
    19141 tttgccgacg tcacccgcca cgcgtgggtt atcggagtcg tagccacggt gggtggcaag
    19201 gtcaaacgca acggaaagac ctttttgccc ggcggccagg ttacggcgat aaaaagcgtt
    19261 ggactctttt gctgttgaaa aaccagcata ctgacggatg gtccacggtt gggcggtata
    19321 catagtggca cgcgggccac gaacgtaggg cggcaaacca ggaagggtac ctgtcacctc
    19381 cagattatcg agatcggctt cggtatacag cggcttgatg gcgatccctt ccgcggtttg
    19441 ctgaaccagc gagtcgacag ttttctcccg acggctcaat tccttgttgg caagctgttg
    19501 ccactcctgc tcgttagaca ttatttgctc cgtaaaacgc aatcaaaatg agggtatttg
    19561 cgcttacggt gagcttttta cgtgggtttg tcgccagcaa aaaaaatgca ttcagacgtc
    19621 gttttttgcc gcctgagatt tgtctatttt gtgatgtgat tcaaggaata caaaaagaag
    19681 tgaggacaac gcctgatatg tgctatcagg cattatttga tggattaatc ctgacgaagg
    19741 actttgtgac cataatcgag taacgcatca gtgactttac gcatcatgcg gctttcagga
    19801 gcaaagcggt gccagtagag catccgtcgt tgaaatagcc caggcgttaa gtcaatcagt
    19861 tcaccactgg ccagctcttt ctcgatttgc aggtgcggga tcatacagca ggtggtgccc
    19921 tggcgagcaa gttgtacgaa cgcttctgaa gaattaacga tatggcaggg tacgctgcca
    19981 ggaggaagat cgaagttttg ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac
    20041 gcgaccactg gcgctttcaa taatgccgaa cgcgttacgc cgttaggaaa atatttttcg
    20101 gcaaacggtt ttgagctgac gaacagatag tcgagcgcac cgagtttatc gacaagacaa
    20161 ctcggcagcg cctgatgttg aatactcacc gcgccgacca cttcgccgcg gcgcagacgt
    20221 tcctgagtac gggtttcatc ttctacctgc aagttgaggc ggataggcga atcagccaac
    20281 acaggagcca gtgccggaag caaccacgtc gccagactgt cggcgttgac cgccagtgaa
    20341 agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc actcttcttc cagcaactcc
    20401 acctggcgca gcagtgccag cagtttttgc ccttgttccg tcgggcgcgg cggtacggta
    20461 cgcaccaaca gcggctgccc gaacatattt tccagttgct taatgcgctg tgagacggct
    20521 gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca
    20581 tccagtgcct gtaatgttct gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa
    20641 atgctttttc ctgcactatg acacaatttt gtgtcaggtt gcatataccg aagccgtcgg
    20701 cgggtaacat ttgtatcaat ggttaaacgc gcagcttaaa agatcaggga tttgcgctat
    20761 tgcatttcgc ttagcgactt cgcgccctgg ttatgttgaa tggcgtggcg ttattgcctc
    20821 tgttcgcacc gtggagcggc atacccagac cgttgtatgc tttatttgcg gggcgaaagg
    20881 ggatgcccgc cattgcgcga tattttatgg atgagttaac cacccggctt gccaacgggg
    20941 tctgaatcgc tttttttgta tataatgcgt gtgaaatttc ataccacagg cgaaacgatc
    21001 atgacgcagg atgaattgaa aaaagcagta ggatgggcgg cacttcagta tgttcagccc
    21061 ggtaccattg ttggtgtagg tacaggttcc accgccgcac actttattga cgcgctcggc
    21121 acgatgaaag gccagattga aggggccgtt tccagttcag atgcttccac tgaaaaactg
    21181 aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg acagccttgg catctacgtt
    21241 gatggcgcag atgaaatcaa cggccacatg caaatgatca aaggcggcgg cgcggctctg
    21301 acccgtgaaa aaatcattgc ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc
    21361 aagcaggttg atattctggg taaattcccg ctgccagtag aagttatccc gatggcacgt
    21421 agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg
    21481 gtgaccgata atggcaacgt gatcctcgac gtccacggca tggaaatcct tgacccgata
    21541 gcgatggaaa acgccataaa tgcgattcct ggcgtggtga ctgttggctt gtttgctaac
    21601 cgtggcgcgg acgttgcgct gattggcaca cctgacggtg tcaaaaccat tgtgaaatga
    21661 tctgacgggg gaacctcccc cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt
    21721 cacgctttta ccaggcaatt gtcgattgct ctaaataaat cctctaaacc agcatattca
    21781 tccaagaatt acctttgcgt gatatttcct caacatcgcg acgcaaacgt tcatattgcc
    21841 gcaatattat tttttgatat gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa
    21901 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
    21961 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
    22021 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
    22081 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaaa
    22141 ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg ttgatctgga tgcggcggca
    22201 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
    22261 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
    22321 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
    22381 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
    22441 tatgtttact tttatgatat tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag
    22501 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
    22561 tccaccaaaa atatgatggg cgcgaaagag atttcgctaa tgaagcccgg ctcgctgctg
    22621 attaatgcct cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
    22681 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
    22741 ccatttacct ctccactgtg tgaattcgac aacgtccttc tgacgccaca cattggcggt
    22801 tcgactcagg aagcgcagga gaatatcggc ttggaagttg cgggtaaatt gatcaagtat
    22861 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
    22921 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
    22981 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
    23041 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
    23101 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
    23161 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    23221 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    23281 cgttgtgttt atttaatggg gatttacgat ggcgggacag catggggctg taacggtaaa
    23341 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    23401 attgtactgt tactgataag tttcggagct tattaacagt cagtctcaag ggaggagtca
    23461 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    23521 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    23581 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgagcac
    23641 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    23701 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    23761 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    23821 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    23881 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    23941 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    24001 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    24061 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    24121 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    24181 cagttttggc tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    24241 caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    24301 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    24361 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    24421 ttgacccatt tccagctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    24481 gcggatttct tgtcgggata atgtcagtgg gagctctggt agttgcgtca tggctggtgc
    24541 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    24601 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg aaccgagcat gctcaccaac
    24661 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    24721 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    24781 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    24841 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtctttgct
    24901 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    24961 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    25021 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    25081 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    25141 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    25201 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    25261 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    25321 tctgaaccaa caagggaagg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    25381 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    25441 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    25501 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    25561 cagcgttttc gatcgggctg atgcactggc aggttgggtc aatcacttcc tgcttggtct
    25621 tggcgttacg caaccgaagc tggacaaagt gaccggcgaa accggtgaag ccatcgacga
    25681 tctgcgtaac atcgcgcagc tgggctacga cgaagacgaa gatcaggaag agcttgaaat
    25741 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    25801 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    25861 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    25921 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    25981 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    26041 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    26101 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    26161 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    26221 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    26281 atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    26341 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    26401 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    26461 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    26521 aaattcacca cgaatttaac cgccacggtg cgcgctatcc ttcctataac accattgtag
    26581 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    26641 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    26701 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    26761 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    26821 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggta
    26881 atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc
    26941 actggttagg actggatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    27001 tggaaccggg gatggtactg accgtagagc ctggtctgta tatcgcgccg gatgcggatg
    27061 tgccagaaca atatcgcggt atcggcattc gcattgaaga cgacattgtg attaccgaaa
    27121 ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    27181 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    27241 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    27301 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    27361 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggctg attgcgcaac
    27421 cgccatcact accgtgcatg tcagcgatcg tggtcatgct ggattcgtca ccctcgccgc
    27481 agaagattac caactggcgg cgctggggca ggttgtcgaa ttgcataatg tcgggcaacg
    27541 gctgtttgca ttgctgcgaa aagcacctgg cgtaatgctg cattgccccg atcgcgtagc
    27601 taacgttgcc cgtactcaga gtcatgttga agtgacgctg gagagcggcg agatgctgac
    27661 gggccgcgtg ctgatagccg ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    27721 tgattggcag caggagcctt acgaacaact ggcagtgatt gccaacgttg ctacttctgt
    27781 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    27841 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    27901 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tctgcctttg gctggcgact
    27961 ggggcaaatt acccacgccg gtaaacgcag tgcttatccg ctggcattaa cccgcgccgc
    28021 cagaccgatt acccatcgca ctgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    28081 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    28141 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    28201 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    28261 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    28321 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    28381 gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg
    28441 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    28501 agcgcgtacc ggaacctctg gcggtagatg cactaccaca actgcgcgtt tcggctatca
    28561 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctcgctcgta
    28621 gggccagttg ttatcacggc atggaagtgt gggacaaaga cagttttggt cacatctcgt
    28681 ttgacgatca aagcatgggc tatagccatc ttgggcacat cgttgaaaac tcagtgattc
    28741 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat tactctgttg gcccccacag
    28801 aattacagca ggtcgcctgg ggagaaaatg aaaccttcct gacgctgaaa gatggcagca
    28861 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    28921 cagatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    28981 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    29041 ttttaccgct tagcgatccg catctttgct cgattgtctg gtcactgtcg ccagaggaag
    29101 cgcagcggat gcagcaggca agtgaagacg aatttaatcg cgcgttaaat atcgcttttg
    29161 ataatcgcct tggcttatgc aaggttgaga gcgcgcgtca ggtgttcccg ctgacggggc
    29221 gttatgcgcg ccagtttgcc gcacaccgtc tggcgctggt gggcgacgcc gcgcatacca
    29281 ttcacccact ggcggggcag ggcgttaatc tcggatttat ggacgccgca gagctgattg
    29341 ctgaactgaa acggttgcat cgtcagggta aagacatcgg gcagtacatt tacctgcgcc
    29401 gctatgaacg tagccgcaag cacagcgcgg cgctgatgct ggctggtatg cagggttttc
    29461 gcgatctctt ttccggcgct aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    29521 tggccgacac gcttcctggc gttaagccgc aacttatccg ccaggcaatg ggattaaacg
    29581 atttgcctga atggctgcgt taaaaatttc tcctctgttg tttatttgat acccatcaca
    29641 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    29701 ctccgttttc gcattatatt ttctaatacc atgatttttt gatttagtgt tttttgacat
    29761 ttttttagct cttaatattg tcttattcaa attgactttc tcatcacatc atctttgtat
    29821 agaaattggt gtattttttg gttttttatt ctgtcgcgat ttttgcattt tttaaccata
    29881 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    29941 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt tttgtggtga
    30001 taatttcacc atgaaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac
    30061 agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct
    30121 ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg
    30181 ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct ccgcggcagc cgcacctggg
    30241 agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc
    30301 tttactcggg gatgttgaat gcctctggcg gtgtgataga tgatctcatc gtctactact
    30361 ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct
    30421 ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca
    30481 tgattgccgt gcaagggccg aatgctcagg caaaagctgc cacactgttt aatgatgctc
    30541 agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggtgggc gatctgttta
    30601 ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag
    30661 cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa gccctgcggt ctgggggcgc
    30721 gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta
    30781 tttctccttt agccgccaac atgggctgga ctatcgcctg ggaaccgaca gatcgtgact
    30841 ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc
    30901 tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc
    30961 agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca
    31021 gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca
    31081 accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg
    31141 cgtgatttac ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag
    31201 caaagaacac gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga
    31261 acatgctcag gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac
    31321 ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt cagacattta
    31381 tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact
    31441 ggtgaacagc gaaccgtatg caggcggctg gatctttaaa atcaaagcca gcgatgaaag
    31501 cgaactggaa tcactgctgg atgcgaccgc atacgaagca ttgttagaag atgagtaacg
    31561 gctttattcc tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac
    31621 tctcttcgta aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga
    31681 cacagacgtt aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg
    31741 acgccgcgca acagcaagaa atgctgaatg ccgttggtgc acaatcgtta aacgcgctga
    31801 ccggccagat tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt ggcgcaccgg
    31861 cgaccgaata cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca
    31921 cgtcttacat cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca
    31981 tgctggaaaa tccgggctgg tataccgcgt acactccgta tcagcctgaa gtctcccagg
    32041 gccgccttga agcactgctc aacttccagc aggtaacgct ggatttgact ggactggata
    32101 tggcctctgc ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga
    32161 aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt ggcttccaat gtgcatccgc
    32221 aaacgctgga tgtggtccgt actcgtgctg aaacctttgg ttttgaagtg attgtcgatg
    32281 acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca
    32341 ctaccggtga aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg
    32401 tggtcagcgt tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg
    32461 gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc
    32521 cacacgcggc attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta
    32581 tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg
    32641 agcaacatat tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg
    32701 caaacatcgc cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta
    32761 accgcattca ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa ggtctgaaac
    32821 tgcgccatgc gcactatttc gacaccttgt gtgtggaagt ggccgacaaa gcgggcgtac
    32881 tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gccgtgggga
    32941 tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagt gtgctgctgg
    33001 gcgataacca cgggctggag atcgacacgc tggacaaaga cgtggttcac gacagccgct
    33061 ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct
    33121 atcacagcga aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc
    33181 tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga
    33241 tgatcccaat cacttggacg gaatttgccg aactgcaccc gttttgcccg ccggaacagg
    33301 ctgaaggtta tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt
    33361 acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg ggcctgctgg
    33421 cgattcgtca ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg
    33481 cttctgcgca cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg
    33541 tggcgtgtga taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg
    33601 cgggcgataa cctctcctgt atcatggtga cttatccttc tacccacggc gtgtatgaag
    33661 aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg
    33721 gcgcgaacat gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt
    33781 cgcacctcaa cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg
    33841 gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa
    33901 tcgaaggcat gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta
    33961 tcctgccaat cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa
    34021 gccaggtggc aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg
    34081 tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc
    34141 tgaaagaaga aaccggcatc agcgagctgg atattgccaa gcgcctgatc gactacggtt
    34201 tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat
    34261 ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa
    34321 ttgaccaggt gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc
    34381 acattcagag cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat
    34441 tcccggcagg cgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg
    34501 gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt
    34561 cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagtgc
    34621 tcagacagtc ccctcgcccc tttggggaga gggttagggt gaggggaaca ggccggcact
    34681 ggcgcgaata tttaccctca ccccgaccct ctccctgaaa gggcgagggg gaaaagcgtg
    34741 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt
    34801 tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg
    34861 ggcaactgca ttactgttgg cgcaagaagg gtatacggtg gcggttaatt atcagcaaaa
    34921 cctccacgcg gcgcaggaag tgatgaactt aataatgcaa gccggtggca aagcattcgt
    34981 gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg atgtttacag caatcgatca
    35041 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
    35101 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata
    35161 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
    35221 cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga
    35281 ttacgcggca tcgaaagggg cgattgattc gttaaccacc ggactatcgc tggaagtcgc
    35341 cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc
    35401 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
    35461 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
    35521 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
    35581 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc
    35641 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
    35701 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa
    35761 tgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt
    35821 cactgctttt ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
    35881 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
    35941 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
    36001 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga
    36061 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
    36121 aagataatcg caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt
    36181 aaatccgcga cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac
    36241 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
    36301 acatgagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
    36361 aatgcgacgc gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg
    36421 gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc
    36481 aaacagcggc gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt
    36541 gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa
    36601 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
    36661 gagggttatc accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa
    36721 cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc
    36781 gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
    36841 gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt
    36901 ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc
    36961 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa
    37021 gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta
    37081 caggaaatag taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat
    37141 tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg
    37201 tgatgtgctt cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac
    37261 cgcaacctca accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat
    37321 gaccctgcct gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta
    37381 tatgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt
    37441 taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt
    37501 tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg
    37561 agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg
    37621 ccaacagcgt cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac
    37681 tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg
    37741 gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac
    37801 gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa
    37861 cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg
    37921 ttatctggag cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca
    37981 aaattttatc tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg
    38041 aaatggcagt taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt
    38101 attcgctcgg ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc
    38161 acggcttcgt gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc
    38221 aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc
    38281 ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt
    38341 tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata
    38401 aatgcctgat gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc
    38461 agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg
    38521 acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta
    38581 cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg
    38641 gcactacctg ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc
    38701 accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga
    38761 ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct
    38821 tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta
    38881 tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt
    38941 tcaaaccata gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt
    39001 tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca
    39061 cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc
    39121 agctgcggtt cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac
    39181 agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga
    39241 tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc
    39301 gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg
    39361 gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt
    39421 gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca
    39481 ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg
    39541 tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa
    39601 cacgttacaa tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa
    39661 caaagcccga attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat
    39721 gccgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg
    39781 tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc
    39841 cgatgcagaa ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc
    39901 tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg
    39961 ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga
    40021 tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc
    40081 gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga
    40141 gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg
    40201 gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg
    40261 atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat
    40321 gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg
    40381 caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga
    40441 cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc
    40501 gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg
    40561 agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata
    40621 attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg
    40681 atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat
    40741 ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg
    40801 attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc
    40861 ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta
    40921 gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac
    40981 aggagctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg
    41041 aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc
    41101 gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc
    41161 tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt
    41221 tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac
    41281 cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac
    41341 aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt
    41401 atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc
    41461 gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg
    41521 aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg
    41581 gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct
    41641 tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac
    41701 cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg
    41761 tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg
    41821 ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa
    41881 gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag
    41941 gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa
    42001 gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg
    42061 tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg
    42121 gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa
    42181 aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata
    42241 ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga
    42301 tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa
    42361 tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta
    42421 aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc
    42481 agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt
    42541 accagccgcc gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta
    42601 aataattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga
    42661 cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag
    42721 tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt
    42781 cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt
    42841 cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc
    42901 gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt
    42961 aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga
    43021 taacggtatt tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat
    43081 tgttaccgat caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc
    43141 taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta
    43201 tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat
    43261 cgcaattcct aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt
    43321 cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc
    43381 cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa
    43441 actagccgcc agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact
    43501 ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt
    43561 gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca
    43621 ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg
    43681 gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat
    43741 caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa
    43801 aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac
    43861 actctacccc ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct
    43921 ggaagaggat aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct
    43981 ggccccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat
    44041 gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga
    44101 gccgctgttt gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt
    44161 gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt
    44221 cgataccgct ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga
    44281 tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat
    44341 ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc
    44401 gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa
    44461 tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg
    44521 ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg
    44581 aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca
    44641 ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag
    44701 aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa
    44761 aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt
    44821 acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg
    44881 agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt
    44941 cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt
    45001 acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact
    45061 ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg
    45121 atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg
    45181 cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg
    45241 ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga
    45301 agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg
    45361 cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg
    45421 atgactcccg cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc
    45481 tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca
    45541 acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga
    45601 aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt
    45661 tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag
    45721 aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta
    45781 tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg
    45841 cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg
    45901 acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact
    45961 gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct
    46021 tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat
    46081 cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga
    46141 accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg
    46201 cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc
    46261 cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc
    46321 gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac
    46381 tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta
    46441 ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact
    46501 tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa
    46561 ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta
    46621 tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg
    46681 cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc
    46741 tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg
    46801 gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct
    46861 tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag
    46921 attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg
    46981 accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg
    47041 cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt
    47101 ttttatttaa gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc
    47161 atcaccatcc acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct
    47221 aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg
    47281 cgtgcggcaa ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg
    47341 tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg
    47401 caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag
    47461 ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc
    47521 cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt
    47581 gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt
    47641 tccgtttgca taatttctca catgtaattc tgatcactca tcattataga taattgaaga
    47701 gtgaaatgat taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac
    47761 aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg
    47821 caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag
    47881 cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga
    47941 agccacatac gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg
    48001 tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg
    48061 agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg
    48121 tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa
    48181 cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg
    48241 aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc
    48301 atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg
    48361 ctatgaacgc gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag
    48421 ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata
    48481 tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac
    48541 tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg atcgaaaatc
    48601 ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg tagcccggat
    48661 aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc cagtaatacg
    48721 gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagacg
    48781 gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt acctccagcg
    48841 gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta
    48901 accagtggcg ggaataaagg catcaagcga ccaataagtg gcgctaataa tgtggtgata
    48961 aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca
    49021 atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc
    49081 cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga
    49141 tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata
    49201 agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc
    49261 agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat
    49321 gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagaataaa
    49381 aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg
    49441 cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca
    49501 cgaaaatgac aagagaacct gacaatggaa ttatctgtaa ttttctcata ttgatatttc
    49561 attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg atagcgctct
    49621 tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact
    49681 cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc
    49741 gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat aagtttatcg
    49801 ctgctgaagc tgcggaatgt ataggctgtc a
//
LOCUS       DAECOD010000019        48071 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.019, whole genome shotgun sequence.
ACCESSION   DAECOD010000019 DAECOD010000000
VERSION     DAECOD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.019"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..497
                     /gene="ftnA"
                     /locus_tag="KKO15_001098"
     CDS             <1..497
                     /gene="ftnA"
                     /locus_tag="KKO15_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBD6359975.1"
                     /translation="LKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLRR
                     HAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKINE
                     LAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELSTL
                     DTQN"
     gene            complement(534..773)
                     /locus_tag="KKO15_001099"
     CDS             complement(534..773)
                     /locus_tag="KKO15_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBD6359976.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            963..2174
                     /gene="tyrP"
                     /locus_tag="KKO15_001100"
     CDS             963..2174
                     /gene="tyrP"
                     /locus_tag="KKO15_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBD6359977.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2236..2901)
                     /gene="yecA"
                     /locus_tag="KKO15_001101"
     CDS             complement(2236..2901)
                     /gene="yecA"
                     /locus_tag="KKO15_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBD6359978.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3097..3183)
                     /locus_tag="KKO15_001102"
     tRNA            complement(3097..3183)
                     /locus_tag="KKO15_001102"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3147..3149),aa:Leu,seq:taa)
     gene            complement(3196..3269)
                     /locus_tag="KKO15_001103"
     tRNA            complement(3196..3269)
                     /locus_tag="KKO15_001103"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3235..3237),aa:Cys,seq:gca)
     gene            complement(3324..3399)
                     /locus_tag="KKO15_001104"
     tRNA            complement(3324..3399)
                     /locus_tag="KKO15_001104"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3364..3366),aa:Gly,seq:gcc)
     gene            complement(3551..4099)
                     /gene="pgsA"
                     /locus_tag="KKO15_001105"
     CDS             complement(3551..4099)
                     /gene="pgsA"
                     /locus_tag="KKO15_001105"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBD6359979.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4156..5988)
                     /gene="uvrC"
                     /locus_tag="KKO15_001106"
     CDS             complement(4156..5988)
                     /gene="uvrC"
                     /locus_tag="KKO15_001106"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBD6359980.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5985..6641)
                     /gene="uvrY"
                     /locus_tag="KKO15_001107"
     CDS             complement(5985..6641)
                     /gene="uvrY"
                     /locus_tag="KKO15_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBD6359981.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6937..7113
                     /locus_tag="KKO15_001108"
     CDS             6937..7113
                     /locus_tag="KKO15_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6359982.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            7100..7324
                     /locus_tag="KKO15_001109"
     CDS             7100..7324
                     /locus_tag="KKO15_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBD6359983.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7357..7977)
                     /gene="sdiA"
                     /locus_tag="KKO15_001110"
                     /pseudo
     CDS             complement(7357..7977)
                     /gene="sdiA"
                     /locus_tag="KKO15_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(8207..8959)
                     /gene="yecC"
                     /locus_tag="KKO15_001111"
     CDS             complement(8207..8959)
                     /gene="yecC"
                     /locus_tag="KKO15_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBD6359984.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(8956..9624)
                     /gene="tcyL"
                     /locus_tag="KKO15_001112"
     CDS             complement(8956..9624)
                     /gene="tcyL"
                     /locus_tag="KKO15_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBD6359985.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9639..10625)
                     /gene="dcyD"
                     /locus_tag="KKO15_001113"
     CDS             complement(9639..10625)
                     /gene="dcyD"
                     /locus_tag="KKO15_001113"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBD6359986.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10730..11530)
                     /gene="tcyJ"
                     /locus_tag="KKO15_001114"
     CDS             complement(10730..11530)
                     /gene="tcyJ"
                     /locus_tag="KKO15_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6359987.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11618..12169)
                     /gene="fliZ"
                     /locus_tag="KKO15_001115"
     CDS             complement(11618..12169)
                     /gene="fliZ"
                     /locus_tag="KKO15_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBD6359988.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12215..12934)
                     /gene="fliA"
                     /locus_tag="KKO15_001116"
     CDS             complement(12215..12934)
                     /gene="fliA"
                     /locus_tag="KKO15_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBD6359989.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13099..14751)
                     /gene="fliC"
                     /locus_tag="KKO15_001117"
     CDS             complement(13099..14751)
                     /gene="fliC"
                     /locus_tag="KKO15_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBD6359990.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            15017..16423
                     /gene="fliD"
                     /locus_tag="KKO15_001118"
     CDS             15017..16423
                     /gene="fliD"
                     /locus_tag="KKO15_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD6359991.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16448..16858
                     /gene="fliS"
                     /locus_tag="KKO15_001119"
     CDS             16448..16858
                     /gene="fliS"
                     /locus_tag="KKO15_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBD6359992.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            16858..17223
                     /gene="fliT"
                     /locus_tag="KKO15_001120"
     CDS             16858..17223
                     /gene="fliT"
                     /locus_tag="KKO15_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBD6359993.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17301..18788
                     /gene="amyA"
                     /locus_tag="KKO15_001121"
     CDS             17301..18788
                     /gene="amyA"
                     /locus_tag="KKO15_001121"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD6359994.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18822..19235)
                     /locus_tag="KKO15_001122"
     CDS             complement(18822..19235)
                     /locus_tag="KKO15_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6359995.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19422..20627
                     /gene="yedE"
                     /locus_tag="KKO15_001123"
     CDS             19422..20627
                     /gene="yedE"
                     /locus_tag="KKO15_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBD6359996.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            20624..20857
                     /gene="yedF"
                     /locus_tag="KKO15_001124"
     CDS             20624..20857
                     /gene="yedF"
                     /locus_tag="KKO15_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBD6359997.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            20964..21634
                     /locus_tag="KKO15_001125"
                     /pseudo
     CDS             20964..21634
                     /locus_tag="KKO15_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(21674..22882)
                     /locus_tag="KKO15_001126"
                     /pseudo
     CDS             complement(21674..22882)
                     /locus_tag="KKO15_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22950..23565
                     /gene="tnpA"
                     /locus_tag="KKO15_001127"
                     /pseudo
     CDS             22950..23565
                     /gene="tnpA"
                     /locus_tag="KKO15_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            23893..24975
                     /locus_tag="KKO15_001128"
     CDS             23893..24975
                     /locus_tag="KKO15_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBD6359998.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(25464..26262)
                     /locus_tag="KKO15_001129"
                     /pseudo
     CDS             complement(25464..26262)
                     /locus_tag="KKO15_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(26272..26823)
                     /gene="ybcL"
                     /locus_tag="KKO15_001130"
     CDS             complement(26272..26823)
                     /gene="ybcL"
                     /locus_tag="KKO15_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBD6359999.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(26992..27324)
                     /gene="emrE"
                     /locus_tag="KKO15_001131"
     CDS             complement(26992..27324)
                     /gene="emrE"
                     /locus_tag="KKO15_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBD6360000.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(27669..27983)
                     /gene="fliE"
                     /locus_tag="KKO15_001132"
     CDS             complement(27669..27983)
                     /gene="fliE"
                     /locus_tag="KKO15_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBD6360001.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            28197..29862
                     /gene="fliF"
                     /locus_tag="KKO15_001133"
                     /pseudo
     CDS             28197..29862
                     /gene="fliF"
                     /locus_tag="KKO15_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            29855..30850
                     /gene="fliG"
                     /locus_tag="KKO15_001134"
                     /pseudo
     CDS             29855..30850
                     /gene="fliG"
                     /locus_tag="KKO15_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            30843..31529
                     /gene="fliH"
                     /locus_tag="KKO15_001135"
     CDS             30843..31529
                     /gene="fliH"
                     /locus_tag="KKO15_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBD6360002.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            31529..32902
                     /gene="fliI"
                     /locus_tag="KKO15_001136"
     CDS             31529..32902
                     /gene="fliI"
                     /locus_tag="KKO15_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBD6360003.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            32924..33265
                     /gene="fliJ"
                     /locus_tag="KKO15_001137"
                     /pseudo
     CDS             32924..33265
                     /gene="fliJ"
                     /locus_tag="KKO15_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            33262..34389
                     /gene="fliK"
                     /locus_tag="KKO15_001138"
     CDS             33262..34389
                     /gene="fliK"
                     /locus_tag="KKO15_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBD6360004.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSVPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            34494..34958
                     /gene="fliL"
                     /locus_tag="KKO15_001139"
     CDS             34494..34958
                     /gene="fliL"
                     /locus_tag="KKO15_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBD6360005.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            34963..35967
                     /gene="fliM"
                     /locus_tag="KKO15_001140"
     CDS             34963..35967
                     /gene="fliM"
                     /locus_tag="KKO15_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBD6360006.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            35964..36377
                     /gene="fliN"
                     /locus_tag="KKO15_001141"
     CDS             35964..36377
                     /gene="fliN"
                     /locus_tag="KKO15_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBD6360007.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            36380..36745
                     /gene="fliO"
                     /locus_tag="KKO15_001142"
     CDS             36380..36745
                     /gene="fliO"
                     /locus_tag="KKO15_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBD6360008.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            36745..37482
                     /gene="fliP"
                     /locus_tag="KKO15_001143"
     CDS             36745..37482
                     /gene="fliP"
                     /locus_tag="KKO15_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBD6360009.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            37492..37761
                     /gene="fliQ"
                     /locus_tag="KKO15_001144"
     CDS             37492..37761
                     /gene="fliQ"
                     /locus_tag="KKO15_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBD6360010.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            37770..38555
                     /gene="fliR"
                     /locus_tag="KKO15_001145"
     CDS             37770..38555
                     /gene="fliR"
                     /locus_tag="KKO15_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBD6360011.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            38840..39463
                     /gene="rcsA"
                     /locus_tag="KKO15_001146"
     CDS             38840..39463
                     /gene="rcsA"
                     /locus_tag="KKO15_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBD6360012.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(39507..39695)
                     /gene="dsrB"
                     /locus_tag="KKO15_001147"
     CDS             complement(39507..39695)
                     /gene="dsrB"
                     /locus_tag="KKO15_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBD6360013.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            39858..40085
                     /gene="yodD"
                     /locus_tag="KKO15_001148"
     CDS             39858..40085
                     /gene="yodD"
                     /locus_tag="KKO15_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBD6360014.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            40383..41198
                     /locus_tag="KKO15_001149"
                     /pseudo
     CDS             40383..41198
                     /locus_tag="KKO15_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(41195..42889)
                     /gene="yedQ"
                     /locus_tag="KKO15_001150"
                     /pseudo
     CDS             complement(41195..42889)
                     /gene="yedQ"
                     /locus_tag="KKO15_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(43060..43242)
                     /locus_tag="KKO15_001151"
     CDS             complement(43060..43242)
                     /locus_tag="KKO15_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBD6360015.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(43321..44238)
                     /locus_tag="KKO15_001152"
     CDS             complement(43321..44238)
                     /locus_tag="KKO15_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBD6360016.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            44411..45331
                     /gene="yedA"
                     /locus_tag="KKO15_001153"
     CDS             44411..45331
                     /gene="yedA"
                     /locus_tag="KKO15_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBD6360017.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(45320..45790)
                     /gene="vsr"
                     /locus_tag="KKO15_001154"
     CDS             complement(45320..45790)
                     /gene="vsr"
                     /locus_tag="KKO15_001154"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBD6360018.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(45771..47189)
                     /gene="dcm"
                     /locus_tag="KKO15_001155"
     CDS             complement(45771..47189)
                     /gene="dcm"
                     /locus_tag="KKO15_001155"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBD6360019.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(47256..47796)
                     /locus_tag="KKO15_001156"
                     /pseudo
     CDS             complement(47256..47796)
                     /locus_tag="KKO15_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(47836..>48071)
                     /locus_tag="KKO15_001157"
     CDS             complement(47836..>48071)
                     /locus_tag="KKO15_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360020.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        11944 a        12381 c        11757 g        11989 t
ORIGIN      
        1 tgctgaaacc agaaatgatt gaaaaactta atgagcagat gaacctggaa ctgtactctt
       61 cactgcttta tcagcaaatg agcgcctggt gcagctatca taccttcgaa ggtgctgccg
      121 cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg tttgattacc
      181 tgactgatac cggcaattta ccgcgtatta ataccgttga atctccgttt gctgaatatt
      241 cctcacttga tgaattattc caggaaacct ataaacacga acaattaatc acccagaaaa
      301 ttaacgaact ggctcatgct gcaatgacca atcaggacta cccaacattt aatttcctgc
      361 agtggtatgt ttctgagcag catgaagaag agaaactgtt caaatcgatt attgataaat
      421 taagcctggc aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc
      481 tcgacacaca aaactaatgc tctccggcgg caggtttctg ccgccgtttc aaatcagtga
      541 cggcaaatct tactctgatc ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt
      601 gctgccagaa acgctaccag ctctcccgct gtcacccctt ctgccgagca cgtgtgaaaa
      661 cgtgcctgtt caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc
      721 tcgcctgatt caatcatcat atttaacact tcatgaccgt gaatagagtc catcgttcct
      781 cctcaaaaaa agcctagcgt agcgattacc gcttatgaag actttgcgcc agcgcaggac
      841 tgaatgcttt tattgtacat ttatatttac acaatatgta acgtcggttt gacgaagcag
      901 ccgttatgcc ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa
      961 gcgtgaaaaa cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag
     1021 gcatgctggc aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt
     1081 tgattgggct ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc
     1141 atgttccggc agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc
     1201 aatggctgac gggcttcagt atgatgttct taatgtatgc tctgactgca gcatacatca
     1261 gcggtgccgg tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg
     1321 caaccgctgg cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat
     1381 cgctggtcga tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga
     1441 tgctggtatt gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac
     1501 aggggctggc actgtctgca atcccggtga tttttacgtc gtttggtttt cacggtagcg
     1561 tgccgagtat tgtcagctat atggatggca acattcgtaa gctacgctgg gtgtttataa
     1621 tcggtagtgc catccccctg gtggcatata ttttctggca ggtggcgacg cttggcagca
     1681 ttgattcaac aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac
     1741 aggcgttacg cgaaatggtg gcctctccgc atgttgagct ggcggtgcat ttatttgctg
     1801 atttagccct cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg
     1861 atttgtttca gcgttcaaat accgttggtg gacgattgca aactggtgca attacctttc
     1921 tgccgccgtt ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg
     1981 ccggtgtggc gctggcggta ctggcattga ttatcccttc actattgacc tggcaaagca
     2041 gaaagcacaa tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg
     2101 tgtttctctg tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac
     2161 cagaagtggg gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac
     2221 acccaatgtg acctcttaat gcaggcagca ctgcttaaat ttcttaccac tgccgcacgg
     2281 acaaggatcg ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc
     2341 ctgcggatgc gccatccagt atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac
     2401 gctctcttca aacgcttctg gcgacatttt ctctacccgc tcgaagtttt cctcagtacc
     2461 gtgcagcgca atcgcctcca gcgctggttt taaagaatcg ggcaacgttg accagtcaga
     2521 aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc
     2581 atcaacttct cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc
     2641 tgcggtatcg gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga
     2701 cgcccaacgc ggcacatagt cagccccacc ccacacggca accagccact gttctggttc
     2761 aatctcttgc ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag
     2821 gatggcgtgg tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc
     2881 gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc
     2941 ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta
     3001 aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca
     3061 ctgcttatta ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac
     3121 ccgcacagcg cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg
     3181 ggctcgggaa gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat
     3241 ccgctacata accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg
     3301 cttgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg agaccccgac
     3361 cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa
     3421 tcacttgatt ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact
     3481 tacctggcgc gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac
     3541 gccgaaacga tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg
     3601 accacagagt cagtaccgca gccacaaaga aaagtgcaat accgacgtac tcaacccaaa
     3661 tgttcggacg ccacagcagc caggccaacg ccaccatctg ggcagtggtt ttcactttcc
     3721 caatccagga gacggccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg
     3781 cagaaataat aatttcacgg gcgatcatcg ttgccgccgg taatgtcacc caccagctgt
     3841 gataatgctc ggttaccagc accatggcga tagccacgag aactttatct gccacagggt
     3901 caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc
     3961 agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag
     4021 gcagataaaa gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg
     4081 tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat
     4141 gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga
     4201 aatacccggc acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc
     4261 catatatttc aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga
     4321 actggtattt ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga
     4381 ttcatcgcga atatgctgga taacatgcag cgcgggtgaa tctggcggca aactaaatcc
     4441 ctcaccttcc ggctcaaaga acagcgtttc cagtccagcc ttacgatctg ctcctttggc
     4501 aacgccaagt agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt
     4561 tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact
     4621 gtcgtcaatg gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc
     4681 gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc
     4741 atcaaacacc acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca
     4801 ctccatccgc ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg
     4861 aacggtagat tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag
     4921 tttcagataa cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc
     4981 cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat
     5041 ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactgaccta cgaaggtttc
     5101 taccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag
     5161 cactttgccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac
     5221 accaataacg tcaaggtcgt cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg
     5281 caccgcctga atttggtcgc gaatacatgc cgcttcttca aactccagat tctggctggc
     5341 agtttccata cggctaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag
     5401 gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg
     5461 tcccagacag cgccctatct ggtattgcag acacggacgc gagcgattgc gataaacact
     5521 attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc
     5581 atagccattc gggaacgggc cgaaatattc acctttggca tgctttgcac cacgatgcat
     5641 cgccagacgc gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg
     5701 tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc
     5761 ttcggtttct gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc
     5821 ggttttgcgc gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt
     5881 cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg
     5941 gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat
     6001 gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga
     6061 atgtttagtt tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg
     6121 agctgttctg agatctcatt gaccttctgg cccttagtga tcatcagcat aatctgcaat
     6181 tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac
     6241 gccatttgtt gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc
     6301 gcactcacga cttcctgcgg agccgcgcct ttgctgaggt agccagcagc acctgcctgc
     6361 atgactttcg ctggtaaagg gttttctgta tggaccgtaa gcatgatgat tttaacatca
     6421 gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg
     6481 tccattagca ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac
     6541 gatgcctctc cgacgacttt tataccctta atatcttcca gaatgcgtcg tatccctgcg
     6601 cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata
     6661 gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa
     6721 acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag
     6781 ttatggtcac gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctacgatga
     6841 aatgtgacaa aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac
     6901 attaattaac ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat
     6961 tatattaacg tattttatag cactgccgat atcacgctca aaaaacaacc actgctattt
     7021 ttagcaaagc ttatggtata ctccgccgcc ttaacatttt tcaccacaaa ttttcattgc
     7081 aacatgacga ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga
     7141 actggcaaat gaagtctcct gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg
     7201 acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga
     7261 cgaaacccag gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca
     7321 gtaatataaa accggctgag agcgtgcctt tcagccggtt attgacaaaa tcatcgctat
     7381 ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa
     7441 attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc
     7501 gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt
     7561 gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt
     7621 gacaccgcgg cgtaaaccat gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa
     7681 taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca gcacaggatc
     7741 aattgcgaga aaattttttg cctgataata actaacccac gactcagggt aattggtgta
     7801 gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc
     7861 gtactccagc tgctgagcct gaagctctat ttcatggtag gcctcttctg cggcctccat
     7921 cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt
     7981 aaaccgcaac gcccctgaga gtgagtatca tttataatga taattcaaac tatcacataa
     8041 ataagattta tatataattt atattattca ggcaatgaat tacttttgca agccatcgca
     8101 ttctcttatg ttattaatga gttatgctga tttgttaagc agttttatca cgcttgaaat
     8161 ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa
     8221 gcttctcgag gaactggcgg gtgcgaggct gctggggatc ggcaaataac gcttttgctg
     8281 ccccctgctc gactatccgc ccctggtcca taaagatcgc ccggtccgca acatcccggg
     8341 caaagctcat ttcgtgtgtc acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga
     8401 tggtgttcag gacttcgccc accagctctg gatccagcgc cgacgttggc tcgtcaaaca
     8461 aaatcacctc aggacgcatt gccagtgcac gcgcaatcgc aacacgctgc tgttgaccgc
     8521 cagacaaacg acgtggatag ctggtttctt taccggccag cccaactttc gccagcagct
     8581 cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa
     8641 taatgttttc cagcaccgta cgatgcggaa acaaattaaa gttctggaag acaaacccga
     8701 cgtgctgacg caactggcga atcagcgatt tttgctgact taatgaacgt gcagtatcaa
     8761 tagtgatatc gccaacggtg atcgttcccg cttccggttg ttccagcaga tttatgctac
     8821 gtagcaatgt ggttttgccg gaaccactcg gaccgataat tgccaccact tcgccaggct
     8881 ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg gaattttttc accaggttct
     8941 taacttcaat ggcactcatt ttggttctct ctcctggcga ttaagttgat tctcaaaatg
     9001 gttctgcaac gtcgataaca cagtcgccat gatccagtag atgagcgaag ccgccaggta
     9061 catggtgaac acctccagcg tgcgcgaggt aatcaactgc gcctgacgga acagctccgg
     9121 cacctggatt gtcgcggcca gcgacgtatc ttttaccaga ctgataaagc tgttcgacag
     9181 cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg
     9241 cgtcatacca atactggcag cggcttccca ctgaccttta tcaatagaag aaatggcggc
     9301 acgcagcgtt tcggcggcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg
     9361 aatgggatct aattcgatac caaactgcgg caggccgtaa tagatcataa acagttgtgc
     9421 gatgagcggc gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca
     9481 gattggcgac aggcgcatca gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc
     9541 gccgatactg agttgcaggg tatacccagc ccctttcaac aggaacggca aggaatcaat
     9601 aaccagttgt atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg
     9661 aacagcgcag gcgcgccgcc ggtatgaata aacagaatcg gcccttcatc tttgaagcgt
     9721 ttctgactga taccgtcaat cagccccgcc atcgcttttc cggtatacac aggatcaagc
     9781 agaatgcctt ccagacgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg
     9841 ccgtagccag gtgcaaaata gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc
     9901 agttctttcg caatcgcctg ttgtaggtta accactttcg gcaattgatc ggcaacggaa
     9961 cgcgacacgg tcacgccaat cagttcgctt tcaggcatca ggtgttccag cccaacagcc
    10021 agtccggcgt gagttccggc actgcccgat gcgactacca ccgacgaaat attaaccgcc
    10081 ccttcacact gttgcgcgat ttccagcgca ctctccacat aacctagcgc gccaagagca
    10141 ttagaaccgc caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc
    10201 agttcttcca gttgggcatt gggatcggtc agtgcgtcgc acatttcaat ctgggtattg
    10261 aacagatcca gcaacaaacg attgccgttg gttaaatagt tttctgcggt tgtgccaata
    10321 ggattttcca gcagcgccac gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg
    10381 cgcacatggt tagactggat cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg
    10441 gcatctgccg cgagaaattc cagcttacgt aatttattgc cgcccattgc catgggggtg
    10501 acctcatccc gtttgatgaa aatttcccgt ccaagataat cagaaaagcg cggcagatat
    10561 tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa aacgggttaa attatgcagt
    10621 ggcataacag cctccgatgt gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg
    10681 taaataaaaa aggcgctagt gaaagcgccc ttttttgtca ttatgctgat tatttggtca
    10741 catcagcacc aaaccatttt tcggaaagag cttgcagagt gccgtctttt tgcatttccg
    10801 caattgcatc attcaccgct ttcagcaggt cttcatttcc tttacgcagc gccacgccag
    10861 actcctgacg ggagaatgct tcaccggtta ctgccagcgt atcgttggtt ttcttcacca
    10921 gatccagcgc cgccagacga tcaacgagga tcgcatcgat acgccctacg cgcagatcct
    10981 gatatttggt cgggtcatca tcataggtac gcacatcgac accctgaaca ttctgccgca
    11041 gccactcttc atagttggtg cccagaccga cacccacttt tttgcctttc agatcagcag
    11101 ccgttttaat ggtgccttcg ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt
    11161 aaggggtaga gaaatcgtat tttttcttgc gctcgtcaga aatggtgacc tgattaatca
    11221 ccacatcaat gcgtttagag tccagcgacg ccagcatacc gtcccatttg gtcggtttta
    11281 gtgacgcctc aacgccaaga tgttttgcca gctgttgggc aaattccact tcaaaaccgg
    11341 ttaatttgcc gtcatccccc tgaaaactga acggcggata agtgccttcc agccctacca
    11401 gcagcgtgcc gctctctttg actttattaa gcagaccttc atctgcaaaa cttttaacgc
    11461 tcatgcccgc aaccagcgcc acggccatca cacccatcaa tgcctgacgt cccagatgtg
    11521 ctaatttcat attcaccccg aatgttgtta tgtctgtttg cagtgtagag ccattgtttg
    11581 taaacacaaa aacaactccg ctacatctta ttcttattta atatatatca gaagcaggca
    11641 ggctggagga tttctgcaca agtccggtac aggtttggcg ctgatagtgt tgatacttcc
    11701 gtaatgcgat gcggtaattg ttggtgctgg tggtgggcag ccacggggcg aggatttcat
    11761 caaggaaacc gtcctgcagc tgatcgaggg aaatattttg ctcatgtaga tgattaccca
    11821 aacggcgcag acgaacgaca tattcacgca ccgtacctgg gctcatttca gtttgctcaa
    11881 aaagaaattt cttaaagccg ataatatcga aaaaatcact ctgcgttttg caatgtaaat
    11941 caccgcaaaa tcggcacaat gccgcccatg attgttgttc cgtttgccag cctttttcat
    12001 caagcagcgt gtccaggcgg gaaatctcga ttttattcac tattttgccg tcgcgaacta
    12061 aggtaatgcg atcgagtaat ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt
    12121 ctttaagata gcggcttaat ggccgtcttt tcaggtgctg caccatcatt aagaactcct
    12181 ggtagtcaaa gttaaagtgc ggcatttact gacgttataa cttacccagt ttagtgcgta
    12241 accgtttaat ggcctggctg tgtaactgac tgacccgcga ttccccgacc tccagcaccg
    12301 cgccaatctc tttgagattc agctcttcct ggtaatagag ggttaatacc agtttttcgc
    12361 gctccggcaa cgtttcgatg gcttccatca cccgctggcg cagattactg tccagcagtt
    12421 gttgtagcgg gttttctcgc tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct
    12481 cttcacgcca ctcatcgtag gagaagagct ggctgttatt ggtgtcgagc agcatttgac
    12541 gataatcggc aatatcgatc cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc
    12601 caagttcctg ctccagttgc cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc
    12661 tgcgcggcac ccagtcacgg ctgcgaagtt catccagcat agcgccacgg atacgctgca
    12721 ctgcgtaagt tgtaaatgcc gttccttgta gggcgtcata gcgttcgaca gcattaagta
    12781 acccaatgcc gcccgcctgt agcagatcgt caagttccac gctcgcgggc agtcgaacct
    12841 gcaggcgcaa tgcttcgtga cgcaccagcg ggacataacg ctgccacagc gagtgtttat
    12901 ccattacacc ttcagcggta tagagtgaat tcacgataaa cagccctgcg ttatatgagt
    12961 tatcggcatg attatccgtt tctgcagggt ttttaatcgg acgattagtg ggtgaaatga
    13021 ggggttattt gggggttacg caaaaagccg aatgtaaaaa accccgccgg tggcggggtt
    13081 tgagaggcat tcagacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt
    13141 ggcttttgcc agcacggagt taccggcctg ctggataatc tgcgctttcg acatgttgga
    13201 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcagaca ggttggtagt
    13261 ggtgttgttc aggttggtga ctgcggaatc caaacggttt tgcaccgcac ccagggagga
    13321 gcggaatttg tcgatggagc tgatggcttc atccagagct ttcaggggat cggctgtcgt
    13381 agctgctttt gtttcagcat ctgtcgtcaa tttaccatca gcatctttat aaactgcctt
    13441 accgctgccg ttagtcactg aaccatcatc ctgtaggtaa aaatctacag tatcagatgt
    13501 ggtcaacgcc tcgttgttat caacataaac cgaatcaaca ccttttgtta cagcgccagt
    13561 tgtgccgttg acgctataac ctgcagtagt gaaatcaaca ttgtttcccg aggttgatac
    13621 agctttatcg aaagctgcct gatcaacttt tgcacctgtt actgaataag taatagtgtc
    13681 cggcgtagta ctattaccgg taaaggagat agaggtaccg ccaatgtcaa tggttgtcgc
    13741 tttggtatta ttggctgcaa gtgtggacag actagcctca gtcaaagaag caccagagcc
    13801 gtttttcgtt aagcgcccag ttgtatcaat gtagagttta tctccattgc ttgccgtaat
    13861 tttaccatca ctgctgatct gaacatcctg tgcagaacca tcgatagtaa tagtgccctt
    13921 agcggtatca ccaactcccg gggtcaaata tttctgaaca tcggcagctg aagccgttgt
    13981 ggtatcaaaa gagtatgact tacttgcact gtcatacttg taattcgttg cactagccgc
    14041 cgttccgaag ccgttattaa tggttgcctg aacagtatca ccatcactaa ctccagccag
    14101 caaatcagac gctttagcag catcgtaacc cgcactcaca gtatatttgt tgccgaccac
    14161 tgttgcatta gcagctacca agtcagcttt agatgcagca gtattagcca cagccccgcc
    14221 accattcaca ttaaacccat tcagccccag cgtatcagag tcaattttct tcagatcaat
    14281 agtgatagtc tggccgtcat tcgcaccaac ctgaattttc atcgaaccgt cttttgccag
    14341 tacgttcacg ccgttgaact gggtctgacc ggatacgcgg tcaatttcgt cgagacggga
    14401 tttgatttcg tcctgaatgg agtccagatc cgaatcagag ttagtcccgg tagaagcctg
    14461 aaccgtcagc tcacggatac gctgtaagtt gttgttgatt tcggacagcg cgccttcagt
    14521 ggtctgtgca acagaaatac cgtcgttggc gttacgtgca gcctgagtca ggcctttaat
    14581 gttagaagta aaacggttag caatcgcctg acccgcggcg tcatccttcg cgctgttaat
    14641 acgcaagcca gaagacagac gctcgataga actcgacagc gcagactggt tcttgttgat
    14701 attattttga gtgatcagcg agaggctgtt ggtattaatg acttgtgcca tgattcgtta
    14761 tcctatattg caagtcgttg attacgtttt gggtttccac ccgtcggctc aatcgccgtc
    14821 aaccctgtta tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa cagccatttt
    14881 ttgttagtcg acgaaatact cttttctctg ccccttattc ccgctattaa aaaaaacaat
    14941 taaacgtaaa ctttgcgcaa ttcaggccga taaccccggt attcgtttta cgtgtcgaaa
    15001 gataaaagga aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt
    15061 aagttcaatc cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa
    15121 tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac
    15181 gactttccag accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcac
    15241 caccagcagc accaccgcgt tcagtgccac caccgcgggt aacgccatcg ccgggaaata
    15301 caccatcagc gtcacccatc tggcgcaggc gcaaaccctg accacgcgca ccaccagaga
    15361 tgatacgaaa acggcgatcg ccaccagcga cagcaaactc accattcaac aaggcgacga
    15421 caaagatccg attaccattg atatcagcgc ggctaactca tcgttaagcg ggatccgtga
    15481 tgccatcaac aacgcaaaag cgggcgtaag tgcgagcatc attaacgtgg gtaacggtga
    15541 atatcgtctg tcagtcacat caaatgacac cggccttgat aatgcgatga cactctcggt
    15601 cagcggtgat gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg
    15661 tatggaggtc tcggtcgccg cccagaatgc gcagctgacg gtcaacaacg tcgccatcga
    15721 gaacagcagc aacactatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt
    15781 caccacgggc aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat
    15841 taaagactgg gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata
    15901 caccgccgta gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga
    15961 ctccacgctg cggacgattc agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc
    16021 ttccagctat aaaacgctgg cgcagattgg tatcacgacc gatcccagcg atggcaaact
    16081 ggaactggat gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc
    16141 attgattgtt ggcgatggta aaaaaaccgg catcacaacc accatcggca gcaacctgac
    16201 cagttggctt tcgacaacgg gcattattaa agccgctacc gatggcgtta gtaaaaccct
    16261 gaataagtta actaaagact acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg
    16321 ctacaaagaa caatttaccc aactggacgt tttaatgacc tcgttaaaca gcaccagcag
    16381 ctacttaacg cagcagttcg aaaacaacag taattccaag taatcattat tcatcgggag
    16441 acaggtcatg tacgcggcaa aaggcaccca ggcctatgca caaattggcg tcgaaagcac
    16501 cgtaatgagc gccagccaac agcagttggt caccatgcta tttgatggag tgctgagcgc
    16561 actggttaga gcgcgactgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt
    16621 gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg agtcttgatg aagagagcaa
    16681 agacgaatta acccaaaact taattgctct ttatagctat atggtcaggc gtttgctgca
    16741 agccaattta cgcaacgatg tctccgcagt cgaagaggtg gaagcattaa tgcgtaatat
    16801 tgccgatgcc tggaaagagt ctttactctc cccttcttcg attcaggacc cagtctgatg
    16861 aacaatgcac cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg
    16921 ttacgcctgg caacggaaga acaatgggac gaactcatcg ctagcgaaat ggcgtatgtg
    16981 aatgcggtgc aggagatcgc acacctgact gaagaggttg ccccgtccac tacgatgcag
    17041 gagcagctcc gcccgatgct gcacctgatt ctcgacaacg aaagcaaggt aaagcagtta
    17101 ttacagattc ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg
    17161 gtgttaagtg cctatggcga tcagggcggc tttgtgctgg ctccgcagga taacctcttt
    17221 tgaatcttaa tgagccgatg gctcgcgaat aatccgatta cggctacgct tctaatattc
    17281 cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt
    17341 acccggaagg cggtaagctc tggcctgaac tggccgagcg cgcagacggt tttaatgata
    17401 ttggtatcaa tatggtctgg ttgccgcccg cctataaagg cgcatcgggc ggctattcgg
    17461 tcggctacga ctcctatgat ttatttgatc taggtgagtt tgatcagaaa ggcagcatcc
    17521 ctactaaata tggtgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg
    17581 acattgcggt gctgctggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag
    17641 ctattcgcgt gcagcgtgta aatgctgatg accgtacgca aattgacgaa gaaatcattg
    17701 agtgtgaagg ctggacgcgt tacaccttcc ccgctcgtgc cgggcaatac tcgcagttta
    17761 tctgggattt caaatgtttt agcggtatcg accatatcga acaccctgat gaagatggca
    17821 tttttaaaat tgttaacgac tataccggag aaggctggaa cgatcaggtt gatgatgaac
    17881 tcggtaattt cgattatctg atgggtgaga atatcgattt tcgcaatcat gccgtgacgg
    17941 aagagattaa atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc
    18001 ttgatgcggt caaacatatt ccggcatggt tttataaaga gtggatcgaa cacgtacagg
    18061 aagttgcgcc aaagccgctg tttattgtgg ctgaatactg gtcgcatgaa gtcgataaac
    18121 tgcaaacgta tattgatcag gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga
    18181 tgaaattcca tgaagcatcg cgcatgggtc gcgactacga catgacgcag atcttcactg
    18241 ggacgctggt ggaagccgat ccttttcacg ccgtaaccct cgttgccaac cacgacaccc
    18301 aaccgttgca agccctcgaa gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct
    18361 taattttgtt gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcac
    18421 attacgaaga tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac
    18481 agcttgatga gttaattctc gcccgtcagc gtttcgccca cggtgtacag acattatttt
    18541 tcgaccatcc gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg
    18601 tggtggtcat gtcgaacgga gatgatggcg aaaaaaccat tcatctggga gagaattacg
    18661 gcaataaaac ctggcgtgat tttctgggga accggcaaga gagcgtagtg acagacgaaa
    18721 acggcgaagc gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg
    18781 tgatttaaat ccatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt
    18841 aacggcgctt tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc
    18901 gtcataccat cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt
    18961 acggtaacgt tagtgagatc atcagacatc aatgtcaact tacccggcac ggcaatcccc
    19021 cgctgccact gacggcaatc caaggtatcg ccctctttag tgacaatcag gctgcctatc
    19081 gcttccggac tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag
    19141 tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc
    19201 aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag
    19261 aggattgtgg cattaaagct ctgatgtcgc cagcctttcc aggttcagag agaaatttga
    19321 tctaactatt atttttgtga attattaccc cgaaaataaa tttgcatggt gtatgattcg
    19381 cggctcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca
    19441 aacacgcctg gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc
    19501 tctccactta ctattttggc attactggca ccttttgggc tgtcacgggt gaatttaccc
    19561 gttggggcgg tcagctcctg cagctgtttg gcgtccatgc tgaagagtgg ggttatttta
    19621 aaattatcca tctggaagga tcgccattaa cccgcatcga cggcatgatg atcctcggta
    19681 tgtttggcgg ctgctttgct gctgcgctgt gggccaacaa tgtcaaacta cgcatgccac
    19741 gcagccgtat ccgcattatg caggccatca ttggcggcat tatcgccggt tttggcgcgc
    19801 gtctggcaat gggctgtaac ctggcggcgt tctttaccgg cattcctcag ttctcgctgc
    19861 atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc
    19921 tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga
    19981 cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca
    20041 tgctgggctg ggcgctgctc acggcgatga accaaccaaa actggggctg gcaatgctgt
    20101 ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc
    20161 gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatc ggtatggcgg
    20221 tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacctaaa atcatgtggg
    20281 cgggaccaaa tgcggtaatt ggtggtttac tttttggttt tggcatcgtg ctggcaggcg
    20341 gctgcgaaac cggctggatg taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg
    20401 gtctgggcaa cgtgatcggc tcaacgattc tggcgtatta ctgggatgat ttcgctccgg
    20461 cgctggccac cgactgggac aaaatcaacc tgctgaaaac ttttggtccg atgggaggcc
    20521 tgctggtgac atatttgctg ctgtttgctg cgctgatgtt gattatcggc tgggaaaaac
    20581 gcttcttccg ccgtgaggca ccgcagactg ctaaggagat cgcatgaaaa atatcgttcc
    20641 tgattaccgc cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg caacccttga
    20701 ggcgatgccg caattaaaaa aaggtgagat cctggaagtg gtgagcgact gtccgcagtc
    20761 gatcaataat attccactgg atgcgcgtaa tcacggctac acggtgctgg atattcagca
    20821 agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg ctgtggccca
    20881 cagcgggaac atcgttacct ctgacacata gattttaccc gccccctctc ctattctttt
    20941 cagtatgatc tcaggaggtt gctatgtgtg gacgctttgc ccaatcccaa acgcgtgagg
    21001 attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctacgat cccgaaccca
    21061 ttggcagata caatgtcgcg ccgggaacca aagttcttct gctcagtgaa cgtgatgaac
    21121 accttcatct ggatccggtt ttctggggat atgctcccgg atggtgggat aaaccgccgc
    21181 tgattaacgc ccgcgtagaa actgcctcaa ccagtcgcat gtttaaaccg ctctggcaac
    21241 atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa
    21301 aaagccttat tttatctatc gcgctgacgg acaacctgtt tttatggccg cgataggtag
    21361 cataccgttt gagcgtggtg acgaagccga aggatttttg atcgtcactg cggcggcaga
    21421 tcaaggtctg gtagatattc atgaccgccg cccgctggtt ctgtcgccag aagctgcacg
    21481 ggaatggatg cggcaagaca ttggtggtaa agaagcctca gaaatcgcta ccagaagctg
    21541 tgtgccagca aaccagttca cctggcaccc tgtgtcgcgc acggtgggta atgtcaaaaa
    21601 ccagggcgcg gagttaattc aacctgtttg ttgacatcct ccacgccctg aatgacgagg
    21661 acccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc
    21721 ggcctgactg caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac
    21781 gagctccatt tacatcggcg ttcgctgtat atccacatac ctggcatctg aatttacttt
    21841 gtgacaggcg attttctttc gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg
    21901 ctggcggaac agcaagcacc tgaccgccac gccagagctg cttgtactca agctggcggc
    21961 gcatttcata ccagccctga tccagtatcg aacggtttaa acctgatttt gcccggacat
    22021 tgcgccccgg ctggctgacc gtacccgctg ctgactttga catgttgctg accttcaaat
    22081 cctcaatgac tatcattacg tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt
    22141 ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc tgcttctgcc
    22201 agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc agcttcttct
    22261 ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc gaggcaagtt
    22321 tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga gttgatactt
    22381 cactttctgc ctgaatactg atgtaccact taccgcagga ctggctgaca gtgacatttt
    22441 tcacaacacc cgtgacctga cggctgttcc ggtagcgcat ccagccaagt tttggcagaa
    22501 aaatacggct gttttcctga gcgagcttaa cgccctgcgg atagcggaat gcatcattct
    22561 gtcaccgctt tttgaatcag gggaacgccg agcgcttctg gaaaaagttt ttgcaggcct
    22621 gctcagggtc tttcagtgac tgttgcaatg gctgtgacgg agcatcttta atccattgcg
    22681 tttcagtggc atttttccac tcaaccaatc aggaagccat tttcgcgtaa gggatgtctt
    22741 tgttaccggc cttatgattc tcattctgac gtgccagagt acgactgaaa acgaaacgac
    22801 atgcgcctgc gaagcgcctc atttcacgcg cctgttgacc accgggtctt aaccggaatt
    22861 tgaatacctg tagtcgtttc acagggcaaa ttatactttg gtctgtgaaa aaaactgata
    22921 tccgacgcgg aaggcactgt gttttcctga tgcatgctca ccgggtcttt gttaccaagt
    22981 accgacgcca gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg
    23041 tatgtgctta ttttgaagct gaactggttg aaatggatgg cgaaccagat cacgtccatc
    23101 tgttaattaa ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa
    23161 ggcgtatcgg gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc
    23221 aaagatgttt tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc
    23281 gtcatccgcc aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta
    23341 tatccccgcc ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac
    23401 acctgcatgg acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa
    23461 agtattagag ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa
    23521 aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac
    23581 attaataaat ctaaataaat atcatgcaaa taatttccat ggttattttt aggctattta
    23641 aattaaaaaa ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac
    23701 caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata
    23761 aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
    23821 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
    23881 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
    23941 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
    24001 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
    24061 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
    24121 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
    24181 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
    24241 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
    24301 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
    24361 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
    24421 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
    24481 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
    24541 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
    24601 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
    24661 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
    24721 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
    24781 ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca
    24841 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
    24901 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
    24961 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
    25021 atataagtgg cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata
    25081 aattaccctt tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca
    25141 attaacgtat caattagatt taataacaac gatatccgta cctaacctga tatctaatgt
    25201 cataagcatc cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca
    25261 catagcccaa acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag
    25321 gaagtgagta tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga
    25381 ttgttcaggc tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc
    25441 tgttgctcct taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
    25501 atattaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
    25561 ctggcaatct cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa
    25621 cctcacgtcg tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa
    25681 tgtggaaaca gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg
    25741 ttcggatagc attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag
    25801 taaacaatta acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg
    25861 aatttgcgtt gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa
    25921 ctcggggttc tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata
    25981 aaatttttga aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag
    26041 acctgcttct ggataattca caatataaaa ttcatatcca cagggaaata atattatctg
    26101 attattatca acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat
    26161 acgacataaa gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg
    26221 tatttcttca atctttaggt tagacttatc tcttgccagc atactcttac cctattttat
    26281 ctcataaact ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac
    26341 taacgctccg ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt
    26401 aaactggtaa tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata
    26461 accaaaatca tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc
    26521 tgcatcagtg ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca
    26581 tccactgcct gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc
    26641 aggagcacca gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga
    26701 aaatacatga gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa
    26761 tgctgcggcc tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt
    26821 cataaaaacc tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta
    26881 tgaataaagg cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt
    26941 agccataata tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg
    27001 tgcacttcgt gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat
    27061 gatagctggc aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac
    27121 gataccgact cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg
    27181 agctaataac cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg
    27241 tgtaaacccc tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc
    27301 accaagataa atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt
    27361 actacagttt gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct
    27421 ggcgtggcaa gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa
    27481 tgagttcact cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg
    27541 tggatggcag aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt
    27601 gccccatcca tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt
    27661 tacacaacct acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc
    27721 acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt
    27781 agcgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt
    27841 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc
    27901 ggcagtgatt cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct
    27961 tcaatcccct gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac
    28021 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat
    28081 agaaaaaacg aaagcacaaa taatgggagc gtcaattttt cgagtttgct gacccgagag
    28141 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga
    28201 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
    28261 aaattccatt gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
    28321 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
    28381 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
    28441 ctattgaggt tccggcagat aaagttcacg aactgcacct gcgcctggcg caacaaggtt
    28501 tgcccaaagg cggcgcggtc ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc
    28561 agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg
    28621 aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat
    28681 tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg
    28741 cactcgatga agggcaaatt agcgctattg tacatctggt ttccagcgcg gttgctggtc
    28801 tgccgcctgg aaacgtcacg ctggtggatc agggcgggca tctgttaacc cagtccaata
    28861 ccagcgggcg cgatcttaat gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta
    28921 ttcagcagcg tattgaagcg atcctgtcac ctattgttgg taacggtaat attcacgcct
    28981 aggtcacggc gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta
    29041 acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg
    29101 gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata
    29161 acgcgccaat cagcacgcct ccgacaaatc aaaataaccg ccagcagcag gcgagcacca
    29221 ccagcaatag tgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc
    29281 gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag
    29341 tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga
    29401 agcaaattga agctctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc
    29461 tcaatgttgt taactcgccg ttcaatagca gtgacgaaag cggcggagca ctgccgtttt
    29521 ggcaacagca agtgtttatc gatcagttgc tggctgccgg tcgctggttg ctggttgctg
    29581 gtactgctgg tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc
    29641 gccgaggcgg tgaagactgt gcagcaacag gcgcaggccc acgaggaagt ggaagatgcg
    29701 gtggaagtcc gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg
    29761 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
    29821 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
    29881 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
    29941 ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
    30001 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgctgcact
    30061 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg
    30121 tgccgccagc ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac
    30181 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
    30241 tatcgccacc attctggtgc atctgaagcg cgcctaggcc gccgatattc tggcgttgtt
    30301 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
    30361 agccgcgctg gccgaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
    30421 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca
    30481 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
    30541 catcgacgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg
    30601 tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc
    30661 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
    30721 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct
    30781 gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg attggcagcg gcgaggatac
    30841 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg
    30901 cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc
    30961 ttgagcagca actggcgcaa ctgcaaatgc aggcccacga gcagggttat caggcgggga
    31021 ttgccgaagg tcgccagcaa ggtcacgaac agggctatca ggaaggactg gcccaggggc
    31081 tggaacaagg tctggcagag gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc
    31141 aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgata gcttcgcgcc
    31201 tgatgcagat ggcgctggag gcagcacgtc aggtgatcgg tcagacacca acggtggata
    31261 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggga
    31321 aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa
    31381 ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccaccct ggcggatgta
    31441 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
    31501 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
    31561 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
    31621 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
    31681 ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
    31741 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg
    31801 cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca agcagttgcc
    31861 gctcggtccg gcgttattag gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct
    31921 gcccttcccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
    31981 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
    32041 cgtcggacgc gggcagcgta tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct
    32101 gctggggatg atggcacgct acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
    32161 acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
    32221 ctcagtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc
    32281 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
    32341 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
    32401 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
    32461 tgccggtaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
    32521 aggcgatgac cagcaggacc ccattgctga ctccgcgcgg gcaatcctcg acggtcacat
    32581 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
    32641 gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
    32701 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
    32761 caaaggcagc gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta
    32821 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
    32881 tattttcccg acagtgtcat aacccaggag ataacggcag atggcggaag aacagctcaa
    32941 aatgttgatc gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg
    33001 gatgaccagc aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat
    33061 tactcagcat cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag
    33121 ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac
    33181 ggcggcactg cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg
    33241 cgccgccatg aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac
    33301 accaccacat tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg
    33361 agcgaagcat tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtagca
    33421 acagataagc ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa
    33481 caagcggatt tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc
    33541 acatcaacac cgttaaccac cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat
    33601 acgacaaaag acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc
    33661 ctttttgcga tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc
    33721 gtgttaccgg cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca
    33781 gcacagcctg atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa
    33841 gcccagagta aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg
    33901 ctaatcactc cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgta
    33961 ccgctgggtt ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc
    34021 caggggcaac aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt
    34081 tccctcaaag tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta
    34141 cgcgccgccc tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt
    34201 cagttagggc aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc
    34261 cagcaacagc aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat
    34321 acgcttccgg ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt
    34381 ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca
    34441 agacgcagga taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg
    34501 attacgcgat aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta
    34561 ccctcgcggc ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg
    34621 acgacaaagc gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta
    34681 cggtcaattt gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag
    34741 atgaagcaac ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt
    34801 tactgttttc acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga
    34861 ttgccgagat taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg
    34921 tcaccgacgt gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt
    34981 tctcaagctg aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca
    35041 gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt
    35101 gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt
    35161 atggggctgt tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt
    35221 cagccgtacc atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac
    35281 ctgaaaccgc tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc
    35341 gtggataacc tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt
    35401 acccatactg aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc
    35461 gacgcctgga aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg
    35521 gaatttacca atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg
    35581 gagattggca acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg
    35641 ctacgggaat tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg
    35701 cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc
    35761 gatatctcgc tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata
    35821 gaaaaacccg atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac
    35881 ggtactctca acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat
    35941 tctctgaacg aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg
    36001 gcgcaatgga cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa
    36061 gcgctgccga cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg
    36121 atatcgacct gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga
    36181 tgaccatcaa agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg
    36241 cgggcgaacc gctggatatt ctgatcaacg gttatttaat cgcccagggc gaagtggtgg
    36301 ttgttgccga taaatatggc gtgcggatca ccgatatcat taccccgtcc gagcggatgc
    36361 gccgcctgag ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc
    36421 tgctgagcca ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc
    36481 tgctgcctgg ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct
    36541 gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga
    36601 agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc
    36661 cccttcagca ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt
    36721 gcttaagcgt ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc
    36781 tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct
    36841 ggcggtggac aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg
    36901 ttcattccag cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt
    36961 ttattgcgta acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg
    37021 gcactgtttt tgaccttttt tattatgtcg ccagtgatca acaaaattta tgtcgatgcg
    37081 taccagccat tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag
    37141 ccgctgcgtg agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga
    37201 ctggcgaata ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg
    37261 gcctacgtga ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct
    37321 tttttgatta tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt
    37381 ccccccgcca ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg
    37441 caattgctgg tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct
    37501 gaatcggtca tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg
    37561 ctgttgctgg tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag
    37621 attaacgaaa tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt
    37681 attgccggac cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac
    37741 ctgccgtata tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt
    37801 cctggttaag cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc
    37861 caattctgag cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca
    37921 cgttcgccat tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc
    37981 tgtggctggc cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg
    38041 cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg
    38101 cgacatttgt cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata
    38161 tgctggcatt actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg
    38221 tcgatacctt tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg
    38281 cacccaccaa agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca
    38341 ttactctgtt gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat
    38401 tatccatttt tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg
    38461 cattaatgcc gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc
    38521 tggctgatat tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc
    38581 taaggattat ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat
    38641 atttgtttta agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt
    38701 atacaacact ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt
    38761 gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg
    38821 cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact
    38881 aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga
    38941 aaccgttgat gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa
    39001 tgaggactgt ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca
    39061 acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata
    39121 tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga
    39181 cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc
    39241 gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg
    39301 aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg
    39361 taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact
    39421 gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg
    39481 tttcccacca gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga
    39541 tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcgc aaccaggtac
    39601 attgtgcctt cactaaactc ctcaactgcc agtaccactc caggacgacg cggaccgcca
    39661 tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc
    39721 agtgtagaac aatttcgttt ttctggcagc gccaagcgcg cgcgagtgct gattttctcg
    39781 acggtctata cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac
    39841 ggttgcgagg aaagatgatg aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg
    39901 tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc
    39961 atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc
    40021 gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc
    40081 gttgaatgca caataaaaaa atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc
    40141 aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc
    40201 aatttttttc gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata
    40261 gaaatgtgac gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa
    40321 atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga
    40381 ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg
    40441 acagtcatag ttatgactgg caaccggcag ccccctggct cagccgttta cacgaagcca
    40501 atattcccgt cattctctgt agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa
    40561 tgttggggct acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg
    40621 aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa
    40681 tctgccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg
    40741 atgtcgacga tgcaaccatt gccgaatgga cgggattaag ccgtagctag gcggcgctga
    40801 cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac
    40861 aatttatcgc tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc
    40921 acgtactgga tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc
    40981 aacaattgtc aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc
    41041 ctttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac
    41101 atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct
    41161 ggcgtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc
    41221 gattgcgccc ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt
    41281 caaaatcata atcaccggtt tcctcgctgc tacttacccc cagcgaggca ctgatgcgta
    41341 tcgttgtact cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg
    41401 cgacctccgc agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac
    41461 cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag
    41521 aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa
    41581 gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac
    41641 gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct
    41701 gccactgcaa cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc
    41761 aggagaggag taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac
    41821 tgccgaaatc gccgcgcacg ccttcgctta gcgtatggac gcgcgccagc acaccgtcga
    41881 aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt
    41941 catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg
    42001 gatggtcagg attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac
    42061 catcgaggtt tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac
    42121 tcatccccag cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct
    42181 gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac
    42241 gattttctcg ttgcgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg
    42301 ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga
    42361 catacatcgc ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa
    42421 cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggaaattcc
    42481 cctcgctgac taatgcatcc gatacgccat tgactggcag ggtgcgccgt cggttgagtt
    42541 cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgcagag
    42601 aggtgaaatc cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt
    42661 tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac
    42721 gctagctgga gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg
    42781 aaaaaagcaa taccacgata aagcagagat taacgacatg accgggcccc aggcggcgtg
    42841 cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc
    42901 ccaacctgct tttttatgat tctggcataa cccggcaata gcgtaccgca aaacagcgat
    42961 aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca ttggaacacc
    43021 aggccggatg tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg
    43081 actcccctcc cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc
    43141 gtcataccaa cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc
    43201 gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg
    43261 agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta
    43321 ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact cccagcacca cgatgccgcc
    43381 gatgataaat ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact
    43441 ttgctgcccg gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat
    43501 cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca
    43561 aggcgcgata atcaataacc ctttacctaa tgcctgcatc agcgcgctag atttttccgc
    43621 cagccaatac cctaggtcat caatcttaac gataacccct accagaccgt agacgcccac
    43681 ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc
    43741 cacaatcccc agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc
    43801 ccctttaatt ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg
    43861 ctgctggctc tgcgctggat cttctttatg tttacgcgcc tccagcatat gcagcacttt
    43921 ctctacccct tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca
    43981 cgggataaac gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga
    44041 tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg
    44101 ttgcgcattg agcgataaat catcccctaa tacaccggcg gttttcttcg ccgccagttt
    44161 gcccatcaca gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact
    44221 actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa
    44281 aaacggcagc tctgatactt gcttcacagg tcaacattta tttaacaatc aagtgcaatt
    44341 atatctctcg ccagcagaat gattttcacg tttactatga accacctttt tatttccgct
    44401 gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta
    44461 tatcatttgg ggctcaacct attttgtcat tcggattggc gtgggaagct ggcctccgtt
    44521 aatgatggcg ggcgttcgat tccaggcagc cggtatttta ttgctggcat ttttgctact
    44581 gcgcggacac aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt
    44641 attgctggct gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg
    44701 catcgccgcc gtagtgattg caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt
    44761 tggcattaaa acgcgcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat
    44821 catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat
    44881 cggctcgatt agctgggcgt ttggctcagt ttatggctcg cgcattacct tacctgtagg
    44941 gatgatggcg ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat
    45001 gattgcgggt gaaaaactga cggcgctccc ttccctgtca ggcttccttg cggtcggcta
    45061 tctggcgctg tttggttcga ttatcgccat taacgcttat atgtatttaa tccgtaatgt
    45121 cagtccggct ctcgccacca gctacgccta cgttaacccg gtggtcgcgg tcttgctggg
    45181 tacgggactg ggtggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt
    45241 tttcgcggtg gtactggtca cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc
    45301 gccagttatt caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc
    45361 tggcaccttc gccgcagatc cactcttcca gacgctcggt aagcgccgca tccgtcagct
    45421 tctcgcgcct acgtaacgca cactcccaga caatcagtac gcgccagccg agctcctgca
    45481 agcgactgat atcgcggcga tcgcgttcaa catttttacc tatcttctcc agccagaact
    45541 cagttcgagt cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt
    45601 gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg
    45661 cgtcctgaac gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa
    45721 tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa
    45781 cgtcgaccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca
    45841 agcagttttg ccaccgcggc aaagaccggc acgaccaccg agttgccgaa ctggcgatag
    45901 gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt
    45961 aagcgcgcgc attcacgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg
    46021 tcaaagtctt tctcacccgt ggccatatcc caaccgcgat cgattaaaat ttccgcgcca
    46081 tctttgtaat aacgagcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc
    46141 attccataac cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagaggtac
    46201 ttccacagca ccggcgtcag gatatatttc gcctcaacca tcgggtccag cagctgcgcc
    46261 agcgtcactc gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc
    46321 agattcagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa
    46381 tgtttaccat cgatgatttt cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca
    46441 tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactc
    46501 ttcaggtttt tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc
    46561 accacatcaa agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc
    46621 gagttctttt tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa
    46681 acatcgtgtt caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct
    46741 tcttgatgac tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg
    46801 caataatggt tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg
    46861 cactgtccac caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg
    46921 ataaagcgaa acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga
    46981 tgggcaaact ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt
    47041 aaaattgccg cgctccagtg attctcaccc acaccattaa gctgcgccac cagcatttta
    47101 acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat
    47161 gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca
    47221 ggttaacata attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct
    47281 atcaccttgt gatcgatacc ttcattctcc cccgccagtt cggcaccgag cttcgccatc
    47341 aaacagcgcc aagctcaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc
    47401 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    47461 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg
    47521 aaattgcacg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    47581 acgctgccgt tgcggatgat ttttcgccag gctcacgata tcgtgaaaat aacaggcggt
    47641 taaaatcacc agcatatcga catcgtcatc tgccgccagt ttctgtgccg tagcccagac
    47701 acggcgaaaa tgacacaggt catgcgccgc gtcctgatgt tgatgatggt ttttcaacca
    47761 gttctcgaac tgtgcctgcc agtgttgtaa ctccatacgc gctccgttgc caaagagtcg
    47821 caaccttagc agtttttatt catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca
    47881 tgcaatgccg cctaacaagg caccaaccag ggccataaac agaaatccga ccaatgctcc
    47941 taaccacttc cccgcggtag atcctaaatc actttcaaat cccgacacgg gggcatcggg
    48001 gatccggctc atcacccaga tgtatcgcgc cattgcccag acgaaatagc cacaccatgt
    48061 atagaaagcc c
//
LOCUS       DAECOD010000020        47797 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.020, whole genome shotgun sequence.
ACCESSION   DAECOD010000020 DAECOD010000000
VERSION     DAECOD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.020"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="KKO15_001158"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="KKO15_001158"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBD6360021.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="KKO15_001159"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="KKO15_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBD6360022.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KKO15_001160"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KKO15_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBD6360023.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KKO15_001161"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KKO15_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBD6360024.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KKO15_001162"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KKO15_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBD6360025.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="KKO15_001163"
     CDS             5377..5835
                     /locus_tag="KKO15_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBD6360026.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="KKO15_001164"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="KKO15_001164"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBD6360027.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="KKO15_001165"
     tRNA            6887..6971
                     /locus_tag="KKO15_001165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="KKO15_001166"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="KKO15_001166"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KKO15_001167"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KKO15_001167"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD6360028.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KKO15_001168"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KKO15_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD6360029.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KKO15_001169"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KKO15_001169"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD6360030.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="KKO15_001170"
     CDS             complement(10252..13995)
                     /locus_tag="KKO15_001170"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBD6360031.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KKO15_001171"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KKO15_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBD6360032.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="KKO15_001172"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="KKO15_001172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBD6360033.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLTLDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="KKO15_001173"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="KKO15_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBD6360034.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KKO15_001174"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KKO15_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBD6360035.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="KKO15_001175"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="KKO15_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBD6360036.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KKO15_001176"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KKO15_001176"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBD6360037.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KKO15_001177"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KKO15_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBD6360038.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="KKO15_001178"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="KKO15_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBD6360039.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="KKO15_001179"
     CDS             23392..23499
                     /locus_tag="KKO15_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6360040.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KKO15_001180"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KKO15_001180"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBD6360041.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="KKO15_001181"
     CDS             complement(24538..25347)
                     /locus_tag="KKO15_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBD6360042.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="KKO15_001182"
     CDS             complement(25351..25743)
                     /locus_tag="KKO15_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBD6360043.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KKO15_001183"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KKO15_001183"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD6360044.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KKO15_001184"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KKO15_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBD6360045.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KKO15_001185"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KKO15_001185"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBD6360046.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="KKO15_001186"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="KKO15_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBD6360047.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="KKO15_001187"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="KKO15_001187"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBD6360048.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="KKO15_001188"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="KKO15_001188"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBD6360049.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="KKO15_001189"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="KKO15_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBD6360050.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KKO15_001190"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KKO15_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBD6360051.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="KKO15_001191"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="KKO15_001191"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD6360052.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="KKO15_001192"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="KKO15_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBD6360053.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="KKO15_001193"
     CDS             36790..39657
                     /locus_tag="KKO15_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6360054.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="KKO15_001194"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="KKO15_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBD6360055.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="KKO15_001195"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="KKO15_001195"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBD6360056.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="KKO15_001196"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="KKO15_001196"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBD6360057.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="KKO15_001197"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="KKO15_001197"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBD6360058.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="KKO15_001198"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="KKO15_001198"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD6360059.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="KKO15_001199"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="KKO15_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11993 a        12313 c        11702 g        11789 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcagcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagaggcagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcattcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctgacgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtcgg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga attagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacagtta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaagtcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgc
//
LOCUS       DAECOD010000021        47400 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.021, whole genome shotgun sequence.
ACCESSION   DAECOD010000021 DAECOD010000000
VERSION     DAECOD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.021"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KKO15_001200"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KKO15_001200"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBD6360060.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="KKO15_001201"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="KKO15_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBD6360061.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="KKO15_001202"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="KKO15_001202"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBD6360062.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="KKO15_001203"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="KKO15_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBD6360063.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="KKO15_001204"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="KKO15_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBD6360064.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="KKO15_001205"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="KKO15_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBD6360065.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="KKO15_001206"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="KKO15_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBD6360066.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="KKO15_001207"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="KKO15_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBD6360067.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="KKO15_001208"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="KKO15_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBD6360068.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="KKO15_001209"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="KKO15_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBD6360069.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="KKO15_001210"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="KKO15_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBD6360070.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="KKO15_001211"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="KKO15_001211"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBD6360071.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="KKO15_001212"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="KKO15_001212"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBD6360072.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="KKO15_001213"
     CDS             11231..12085
                     /locus_tag="KKO15_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD6360073.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="KKO15_001214"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="KKO15_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBD6360074.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="KKO15_001215"
     CDS             complement(13548..14687)
                     /locus_tag="KKO15_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBD6360075.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="KKO15_001216"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="KKO15_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBD6360076.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="KKO15_001217"
     CDS             15873..17234
                     /locus_tag="KKO15_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBD6360077.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="KKO15_001218"
     CDS             17231..17749
                     /locus_tag="KKO15_001218"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBD6360078.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="KKO15_001219"
     CDS             17749..18069
                     /locus_tag="KKO15_001219"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBD6360079.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="KKO15_001220"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="KKO15_001220"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBD6360080.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20090
                     /locus_tag="KKO15_001221"
     CDS             18888..20090
                     /locus_tag="KKO15_001221"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBD6360081.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20187..20609
                     /locus_tag="KKO15_001222"
     CDS             20187..20609
                     /locus_tag="KKO15_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD6360082.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20657..21529)
                     /locus_tag="KKO15_001223"
     CDS             complement(20657..21529)
                     /locus_tag="KKO15_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD6360083.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21541..22635)
                     /locus_tag="KKO15_001224"
     CDS             complement(21541..22635)
                     /locus_tag="KKO15_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6360084.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22668..23666)
                     /locus_tag="KKO15_001225"
     CDS             complement(22668..23666)
                     /locus_tag="KKO15_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6360085.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23691..25202)
                     /locus_tag="KKO15_001226"
     CDS             complement(23691..25202)
                     /locus_tag="KKO15_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD6360086.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25225..26208)
                     /locus_tag="KKO15_001227"
     CDS             complement(25225..26208)
                     /locus_tag="KKO15_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD6360087.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26305..29406)
                     /locus_tag="KKO15_001228"
     CDS             complement(26305..29406)
                     /locus_tag="KKO15_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBD6360088.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29413..29586)
                     /locus_tag="KKO15_001229"
     CDS             complement(29413..29586)
                     /locus_tag="KKO15_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360089.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29704..30897
                     /locus_tag="KKO15_001230"
     CDS             29704..30897
                     /locus_tag="KKO15_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBD6360090.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31095..32348)
                     /gene="glyA"
                     /locus_tag="KKO15_001231"
     CDS             complement(31095..32348)
                     /gene="glyA"
                     /locus_tag="KKO15_001231"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBD6360091.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32675..33865
                     /gene="hmpA"
                     /locus_tag="KKO15_001232"
     CDS             32675..33865
                     /gene="hmpA"
                     /locus_tag="KKO15_001232"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBD6360092.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGLVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33910..34248)
                     /gene="glnB"
                     /locus_tag="KKO15_001233"
     CDS             complement(33910..34248)
                     /gene="glnB"
                     /locus_tag="KKO15_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBD6360093.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34309..35643)
                     /gene="glrR"
                     /locus_tag="KKO15_001234"
     CDS             complement(34309..35643)
                     /gene="glrR"
                     /locus_tag="KKO15_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBD6360094.1"
                     /translation="MSHKPAHLLLVDDDLGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35633..36346)
                     /gene="qseG"
                     /locus_tag="KKO15_001235"
     CDS             complement(35633..36346)
                     /gene="qseG"
                     /locus_tag="KKO15_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBD6360095.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36511..37938)
                     /gene="qseE"
                     /locus_tag="KKO15_001236"
     CDS             complement(36511..37938)
                     /gene="qseE"
                     /locus_tag="KKO15_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBD6360096.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38514..42401)
                     /gene="purL"
                     /locus_tag="KKO15_001237"
                     /gene_synonym="purI"
     CDS             complement(38514..42401)
                     /gene="purL"
                     /locus_tag="KKO15_001237"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBD6360097.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42660..44216
                     /gene="mltF"
                     /locus_tag="KKO15_001238"
     CDS             42660..44216
                     /gene="mltF"
                     /locus_tag="KKO15_001238"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBD6360098.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44213..44749)
                     /gene="tadA"
                     /locus_tag="KKO15_001239"
     CDS             complement(44213..44749)
                     /gene="tadA"
                     /locus_tag="KKO15_001239"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBD6360099.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44774..45409)
                     /gene="yfhb"
                     /locus_tag="KKO15_001240"
     CDS             complement(44774..45409)
                     /gene="yfhb"
                     /locus_tag="KKO15_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBD6360100.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45618..46466
                     /locus_tag="KKO15_001241"
     CDS             45618..46466
                     /locus_tag="KKO15_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBD6360101.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46805..47359)
                     /gene="pinE"
                     /locus_tag="KKO15_001242"
                     /pseudo
     CDS             complement(46805..47359)
                     /gene="pinE"
                     /locus_tag="KKO15_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11303 a        12980 c        11917 g        11200 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca ctcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcattgcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac
    19441 caccgcccgt gcaacgctat cttttacagc gccatcagca ggctggtgtg cgcattctgc
    19501 tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg
    19561 gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac
    19621 tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca
    19681 cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg
    19741 cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg
    19801 caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca
    19861 gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc
    19921 cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca
    19981 cgctgaatca gggccgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt
    20041 ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa tcaggataat
    20101 tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat
    20161 cacattatct tgcaaaggga ttggttatga acacactacg ttattttgat tttggagctg
    20221 cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc attatttttg
    20281 gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg ttgggcgcgc
    20341 caatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc gcgatattaa
    20401 tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac acgctgggta
    20461 cggcggtgat tggtcaccat tactgggata tgaccggcga tgcggttggg ccaaatatga
    20521 ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca attaccggac
    20581 cgggggcaat ttctctcgat cggcgttagg aaagatgccg gatgcggcgt gaacgcctta
    20641 tccggcattt tataaattac agccactcta cccgcaacga catctctgag gttgttgatt
    20701 cccctggtgc tagcgcaatg agatcacccc cttccgggcg atgatgatca tccggcgcat
    20761 ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg
    20821 aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt
    20881 cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg ttcacccact
    20941 gacgcggcaa cggcgcgagc tggttaaaat ccagttcctg cggtagctgc tcgcaaaact
    21001 cacccgccag ccactgctcc cgttccagcc agtaaccgct cgcctgcgcc tgaatccgcg
    21061 tttgtggtga caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc
    21121 cttgattggt gacagagagc gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac
    21181 ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa ctatcatcgc
    21241 tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc
    21301 actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacgg ttgccgctca
    21361 cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca ccgctttttt
    21421 taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt
    21481 cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc cgctaactcc
    21541 ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca gcacataggc
    21601 atcacctgcc tgttctagcg agaagcgatg ggtaatggcg ttacgcggcc acagcttcca
    21661 gtcagtcaaa tcgtgggcgc atttttccat atggaacaga ctggtcaccc aggagccgat
    21721 aatccgccgt tgatggtgca tcagatcagc gctgacctcg aattccactt ttccggtttc
    21781 accaatgtaa accacccgcc cccagtcagc agtggattgc agtgccagca agcgacctgc
    21841 ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt cagcaattat
    21901 ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc atccccccta actgttttgc
    21961 catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac cgcgaccttt
    22021 cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca cgttatcgct
    22081 gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc aactgataaa
    22141 cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt cttccgccag
    22201 cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt
    22261 acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata
    22321 caccagcacg cggtcgccct ctttaaaatg gcggcagcct tgccccatcg ccacaatctg
    22381 cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg gtgccgccgc
    22441 cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc cggaggattt
    22501 cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc gcagatcgac
    22561 ggttgaattt cctagtaaat aagcagccag catcgttttc ataaatccct cattaacaat
    22621 acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct
    22681 ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat gatgacgccg
    22741 cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt accgattagc
    22801 gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc
    22861 gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg
    22921 ttaccggaac cgaggcgtgc cgccaacaaa atgccggtca ccgccgctaa taatcccgaa
    22981 agggtaaaga taagaatgcg cactcgacga acgttaatac cgcacaactg cgccgccgtg
    23041 gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg tttttcggct aatgaacaca
    23101 aacagcgcaa acaacaccat catgatcagc gcggatactg gcacaccgag aaattgtccg
    23161 cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt ggtcataaac
    23221 agccccattc cgcgcagagc gctccacagc cccagtgtgg caacgaaact tggtacgtta
    23281 aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag caacaccagc
    23341 agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca caccgagaca
    23401 aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat cagcgtcatc
    23461 gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg gttattcaat
    23521 gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat caccaccagc
    23581 aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct cttgccctgc
    23641 ggcaatggta atgacgaagc atacatcttc aggttcctcg gtaaaattca tcagtgcaca
    23701 gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac
    23761 gtgccgtgct gtaataacag gatgcggtca cacaccagcg gcaattcctc cacttcactg
    23821 gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg gtaaatctgc
    23881 tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg
    23941 gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag cgtgccgatg
    24001 ggtgtttcgc tactggcagc cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg
    24061 cggatggtgg accattgcag cacaccgttg gtgctgattt tttgccgatt ggtcagcact
    24121 gtattttcgt caacgcctag ccagggaatg atccccgctt ctttgcggtt ttctggcgta
    24181 tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcatgatttt ctcgccgttg
    24241 ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag caattcactg
    24301 cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cacgacgtag cgtaaagctg
    24361 atatcctcca gcttgggctt atggcgtaac gcacggactt ccagtacggc ctgatccaca
    24421 atttcctgag gggcaaccgg cgcaatatca acgtgatcac gccctagcat tagcgacaca
    24481 atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc atcgcgcata
    24541 acggtggcat aggaggcaat gcggcggatt tcttccatcc ggtggctgac ataaatcacc
    24601 gccacgccca gtgccgacat ctttttcacc gcactgatca ccagttcaac ttctgcactg
    24661 gcaagcgagc tggtgggttc atcaagaatg accacgcgcg gctcgccctt catcacccgt
    24721 gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg ttcaggacta
    24781 acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg caggtaatca
    24841 atcatgccgt tgcggcgagg ccattgacca aggcagaggt tttctgccac cgtcagccct
    24901 tccaccagac ttaattcctg ataaaccgca cgaaccccca gttcagcggc acggcgagtc
    24961 aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc gctatccggg
    25021 cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt tttacctaac
    25081 agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc aacgacgccg
    25141 gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt tgccgtgaac
    25201 atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg ccagccactg
    25261 tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc
    25321 ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc
    25381 ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag caatttcggt
    25441 tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt
    25501 taccgcgccg agtgtcgcac cgcccgactc ccccataatg gcgttgagat ccggcgtgga
    25561 gataatcagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctggttagc
    25621 gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg
    25681 cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt caattttatt
    25741 ggcaataaaa taatcggcgg cagcgttacc cagtttttta ccaaattcca gtggatcgcc
    25801 gaccagatac gccgagacat atttatcgac acccttttga ttaatacagg tgttgtagca
    25861 aatcaccgga atacccgctt cactggcgcg acgaacggta cggctactgc cattttcaga
    25921 cactgccgat aaaataatgg catcgacatt acgcgccacg agggtatcaa caaaggtact
    25981 ttctttcgaa atatccccct gggcgttagt ttcaattaac tgtacctgta ctgaagaatc
    26041 ttttgccgca tcctgaacgc cctgacgcac tccagcgtaa tatccctggg tatcaaggta
    26101 tattgcacca atggtcattt ctttatccgc agccctggca aatagtgcgc tacctaatag
    26161 cgtagccatt aataatagat tacgggttgt tctcattttt ttaggcatag tgcctcctgt
    26221 agggttttta ttaacaacgg cttattctaa ttattttgtg atgagcggca gcgcgtgccg
    26281 ctcaaatatt acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt
    26341 ggtgaataat gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga
    26401 ctcatattgc gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt
    26461 ttctttatgc tgctgttgta aatcgccata cagcgataac aggtcaggtt gtgaaacagc
    26521 aaagaaatcc gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact
    26581 aaacagttgc tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa
    26641 gagataatca accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt
    26701 cgccgccaga cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat
    26761 cgcctgccag aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt
    26821 ctccggataa tacagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc
    26881 aagatgctgc caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc
    26941 cccgccttcc cacgggtgga atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg
    27001 acccgtgaga tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat
    27061 ttccagatta ttttccagac gtgccagtcg tttctccggt gtggctccac ttaacttatc
    27121 cagcaaatcc cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc
    27181 aagataacgc gcggccagct cataatcgtg tttcttattc cacgcatgga tcgctaaccc
    27241 gcgccagccg tcggcaaact ccggcgacat ctctacgcaa cgttgccata aggcaatggc
    27301 tttgttgtag ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca
    27361 ctcttcaata ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg
    27421 cgggaagaca tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct
    27481 ggcttgcagg taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc
    27541 cagggtgggc attccccagt taatcagttg cccggcggtc atcagagcgt taacgtcgcg
    27601 tccctgacac agaccgcgcc actgcgcgag ggctgattcg ctacgaccat cgaaccagtt
    27661 cagccaccac agagtggcgt tcagcggata atcacgcagc agtttctcgc gctgcaaacg
    27721 cgcgttgtcc tgacgaccac ttaacaccag cagcagatta tgcaagcaaa gcacttcctg
    27781 attggttggg caggcacgaa gactttgttg gcaaaaatcc agacccgcgt cgaagttatc
    27841 attacgcgcc gccagtcgtg ccagaccata atagccgcct gctttactat tgccgctcca
    27901 gaccgcacgc cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact
    27961 ggcgcgaatc aaacttgctt gtccacactg cgggtttttg ttcagcgcat gtgcgcgttt
    28021 cagcgcctga ctggcatacg ccaccgcttg cgggaaatct gcgcggttat attccagcat
    28081 cgccagcgcc aggttacagc gataatccag cgggtccagc gccacgccgc gcaggtagta
    28141 atcgaacggt gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc
    28201 ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccggcaacgg
    28261 caactcctgc ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag
    28321 ttcaatggtc agtctgtcag gattgatacc gtgcaacacg ccctggatgg cggtcgctgg
    28381 tgtcagggcc acggcatcat cgagcaacgc gttgcatttg ccgatttcgc ggatcgccag
    28441 acggtatccg ttcaacggag agatggcata cagcccccac tcaatccccc gctcactacg
    28501 ctggagtttt atcaccgcat cgcgggaggc attttgcacc atgcccagag aatgataagg
    28561 caggaaatac tgctcgaaac gcttctcttc gtaagcatca agccaggtaa aatcaggctg
    28621 gttatcggca aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact
    28681 cttatcccac gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc
    28741 aatatggtgg ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac
    28801 aaaatcgtac tgcgattttt cggccatata tgaggttgga acaggcacat ttttatagcg
    28861 agaaatgtcc actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa
    28921 agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct
    28981 ctgatgccct tcaccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt
    29041 ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc gcccggtcag ggcgcagggt
    29101 gaaacctgtc atcacctgta aaccatgcat cggctccgtt tcgccgaccc acaccgtctg
    29161 tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg
    29221 gtgttgcggc cagttaaact caatcccgcc ggagatccac ggtcccagca tccccaccag
    29281 cgcaggttta atgacttcat tgtgatagac aaaatcgcgc tgtttcactt tatcccatgc
    29341 gcgatgcacc cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag
    29401 ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta
    29461 gggataaacc gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat
    29521 atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg
    29581 agtcatggtg ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat
    29641 tgaacaatct ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg
    29701 aagatgagag cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg
    29761 gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt
    29821 tcgattccgg cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat
    29881 attgacgatg aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa
    29941 ggtgactgga cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat
    30001 gcttgtttaa gtccgaaagg cgaatttgag tatttacaga ttgatgtacc gacgccgcag
    30061 gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc
    30121 accatcaatc tggcgctggc aatccacggg caggttgatc cggtgaccgg cgtatcgcaa
    30181 accatgccgc aagcgccgtg gacaacgcca gttgaggtga agtatctgct ggaagagaag
    30241 ctgggcattc gggtgctggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg
    30301 caaaataatt cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg
    30361 tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acagattggt
    30421 cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg cagcctggaa
    30481 accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg
    30541 gttaatactc agcttgagcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag
    30601 agtggagaac cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt
    30661 ctgtataact tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt
    30721 gcctttggtg agaactggct taatactatt attcgccaga caggatttaa cccgttcgac
    30781 cgcgacgaag gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca
    30841 caacaggtgc tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg
    30901 cgcgataacg tagaaaggct tcccgaagga agccttgatg atcataaacg aaaaattgcc
    30961 tgatgcgcta cgcttatcag gcctacacgg agattgcaat atattgaatt tgcaagattt
    31021 tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa caacgagcac attgtcagca
    31081 aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag aactttacct
    31141 ttgatgcgct cgataacggc ttcatcattg atgctgtcca gcacgtcaca catccagcca
    31201 gccagttctt tcgcttcggc ttctttaaag ccgcgacggg taatcgccgg agtacctaca
    31261 cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt tttgttgacg
    31321 gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt tttatcaacc
    31381 agatcaacca ggaacaggtg gttatcagtg ccgccggaaa ccactttgta gccgcgctcg
    31441 aggaacactt ctaccatcgc tttagcgttt ttcgcgacct gctgctggta agttttgaac
    31501 tcaggctcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg catcaacgga
    31561 ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc ttcgctacca
    31621 cctttcgcca agatcaggcc gccgcgcgga cccgccaggg ttttgtgagt ggtggtagta
    31681 acaacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag gcccgcaacg
    31741 tgcgccatat caacgaacag ataagcaccg atgctgtcag cgatttcacg cattttcgcc
    31801 cagtcaacca cgccggaata tgcagagaaa ccaccgataa tcattttcgg cttgtgttct
    31861 ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg tagcatcgat accgtaagga
    31921 acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag gtgaccgcca
    31981 tgcgccaggt tcatacccag aacggtatca cctggttcca gcagcgcggt gtagaccgca
    32041 aagttagcct gggagccgga gtgcggctgg acgttagcgt agtcagcgcc gaacagttct
    32101 ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc gccgtagtag
    32161 cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg cgcctgcatt
    32221 acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc ctgacgtact
    32281 ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt catttcacgc
    32341 tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt ataggcggtc
    32401 ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt cttgacaaag
    32461 gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa agagcttgca
    32521 accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat cacattcttt
    32581 ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga tgcatttgag
    32641 atacatcaat taagatgcaa aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac
    32701 agtaaaagcc accatccctt tactggtgga aacggggcca aagttaaccg cccatttcta
    32761 cgaccgtatg tttactcata acccagaact caaagaaatt tttaacatga gtaaccagcg
    32821 taatggcgat caacgtgaag ccctgtttaa cgctattgcc gcctacgcca gtaatattga
    32881 aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg cagaagcaca ccagcttcca
    32941 gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat
    33001 gttcagcccg gggcaggaag tgctggacgc gtggggtaaa gcctatggtg tactggctaa
    33061 tgtatttatc aatcgcgagg cggaaatcta taacgaaaac gccagcaaag ccggtggttg
    33121 ggaaggtact cgcgatttcc gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag
    33181 cttcgaactg gagccggtcg acggtggcgc agtggcagaa taccgtccgg ggcaatatct
    33241 cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa attcgtcagt actctttgac
    33301 tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa cgcgaagagg gtgggctggt
    33361 atccaactgg ttgcacaatc acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc
    33421 aggtgatttc tttatggctg tcgcagatga cacaccagtg acgttaatct ctaccggtgt
    33481 tggtcaaacg ccaatgctgg caatgctcga cacgctggca aaagcaggcc acacagcaca
    33541 agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac gcctttgctg atgaagttaa
    33601 ggaactgggg cagtcactgc cgcgctttac cgcgcacacc tggtatcgtc agccgagcga
    33661 agccgatcgc gctaaaggtc agtttgatag cgaaggtctg atggatttga gcaaactgga
    33721 aggtgcgttc agcgatccga caatgcagtt ctatctctgc ggcccggttg gcttcatgca
    33781 gtttaccgcg aaacagttag tggatctggg cgtgaagcag gaaaacattc attacgaatg
    33841 ctttggcccg cataaggtgc tgtaatttga tgttgccgga tggaaacatc cggcaaccct
    33901 tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta cggatgcgaa tgacccgagc
    33961 cacgtcaaag acgaagattt taccgtcacc gattttgccg gtttgcgccg tgcgaataat
    34021 ggtatcgaca caggtatcga caatgtcgtc cggtacgaca atctcaattt tcactttcgg
    34081 cagaaaatcc accatatact ccgcgccgcg gtacagctcg gtatggcctt tctggcgacc
    34141 aaagcctttc acttcggtca ccgtcatgcc ggtaataccg acttcggcca gtgcttcgcg
    34201 gacatcgtcc agcttgaagg gttttataat cgcatcaatc tttttcatgc tattccttga
    34261 aaaggttgcc tgtcttttga tctgctaaac gtaacacata acgccaattc attccttgaa
    34321 atcgtttgca tccagctcgt gtcgggaaag cagtttataa aattctgtcc ggttgcgccc
    34381 cgccattctc gccgcgtggg tgacgttgcc tttggtgatt tgcagcagct tacgcaaata
    34441 gttgagttca aaatgattac gtgcctcaac aaaggttggc agcgccgtat tttcaccctc
    34501 cagcgcctgc tccaccagcg catcactaat caccggagat gaggtcagcg ccacgcactg
    34561 ttcaatcacg ttgaccaact ggcgcacatt acccggccag ctcgcggtca tcaggcgttt
    34621 catcgcatcg gtagagaacg cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa
    34681 caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc gccagtgccg gaattttcag
    34741 gctgacaacg ttgaggcggt aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt
    34801 tgacagatca cggtgagtgg cagaaataat ccgcacattg atatcaatat cgcggttact
    34861 gcccagcggg cgcactttac gctcctgcaa cacgcgcagc agtttgacct gtaacggcgc
    34921 aggcatatcg ccaatttcat cgagaaatag cgttccgcct tccgccgcct ggaataaacc
    34981 ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca tgaccaaaca gctccgactc
    35041 cagcaattgc tcgggcaatg cgccacagtt aatagcaata aatggtttgc tgttgcgcgg
    35101 gctggcgttg tggatagcct gggcgaaaat ctctttcccg gtgccgctct gaccgttaat
    35161 caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag
    35221 cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg gtggctggcg cggattgctc
    35281 cagcgcatcg tcaattgcct gatatagcgc atctttatcg acaggcttgg tgaggaaact
    35341 aaaaacgccc tgctgtgttg cagcaacggc atcggggata gaaccatgcg cagtaagaat
    35401 aattactggc attcccggct gcactttctg gatttcagca aatagctgca taccgtccat
    35461 ttcatccatc cgcaggtcgc tgataactaa atctactttt tcgcgattca gtacccgtaa
    35521 tccttcagcg ccactttccg ccgtgaccac actgtagcct tcgctggtca ggcgcaggcc
    35581 aagcagtttc agcaatccca gatcgtcatc gaccaataat aaatgcgcag gtttatggct
    35641 catcaggagt gacctcatgg gtggatggcg cgggcttttc actctcgtgc ggcgtatccg
    35701 gcgagaaatt tccagccggt ttgcgggtcg agagctggcg ttcaatatcg gtcaggtttt
    35761 ccagcttgcg ggtggtgagt tccagttgct gctgtagaac gtgatgttgc tggcgcaatg
    35821 tatccagctc gctgtcgctg gactgctgga gtttactgta acgttggcgc tcttccgcca
    35881 gttgcagttg cagcgcctga ccgtcgcgcc agagttgata cagtgggcga acctgtgccg
    35941 ggatctcggt acttaacgct tcaatacgcg cgaccagctg gcggcgctca tacggcgtaa
    36001 ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat
    36061 attggcgagc ttgctgacgc gactgcgcag gcattaaacg atcagcacaa tccatcgccc
    36121 gcagccagta aagcggattg gtttcggttg atttaccttg cagcgcccag atgtcgctac
    36181 attcagtaga aagatagtca gccagttgat aaaccggaat tttttcttct gctggcgtat
    36241 caatggctgg cttattgtga ttctgcacgc aacccagcaa tgccagacat ggcagccctg
    36301 ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg tcgcatattc accagactta
    36361 aagcctatcc cagtgggcgt aattgttgca gacagtctgg acatggacag cgcggagaaa
    36421 ccggggcgta catatcgtac gtgaagattt cgagcactac ccggggccga actgacaaat
    36481 aaaatagcct gataggatag gctgttagca ttatttcgtg tttttcgacg acggtaattc
    36541 aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga tacagttccc cttgcatacg
    36601 gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg cccttcaccg ccccttttcg
    36661 ctggtggctt ccctgaaaaa aggggtcgaa gatcatggcg cgttcctctt gcggaatggg
    36721 cgtgcctgta ttgatgacat caatataaac ccgcgcacca tgtaaactgc tgcgaaggca
    36781 aatgttaccg gattcagccc cgtagtgcac cgcattggag taaagattat ccagtacgct
    36841 catcagcagc attggctccg ccaggcaagc tgttgctttg agatcgacgt cggtatgcat
    36901 cattttagcc cgtgcgggca ggctatgagc agaaaccact gtctccacca gcggtgctaa
    36961 ttcaacattc tccagttcca ccgcactgtc cgcctgttta cggttgtaat caagcagttg
    37021 ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga atgctcacca cctctttttg
    37081 ctctggcgta agcggcccga caacctgatc agccagtaat tcagtgccct cgcgcatact
    37141 cgccagtggc gtttttaatt catgagataa atgtcttaaa aattgatggc gttgggattc
    37201 cagccatgac aggcgctcac ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct
    37261 cggtccacag aacgaaacgc tattgcccag agaacgccct tcccccagac ggttgatcat
    37321 gcgctcgata tttttcaccg gcccgataat catccgcgtg aaaagtagca ccatcaccag
    37381 actcaccaga aatagcacca gcgattgcca accaaaatat tgcccacgtt cggcgatttc
    37441 acgctgaagt tgctgcccac gagagaacac cactgtgcgc gtggcctgca ccatttcggt
    37501 attggcactg gcaaaggctt caagacgcgc ggcggcggca gcatcgggac cactgttgtt
    37561 acactgaagt tgggccagat tgttcaaatc ctgacgcaat gcctggtaaa gtttatcgtc
    37621 tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa cgcttgcgct ggctttgata
    37681 aaccttcgca agcgtagggt cgtccagcac gcaatactga cggtagctac gctccatctc
    37741 cagcgccgcg ttggtcatcg cttcactacg ccgggcatca ataagcgtag tgcggttaac
    37801 cagcgccgcc tgatcgctaa gcgcattcag gctttgccag gcttgccatg ccagcaccaa
    37861 caggggtagt agaattagca aaaatgccag cattaccagt tgtcgtaatg agcggggaaa
    37921 aacgggccag cgtttcaagg tgttactctc gtcagacgcg aatagcctga tgctaaccga
    37981 ggggaagttc agatacaaca aagccgggaa ttacccggct ttgttatgga ataaggcggt
    38041 gcctaactcg acgtttcgcc cgatggttga tatagctacg ctgatatcag aagttggacg
    38101 gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca cgtcccgaag gggctgacat
    38161 aagtcggtga atgagccact gcttactatt atgcagtaac tgtgccaata aagaaaatag
    38221 tttggtaacg tactgattat acgttgcttt gagggattta tggcttcttc gctatttgaa
    38281 tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa gataaactta ttagattaca
    38341 ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg taaaccaccc attgtcgtga
    38401 tttacagaca caaaaaagct cccggagttg ggagcttatg atagtggttg gtgcttaatg
    38461 ccggatgcgg cgtaaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc
    38521 aactgcttac gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc
    38581 ggatgccagg agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg
    38641 actcgaccac tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc
    38701 gggtaggttt cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg
    38761 ctttccagtg ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag
    38821 acagcaatcg gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt
    38881 acttcaacca ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc
    38941 cacaactcac tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat
    39001 acccccaggg ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg
    39061 tcattgaaca ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag
    39121 aaacctccgc acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg
    39181 tcgctcatat gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca
    39241 acatgcgagt tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca
    39301 atatacggtg ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga
    39361 tcggcgtcgt tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg
    39421 cgctgcatct gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg
    39481 aatacagtct gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca
    39541 taatggacgc aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca
    39601 gcggcacgaa cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg
    39661 tcacccagag aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc
    39721 agcgttacca gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca
    39781 accagcgcct gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc
    39841 ggtttgtcgc caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg
    39901 cctttgccca aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg
    39961 gtgtgacgta catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc
    40021 atttcgcgct cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc
    40081 accgggatcg tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca
    40141 tacaggcccg catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta
    40201 atgcgtttga tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc
    40261 agacgggcag aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc
    40321 gcttcaccgt agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc
    40381 tgccacggcc ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg
    40441 gtcaccagga aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct
    40501 gcaatggtga tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg
    40561 cgggtcatct tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta
    40621 tcaaaatgac gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg
    40681 tagggtgcgc gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc
    40741 gccagcacgt agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt
    40801 tcgtcgctta gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc
    40861 tccggcatcg cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta
    40921 gcatcaccaa gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg
    40981 ttgtcgcgct gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa
    41041 gacgccgcgc cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc
    41101 gcaccgacgt tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc
    41161 gccagcatga tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt
    41221 tcttcataag tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc
    41281 gcaccgccca gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta
    41341 ccgaaatctt cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa
    41401 ccaaccaaac cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca
    41461 ccgccggaac cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt
    41521 tcgactttca tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg
    41581 tcagcaaagt agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca
    41641 gagagaacgt gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc
    41701 ggctgttgtt caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca
    41761 gagttcgcct gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg
    41821 aaagcatcct gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta
    41881 gcgtcgatca gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt
    41941 tgatggtggg caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg
    42001 cggtcgtgca gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct
    42061 atatagtaag caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca
    42121 atatcggtcg ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg
    42181 agtttgcctt gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt
    42241 tgtgcgtgct catcatcgtt taacgacgca ttgaggtcag caaaatggac atactcggcg
    42301 taaatattgt gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg
    42361 aatgccgaca gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag
    42421 ctttcggtgt acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    42481 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    42541 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    42601 ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat
    42661 tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg
    42721 ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag
    42781 cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg
    42841 ggaaaccttt tggcctagat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa
    42901 aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta
    42961 acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc
    43021 ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc
    43081 cacgtacgct gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtgg
    43141 ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg
    43201 acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca
    43261 ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc
    43321 tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc
    43381 tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca
    43441 ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt
    43501 tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag
    43561 aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg
    43621 ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc
    43681 tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc
    43741 aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc
    43801 tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa
    43861 ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga
    43921 aaccctatta cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg
    43981 tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc
    44041 aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt
    44101 tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga
    44161 cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg
    44221 tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac
    44281 tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca
    44341 tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg
    44401 caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg
    44461 gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac
    44521 cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt
    44581 tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc
    44641 gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccaatat tcgtggctaa
    44701 attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac
    44761 gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat
    44821 gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc
    44881 gcagcggcgt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca
    44941 tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg
    45001 gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca
    45061 accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa
    45121 aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg
    45181 cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac
    45241 gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat
    45301 tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca
    45361 atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct
    45421 ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat agatcatcac
    45481 ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag
    45541 cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt
    45601 tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac caagggcttg
    45661 cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt
    45721 taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg
    45781 cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg
    45841 caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt
    45901 tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa
    45961 acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga
    46021 taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga
    46081 tgaagattgg cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac
    46141 aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt
    46201 taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct
    46261 ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac
    46321 aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc
    46381 tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg
    46441 aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg
    46501 tgttgttttg agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat
    46561 atgtgtgaaa tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga
    46621 atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca
    46681 ccactttctt taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg
    46741 tgacatacaa cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc
    46801 ggttttatat aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga
    46861 tgatcgccac cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt
    46921 attctggtga aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca
    46981 gtcctgcttt tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca
    47041 catggaagaa aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa
    47101 agttgatgcc tcgttcgcgc aactcctcca ccagcacgac aagatgccgc atactgcgcc
    47161 ccagccgatc cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt
    47221 ttttcagccc cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc
    47281 atcctgcaca gttcaacgca ttacgttgta gatcggtatt ctggtcattt gttgatacgc
    47341 gtacatagcc aataagcatg gtaaatcccc ctgttaacaa gacactgcgt caaaagtcgg
//
LOCUS       DAECOD010000022        45299 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.022, whole genome shotgun sequence.
ACCESSION   DAECOD010000022 DAECOD010000000
VERSION     DAECOD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.022"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            96..395
                     /locus_tag="KKO15_001243"
     CDS             96..395
                     /locus_tag="KKO15_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6360102.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="KKO15_001244"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="KKO15_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBD6360103.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="KKO15_001245"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="KKO15_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBD6360104.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="KKO15_001246"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="KKO15_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBD6360105.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="KKO15_001247"
     CDS             complement(2534..2878)
                     /locus_tag="KKO15_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBD6360106.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="KKO15_001248"
     CDS             3030..3260
                     /locus_tag="KKO15_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360107.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="KKO15_001249"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="KKO15_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBD6360108.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="KKO15_001250"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="KKO15_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBD6360109.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="KKO15_001251"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="KKO15_001251"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBD6360110.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="KKO15_001252"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="KKO15_001252"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBD6360111.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="KKO15_001253"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="KKO15_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBD6360112.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="KKO15_001254"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="KKO15_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBD6360113.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="KKO15_001255"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="KKO15_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBD6360114.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="KKO15_001256"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="KKO15_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="KKO15_001257"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="KKO15_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBD6360115.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="KKO15_001258"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="KKO15_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBD6360116.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="KKO15_001259"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="KKO15_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBD6360117.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="KKO15_001260"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="KKO15_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="KKO15_001261"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="KKO15_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBD6360118.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="KKO15_001262"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="KKO15_001262"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBD6360119.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="KKO15_001263"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="KKO15_001263"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD6360120.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="KKO15_001264"
     CDS             complement(19399..19827)
                     /locus_tag="KKO15_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6360121.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="KKO15_001265"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="KKO15_001265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBD6360122.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20538..20614)
                     /locus_tag="KKO15_001266"
     tRNA            complement(20538..20614)
                     /locus_tag="KKO15_001266"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20578..20580),aa:Arg,seq:acg)
     gene            complement(20679..20755)
                     /locus_tag="KKO15_001267"
     tRNA            complement(20679..20755)
                     /locus_tag="KKO15_001267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20719..20721),aa:Arg,seq:acg)
     gene            complement(20759..20851)
                     /locus_tag="KKO15_001268"
     tRNA            complement(20759..20851)
                     /locus_tag="KKO15_001268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20815..20817),aa:Ser,seq:gct)
     gene            complement(21167..21352)
                     /gene="csrA"
                     /locus_tag="KKO15_001269"
     CDS             complement(21167..21352)
                     /gene="csrA"
                     /locus_tag="KKO15_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBD6360123.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21587..24217)
                     /gene="alaS"
                     /locus_tag="KKO15_001270"
     CDS             complement(21587..24217)
                     /gene="alaS"
                     /locus_tag="KKO15_001270"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBD6360124.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24345..24845)
                     /gene="recX"
                     /locus_tag="KKO15_001271"
     CDS             complement(24345..24845)
                     /gene="recX"
                     /locus_tag="KKO15_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBD6360125.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25088..26149)
                     /gene="recA"
                     /locus_tag="KKO15_001272"
     CDS             complement(25088..26149)
                     /gene="recA"
                     /locus_tag="KKO15_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBD6360126.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26229..26726)
                     /gene="pncC"
                     /locus_tag="KKO15_001273"
     CDS             complement(26229..26726)
                     /gene="pncC"
                     /locus_tag="KKO15_001273"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBD6360127.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26871..27956)
                     /gene="mltB"
                     /locus_tag="KKO15_001274"
     CDS             complement(26871..27956)
                     /gene="mltB"
                     /locus_tag="KKO15_001274"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBD6360128.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGEMETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28212..28774
                     /gene="srlA"
                     /locus_tag="KKO15_001275"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28212..28774
                     /gene="srlA"
                     /locus_tag="KKO15_001275"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            28771..29730
                     /gene="srlE"
                     /locus_tag="KKO15_001276"
                     /gene_synonym="gutE"
     CDS             28771..29730
                     /gene="srlE"
                     /locus_tag="KKO15_001276"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBD6360129.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            29741..30112
                     /gene="srlB"
                     /locus_tag="KKO15_001277"
                     /gene_synonym="gutB"
     CDS             29741..30112
                     /gene="srlB"
                     /locus_tag="KKO15_001277"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBD6360130.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30116..30895
                     /gene="srlD"
                     /locus_tag="KKO15_001278"
     CDS             30116..30895
                     /gene="srlD"
                     /locus_tag="KKO15_001278"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBD6360131.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31000..31359
                     /gene="gutM"
                     /locus_tag="KKO15_001279"
                     /pseudo
     CDS             31000..31359
                     /gene="gutM"
                     /locus_tag="KKO15_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            31426..32199
                     /gene="srlR"
                     /locus_tag="KKO15_001280"
                     /gene_synonym="gutR"
     CDS             31426..32199
                     /gene="srlR"
                     /locus_tag="KKO15_001280"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBD6360132.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32192..33157
                     /gene="gutQ"
                     /locus_tag="KKO15_001281"
     CDS             32192..33157
                     /gene="gutQ"
                     /locus_tag="KKO15_001281"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBD6360133.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33154..34668)
                     /gene="norR"
                     /locus_tag="KKO15_001282"
     CDS             complement(33154..34668)
                     /gene="norR"
                     /locus_tag="KKO15_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBD6360134.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            34855..36294
                     /gene="norV"
                     /locus_tag="KKO15_001283"
     CDS             34855..36294
                     /gene="norV"
                     /locus_tag="KKO15_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBD6360135.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36291..37424
                     /gene="norW"
                     /locus_tag="KKO15_001284"
     CDS             36291..37424
                     /gene="norW"
                     /locus_tag="KKO15_001284"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBD6360136.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37552..39804)
                     /gene="hypF"
                     /locus_tag="KKO15_001285"
     CDS             complement(37552..39804)
                     /gene="hypF"
                     /locus_tag="KKO15_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBD6360137.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(39957..40484)
                     /gene="hydN"
                     /locus_tag="KKO15_001286"
     CDS             complement(39957..40484)
                     /gene="hydN"
                     /locus_tag="KKO15_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBD6360138.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40633..41643)
                     /gene="ascG"
                     /locus_tag="KKO15_001287"
     CDS             complement(40633..41643)
                     /gene="ascG"
                     /locus_tag="KKO15_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBD6360139.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41657..41776)
                     /locus_tag="KKO15_001288"
     CDS             complement(41657..41776)
                     /locus_tag="KKO15_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBD6360140.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(41786..43495)
                     /gene="hycE"
                     /locus_tag="KKO15_001289"
     CDS             complement(41786..43495)
                     /gene="hycE"
                     /locus_tag="KKO15_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBD6360141.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43513..44436)
                     /gene="hycD"
                     /locus_tag="KKO15_001290"
     CDS             complement(43513..44436)
                     /gene="hycD"
                     /locus_tag="KKO15_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBD6360142.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44439..>45299)
                     /locus_tag="KKO15_001291"
     CDS             complement(44439..>45299)
                     /locus_tag="KKO15_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD6360143.1"
                     /translation="VGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMP
                     VISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITG
                     ALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPM
                     LSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYD
                     HEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAV
                     LVVIIVSRGA"
BASE COUNT        10797 a        12217 c        11474 g        10811 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taatcggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcacgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatatcgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga
     1981 tgacaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtggttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaaggaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gtcttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaatgac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg atacatcggg aaacttactt tactgcagat
    20641 tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga
    20701 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg
    20761 gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg
    20821 ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac
    20881 ttgtttatcg ctcatcatcg ctgcgtggcg cacatattac tttctgagac ttataagtca
    20941 aacaattttt cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaagc
    21001 gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa
    21061 aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag
    21121 aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact
    21181 gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct
    21241 tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga
    21301 cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct
    21361 ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc
    21421 caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta
    21481 aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta
    21541 atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg
    21601 cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac
    21661 cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac
    21721 caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag
    21781 aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta
    21841 aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca
    21901 gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag
    21961 cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc
    22021 gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc
    22081 gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac
    22141 gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc
    22201 ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta
    22261 cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga
    22321 gattcatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt
    22381 cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca
    22441 ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg
    22501 cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat
    22561 caatatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt
    22621 gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt
    22681 tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca
    22741 gcacgaccac agcttcctgg cctgcattga tggcatcaac cgctttacca tcaacaaaca
    22801 gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat
    22861 caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac
    22921 gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac
    22981 aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt
    23041 caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc
    23101 gctccagagt acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct
    23161 gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt
    23221 tgtagaagaa ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga
    23281 tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga
    23341 acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg
    23401 cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag
    23461 agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg
    23521 gcttcggcag cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt
    23581 tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa
    23641 tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca
    23701 tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac
    23761 gttcgcgcgg gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat
    23821 agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt
    23881 cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca
    23941 tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac
    24001 cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc
    24061 caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt
    24121 tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga
    24181 gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac
    24241 gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga
    24301 ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt
    24361 cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc
    24421 ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg
    24481 cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg
    24541 gaaatacctt tctgattcag ttcctgacga atacgtgcag gtccataccc tttgcggcta
    24601 cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac
    24661 caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg
    24721 cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc
    24781 agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga tgttgattct
    24841 gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcgc cccaggtttt
    24901 gctgataaac agatttaata gtcaatgagt atgcagaatg ttcccgcagg cccaattcgt
    24961 tagaggatcg aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa
    25021 ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa
    25081 cgaacgatta aaaatcttcg ttagtttctg ctactccttc gctgtcatct acagagaaat
    25141 ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg
    25201 cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct
    25261 cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt
    25321 caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat tcagcctgtt
    25381 taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt
    25441 tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt
    25501 tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac
    25561 ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac
    25621 gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt
    25681 cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc
    25741 cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtatcc ggctgggagc
    25801 acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt
    25861 cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg
    25921 tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca
    25981 gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat
    26041 ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct
    26101 ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg
    26161 tcatgccggg taataccgga tagtcaatat gttctgttga aacaattata ctgtatgctc
    26221 atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta
    26281 gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca
    26341 ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca
    26401 tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgca
    26461 cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct
    26521 tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca
    26581 aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa
    26641 gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt
    26701 aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac
    26761 aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag
    26821 aaaaaagctg attagccaga gggaagctca cgcccccctc ttgtaaatag ctactgtact
    26881 cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg
    26941 ttgtaacggg tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca
    27001 acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa
    27061 cctgcggcgg caagctgcga aatgctgtac ttagttttga aaccatttgg caagcctgga
    27121 gcctgaccgt ttgccattat cgcgacctga tcgcctttca cccagccgtg cgctttgaaa
    27181 tagttcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg
    27241 ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc
    27301 ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg
    27361 aaggtttcca tctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt
    27421 gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg
    27481 ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca
    27541 tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt
    27601 ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt
    27661 gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga gagaatttcc
    27721 tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca
    27781 ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg
    27841 aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg cttgctgcta
    27901 caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg
    27961 ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacacc ccgcacaagg
    28021 aagcggtagt cactgcccga tacggacttt acataactca actcattccc ctcgctatac
    28081 ttttattcaa actttcaaat taaaatattt atctttcatt ttacgatcaa aataacactt
    28141 ttaaaccttt caacctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga
    28201 aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc
    28261 aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc
    28321 tgcaggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg
    28381 ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt
    28441 ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa aagtacaaac
    28501 ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata
    28561 tcaaccctgc gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct
    28621 gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca atttcttccg
    28681 cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga
    28741 acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg
    28801 ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc
    28861 accgccggta cccggcctgc gattgttgac aaactggcac aacttactgg ctggcaggct
    28921 attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac
    28981 tgtggcggca cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc
    29041 cactcgacgg gcaagtccgg tccgctggcg cagtacattg tggaagatat ttatgtctct
    29101 ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg
    29161 ggccgtgact atgacaccag taagaaaatc accgaacaaa gcgatggttt actggcgaag
    29221 gtgggaatgg gcatggggtc cgccgttgcc gtgttgtttc agtctggtcg tgacaccatc
    29281 gacactgtat taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc
    29341 attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct agcgagccat
    29401 ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cactgctttc acctttcctc
    29461 ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc
    29521 ggcaatattc cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggct
    29581 tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc
    29641 ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg cgccgactgt actgatcgcc
    29701 tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac
    29761 caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac
    29821 ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga
    29881 gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt
    29941 gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg
    30001 cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc
    30061 cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa
    30121 tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct
    30181 ggctgccgag gggtatcgcg tcgcggttgt cgacattcag agcgacaaag ccgcaaatgt
    30241 ggcacaagaa attaacgccg aatatggtga aggtatggcg tacggttttg gtgctgacgc
    30301 cactagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt
    30361 ggatttgctg gtctatagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct
    30421 cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga
    30481 attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa
    30541 atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt
    30601 cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact
    30661 gatgctcggt aacctgctga aatcgcggat gttccagtca ctgttgccac aatacgcgac
    30721 caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa
    30781 acgcggctgc gattatcaag atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc
    30841 gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt tctgatcaac
    30901 agcggagatc cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc
    30961 aggcatacag gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcacc
    31021 gtcgccgtta tcgcctagtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt
    31081 aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtgggccg ttccagcggg
    31141 cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg atcagcagtg catcgtcgac
    31201 accttgttta tgaaaggact gactgtcttc gcccgaccgc aaaaaattcc cgcaattacc
    31261 ggcatgcatt cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag
    31321 aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg
    31381 cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag
    31441 cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg
    31501 gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat
    31561 gccggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg
    31621 cctatcgatc ataaaacgct catcaacacc cacaagaaag agctgattgc agaagctgcc
    31681 gttagtttta tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag
    31741 atggttcccc tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc
    31801 gtcaatgcgc tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt
    31861 cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc
    31921 ttcgataaat tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc
    31981 aacgaggttt ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg
    32041 gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc
    32101 gataagctaa ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa
    32161 gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga acacgggacg
    32221 tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga
    32281 ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat
    32341 tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc
    32401 ggcttttttt gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg
    32461 cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc
    32521 gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct
    32581 gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca
    32641 ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc
    32701 ggtcatgcag gcgcgcggtt ttaatgaaga agattttgcc cgctcccacc cagctggggc
    32761 actgggcgct cgcttgctga ataaagtgca tcatctgatg cgtcgtgatg acgctatccc
    32821 acaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct
    32881 ggggctggtg gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga
    32941 tttacgtcgc tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac
    33001 gacaggcggc accacattac aggcacaaag tcgcgccatc gacgccaaag aagttctgat
    33061 gaaacgcaaa atcactgccg caccagtggt agatgaaaac ggcaaactca ccggcgcaat
    33121 taacctgcag gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc
    33181 cagccgatgc aggttggcga cgtcagtttc cagcattcgc gcacaggctg cccagttgtg
    33241 atgattttgt gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg
    33301 caggttttgc ttaacaacga gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg
    33361 aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag
    33421 cactaccgcc cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg
    33481 ttgcagtaaa tttcgcgccc cggcacttaa taccacgcgg gagagcccct gccgcaaacg
    33541 acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg
    33601 cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc
    33661 cagcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa
    33721 actacggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc
    33781 ctgcaatgcc aacgacaact cgccgatctc atcgagaaac agagtaccgt tatccgccat
    33841 ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa
    33901 caactcactt tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt
    33961 caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt
    34021 ctcaccgctg atcagaacgt tgagatcgga cgccgccaca atctcaatct cttttttcag
    34081 ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt
    34141 ggcatcgcct ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa
    34201 cgctcccgcc gccagcgcgg caatcagccg taactcttcg tcgctgaaaa catcgaactg
    34261 atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgtccga caaacaatgg
    34321 cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg
    34381 atcgggcaat tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc
    34441 cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc
    34501 gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc
    34561 cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg
    34621 ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact
    34681 gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact
    34741 cattaatgag cataatttta tttatagagt aaaaacaatc agataagaaa ctggcacgca
    34801 atctgcaatt agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct
    34861 attgtggtga aaaataacat tcattgggtg ggtcaacgtg actgggaagt gcgtgatttt
    34921 cacggcacgg aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa
    34981 gaaaaaaacg tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac
    35041 ctgcgtaatg aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag
    35101 gaccacgccg gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt
    35161 actgccaacg ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat
    35221 gtggtgaaaa ctggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa
    35281 acaccaatgc tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg
    35341 ttcagtaacg atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg
    35401 gatcagacgg agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc
    35461 agccgcctgg taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg
    35521 atagcgacct cccacggcgt ggtctggcgc gataacccga cgcaaattgt cgagctgtac
    35581 ctgaagtggg ccgctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg
    35641 aataacaccc gcatgatggc tgacgctatc gcccagggaa ttgcggaaac cgacccacgc
    35701 gtggcggtga aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc
    35761 ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa
    35821 atcgccgggc tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct
    35881 ttcggatctc acggctggag cggcggtgcg atggatcgcc tttccacgcg cctgcaggat
    35941 gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc gaccagacca ggacgctctg
    36001 gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg
    36061 cagagcacgg tgaatacggt agttaaagaa gaaacctctg ccacgacgac ggctgacctc
    36121 ggcccacgaa tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca
    36181 atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctctgcccg
    36241 gaatgctccc tcggcaaaga cgtctttgac gaactggcat cggaggcaaa atgagcaacg
    36301 gcattgtgat catcggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac
    36361 aggacgccac tattccatta accctgattg ccgccgacag catagatgag tacaacaaac
    36421 ccgacctcag ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg
    36481 cgggtgaatt tgccgggcag tttaatctgc gcctgtttcc gcacacctgg gtgacggata
    36541 tcgatgccga agcccatgtg gtgaaaagcc agaataatca gtggcaatac gacaagttag
    36601 tgctggcaac cggtgccagc gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga
    36661 cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg
    36721 tgttgattgt cggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag
    36781 gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccag
    36841 aagtaagcag ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat
    36901 ctcagttgca ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc
    36961 gtagtatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg
    37021 cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca
    37081 gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt
    37141 tcctgcagcc gattcaactt agcgggatgg tgctggcaaa aaatcttctc ggcaataaca
    37201 cgccgctgaa actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc
    37261 tggcaggcga aacccagcgt caggatttac gctggcagat taataccgaa cgccagggaa
    37321 tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc
    37381 ggatgaaaga ggcatttgta ttgttgaaaa cgttgccggt gtaggtgggc tactgtgcct
    37441 aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc
    37501 ggataaggca tttacgccgc atccggcaag ggtgtccaaa tgcaacacgc tttatccgtt
    37561 ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc
    37621 gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc
    37681 cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat
    37741 accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg
    37801 aaacgcccac gcgcgttgat taaccagtgc ctgccagttc agccactgct gccagaaagt
    37861 ggcgagatcc agttgattgt ccacccgcgg catcgtcacc ggatgcgtca ctccgtcgca
    37921 tgaggctgcg agcgcctcca gagcacaagc cgcttcacct tcataactta acgtggctgg
    37981 cgcacagccc agtgccgccg ccactgcatc gaacaaccgc ccacacgatg atgccagcgg
    38041 cgcgttaatt ccacgctcaa tggcccgcgc cagtacgctc cagttttgct gttgcacact
    38101 tgccgtttca gggtaattct gccactccgg cacaaagcgc aggcactgcg ccagcaggtt
    38161 tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc gccactgcag gcaagccgcc
    38221 caggtgctca cattcgcgat agttcacccg caggcactcg ccgccccaca aagcgccatt
    38281 ctcccccata ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat ccagcggcca
    38341 ctgatgctct gccagacacg ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg
    38401 cagattcatt tcgctcgccc actggctgga gacatagccc ggatgcacat catgcacaat
    38461 gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc gccactgcat
    38521 ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt gttcaccgcg
    38581 caccaggcag aaggtatttt tcagatccgc gccaagacac agcacaggcg gaacattttt
    38641 aaagcccgga ggcaaaacca gcgcatccgg cacatacccc cgcgaacggc gcagcatttc
    38701 gccgctttcg cgcaccactg aatcatccat ccgctgcacg atgtcgcggt tatgtagcaa
    38761 gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg
    38821 tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct gtaacagcaa
    38881 atgctggagc gggttcgcag gcaacatcac cccgacttcg ttaaggccag gggcgatatc
    38941 atcacaaagc tcaggtacgt attttttatc caccagcaca atcggcgcag cgggcgtggt
    39001 aagcaactgg cgcgcagcgt ctggtaaact ttctgccact ggcaacatga ctgccagcgg
    39061 tttcgccggg cgatgtttgc gctcccgcag tgtcgccacc gcgttactgt tacgtgcatc
    39121 gcaggcaaga tgaaatccgc caatcccttt gatggcgaca atgttgccca tttttaactg
    39181 tgcgatagct gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac ttacccattc
    39241 aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac gatcgagcgg
    39301 gtcgcggtac tctttgtcac aggccggaca tagcggaaac gccgccatca cggtaaacgg
    39361 gcggtcgtaa ggcatcgcgc gaataatggt gaaacgcggg ccgcagtggg tacagttgat
    39421 aaacggataa cgataacgcc gctcgcctgg ggtattcatt tcggcaaggc aagcagggca
    39481 agtggcggca tccgggacaa tttgcgtatt catggtgccg ccggtgctct ggcggatggt
    39541 gaactcggtg ggcagttgtg accagataaa cggctcacgc tcgacgctat caatacgcgc
    39601 cagtggcgga cagtgctgat gcaattgaac aagaaacgtt tccgggtctt ccagcagccg
    39661 gacttctacg ccatcgccgt cattacatac atcgccgtga agatttaatt gctgtgccag
    39721 ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac gcagttggac
    39781 accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg cgtaacccgc
    39841 cctgccggac acgacagcgt cgtatccggc agtctcaggt cggcgatacc gcccgctccg
    39901 tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta agatttttag
    39961 aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt attacgatcg
    40021 acacaaatca gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg
    40081 ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc gtacattcag acccgcgccg
    40141 ctgttgcgaa tcacaggacg taccaccact tccatcgcgc catacgggca agccaccacg
    40201 caggttttgc aaccaatgca acgttcctgc atcacaggaa caaacccttt atcacggctg
    40261 atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg gcaaactgtc
    40321 gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc
    40381 agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc acaggtacgg
    40441 caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat
    40501 gacagttaat gcgccgatac attcacaaat catgccagtt tttaatttgc tgttatttaa
    40561 ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga cgatgacatg
    40621 tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg cggcggatca gttttccgct
    40681 aaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat
    40741 ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa cgatataggg
    40801 ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg
    40861 ctcgtgcaac gctttgatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct
    40921 aaatttagcc cctcgttcga gcaacgtttc taccccttcg gccccgctgg cgggcgtcca
    40981 tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt
    41041 atacccagca agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat
    41101 ctcctgatgc ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc
    41161 gcaccagacg ctatggctgc tattattgcg caggcggcga ttaagcacca ttatcggctg
    41221 actgtgcgcg tcaatgatgt catcaatctc atccacgctt aaaaaacgcg ggtaaatcat
    41281 aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct
    41341 gtgtttacca tctgccaata gcaactgtcg ccctttctct tccgccattc gcgcggcatg
    41401 aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta ccagccccag
    41461 cgtctgagta ctcttcgccg acagattgcg ggccagcaaa tttgggcggt aactgctctc
    41521 ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag
    41581 cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt
    41641 catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc
    41701 cttgcgcaat cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt
    41761 tgataaaggt aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt
    41821 aacgctcgag ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg
    41881 tcatgcggtc ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatcgg
    41941 aaacggtatt gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac
    42001 ggcagcgcca gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc
    42061 cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca
    42121 ccatcagcgg accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat
    42181 agacctcgtt gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga
    42241 cttccattgg cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga
    42301 gaccgctggc gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt tccgggtcca
    42361 gacgaccaat gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca
    42421 gctcctgcac ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt
    42481 tcagcagatc gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg
    42541 taaggatctc tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg
    42601 agtcgaagcc ggtaaagtga caggcgaggc cgaggttgag cagatgcggg tgcaggcgtt
    42661 ccacctccag cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg
    42721 cgttttccac cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac
    42781 ggtcagagag gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac
    42841 cgcgatggac gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca
    42901 gacggaagtg acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt
    42961 ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg
    43021 gacgctgacg ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat
    43081 ccggcagcac cagacgacgt tcatccggca gaccaaccgg aatcaaaccg tacatatcgc
    43141 gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg cgtcacggac ggatactccg
    43201 gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc ttctccatcg
    43261 acagcacgta ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca
    43321 ccgacagcca gccaccctgc ttgtagtaga gaaactccac cacttccggc agatagttca
    43381 cctttacggt gacagtcagc tgatctttgg tctgccaggc gtggtccagc acgacgccta
    43441 gaaatgcctc attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca
    43501 aactctcttt aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc
    43561 agcccaggta atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag
    43621 cgcgataacc aggacgccga ccaccagttt gacgatggca atcaccagcg ccagcagcag
    43681 tccaccgacg gtgaaggttt ccatttgtcc ccacggaata aacaccccga cgaacatctg
    43741 caacaccacc agctgtttca ggctgatacc ccatttcatg acgccaaagc cgctaccgct
    43801 gtattcggag agcggacctt cctgcaactc ctgctcggct tccgccaggt cgaacggcag
    43861 tttgcccatt tcgataaaag tggcgaacgc acaggcgcag agcgccagca ccagcgggat
    43921 gctttggctc agcggccagt gataaacggt gtcggtaatg ttgctgatgt tggtggaacc
    43981 ggcaacctgt gcggcaaccc acagaccaag cagcaacatc ggttcgacca gcacgccaag
    44041 catcgcttca cggctcgcac cgatagcggt aaacgggcta ccggtatcca gaccggaaat
    44101 ggcaaagaag aaacgcgcga tggcgaagag atacagtaag gtgatcaaat cgcccagttg
    44161 cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc
    44221 caccatcaca tacggcgtca ggcggaacat ccagccagag gcatccgggc caacgctctg
    44281 gcggcccagc agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt
    44341 atgcagacgg gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaataa
    44401 caccagcgcc tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata
    44461 atcaccacca gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactacct
    44521 tcgcactgcc agcccggcac tagcgacacc ggattcagcc atttgcgcag tttcagcact
    44581 ggcgcaaacg cctgtttaac cggcatggca aaaccgtgag cggtaatcac cattgatttt
    44641 tcatggtcgt agccgcacac ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct
    44701 ttgcaaatcg ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc
    44761 attggttgag aaacggtggt gttagcaggc tccagaggca gaggtacagc agcagagagc
    44821 atcggcagca gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact
    44881 acgcttacgc tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc
    44941 gcgccgagga acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta
    45001 attgccagac ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg
    45061 ctcagtttga agaaggattg atagataacc cattccccgg caaacccgtt cagtggcggc
    45121 agcgcagcca ttgccatcaa cccgactaac atggcgatgg agataaccgg cattttcttg
    45181 ccaataccac cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc
    45241 ccgaggaaca gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgac
//
LOCUS       DAECOD010000023        43131 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.023, whole genome shotgun sequence.
ACCESSION   DAECOD010000023 DAECOD010000000
VERSION     DAECOD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.023"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            114..2267
                     /locus_tag="KKO15_001292"
     CDS             114..2267
                     /locus_tag="KKO15_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD6360144.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="KKO15_001293"
     CDS             2320..4050
                     /locus_tag="KKO15_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360145.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="KKO15_001294"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="KKO15_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBD6360146.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="KKO15_001295"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="KKO15_001295"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBD6360147.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="KKO15_001296"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="KKO15_001296"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBD6360148.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="KKO15_001297"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="KKO15_001297"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBD6360149.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="KKO15_001298"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="KKO15_001298"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBD6360150.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="KKO15_001299"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="KKO15_001299"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBD6360151.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="KKO15_001300"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="KKO15_001300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBD6360152.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="KKO15_001301"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="KKO15_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBD6360153.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="KKO15_001302"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="KKO15_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBD6360154.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="KKO15_001303"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="KKO15_001303"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBD6360155.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="KKO15_001304"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="KKO15_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBD6360156.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="KKO15_001305"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="KKO15_001305"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBD6360157.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="KKO15_001306"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="KKO15_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBD6360158.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="KKO15_001307"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="KKO15_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBD6360159.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="KKO15_001308"
     CDS             16308..16847
                     /locus_tag="KKO15_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD6360160.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="KKO15_001309"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="KKO15_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBD6360161.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="KKO15_001310"
     CDS             complement(18206..18553)
                     /locus_tag="KKO15_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBD6360162.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="KKO15_001311"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="KKO15_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBD6360163.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEEPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="KKO15_001312"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="KKO15_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBD6360164.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="KKO15_001313"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="KKO15_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBD6360165.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="KKO15_001314"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="KKO15_001314"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBD6360166.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="KKO15_001315"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="KKO15_001315"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBD6360167.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="KKO15_001316"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="KKO15_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="KKO15_001317"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="KKO15_001317"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBD6360168.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="KKO15_001318"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="KKO15_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBD6360169.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="KKO15_001319"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="KKO15_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBD6360170.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="KKO15_001320"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="KKO15_001320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBD6360171.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="KKO15_001321"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="KKO15_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBD6360172.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="KKO15_001322"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="KKO15_001322"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBD6360173.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSCEQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="KKO15_001323"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="KKO15_001323"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBD6360174.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEKLTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTVLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="KKO15_001324"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="KKO15_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBD6360175.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="KKO15_001325"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="KKO15_001325"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBD6360176.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="KKO15_001326"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="KKO15_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBD6360177.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="KKO15_001327"
     CDS             complement(40011..40466)
                     /locus_tag="KKO15_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360178.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="KKO15_001328"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="KKO15_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="KKO15_001329"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="KKO15_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBD6360179.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="KKO15_001330"
     CDS             complement(41309..41986)
                     /locus_tag="KKO15_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBD6360180.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="KKO15_001331"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="KKO15_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBD6360181.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="KKO15_001332"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="KKO15_001332"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBD6360182.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT        11245 a        11196 c        10538 g        10152 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gttcttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtt gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgagaag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgcccta gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gtgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct c
//
LOCUS       DAECOD010000024        39562 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.024, whole genome shotgun sequence.
ACCESSION   DAECOD010000024 DAECOD010000000
VERSION     DAECOD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.024"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(63..3509)
                     /gene="mfd"
                     /locus_tag="KKO15_001333"
     CDS             complement(63..3509)
                     /gene="mfd"
                     /locus_tag="KKO15_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBD6360183.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3637..4710)
                     /locus_tag="KKO15_001334"
     CDS             complement(3637..4710)
                     /locus_tag="KKO15_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBD6360184.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFIYTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            4972..6171
                     /gene="lolC"
                     /locus_tag="KKO15_001335"
     CDS             4972..6171
                     /gene="lolC"
                     /locus_tag="KKO15_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBD6360185.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6164..6865
                     /gene="lolD"
                     /locus_tag="KKO15_001336"
     CDS             6164..6865
                     /gene="lolD"
                     /locus_tag="KKO15_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBD6360186.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6865..8109
                     /gene="lolE"
                     /locus_tag="KKO15_001337"
     CDS             6865..8109
                     /gene="lolE"
                     /locus_tag="KKO15_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBD6360187.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8138..9049
                     /gene="nagK"
                     /locus_tag="KKO15_001338"
     CDS             8138..9049
                     /gene="nagK"
                     /locus_tag="KKO15_001338"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBD6360188.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9065..9886
                     /gene="cobB"
                     /locus_tag="KKO15_001339"
     CDS             9065..9886
                     /gene="cobB"
                     /locus_tag="KKO15_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBD6360189.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(10042..11088)
                     /gene="potD"
                     /locus_tag="KKO15_001340"
     CDS             complement(10042..11088)
                     /gene="potD"
                     /locus_tag="KKO15_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBD6360190.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(11085..11879)
                     /gene="potC"
                     /locus_tag="KKO15_001341"
     CDS             complement(11085..11879)
                     /gene="potC"
                     /locus_tag="KKO15_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBD6360191.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(11876..12733)
                     /gene="potB"
                     /locus_tag="KKO15_001342"
     CDS             complement(11876..12733)
                     /gene="potB"
                     /locus_tag="KKO15_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBD6360192.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(12717..13853)
                     /gene="potA"
                     /locus_tag="KKO15_001343"
     CDS             complement(12717..13853)
                     /gene="potA"
                     /locus_tag="KKO15_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBD6360193.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14103..15329
                     /gene="pepT"
                     /locus_tag="KKO15_001344"
     CDS             14103..15329
                     /gene="pepT"
                     /locus_tag="KKO15_001344"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBD6360194.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(15378..16499)
                     /gene="roxA"
                     /locus_tag="KKO15_001345"
                     /gene_synonym="ycfD"
     CDS             complement(15378..16499)
                     /gene="roxA"
                     /locus_tag="KKO15_001345"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBD6360195.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            16748..17977
                     /locus_tag="KKO15_001346"
     CDS             16748..17977
                     /locus_tag="KKO15_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6360196.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLNLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            18352..18540
                     /locus_tag="KKO15_001347"
     CDS             18352..18540
                     /locus_tag="KKO15_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD6360197.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(18590..18919)
                     /locus_tag="KKO15_001348"
     CDS             complement(18590..18919)
                     /locus_tag="KKO15_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360198.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(19044..19223)
                     /locus_tag="KKO15_001349"
     CDS             complement(19044..19223)
                     /locus_tag="KKO15_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360199.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            19353..19553
                     /locus_tag="KKO15_001350"
     CDS             19353..19553
                     /locus_tag="KKO15_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBD6360200.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19543..19764
                     /locus_tag="KKO15_001351"
     CDS             19543..19764
                     /locus_tag="KKO15_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360201.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            19757..19977
                     /locus_tag="KKO15_001352"
                     /pseudo
     CDS             19757..19977
                     /locus_tag="KKO15_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19979..20212
                     /locus_tag="KKO15_001353"
     CDS             19979..20212
                     /locus_tag="KKO15_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360202.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            20218..20517
                     /locus_tag="KKO15_001354"
     CDS             20218..20517
                     /locus_tag="KKO15_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD6360203.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            20514..22262
                     /locus_tag="KKO15_001355"
     CDS             20514..22262
                     /locus_tag="KKO15_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD6360204.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIAILLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            22551..22829
                     /locus_tag="KKO15_001356"
     CDS             22551..22829
                     /locus_tag="KKO15_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360205.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            22826..23131
                     /locus_tag="KKO15_001357"
     CDS             22826..23131
                     /locus_tag="KKO15_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360206.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            23141..23302
                     /locus_tag="KKO15_001358"
     CDS             23141..23302
                     /locus_tag="KKO15_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBD6360207.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            23444..23668
                     /locus_tag="KKO15_001359"
     CDS             23444..23668
                     /locus_tag="KKO15_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360208.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            23969..25126
                     /locus_tag="KKO15_001360"
                     /pseudo
     CDS             23969..25126
                     /locus_tag="KKO15_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            25166..25738
                     /locus_tag="KKO15_001361"
     CDS             25166..25738
                     /locus_tag="KKO15_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBD6360209.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDPSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            25740..26951
                     /locus_tag="KKO15_001362"
     CDS             25740..26951
                     /locus_tag="KKO15_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBD6360210.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVDQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            26948..27286
                     /locus_tag="KKO15_001363"
     CDS             26948..27286
                     /locus_tag="KKO15_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD6360211.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            27283..27579
                     /locus_tag="KKO15_001364"
     CDS             27283..27579
                     /locus_tag="KKO15_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBD6360212.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            27579..28019
                     /locus_tag="KKO15_001365"
     CDS             27579..28019
                     /locus_tag="KKO15_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6360213.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            28003..28185
                     /locus_tag="KKO15_001366"
     CDS             28003..28185
                     /locus_tag="KKO15_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360214.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            28241..28567
                     /locus_tag="KKO15_001367"
     CDS             28241..28567
                     /locus_tag="KKO15_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBD6360215.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            28551..30212
                     /locus_tag="KKO15_001368"
     CDS             28551..30212
                     /locus_tag="KKO15_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD6360216.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            30226..30507
                     /locus_tag="KKO15_001369"
     CDS             30226..30507
                     /locus_tag="KKO15_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360217.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(31304..32764)
                     /gene="phoQ"
                     /locus_tag="KKO15_001370"
     CDS             complement(31304..32764)
                     /gene="phoQ"
                     /locus_tag="KKO15_001370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBD6360218.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(32764..33435)
                     /gene="phoP"
                     /locus_tag="KKO15_001371"
     CDS             complement(32764..33435)
                     /gene="phoP"
                     /locus_tag="KKO15_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBD6360219.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(33604..34974)
                     /gene="purB"
                     /locus_tag="KKO15_001372"
     CDS             complement(33604..34974)
                     /gene="purB"
                     /locus_tag="KKO15_001372"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBD6360220.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(34978..35619)
                     /gene="hflD"
                     /locus_tag="KKO15_001373"
     CDS             complement(34978..35619)
                     /gene="hflD"
                     /locus_tag="KKO15_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBD6360221.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(35655..36761)
                     /gene="mnmA"
                     /locus_tag="KKO15_001374"
     CDS             complement(35655..36761)
                     /gene="mnmA"
                     /locus_tag="KKO15_001374"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBD6360222.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(36815..37276)
                     /gene="nudJ"
                     /locus_tag="KKO15_001375"
     CDS             complement(36815..37276)
                     /gene="nudJ"
                     /locus_tag="KKO15_001375"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBD6360223.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(37286..37939)
                     /gene="rluE"
                     /locus_tag="KKO15_001376"
     CDS             complement(37286..37939)
                     /gene="rluE"
                     /locus_tag="KKO15_001376"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBD6360224.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            38111..39361
                     /gene="icd"
                     /locus_tag="KKO15_001377"
     CDS             38111..39361
                     /gene="icd"
                     /locus_tag="KKO15_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBD6360225.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(39468..>39562)
                     /locus_tag="KKO15_001378"
     CDS             complement(39468..>39562)
                     /locus_tag="KKO15_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6360226.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        10038 a         9784 c        10452 g         9288 t
ORIGIN      
        1 aggaaagtgc aaagaatttg taaatgttgc agatgggggc gcagaaacgc ccccgattta
       61 cattaagcga tcgcgttctc ttccagttca cgcataaact ggcgtaccca ttcgatacgc
      121 gttttccgct cactcaaatc ctgaataaat ttcaggcgcg tcggaccatc aaggcggtaa
      181 tgctgcggct gtttttgcag caaaccaatc aaccaggccg gattaacgtg attcttctcg
      241 gcaaattcga tcaccccgcc tttctcatta ccttccagct tcctgatccc cagtttctgc
      301 gcttgctggc gcagacgggc aatatccagc agggtacgcg ccggatctgg cagcaggccg
      361 aagcgatcga taagctcgac tttgatctct tccagttcgt tttccgtttt ggcgctggca
      421 atacgtttgt agaacgacag acgcgtgttc acgtcaggga tgaaatcatc tggcaatagc
      481 gacggcatcc gcagctcgac ttctgtttgc tggctggtga gatcttccag cgacggctcg
      541 cgtccggctt tcagtgcatc gacggcgttt tccagcaact ccatatacag cgagaaaccg
      601 atggtttcca ttgagccgct ttgttcttcg ccaagcagtt cacccgcgcc gcgaatctcc
      661 agatcgtgcg ttgccagcgc aaaacctgcc ccgagatctt ccagcgaggc aatcgcttca
      721 agacgttttt gtgcatcggt agtcatcgct tttggatgcg gcgtcagcag ccatgcatat
      781 gcctgatgat gtgaacgtcc gacgcgaccg cgtaactggt gcagctgcgc cagaccgaag
      841 tgatccgcac gttcaatgat aatggtgttg gctgtcggga tgtcgatccc ggtttcgata
      901 atggtggtac aaaccagcac gttgaaacgt tgatgatgga aatcattcat cacccgttcc
      961 agttcgcgct cgcgcatctg cccgtgaccg atggcaatcc gtgcttcagg caccagttct
     1021 gccagccgtt cggcggcttt ctgaatgttt tccacatcat tgtagagata ataaacctgt
     1081 cctccgcgca aaatttcacg caggatcgcc tcccggacca ccaggctgtc atactcacgg
     1141 acaaaggttt taactgccag acgacgggcg ggcggcgtgg cgataatcga caggtcacgc
     1201 attccgctca ttgccatatt cagcgtacgt gggatcggcg ttgcagtaag cgtcagaata
     1261 tccacgttcg cgcgcatcgc tttaatgcgc tctttatgac gcaccccgaa gcggtgttct
     1321 tcatcgacaa tcagcaggcc taaatcttta aacttgacgt cactttgcag cagtttgtgc
     1381 gtaccgatca gaatatcgat tttcccttcc gccacttccg caaggatttg cgtctgctct
     1441 ttggcgctgc ggaaacggga gatcatttcg atacgtaccg gccagttggc gaaacggtcg
     1501 cggaagttgt cgtaatgctg ctgcgcaaga agggtggtag gcaccaacac cgctacctgc
     1561 ttgtggttat ctactgccag gaaagcggcg cgcatcgcca cttctgtttt accaaagcca
     1621 acatcgccgc acaccagacg atccattgcc agcggctgac acatgtcgct aagtaccgca
     1681 ttaatggcct gcgcctgatc cggcgtggtt tcaaacggga agctgtcgca gaataactga
     1741 tactgctcac gatcgtgttt aaacgcgaag ccctctttgg cggcgcgttg cgcgtagata
     1801 tccagcaatt ccgccgccac atcacgcact ttttccgccg ctttctgccg cgcgcgtgac
     1861 cacgcatcgc cgccaagttt atgcagcggg gcgttttctt ccgcgccacc tgcgtaacgg
     1921 ctaatcagat gcagtgacga caccggaaca tacagtttgg cgtcgttggc ataggtgagc
     1981 atcaggtact cgccagtaat gccgccagct tccagcgtgg tcattccggc atagcgaccg
     2041 acgccgtgct ccagatggac caccggctga ccaatatgca gctccgcaag gttacggatc
     2101 agtgtatcgg ggttgatggt gcggcgagaa tcctgacgac ggcgggcaac acgttcaccg
     2161 agcagatcgc tttcgcagat cagcgccaga ttacgcgcct tatcgacaaa accatgttcg
     2221 gcagcgccaa tcatcagata acgcccacgg tcgctggctt catcaagacg cataatgcgt
     2281 tgcggagcaa ttttaattcg tgcgagcagt tcacccagcg cttcacggcg accttcactt
     2341 tctaccgaga acaccaccgg accgtcgaaa gtctcgagga acttacgcag cgcatccagc
     2401 ggcgcttttt gttgcgcctg aacggccagg tctggcagtt tctggaaacc taaattggca
     2461 ttcgcggctt ttgtcggtaa atgttcagtt tttagctgca ctcggggcca gtttttcagc
     2521 tctgagaaga gctcgtccac ccgcagccag agcgattgtg gtggcaacag cgggcgcatc
     2581 ggatcgacgc cgcgattctc aaaacgcgcc agcgtgtcag cctggaaacg ttcggcactg
     2641 gtttccagat cgccagtatt cacgagcaag gtattggcag ggaaataact gaacagcggc
     2701 ggcagtggtt cgctgaagaa caatggctgc cagtactcga tcccagcagg taatgtgcct
     2761 ttactcactt gctggtaaat atgttccgga tcgcgcttca cttcgaaggt atcgcgccac
     2821 tggctgcgga acagttcaat tgccgcttta tcggtcggaa attcgtgcgc gggcagcaga
     2881 ttgatcgctt ctacttcctc cagcgtgcgc tggctgtcga cgtcaaacac ccgcaggctg
     2941 tcgatttcat catcaaagaa atcaagacga taaggcagct cactccccat cgggaagaga
     3001 tccagcaacg cgccgcgcgt ggcgtattcg ccgtgctcca tcacctggtc aacatggcga
     3061 taaccggcgc tgtccagttg ggttcgtaat gcatctcgtg acaggcgctg accttttttc
     3121 atcaccagcg catgaccgtg gagaaaactg tgtgggcaaa cgcgctgcat aagcgtattc
     3181 accggaacaa tcagtatgcc acgctgcatc gtcggtagct ggtaaagggt ggaaaggcgc
     3241 gaggagataa tgtcctgatg aggcgaaaaa ctgtcgtagg gaagagtttc ccagtccgcc
     3301 agattcatca ccatctgatc ggtgaactgg ctgatttcat catgcaaacg cagagcattt
     3361 tgcatatctg gtgcaatgag taccaccgga ccggcgtgac gttcggcaat ttccgctacc
     3421 agcgttgcac aggctgcgcc ggttaactcg cccagcagac gctgctcacc cgctttgacg
     3481 ggcagcgtat aacgatattg ttcaggcata acggttgtca gattctcgtt aggatatgcc
     3541 tcaacatatg ggggcatatc tctgataagc aatgctgttc attatccgta atcgacatgg
     3601 tttagcaaat tgaatcgccc cggctggggt gatggtttaa cgagagtaac tgacggcagc
     3661 gggcagtgaa ggcggggaga acaacagatc gctgaatgca cgctgtgaaa gttttcttac
     3721 cagcaagccc gcaacagtat agatgaacaa actgacaaag ggataaacca gcaggagtgt
     3781 cagttcgact tgcggcgacc agcgtgctgc gttcatttgc gcaagcagag ttaagctgaa
     3841 tatttcaacc agaatgcgat gggtggtgta gatagcaatg gtgttggagc caatcacatt
     3901 cagcaggctg gtggagcgca taccgaatcg ttgctcgtac tgataaaaca gcttcatgat
     3961 caccacaatc gataccagcg acaacagcag cgagatagta aacaaccagg caccgaccgc
     4021 cagaacggta agcaaagaag ccatcagcag atggcggcgc aacggcacct ctttaacaca
     4081 ggtcattatt gttgcgccaa accatgcgcc aaggctgtaa taaggcaaat tgcgtatcac
     4141 actgttcatt ccccaccacg gcgtgggaac gaaattaacc gccacactca gcagtacaaa
     4201 caaggcgaat agtggcagcg ccagacggct aaaaatttta cataccacga aatagacaat
     4261 taacgcatac agataccaca agctggtgct ggcggtgatc atcccgtgca ggaactcacc
     4321 ggtggaatcg gcataagcgg cattggaggc attgcttaaa tcgcgctcag gtgccagcca
     4381 ctggttcagc gcacttaacg ccagccactg caccacgccc caaagcgcca gcacccagaa
     4441 gatgttccag atccgtttat cgagacaatt tccccacggt acgctgtcga tatagcggcg
     4501 aatcaaatag ccggatataa agaaaaaaac cggcatacga aagggggcaa ggtaaagatt
     4561 gaaatagatc cagcatttgc tcaggacttc cgataacgga tgctgaaaag tggtcatatg
     4621 cggataaaag gtaatgaccg agtgataaat caccaccaga caaatacata accctttgat
     4681 ctggttaatc catagctctt tttgtttcat cagaacaaca cgctcttttt taatttacat
     4741 gcggttgatc ctgctggtta tgacccgagg tgtaatgggt atctgtctgt attggatgat
     4801 ttttcagatt aagatcaggc ggcaagattg atgataaaac atggcaattt agccgattga
     4861 tttacggggg cttttcagat tagccctgac gatcacttac agttcagacg tttacccatc
     4921 ttgctttcgc ttatatactc gtgtctttgc tacagcaacc agacggattt catgtaccaa
     4981 cctgtcgctc tatttattgg cctgcgttac atgcgtgggc gtgcagtgga tcgcttcggt
     5041 cgtttcgtct cctggctttc taccatcggc attaccctcg gggtgatggc gctggtcaca
     5101 gtattgtcag tgatgaacgg ctttgagcgc gagctgcaaa acaacatcct tggcctgatg
     5161 ccacaggcaa ttctctcttc tgagcatggc tctcttaacc cgcagcaact cccggaaacg
     5221 gcagtcaaac tggacggcgt taatcgcgtc gcacctatta ctaccggtga tgtggtactg
     5281 caaagcgcgc gcagcgtggc ggtcggggtg atgctcggta tcgacccggc gcaaaaagat
     5341 ccactaacgc cgtatctggt caatgtgaaa caaactgacc tcgagccggg gaaatataat
     5401 gtcatcctcg gcgaacaact tgcctcacag ttgggcgtca atcgcggtga tcaaatccgc
     5461 gtgatggtgc catctgccag ccagttcacg ccgatggggc gtattccaag tcagcgcctg
     5521 ttcaatgtga ttggcacttt cgccgccaac agtgaagtcg atggctatga aatgctggtg
     5581 aatattgagg atgcctcgcg tctgatgcgt tatccggcag ccaatattac cggctggcgt
     5641 ttgtggctgg atgagccgct gagagttgac tctttaagtc agcaaaaact gcctgaaggc
     5701 agcaaatggc aggactggcg tgaccgtaaa ggcgagctgt tccaggccgt acgcatggaa
     5761 aaaaatatga tgggcttact gctgagcctg attgtcgctg ttgcggcgtt taacattatt
     5821 acctcgctgg ggctgatggt gatggagaag cagggcgaag tcgcgatcct gcaaacgcaa
     5881 ggcttaactc cgcgacaaat catgatggtc tttatggtgc aaggggctag cgccgggatt
     5941 atcggtgcaa tcctcggagc ggcgcttggc gcactgcttg ccagccagtt aaataatctg
     6001 atgccgataa tcggcgtcct gcttgatggc gcggcgctgc cggtggctat cgaaccttta
     6061 caggtcattg ttattgcgct ggtggcgatg gctatcgcgc tgctgtctac gctttaccct
     6121 tcatggcgcg ctgccgccac acaacccgct gaggctttac gttatgaata agatcctgtt
     6181 gcaatgcgac aacctgtgca aacgctatca ggaaggcagt gtgcaaaccg atgtgctgct
     6241 caatgtcagt ttcagcgtgg gcgaaggtga aatgatggca attgtcggta gctctggttc
     6301 cggtaaaagt accttgctgc acctgttagg cgggctggat acgccaacct ccggcgatgt
     6361 gatctttaac ggtcagccga tgagcaaact gtcttcagcg gcgaaagcag aactgcgcaa
     6421 ccagaagctg ggctttattt atcagtttca ccacctgctg ccggatttta ctgccctgga
     6481 aaacgtggct atgccactgc tgattggcaa gaaaaagccc gctgaaatca acagctgtgc
     6541 actggagatg ttaaaagcgg tggggctgga gcatcgtgcg aatcaccgcc catctgaact
     6601 ttctggcggc gaacgccagc gtgtagcgat tgcccgtgcg ctggtcaata atccgcgtct
     6661 ggtactggcg gatgaaccta ccggtaacct cgatgcgcgt aacgccgaca gcatcttcca
     6721 gttgcttggg gagttgaatc gcttgcaggg caccgctttc ctggtggtca ctcacgacct
     6781 gcaactggcg aaacgtatga gccgccaact ggaaatgcgt gatggacgtc tgacggcgga
     6841 actgagcctg atgggggcgg agtaatggcg atgcctttat cgttattgat tggcctgcgt
     6901 tttagtcgcg gacggcgacg cggcggcatg gtgtcgctaa tctctgtcat ttctaccatt
     6961 ggcattgccc tcggcgtggc ggtattgatc gtcggcttaa gcgcgatgaa cggctttgaa
     7021 cgcgaactga ataaccgcat tctggcggtg gtgccgcatg gcgaaatcga agcggtaaat
     7081 cagccatgga ctaactggca ggaagcactg gataacgtgc agaaagtgcc gggtattgcc
     7141 gccgctgcgc cgtatatcaa tttcaccggt ctggtggaaa gtggagcgaa tctgcgcgca
     7201 atccaggtga agggcgttaa cccacaacag gaacagcgtc tgagcgcatt accctcgttt
     7261 gttcaggggg atgcgtggcg caattttaaa gcgggcgaac agcaaattat catcggcaaa
     7321 ggcgtggcgg atgcgctgaa agtgaagcag ggcgattggg tgtcgatcat gatccccaac
     7381 tcgaatcccg agcataaact gatgcagcca aaacgtgtgc gtttgcacat tgccggtatt
     7441 ttgcagttga gtggtcaact cgatcacagt tttgccatga tcccgctggc ggatgcccaa
     7501 caatatcttg atatgggttc cagcgtgtca ggtattgccc ttaaaatgac ggatgttttc
     7561 aacgccaata agctggtacg cgatgcgggt gaagtgacca acagctatgt ttatattaaa
     7621 agttggattg gtacttacgg ctatatgtat cgcgatatcc agatgatccg cgccattatg
     7681 tatctggcga tggtactggt gattggcgtg gcctgtttca acatcgtctc caccttagtg
     7741 atggcggtga aagacaagag tggcgatatc gcagtattaa gaacgctggg ggcgaaagat
     7801 ggtttaattc gcgccatctt tgtctggtat ggattgctgg cagggctgtt cggcagcctg
     7861 tgtggggtgg ttatcggcgt agttgtttca ctgcaactta ccccgattat tgagtggatt
     7921 gaaaagctga tcggtcatca gttcctctcc agcgatattt attttattga cttcctgcca
     7981 tcggaattgc actggctgga cgtcttctac gtactggtca cagcattgtt gctgagtctt
     8041 ttggcaagtt ggtatccggc gcggcgcgcc agtaatattg accctgcgcg agtccttagc
     8101 ggccagtaaa ggcagtacat taaaacaagg agcggcaatg tattacgggt ttgatattgg
     8161 tggaacaaaa attgcgcttg gcgtgtttga tagcggtcgg cagttgcagt gggaaaagcg
     8221 ggtgccgaca ccgcgtgaca gctatgacgc atttttagat gcagtgtgtg agctggtagc
     8281 tgaagctgac caacgttttg gctgtaaagg ttctgtcggc atcggtattc cgggaatgcc
     8341 ggaaacagaa gatggtaagc tgtatgccgc taatgtcccc gctgccagcg gcaaaccgct
     8401 gcgtgccgac ctgagcgcac gtcttgatcg cgatgtacgc cttgataacg atgccaactg
     8461 ttttgccctt tcagaagcct gggatgatga atttacgcaa tatccactgg tgatggggtt
     8521 aattctcggc accggcgttg gcggcgggct ggttttcaac ggcaaaccga ttactggtaa
     8581 aagctacatt accggcgagt ttggccatat gcgtctgccg gttgatgcgt taaccatgat
     8641 ggggttggat ttcccgttac gccgctgcgg ctgtggtcag catggctgca ttgaaaatta
     8701 tctgtctggt cgcggttttg cgtggctgta tcaacactat tatcatcaac cgttgcaggc
     8761 tcccgaaatt attgcgcttt atgatcaagg cgatgagcag gcaagggcgc acgttgagcg
     8821 ttatctggat ttattagcgg tttctctggg aaatatcctg accattgttg accctgacct
     8881 ggtcgtcatt ggtggtggct tatcgaattt cccggcaatc acaacgcaac tggcggacag
     8941 gttgccttgt catctcttac ctgtagctcg tgttccgcgc attgaacgcg cgcgccacgg
     9001 tgatgcggga ggaatgcgtg gtgcggcctt cctacatcta accgattaaa caacagaggt
     9061 tgctatgctg tcgcgtcggg gtcatcggtt aagtcgtttt cgtaaaaata aacgtcgcct
     9121 gcgcgagcgt ttgcgtcagc gtattttttt cagagataaa gtggtgccgg aagcaatgga
     9181 aaaaccaaga gtactcgtac tgacaggggc aggaatttct gcggaatcag gtattcgtac
     9241 ctttcgcgcc gcagatggcc tgtgggaaga acatcgggtt gaagatgtgg caacgccgga
     9301 aggtttcgat cgcgatcctg aactggtgca agcgttttat aacgcccgtc gtcgacagct
     9361 gcagcagcca gaaattcagc ctaacgccgc gcatcttgcg ctggctaaac tgcaagacgc
     9421 tctcggcgat cgctttttgc tggtaacgca gaatatagac aacctgcatg aacgcgcagg
     9481 taataccaat gtcattcata tgcatgggga attgctgaaa gttcgttgtt cgcaaagtgg
     9541 tcaggttctc gactggaccg gagacgttac gccagaagat aaatgccatt gttgccagtt
     9601 cccggcaccc ttgcgcccgc acgtcgtttg gtttggcgaa atgccactcg gcatggatga
     9661 aatttatatg gcgttgtcga tggcggatgt ctttattgcc attggtactt ccgggcatgt
     9721 ctatccggcg gctgggtttg tacacgaagc caaactacat ggtgctcaca ccgttgaact
     9781 gaatcttgaa ccgagtcagg ttggtaatga atttgccgag aaatattacg gcccggcaag
     9841 ccaggtggtg ccagaatttg ttgaaaagtt gctggaggga ttataaagca ggaagcattg
     9901 cctgatgcgc tacgcttatc aggcctacaa gcggaccaca ataccgacga aagagcggga
     9961 taaaacttta ctgtcctgac tcattagcca catccttgct aactaaaaaa cgggcggtaa
    10021 taccaccgcc tgcttgctga attaacgtcc tgctttcagc ttctgataat actcttcata
    10081 aatgctgctg gctgcgccaa cgtcattctg ccattcgcca tttttaatgg tttcagcatc
    10141 cgggtagagc gtcttatcgt ttgccacttc tggacttaac agcttacgcg ccgcaaggtt
    10201 tggcgttgga taaccgatag tttcagcaac ctgttttgcc acatccgggc gcagcaggaa
    10261 gttgatcaat ttcagcgcgc cttctttgtt tttggcattt gccgggatcg ccaggctgtc
    10321 catccagaat atgccgcctt ctttcggcca caccacgtca ataggcgtac ccgcctggcg
    10381 tgcaacgaaa gcagaaccgt tccagatcat gccgaggtta acttcgcctt ccatgtacgg
    10441 gttagccggg ttatcggagt taaacgctgc gacgtttggc atcagttttt tcagctcgtt
    10501 atatgcagct tcaatctctt tcggatcggt ggtgttaccg gagtagccca gcttacgcag
    10561 cgccatctgg aacacttcac gggcatcgtc ggtcaacagc aggctgcctt tgtactctgg
    10621 cttccacaga tcggcccagc tggtgacaga tttcggatcc accgcatcac cgttaacacc
    10681 aatcgccgtc gcaccccaga tatacggaat ggagtagtcg ttattcgggt caaaaggctt
    10741 gttgagcatg tctggatcga gattgctgaa gttcgttaac ttcgacttgt cgatcttctg
    10801 gatcatcccc tctttacgca ttttatcgac gtaataggtt gaaggaacca ccaggtcata
    10861 ggcaccgtct ttgtacgttt tcagcttggc gtacatggtt tcgttcgact cgtaagtcga
    10921 atagataacc ttaataccgg tttctttggt gaactgttca agcagtcctg gcggcacgta
    10981 ctcggtccag ttgtagaaat acagcgtgtt gttgtcatcg gcgtgagcgg cgctcatgcc
    11041 cagtgccaga gcacccgccg cgagcaggtg gcgtgaccat tttttcattt aacgtcccct
    11101 gtgttacctt tcgttttatc acgagcaata agctggctgg caataaccat caccagcgac
    11161 agcaccagta atatggttgc cagcgcgtta acttccggcg atacgccgac tttgaccatc
    11221 gaataaattt ttaacggcag aatttcataa cttggtccgg tgacgaatga agaaactacc
    11281 acatcgtcca tcgacagggt aaagcttaac acccagcccg ccgccaccgc tggcattgcc
    11341 agtggcagaa tgatttttcg cagaatggta aattcgctgg caccgagatc tttcgccgct
    11401 tccagcatcc gcacgtcaaa acctttcagg cgcgaataca ccgtcaccac cacaaatggc
    11461 aggcagaagg tgatatgcga gaacagcagc gaccagaagc caagctgaat acccagcagc
    11521 ataaacagca ccagcagcga aattgccatc acgatatctg gtgacatcat taccacaaac
    11581 agcattccgc taacgaacgg cttaccgcga aagcgataac ggtacagcgc aactgccgtc
    11641 agtgaaccga taagcgtagc aaacgtcgcc gaaaacaccg ccattgttag tgaatgctgc
    11701 gctgcctgta acaggctgtc gttgttcatc agcaggctat accatttggt ggtaaaaccc
    11761 tgccagttga tgccaaagcg cgagctgtta aaggagttca caatcaaaat aatgattggg
    11821 atatacaggt acgcgtagat agcggtcata aaaccgccgc gaagcagtcg accgatcatt
    11881 cgagttccac cttcttattc agcaaacgag aagcgcgcca gtaaaccagc aacatcaggc
    11941 ccattacgat agtcagcgta atgctggtgg ctgcaccaaa cggccagtca cgaatattga
    12001 ggaactgtac cttgatgacg ttaccgatca gcaggttttt cgcaccgccc atcaggtcgg
    12061 acacatagaa caggcccatc gctggcagca tcaccagcag acatccggca ataatccccg
    12121 gcatcgtcag tggaataatg atgcggataa aagtctgtaa cttgctggca ccgagatcgc
    12181 gcgccgcctc aagcaacggc ttatccagtt tttcgatact ggagtacagc ggcatcacca
    12241 taaacggcag cagaatgtaa accagaccga taatcaccgc actgggcgtg aacatgatac
    12301 ggattggtgt gtcgataacg cccagccaga gcaaaaactc gttgagatag cctttggtgc
    12361 tgaggaaaat tttcagcccg tagatacgaa tcaatgagtt ggtccagaac ggaacaatca
    12421 gcagaaacag cagcagcgga cgcaccttgt gtggcagctt cgccagaaac caggcaaatg
    12481 gatagcccag caccaggcag gcgagggtgg cgatcagcgc catattcagc gagtgcaata
    12541 gcacttcaaa atagagcgga tcgagcagac gcgtgtagtt atccagcgta aagaccattt
    12601 tgacgaaact ggcgtcgtcg cgggtcaaaa agctggtgcc aatgatcatc aggttgggca
    12661 gaaagacaaa caacacaagc caaccgacaa tagtgacaat cactacattc tggaacttac
    12721 ttgtgttctt catcagccag tacgacctcc cagctttcta cccaattaat ggccattttt
    12781 tggtcgagag agtggtcaaa gtcaggatcg tcttcattga agaattcgct gaccatcacc
    12841 atcttgccat tttccagttc aacaaccgac tccagcgtca tgcctttgta gttacgctca
    12901 cgaacgtaac caatcagccc ttcagcgtgg ttgtcgtcgt taatctcttc aacacgtaag
    12961 tcttccgggc gcagcagaac atgcagtttt tgccccggtt caacggcgaa gttaacgtag
    13021 atattacatt cgcggccttc aacgttggcg cgtacgcgct gctcgtcgag tcgctcgatc
    13081 accgtggcgt taaacatatt aatttcgcca atgaacccgg caacaaacag gtttttcggc
    13141 tcttcgtaga tttcacgcgg cgtgccgtct tgctcaatgc gaccatcgcg catcaccaca
    13201 atcctgtccg acatggtgag tgcttcttcc tggtcgtgag tcacgaaaac gaatgtaatg
    13261 ccaagcttac gctgtaacgc tttcagctcg ttctgcattt gcttacgcag tttgtaatcc
    13321 agcgctgaga gcgactcatc cagcaacaac agacgaggct tgttaaccac cgcgcgagca
    13381 atggcgacgc gttgctgttg accaccagag agctgatgcg gtttacgttg agcgaaggtt
    13441 tccagctgca ccatccgcag ggcttccatc acgcggggcg taatttcagc agcgggggtt
    13501 ttttgcatgc gcaacccaaa ggccacattt tcgaacacgg tcatgtgggg gaaaagtgca
    13561 tagctttgga aaacagtgtt cacatagcgg ttttccgccg gaacgtgggt gatgtcctcg
    13621 ttatccagca tgatgcgtcc ggaatcaaca gtttccagac ctgcaatcag gcgaagaacg
    13681 gttgttttac cgcagccaga agggccaagc agcgtgagga actcgccatt gttgatagtc
    13741 agatccagct ggggaatgac ctctttacca tcaaagcatt tgcgaattcc cgccaattgc
    13801 accagcggtg aaagcgaact cggttgttta ttcaattttt tactctgtcc catgtaaacg
    13861 caacggatgg cttaccgatg cggggtttgt ggttaaccac cttggtgact cttaatgagg
    13921 gcggtaattc tacggcaaac cgcttgaatc gccaatcttt gttgtgaatt actggcttag
    13981 ctttatattc attaaggtaa tgctgataaa tattcccgct tgcaggggta aaagtgacct
    14041 gacgcaatat ttgtcttttc ttgcttctta ataatgttgt cacaaaaagt gagggtgact
    14101 acatggataa actacttgag cgatttttga actacgtgtc tctggatacc caatcaaaag
    14161 caggggtgag acaggttccc agcacggaag gccaatggaa gttattgcat ctgctgaaag
    14221 agcagctcga agaaatgggg cttatcaatg tgaccttaag tgagaagggc actttgatgg
    14281 cgacgttacc ggctaacgtc cctggcgata tcccggcgat tggctttatt tctcatgtgg
    14341 atacctcacc ggattgcagc ggcaaaaatg ttaatccgca aattgttgaa aactatcgcg
    14401 gtggcgatat tgcgctgggt atcggcgatg aagttttatc accggttatg ttcccggtgc
    14461 tgcatcagct actgggtcag acgttgatta ccaccgatgg taaaaccttg ttaggtgctg
    14521 atgacaaagc aggtattgca gaaatcatga ccgcgctggc ggtattgcaa cagaaaaaca
    14581 ttccgcatgg tgatattcgc gtcgccttta ccccggatga agaagtgggc aaaggggcga
    14641 aacattttga tgttgatgcc ttcgatgccc gctgggctta cactgttgac ggtggtggcg
    14701 taggcgaact ggagtttgaa aacttcaacg ccgcatcggt caatataaaa attgtcggta
    14761 acaatgttca tccgggcacg gcgaaaggag tgatggtaaa tgcgctgtcg ctggcggcac
    14821 gtattcatgc ggaagttccg gcggatgaaa gcccggaaat gacagaaggc tatgaaggtt
    14881 tctatcacct ggcgagcatg aaaggcaccg ttgaacgggc cgatatgcac tacatcatcc
    14941 gtgatttcga ccgtaaacag tttgaagcgc gtaaacgtaa aatgatggag atcgccaaaa
    15001 aagtgggcaa agggttacat cctgattgct acattgaatt ggtgattgaa gacagttact
    15061 acaatatgcg cgagaaagtg gttgagcatc cgcatattct cgatatcgcc cagcaggcga
    15121 tgcgtgactg cgatattgaa ccggaactga aaccgatccg cggcggtacc gacggcgcgc
    15181 agttgtcgtt tatgggatta ccttgcccga acctgttcac tggcggttac aactatcatg
    15241 gtaagcatga gtttgtgatt ctggaaggta tggaaaaagc ggtgcaggtg atcgtccgta
    15301 ttgccgagtt aacggcgcaa cggaagtaag cgaaaaggga tgcggcatgt gatgctgcat
    15361 ccggcttaaa tccaaactta cccttcgaag aaccaatacc cgctattgac cagcgccgcg
    15421 agcatcgcga ggaatgacgg atcttccagc gcatcgccaa aattctccgc agtcagcgca
    15481 atgttgctgg cgagtgcatc cagtgccgga cggtgcgggg aatcgatctt ctcaccattg
    15541 gcatacacgt cgtcgccaat gcgcaatacg cgcagaccac ccaggcgcac cagcacatca
    15601 ccttgtttca gcgcatcgta gatttcatct ggctgataag gcggttccgg cggcgcgata
    15661 tccagttcat gacgtgactg ggatataaac tcgccaaacc attgcttaaa gtgttccggc
    15721 tggttgatca attcgagcat catctcacgc agtttatcca tctcttgcgg cagaacatcc
    15781 gcaggatgag cacgaggtgg aacatccgga tcgctgtagt agttgccgcc cagttcacgt
    15841 tgcagcacat aatcggcaaa tccgctaatt aattcccgcg tatttggcgc gcgaaaaccc
    15901 acggaatagt tcatcgcatt ttccagcgcg tagccttcat gcgggaatcc tggcggaata
    15961 taaagaatat caccaggctc cagctcttca tcgatgatgg cttcgaacgg atcgacctgt
    16021 aacagatccg gatgtgggca gtgctgtttc atttgcagct tttcgcccac tcgccagcga
    16081 cgacgtccgg taccctgaat gataaacacg tcgtactgat cgagatgcgg gccgacgccg
    16141 ccgccaggta cagaaaaaga aatcatcaga tcatcaatac gccagtccgg tagttcacgg
    16201 aacggtcgca tcagcgcggc ggtcggctca tgccagtggt ttactgcctg taccagtaat
    16261 gaccagttgg tttcaccgag atgatcgtag ctttcgaacg ggccgtggct gacctgccat
    16321 ttgccatcct ggtgactgac cagtcgactg tcaacttcgc tttccatcgc cagacccgcc
    16381 aactcgtctg gagagatcgg gtcaataaaa ttattaaagc cgcgttttaa caccaccggg
    16441 cgtttctgcc agtgacgttc aagaaaatcg ggccagttaa gagtgagttg gtattccatg
    16501 ttaagcatcc acaggctggt atctgcaacc gattataacg gatgcttaac gtaatgcgtg
    16561 aagtatggac atattttatc aattagtgtt ttttgccgtt tcataatgtt tcagattgtt
    16621 cattatgttt ttgattataa acagatttgt gattttcctt gttccagctt gtttcacgtt
    16681 gtagcccttt gtgcaacgat cggtgtagtt aatggtgtag ttaattgtga tgaccggaat
    16741 caaaattatg agcagagcac ttaacaaact gagcgataca cagctgagga aaatcaacgg
    16801 cacacccgcc caaaaaacag cctttcttaa tgacggtgga aacctgagcg tcaggcattc
    16861 aaccagtggc cttttaacct ggtatttcac ttacagggcc ggaacgggaa ggggggcacc
    16921 accggaacgc attaagctgg gaaattatcc tgatctgaac ctgaaatcag ccagggaaaa
    16981 agccgcccag tgtcgcgcat ggctggcaga ggggaaaaat ccacgtcatg agcttaatta
    17041 caccgtacag gaagcgttaa agccggtaac ggttggcgat gcgctcacct actggcttga
    17101 gtcgtacgca aaggaaaacc gcgtggatta tgccgccctg aaaaagcgcc ttaataatca
    17161 cgtaatacag cacattggtg ctatgccgct ggataaatgc gagctacggc actggctggc
    17221 ctgttttgac caggtggcaa agcgaacgcc tgttactgcc ggattcttgc tacagacgtg
    17281 caaacaggcg cttaagttct gccggaggcg gcgctatgca atcagcaacg ttcttgatga
    17341 tatgagtgtg gcggacgttg ggaaaaaacc ggatataagc gagcgtgtct taagcaccaa
    17401 agaactgggc gaattattgc aggcactgga caaaaaaata ttctccccct actacatcgc
    17461 gttaatccgc ctcctgattg tgttcggatg ccggacggta gaactgaggt tatcggagat
    17521 cagcgagtgg gattttaccg aaatgctctg gaccgtgccg aaagaacaca gcaaaacgaa
    17581 ggtggcaata ttccggccca taccggaagc aatactgccg ttcgtcacgc agctggtgga
    17641 gcagaacagg cacacgggct tattgctggg ggaagtgaaa caggaaacaa gcgtgtcgca
    17701 gtacggcaga ttagcgcaca ggaggctaaa tcaccctcac tggtcactac atgacatccg
    17761 gcgcaccttt acaacgatgc tgaacgattt aggcgtggat cctcacgtcg tggagcagct
    17821 tacaggccac cagatgccag gaatgcagcg agtttataat cattcccgtt atctggatgc
    17881 taaacgcaat gcgctggata tgtggacgga gcggttaggg atactggcgg gaacacatga
    17941 aaacgtaacc acgctaccag tagccagaag aaaataattt ttttcgtgtt ttttcagtat
    18001 gcgcatactg gatatgcgaa cagatacaac gtgcaacaat gagcaacaaa gcggaacaac
    18061 tacgaacaag aggtgaaaaa gtgtgcagat ttataagtag ctgattttta atgtgttact
    18121 gattttttat acactagcga atcactcttt aaatagcgag aaaaaaggca tgaaacaata
    18181 ttttatgcct ttgtattaac atgaatttaa aggattttat cctgattatt cctctttctg
    18241 taaaccatac ggcctcaaca tttgccaact ctgaatcatc ctgacacaat cagacacatc
    18301 ccaacgcaat agaacacgaa ataactcttt aagaaacgaa agggggcaat agtgttaagc
    18361 actgatcggt ttatacgtga aaaagaatgc gaaaagctaa caggccttag ccgctcatgc
    18421 cgctaccgcc tggaaaaggc cggacaattc ccatcacgtc gtaaacttgg cggtcgttcc
    18481 gttggctggt ctttatccga ggttctggcc tggaaggata gctgcaaggc agttcattaa
    18541 tcacgctggc ggcacgcagc cgccacacat caatcatctg aacacagagc tataaccatg
    18601 aagattgaat atacgccaga acgtgggcgg ggattcgttc gccctggtga aactggaaaa
    18661 ccacaaaatt ggggtttttc aggcataaaa aaagcggccc cgaaatggag ccgcctttct
    18721 gaacagataa cccgctgcgc cgtttgtgtg tgtgatccca aacataagca cggggatgat
    18781 agccgctatc aggctggtgg gcaatgcaat cagtctggtt cagtgcgttg ccataactgc
    18841 gaagttctct cctttgatga gtattcttta aggtacttct caagggcaaa agaacacggc
    18901 gcgaatcgtt ctgattcatg ctctttcttt ctgcgtcgtc tttttcgtgc aggtgatgtt
    18961 ttttcggttg attcttcgtt ggtcattgtg tgcgcctgta aagcaatgcg ccgtagtacc
    19021 tcacaccacg gcgctgatag tttttatcct ttgggttcta tgccgcgttg ctgtaattct
    19081 ttgcggatta ttcgctttat ccaggcagca agagaatcat cgccgtcctg ttgttgtgcc
    19141 ttttccatct tttcccgtaa gtcaggatct aaacggaact ggaacggagg attaccacgc
    19201 ctttcgtttt tgtgtgttga cacgttaatt acacctcagg taatgtattt atgtgtagtg
    19261 acacattaca cacaatcaac acaaaaagca aagccccgca ctgtaggagc agtaacgggg
    19321 cttctaacca acaacgtaaa ctaggagccg ttatggttgc cgtaaatcat ataccacacc
    19381 ttgtacacac acaaacggcc tttgtgtggc gttttctggc actgagtgcc ggagaatccc
    19441 aaatcatcca cgtaaccgct tggacggaac gcgaagcgcg tagccgttgc ccgtccggtt
    19501 gtgttgctgt attcgccgcc cgtattcgtc agggggaaac ctatgcacaa taaaaccaca
    19561 ccgaacgcag cagccgccgc actcactacg ctgatgcacg cgcttattga tattgaatgc
    19621 acggcagagc ttgcgcaggg agaagaacag aaagaccgga cacagttcgc cctggaatgt
    19681 atccgataca tcgcaacgcg gtcactgaat gacgctaaaa atattcttgt tgccgattgt
    19741 gaaaatgggg ggggttatgc gtgatgatcg ttttaattcc ctgaaacagg aattttccgg
    19801 cgttcctgat gatgcggctg atgcgctttc gtcaatgcca gaacttatta gagcggcttt
    19861 tttcttactt tccacgagag aatataaatc aacgggcttg atgtactgaa tatcgccgcc
    19921 gattatgctg atttcgtgac agaggttatt ttaagaaaaa cgacggacgg ggattaatat
    19981 gcgtgatatt taccacgaaa caatagaccg cgcatttctt gcactttctc acagtgaaaa
    20041 catgctggaa atattgcgca tatggcttga aacacttggc gacaatgaac gcgacaaaca
    20101 aaaatcaaga attgccacgg cattaataac gcttcttgag cctgtaataa tggaactgca
    20161 agaaatagat ctattgcacg acagatataa agaacaacac accggagaat aaaaataatg
    20221 aaacttaaat attctggctt aactgccagt ggcaacactc accctaaatt tacgcgcggt
    20281 gatatttacc gcgaccagta cggcggcatg gtaacgatta aaggcgtggc agaacggcgc
    20341 atcacctacc gccgtgaagg ttacgaatat gattgcgtga tgcctgttta tcaattccgg
    20401 cgtgatttca cgctggtaca ggcagcgccc cgcagtaaac caaccagcag ggagaaagca
    20461 cgcgccaata ttcaggaaat aaaaaagatg cttaacgtat tcaggggtaa aaaatgaaac
    20521 tggcaccgaa cgtaaaaaaa cagccacgcg gaataaaaca caaagacaca gaggtaatta
    20581 tctttgcggg tagtgatgcc tggtcgcacg cgaaacaatg gcaggagcag gatggccccg
    20641 catccggcga taatgtgccg cctgtgtggc ttgggccaaa tcagcttgcc gaacttgatg
    20701 cactgaaaat tgttccggat ggaaaaaaac gcgtaaggct gtaccaggcc ggagaactgg
    20761 atttggtgga gaccaaaaag attggtcaga agctggcggc ggcagatatt caggacgcaa
    20821 atttttaccc cgaaggaatg cacgtccaga agtgtgaaaa ctggcggcgc tatctgaatg
    20881 ctgagcgtga aaatattgcc gcagggctta ccatgccgga gcagaaaaat acgcaactgg
    20941 cacaaatggc agacagtgag cgcgcgcagc tgcttgccga gcgctttgat ggcgtttgtg
    21001 tgcatcagga aagtgaaatc gttcacgtat ggcgcggcgg ggtatggtgt ccggtcagca
    21061 caatggaact tagccgcgaa atggtggcga tctattcaga gcacagggcc actttcagca
    21121 agcgcgtaat caataacgcc gtggaagcgt taaaagttat tgccgaacca atgggcgagc
    21181 cgtccggcga tttgctgccg ttcgccaatg gtgcgcttga cctgaaaacg ggggaatttt
    21241 ccccgcacac gccggagaac tggatcacca cgcacaacgg cattgagtac acgccaccag
    21301 caccagggga gaacatccgc gataacgcgc taaactttca taaatggctt gagcacgcag
    21361 ccggaaaaga ccagcgcaag atgatgcgta tatgtgccgc gctgtacatg attatggcga
    21421 accggtacga ctggcagatg tttattgagg ccaccggaga cggcgggagc ggtaaaagta
    21481 cattcacaca catagccatc cttctggcag ggaaacagaa cacggtaagc gctgaaatga
    21541 catcgcttga tgatgctggt ggacgtgcgc aggttgtcgg gagtcgtctt atcgtcctgg
    21601 ctgaccagcc gaaatataca ggcgaaggaa cgggcatcaa gaaaatcacg ggcggtgacc
    21661 ccgtggaaat taacccgaaa tacgaaaagc gtttcacggc tgtaatcagg gcggtggtgc
    21721 tggcgaccaa caataacccg atgatattca ccgaacgggc cggaggtgtg gcacgtcgtc
    21781 gcgtgatttt ccgtttcgac aatatcgtta gtgaggccga aaaagacagg gagctaccgg
    21841 aaaagattgc ggctgaaatc cctgtcatta tccgccgatt gctggcgaac tttaccgact
    21901 ctgagaaagc acgggcttta ctactggaac agcgtgacgg tgatgaagct ctggcaataa
    21961 agcaacagac ggatccggtt attgagtttt gccagttcct gaattttctg gaggaagcac
    22021 gcggcctgat gatgggcggc ggtggcgatt cagtgaagta cacgaccaga aacagccttt
    22081 accgcgtcta tctggcgttt atggcgtacg caggcaggag caaaccgcta aacgtaaatg
    22141 actttggcaa ggctatgaag ccagccgcga aagtttacgg acatgaatat attacgcgga
    22201 aagttaaggg agtaacgcag actaacgcaa taacaacaga cgagtgcgac gcgtttttat
    22261 aattttttgt aattgctgtc taccctgtct acctgagtaa agaaaaatac atttaattca
    22321 gtgcattaag ttaggtagat agcctttttt tactgtctac ccactatcta ccctctctac
    22381 ctgattttac ctgaatcaga cagggaggta gacacggggt agatagtgga taaaagcact
    22441 ctaccccgct gaaagcagcg ccattactgg catgagggcc actaaggtag ataaggtaga
    22501 caaggggagg cacaactcaa aactttttaa acgagggggt aaaaataaat atgcacactt
    22561 cagggaaatt taataaatca ttcaaaaaac gcagagacag aacagaaccg aaatatcgcg
    22621 cgttagacat gacagagcac gctttaaagg tggcaatcag aacgatagac cgccacgcgg
    22681 gggaaggata cgcgaaagca catcccgaac tgataagcgc attcatgacc acgacggcgg
    22741 caaattttgc cacgctgaca gagcgggaga ttgccgaagc ggaacaggta acaacaatca
    22801 acgttaaaac cggagaggtg gaatcatgag cgaaccaaaa tttggtgaaa aattttacaa
    22861 gcacaacgga cggatcacga ttctgcaaat aagcgcagct acaccaggct ggtgggttga
    22921 aacagacgaa ggatcctcac ccgtggcatc atgggcgctt tgtgtgctta gttaccctga
    22981 ccgcgatgtg tatcaggata ttctgcctgt gatatccact gacaaaggca tgaaacccgt
    23041 agacatcaaa aacatggggt ttcagtgcgt aatgctcact gaaaaaatga tggaagagat
    23101 gaaaaagaac aatgccggat cgcttcactg aggtggcaat atgacagcac aaattgcagc
    23161 ttacgggcgg ctggtgggcg acccgctggt aaaacagagc agcaagggca caccggagcc
    23221 gccaacggag aactacgcag acctgagaaa tgcagtaaat cgcacttacg cagacatggg
    23281 gctgagcaaa ttagcagagt aggaatatca gaaccacacc acgcagccgg agcaatccgg
    23341 ctttttttgc gtcatttgaa acggtatgga gcactactgg cttttcgtca cggtccggca
    23401 tagttactat ctgaaacaaa cagacacaac agaggaaaac gcaatgccga tgaaatttga
    23461 tgagatactt aaacagcgtg ataaatacca tgctgacaac atggagacga tgaatatcac
    23521 tgattaccgc gcatttctgg aaacgggggc gctgattgaa aaggatcatc atggttttgt
    23581 tcgttgcgcg ctatccggtg aaatgctggc agtaaatcct gaacagacag atgcattgat
    23641 agaatttctg aaagggatca gagactgagc acccatacag cacatacagc cggagcaatc
    23701 cggctttttg ttattttttg taaattattt gttcgtggtt gttccacgtt gttcactgag
    23761 cggatcggca tattttaccc gaactgaatc atgattattc tcgcccgtgg tgccaggacg
    23821 ctggggccac tttcccgcct gttaatgtgc tcgccaatat tcattaccag gcgggaaaac
    23881 gatcggtgcg attactgatt tccttatgaa aaacggttga gtgtttgccg cgtcctggag
    23941 ttccttactt aaccccagga ctttttttat gcagagaata atcgaattac gccagcagaa
    24001 aaccgccatt aaaaatcaga tgcgcgacat gctggagaac gctgaaaaag aaaaccgcag
    24061 tcttaacgat gctgagggca caaaatttga cgaattacgc gctaaagctg aatccctcga
    24121 taaagacatt tcccgccttg aagccattgc agacgaagag cgcagcaagc caggtaaaag
    24181 cagccagacc actgaccccg cagaactacg caactacatt ctgaccggtg aaacccgcgc
    24241 attaagtaca ggcgttcccg ctgatggtgg ttataccgtt atccctgaac tgaacaccga
    24301 aatcatgcga atgctggcgg atgaatccac catgcgtcgc atctgtaccg tgaagaaaat
    24361 cagcagcaac gagtttaagc agcttgtttc cgctggcggt gcgaccgtta accacggtga
    24421 agagggtaag gcacgcgaac agaccagcac cccgcagatt aacgaggtga gcattaagct
    24481 gtatccggtc tatgcgtacc cgcgcaccac acaggaaatc gtggattttt ccgatgtgga
    24541 catcctttca tggctgacgg gtgagattgg cgacaccttc acggaaaccg aagaaagcga
    24601 tctggttgtg ggcgacggtg acaaaaaagc aaaaggtttt ttatccgtac cccgtgcaga
    24661 gaagaacgac aaagagcgtg attttggtac gttgcaggta attaaacctt ccgaatctct
    24721 ggcgtggaca tctgcggacc cgctgatcga cctgaaattt gcattacgta aaaaataccg
    24781 caaaaacgcg gtctgagtgg ttaactccac gacggcggca aaacttcaga aggtgaagaa
    24841 cgcgaacggt gattatatct ggcgcgaccg tttacaggcg ggtgatcctg atacgttgct
    24901 gggccttccg gtcgaatatc tggagtttat gcctgataac gttattgccc tgggtgactt
    24961 caaacgcggt tactacattg ttgatcacga aacaggtgtt cgcaccagac cggacaacct
    25021 gacagaacca ggattcatca aaattttcac gcaaaaatat ttaggcggtg gcgtggtgga
    25081 ttcgaacgcg atcaagattc tggcactgcc ggaagacgac gattaacagc atacagaagg
    25141 ggctaaaaag cccctttagt gttttatggg tgaaaaaatt atgaagagta tggaaatccg
    25201 gtcatcggaa atcaccacca gcggagccgg tacgctgacg ggctacgttg ttcgctggga
    25261 taagctttca gaactgttat ggggtgagtt ttacgaaaaa ttccagcggg gggcgtttac
    25321 tgaatggctt gcggcgggta atgacgttcg cggcctgtat gagcatgacc acagcatgtt
    25381 actggggcgc acccgttccg gcacgctgaa actggaagag gacgaaacag ggttacgctt
    25441 tgaactgacc ccaccggatc ccagtacagg gcgcgacgtt atcgaactgg ttaagcgtgg
    25501 ggacatctcg gggatgagct ttggctttcg ctcccgtaag gatgtatggg ataccacaac
    25561 agatccatgc gtgcgcaccg tgctggtggc ggaactgtac gaaattaccg ttacatcggt
    25621 accggcttac cccgattccg gcgtggagct ggcccgccgt tccctgtatg agcagcaccc
    25681 cgaaaaaatg ccgcgtgcgg ataatcgccg ctggtgggcg gatttagcgg gggtgtgata
    25741 tgtggccttt cagacgaaaa aaagagcagc gcagcatgac gcttgatgaa tttatggcgc
    25801 tggccggcac atcgaacacg ggggcgggtg agtacgtatc atctgggaca gcggaatcac
    25861 tccccgccgt catgaacgcc gtcacggtca tctctgacgc ggtggctacc atgccgtgtt
    25921 acctgtacct ggtacgcaat gagaagggga aggaggcccg cgaatggctt gattctcatc
    25981 cggtcgatca catcctcaac gagcgcccga acgcgtggca gacaccctac cagtttaagc
    26041 gaatgatgat ccgctactgc ctgttaagtg gtaatgctta tgcggttatt cagtgggggc
    26101 gtgatggttt tccggcggct ttacatcctt acccgccgca gtcggtgaac gtggatcaga
    26161 caggtgatca taactggcgc tactgcatca ctgacgccta caccggaaac acccgcaact
    26221 atttaccgtg ggaggttctc caccttcgtt actccacgga tgacggtttt atggggcgct
    26281 cacctgtaac catctgccgc gaatcgctgg ggcttgggct ggcccaacaa cgccacggcg
    26341 cgagcgtgat gcgtgatggc atgatggcgg caggggttat cacgtcaggc gaatggctgg
    26401 acggcgtgaa gggcaaacag gcattagccg cactggaacg ctacaaaggg gccagaaacg
    26461 ccggaaaaac gcccatcctt gaagggggga tgagctatca gcagctgggc atgagtaatc
    26521 aggatgccga atggctggcc tcccgtcgct tcaccattga agacatcgcc cgaatgttca
    26581 acgtctcgcc gatttttttg caggaataca gcaacagcac ctacagcaac ttcagcgagg
    26641 caagccgcgc atttctcacc atgacgatgc gcccgtggct ggcgaacttt gagcagcaga
    26701 taaaaaacgc cttgctggtg gcctcgcctg tacctggtat ccggtatcag gtggagtttg
    26761 acagcgcgga cctgttacgg gccacacctg gcgaacgctt tgccacctat gagcgcggca
    26821 taaaatccga cgttatgtgt ccgaacgaag cccgcgaacg tgaagggctg tcaccgcgtg
    26881 atggtggtga tgagttcagc caggcatgga aacaggaagt aaaaatcagc gagggagaaa
    26941 aaccggaatg aacatagggc gactgcgtga caggataacg attcagaccc tgaaacagac
    27001 cagggatata accggcgaaa tactcgaaac gtgggaggac ggtcacacac tctgggcaag
    27061 cgtgaacatg gtcagcagca aggaggccat ttcatcgggc gcagagctgg cgattggtac
    27121 cgtaaggatc tggatacggt accggaagga catcaacgcc accagccgga taaaggtcag
    27181 tacggggccg ctggcggggc gtgtactgaa tatcatcggg cagccgctgc cggatgccgc
    27241 caggacacgc cttgaaattc tttgtcgtga gggcgcggaa aaatgacaga agaacttatc
    27301 accctggaag aagtgaaact ccattgccgc atcgatggcg acgatgaaga ccagttaatc
    27361 agcggataca ttgccgcatc gcttgaggcg tgccagatac acataggcag gcgctttgat
    27421 gacgggctgg cgttcacgcc agccataaag attggctgca tgatgtttat cgctcacctg
    27481 tatgaaaatc gccagattgt cgcggataac gcaaaaacac gcgtacccat gacgataggc
    27541 gcactctgga cggcttaccg tgatgtgggg gggtactgat gccgtggcaa ccattaagac
    27601 gatgcacaga accaggctgt aacaggcgcg ttaaatccgg caagtgtgag gagcacaggc
    27661 gggcggcatg gcgtgcagag gatgccagac gaggacaccg ccgcgcgcgt ggatattcca
    27721 gacagtggga caaataccgc gccatgtatc tgagtaaaaa cccgttatgc gtgcgatgcc
    27781 ttgagaaggg gatatatacg cccgccgtgg tggtggatca cattatcccg attaatggcg
    27841 gtgatgatgt tctcttctgg cccgaatgga accatcaacc attgtgccag gcgtgtcata
    27901 accagaaaac gaaatggctt gatccggcaa caaaaagcaa gcgtgccgca ggtggatttc
    27961 gtgaagagga agaacgggct gctaaccgca ataactggat gtatgacgct gatgaatgag
    28021 cgggaacaaa accgccttat ccgtggactt ataaggcagc gtgacgcatg gaagacacag
    28081 gaaacagggc ataaagataa agcgtcagga cgcgcagaac gcatcacagc gaagcgatta
    28141 accgaccgtg accgcgaggt tatggaatgt ttccgcaatc gctgatgagg ctgtatgacg
    28201 gggtgggggg cgttttcagg acaaacccga ccccgcctac ctggatgata tcgccgtgaa
    28261 gcagtggcgg gaaaaatcgc ggcagcttgc ggaacgggga gacctgaccc ccgccgactg
    28321 gagcaatctg gaactgtatt gcgtcaacta ctccatttac cggaaagccg ttgcagacct
    28381 tgcggcgcgc gggttcagca ttgttaacag tcagggcggc gagagcagaa accccgcatt
    28441 aagcgcaaaa tccgacgctg aaagagtgat gataaaaatg gcctccttgc tcggttttga
    28501 cccgataagc cgccgtaaaa atccaccgga aacagaagaa gaggacgagc ttgaccgcct
    28561 ggaataagta cgcagaagac gtaaaaacgg gcaaaattcc ggcctgtaaa cggttaaaac
    28621 aggctgttaa acggtacttt tcggacctta aaagccccct ttacacgttc gatcgtgagg
    28681 ttgtggagcg gtttattgcc ttttccaggg tgtgcccgca cgtaaaagga ccgatgcgtg
    28741 gcagacccat tgagctggag ccgtggcagc agttcgcctt tgcgtgcatc ctcggcttta
    28801 aggttaaggc caccggacgg cgcaaataca cgagcgcctt tatcgaagtg ccgcgcaaaa
    28861 atgcgaaatc cacgaccgcc gcgattctgg ctaactggtt tctgattatg gagaacggtc
    28921 agcaggatat ctacaccgca gcggtgagcc gtgaccaggc gcggatcgtg tttgataatg
    28981 cgcgtcagat gtgcctttta tcccgaccgt tacgcaggcg ggtgaatatt caggcgcata
    29041 aggtgataca cccgaaaagc aacagcctgt taaagccgct ggcagcaaaa gcggcaacca
    29101 tcgaggggac aaaccccagt ctttccatcg tggacgaata ccacctacac cccgataacg
    29161 gcgtttattc cgcgcttgag ctggggatgg gcgcacgtcc cgaagggctg ttatttgcca
    29221 ttaccacatc gggcagtaac gtcgtttcag cctgtaaaca gcactatgat tattgctgcc
    29281 agatcctgga cggcgaagag gtcaacgatt caatttttgt actgatttac gaactggacg
    29341 acgaaaacga ggttgatgat ccggcgatgt ggataaaggc gaaccctaat attgatgttt
    29401 ccgtggatcg tgaaaaactg gcctcaacca tccagaaagc gcggggtatt ccgtcgcagt
    29461 gggtggagat gatgacaaag cgatttaata tctggtgtca ggggactacc ccgtggatgg
    29521 gtaatggtgc atgggctgag tgtgccggaa cgttcacgga ggaagattta cacggtcagg
    29581 agtgttacgc ggggctggac ttatcatcaa ccagcgatat ttccagcgta tgctatgcct
    29641 ttccggtcgg taaaaccatt atgctgattt cccgtcatta tctgccggag ttccagctac
    29701 agaaccccgc caataaaaac cgcgctgtct atcgccagtg ggctaaagcg ggctggatac
    29761 gcacaacgcc aggtgactgc attgattacg accggatcag agatgacatc atgcaggatg
    29821 cggagaaatt taatatcagg ctggtgggct ttgatacgtg gaacgccacg catctcagga
    29881 cgcaattaca gggggctggt tttgaggtgg agccgttccc gcaaacctac ctcagattca
    29941 gtccggcggc gaaatcgttc gaagtttttg ttaaccgcag ggtgattgtg catcgtggcg
    30001 atccggtgtt gtcctggtcg atgagtaacg tcgtgatgca gagtgacgcg aacgccaata
    30061 tcaagccgaa caagaaaaaa tcaccgaaca agatagaccc gagcgtagcg gcgctgatgg
    30121 cgtttggtac attccaggca gagcatgagg attttgcttt tgatataagc gacagccacc
    30181 gccagaaact ggaagaattt agcggggtgt aatgaggtca gcagcatgac agaagccgaa
    30241 atactgcgat taatccgccg cgtctctgga atcagccagc aggctgacga acagaccacg
    30301 cagccggaca gcgtgacagc cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt
    30361 gatggtatcc agcttaatga tgcggatatg cgcgacatac ggatccgcgt tctggaaatg
    30421 ctggcctaca atcgccgcgt tgaactgtat cgggagaagg agaaaatcac gtaccactgg
    30481 aagaagccgg agcggttacg gcggtaactc actgagatta aagcaaagcg caaaattgcg
    30541 ctggctacat ccctcacatc tgaggactgt taaccagcag gccggaatat attcagaaac
    30601 tacgaaattt tcgcagttta attactcgtc agcttaatcg ctaacccgct ctgatatttt
    30661 cggaaacctc aatttgagga agtcggcgag gtaactgttt gatattcccg atagcgcaaa
    30721 aatgcgcggt cgggcagaat gaccaccacc tcaatcgtga cggagagtga caaccagtga
    30781 cacaccgtga caaaaagacg gtttaaccgt tcattaaaaa tcgacgtgat tttgtgcttt
    30841 ccggatgacg tggccaacgt cattttttag cgaaaaattc tgattaaggt cactggctga
    30901 acggtcataa tgaccacgca gaccatcgag gcaaccatag cgatcatcgt tacggttgga
    30961 gcttttccgc cattggtgga aaaggtgcac atctggatca tcattacggt gatcaagggt
    31021 atgtaaaacc caccagcctg attaaaggtt aaccggaaaa aaaatccggt agccccgtta
    31081 tggggatccc catatcgaca ttaacgcccc tcatgaattg gtgcgcttcc ccctggaaag
    31141 attacccgcc tgtatatttc ttgtgtctat ttgttccacg ttgtttcata cagtgcaccg
    31201 aacggtgtag ttaatggtgt agtcaatttg cgatttttgg cactttttca atgaaaattg
    31261 tttgatcgaa tcaatgactt gatgtagtgg taaaaggaca tatttattca tctttcggcg
    31321 cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc gcccagcatg ctctctccgg
    31381 cgacgatttt accctcatat tgctcggtga tttcgcgggc taccgccagc cctacacctt
    31441 gcccagggcg taaagtatca acccgttgac cacggtcgaa aatgacctct cgcttgctta
    31501 atggaatacc ggggccatca tcctcgacca caatatagag atgctcgtcg gtttgccttg
    31561 cagaaatttc gacaaactcg aggcaatatt tacaggcatt atccagcacg ttgcccatca
    31621 cctcgacaaa atcgttctgc tcaccgacaa agctgatctc tggcgaaata tcgagagaga
    31681 tattgacccc tttgcgttga tacactttgt tcagcgctga ggtgagattg tccagcagtg
    31741 gggcgaccgg atgcagctcg cggctgagca atgtcccgcc gcgcatactg gcacgatgca
    31801 ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc cagcattacc ggctcagcat
    31861 cactgacgct catcttttca ctacgcagag aacgcagcgt actttgcagc accgccagtg
    31921 gcgttttcag actatgggtc aggtcggtga gcgtcgtacg gtatttgtcg taacgttcgc
    31981 gttcactttt taacaatcgg ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg
    32041 ctggattgag caattcgcgg ttatgttctt ccagttcgcg gacttctttt gccagggctt
    32101 cgatggggcg taaactccac caggcggcga cccacagcag cgggatcact aacagcagat
    32161 tggctgagag cacatagata aaccagctcc agaccatata ggaacttttt agctccaccg
    32221 gaatggtatc caccaccaca atggttaatt ttggcatccg cgatgttgcc gggtagacgt
    32281 ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact tcctgcaact
    32341 gttgctgtat cgaatgatct ccactcagca agaggctggt atcgttaaca tccgcttcaa
    32401 tttcatgaaa accattcgat ttcagccagt caggctggat cattttcatc agccagggca
    32461 cgtcacgttg cgcccataaa agctgcccgt tctcatcata aattagcgtc atggtggggc
    32521 tttgcttgtc gatattttcg ggtaactcga catgcaactt attgttttcc cactgcgcaa
    32581 gggtatagaa cagattgctc tcgccacgta acagccgaaa cgtagtttta tcgaaactga
    32641 cgctataacc gatcagcgcg accattccgt aggcaagcga aagcaccagt actaccgctg
    32701 ccgttgccaa cagaaaacgt acccgcagcg agagcgggaa aaaaagacgc agtaattttt
    32761 tcatcagcgc aattcgaaca ggtagccctg gccgcgaacg gtggtaatga cttcttgcgg
    32821 atattgtgcc tgaatttttt tgcgcagacg tcccatcagt acatcaatgg tatggctttc
    32881 ccgcagctcc gcatccggat agagttgtag cattaacgaa tctttgctga ccactttgcc
    32941 attattgcgt atcaacgttt ccataatggt gtattcgaac gcggtcagtt tgatcacttc
    33001 gtcattaata gataattcac ggcgagagag atcaacctga aacgggggga gcgaaatgac
    33061 ctgtgaagcc agaccgctat tacgccgcat taatgcctgc attcgcgcca tcacctcttc
    33121 aatatgaaac ggtttagtca cgtaatcatc agcaccggca cttaatactt cgactttgtc
    33181 ctgccagctt tcacgggcgg ttaataccag aatcggcagt gaaacatcgt tgctacgcca
    33241 gcggcgaatc agtgacagac cgtcctcgtc aggcaatccg agatcgacaa tcgcaatatc
    33301 cggtaaatgt tcattgagat aataatcggc ttctttggca tcttctgcgt catcgacctg
    33361 atgaccagca tcctgaatct gaactttaag gtggtgacgt aacaacgcat tgtcttcaac
    33421 aaccagtacg cgcattttta tttctccctg tcttaattat taaaatagtg taacgcgatt
    33481 atgtgtttat gggggtaaac attaaataaa ccagcgggga ggggaggtaa agtgaaaaaa
    33541 gaaaaagtgg ataatcttaa taagcaggcc ggacagcatc gccatccggc actgatttga
    33601 ggcttatttc agctcatcaa ccatcgtgat agctcgacca atatagttag ccggcgtcat
    33661 cgctttcagg cgggctttct cttcttctgg caacgccaga ccatcgataa actgcttcat
    33721 gccttcggcg tcaacgcgct taccgcgagt aagctctttc agcttctcgt acggtttttc
    33781 gatgccatag cgacgcataa ctgtctggat tggttctgcc agcacttccc agttgtgatc
    33841 cagctcatcc agcagatggt cacggttcac ttccagtttg ctcacgcctt tcagggtgga
    33901 ttgatacgca atcagcgcat aaccgatacc cacgccgagg ttacgcagca cggtggagtc
    33961 ggtcaggtca cgctgccagc gggaaaccgg cagtttgctt gccagatgct gcaataccgc
    34021 gttggaaagg cccagattcc cttcggagtt ttcgaagtcg atcgggttaa ctttatgcgg
    34081 catggtggaa gaaccaatct caccagcaat ggttttctgt ttgaagtggt taagggcgat
    34141 ataaccccag acgtcacggt caaagtcgat cagaatggtg ttgaagcgcg caacgcaatc
    34201 aaacagttcg gcaatgtagt cgtgcggttc aatctgggta gtgtacgggt tccattgaat
    34261 acccagcgag gtgacgaact cttcgctgaa ctgatgccag tcaacttccg ggtaagcggc
    34321 gatgtgggcg ttatagttac cgaccgcgcc gttgattttg ccgaggatct ccacctggtt
    34381 aagctggcgg tgctggcgct ccatacggta ggcgacgttt gccatctctt taccgatggt
    34441 tgacggcgtg gctggctgac cgtgggtacg agacagcagc gggatatcgc ggtactgaac
    34501 ggcgagatct ttaatgccat caatcagttg acgccagtac ggcaggatca cttcatcacg
    34561 cgcggttttc agcattaatg cgtgggagag gttattgata tcttccgaag tacaggcaaa
    34621 gtggatgaat tcagaaaccg cgtgcagttc cgggatctcc gccacttttt ctttcaggaa
    34681 atactcaacc gctttaacgt cgtggttagt ggtacgctcg atggttttga tgcgtgcggc
    34741 atcttcttcg ctgaaattgg cgacaattgc atcaaggtaa ccgattgcgt cggcagcaaa
    34801 agcaggaact tccttgatcg ctgcgtgcgc ggccagtttt tgcagccaac gtacttcaac
    34861 ttgtacacgg aatttcagca aaccatattc gctgaaaatc ccgcgcagcg cgctgacttt
    34921 atcgccgtag cgtccatcga caggggaaac ggcggtcagt gaggataatt ccatagatca
    34981 caactccggg gttaaatgag caagaatttg ttttgcctga gtggtcaggc gattacgaga
    35041 aaacatcagt tgtagacgtc cgccgccgac ctggtgccag agcacggcgg cgcgaatgcc
    35101 tgccagcagg gttgcgcgaa ctttcgcctg cacttgtggg ctttgcagta cagcagggga
    35161 accggtgacc tgaatgcgcg ggccaagcgg gctgatcaca tcaacataga tagcagccat
    35221 cgcgctcatc agcgtttcgg actgtaaatc gaagtgttcg agctggcgtt gcaggccgtt
    35281 gatccggttg cccagagtgt cgagcgcgcc tttcgctgag gagagtttgc gctcaagcac
    35341 catcaagctg agtgtgtagc gggttaattc ggcgtttaag ccctggcaac tgctggcatt
    35401 gagcacgccg agcagggttt ccagcccgac gcgcaggttg gcttcgctac cgccaaaaac
    35461 cgccagcgtc gagctggggt tcatatcaat aatactgttg agtgagacgt gtagcgcatc
    35521 ggcatcacaa tgcccctggt gagcgagttg ttgcaccagg cgtgccgact gacaaatacc
    35581 ggccagggcg agggtgatgt catagtaatt ctttgccacg ttcactgctt ccttgtttta
    35641 agtaaagata ataatcagac cggcagcggc agacgctgct caataatacc gccaccgagg
    35701 cacacttcac cgttatagaa gacggcagac tggcccggcg tcacggcggc aaccggctca
    35761 tcgaaaatca ctttaatgcg atcgtcgtcc agcgccttga cggtgcacgg aatgtcggtc
    35821 tggcgatagc gggtttttac cgtgcaacgc atagtgccgg tgaatggttc gcgatcgacc
    35881 cagtgcagct gctgggcaat caacccgaca gacatcagcc gcgggtgttc atggccctga
    35941 gcgacaacca gaatgttgtt ttcgacgtct ttgtccacca cataccacgg ttcttcggtg
    36001 ccttctttgg tgccaccgat acccagacct ttacgctgac cgagagtgtg atacatcagc
    36061 ccctggtgct caccaatttc atcgccatcg acggtaatga ttttgcccgg ttgcgccggg
    36121 agataacggc ccaggaactc gcggaattta cgttcgccaa tgaagcaaat gccggtagag
    36181 tcttttttct tcgcggtgac cagaccaaga tcttcagcaa tcttacgcac ctgcggtttt
    36241 tccagttcgc ctaccgggaa caggctttgc gcaatctgct catggctgag cgtataaagg
    36301 aagtagctct ggtctttatt gctgtccaga ccacgcagca ggcggctctt gccatcgaca
    36361 tcggcccgac gtacgtaatg accggtagcg atataatcgg cacctaaatc ttcggcggca
    36421 aattcgagga aggctttaaa tttgatctcc ttgttgcaca gaatatccgg attcggcgtg
    36481 cgaccggctt tatattcggc aaggaacagt tcgaagacgt tgtcccagta ctcggcagca
    36541 aagttaacgg tgtgcagttc aatgccgagc ttgtcgcaga ccgcctgggc atcagccaga
    36601 tccgctgccg ctgtgcaata ttcctcaccg tcgtcttctt cccagttctt cataaacagg
    36661 ccttcgacct gatatccctg ttgttgcaac agccaggcag aaacggagga atcgacaccg
    36721 ccggacatgc cgacgattac tttttttgcg gtttcagaca ttggatcact cacgacattg
    36781 aacttcaagg cggcgtattc tatcacgcag ccttttagat gacacccttt gtaaaaggcc
    36841 agttaaaatc gccaatcatc tccagcggat aacgttgccc gctttgataa caacgaatac
    36901 tttccgccac cagcggcgaa cgaagatttg acgcctgtaa aatttcttcg gcgctgaccc
    36961 aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg gcatatttgc tcaagctcaa
    37021 tggcaaagag gaaacgcaaa aacggcgttt tatctggcgc aatccactga tgcatacgaa
    37081 taaagtgttg cggctgcgcg ctgatgccgg tttcttccca cagttcacgg gcggcggctt
    37141 ccactaaggt ttcatcggct tccagatgcc cggcaggttg gttccataac gctttaccat
    37201 taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg caccacgcaa gcaacggtaa
    37261 cgtgcggttt aaacattttg catccttaat ctgtcacttc tcgccattcg ccattggcaa
    37321 gattatccaa agagtaatct cccatcgcat agcgaatcag tcgcagcgtg gggaaaccaa
    37381 catgggcggt catgcggcgc acctggcgat tacgtccttc atataaggtg atcttcagcc
    37441 agctggtggg aatacttttg cgttcacgaa ttggtggatt ccgcggccat aaccacgcgg
    37501 gttcgtcaac cagctccgtg cctgcgggca gggtagggcc atcatttaag gttacgccat
    37561 tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc cacctgcaca taatagattt
    37621 ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa cgcgccgtta ttggtcagca
    37681 ccagcaaccc ttcgctatcg cggtcaaggc gacctgctgc ataaacgccc tgaaccggga
    37741 tgaattcttt taatgttttg cgtccggttt catcggtgaa ctgcggaaga acatcgtagg
    37801 gtttattgaa gaggatcaca cgcgtgggct ggttttcatg tttacgcctg gtagaacgtt
    37861 gcgagctgaa tcgcttaacc tggtgatttc taaaagaagt tttttgcatg gtattttcag
    37921 agattatgaa ttgccgcatt atagcctaat aacgcgcatc tttcatgacg gcaaacaata
    37981 gggtagtatt gacaagtcaa ttacaaatca ttaacaaaaa attgctctaa agcatccgta
    38041 tcgcaggacg caaacgcata tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa
    38101 ggagaggtga atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca
    38161 aaacggcaaa ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat
    38221 cggtgtagat gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta
    38281 taaaggcgag cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt
    38341 ttatggtcag gatgtctggc tgcctgctga aacccttgat ctgattcgtg aatatcgcgt
    38401 tgccattaaa ggcccgctga ccactccggt tggtggcggt attcgttctc tcaacgttgc
    38461 cctgcgtcag gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac
    38521 tccaagcccg gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga
    38581 agacatttat gcgggtatcg aatggaaagc tgactctgcc gacgccgaga aagtgattaa
    38641 attcctgcgt gaagagatgg gcgtgaagaa aattcgcttc ccggaacatt gcggtatcgg
    38701 tattaagccg tgttctgaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc
    38761 aattgctaac gatcgtgact ctgtgaccct ggtgcacaaa ggcaacatca tgaagttcac
    38821 cgaaggcgcg tttaaagact ggggctacca gctggcgcgt gaagaatttg gcggtgaact
    38881 gatcgacggc ggcccgtggc tgaaagttaa aaacccgaat accggcaaag agatcgtcat
    38941 taaagacgtg attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga
    39001 tgttatcgcc tgtatgaacc tgaacggtga ctacatttct gacgctctgg cagcgcaggt
    39061 tggcggtatc ggtatcgccc ctggagcaaa catcggtgac gaatgcgccc tgtttgaagc
    39121 cacccacggt actgcgccga aatacgccgg tcaggacaaa gtaaaccctg gctctattat
    39181 tctctccgct gagatgatgt tacgccatat gggctggact gaagccgcag acctgattgt
    39241 taaaggtatg gaaggcgcga tcaacgccaa gaccgtaacc tatgacttcg agcgtctgat
    39301 ggaaggcgct aaactgctga aatgttcaga gtttggtgat gcgatcatca agaatatgta
    39361 atcactacat gtgttaaata tttaaacggg cgtataacac gcccgttgtt ttatttatgt
    39421 ggatattatt aatagcatat cgagcatatt taggtaatga ctccaactta ctgatagtgt
    39481 tttatgttca gataatgccc gatgaccttg tcatgcagct ccaccgattt tgagaacgac
    39541 agtgacttcc gtcccagcct tg
//
LOCUS       DAECOD010000025        38434 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.025, whole genome shotgun sequence.
ACCESSION   DAECOD010000025 DAECOD010000000
VERSION     DAECOD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.025"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..74)
                     /locus_tag="KKO15_001379"
     CDS             complement(<1..74)
                     /locus_tag="KKO15_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBD6360227.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KKO15_001380"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KKO15_001380"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBD6360228.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KKO15_001381"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KKO15_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBD6360229.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KKO15_001382"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KKO15_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBD6360230.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KKO15_001383"
     CDS             2704..2940
                     /locus_tag="KKO15_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBD6360231.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KKO15_001384"
     CDS             2943..3800
                     /locus_tag="KKO15_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6360232.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="KKO15_001385"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="KKO15_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="KKO15_001386"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="KKO15_001386"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBD6360233.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="KKO15_001387"
     CDS             complement(6415..7311)
                     /locus_tag="KKO15_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBD6360234.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="KKO15_001388"
     CDS             complement(7360..7599)
                     /locus_tag="KKO15_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBD6360235.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="KKO15_001389"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="KKO15_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBD6360236.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="KKO15_001390"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="KKO15_001390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBD6360237.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="KKO15_001391"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="KKO15_001391"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBD6360238.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="KKO15_001392"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="KKO15_001392"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBD6360239.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="KKO15_001393"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="KKO15_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="KKO15_001394"
     CDS             complement(15268..16476)
                     /locus_tag="KKO15_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6360240.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16484..16984
                     /gene="tnpA"
                     /locus_tag="KKO15_001395"
     CDS             16484..16984
                     /gene="tnpA"
                     /locus_tag="KKO15_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBD6360241.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="KKO15_001396"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="KKO15_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBD6360242.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="KKO15_001397"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="KKO15_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBD6360243.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="KKO15_001398"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="KKO15_001398"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBD6360244.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="KKO15_001399"
     CDS             complement(19387..20556)
                     /locus_tag="KKO15_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6360245.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="KKO15_001400"
     CDS             complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="KKO15_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBD6360246.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="KKO15_001401"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="KKO15_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBD6360247.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /locus_tag="KKO15_001402"
     CDS             complement(22132..23064)
                     /locus_tag="KKO15_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6360248.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /locus_tag="KKO15_001403"
     CDS             23177..24388
                     /locus_tag="KKO15_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBD6360249.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="KKO15_001404"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="KKO15_001404"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBD6360250.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="KKO15_001405"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="KKO15_001405"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBD6360251.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="KKO15_001406"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="KKO15_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBD6360252.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="KKO15_001407"
     CDS             complement(28137..29393)
                     /locus_tag="KKO15_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD6360253.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="KKO15_001408"
     tRNA            29701..29777
                     /locus_tag="KKO15_001408"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="KKO15_001409"
     tRNA            29782..29858
                     /locus_tag="KKO15_001409"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="KKO15_001410"
     CDS             29966..30271
                     /locus_tag="KKO15_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBD6360254.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..32001
                     /locus_tag="KKO15_001411"
     CDS             30397..32001
                     /locus_tag="KKO15_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBD6360255.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(32013..32825)
                     /locus_tag="KKO15_001412"
     CDS             complement(32013..32825)
                     /locus_tag="KKO15_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360256.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32829..33614)
                     /locus_tag="KKO15_001413"
     CDS             complement(32829..33614)
                     /locus_tag="KKO15_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBD6360257.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33611..34279)
                     /locus_tag="KKO15_001414"
     CDS             complement(33611..34279)
                     /locus_tag="KKO15_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD6360258.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34343..34981)
                     /locus_tag="KKO15_001415"
     CDS             complement(34343..34981)
                     /locus_tag="KKO15_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBD6360259.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34994..37095)
                     /locus_tag="KKO15_001416"
                     /pseudo
     CDS             complement(34994..37095)
                     /locus_tag="KKO15_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37116..37742)
                     /locus_tag="KKO15_001417"
     CDS             complement(37116..37742)
                     /locus_tag="KKO15_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBD6360260.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38198..38407)
                     /gene="fumD"
                     /locus_tag="KKO15_001418"
     CDS             complement(38198..38407)
                     /gene="fumD"
                     /locus_tag="KKO15_001418"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBD6360261.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9256 a        10038 c         9413 g         9727 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccgac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctgggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccgcaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acagcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacggcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgtttgaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatggc gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctggcctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acgacgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatcattcat
    31621 attctcgccc tcggtgaaga ctacgaaatg gaaattaatg agtcgcgcac cgtcctgaaa
    31681 acggaagaca acagctactc gtttgacgtt tttattgatg cccgcggaca gcgtccgctt
    31741 aaagtgaaag atattccttt ccctgggcta cgcgagcaat tacagaaaac aggggatgaa
    31801 atccctgatg ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta
    31861 gcgttcggcg cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca
    31921 tgtgcagaaa ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt
    31981 cggcgtcttt cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca
    32041 ttaagacgat agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag
    32101 cgaactcttt cgccgtcatg atatgccagt gcggcgcttc cccctagccc gagaataatt
    32161 gagtcgacca cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca
    32221 ttgtcgccgc tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca
    32281 ccattactgg cgacatgaca ccgttcaccc aatgtacaaa cgcgtactcg cattccggta
    32341 ttcgcaatcc gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca
    32401 acccgtgcgc gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc
    32461 acgctataac ccatactggc aatttgcgcg gcatacccag cggttgcgat tcgggcgtta
    32521 tcctcacaac agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt
    32581 atctctccgg ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt
    32641 tcatccgcca gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat
    32701 ccttcctcaa tcagtgcatg acgaatatcg gcataactac cgccgcgtgg gaaatggcgc
    32761 aggaaccagc gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg
    32821 atcattcgtt agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta
    32881 cgccccgtgg tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga
    32941 gcaaaatgcg cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc
    33001 ggatagagac acaacagccc cgtcaatagt aacaacggca gcaatccata catgacacca
    33061 acataggcga cctgctgtaa gggattaaat ttagactgtg ttgttgccgg gaaaggatgt
    33121 tcttccccct gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgttccagc
    33181 cacccctgac gacgaatgcg atagtggtga ccattatccc caacggcatt gatcagcaca
    33241 aagccgagcc agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt
    33301 ttaaccgcag ttgcgcccac catcgcaaag tgatttatca gcccacttgc cagcaacaat
    33361 acaaagagta acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaacc
    33421 ttctcaccat gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc
    33481 cccagcacca accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa
    33541 aattcgggag tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt
    33601 tgcgacgggt tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat
    33661 gcggttttcc ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct
    33721 gaatttctgg gctatcttcg cgaccaaaga tcaatgcgtg ttccggacag gcgctgacgc
    33781 aaatgggcgg aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca
    33841 ctttagtgac agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc
    33901 agccgatgca ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgatgccc
    33961 ccgtcgggca tacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa
    34021 agtgatactg cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc
    34081 ttccctgagc ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat
    34141 tgcaccgtga ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg
    34201 ctaacagaga agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt
    34261 tgcgtcgagt gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta
    34321 tccatgacat ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc
    34381 ataagccata agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg
    34441 tcgatgactc cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg
    34501 cggcttcaat ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt
    34561 aaggacgagg atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt
    34621 cctgctccac aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt
    34681 aatagtaatc atcctgactt cccgtcatac aagcgatatc cgcgtagtct ccgttctgct
    34741 taatgctttc cagcaaaact gacagttcct cttctgcaac cgaatagggt ggcgagagaa
    34801 acacggtgcg cgccaccagc tgcccagccg cagaatgctg gcgaatgaac tcagccagga
    34861 tttcagcagg ttctggttcg gttaccgtat caacggggtg acgcatcccc tgcaaccatt
    34921 gccgcttcgc ctcgtcaact tcgctgactt tcgctaacgg caattcatcc tgatggttca
    34981 tcttacccat tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt
    35041 ccagccccag acgctgcaat gtttcgcggg ttggacaacc aagctgtggg tcccagccca
    35101 tttctttgta gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac
    35161 cttcagtaaa gaccgggatc tgcggatcct tgtcgaaaac ccaggaacag ataagatcgt
    35221 gttcattacg catatctttg gtttgcatcg gttttaccgt gtaggcacga tgcaacgtaa
    35281 agatacgctc tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt
    35341 tgaagaattt ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa
    35401 cggtcattgg ccagacccag ttgcacagag tgacggcgtt atgcaaacag acccgcaaca
    35461 gcgaccactt tgcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat
    35521 cgtaagcatc ttcagaacca aaaagttctt tcgccacttc acgttgcagt ttcaatggca
    35581 agccggaacc aataaagttg atatgggtat gcgtcatgca gtcacggttg aacatacagt
    35641 taacaatgga accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg
    35701 gcgaccagag tttatttttc gcgtagcccc agtactcttt acccaaattc cagcgttctg
    35761 cgatggcata tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt
    35821 agtaaaaatc tttaatgaag ttaacgtcac ccgcttccag ttgatcccag cgaatttctg
    35881 catactcttc agctggcaga acacgcttga acacaccttt gctgtaacaa taagtaaagt
    35941 cgcgatgcaa ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa
    36001 ccaggttacc aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga
    36061 agatggtggt atggacaaag tttgctacac aagtgttacc acctgtgctg ggcacgccaa
    36121 actctttcac ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc
    36181 cgctcatttt tactgtgtat ttctctgccg ccggtcctaa gtcaaaaacg gatttatagg
    36241 tacgaaagcc gaccgtattc tgattgcctg gcggaatttc acccgtttca atcggaccac
    36301 cttcagccgc gccccaaaac agccctttac gtgctgtcca gcgtgacttg ggatctgaat
    36361 actctgccca cgattgtggc gtacttggca cgacatggtt gttattcgca ccaataagtt
    36421 cagtcatcat gtaatcattg agacgcttca tctcctgacg atcggcaatg ttgacccctt
    36481 tcgtcccttc aaccgcaatc gctttcaggt ttttcgaacc cattattgcg ccagttcccg
    36541 caccgccgct gtggttacgg ctattcaaca tgccagagag aggaataagg ttttccccag
    36601 cctgaccaat agccgccaca caggtttccg ggctggtcaa tcgacaaatt tcttccgtcg
    36661 ttgcgcgcgt cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt
    36721 taatcttcag ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag
    36781 cgaatttcat ttgcgctgca aaaaagccac ccatatgggc atcgcgacta aatttccttt
    36841 ggtaaaagta gaaagtgagg tgatatttac gcgagaacta cagggggcac cagatccagt
    36901 taatgggccg gtagcaaaga ctaatttatt cgcttcatcg aaaggtttcg tgcctagcgg
    36961 tacttcgtca tacataattt tgtagccgaa gcccatgcca ccgacaaaac ttttaaactt
    37021 actggaatct tcgagggtaa tatttcctgt cgtgagattg actcttaata tattacctgt
    37081 ccaaccgtta gccatgattt tttcctttgc aagattcaca cagtaatatc tttccactcg
    37141 ataattttta acgcccctgt tgggcaggcg tttgcgcatt caccgcataa cacacatttc
    37201 gaggattttt tactttcggt atttacggtg gccatcatcc acgggcatgc cgtagtacag
    37261 gcgctacagc caatacaacg tttatgatcg acggtaatac agccttcttt ctgctgccag
    37321 gtaatcgcac caatcgggca gacgttcatg cattgcggct ctttgcattg acggcaggtg
    37381 tccgcggtat agttgagatc gccatacagg ccgccgccag agccaacccc gttgtcgcca
    37441 aagaaataat tgcgatggat tttgatacgg gagaagaatg tccctactga gccatcgttg
    37501 aagttggtac aagagatttc acagcggtgg caacccgtgc atcgcgctcg ctgggtcacc
    37561 agcacccctt tgggtgtatt tatcaaccct actgtgccgc tatcaatatc ttcctgtttg
    37621 caacccaaaa gtgacagtaa cgcaggggca attgttagac cagcaaggcc ttttcctgat
    37681 atacgcaaga attctaatcg cgttaagcca atatctaata gtggacgatc aaccgggttc
    37741 atttatttcg catcctcttc gcaaacacag tgctgacaat tcagcagcct gatattaagc
    37801 attcaactaa tacgctgctg actctgtccc caacaccaga tattttccct aacccgatag
    37861 tgtatgagaa aagatttaac gccgataaac taatccttta tggttaggag aaaaaataat
    37921 gtgattattc tgagccctta acattgatcg ttatcaatta aaattacaaa gcaagaaatt
    37981 ataagcgaac tgatatttat tatcatttga aataaattta acttaagcga caatcatttg
    38041 tgacgttcat tgcagaaaat aaaaataaca ttatcgctat aaattaaaat acataccgaa
    38101 attcgtataa tttcagcagt tgttatttag aatgattaac tggaccgcaa ctgaagcaag
    38161 aatatcagga tgaagcttac cgcctcatcc tgaatggtca ttttaccagt gcgttaatga
    38221 cggtttccat cgcctgcttt tccagctcac tgcgctgaat gcgtttattg gctaatgctg
    38281 tacgtaatac gcctgcgata acaatatgct ttggttcctg ccccagatcg cgcatttcca
    38341 gcacaacttt accgacaact ctgcacattt ctcggtacag ttcatcctct tttgtccgat
    38401 ttcccattct ttatacctat ttatcatgcc aact
//
LOCUS       DAECOD010000026        37627 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.026, whole genome shotgun sequence.
ACCESSION   DAECOD010000026 DAECOD010000000
VERSION     DAECOD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.026"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..175)
                     /locus_tag="KKO15_001419"
     CDS             complement(<1..175)
                     /locus_tag="KKO15_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBD6360262.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="KKO15_001420"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="KKO15_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD6360263.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="KKO15_001421"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="KKO15_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBD6360264.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="KKO15_001422"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="KKO15_001422"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBD6360265.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="KKO15_001423"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="KKO15_001423"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBD6360266.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="KKO15_001424"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="KKO15_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBD6360267.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="KKO15_001425"
     CDS             complement(5179..5421)
                     /locus_tag="KKO15_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBD6360268.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="KKO15_001426"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="KKO15_001426"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBD6360269.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="KKO15_001427"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="KKO15_001427"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBD6360270.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="KKO15_001428"
     CDS             complement(8338..9312)
                     /locus_tag="KKO15_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6360271.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="KKO15_001429"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="KKO15_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBD6360272.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="KKO15_001430"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="KKO15_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBD6360273.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="KKO15_001431"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="KKO15_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBD6360274.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="KKO15_001432"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="KKO15_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBD6360275.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="KKO15_001433"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="KKO15_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBD6360276.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="KKO15_001434"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="KKO15_001434"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBD6360277.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="KKO15_001435"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="KKO15_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBD6360278.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="KKO15_001436"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="KKO15_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBD6360279.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="KKO15_001437"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="KKO15_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBD6360280.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="KKO15_001438"
     CDS             23179..23382
                     /locus_tag="KKO15_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBD6360281.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="KKO15_001439"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="KKO15_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBD6360282.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="KKO15_001440"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="KKO15_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBD6360283.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="KKO15_001441"
     CDS             26387..26659
                     /locus_tag="KKO15_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBD6360284.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="KKO15_001442"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="KKO15_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBD6360285.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="KKO15_001443"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="KKO15_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBD6360286.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="KKO15_001444"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="KKO15_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD6360287.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="KKO15_001445"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="KKO15_001445"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBD6360288.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="KKO15_001446"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="KKO15_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="KKO15_001447"
     CDS             complement(31906..32304)
                     /locus_tag="KKO15_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBD6360289.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="KKO15_001448"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="KKO15_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBD6360290.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="KKO15_001449"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="KKO15_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBD6360291.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="KKO15_001450"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="KKO15_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBD6360292.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="KKO15_001451"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="KKO15_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBD6360293.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="KKO15_001452"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="KKO15_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBD6360294.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="KKO15_001453"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="KKO15_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD6360295.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9113 a         9419 c         9899 g         9196 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgccagcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gacttcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DAECOD010000027        36671 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.027, whole genome shotgun sequence.
ACCESSION   DAECOD010000027 DAECOD010000000
VERSION     DAECOD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.027"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            70..426
                     /locus_tag="KKO15_001454"
     CDS             70..426
                     /locus_tag="KKO15_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBD6360296.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            430..1557
                     /gene="dapE"
                     /locus_tag="KKO15_001455"
     CDS             430..1557
                     /gene="dapE"
                     /locus_tag="KKO15_001455"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBD6360297.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1585..1785
                     /locus_tag="KKO15_001456"
     CDS             1585..1785
                     /locus_tag="KKO15_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBD6360298.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1895..2593)
                     /gene="ypfH"
                     /locus_tag="KKO15_001457"
     CDS             complement(1895..2593)
                     /gene="ypfH"
                     /locus_tag="KKO15_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6360299.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2667..4682)
                     /gene="tmcA"
                     /locus_tag="KKO15_001458"
     CDS             complement(2667..4682)
                     /gene="tmcA"
                     /locus_tag="KKO15_001458"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBD6360300.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4697..5560)
                     /locus_tag="KKO15_001459"
     CDS             complement(4697..5560)
                     /locus_tag="KKO15_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBD6360301.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5728..6441)
                     /gene="purC"
                     /locus_tag="KKO15_001460"
     CDS             complement(5728..6441)
                     /gene="purC"
                     /locus_tag="KKO15_001460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBD6360302.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6654..7688)
                     /gene="bamC"
                     /locus_tag="KKO15_001461"
     CDS             complement(6654..7688)
                     /gene="bamC"
                     /locus_tag="KKO15_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBD6360303.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7705..8583)
                     /gene="dapA"
                     /locus_tag="KKO15_001462"
     CDS             complement(7705..8583)
                     /gene="dapA"
                     /locus_tag="KKO15_001462"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBD6360304.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8729..9301
                     /locus_tag="KKO15_001463"
     CDS             8729..9301
                     /locus_tag="KKO15_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBD6360305.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9301..9771
                     /gene="bcp"
                     /locus_tag="KKO15_001464"
     CDS             9301..9771
                     /gene="bcp"
                     /locus_tag="KKO15_001464"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBD6360306.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10024..10641
                     /gene="hyfA"
                     /locus_tag="KKO15_001465"
     CDS             10024..10641
                     /gene="hyfA"
                     /locus_tag="KKO15_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBD6360307.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10641..12658
                     /gene="hyfB"
                     /locus_tag="KKO15_001466"
                     /pseudo
     CDS             10641..12658
                     /gene="hyfB"
                     /locus_tag="KKO15_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12669..13616
                     /gene="hyfC"
                     /locus_tag="KKO15_001467"
     CDS             12669..13616
                     /gene="hyfC"
                     /locus_tag="KKO15_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBD6360308.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13633..15072
                     /gene="hyfD"
                     /locus_tag="KKO15_001468"
     CDS             13633..15072
                     /gene="hyfD"
                     /locus_tag="KKO15_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBD6360309.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15084..15734
                     /gene="hyfE"
                     /locus_tag="KKO15_001469"
     CDS             15084..15734
                     /gene="hyfE"
                     /locus_tag="KKO15_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBD6360310.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15739..17325
                     /gene="hyfF"
                     /locus_tag="KKO15_001470"
     CDS             15739..17325
                     /gene="hyfF"
                     /locus_tag="KKO15_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBD6360311.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAISWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            17315..19029
                     /gene="hyfG"
                     /locus_tag="KKO15_001471"
                     /pseudo
     CDS             17315..19029
                     /gene="hyfG"
                     /locus_tag="KKO15_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19039..19584
                     /gene="hyfH"
                     /locus_tag="KKO15_001472"
     CDS             19039..19584
                     /gene="hyfH"
                     /locus_tag="KKO15_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBD6360312.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19581..20339
                     /gene="hyfI"
                     /locus_tag="KKO15_001473"
     CDS             19581..20339
                     /gene="hyfI"
                     /locus_tag="KKO15_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBD6360313.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDLRLNSIVQQLFRVLRGLHD"
     gene            20332..20745
                     /gene="hyfJ"
                     /locus_tag="KKO15_001474"
     CDS             20332..20745
                     /gene="hyfJ"
                     /locus_tag="KKO15_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBD6360314.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20775..22787
                     /gene="hyfR"
                     /locus_tag="KKO15_001475"
     CDS             20775..22787
                     /gene="hyfR"
                     /locus_tag="KKO15_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBD6360315.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SKQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            22809..23657
                     /locus_tag="KKO15_001476"
                     /pseudo
     CDS             22809..23657
                     /locus_tag="KKO15_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(23695..24756)
                     /locus_tag="KKO15_001477"
     CDS             complement(23695..24756)
                     /locus_tag="KKO15_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD6360316.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            24969..26432
                     /gene="bepA"
                     /locus_tag="KKO15_001478"
     CDS             24969..26432
                     /gene="bepA"
                     /locus_tag="KKO15_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBD6360317.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            26453..26812
                     /gene="arsC"
                     /locus_tag="KKO15_001479"
     CDS             26453..26812
                     /gene="arsC"
                     /locus_tag="KKO15_001479"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBD6360318.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26945..27691)
                     /gene="hda"
                     /locus_tag="KKO15_001480"
     CDS             complement(26945..27691)
                     /gene="hda"
                     /locus_tag="KKO15_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBD6360319.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(27741..29030)
                     /gene="uraA"
                     /locus_tag="KKO15_001481"
     CDS             complement(27741..29030)
                     /gene="uraA"
                     /locus_tag="KKO15_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBD6360320.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29116..29742)
                     /gene="upp"
                     /locus_tag="KKO15_001482"
     CDS             complement(29116..29742)
                     /gene="upp"
                     /locus_tag="KKO15_001482"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBD6360321.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30067..31104
                     /gene="purM"
                     /locus_tag="KKO15_001483"
     CDS             30067..31104
                     /gene="purM"
                     /locus_tag="KKO15_001483"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBD6360322.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31104..31742
                     /gene="purN"
                     /locus_tag="KKO15_001484"
     CDS             31104..31742
                     /gene="purN"
                     /locus_tag="KKO15_001484"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBD6360323.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31914..33980
                     /gene="ppk1"
                     /locus_tag="KKO15_001485"
     CDS             31914..33980
                     /gene="ppk1"
                     /locus_tag="KKO15_001485"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBD6360324.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            33985..35526
                     /gene="ppx"
                     /locus_tag="KKO15_001486"
     CDS             33985..35526
                     /gene="ppx"
                     /locus_tag="KKO15_001486"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBD6360325.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35565..>36671)
                     /gene="pdeF"
                     /locus_tag="KKO15_001487"
     CDS             complement(35565..>36671)
                     /gene="pdeF"
                     /locus_tag="KKO15_001487"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD6360326.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8493 a         9698 c         9538 g         8942 t
ORIGIN      
        1 tcatttgcag gcaaaatgtt ttcaccctta aatgagtatt tattctcata aatcgaaaaa
       61 ggattcatta tggttacact ttacggcatc aaaaattgtg acaccattaa aaaggctcgc
      121 cgttggctgg aagccaataa catcgactat cgttttcatg attaccgcgt cgatgggctg
      181 gacagcgaat tattgaacgg ttttatcaac gaattaggct gggaagcgtt actcaacacc
      241 cgtggtacaa cctggcgtaa actggacgaa actacccgca ataaaatcac cgatgcggcc
      301 tctgcggcgg cattaatgac tgaaatgcct gcaattatca aacgtccatt gctctgcgcg
      361 cctggtaagc ctatgctgct gggtttcagt gattccagtt atcagcaatt tttccatgag
      421 gtgtagtcta tgtcgtgccc ggttattgag ctgacacaac agcttattcg ccgcccttcc
      481 ctgagtcctg atgacgcagg atgtcaggct ttgttgattg aacgtttgca ggcgatcggt
      541 tttaccgttg aacgcatgga ttttgccgat acgcagaatt tttgggcatg gcgtgggcaa
      601 ggtgaaacgt tagcctttgc cgggcatacc gacgtggtgc cgcctggcga cgccgatcgt
      661 tggatcaatc cgccgtttga acccaccatt cgtgacggca tgttattcgg gcgcggtgcg
      721 gcagatatga aaggctcgct ggcggcgatg gtggtagcag cagaacgttt tgtcgcacaa
      781 catcccaacc atacggggcg actggcattt ctgatcactt ctgatgaaga agccagtgcc
      841 cacaacggta cggtaaaagt cgtcgaggcg ttaatggcac gtaatgagcg tctcgattac
      901 tgcctggtcg gcgaaccgtc gagtatcgaa gtggtaggtg atgtggtgaa aaatggtcgt
      961 cgcggatcgt taacctgcaa cctcaccatt catggcgttc aggggcatgt tgcctaccca
     1021 catctggctg acaatccggt acatcgcgca gcacctttcc ttaatgaatt agtggctatt
     1081 gagtgggatc agggcaatga attcttcccg gcgaccagta tgcagattgc caatattcag
     1141 gcgggaacgg gcagtaacaa tgttattccg ggtgaactgt ttgtgcagtt taacttccgc
     1201 ttcagcaccg aactgactga tgagatgatc aaagcgcagg tacttgccct gcttgaaaaa
     1261 catcaactgc gctatacggt ggattggtgg ctttccgggc agccattttt gaccgcgcgc
     1321 ggtaaactgg tggatgcggt cgttaacgcg gttgagcact ataatgaaat taaaccgcag
     1381 ctactgacca caggcggaac gtccgacggg cgctttattg ctcgcatggg ggcgcaggtg
     1441 gtggaactcg ggccggtcaa tgccactatt cataaaatta atgaatgtgt gaacgctgcc
     1501 gacctgcagc tacttgcccg tatgtatcaa cgtatcatgg aacagctcgt cgcgtgatga
     1561 gtggttctgc aagaggaaat aagcatggac tggctggcta aatattggtg gattctggtg
     1621 attgtctttt tggtaggcgt cctgctgaac gtgattaaag atctcaagcg cgtcgaccat
     1681 aagaaatttc tcgccaacaa gccggagctt cccccgcatc gtgatttcaa cgataagtgg
     1741 gacgatgacg acgactggcc gaaaaaggat caaccgaaga agtaagctga gtgctcaacc
     1801 gtaggccgga taaggcgtta acgccgcatc cggcaatggt gaacgatccc tgatgcgacg
     1861 ctcacgcgtc ttatcaggcc aggtaatctg tcttttacat catttcaatc acatcatcat
     1921 cgccaggctt accgccactt agcgcttggt cgaaataatg tttcggaatg gtataacgca
     1981 agtgatccag ggcaaattgc atactgcgat tgtcgatcgc atggcctaaa tcctccacga
     2041 tatccagcgt cacgtcacca ccggcactga ttaacgcttc ctgcgccgca actgcatgcg
     2101 ccagatcgat aaccggatct tcaccaccat gaatcaagtg aattgtcgtg gctgtagacg
     2161 cggtctccgg caggctcgca tagcgtccgt taaaggcaat gacgcgcgaa gcaaggcccg
     2221 gttcggcttt aatgctctcc agcgccataa tcgcgccttg tgagaaacca attagcgccg
     2281 tggcgttagc ccctactccg ctctgtttct gccagtagcg taccgtttca ataaacgtcg
     2341 gcatgatggc atccacacgc gcctggcgat tatcttccgt aataccctgc actgaaaacc
     2401 actgacgccc cgccggatta ccgctcggct ccgcgccacc gacactcacc accagcgcat
     2461 caggaaacag cggtgcaaac cagctgccta tttcccccat tgccaccggg ttatccccga
     2521 caccgtggaa aagcagtaac aactgttgtg caggtttatc cgggctttga acaacaaaat
     2581 ggtcatgttt catggcgaac tccttaactg atgtcattaa ttttacgccg ctgactcaca
     2641 atgaccatgg gagaaaattg aaggagtcag tgaaaaaatt gccattgcgt tatgcgatcg
     2701 cgcagacgct cagtgcgaac ctcatttagt gcgaacagcg cctgcgcggc ctcttcccgc
     2761 tggcgaacca gtaacatctt gcggcctgaa agtttaagtg tggtacataa ctgcgcgtca
     2821 ctggcgtttt tctgtaaacg cccacgcagc gccggtaatg ccagttcact ggtttgtagc
     2881 agacgcaata agcaacctaa cgatgttaat agcggacgat gagcgaaagc aaaaccggcc
     2941 agttcaagcc agtcgtcgtc agaaaggatg gcatcgttta gtggatcaac aggaagcatt
     3001 tcgccattcc actgcgcgag agcttgcgca tcgcgacgta aacggtaatg ctcacgttca
     3061 gccagctgtt tacccgcatc actcatcggt aacagcgcca tcgccgtata gcaaccgctg
     3121 ctggcttccc gatgattacc catccgcacc agcacaaaac cgcagcgttg ccagaaacgc
     3181 cataactccc cagtgtaacc aaaactcacc gaaagatagt cgaggtcatg cgtatattgc
     3241 aaagctccag caataagttg tcgccctgtg ccttcccgct gacgagccgg atgaactgct
     3301 atccggctaa cccgccttcc gcgcaatgtc gccgccagtg gattgctgcc gtgcgccgcc
     3361 agcgactggg ccaccagatt accccgcggg cgacgaaaac ctgcccatac cgcctgactg
     3421 agttcttgag ataatccgcc ctcatccacc agccacaacg ccccggcaat ctcgttttcg
     3481 ccagccgcct gtaaaaaatg ttgccctggt gcatccatca tccggcgtaa atccagcggc
     3541 gaggtccggt agtgcgcacc agacaataac tgataaacct ttaacggcgt ttctggctcg
     3601 cttcgccata acgtttgttc aaatgcggaa atgacgatat tgccttgtgg tgtatgggtg
     3661 aagttttcat cgttaaaaac cagtgcctca ctaaccattt tttccagcgg gcatccctgt
     3721 gcccagcgga tcggctgttg cagttcaaaa cggtgtaaat gcggaaagcg agcgcaaaat
     3781 ttcagcaaaa aaccacgtcc ggtaccttcg tagccctgca ccgtagtggt taacaacgtt
     3841 cgaggaaaac gcgataccag ttgatgcaac aacggcgcag gtatggctgc ggcttcgtcg
     3901 accaccagcc agtcggcttg ctcatcgctg gctaacaagg catccggtgc aataaagcga
     3961 aacttctcgc ccgcaaattg tgccagtaca tccgttgccg cttttgcggg cgcggtgaca
     4021 atcgcactgc ccgcaatacg agaaatgagt tgccctgcca acgccgactt accgcgccca
     4081 cgcgcagccg ttaccgctgc cacgcccggc ggcatggtca gtagctgctg taagagttgc
     4141 tgttgttctg gttgtggtgc gccggtcgcg gggtgccagt cagtacgggg agtaaaatgc
     4201 gccaacgaga acggctggtt ttgccgccag aggatagcgt cgttatccgc cgtaagtacg
     4261 cgtttgagat gctggacaaa atgcggcgtc gcaatagggt cagggcaatc actccagcgc
     4321 agcgagtcgg catcaggttg gttttcccac tcttcccata cagggagtaa caaaaccagc
     4381 cagcttcccg ctttcaacgt tccgctaagt gctgcaaagg cagcggcatc aaagccgtgg
     4441 cgggcgtcga ataccgcatg ccggaactcg cgcccaagta aagtttgtag cgccgagggt
     4501 gaacagtggt tttcagcatc tagctgcggc gaaatccaca gccagtcgcc aggtaaggca
     4561 tcacgcaact taagagcatg atcaaaacac caaccctctt ccccgctcaa caccagcaag
     4621 cggcagatcc cttcacgttt catttgcgct gttaatgtgt aaagcgcagt cagttcagcc
     4681 atccctgccc cggaaattaa atgcttttac caaaagtatt gcattgtgcc ggatcgccgc
     4741 tgtcgaaacc acgtttaaac cagctgtagc gttgctgaga agtgccatgt gtgaaactgt
     4801 ctggtactac tcgcccctga ctttgctgtt gtaaacggtc atcgccgatg gcctgcgccg
     4861 cattcagcgc ctcttccaga tcgccagttt ccagaacgcc ttgctgctgc atactatgcc
     4921 cccagacacc ggcaaaacag tcggcctgga gttccatacg cacagataag cggtttactt
     4981 ccgcctgcgt cgcgttttgt tgcagttgac gaactttcgg ctcgatgcct aacagtttct
     5041 gcacatgatg accgacttca tgggcgataa cgtacccctg ggcaaaatcg ccatccgcgc
     5101 caagtttgtc tttcatgtca tcatagaagg agagatcgat ataaactgtg ccatccgccg
     5161 ggcaatagaa cggccccata atggactggc ccgccccgca gccggtgcgc gtcattccac
     5221 ggtacatgac cagcttcggt tgctgatagg tcttacccat cttttcgaac tgttgtcccc
     5281 aggtgtcttc cgtggttgcc agaatcaccg aggtgaattt tgcggcttcg tcttcattag
     5341 ggctaattga ccgcgttgat tgttgttgag aaaccggctg cccggtcatc aacccggtta
     5401 aatcaacgcc atagtagcct gcaaccagca cgactatcag taaaatcagc ccgcctttac
     5461 cgctcggcag acggaaaccg ggaccgccca ttgacggacc gccagagctg ttgcgcctgt
     5521 cttcaacatt gtcactttca cgtcgccctt gccaacgcat agatacctca acaatatatt
     5581 cattatagaa atgatcgtag gtggttcaga ggaagattac cacaggaaag cgggatgaaa
     5641 tggcggagga atgagcgttc aatgcatata ttccgagagc ggttgtcttg ccgtgccagc
     5701 tgcacggcaa gatgatgaac agaaaaatca gtccagctgt acacccaggc ggcgggcgac
     5761 ggcttcatag gcttcgatca ggccaccgag gctctggcgg aaacggtctt tgtccatttt
     5821 ctccagtgtt tctttgtccc acaggcggct gccgtccggg gagaactcat cgcccagtac
     5881 cacttcacct ttgtacagac caaactccag cttgaagtcg accagaatca gaccagcatc
     5941 atcgaacagt tttttcagca cgtcgttcgc tttgtaggtc agctctttca tacgcgccag
     6001 gttctctttg ctcacccagc caaaggtttc gcagtaagat tcgttgacca tcggatcgtg
     6061 catggcatcg tttttcagga acagatcgaa cagcggcggg ttcagctcaa taccttcttc
     6121 gattccaaga cgtttcacca gagagccagc agcacggtta cgcacgacac actcaaccgg
     6181 caccatatcc agctttttca ccagacattc ggtatcggag agcagacgct ccatttgagt
     6241 cgggataccc gcttcagcca gtttgctcat aatgaagtag ttgaacttgt tgttcaccat
     6301 acctttgcga tcaaactgct caatgcgcgc gccatcccct gctgacgtat cattgcggaa
     6361 ttcgagcacc aacaggtccg ggttttccgt gctgtatacg gttttcgctt taccacgata
     6421 caactcagct tgcttttgca tctttatcac tcctgggtgt gaattaacgt tttaaaatct
     6481 tttgctgtct ggtgtgccgg atgttttgtc ggatgcggcg tgaacgcctt atccgaacta
     6541 cgtccgacaa acaaatttcg tgcgaattac cgctaaaatc gcgttttcct gccgacgcac
     6601 acgtttgcgt atcatatcag aaaaaagggc cggatgattc cagccctgta tttttacttg
     6661 ctaaacgcag cctggaagac agctaccagc gcgtcgttct gactctgagt cagagtatga
     6721 cctttcggat cgatgaactg taggctgctg cggttatcta aatcgccaac ctgcagttta
     6781 tagtcaccgg atgccaggcc tggatcgctc gcgcccagtt cctgccagtc gctgtcagac
     6841 agcggcttat aagttacggc catgttgccc tgcgaacggg tgctgtcggt cactttcatg
     6901 cccacttttt ccagcgccgc tggcagacgt tgccaaacca cattgaacgg cccgcgtacg
     6961 accagcattg gtaaaccggt gtcatcagct gcactttgta cgtccatagt ggtggaggca
     7021 cgattttgcg cagcgttcgc ggcgtcagtg gcagatttat ccagaccggc ggaaataacg
     7081 ttcatcatct ccgtgctgta acgctgcatg gaagccgcgt ctgcaaccgg tttgcccgcc
     7141 tgttccaggt tcagcagttt aaccgtaacc gcctgctgat aaccctgcgg cttaacagag
     7201 atttgataac gaccacgata ctgctcgtct tcgtccagac ggttccattg tacccaatcg
     7261 gtggtcagtg tctgaccagc atcatcacgt tgggtgatgg tgtagttttt cgcctgcagc
     7321 acgctaacca cctgcggcca cagagtattg ccacgaccat tttccaccag caatgaagcg
     7381 gtatcgcccg tgaactgggt acgcgcgcca gaaaccagtg ccaacggctg ggctggtgga
     7441 cgaatgtcca gcgccttacc gacagcacca ctaccgttgg tcaccgggat tgcataatca
     7501 ccggaggtca ccggcaaaat cattccagcc ggggcatgaa gctccgcaag cggtgccgct
     7561 tccaggtagg cttcatcacc actgacctga cgcttatagc gtgagtcaga actacaggca
     7621 gcgagtaata aaacaagcga aacacccgca acctttgcca ggcgcgactt ttgaacagag
     7681 taagccatca aatctcccta aactttacag caaaccggca tgcttaagcg ccgctctgac
     7741 cgtctcacga ccactgtcgg tgattggtgt cattggcagg cgcagcgtat cggtcgccac
     7801 aagacccagt tccttacatg cccatttcac cgggattgga ttgggttcga caaatagttt
     7861 gttgtgtaat ggcatcagac gctgattaat aacgcgtgcc tcggcaaaat gcccttctgc
     7921 tgccagtttg cacatctggg ccatatcacg cgctgcgacg ttagccgtaa cggaaataac
     7981 tccatgaccg cccaattgca tgaagtccag cgcgctcgca tcatcgccgc tcagcagaac
     8041 aaaatcatct gaaaccagct ctttgatctg gtttacacgc gttaagttcc ctgttgcctc
     8101 tttgattccg ataatatttt ttactttcgc cagacggccc accgtttccg ggagcagatc
     8161 gcagccagta cgggacggca cattatacag aatttgcggc aggtcagtat gctcagcgat
     8221 ggctttgaaa tgctgataca aaccttcttg cgacggacga ttgtagtaag gggttaccgt
     8281 caggcagccg acgataccac tgtcattgaa gcgctgcgtc aggctaatgg cttccgcagt
     8341 agcgttagcg ccggtcccgg caattaccgg aatgcgccca tcagccagat ccagcgtcat
     8401 catcaccaca tcagcatgtt cgtcatgatt taaggtagcg gactcgccag tggtgccaac
     8461 agaaacgatc gccgaagtac cgctggcgac atgataatca atcagttttt tcaagctagc
     8521 ccgacagaca ttaccttttt catccatcgg agtaacaatc gcgacaatac ttcccgtgaa
     8581 catgggccat cctctgtgca aacaagtgtc tcaatggtac gtttggtatg gcattaaaag
     8641 caagcagaca gaaccgttct gattgttgta tgcatgattt ttttatgctt tccttaagaa
     8701 caactcaccc cttaaaggaa taaccagttt gacactgtca tcgcaacatt atctggtgat
     8761 cactgcgttg ggtgccgatc gccctggaat tgtgaacacc atcacccgtc atgtcagtag
     8821 ttgcggctgt aatattgaag acagtcgcct ggcgatgctg ggagaagagt tcacgtttat
     8881 catgctgctt tccggttcat ggaatgccat cactctgatt gaatcaacgt taccgttgaa
     8941 aggtgccgaa ctggatcttt taatcgtgat gaagcgcacg acggcgcgtc cgcgtccgcc
     9001 aatgccagca tctgtctggg ttcaggtcga tgttgcagac tccccgcatt taattgaacg
     9061 cttcacagca cttttcgacg cgcatcatat gaacattgcg gagctggtgt cgcgcacaca
     9121 acctgctgaa aatgaacggg ctgcgcagtt gcatattcag ataaccgccc acagccccgc
     9181 atctgcggac gcagcaaata ttgagcaagc gttcaaagcc ctatgtacag aactcaatgc
     9241 acaaggcagt attaacgtcg tcaattattc ccaacatgat gaacaggatg gagttaagta
     9301 atgaatccac tgaaagccgg tgatatcgca ccgaaattta gcttgccgga tcaagacgga
     9361 gaacaagtta atttgaccga cttccaggga cagcgtgttc tggtttattt ctacccgaaa
     9421 gccatgaccc ccggctgtac cgtacaggcc tgcggcttac gcgataacat ggatgagttg
     9481 aaaaaagcgg gcgttgatgt gctgggtatc agcaccgata aacccgaaaa actctcccgt
     9541 tttgcggaaa aagagctgct taactttacg ctcctgtctg atgaggacca ccaggtgtgc
     9601 gaacaattcg gcgtctgggg tgaaaagtcc ttcatgggca aaacctacga tggtattcat
     9661 cgcatcagct tcctgattga cgctgatggc aaaatcgaac atgtctttga cgatttcaaa
     9721 accagcaatc accacgacgt tgtgctgaac tggctgaaag aacacgcctg attactttgc
     9781 tccattccgt gctggctgcg cttgcggcca gcatacctca cttctcgtga tcaagatcac
     9841 attctcgctt tcccctgcga cacgggtgtc gaatccattt tttgctgaac gttaatgacc
     9901 atcatttttg taccgttcag aatccagtta atacataact tattgaatag attgagttaa
     9961 tcagaatggc atcctttata caatatgaaa tgcaatgttt catatcattt tcaaggagcc
    10021 gacatgaacc gctttgtggt ggccgaacca ctgtggtgta caggatgtaa tacctgtctc
    10081 gctgcctgtt cggacgtgca taaaacgcaa ggtttacagc aacacccgcg cctggccctg
    10141 gcgaagacgt caacaatcac tgcccctgtc gtgtgtcatc actgtgagga agccccttgc
    10201 ctgcaggtct gcccggtcaa tgccatctct cagagggatg atgcgatcca actcaacgaa
    10261 agcctctgta ttggctgcaa gctttgcgcc gtggtctgcc catttggcgc aatcagcgct
    10321 tcaggaagcc gtccggtgaa tgcccatgcg caatatgttt ttcaggctga aggctcactc
    10381 aaagacggcg aagaaaacgt gccgccccaa catgcgttgc tgcgctggga accgggtgtc
    10441 cagaccgtcg cggtgaaatg cgacctgtgt gatttcttgc cagaaggtcc ggcctgcgtt
    10501 cgcgcttgcc cgaatcaggc gttacggctg atcaccgacg atagcctgca acgtcagatg
    10561 aaagaaaaac agcgccttgc cgcaagctgg tttgccaatg gcggggagtc tcccctttcc
    10621 ctcactcagg agcaacacta atggatgccc tgcaattatt aacctggtcg ctgatcctct
    10681 atctgtttgc cagtctggct tcgctgtttt tactcggtct ggacagactg gctattaagc
    10741 tttccggcat cacatcgctg gtgggcggcg tgattggcat catcagcgga attacgcaat
    10801 tacatgctgg tgtaacttta gtcgcccatt ttgccacccc ttttgacttt gccgatttaa
    10861 ccctgcgaat ggatagcctc tcggcattta tggtgctagt tatctccttg ctggtggtgg
    10921 tttgttcgct ctattcattg acttatatgc gcgaatacga gggcaaaggc gcggcggcga
    10981 tgggcttctt tatgaatctt ttcatcgcat cgatggttgc cctgctggtg atggacaacg
    11041 ctttttggtt catcgtgctg tttgaaatga tgtcgctgtc ttcctggttt ctggtcattg
    11101 ccaggcagga taaaacgtcg atcaacgctg gcatgctcta cttttttatc gcccacgccg
    11161 gatcggtgct gattatgatc gccttcttgc tgatggggcg cgaaagcggc agcctcgatt
    11221 ttgccagttt cgcacgcttt cactttctcc ggggctggcg tcggcggtgt ttctgctggc
    11281 ctttttcggt tttggcgcga aagccgggat gatgccgttg cacagctggt tgccgcgcgc
    11341 tcaccctgcc gcaccatcgc acgcttcggc gttgatgtct ggcgtaatgg tcaaaatcgg
    11401 tattttcggc atcctgaaag tagcgatgga tctgctggcg caaacgggtt tgcctctgtg
    11461 gtggggcatt ctggtgatgg cgatcggcgc aatctccgcg ctcctgggcg tgctatatgc
    11521 gctggcggaa caggatatca aacggctgct ggcctggagt accgtcgaaa acgtcggcat
    11581 tattttgctg gcagtcggtg tggcgatgga cggtctgtca ctgcacgacc cgctgctcac
    11641 cgtggttgga ctgctcggcg cactgtttca tctgctcaac catgcgctgt tcaaagggct
    11701 gctatttctc ggcgcggacg cgattatttc gcgtttgcat acccacgaca tggaaaaaat
    11761 gggggcactg gcgaaacgga tgccgtggac agccgcagca tgcctgattg gttgcctcgc
    11821 gatatcagcc attcctccgc tgaatggttt tatcagcgaa tggtacacct ggcagtcgct
    11881 gttctcacta agtcgtgtgg aagccgtagc gctacaactt gcgggtccta ttgctatggt
    11941 aatgctggca gtcactggtg ggctggcagt aatgtgcttc gtaaaaatgt acggtattac
    12001 tttctgtggt gcgccgcgca gtacacacgc tgaagaggca caggaagtgc caaatacgat
    12061 gatcgtcgcc atgctactgc tcgcggcact ctgcgtatta attgcgctta gtgccagttg
    12121 gctggcaccg aagataatgc atattgccca tgcgtttacc aatacccctc ccgtcactgt
    12181 cgccagcgga atagcacttg tacccggcac gtttcataca cgggtcactc cttcattact
    12241 gttgctgtta ctactggcga tgcctttgct gccaggcctt tactggctgt ggtgtcgttc
    12301 gcgccgcgca gcgtttcgtc gcacaggaga tgcctgggca tgcggctacg gctgggaaaa
    12361 cgcgatggct ccgtcaggca atggcgtgat gcagccgctg cgtgtggtct tctctgcgtt
    12421 atttcgtcta cgacaacagc tcgaccctac gctgaggcta aataaaggtc ttgcgcacgt
    12481 caccgccagg gctcagagca cagaaccctt ctgggatgag cgggtgatcc gccccatcgt
    12541 gagcgccacc caacggctgg tcaaagaaat acagcatctg caaagcggcg actttcgtct
    12601 ctattgcctg tatgtggtcg ccgcactggt tgtgctgcta atcgctattg ccgtctaagg
    12661 aaatcaccat gagacaaact ctttgcgacg gatatctggt catttttgcg ttagcacagg
    12721 ccgtgattct gctgatgcta accccacttt ttacgggtat ttcccggcag atacgcgcgc
    12781 gtatgcactc ccgccgcggg ccggggatct ggcaggatta tcgcgatatc cacaaactgt
    12841 ttaaacgcca ggaagttgcg ccgacatctt caggtctgat gttccgcctg atgccgtggg
    12901 tattaatcag cagcatgctg gtgctggcga tggccttacc actatttatt accgtttccc
    12961 cttttgcggg cggcggcgat ctgatcaccc ttatctatct tcttgccctg tttcgttttt
    13021 tctttgctct ttccgggctg gataccggaa gtccgtttgc gggagtcggt gccagtcgcg
    13081 agttgacgct cggcattctg gtcgaaccaa tgcttattct ctcactgctg gtattggcgc
    13141 tgatagcagg ttccacgcat atcgagatga tcagcaatac gctggctatg ggctggaact
    13201 cgccgctaac caccgtactg gcgttactgg cctgtggttt tgcctgcttc attgagatgg
    13261 gaaaaattcc ctttgatgtt gctgaagcag aacaggaatt acaggaaggc ccgctgaccg
    13321 aatattccgg tgccgggctg gcgctagcga aatgggggct ggggctgaaa caggtcgtga
    13381 tggcagcact gtttgtggcc ctgtttctgc cctttgggcg cgcgcaagaa ctttctctcg
    13441 cctgcctgct gacttcactt gtcgttacgc tgctcaaggt tttgctgatt tttgtactgg
    13501 cctcaatcgc agaaaacacg ctggcacgcg ggcgtttttt actcattcac catgtgacct
    13561 ggcttggctt cagccttgct gcgcttgcat gggtcttttg gttaaccggt ctgtaaggag
    13621 cactgacgga atatggaaaa tcttgctctg acgacgttaa tgctgccttt tatcggcgca
    13681 ctggtcgttt cgttttcgcc acaacgtcgg gccgccgaat ggggggtttt gttcgccgcg
    13741 ctgaccacgc tgtgcatgtt gtcgctgatc tccgcgtttt atcaggccga taaagttgcc
    13801 gtcacgttga cgttggtcaa cgtgggggat gtggcattgt ttggcctggt cattgatcgc
    13861 gtgagtacgc tgattctgtt tgtggtggtg tttctcggtt tgctggtcac gatctactcc
    13921 acgggttatc tgacggataa aaatcgcgaa cacccgcata acggcacgaa tcgttattac
    13981 gcatttttgc tggtgtttat cggcgcgatg gcgggactgg tagtctcctc gacgctgctc
    14041 ggtcagttgt tgttttttga aattacgggc ggctgctcct gggcgttgat cagttattac
    14101 cagagcgata aagcgcagcg ttcagcacta aaagcgttgc ttatcaccca tatcggttcg
    14161 ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt tgtgcttagc
    14221 gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat cctgtttgcc
    14281 gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc aatggaagcg
    14341 ccaacaccga tcagcgccta tctccacgcc gcatcgatgg tgaaagtggg cgtttacatt
    14401 tttgcccgcg ccattatcga cggcggcaat atcccgcatg tgattggcgg cgttggcatg
    14461 gtcatgacac tggtcaccat tctttatggc tttctgatgt atttgccaca gcaggatatg
    14521 aagcggttgc tggcctggtc gaccatcact caacttggct ggatgttctt cggcttgtcg
    14581 ctctccatct tcggctcgcg gctggcgctg gagggtagca tcgcctacat cgtcaaccac
    14641 gcgttcgcta aaagcctgtt tttccttgta gcaggtgcgc tgagttacag ctgcggcacg
    14701 cgcttgttgc cgcgtctgcg tggcgtattg cacaccctgc cgttgccagg cgtgggtttc
    14761 tgcgtggcag cgctggcaat taccggtgtg ccgccgttca acggcttctt cagtaaattc
    14821 ccgctgtttg ccgccggttt tgcgttgtca gtggagtact ggatcctgct gcccgccatg
    14881 attctgctga tgattgaatc ggtcgccagt ttcgcctggt ttattcgctg gtttggtcgc
    14941 gtcgtgccgg gcaaaccgag cgaggccgtc gccgatgccg caccgctgcc agggtcaatg
    15001 cgcctggtgt tgattgtact gattgtgatg tcgctgattt ccagcgtaat cgccgcgacc
    15061 tggttgcagt aaggagatga tgaatgaccg gttctatgat cgtaaataat ctggcgggac
    15121 tgatgatgct gacatcgctg tttgtgatta gcgtcaaaag ctatcgcctg tcatgcggat
    15181 tttacgcctg ccagtcgctg gtgctggtgt ctattttcgc cactctctcg tgcctgttcg
    15241 ccgcagagca actgctgatc tggtccgcca gcgcctttat caccaaagtg ctgctggtac
    15301 cgttaatcat gacttacgct gcacgaaata ttccccagaa catcccggaa aaagcgttat
    15361 tcggtccggc aatgatggca ctgctcgcgg cgttaattgt cctgctttgc gcatttgtcg
    15421 ttcagcccgt gaagctaccg atggctaccg ggctgaaacc ggcgctggcg gtagcgttag
    15481 gtcattttct gcttggcctg ctgtgcattg tcagccaacg caatatcctg cggcaaattt
    15541 ttggttactg cctgatggaa aacggctccc atctggtgct ggcgcttctt gcctggcgag
    15601 caccggaact ggtggaaata ggtatcgcta ccgacgccat cttcgccgtc attgtgatgg
    15661 tgttactggc aagaaaaata tggcgtaccc acggcacgct ggacgtgaac aacttgaccg
    15721 cgctgaaggg ataatgagat gagttattct gtgatgttcg ctttactcct gctcacgccg
    15781 ctgctttttt cgctgctctg ttttgcctgc cggaaacggg ggctctctgc gacttgtacg
    15841 gtgactgtat tacatagctt agggatcaca ctgctgctga ttctggcact ctgggtggtc
    15901 caaactgccg ctgatgcagg agaaatattc gctgcgggac tgtggcttca tattgatggt
    15961 ctgggcggtt tgttcctcgc cattcttggt gtgattggct ttctcaccgg tgtttactcg
    16021 attggctaca tgcgtcatga agtggcacac ggcgagcttt cacccgttac actgtgcgat
    16081 tactacggtt tcttccatct gtttttgttc accatgctgc tggttgttac cagcaataac
    16141 ctgattgtga tgtgggcggc gatcgaagcc accaccttaa gctcggcgtt tctggtaggc
    16201 atttacggtc agcgttcatc gctggaatca gcatggaagt acatcattat ttgtactgtt
    16261 ggtgtcgctt ttggtctgtt cggtaccgtg ctggtatacg ccaacgccgc cagcgttatg
    16321 ccgcaggcag aaatggcgat atcctggagc gaggttctta agcaatcgtc cttgcttgac
    16381 ccaacattaa tgctgttggc ctttgtgttt gtgctaattg gctttggtac caaaaccggg
    16441 ctatttccca tgcacgcctg gctgccggat gctcacagtg aagctccgag tccggtcagc
    16501 gccctgctct ccgccgtatt gctgaactgc gcgctgttgg tgctgattcg ctattacatc
    16561 attatttgcc aggccatcgg cagcgatttc cccaaccggt tgttgctcat cttcggcatg
    16621 ttgtcggttg ccgtggcggc atttttcatt ctggtacagc gggacattaa gcgtctgctg
    16681 gcgtactcca gcgtggagaa catggggctg gtcgcggtgg cgctaggcat tggcgggccg
    16741 ctgggaattt ttgccgcgct gctgcacacc ttaaaccaca gtctggcaaa aacgctgctg
    16801 ttctgcggtt ccggcaatgt actgctcaag tacggcacgc gcgatctcaa cgtcgtctgt
    16861 gggatgctca aaatcatgcc atttaccgcc gtgctgtttg gcggcggtgc gctggcgctg
    16921 gcgctggcag ggatgccgcc cttcaacatt tttcttagcg aatttatgac cgttaccgca
    16981 ggactggcac gtaatcacct gctgattatc gtcctgctgt tattgctgtt aacgctggtg
    17041 ctggcgggcc tggtacggat ggctgcgcgg gtgttaatgg cgaaaccgcc gcaggccgtt
    17101 aaccggggtg atctcggctg gttgaccacc tcgccaatgg tgattctgct ggtcatgatg
    17161 ctggcgatgg gaacgcatat tccacaacct gtcatcagga tcctggcggg cgcttccact
    17221 atagtcctct cagggacgca cgacctgcct gcacaacgta gcacctggca tgattttttg
    17281 ccttcaggca ccgcatctgt ttcggagaaa cacagtgaac gttaattcat cgtcaaatcg
    17341 tggcgaagcg attctcgccg ccctgaaaac gcagttcccc ggcgcggtgc tggatgaaga
    17401 gcgacaaacg cctgaacagg tcaccattac ggtgaaaatc aatctgctgc ctgacgttgt
    17461 acattatctt tattatcaac atgatggctg gcttccagtc ctgtttggca acgacgagcg
    17521 gacacttaac ggtcattacg cggtttatta tgccctttca atggaagggg ccgaaaaatg
    17581 ctggatcgtg gtgaaggcac tggtcgatgc cgacagtcgg gagtttccgt cagtcacacc
    17641 gcgcgtccct gccgcggtct ggggcgagcg agaaattcgc gatatgtacg ggctgattcc
    17701 ggttggcctg ccggatcagc gtcgcctggt gttgcccgat gactggccgg aagatatgca
    17761 tccgctgcgc aaagatgcga tggattatcg actgcgccct gaaccgacga cagattccga
    17821 aacgtatgcg tttatcaatg agggcaacag cgatgcgcgg gtgatccctg tcggcccgct
    17881 gcatatcacc tcctatgaac cgggtcactt ccgcttgttt gtggatggcg agcaaattgt
    17941 cgatgctgat taccgcctgt tttatgtcca tcgcggcatg gagaaactgg cagaaacgcg
    18001 gatgggctac aacgaagtga ccttcttatc ggaccgcgtg tgtgggattt gcggttttgc
    18061 ccacagtgtg gcctatacca actcggttga aaatgcactg gggattgagg tgccgcaacg
    18121 agcacatact attcgctcga ttctgctgga agtcgagcgg ctacacagtc atttgctcaa
    18181 ccttggcctc tcctgccatt ttgttggttt tgataccggc tttatgcaat ttttccgcgt
    18241 gcgggaaaag tcgatgacga tggcggaatt gctgaccggg tcgagtaaaa cctacggtct
    18301 gaatctgatt ggtggtgttc gccgcgatat tctcaaagag caacgtctgc aaacgctgaa
    18361 actggtgcgc gagatgcgcg ccgacgtgtc ggagctggta gagatgctgc ttgctacgcc
    18421 gaatatggaa caacgcactc agggcattgg cattctcgac cgacaaatcg cccgtgatta
    18481 tagccctgta gggccgctga tccgcgcagt ggttttgccc gtgatttgcg ctttgatcac
    18541 ccctacgccg actacggcaa tattccaaaa acactgttta cctttaccgg cggcgatgtt
    18601 ttctcccgcg tgatggtccg tgtcaaagag acgtttgatt cgctggcaat gctggaattt
    18661 gccctcgaca acatgccgga taccccactg ctgaccgaag gctttagcta taaacctcac
    18721 gcattcgcgc tgggctttgt tgaagcgcca cgcggtgaag acgtgcactg gagcatgctc
    18781 ggtgataacc aaaaattgtt ccgctggcgc tgtcgtgccg ccacctacgc caactggccg
    18841 gtgttgcgtt acatgctgcg cggcaatacc gtttctgacg caccgctgat tatcggtagc
    18901 cttgatccct gctactcctg taccgaccgt gtgacgctgg tagatgtgcg caagcgccag
    18961 tcaaaaaccg tgctgtataa agagatcgaa cgctacggca ttgatcgtaa ccgttcgccg
    19021 ctgaagtaag gacagaagat gctgaagtta ctgaaaacta ttatgcgcgc cggaaccgcg
    19081 acggtgaaat atcccttcgc gccactggag gtcagcccag gctttcgcgg gaaaccggac
    19141 ctgatgccca gccaatgtat tgcctgcggt gcctgcgcct gtgcttgtcc ggcaaatgcg
    19201 ctgactatcc agaccgacga ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt
    19261 tgtatttact gcggacgttg tgaagaagtg tgcccgacca gagccatcca gcttaccaat
    19321 aactttgaac tgaccgtcac ccataaagcc gatctctata cccacgcgac gttccatcta
    19381 caacgttgca gccgttgcga acgcccgttt gccccgcaaa aaaccgtcgc actggctgct
    19441 gaattgttgg cacagcaaca aaatgctcca caaaaccgcg aaatgttatg ggcgcaagcg
    19501 agcgtctgcc cggagtgcaa acaacgcgcg acgctgatca acgacgatac agatgtaccg
    19561 ctggtggcta aggagcagct atgagtccag tgcttacgca atatgtcagc cagcccatca
    19621 cgctggacga gcaaacgcaa aagatgaagc ggcatttgct acaggatatc cgtcgctcgg
    19681 cttacgttta tcgcatcgat tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg
    19741 ccatcacccc cgtattcgac gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc
    19801 acgccgatat tttgttattt actggcacag tcacccgggc gatgcgtatg cctgcacttc
    19861 gggcgtatga gtctgccccc gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg
    19921 gcggcggtat tttccacgat ctctacagcg tctggggcgg cagcgacacc attgtcccca
    19981 ttgatgtttg gatccccggc tgcccgccaa caccggccgc caccattcac ggtttcgccg
    20041 tggcgctcgg tttgctgcaa cagaagattc acgcggtgga ttatcgcgat cccaccgggg
    20101 tgactatgca accgttgtgg ccgcagatcc cgccatcaca gcgtatcgcc attgagcgag
    20161 aagcgcggcg gctggcgggc tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc
    20221 atttaagcga cgatcctaca ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc
    20281 tacgtctcaa tagtatcgtt cagcaactct ttcgcgtact ccgggggtta catgactgaa
    20341 gagtgcgggg aaattgtttt ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag
    20401 atgccggaac acagctctca ggtaatgtat tactcgctgg ctatcggtca tcacgttggc
    20461 gtgattgatt gtctgaatgt cgccttccgc tgcccactga cggaatacga agattggctt
    20521 gcactggtcg aagaggagca agcccgacgt aagatgctgg gggtgatgac ttttggtgag
    20581 attgttattg acgccagcca caccgccctg ttgacccggg cattcgcgcc actggcagat
    20641 gacgcgacgt ctgtgtggca agcgcgtagc attcaattca ttcatctgtt ggatgaaatt
    20701 gtgctggaac cggctatcta tctgatggcc agaaaaattg cgtgagaagg atttctcatt
    20761 aataaggact gttgatggct atgtcagacg aagcgatgtt tgccccgccg caaggaataa
    20821 caattgaagc ggtaaacgga atgctcgcgg agcggttagc gcagaaacac ggcaaggcgt
    20881 ctttattacg cgccttcatc ccgctgccgc cgccgttcag cccggtacaa cttattgaac
    20941 tgcatgttct caaaagcaac ttctattacc gctaccatga tgatggcagc gatgtgacgg
    21001 caacaacaga gtatcagggc gagatggtcg attattcgcg ttacgccgtc cttctcggca
    21061 gtagtggaat ggcggagcta cgctttattc gtacccacgg cagccgtttt actccccagg
    21121 attgcacact gtttaactgg caggcacgca ttatcacccc ggttctgcaa tcatggctca
    21181 atgatgaagc acagcaggtg gcgctgcgtt tgctggagaa agatcgcgat catcatcggg
    21241 tactggtgga tatcactaat gcagtgctgt cacatcttga tctcgacgat ctgatcgctg
    21301 acgtcgctcg tgagatccat cattttttcg gtctggcttc agtcagtatg gtactgggcg
    21361 atcatcgaaa gaacgagaag ttcagcctgt ggtgcagcga tctttctgcc tcacattgtg
    21421 cgtgtctgcc acacaatatg cctggcgaca gtgtattgct gacacaaacg ctacaaaccc
    21481 gacaaccgac cttgacgcac cgtgcagatg atctgtttct ctggcaacgc gacccgttat
    21541 tactcttact tgcatctaac ggctgcgaat ctgcgctcct tataccgctt acctttggca
    21601 accatacacc gggtgcattg ttgctggcgc atacctcttc cactctcttt agtgaggaaa
    21661 actgccagct actacaacac atagccgatc gcatcgctat tgccgttggc aatgccgatg
    21721 cctgccgtag aatgaccgat ttgcaggaaa gtttgcagca agaaaaccac cagcttagca
    21781 agcagctcct ttcgaatctg ggcgtcggtg acattatcta tcaaagccag gcaatggaag
    21841 acctgctcca gcaggtagat attgtggcga agagcgacag tacggtgttg atttgcggtg
    21901 aaaccggaac cggcaaagag gtgatcgcca gagcgatcca tcaacttagc ccgcgacacg
    21961 acaagccgct ggtcaaaatc aactgcgctg ccatccccgc cagtcttctg gaaagtgagt
    22021 tattcggtca tgacaaaggg gcgtttactg gtgcgattaa tacccatcgt ggtcgttttg
    22081 aaattgccga tggcggcacg ttgtttctcg atgaaattgg cgatctgccg ttagaacttc
    22141 agcctaaact gctgcgcgta ttgcaggaac gggagattga gcgtctcggc gggagtagaa
    22201 cgatcccggt aaatgtcaga gtcattgccg ccaccaaccg tgatttgtgg caaatggttg
    22261 aagatcgcca gtttcgcagc gatctctttt atcgcctgaa tgtcttccca ctggaattgc
    22321 ctccgctgcg cgaccgtccg gaagatatcc ctcttttagc aaagcatttc acgcaaaaaa
    22381 tggcgcgcca tatgaatcgt tcaattgacg ccatcccgac cgaggcgcta cgccagttga
    22441 tgtcgtggga ctggccgggc aacgtgcgcg agctggaaaa cgtgattgag cgagcggtac
    22501 tgctgactcg cggtaacagt ctgaatttac atcttaatgt ccgacaaagc cgtttactgc
    22561 cgacgctaaa tgaagattca gcgcttcgca gttcaatggc gcagttgctg cacccgacga
    22621 cgccagagaa tgacgaagaa gaacgtcagc gcattgttca ggtattgcga gaaaccaatg
    22681 gcattgttgc cgggccccgt ggcgcggcga cacgattagg gatgaagcgt accacgctgc
    22741 tgtcacgaat gcagcgtctg ggaatctcgg ttcgcgaggt gttgtaatct gcttttgcag
    22801 gagtatgcat gagaaacaaa ctctctttcg acttgcagtt gagcgccaga aaagcggcaa
    22861 tcgctgaacg gattgccgcc cataaaattg cccgcagtaa agtgtcggtc tttttaatgg
    22921 cgatgtccgc tggcgtgttt atggcgatcg gatttacttt ttacctttcc gttatcgccg
    22981 atgccccgtc ttcataggca ttaacccatc tggtgggcgg cctttgcttt acacttggct
    23041 ttattttgct ggcggtttgc ggcaccagcc tgttcacctc gtcggtaatg acggtaatgg
    23101 caaaaagtcg gggcgttatt agttggcgaa cttggctgat taacgcactt ctggtggcct
    23161 gcggtaatct ggcaggtatt gcctgtttca gtttgttaat ctggttttcc gggctggtga
    23221 tgagtgaaaa cgcgatgtgg ggagtcgcga ttttacactg cgccgagggc aaaatgcatc
    23281 atacatttac tgaatctgtc agcctcggca ttatgtgcaa tctgatggtt tgcctggcgc
    23341 tgtggatgag ttattgcgga cgttcgttat gcgacaaaat cgtcgccatg attttgtcca
    23401 tcaccctgtt tgtcgccagt ggctttgagc actgtatcgc caatttgttt gtgattccgt
    23461 tcgccattgc cattcgccat ttcgccccta cctccttctg gcaactggcg cacagtagcg
    23521 cagacaattt tccggcactg acggtcagcc attttattac cgccaatctg ctcccggtga
    23581 tgctgggtaa tattatcggc ggtgcagtgc tggtgagtat ttgttatcgg gctatttatt
    23641 tacgtcagga atcctgaaaa atcagcccgg cgaaacagtt cgtcgggctg aagattattc
    23701 ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg tcgccaatgg
    23761 aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca ccgataaaat
    23821 aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta gcaggttgcc
    23881 gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag tacctgcgcc
    23941 aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc caatgtacgg
    24001 aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca gcccgaagag
    24061 caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt tgccgcggat
    24121 atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc cacggttacg
    24181 cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca ggaagaagac
    24241 catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca gcgaggcgag
    24301 ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat tttcggccat
    24361 tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg tggcggcaaa
    24421 gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc cctgttgcca
    24481 ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa ccaccaatac
    24541 aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg ttggccattc
    24601 cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac taaagaaaaa
    24661 gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg ggtcgctaaa
    24721 acggcggcga taccattgca tcaacatttc gagcataaag accttccctg aacctcaaga
    24781 gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc tttttatgac
    24841 ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac actcaaaggc
    24901 ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag aaatacagga
    24961 tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt gctgctatga
    25021 ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg gatatgggaa
    25081 cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac tattatgtcc
    25141 gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa tatattaatt
    25201 cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt catttttttc
    25261 tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg gtgctgcact
    25321 ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg gcgcacgaaa
    25381 tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa cgcagcgcgc
    25441 cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt ccgcaggcgg
    25501 ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc agtttcaccc
    25561 agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc tcgggattcg
    25621 atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt tactcctcgc
    25681 gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca gatgcccgca
    25741 accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc tatctggcga
    25801 aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt gatttgctgg
    25861 atgaatgggc gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt cgtgctttac
    25921 aggcgatgga agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg ttactggcgg
    25981 cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt gggcaaaaca
    26041 aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc aatccggtgt
    26101 tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa gcggccaata
    26161 ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat ttgctggcac
    26221 aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca gaaggttatg
    26281 cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt tcgcaggtga
    26341 aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg cgccagctgc
    26401 aggaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa caatgaccaa
    26461 acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc tgaacctgct
    26521 gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg ccgatgcggc
    26581 aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat tgatgcgcca
    26641 gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg aagaggcgct
    26701 gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg tcgcgaatgg
    26761 caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt aagatgtgct
    26821 ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat ccggcctacg
    26881 aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac aataaacacc
    26941 ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt tgcgcggtaa
    27001 tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc
    27061 gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta
    27121 actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc
    27181 agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc
    27241 cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg
    27301 ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag accagcgaca
    27361 aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc agcgggacat
    27421 agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc
    27481 gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt
    27541 tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt
    27601 cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca
    27661 ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc
    27721 aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc gtccagcacc
    27781 acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc gataccgacg
    27841 atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt cgcgccactg
    27901 acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact
    27961 ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag cagcagcgaa
    28021 acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag
    28081 gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta aacacgggtg
    28141 atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg
    28201 gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg atcgcgcagc
    28261 agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc
    28321 actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg
    28381 gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg
    28441 aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc caggaaacca
    28501 cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt
    28561 ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccatgccggc agctacgccc
    28621 gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg
    28681 tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg
    28741 cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt
    28801 gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga
    28861 tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag
    28921 acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt
    28981 gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc
    29041 tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt
    29101 aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg
    29161 aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca gttcgacgtc
    29221 cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca ccagaacttt
    29281 gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt
    29341 tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg
    29401 gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg
    29461 cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac gcagaattgg
    29521 cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc
    29581 gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac
    29641 ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag
    29701 cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg
    29761 ggcagccaca aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattattt
    29821 tcgttgactt tagtcaaaat gataacagtt tgagataaag ttattttata ttcaaatggt
    29881 tatgaaagaa gattattcca tccgaaaact aacctttgcc ctggcacaag tcttctttcg
    29941 ccgcgcgccc ggggaaaaga cgtgcaaaaa ggttgtgtaa aacagtctcg caaacgtttg
    30001 ctttccctgt tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc
    30061 caagcagtga ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg
    30121 ggtaatgctc tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg
    30181 atgggcggtc tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc
    30241 gtgctggttt ccggcactga cggcgtaggt accaagctgc gtctggcaat ggacttaaaa
    30301 cgtcacgaca ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa
    30361 ggtgcggaac cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc
    30421 gcttcagcgg tgatcagcgg catcgcggaa ggttgtctgc aatcgggctg ttctctggtg
    30481 ggtggcgaaa cggcagaaat gccggggatg tatcacggtg aagattacga tgtcgcgggt
    30541 ttctgcgtgg gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc
    30601 gatgtgctga ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc
    30661 aaaattcttg aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta
    30721 gccgatcatc tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa
    30781 aaggtcgatg tgcatgccat tgcgcacctg accggcggcg gcttctggga aaacattccg
    30841 cgcgtattgc cagataatac ccaggcagtg attgatgaat cttcctggca gtggccggaa
    30901 gtgttcaact ggctgcaaac ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc
    30961 aactgcggcg tcgggatgat tatcgccctg cctgctccgg aagtggacaa agccctcgcc
    31021 ctgctaaatg ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc
    31081 gaacaacgcg tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta
    31141 atttacaggc aattattgac gcctgtaaaa ccaacaaaat taaaggcacc gtacgggcag
    31201 ttttcagcaa taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa
    31261 cgcatacgct catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc
    31321 atgaaatcga catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca
    31381 gcccggcgtt tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc
    31441 cgaaatatcc cggattacac acccatcgtc aggcgctgga aaatggcgat gaagagcacg
    31501 gtacatcggt gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga
    31561 aagtcccggt atttgctggt gatacggaag atgacatcac cgcccgcgtg caaacccagg
    31621 aacacgctat ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg
    31681 aaaacgccgc gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt
    31741 aatgcccccg tagttaaagc gccagctctg ccgctggcgt ttttcaattc acctgtaaat
    31801 cgcaagctcc agcagttttt ttcccccttt tctggcatag ttggacatct gccaatattg
    31861 ctcgccataa tatccaggca gtgtcccgtg aataaaacgg agtaaaagtg gtaatgggtc
    31921 aggaaaagct atacatcgaa aaagagctca gttggttatc gttcaatgaa cgcgtgcttc
    31981 aggaagcggc ggacaaatct aacccgctga ttgaaaggat gcgtttcctg gggatctatt
    32041 ccaataacct tgatgagttc tataaagtcc gcttcgctga actgaagcga cgcatcatta
    32101 ttagcgaaga acaaggctcc aacgctcatt cccgccattt actgggcaaa attcagtccc
    32161 gggtgctgaa agccgatcag gaattcgacg gcctctacaa cgagctattg ctggagatgg
    32221 cgcgcaacca gatcttcctg attaatgaac gccagctctc cgtcaatcaa caaaactggc
    32281 tgcgtcatta ttttaagcag tatctgcgtc agcacattac gccgatttta atcaatcctg
    32341 acactgactt agtgcagttc ctgaaagatg attacaccta tctggcggtg gaaattatcc
    32401 gtagcgatag catccgttac gcgctgctgg agatcccatc agataaagtg ccgcgctttg
    32461 tgaatttacc gccagaagcg ccgcgtcgac gcaagccgat gattcttctg gataacattc
    32521 tgcgttactg ccttgatgat attttcaaag gcttctttga ttatgacgcg ctgaatgcct
    32581 attcaatgaa gatgacccgc gatgccgaat acgatttagt gcatgagatg gaagccagcc
    32641 tgatggagtt gatgtcttcc agtctcaagc agcgtttaac tgctgagccg gtgcgttttg
    32701 tttatcagcg cgatatgccc aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt
    32761 cccgctacga ctccatcgtc cccggcggtc gttatcataa ttttaaagac tttattaatt
    32821 tccccaatgt cggcaaagcc aatctggtga acaaaccact gccgcgttta cgccatattt
    32881 ggtttgataa agcccagttc cgcaatggtt ttgatgccat tcgcgaacgc gatgtgttgc
    32941 tctattatcc ttatcacacc tttgagcatg tgctggaact gctgcgtcag gcttcgttcg
    33001 acccgagcgt actggcgatt aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca
    33061 tcgactcgat gatccacgcc gcacataacg gtaagaaagt cactgtggtg gttgagttac
    33121 aggcgcgttt cgacgaagaa gccaacattc actgggcgaa gcgcctgacc gaagcaggcg
    33181 tgcacgttat cttctctgcg ccggggctga aaattcacgc caaactgttc ctgatttcac
    33241 gtaaagaaaa cggtgaagtg gtccgttacg cacacatcgg gaccgggaac tttaacgaaa
    33301 aaaccgcgcg tctttatact gactattcgt tgctgaccgc agatgcgcgt atcaccaacg
    33361 aagtacggcg ggtatttaac tttattgaaa acccataccg cccggtgaca tttgattatt
    33421 taatggtgtc accgcaaaac tctcgccgtc tgttatatga aatggtagac cgcgaaatcg
    33481 ccaacgcgca gcaagggctg cccagtggta tcaccctgaa gctaaataac cttgtcgata
    33541 aaggcctggt tgatcgtctg tatgcggcct ccagctccgg cgtaccggtt aatctgctgg
    33601 ttcgcggaat gtgttcgctg atccccaatc tggaaggcat tagcgacaac attcgtgcca
    33661 tcagtattgt tgaccgttac cttgaacatg accgggttta tatttttgaa aatggcggcg
    33721 ataaaaaggt ctacctttct tccgccgact ggatgacgcg caatattgat tatcgtattg
    33781 aagtggcgac accgctgctc gatccgcgcc tgaagcagcg ggtgctggac atcatcgaca
    33841 tattgttcag cgatacggtc aaagcacgtt atatcgataa agaactcagt aatcgctacg
    33901 ttccccgcgg caatcgccgc aaagtacggg cgcagttggc gatttacgac tacatcaaat
    33961 cactcgaaca acctgaataa ccctatgcca atacacgata aatcccctcg tccgcaggag
    34021 tttgctgcgg tcgatcttgg ttcaaacagt tttcacatgg tcatagcccg tgtggtagat
    34081 ggtgccatgc agattattgg ccgcctgaaa cagcgggtgc atctggcgga cggcctgggg
    34141 ccagataata tgttgagtga agaggcaatg acgcgcggtt tgaactgtct gtcgctgttt
    34201 gccgaacggc tacaagggtt ttctcctgcc agcgtctgta tagtcggcac ccatacgctg
    34261 cgtcaggcgc tgaacgccac tgactttctg aaacgcgcgg aaaaggtcat tccctacccg
    34321 attgaaatta tttccggtaa tgaagaagcc cgtctgattt ttatgggcgt ggaacatacc
    34381 caaccggaaa aaggtcgcaa actggttatt gatattggcg gcggatctac ggaactggtg
    34441 attggtgaaa atttcgaacc tattctcgtt gaaagccgcc ggatgggttg tgtcagcttt
    34501 gcccagcttt attttcctgg cggggtcatc aataaagaga attttcagcg cgctcgcatg
    34561 gcggcagcac aaaaactgga aactttaacc tggcaattcc gtattcaggg ctggaacgtt
    34621 gcaatgggcg cttccggtac cataaaagcc gcccatgaag tgttaatgga aatgggcgag
    34681 aaagacggga taattacccc ggaacgtctg gaaaaactgg taaaagaaat tttacgtcac
    34741 cgtaatttcg catcgctgag tttaccgggt ctttccgaag agcggaaaac agtcttcgtt
    34801 cctggactgg cgattttatg tggtgtgttt gatgctttag ccatccgtga actgcgcctt
    34861 tctgacgggg cgttacgcga aggcgtactg tatgaaatgg aaggacgttt ccgtcatcag
    34921 gatgtgcgta gtcgcaccgc cagcagcctc gccaaccagt atcacataga cagcgaacag
    34981 gcccgacgag tgctggatac cactatgcaa atgtacgaac agtggcggga acagcaaccg
    35041 aagctggcgc atccgcaact ggaggcgcta ctgcgatggg ctgccatgct gcatgaggtc
    35101 gggttgaata tcaaccacag cggtttgcat cgccactccg cttatattct gcaaaacagt
    35161 gacttgccgg gttttaatca ggaacagcag ctgatgatgg cgacactggt gcgctatcac
    35221 cgtaaagcga ttaagctcga cgatctgccg cgctttacct tgtttaagaa gaaacagttc
    35281 ctgccactga tacagctatt gcgccttggc gtattactaa acaatcaacg tcaggcaacc
    35341 accacaccgc caacattgac actgattact gatgacagtc actggacact gcgtttcccg
    35401 catgactggt ttagtcagaa tgcgctggta ctgcttgatc tggaaaagga gcaagaatac
    35461 tgggaaggcg tggctggctg gcggttgaaa attgaagaag aaagtacccc agaaatcgcc
    35521 gcttaatgct ttgcgggccg acgagaaatg tcggcccgca ttattcaggc actttcgcga
    35581 atgggttcga tttcattcag cgtatcaatt aacggttgcg gcttaccaat aagataaccc
    35641 tgcatataat cgatccccaa agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac
    35701 tctgccacta cccgcatttt cttcattcgc gccagatggc aaatcgacgc cacaatctga
    35761 taatccagac tatttgacac aatattgcgg ataaagctgc cgtcaatttt aagcagatcg
    35821 gcattcacat ttttaagccg tgcatagctg gcgtagccgg tgccgaaatc atcaatcgca
    35881 atctggcagc ctaattcctg aagatgctgc aaggtgattt gcgcctgctt aacattggtc
    35941 agagcattac tttcggtgac ttcaaaaata agttgccacg cttcaatctg atatttagcc
    36001 agcaactgac tgacttcaac aggaaaacga gcctgacata ccgaggttgg agacagatta
    36061 atagcaaaac ggtgagcggg catcttcgct ctgttttcag ccataaattg cagcgtatgc
    36121 tcaatgaccc acatgtcgat actcgacgat aaaccaaatt cgtgcgcgac cggcaagaag
    36181 ctaccggggc cgatcagttc atcattctca cctttcatgc gcagaagaat ttcatggtaa
    36241 acatcaccac gcataccggt aatcggctgg gccatcagga aaaaatggtt gtgttccagc
    36301 gcctgctgta gtcgattcat catcgcgact ttatctttca attcgcgttg caaatacatt
    36361 gccccgcaac gctgcatatt ttccggggcg ttggtcacga tggaaagttc ggcgaccgta
    36421 tttagctctc ccagcagcag gtagatatga ttcactggcg agcgcacata gcagtaactg
    36481 acgccaatct gcggttgcat cggcatccca tcccaaaaga aacgaaattg cttgagatga
    36541 ctatccagtg cggtaatgcg ctcctggtgc gattctgtat tcagtcgcag cgcgagatcg
    36601 ttacccgaaa gctgataaac atcttcaccc ggttccagca agggtgacag ccagtgagaa
    36661 agtttttgct t
//
LOCUS       DAECOD010000028        36622 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.028, whole genome shotgun sequence.
ACCESSION   DAECOD010000028 DAECOD010000000
VERSION     DAECOD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.028"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="KKO15_001488"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="KKO15_001488"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBD6360327.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="KKO15_001489"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="KKO15_001489"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBD6360328.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKIFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4116..4358
                     /locus_tag="KKO15_001490"
     CDS             4116..4358
                     /locus_tag="KKO15_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360329.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4472..5110
                     /locus_tag="KKO15_001491"
     CDS             4472..5110
                     /locus_tag="KKO15_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD6360330.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5107..5844
                     /locus_tag="KKO15_001492"
     CDS             5107..5844
                     /locus_tag="KKO15_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD6360331.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5844..7940
                     /locus_tag="KKO15_001493"
     CDS             5844..7940
                     /locus_tag="KKO15_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD6360332.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7987..8265)
                     /locus_tag="KKO15_001494"
     CDS             complement(7987..8265)
                     /locus_tag="KKO15_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360333.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8266..8409
                     /locus_tag="KKO15_001495"
     CDS             8266..8409
                     /locus_tag="KKO15_001495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360334.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8535..8945
                     /gene="psiE"
                     /locus_tag="KKO15_001496"
     CDS             8535..8945
                     /gene="psiE"
                     /locus_tag="KKO15_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBD6360335.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8989..10464)
                     /gene="xylE"
                     /locus_tag="KKO15_001497"
     CDS             complement(8989..10464)
                     /gene="xylE"
                     /locus_tag="KKO15_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBD6360336.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10836..11726)
                     /gene="malG"
                     /locus_tag="KKO15_001498"
     CDS             complement(10836..11726)
                     /gene="malG"
                     /locus_tag="KKO15_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBD6360337.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11741..13285)
                     /gene="malF"
                     /locus_tag="KKO15_001499"
     CDS             complement(11741..13285)
                     /gene="malF"
                     /locus_tag="KKO15_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBD6360338.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13439..14629)
                     /gene="malE"
                     /locus_tag="KKO15_001500"
     CDS             complement(13439..14629)
                     /gene="malE"
                     /locus_tag="KKO15_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBD6360339.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14994..16109
                     /gene="malK"
                     /locus_tag="KKO15_001501"
     CDS             14994..16109
                     /gene="malK"
                     /locus_tag="KKO15_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBD6360340.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16181..17521
                     /gene="lamB"
                     /locus_tag="KKO15_001502"
     CDS             16181..17521
                     /gene="lamB"
                     /locus_tag="KKO15_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBD6360341.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17673..18593
                     /gene="malM"
                     /locus_tag="KKO15_001503"
     CDS             17673..18593
                     /gene="malM"
                     /locus_tag="KKO15_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBD6360342.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSRTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            18822..20402
                     /locus_tag="KKO15_001504"
                     /pseudo
     CDS             18822..20402
                     /locus_tag="KKO15_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            20625..21122
                     /gene="ubiC"
                     /locus_tag="KKO15_001505"
     CDS             20625..21122
                     /gene="ubiC"
                     /locus_tag="KKO15_001505"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBD6360343.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            21135..22007
                     /gene="ubiA"
                     /locus_tag="KKO15_001506"
     CDS             21135..22007
                     /gene="ubiA"
                     /locus_tag="KKO15_001506"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBD6360344.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(22162..24585)
                     /gene="plsB"
                     /locus_tag="KKO15_001507"
     CDS             complement(22162..24585)
                     /gene="plsB"
                     /locus_tag="KKO15_001507"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBD6360345.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            24756..25124
                     /gene="dgkA"
                     /locus_tag="KKO15_001508"
     CDS             24756..25124
                     /gene="dgkA"
                     /locus_tag="KKO15_001508"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBD6360346.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            25234..25842
                     /gene="lexA"
                     /locus_tag="KKO15_001509"
     CDS             25234..25842
                     /gene="lexA"
                     /locus_tag="KKO15_001509"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBD6360347.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            25915..27240
                     /gene="dinF"
                     /locus_tag="KKO15_001510"
     CDS             25915..27240
                     /gene="dinF"
                     /locus_tag="KKO15_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBD6360348.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSIAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            27356..27565
                     /locus_tag="KKO15_001511"
     CDS             27356..27565
                     /locus_tag="KKO15_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBD6360349.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(27607..28122)
                     /gene="zur"
                     /locus_tag="KKO15_001512"
     CDS             complement(27607..28122)
                     /gene="zur"
                     /locus_tag="KKO15_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBD6360350.1"
                     /translation="MGKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            28440..29425
                     /locus_tag="KKO15_001513"
                     /pseudo
     CDS             28440..29425
                     /locus_tag="KKO15_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            29788..30825
                     /gene="dusA"
                     /locus_tag="KKO15_001514"
     CDS             29788..30825
                     /gene="dusA"
                     /locus_tag="KKO15_001514"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBD6360351.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            30959..31201
                     /gene="pspG"
                     /locus_tag="KKO15_001515"
     CDS             30959..31201
                     /gene="pspG"
                     /locus_tag="KKO15_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBD6360352.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(31367..32350)
                     /locus_tag="KKO15_001516"
     CDS             complement(31367..32350)
                     /locus_tag="KKO15_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBD6360353.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            32433..33848
                     /gene="dnaB"
                     /locus_tag="KKO15_001517"
     CDS             32433..33848
                     /gene="dnaB"
                     /locus_tag="KKO15_001517"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD6360354.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            33901..34980
                     /gene="alr"
                     /locus_tag="KKO15_001518"
     CDS             33901..34980
                     /gene="alr"
                     /locus_tag="KKO15_001518"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBD6360355.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            35233..36426
                     /gene="tyrB"
                     /locus_tag="KKO15_001519"
     CDS             35233..36426
                     /gene="tyrB"
                     /locus_tag="KKO15_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBD6360356.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         9086 a         8957 c         9470 g         9109 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaat cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgcagatta attacttttg tttttatttt aagcttatga tttttattgt tatttaaata
     3961 taagttgaaa cttatatttg atattcattc caattatcct aaaactccat cgctaattcc
     4021 ccgcgccgta attcgcatgc tttagttgtg tatactcgat cccgcccgaa atgtttttgg
     4081 gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa aaaagttctg tatggcattt
     4141 ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc acctgtacag gtgggcgaag
     4201 cggcagagtc ggcagcaacg tcggtttcgg cggggagttc ctccgcgacc agcgtcagca
     4261 ccgtaagctc ggcggtgggt gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata
     4321 ccgggaccat cacaaccacg accaccagta cccagtaata aagtatgtat ccccaaaata
     4381 atttgagtca ttgcatctgt ggcttgaagt atgaagggat ttaccataac cacactccgg
     4441 tgtggttatt ctgcccctct ggagaagagt cgtgaagcga cctgcactca ttcttatctg
     4501 cctgctatta caggcctgtt cagccacgac taaagagctg ggcaattcac tgtgggacag
     4561 tctgttcggc acgccaggcg tacagctgac ggacgatgat attcaaaata tgccctacgc
     4621 cagccagtac atgcagctta atggcgggcc gcaattgttt gtggtgctgg ccttcgctga
     4681 agacggacaa caaaaatggg tcactcagga tcaagccact cttgtcacac aacatggtcg
     4741 tctggtgaag actttgcttg gcggcgacaa cctgattgaa gtgaataacc ttgccgccga
     4801 cccgctgatt aaacccgcac aaattgttga tggtgcaaca tggacgcgca cgatgggctg
     4861 gaccgagtac cagcaggtac gctacgccac cgcacgctca gtcttcaaat gggatggcac
     4921 cgataccgtc aaggtcggca gcgatgaaac cctggttcgc gtgctggacg aagaagtctc
     4981 caccgaccag gcgcgctggc ataaccgcta ctggatcgac agcgaagggc aaattcgcca
     5041 gtcggaacag tatctcggcg cggattattt cccggtgaaa accactctca tcaaggcggc
     5101 aaaacaatga ttaaacaaac tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc
     5161 ttcgcggcag gaaccgtcaa ggtgttcagc aatggcagca gtgaggccaa aacgctaacg
     5221 ggcgcagagc atttaatcga tctggtaggg caacctcgac tggcaaacag ctggtggcct
     5281 ggtgcggtga ttagcgaaga gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg
     5341 ctgacacggc tggcagaaca gggcgcagat tccagcaccg acgatgccgc cgccattaac
     5401 gccttacgcc agcaaatcca ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat
     5461 cccgatatcg tccgcgttgc cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg
     5521 tgggtcggac caccaccgtc cacggtcacg ttgttcgggc ttatcagcca tcctggcaat
     5581 cagccattca ctccaggtcg cgacgtggcg agctatctct ctgaccagag cctgctcagc
     5641 ggtgcggatc gcagctacgc gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg
     5701 gtggcttact ggaacaagcg tcacgtagag ccgatgcccg gcagcattat ttatgttggc
     5761 ctcgcagatt ccgtctggag tgagacgcct gatgccctta acgccgacat tcttcagact
     5821 ctgacgcagc ggatacccca ataatgaaaa aaagacatct gcttagctta ctggcgctgg
     5881 gcattagcac agcttgctac ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg
     5941 atttcggtgg cgtaggatta ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt
     6001 tgagtctgaa ctatcgcgat aacgatcagt accgttatta ctcagcttca gtgcaactct
     6061 tcccgtggct ggaaacaacg ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg
     6121 tcgaagcgtt ctctggcgat caaacgtata aagataaagc cttcgatctc aaactgcgtt
     6181 tgtgggaaga gagttactgg ctgccgcaag tggcggttgg tgcgcgggat atcggcggta
     6241 cggggctgtt tgatgcggaa tatcttgttg ccagcaaagc ctgggggccg ttcgatttta
     6301 cgctcggcct gggctgggga tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt
     6361 cagccagtga taaatattgc tatcgcgata acagctacaa acaggcggga tctatcgacg
     6421 gtagccagat gttccacggt cctgcctcac tgtttggcgg cgtggaatac cagacgccct
     6481 ggcaaccgct gcgtctgaaa ctggagtatg aaggtaataa ttatcagcag gattttgccg
     6541 ggaagctgga gcaaaaaagt aagtttaacg tcggggcgat ttatcgcgtt accgattggg
     6601 ccgacgttaa ccttagctat gaacgtggca acacctttat gtttggcgtc acgctgcgca
     6661 ccaactttaa cgatctgcgc ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc
     6721 agccgcagga tgccattttg cagcattcgg tggtggcgaa tcagttaacg ttgctgaaat
     6781 acaacgccgg acttgccgat ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg
     6841 gcgagcaggt gaaatatcgt gattcgcgcg aagggatcat ccgtgctaat cggatcgtga
     6901 tgaacgatct gccggatggg atcaaaacga tccgcattac ggaaaatcgc cttaacatgc
     6961 cgcaagtgac gacggaaacc gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt
     7021 tgggccacga aacgacgctg gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg
     7081 agcagggctg gtatatcgac aaatcacgct ttgatttcca tatcgatccg gtgctgaacc
     7141 agtcggtcgg tggcccggaa aacttttaca tgtatcagct gggcgtgatg ggaacggcag
     7201 atttgtggct gacggaccat ctactgacca ccggcagcct gtttgcaaat cttgccaaca
     7261 actacgacaa gtttaactac accaatccac cgcaggactc gcacttaccg cgcgttcgta
     7321 cccatgtgcg cgagtatgtg cagaacgatg tctatgtgaa taacctgcaa gccaactact
     7381 tccagcatct gggcaacggc ttctacggtc aggtctatgg aggttatctc gaaaccatgt
     7441 ttggcggtgc gggggccgaa gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc
     7501 tggatgccaa ctacgttaaa cagcgcgact ggcgtagtgc aaaagatatg atgaaattca
     7561 ccgactacag cgtgaaaacc ggacatctga ccgcctactg gacgccatct ttcgcccagg
     7621 acgtgttggt gaaagccagc gtcgggcagt atctggcagg ggataaaggc ggcacgctgg
     7681 agatcgctaa acgctttgac agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg
     7741 tttcgaaaga ggagtacggc gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt
     7801 tggatctctt ctcgtctggc ccgacacgca gccgtgcggc gattggctgg acgccgctga
     7861 cgcgtgacgg tggtcagcaa cttgggcgta agttccagtt gtatgacatg accagcgacc
     7921 gtagcgtaaa tttccgctaa gtcatgggaa aggtgccagt tttcgcactc actggcaccc
     7981 tcattcttaa tgacctgttg agccatcagg aacgcagttc acccccggtt tacgatctgg
     8041 tgtgtaaagg cgaaaacatt ctgtcttcct gtccgtcgat atcggttgca ggtctggttg
     8101 cctgagcgac tgggccatca ttgggctgga gacattgctt tgcggtaggt cggctttatc
     8161 agcagtaccg agcggaccag cataagcagg aagaacagag actgataaca tcaaagcagc
     8221 aaaataaggc ttcattttta ccacctttat caggttacgt ttcatttgtt ccagaggaac
     8281 attgtcgatt tttcgcgcat tgctggtggc tgggaatcac ctgaatgggt gatttttgaa
     8341 ttaccggctt tggtgcggtt tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc
     8401 tacgggtagg tatatccggc tttggtggag gcgcgctcca aatccaggtt gaacaaaaca
     8461 tacacaaaaa atatagatct ccgtcacatt tttgcgttat acaggaagct cgccactgtg
     8521 aaggaggtac tgctatgacg tcactctctc gtccgcgcgt ggagtttatc tccaccattt
     8581 tgcagaccgt actcaatctt ggcctgctgt gcctcggcct gattttggtt gtcttcctcg
     8641 gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg
     8701 agctggtaga aggactggtg gtttactttc tctatttcga atttatcgcg ctgattgtga
     8761 agtactttca gtccggtttt cacttcccgc tacgttactt tgtctatatt gggatcaccg
     8821 ccattgtgcg cttgatcatc gtcgatcata aatcgccact ggatgtgttg atctactcgg
     8881 ctgcgatcct gttactggtg atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg
     8941 agtaaaaaga cagcacgccg aaatggcgcg gcgtgctgga caggaagatt acagcgtagc
     9001 agtttgttgt gttttcttcg tttccggttc ccagagcgct tccagctcct caagggtttt
     9061 acctttggtt tccgggacaa atttccacat aaacagtgct gccagaacgc ccatacaacc
     9121 gtaaatccag taggagaaac cgttgtggaa atgggccacc agccaggagt ttttgtccat
     9181 catcgggaag gtccaggaga cgaagtagtt cgccagccac tgggccgcca ccgcgattgc
     9241 cagcgcttta ccacgaatag cattcgggaa gatttccgac agcagtaccc agcataccgg
     9301 accccaggac atggcaaagg cggcaacata gaacagcatc gacagtagcg ccacaatacc
     9361 cgatgcctga gtgtaaaacg cggtaccgag gctaaacata ccgattgcca ttccgagtgc
     9421 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     9481 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     9541 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     9601 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     9661 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     9721 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     9781 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     9841 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     9901 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     9961 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
    10021 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
    10081 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
    10141 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
    10201 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
    10261 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
    10321 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
    10381 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
    10441 actggaatta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
    10501 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
    10561 cgtaatgtct tttctgtgat cttaattgtg ataattatca aaaattgatg aaaacaatac
    10621 tattgccgtg actcagagca cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa
    10681 aaataataag ttctatgatg ggaatattaa ccggcgaacg attcagattg cagacgaaag
    10741 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
    10801 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
    10861 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
    10921 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
    10981 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
    11041 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
    11101 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
    11161 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
    11221 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
    11281 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
    11341 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
    11401 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc agcgaccttt
    11461 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
    11521 tgttcaacgc taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
    11581 aggctaccgg tcgcgaagtt tccctgacgc agcgagatag cgacgaccat cagcagcggg
    11641 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
    11701 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
    11761 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
    11821 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatacgg taggtgtagt
    11881 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
    11941 tggttaacag ctgaatcagc acgaagttgt taaagttaaa ggcgaagctg gcgatcatca
    12001 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaacggac
    12061 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
    12121 tgcagaggat catcatgtac ggataaccca gccaggtatt gacgataatc agcatcgtgc
    12181 gagcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca
    12241 tgttgatttc accgaagctc tggttaaaca atcctttgaa aatcaagatt gaaatgaacg
    12301 atggcaccgc gtagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
    12361 cccactgcac cagacatgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
    12421 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
    12481 taaagacgcg ggtaaagttt ttccagccgg tggtcacggt gtaaccgggg cttagctttt
    12541 catcccccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
    12601 gatatttcac gccactctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
    12661 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
    12721 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
    12781 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
    12841 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
    12901 cgcccgccgg gtaaagacca aagttatagg ttttgcctgc ttgccaggag cgatctaaca
    12961 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
    13021 tggcgatggt gcagaccaga gggaagagga cgaataatcc cattccagcc attcccgggt
    13081 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
    13141 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
    13201 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
    13261 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
    13321 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
    13381 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
    13441 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
    13501 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgttcg
    13561 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
    13621 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
    13681 ccagaccttc atcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
    13741 gactggcggc gttaataccg gtgctcagca cgccaacgaa cggtttagat ggttgaccct
    13801 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
    13861 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
    13921 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
    13981 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
    14041 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
    14101 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
    14161 cttcccaggt ttttggcggg ttcggcagca ggtctttgtt ataaatcagc gataacgctt
    14221 caacagcgat tgggtaagca atcagcttgc cgttgtaacg tacggcatcc catgtaaacg
    14281 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
    14341 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
    14401 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
    14461 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
    14521 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
    14581 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
    14641 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacggcaac
    14701 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
    14761 attggcggta atgtggggat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
    14821 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
    14881 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
    14941 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
    15001 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
    15061 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
    15121 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
    15181 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
    15241 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
    15301 ctggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
    15361 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg
    15421 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
    15481 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
    15541 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
    15601 aaatcgtggt gctggacgcc ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc
    15661 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
    15721 cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    15781 gtcaacaagt ctggctgcct gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    15841 tgggtattcg cccggaacat ctactgccga gtgatatcgc tgacgtcatc cttgagggtg
    15901 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ctatatccag atcccttcca
    15961 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    16021 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    16081 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    16141 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    16201 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    16261 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    16321 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    16381 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    16441 taacgtggcc tattccgtcg cgcaacagaa tgactgggaa gctaccgatc cggccttccg
    16501 tgaagcaaac gtgcagggaa aaaacctgat cgaatggctg ccaggttcca ccatctgggc
    16561 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    16621 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    16681 agcaacccgc tcctctgaag ctggtggttc ttcctctttt gccagcaaca atatttatga
    16741 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    16801 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    16861 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    16921 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    16981 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    17041 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    17101 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    17161 caccaagtgg tggaccgttg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    17221 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    17281 gtacaaaatt accctcgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    17341 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttacg actacaacgg
    17401 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    17461 cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    17521 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    17581 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    17641 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    17701 tctgtttatc agcagggtta ctggcaagcg cgcctggaat tagccttgcc gatgttaact
    17761 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    17821 cctggacacc ggtcgatcaa tctagaaccc agaccaccca actggcgacc ggcggccaac
    17881 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    17941 gcgaactgcc cctgacgctg accagcgaag tgaacaaaca aaccagcgtt tttgcgccga
    18001 acgtgctgat tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca
    18061 cctaccagga actaggcgtg atgagtgcag agcggctgga aggcgttatg cgcctgacac
    18121 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    18181 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    18241 cggatattcc cgatccggtt gctcgtcata ccagcgatgg cttactgaaa ctgaaagtca
    18301 aaacgaactc cagctccagc gtgttggtag gaccactatt tggttcttcc gctccagctc
    18361 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acctgctccg gtgccggtga
    18421 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    18481 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    18541 gatcgacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    18601 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttcc
    18661 ttcctgggat atgagcgatt ttttatagta cttcacttct tcttcactaa gaatgtccat
    18721 tatttcaatg cattatcaga gattcttttc ctttcgccgg tagtgtctgg acgctcaggc
    18781 tacttttcca ggttatttta tttctgtatt acagaggttt tatgataggt catatcacaa
    18841 attctggcgg caataactct ttgatgaaac atgatgtggt gcaaggaaat aatatagtag
    18901 atcttgattt actacgtaat ttcaatgggg tgacaggttt aaacagagat tactttattt
    18961 atatcagtga tatattttta aatataaaac aacggaacga aaaaaatcat gcaataaata
    19021 tgttacgtga agtctcaatt agtaatgata ctataagtgt aaaattctac agaaatgaaa
    19081 aaatagaatg cgcttgtaat tttctgatgg ataaagatgc gcaggggtat atcgacctgt
    19141 ctgattttga tttaacaaat tgtcatttta aaggtgacgt tatttctaag gtgtcttttt
    19201 tatcatcaaa tctacaacat gtaactttcg aatgtaaaga aattgagaat tgcaatttta
    19261 ctaaggcaac agttgataat gtcatattta aatgtcgacg tttacacaat gtgatttttc
    19321 tcaaaacgag tggcgaatgt gttgatttta gccaaaatat tcttgataca gttgacttct
    19381 cgcagagtca acttggtcat agtaattttc gcgaatgtca gattagaaat tcaaacttcg
    19441 ataattgtta tctttacgct tcgcacttca ccagagcaga gtttctgtct gccaaagaaa
    19501 tatcatttat taaatcgaat ttgacagctg ttatgtttga tcatgtgcga atgtcgacag
    19561 ggaattttta agattgcatt acagaacaat tggaattaac tattgattat tcagatatat
    19621 ttgggaatga agatctcgat ggttatatca ataacattat aaaaatgatt gatacattgc
    19681 cagataatgc aatgatattg aaatccgttc tggcagtaaa actggtgatg caattaaaaa
    19741 ttctcaatat tgttaataaa aactttatta agaatatgaa gaaaacattt agccattgtc
    19801 cttatataaa agatccaatt atacgtagtt atatacattc tggggaagat gacaagttcg
    19861 ataattttat gcgtcaacat cgattcagta aggtgaattt cgatacccaa cagatgatcc
    19921 attttattaa cagatttaat atgaataaag ggctggttga taaaaataac aattttttta
    19981 tccaacttat cgatcaggcc ttacgatcaa cggatgatat gatcaaagca aatgcctggt
    20041 atctttataa agagtggatt cgtagtgatg atgtttcacc tatatttata gaaactgaag
    20101 aaaagttaag aacctttaac actaataaat taacacgaaa cgataatata tttatcctgt
    20161 tctcctctgt cgatgatggg cctgttatgg tggtaagctc ccagcgctta catgatatgt
    20221 tgaatcctac aaaagatacc aattggaatt ccacgtgtat ctataaatcc agacataaga
    20281 tgttgcctat taatcttact caggaaacac ttttcagctc caaatctcat ggtaaatatg
    20341 cgcttttccc aatttttact gcgagttggc gagctactcg tataaagaat aaaggtattt
    20401 aagtaaagga aaacatcacc gttcctagca tcatgggcgg tgatgcccct atggttgccc
    20461 tcgccagcac gggcatcggc aaagcgtaag gttcaacagc gttttaccac ttcatgcgat
    20521 tgttgcgttt ttgttgcgca ttagatcact taatttgctt tacatctccc gtaaacactt
    20581 ttctgcgata caatgccttt acgttatgta acggagagtt cggcatgtca caccccgcgt
    20641 taacgcaact gcgtgcgctg cgctatttta aagagatccc tgccctggat ccgcaactgc
    20701 tcgactggct gttgctggag gattccatga caaaacgttt tgaacagcag ggaaaaacgg
    20761 tgagtgtgac gatgatccgc gaggggtttg tcgagcagaa tgaaatcccc gaagaactgc
    20821 cgctgctgcc gaaagagtct cgttactggt tacgtgaaat tttgttatgt gccgatggtg
    20881 aaccgtggct tgccggtcgt accgtcgttc ctgtatcaac attaagcggg ccggaactag
    20941 cgttacaaaa attgggtaaa acgccgttag gacgctatct gttcacatca tcgacactaa
    21001 cccgggattt tatcgagata ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc
    21061 gattaagcgg taaaccgctg ttgctaacag aactgttttt accggcgtca ccgttgtact
    21121 aagaggaaaa aaatatggag tggagtctga cgcagaataa gctgctggcg tttcatcgct
    21181 taatgcgtac ggataagcca attggcgcgt tactgctgct ctggccaaca ttatgggcgt
    21241 tgtgggtggc gacaccgggc gttcctcagc tctggatcat ggcggtgttt gtcgcgggtg
    21301 tctggctgat gcgcgctgct ggttgcgtgg tgaatgatta tgctgatcgc aagtttgatg
    21361 gtcatgttaa gcgcacggcg aaccgaccac ttcccagcgg cgcggtaaca gagaaagagg
    21421 cgcgcgcgct gtttgtagtg ctggtactga tttcgttttt actggttctg acgctgaata
    21481 cgatgaccat tctgttgtcg attgccgctc tggcattggc gtgggtgtac ccgtttatga
    21541 agcggtatac ccatctcccg caagtggtgc tgggcgcggc gtttggctgg tcgattccaa
    21601 tggcttttgc cgctgtgagt gagtcggtgc cattgagttg ctggttaatg ttcctcgcca
    21661 atattctctg ggcggtggct tacgacacgc agtatgcgat ggttgaccgc gatgatgatg
    21721 tgaagattgg cattaaatcc acggcaatcc tgttcggcca atacgataaa ttgattattg
    21781 gtattttgca gattggcgta ctggcactga tggcgatcat cggtgagtta aatggcttag
    21841 gctggggata ttactggtca attgtggtgg ctggcgcgct gtttgtttat caacaaaaac
    21901 tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat aactatgttg
    21961 gtctggtact gtttttagga ctggcaatga gttactggca tttctgatga tgtaaaaaag
    22021 ccggatgatc atccggcttt cttctgggtt gcctgatgcg ttgcgcttct caggcctacc
    22081 cggtgaatcg caatttattg aatttgcagg tcatggtagg ccggataagg cgttttcgcc
    22141 gcatccggca attctctctg attacccttc gccctgcgtc gcactctcaa tcgtcaaacg
    22201 cacgtctgat gtaatcaact ccgccagcaa ctgataaacc ttcatcgttt ctgctggttc
    22261 ggcatcgccg ctatcgctga tatacccttc atcacgcagc gtcagcacca gagaactgaa
    22321 caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc
    22381 aacggtgcgg ctctctttct ccagcgtacc gcggttgatc gacgggttgg cactcaacaa
    22441 ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag
    22501 ggtgcgagaa tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg
    22561 acgttgcatc tcatttgcca gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg
    22621 caggaacagc tccgctttca gcattgggta aagcacattg acgtgctcca tcaatacgtc
    22681 gcgggagatg tggcgatgct gggtgacaat tgccgccatc agcgaaggca gcaccaacat
    22741 atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct tgctcgcgtg gcagaatgat
    22801 gatgtcgccg atagtgtctt tctcgacttc aaacttgttc atttgcagcg cgtgatcgat
    22861 aagctcgctg gcgctggctg aaggaacggt agagtccgtg gagtaaggta cgttgcgcat
    22921 cagatccagg tagcagttga gttgctcggt taactgctcg cgggtgagtg agcgctgacg
    22981 tgatgccagt agcgcagtac agcacaggtt catggcgttt gccgcgcctg cgttgttaat
    23041 gcgtaccatc agatcggcag caatattatt gaccgtcggc gttaaccagg ccggacgcac
    23101 cgcttcgatg ggatcgatag attcacgcca gtctggtacg tgctggttaa ggtaggtcat
    23161 caacggcatt ggttcaccga agttgacgta accctgaccg agattacgca gcttgcttaa
    23221 accgcgcagc atctgcggca ggctctcttt ctctttcgtc gcgccgcgca gttctttggc
    23281 gtaagtaccc acttccatga cgtgctcata accgatatag atcggaatca gcgtaatcgg
    23341 acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc
    23401 cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag tactcgacgg aataaccacg
    23461 gctgaacagt tcgccgagat actcacggaa aacggtggaa taaagtttat tgcctttaaa
    23521 agtacggcga ataaagaacg cccccagacg gcggaaaatc ggcccggccg gccagaagtt
    23581 caggttgatc ccggcggcga tatgcggcgg caccagcccc tggtggtaca gcacgtaaga
    23641 aagtagcagg tagtccatgt gactgcggtg gcaaggcaca tacaccagct catgaccgtc
    23701 gtgggccagc tggcgaacgc gctcggcgtt atggacgttg atgccctgat aaaatcggtt
    23761 ccaggtgaag cccagaatgc ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc
    23821 aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga tttttttgct
    23881 gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag gcgagcagct tattaaacag
    23941 atcctgacga gcaggcagac gtgggcctac ggcagccaga cgttgacggg caaagtgcat
    24001 acgcgccacg cgcgccagtt tctgagcgat agttttatcc gtgccgtgtt catccgccat
    24061 acggcgcagc gacactgacg gcgagaaacg cacaaaactg tcgcgaccga gccacagtac
    24121 agcgaaaaat ttctgtacgc cgttaagcat acgcagcggc gggttcactt cgcctttttc
    24181 acgccccggc gcgcgaccaa acatcaccga tactggcacc atctgcacat ccagatttgg
    24241 gttgctacgg tgcaaatcga gatagtcgtg gaaaagctta atagactctt ctttcggcgt
    24301 gtaataggtg aatacacgcg gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc
    24361 gtcgatttcc agcggctcta acgggtcagg caaatcatgt gccagacact gggcgcgcaa
    24421 cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac ataattggac gagaggtatc
    24481 cagccccagt tccggggcag gatctgccgg aatagacttg ctttttacca ggatgcttaa
    24541 tggtaaattc agtaatttgt agtaaattcg tggccagccg gacataaacg atgtaaagcc
    24601 tctggttaat aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa attctgtggt
    24661 atccgctcat gtttcgcgcg gcgttacgca aacccgaatc atcggattta acggtacact
    24721 gatattgacg ctcataatgt aaaaaggttc tttcaatggc caataataca actggattca
    24781 cccgaattat caaagctgct ggctattcct ggaaaggttt acgcgctgca tggatcaacg
    24841 aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc
    24901 tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg
    24961 tggaaatcct caatagcgcc atcgaagcag tggttgaccg aattggctct gaataccatg
    25021 agctttccgg acgcgcaaaa gatatgggat ccgccgcggt gctgattgcc attatcgtcg
    25081 ccgtgattac ctggtgcatc ctgttatggt cgcattttgg ataacccttc cagatttcga
    25141 taaatctctg gtttattgtg cagtttatgg ttccaaaatc gccttttgct gtatatactc
    25201 acagtataac tgtatataca cccagggggc ggaatgaaag cgttaacggc caggcaacaa
    25261 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg
    25321 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg
    25381 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg
    25441 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accgcttctg
    25501 gcgcaacagc atattgaagg tcattatcag gtcgatcctt ccttgttcaa gccgaatgct
    25561 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggcgac
    25621 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt
    25681 gatgacgaag ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga actgttgcca
    25741 gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt caccattgaa
    25801 gggctggcgg ttggggttat tcgcaacggc gactggctgt aacatatctc tgagaccgcg
    25861 atgccgcttg gcgtcgcggt ttgtttttca tctctcttca tcaggcttgt ctgcatggca
    25921 ttcctcactt catctgataa agcactctgg catctcgcct tacccatgat tttctccaat
    25981 atcaccgttc cgttgctggg actggtcgat acggcggtaa ttggtcatct tgatagtccg
    26041 gtttatttgg gcggcgtggc ggttggtgca acggcgacca gctttctctt tatgctgttg
    26101 ctgtttttac gcatgagcac caccgggctg actgcgcagg cttatggtgc caaaaatcct
    26161 caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg cgttgggggc cggggcgtta
    26221 attgcgctgt tgcgtacgcc gattatcgat ctggcgctgc atattgttgg cggtagtgaa
    26281 gcggtcctgg aacaggcgcg gcgctttctt gaaatccgct ggttaagcgc tccggcgtcg
    26341 ctggcgaatc tggtattact cggttggtta ctcggcgtgc aatatgcccg tgcgccagta
    26401 attttgttag tggtcggcaa tatcctcaac attgtgctgg atgtctggct ggtgatgggg
    26461 ctgcatatga acgtgcaggg cgcggcgctg gcgacggtta ttgcggaata tgcaacattg
    26521 ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg
    26581 ctgaaaactg cctggcgagg aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg
    26641 ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca ccgtacttgg tgcgcgactg
    26701 gggagtgaca ttatcgctgt taacgcggtt ctgatgacgc tactcacctt taccgcctat
    26761 gcgcttgatg gttttgccta cgcggttgaa gcgcactccg gtcaggcata cggtgcgcgc
    26821 gacggtagcc agttgctgga tgtatggcgg gcagcgtgcc gccagtcggg gattgtagcg
    26881 ttactgtttt cggtggttta tttgctggca ggggaacaca tcattgcgtt gctgacgtcg
    26941 ttaacccaga ttcagcagct ggctgaccgc tatcttatct ggcaggtgat tttgccgttg
    27001 gttggcgtct ggtgttatct gctggacggc atgtttatag gcgcaacgcg tgccgccgaa
    27061 atgcgtaaca gtatagcggt ggccgccgca ggttttgcgc tgacgctcct tacgctgccg
    27121 tggctgggta atcatggttt gtggctggca ttaaccgtct ttctggcgtt gcgcgggctt
    27181 tctctggcgg ctatctggcg gcgtcactgg cgcaacggta cctggtttgc cgcaacgtga
    27241 cggttaaaaa ttctgaataa attatcctaa gccaaattgc tgactacact taatctcacg
    27301 ttcagaagaa aagtgaacgt actctcattc acaacctaac gatgaggtct tgattatgaa
    27361 taaagatgaa gccggcggta actggaaaca gtttaaaggt aaagtgaaag agcaatgggg
    27421 caaactgacc gatgatgata tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa
    27481 aatccaggaa cgttatggtt atcagaaaga tcaggcagaa aaagaggtcg tggattggga
    27541 aacccgcaat gaatatcgct ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac
    27601 gccctcttaa cgcggtttct ttttcacctg cacagagtga tcatgctggc actgttcagg
    27661 atgacgacac gcttccactt ccacgcatgc cgcacagagc ccatgtgctt caatcacatt
    27721 atgccgcagg gcaaacccca tttttgccgc cagcgtatgc ataatgtctt ccacgccttc
    27781 tgcacactct tctttcaccg cgccgcagcg atcgcaaata aacatggctg acgtatgcgt
    27841 gggctggtcg aacagatgac agagcacata actgttggtg gattccacct tatgcacaaa
    27901 accttgctca agcagaaaat ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc
    27961 agcttcgcgc agtaaatcaa gcagatcata agcgctgata gcgccatcct gcagactcat
    28021 caggcgcaac acttccaggc gctgtggggt caggcgcaca ttacgctgcg cgcagatttt
    28081 ttcagcctgc gctaataact cctgcgttgt ggtctttccc atcgggcacc tcaaagtggg
    28141 ggttaagaat gtccttactt taccatgttc caggaaaaac accgagaacc cttattgttg
    28201 ccgtaatgtt gattttctgt tttgtaggta aggtgttgtg ttgccttgtc gtaccattat
    28261 caacacgata ataattaata attcattttt aaataaattg cttcaagtta actcgctatt
    28321 tatttaactg ggtttaaacg catacctgta gttcagaaat tatgagcgat ttttgatagt
    28381 gaccagatgg caaaagaatt ataaggagga cacggtacga caaaaaaggt agatgaatca
    28441 tgttgaagat aatacccggt gcaactggat actttaataa aacactcaat tcaaatcaat
    28501 tcgaaaataa agatgcaacc aaagataaat tggataatag aggttcaatc aaaggtaaat
    28561 taaataatat atatggtaaa tctattgatt atgcagcgct gcgtcatcgg gatataatta
    28621 ttgcaaaaat agatttgttt attcaacgaa tcacgcataa tttatggcat gcacgtaaaa
    28681 aaatgtgttt ttaattgagc acataaatga tctcaaaatg tgggtcaata agtatactga
    28741 cgattgcact gatgaggatt taaacgatcg tgactttatt gcatcagttg ttgaccgggc
    28801 tatttttcat ttcgcgatta atagtatatg taatcctggg gataataaag atgcaatgcc
    28861 cattgaacaa tgtacttttg atgtagaaac taagaatggc cttccctcca cggttcagct
    28921 attttatgag gaatctaagg ataatgaacc tttagcgaat atacattttc aagcaatagg
    28981 ttctggtttt ttaacgtttg ttaatgcctg ccaggaacat gatgacaaca gcttaaaatt
    29041 atttgcttcg ctgttaattt cactttcata ttctagtgcc tacgcagatt tatcagaaac
    29101 agtgtatatt aatgaaaata atgagagcta cctgaaagct cagtttgaaa aattatatca
    29161 acgtgatatg aagaagtacc tgggagagat gaagcgtctg gcagatgggg gagaaatgaa
    29221 ttttgatggc tatctggata agatgtcaca tctggtgaat gaaggaacgc tcgatcctga
    29281 tattttaagc aaaatgcgag atgctgcacc acaattaatt agcttcgcga agtcgtttga
    29341 cccaacctca aaggaagaga ttaaaatact tacagacact tctaaattaa tttatgactt
    29401 gttcggggtt aaatcggaga aataatatgt gaagttcttc gatagtatgg aaggcattat
    29461 ataaaaggac ccaatattta ttgggttctt ttttctctat caatgctatt agcagggaga
    29521 tatatcacca gagtttaatg tgtgattttt tatttatcgt cgaacctgga ttgtttatca
    29581 ttggccttaa caaagttaac ggctaataag attatttcca tcacttcgtg agagcttcat
    29641 gccttgagag gatctcaatt ttcttttgca atgagacagg cgcttcctgt tgttatggta
    29701 tagtaccccg ctattgagcc tcctgaacag tgaagctgaa taacataacc ccatgatata
    29761 tcgataaaat aatctctaca tttgaaaatg cacggtaatt ctgaaatgca aaaaatcaac
    29821 caaaccagcg caatgcctga aaaaactgac gttcactgga gtggtcggtt tagtgttgca
    29881 ccaatgctcg actggacgga cagacattgc cgctatttct tgcgtctgct ttcccgcaat
    29941 acgttgcttt ataccgaaat ggtgaccaca ggggcgatta ttcacggtaa aggtgattat
    30001 ctggcgtaca gtgaagaaga acatccggta gcgttgcaac tgggcggtag cgatccggcg
    30061 gcgctggcgc agtgtgcaaa gctggcagaa gcgcgcggat atgatgagat caacctgaat
    30121 gtcggctgcc cgtctgaccg ggtgcagaac ggcatgtttg gtgcgtgtct gatgggtaat
    30181 gcgcagctgg ttgccgactg cgtgaaagcg atgcgcgatg tggtgtcgat tccggtgacg
    30241 gtgaaaacgc gtattggcat cgacgaccag gacagctatg aatttctctg cgatttcatc
    30301 aataccgttt ccggcaaagg cgagtgtgag atgttcatca tccacgcacg taaagcctgg
    30361 ctttcggggt taagcccgaa agaaaaccgt gaaatcccgc cgctcgatta tccgcgtgtg
    30421 tatcaactga agcgtgactt cccgcatctg acaatgtcga ttaacggtgg tatcaagtcg
    30481 ctggaagaag ctaaagcgca tttgcaacat atggatggcg tgatggtcgg gcgcgaggcg
    30541 tatcagaatc cgggtattct ggcggcggta gaccgggaga tctttggttc ctcggatacc
    30601 gatgccgatc cggtggcagt agtgcgtgcc atgtatccgt acattgagca tgaactcagc
    30661 caggggacgt atctcggcca tattatccgg catatgctgg gtttgttcca gggtattcct
    30721 ggcgcgcggc agtggcggcg ttatttaagt gaaaatgccc ataaagcggg tgctgacatt
    30781 aacgtgctgg aacacgcgct caaactggtg gcggataagc gttaactttt caccaaaaag
    30841 tagtcaaatt caccacgccc tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga
    30901 ttatttttgg catgattctt gtaatgccag caagagattt catatttggg agagcatcat
    30961 gctggaacta ctttttgtga ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt
    31021 gctgggcatt atcgccgcgc tggttgtggc gacggccatt atgttcctcg gcggtatgct
    31081 ggcattgatg attaagttgc tgccgtggtt actactggcg attgcggtgg tgtgggttat
    31141 taaggcgatt aaagcaccaa aaatgccgaa atatcagcgt tatgaccgct ggcgttacta
    31201 agggattgtg cggatgatca caacctaagg ttttatcctt agaacaaaat aggaattgat
    31261 aatcaaatct gtcactattg cgcctctaac agattcatcg tgctgtaccc tacatacagc
    31321 cgaactataa aaagaaaggg cttcccaggt ggaagcccta tttcttttat ggaatcagca
    31381 ggctggaacc ttgcgtcgcc cggctttcca gaatctcatg cgcacgctgc gcatccttca
    31441 gcggatattt ctgctgctcg gcgacatcga ccttaatcac accgctggca atcaaagaga
    31501 acagttcatt actggcctcg gttaattcct cccgcgtggt gatatagcct tgcagggaag
    31561 ggcgtgtcac atacaacgag cctttttgat tgagaatgcc taagttcaca ccggtaaccg
    31621 cacctgatga gttgccaaaa ctgaccatta agccgcggcg ttgcaggcaa tccagcgacc
    31681 gttcccaggt gtctctgccc acggaatcgt acactacgcg cactttctta ccgccggtga
    31741 tctctttcaa tcgctcgacc agattctctt cacgatagtt aataacctgc cacgcgccgg
    31801 cttttagcgc gctctgcgct ttttgcgcgg ttcctacggt gccgatgagt ttcgcgccca
    31861 gggcttttgc ccactggcag gcaattaagc caatgccgcc agccgctgcg tggaacagga
    31921 actgttcatc gggtttaatt tcataggttt tgcgcagcag ataataaacc gttaagcctt
    31981 tcaggaagga tgccgcagct tgctcaaaag aaattgccgc aggcagaatc gccgctttat
    32041 ccgcattaat gttatgcaca gagctgtaag cgcctaacgc cgactgcgca tagactacat
    32101 gatcgccagc cttaatatgc tttacaccac tgccgacttt actcacgatg cctgccgctt
    32161 cggtacctaa tccgctgggt agcgatggcg gcgggtaaag gccgctgcgg atatacgtgt
    32221 cgataaaatt gatgccaatg gctttatttt cgacctggat ttcattctcc gccggatcgg
    32281 caggagtgaa ctctacggct tgaagtactt ccgggccacc gtgcttgtga aattcaattc
    32341 gtgttgccat gtgtcctcca gaacgtatcg tcagggtctg cttcatatgg taaagtttcg
    32401 acccattctt tatttcggta actccattca ctatggcagg aaataaaccc ttcaacaaac
    32461 agcaggctga accccgcgaa cgcgatccac aagttgccgg gctgaaagtg cctccgcact
    32521 cgatcgaagc ggagcagtcg gtgttgggcg gtttaatgct ggataacgaa cgctgggatg
    32581 atgtagccga gcgtgtggta gcagacgatt tttacacccg cccacaccgt catatcttta
    32641 ctgaaatggc gcgtttgcag gaaagcggta gtcctatcga tctgattacc cttgcggaat
    32701 cgttggaacg ccaggggcaa ctcgatagcg tcggtggttt tgcttatctg gcagagctgt
    32761 caaaaaatac gccaagtgcg gcgaacatca gtgcttatgc ggacatcgtg cgtgaacgtg
    32821 ccgttgtccg tgagatgatc tcggttgcga atgagattgc tgaagccggt tttgatccgc
    32881 aggggcgtac cagcgaagat ctgctggacc ttgctgaatc ccgcgtcttt aaaattgccg
    32941 aaagtcgtgc aaacaaagac gaagggccga agaacatcgc cgatgtgctc gacgcaaccg
    33001 tggcgcgtat tgagcagttg tttcagcagc cacacgatgg cgttaccggg gtaaacaccg
    33061 gttatgacga tctcaacaaa aaaaccgctg gcttgcagcc gtcggatttg atcatcgtcg
    33121 ccgcgcgtcc gtcgatgggt aaaacaacat ttgcgatgaa cctcgtcgaa aacgcggcaa
    33181 tgttgcagga taaaccagta cttatcttct cgctggagat gccatcagaa cagatcatga
    33241 tgcgttctct ggcgtcgctg tcgcgcgttg accagactaa aatccgtacc gggcagctcg
    33301 atgatgaaga ctgggcgcgc atttccggca ccatgggtat tttgctcgaa aaacgcaata
    33361 tctatatcga tgactcttcc ggcctgacgc caacggaagt gcgttcccgc gcacgccgta
    33421 ttgcccgtga acacggcggc atcgggctta tcatgatcga ctacctgcaa ctgatgcgcg
    33481 taccggcgct ttccgataac cgtacgctgg aaattgcaga aatctcccgc tcgctgaaag
    33541 cactggcgaa agaactgaac gtgccggtgg tggcgctgtc ccagttgaac cgttctctgg
    33601 aacaacgtgc cgacaaacgt ccggtcaact ccgacctgcg tgaatctggc tctatcgagc
    33661 aggatgcgga cttgatcatg tttatctatc gtgatgaggt gtatcacgaa aacagtgatt
    33721 taaaaggcat cgcggaaatt attatcggta aacaacgtaa cggcccaatc gggacggtac
    33781 gcctgacctt taacggtcaa tggtcgcgct tcgacaacta tgcggggccg cagtacgacg
    33841 acgaataata attattttat gaattaggta attaaagcaa acaattatca aggaacacaa
    33901 atgcaagcgg caactgttgt gattaaccgc cgcgctctgc gacacaacct gcaacgtctt
    33961 cgtgaactgg cacctgccag taaaatggtt gcggtggtga aagcgaacgc ttatggtcac
    34021 ggtcttcttg agaccgcgcg aacgctcccc gatgctgacg cctttggcgt agcccgtctc
    34081 gaagaagctc tgcgactgcg tgcgggggga atcaccaaac ctgtactgtt actcgaaggc
    34141 ttttttgatg ccagagatct gccgacgatt tctgcgcaac attttcatac cgccgtgcat
    34201 aacgaagaac agctggctgc gctggaagag gctagcttgg acgagccggt taccgtctgg
    34261 atgaaactcg ataccggtat gcaccgtctg ggcgtaaggc cggaacaggc tgaggcgttt
    34321 tatcatcgcc tgacccagtg caaaaacgtt cgtcagccgg tgaatatcgt cagccatttt
    34381 gcgcgcgcgg atgaaccaaa atgtggcgca accgagaaac aactcgctat ctttaatacc
    34441 ttttgcgaag gcaaacctgg tcaacgttcc attgccgcgt cgggtggcat tctgctgtgg
    34501 ccacagtcgc attttgactg ggtgcgcccg ggcatcattc tttatggcgt ctcgccgctg
    34561 gaagatcgct ccaccggtgc cgattttggc tgtcagccag tgatgtcact aacctccagc
    34621 ctgattgccg tgcgtgagca taaagccgga gagcctgttg gttatggtgg aacctgggta
    34681 agcgaacgtg atacccgtct tggcgtagtc gcgatgggct atggcgatgg ttatccgcgc
    34741 gccgcgccgt ccggtacgcc agtgctggtg aacggtcgcg aagtaccgat tgtcgggcgc
    34801 gtggcgatgg atatgatctg cgtagactta ggtccacagg cgcaggacaa agccggggat
    34861 ccggtcattt tatggggcga aggtttgccc gtagaacgta tcgctgaaat gacgaaagta
    34921 agcgcttacg aacttattac gcgcctgact tcaagggtcg cgatgaaata cgtggattaa
    34981 tcgttctgta atatttgatt gtctgtgccg gatgcggcgt gaatgcctta tccggccaat
    35041 aaaatcctaa aaattcaata agttgatgtt ctttcatgct cttataaagg tcgtgcctct
    35101 ggcggatgta cgtttatcat tagtctcact ctgttgctaa ttgccgttcg ctcctgaaca
    35161 tccactcgat cttcgccttc ttccggttta ttgtgtttta accacctgcc cgtaaacctg
    35221 gagaaccatc gcgtgtttca aaaagttgac gcctacgctg gcgacccgat tcttacgctt
    35281 atggagcgtt ttaaagaaga ccctcgcagc gacaaagtga atttaagtat cggtctgtac
    35341 tacaacgaag acggaattat tccacaattg aaagccgtgg cggaggcgga agcgcgcctg
    35401 aatgcgcagc cccatggcgc ttcgctttat ttaccgatgg aagggcttaa cagctatcgc
    35461 catgccattg cgccgctgct gtttggtgcg gaccatccgg tactgcaaca acagcgcgta
    35521 gcaaccattc aaacccttgg cggctcaggg gcattgaaag tgggcgcgga tttcctgaaa
    35581 cgctacttcc cggaatcagg cgtctgggtc agcgatccta cttgggaaaa ccacgtagca
    35641 atattcgccg gggctggatt cgaagtgagt acttacccct ggtatgacga agtgactaac
    35701 agcgtgcgct ttaatgacct gttggcgacg ctgaaaacat tacctgcccg cagtattgtg
    35761 ttgctgcatc catgttgcca caacccaacg ggtgccgatc tcactaatga tcagtgggat
    35821 gcggtgattg aaattctcaa agcccgcgag cttatcccat tcctcgatat tgcctatcaa
    35881 ggatttagtg ccggtatgga agaggatgcc tacgctattc gcgccattgc cagcgctgga
    35941 ttacccgctc tggtgagcaa ttcgttctcg aaaattttct ccctttacgg cgagcgcgtc
    36001 ggcggacttt ctgttctgtg tgaagatgcc gaagccgctg gccgcgtact ggggcaattg
    36061 aaagcaacag ttcgccgcaa ctactccagc ccgccgaatt ttggtgcgca ggtggtggct
    36121 gcagtgctga atgacgaggc attgaaagcc agctggctgg cggaagtaga agagatgcgt
    36181 actcgcattc tggcaatgcg tcaggaattg gtgaaggtat taagcacaga gatgccagaa
    36241 cgcaatttcg attatctgct taatcagcgc ggcatgttca gttataccgg tttaagtgcc
    36301 gctcaggttg accgactacg tgaagaattt ggtgtctatc tcatcgccag cggtcgcatg
    36361 tgtgtcgccg ggttaaatac ggcaaatgta caacgtgtgg caaaggcgtt tgctgcggtg
    36421 atgtaatgca ggaaagcagg ctggagttac ccagcctgca gtgaaattaa actgtcgtcg
    36481 ctttcactat ttctttgtag atgatttttt tgatgccgtt gttctacgtg agagataata
    36541 aaacgttgtc agttctttta ttgttaagtt tatcccaatt atctggaatt cctttcccta
    36601 ttttttcgtg tggagtaata at
//
LOCUS       DAECOD010000029        36520 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.029, whole genome shotgun sequence.
ACCESSION   DAECOD010000029 DAECOD010000000
VERSION     DAECOD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.029"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(156..1391)
                     /locus_tag="KKO15_001520"
     CDS             complement(156..1391)
                     /locus_tag="KKO15_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD6360357.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KKO15_001521"
     tRNA            complement(1538..1614)
                     /locus_tag="KKO15_001521"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KKO15_001522"
     tRNA            complement(1657..1743)
                     /locus_tag="KKO15_001522"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KKO15_001523"
     tRNA            complement(1764..1839)
                     /locus_tag="KKO15_001523"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KKO15_001524"
     tRNA            complement(1898..1974)
                     /locus_tag="KKO15_001524"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KKO15_001525"
     CDS             complement(2077..3462)
                     /locus_tag="KKO15_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6360358.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KKO15_001526"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KKO15_001526"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBD6360359.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KKO15_001527"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KKO15_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBD6360360.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KKO15_001528"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KKO15_001528"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBD6360361.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KKO15_001529"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KKO15_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBD6360362.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KKO15_001530"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KKO15_001530"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBD6360363.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KKO15_001531"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KKO15_001531"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBD6360364.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KKO15_001532"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KKO15_001532"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD6360365.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KKO15_001533"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KKO15_001533"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD6360366.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KKO15_001534"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KKO15_001534"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBD6360367.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KKO15_001535"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KKO15_001535"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBD6360368.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KKO15_001536"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KKO15_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBD6360369.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KKO15_001537"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KKO15_001537"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBD6360370.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KKO15_001538"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KKO15_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBD6360371.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KKO15_001539"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KKO15_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBD6360372.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KKO15_001540"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KKO15_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBD6360373.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KKO15_001541"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KKO15_001541"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBD6360374.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KKO15_001542"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KKO15_001542"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBD6360375.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KKO15_001543"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KKO15_001543"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBD6360376.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KKO15_001544"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KKO15_001544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBD6360377.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KKO15_001545"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KKO15_001545"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBD6360378.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KKO15_001546"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KKO15_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBD6360379.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KKO15_001547"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KKO15_001547"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBD6360380.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KKO15_001548"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KKO15_001548"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD6360381.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KKO15_001549"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KKO15_001549"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBD6360382.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KKO15_001550"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KKO15_001550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBD6360383.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KKO15_001551"
     CDS             complement(31267..32913)
                     /locus_tag="KKO15_001551"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBD6360384.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KKO15_001552"
     CDS             complement(32916..32966)
                     /locus_tag="KKO15_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360385.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KKO15_001553"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KKO15_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBD6360386.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KKO15_001554"
     CDS             33502..35022
                     /locus_tag="KKO15_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBD6360387.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KKO15_001555"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KKO15_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBD6360388.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KKO15_001556"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KKO15_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBD6360389.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KKO15_001557"
     tRNA            complement(36439..36514)
                     /locus_tag="KKO15_001557"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9998 c         9201 g         8403 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtatat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAECOD010000030        36238 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.030, whole genome shotgun sequence.
ACCESSION   DAECOD010000030 DAECOD010000000
VERSION     DAECOD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.030"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            151..675
                     /locus_tag="KKO15_001558"
     CDS             151..675
                     /locus_tag="KKO15_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6360390.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            756..1430
                     /locus_tag="KKO15_001559"
     CDS             756..1430
                     /locus_tag="KKO15_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD6360391.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1446..3926
                     /locus_tag="KKO15_001560"
     CDS             1446..3926
                     /locus_tag="KKO15_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6360392.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3942..4975
                     /locus_tag="KKO15_001561"
                     /pseudo
     CDS             3942..4975
                     /locus_tag="KKO15_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5057..5395)
                     /gene="rcnB"
                     /locus_tag="KKO15_001562"
     CDS             complement(5057..5395)
                     /gene="rcnB"
                     /locus_tag="KKO15_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBD6360393.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5614..6453)
                     /gene="rcnA"
                     /locus_tag="KKO15_001563"
                     /pseudo
     CDS             complement(5614..6453)
                     /gene="rcnA"
                     /locus_tag="KKO15_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6575..6847
                     /gene="rcnR"
                     /locus_tag="KKO15_001564"
                     /pseudo
     CDS             6575..6847
                     /gene="rcnR"
                     /locus_tag="KKO15_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      6939..7035
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7070..7858
                     /gene="thiM"
                     /locus_tag="KKO15_001565"
     CDS             7070..7858
                     /gene="thiM"
                     /locus_tag="KKO15_001565"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBD6360394.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7855..8655
                     /gene="thiD"
                     /locus_tag="KKO15_001566"
     CDS             7855..8655
                     /gene="thiD"
                     /locus_tag="KKO15_001566"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBD6360395.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8720..9541
                     /locus_tag="KKO15_001567"
     CDS             8720..9541
                     /locus_tag="KKO15_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD6360396.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9589..10335
                     /locus_tag="KKO15_001568"
     CDS             9589..10335
                     /locus_tag="KKO15_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6360397.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10309..11274)
                     /locus_tag="KKO15_001569"
     CDS             complement(10309..11274)
                     /locus_tag="KKO15_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBD6360398.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11271..12274)
                     /locus_tag="KKO15_001570"
                     /pseudo
     CDS             complement(11271..12274)
                     /locus_tag="KKO15_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(12271..13548)
                     /locus_tag="KKO15_001571"
     CDS             complement(12271..13548)
                     /locus_tag="KKO15_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6360399.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13805..14857
                     /gene="fbaB"
                     /locus_tag="KKO15_001572"
     CDS             13805..14857
                     /gene="fbaB"
                     /locus_tag="KKO15_001572"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBD6360400.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15165..16019
                     /gene="gatY"
                     /locus_tag="KKO15_001573"
     CDS             15165..16019
                     /gene="gatY"
                     /locus_tag="KKO15_001573"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBD6360401.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            16048..17310
                     /gene="gatZ"
                     /locus_tag="KKO15_001574"
     CDS             16048..17310
                     /gene="gatZ"
                     /locus_tag="KKO15_001574"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBD6360402.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            17320..17772
                     /gene="gatA"
                     /locus_tag="KKO15_001575"
     CDS             17320..17772
                     /gene="gatA"
                     /locus_tag="KKO15_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD6360403.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17803..18087
                     /gene="gatB"
                     /locus_tag="KKO15_001576"
     CDS             17803..18087
                     /gene="gatB"
                     /locus_tag="KKO15_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBD6360404.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            18091..19446
                     /locus_tag="KKO15_001577"
     CDS             18091..19446
                     /locus_tag="KKO15_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBD6360405.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            19494..20534
                     /gene="gatD"
                     /locus_tag="KKO15_001578"
     CDS             19494..20534
                     /gene="gatD"
                     /locus_tag="KKO15_001578"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD6360406.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            20640..21413
                     /locus_tag="KKO15_001579"
     CDS             20640..21413
                     /locus_tag="KKO15_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6360407.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21495..22394)
                     /gene="yegS"
                     /locus_tag="KKO15_001580"
     CDS             complement(21495..22394)
                     /gene="yegS"
                     /locus_tag="KKO15_001580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBD6360408.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            22800..23117
                     /locus_tag="KKO15_001581"
     CDS             22800..23117
                     /locus_tag="KKO15_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360409.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(23459..24820)
                     /gene="yegQ"
                     /locus_tag="KKO15_001582"
     CDS             complement(23459..24820)
                     /gene="yegQ"
                     /locus_tag="KKO15_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBD6360410.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24967..25298)
                     /locus_tag="KKO15_001583"
                     /pseudo
     CDS             complement(24967..25298)
                     /locus_tag="KKO15_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(25489..26211)
                     /gene="baeR"
                     /locus_tag="KKO15_001584"
     CDS             complement(25489..26211)
                     /gene="baeR"
                     /locus_tag="KKO15_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBD6360411.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(26208..27611)
                     /gene="baeS"
                     /locus_tag="KKO15_001585"
     CDS             complement(26208..27611)
                     /gene="baeS"
                     /locus_tag="KKO15_001585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBD6360412.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(27608..29023)
                     /locus_tag="KKO15_001586"
     CDS             complement(27608..29023)
                     /locus_tag="KKO15_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6360413.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(29024..32089)
                     /gene="mdtC"
                     /locus_tag="KKO15_001587"
     CDS             complement(29024..32089)
                     /gene="mdtC"
                     /locus_tag="KKO15_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBD6360414.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(32090..35212)
                     /gene="mdtB"
                     /locus_tag="KKO15_001588"
                     /pseudo
     CDS             complement(32090..35212)
                     /gene="mdtB"
                     /locus_tag="KKO15_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(35212..>36238)
                     /gene="mdtA"
                     /locus_tag="KKO15_001589"
     CDS             complement(35212..>36238)
                     /gene="mdtA"
                     /locus_tag="KKO15_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBD6360415.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         9155 a         8892 c         9407 g         8784 t
ORIGIN      
        1 atttgttgta tttatattgc attggttaat ttgttttaaa tcaataaaac aatatgttta
       61 cattgcggca gcttttttga tgtgagaaat ttgtttccaa gagatacaga ctcttaacaa
      121 ccaaaaataa attttattaa ggaacatatt atgaaacgtt caattattgc tgccgctgtc
      181 ttttcttctt tttttatgag cgctggagta tttgctgcag acgttgatac cggaacatta
      241 accattaaag gaaatattgc agaatctccg tgtaaattcg aagcgggtgg tgattcagta
      301 agtattaata tgccgactgt accaaccact gtctttgaag gtaaagctaa atattctacc
      361 tatgatgatg cagtcggtgt aaccagcagc atgttaaaaa ttagctgccc gaaagaagtt
      421 gctggtgtaa aactctcgtt gatcacaaac gacaaaataa ccggtaacga taaggcgata
      481 gccagtagca acgataccgt aggtgataac agcgatgtcc tagatgtttc tgcacctttt
      541 aacattgaga gttataaaac agcggaaggt caatatgcta ttccgtttaa agcaaaatac
      601 ctgaaactga cagataactc agtgcaatca ggtgatgtgt tatcttctct ggttatgcgt
      661 gtggcgcagg attaataaat aaaattgcaa gtttctccag gtaataaaca tggccgctat
      721 ccccatggcg gctttttaat ttaagaggca ttaagatgaa aggattatta tctttactca
      781 ttttttctat ggtccttcct gcacatgccg gaattgttat ctacgggacg cgcattattt
      841 acccggcaga aaataaagaa gttatggtgc agttgatgaa ccagggaaat cgttcttcgc
      901 tgctgcaggc gtggattgat gatggcgata cgtcgttacc accagaaaaa attcaggttc
      961 ctttcatgtt aacgccacca gtggcaaaaa taggggcaaa ttccgggcag caagtaaaaa
     1021 tcaaaattat gccgaataaa ctgcccacta ataaagaaag cattttttat ctgaatgttc
     1081 tgtatattcc accaaatagt ccagagcaag aaggcaagaa tgcactgaag tttgcgatgc
     1141 aaaacagaat taagttgttt taccggccag cgggtattgc tccggtaaat aaagcgacct
     1201 ttaaaaagtt actggtaaat cgcagtggca atggattggt gataaaaaat gactcagcta
     1261 attgggtgac gatttcggat gtcaaagcta ataatgttaa agtcaattat gaaactatta
     1321 tgattgcccc cttagaaagt cagagtgtta atatcaaaag taataatgca aataactggt
     1381 atctgaccat tatcaatgac cacggcaact atattagtga caaaatttaa ttgcaggagc
     1441 tgcctatgtt gagaatgacc ccacttgcat cagcaatcgt agcgttattg ctcggcattg
     1501 aagcttatgc agctgaagaa acctttgata cccattttat gataggtgga atgaaagacc
     1561 agcaggttgc aaatattcgt cttgatgata atcaaccctt accggggcag tatgacatcg
     1621 atatttatgt caataagcaa tggcgcggga aatatgagat tattgttaaa gacaacccgc
     1681 aagaaacatg tttatcaaga gaagttatca agcggttagg cattaatagc gataacttcg
     1741 ccagcggtaa gcaatgttta acatttgagc aacttgttca gggtgggagc tatagctggg
     1801 atatcggggt ttttcgtctc gatttcagtg tcccgcaggc ttgggtggaa gaactggaaa
     1861 gtggctatgt tccaccggaa aactgggagc ggggtattaa tgcgttttat acttcttatt
     1921 atgtgagtca gtattacagc gactataaag cgtcgggtaa caacaagagt acatatgtac
     1981 gttttaacag cgggttaaat ttactggagt ggcaactgca ttctgatgcc agtttcagta
     2041 aaacaaataa caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg
     2101 cccaatttct cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt
     2161 ctgttcgctt cagcggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac
     2221 aaaattttac gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac
     2281 agaatggttt tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt
     2341 tgcagttggc cggtggtgga gcagatcttg atgttagcgt gaaagaggcg gacggctcgg
     2401 taaccaccta tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga
     2461 aatatgattt tgcggcgggc cgtagccata ttgaaggggc gagcaagcaa agtgattttg
     2521 tccaggcagg ttatcagtat ggttttaata acttattgac gctgtatggc ggcacgatgg
     2581 ttgctaataa ttactatgcg tttaccctcg gaacgggttg gaacacgcgc attggcgcaa
     2641 tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc
     2701 aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg
     2761 cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca
     2821 ataaagataa ttatcgccgt gatgaaaacg atatctatga cattgccgat tattaccaga
     2881 acgattttgg ccgtaaaaat agcttttctg ccaatatgag ccagtcattg ccagaaggct
     2941 ggggttctgt gtccttaagt acgttatggc gagattactg ggggcgtagc ggcagcagta
     3001 aggattatca gttgagttat tccaataact ggcggcggat aagctatacc ctcgcggcaa
     3061 gccaggcgta tggcgagaat catcatgaag agaaacgttt taatattttt atatcaattc
     3121 cctgtgattg gggtgatgac gttacgacgc ctcgtcggca aatatatatg tctaactcaa
     3181 cgacgtttga tgatcagggt tttgcctcaa ataatacggg attatcagga acagtaggga
     3241 gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga
     3301 cagctggggc gaatttgacc tggaacgcgc cggttgcgac agtgaatggc agttatagtc
     3361 agtcgagtac ttatcgacag actggagcca gtgtttcagg gggcattgtc gcctggtcgg
     3421 gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa
     3481 ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg
     3541 tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg
     3601 atagcgaagc agaattacgt ggcaaccgga aaattgccgc cccttatcgc ggcgcggttg
     3661 tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg
     3721 atggtcaacc attaacgttt ggttatgaag tcaatgatat ccatggtcat aatattggcg
     3781 ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg
     3841 tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg
     3901 aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc
     3961 tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat
     4021 ctgttcagca caatttggtt gttcagggga attttgcact tactcaaaca caaatagcga
     4081 catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga
     4141 gcccatcgga tattattgtt ggactttata acgataccat taaattaaat ttacattttg
     4201 aatggaccaa taaaaacaac atcacgttgt caaataatca gagcagtttc accagtggtt
     4261 attcggtaac ggtgacacct gcggccagta atgcaaaagt gaatatttct gcggggggcg
     4321 gtggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg
     4381 ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg ggtgcatgtg
     4441 taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttc aatctgacat
     4501 tgtcatacaa cccgataacc acaacctgca aacaggacga tttattaatt actttagaca
     4561 gtattcccgt ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaaaaaa
     4621 gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa
     4681 tgcaggtgta tttatcaagt tctgacttgt taaccaacag caacacaata ctgaaaggtg
     4741 cggaagataa tgcgtaggat ttattcttga aagtaatggt tcgccagtca cacttttaaa
     4801 tatcactaac agcagtaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     4861 tacagataca acggtttcaa ttccgataac agccagttac tacgtctacg atacaaacaa
     4921 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     4981 agcaaaacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     5041 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     5101 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     5161 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     5221 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     5281 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     5341 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     5401 ctccactttg gggatctgta atatcagtcg catatctttc ctgtcataca atataactga
     5461 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     5521 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     5581 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     5641 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     5701 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     5761 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     5821 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     5881 ggggataaag ccaccggtta agccaaataa caaaatttgc cagttgatga cctctctacc
     5941 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     6001 atgttcgtga tggtgatgat gtccttggtc gtggtgatgt tcgtggtctt gatgatgttc
     6061 gtgatcgtga tgatgctcat aatcatgccc gtgcatattc tccagccagt tgcgttcgcc
     6121 gcgctaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg cggaaatcag
     6181 ctggagccac ggttctgctg attgagcagt aaagcgctta ctgatcacca tcccgccaaa
     6241 ggcaatcaac cagaccaccg cggtatgcga aatggttgct gccagtccga gcatcaccgc
     6301 ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt ttgagtgccc
     6361 tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga accaggcgtt
     6421 tccttgctga agaagagttg taaattcggt catgataata attcttagta ttaattcggc
     6481 aatctgattc tactcccccc cagtacctga tactaccccc ccagtagatt aatagtgcta
     6541 tgatttttca tattcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca
     6601 gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct
     6661 cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggagcggt
     6721 aaacggtctg atgcgggaag tgattaaagg tcatctgacg gaacacatcg ttcactaggg
     6781 ggatgagcta aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg attcatatat
     6841 caaataattt attaacgcga ttgtaaaact gccgtttttc ctcgtttaca acgggtgcgc
     6901 tggacattac catcctcctc tgcgatttat catcgcaacc aaacgactcg gggtgccctt
     6961 ctgcgtgaag gctgagaaat acccgtatca cctgatctgg ataatgccag cgtagggaag
     7021 tcacggacca ccaggtcatt gcttcttcac gttatggcag gagcaaacta tgcaagtcga
     7081 cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac attcccctct
     7141 tgtgcactgc atgaccaatg atgtggtgca aacctttacc gccaatacct tgctggcgct
     7201 cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg cggctatcgc
     7261 cagtgccttg ttgattaacg ttggcacact gacgcagcca cgcgcacagg cgatgcgtgc
     7321 tgccgttgag caagcaaaaa gctctcaaac acgctggacg cttgatccag tagcggttgg
     7381 tgcgctcgat tatcgccgcc atttttgtca tgaactttta tcttttaaac cggcagcgat
     7441 acgtggtaat gcttcggaaa tcatggcatt agctggcatt gctaatggcg gacggggagt
     7501 ggataccact gacgccgcag ctaacgcgat acccgctgca caaacactgg cacgggaaac
     7561 tggcgcaatc gtcgtggtca ctggcgaggt ggattatgtt accgatggac atcgtatcat
     7621 tggtattcac ggtggcgatc cgttaatgac caaagtggta ggaactggct gtgcattatc
     7681 ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg ctggaaaatg tcgcatctgc
     7741 ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc agaagcgagg ggccaggcag
     7801 ttttgttcca catttccttg atgcgctctg gcaattgacg caggaggtgc aggcatgaaa
     7861 cgaattaacg ctctgacgat tgccggtact gatccgagtg gtggtgcggg gattcaggcc
     7921 gatcttaaaa ccttctcggc acttggcgct tatggttgct cagttattac tgcactggtg
     7981 gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg agcctgattt tgtcgccgcc
     8041 cagctcgatt cggtgttcag cgatgtgcga atcgatacca ctaaaatcgg tatgttggcg
     8101 gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac gttatcagat ccaaaacgtg
     8161 gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc tgctttcacc ttcggcggtt
     8221 gctacgctgc gcagtcgatt attgccacag gtttcattaa taacgccaaa cttgcccgaa
     8281 gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac aggaaatgct ggaacaaggg
     8341 cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga aaggtggtca tctggatgat
     8401 gagcaaagcc cggactggct gtttacccgc gagggtgaac aacggtttac cgcaccgcgc
     8461 attatgacca aaaacaccca cggcactggt tgtacactct ctgcggcgtt ggctgcacta
     8521 cgcccgcgcc atacaaactg ggctgacacc gtacaggagg caaaaatctg gctttcatcg
     8581 gcgttagccc aggccgacac gctggaagtt ggtcacggta ttggtccggt tcaccacttc
     8641 cacgcctggt ggtgagcacc gtactatagc tgctttggtt attgtcagcc tttggcactg
     8701 acattcaggg atgaaaccaa tgcagctaag aattaccagt cgaaaaaaac tcaccgctct
     8761 attatgcgct cttgggctaa tttccatcgt tgcaatttat ccgcgtcaga cggtgaattt
     8821 tttctattcg acagcagttc agattacaga ctacatccac ttctacggtt atcgcccggt
     8881 taaatctttc gctattcgca ttcctgccag ttacaccatt cacggattag atgtttcgcg
     8941 ctggcaggag cgtatcgact ggcagcgggt ggcaaaaatg cgcgacaacg atatccgctt
     9001 acagtttgct tttattaagg ctacggaagg cgaaaagctg gtggacccct atttttcgcg
     9061 taactggcaa ttaagccgcg aaaatggcct gctgcgcggg gcgtatcatt atttctcccc
     9121 gtcggtatcc gcttcagttc aggcgcgatt atttctgcaa acggtggatt tctcacaagg
     9181 cgatttccct gccgtgctgg acgtagaaga acggggaaaa ttatcggcaa aagaattacg
     9241 caagcgggta agtcagtggc taaaaatggt cgaaaaacgc acgggaaaaa agccgattat
     9301 ttactcagga gccgtttttt atcacaccaa tctggcgggc tatttcaatg aatatccgtg
     9361 gtgggtggct cactattatc aacgtcgtcc ggacaatgac ggcatggcct ggcgcttctg
     9421 gcagcattcc gaccgtggac aggtagatgg catcaatggt ccggtggatt ttaatgtgtt
     9481 taatggcacg gggatgagtt gcaggcattc gttgatggga ttaaagaaac gccttaaata
     9541 agaagcagtg atatactccc accagactcc ctggcaggaa aaagacaaat ggaacaagcg
     9601 catacccagc tgattgccca actgaacgag cgtattttag cggcggataa cacgccgctt
     9661 tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca gcggggtgct ggagcatggc
     9721 aatattttgc ccggtgagcg tgatttaagt cagttaaccg gcgtttcgcg cattacggtg
     9781 cgcaaggcga tgcaggcgct ggaagaagag ggcgtggtga cgcgttcgcg cggttacggc
     9841 acgcagatca acaacatctt cgaatactcg ttgaaagaag cgagaggttt ttctcagcag
     9901 gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta ataagcgtgt cgtgaaatgt
     9961 cccgaagaag ttgcgcagca gctggcggtc gaagcgggaa gtgatgtctt tttgcttaag
    10021 cgtattcgct atgtcgatga agaagcggta tcgattgagg aatcgtgggt tccggcgcat
    10081 ttaattcatg atgttgatgc catcggaatt tctctttatg actatttccg cagccagcac
    10141 atttacccac aacgtacgcg ttcccgcgtt agcgcccgga tgccggatgc cgagtttcag
    10201 tcacatattc agttagatag caaaataccg gtgctggtga tcaagcaagt ggcgcttgac
    10261 caacagcaac ggcctattga gtacagcatt agccactgtc gcagcgatct atacgttttt
    10321 gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca ccgccccgat gcccgacaac
    10381 ccacgacgcc actgcattgc ccagtaatac ggcatccgcc agtggcagac cagaggccag
    10441 cccggcaagt acgccacctg catgactgtc gcccgcccca atggtgtcta caacttgcgt
    10501 tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa taccatgcgc cttctttatc
    10561 gagacgaacg atcaacggcg cggcaaattt ctcctgccat tgcttgccaa gtgttgttat
    10621 ctctgcggat aaagcaaaac gttcggcggc aatctcagcc tcttgacgat tgagtgacac
    10681 taaaggtcga caggccatga tccgcgccag taatgcgtcc gggatatcgc caatacgtgg
    10741 gcaaaaatcg ataaacggcg tcacgtcttg cagcttttcc agccattcca ctaacaattc
    10801 gccgcagggc gaggccagtt gataaccgga aaaatagagc aggttgccag gcgcaacggt
    10861 taatcgtgcc aaccattggc gattccattg attttccaca ccgctgaatg acataaaagt
    10921 gcgttcgcca tccggctcaa ccagtgccag acaccagccg ttatcgcttt cggcgttatc
    10981 gatcaggctg attaacccct ctttcgccat ccggttgcga ataatctccg cccacacgcc
    11041 ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg cgctttaacg ccacggcaat
    11101 attcagtgcg cagccgccaa cgttaacgct ctgctgtttc agttcgatat cgcacccacg
    11161 ccaggggagg gcataagcgt cggcgatcac gtcaatgacc gccgcgccga cgaccatcac
    11221 cggctgacgc gtggttaatt caggcagcag tgtgtgtaat cgagcgccgc tcataccgcc
    11281 tcccgttgtt gacgatattt cgccagcgct gtggcatagc ggttgaaatc aagctgattt
    11341 accgcatcca gctccgcctt taacgcagga tcgacagcgt taatgccatg caacgcgccg
    11401 caaatcgccg tcgccatagc acctatggtg tctgtgtcgc cgccaagatt ggcgcacagg
    11461 acggcgcagc ggtttgggtc ggtttgtgcc agttcaacta gcgcaatggc gcacggaacg
    11521 gactcaatag tgctggtgcc tgcgccaacg acctggtaaa gctgttcgct ggcggattcg
    11581 gtgccgtcgg cattacgcac aattttcagc gcaatctcca gacgtgccgc cagtgaggcg
    11641 ctgaacgtgg tggtgcgttt ttgctgtgca tgtcgcgcaa ttgaaggcaa tgaatcaaca
    11701 atcgctgacc agcgttctcc gtcaatggca cgagaaatcg cccatgcgat gactaccgcg
    11761 cctgcaaccg ccagatcgga tttatgggtc gggctggacg ccagcgccac atcatcaata
    11821 aaggaatcaa catcacgcgc tggaagcaaa caacctaatg gcgagacgcg catcgctgcg
    11881 ccgttggtta cgccgttgtt ttccagttca gcaacgggtt taccgtcgcg aatggcgtta
    11941 agcgcaatct tcgaggtcgg acctagtacg tttttgttaa aggcgtcgaa acgcaacgcc
    12001 cagtcgagaa tattacgccc aatcagattc ggatcgatct tgccttcaca ttccagtaac
    12061 gcatccgcca gacacaggcc atcgaggtat cgtcggtgaa ttcggcgcgg ttaaaataac
    12121 aggccgcgtt attctccttt ggtccaggca gaaaacggtc aatccagcca aagtgtgctt
    12181 taacgcggct gcgtgtccaa agctcggagg gcatccccat cgcatcccct aacgcctgcc
    12241 cataaagagc accgagaata cgttctgttt tcatttaact tccccttgtg tcaacgcaat
    12301 atctcgatca tcgaccttga tagccgtaat ttcgttgtcg gattcgcgga aaaaaatcat
    12361 gaacagcacg gcgataatgg caatcatcac cgcgccgaaa gtccacatcc cggaccagtt
    12421 gaaagtcagt ccgtttaccg gttcctgata agcgaacatc ttttccatca tcacaccgcc
    12481 aagacgatag ccgagcaaac tgccgaagcc ctggcagcag agcgtgatca gcccctgcgc
    12541 agcggtacgc atatgcacgg gggctttttt atcgacatag atgtaagcgg taacgtagta
    12601 aaaatcgtaa cttacgccgt gaagcaaaat accgaggaac agtaacgcgt aggtgaaata
    12661 ttcatccgca ctaccgtaaa taaagaagcc atagcagatc gcagcggtga ccagaccaag
    12721 caataatacc tttttgatac caaagcgttt agtgaaaaac ggcaatgcca gcataaagaa
    12781 gatttcagag aactggccga gcgtcatcca gccggtggcg tttttcatgc caacttcggt
    12841 cagataaccg ttggcaaaga tgtaatagaa cgccagtggc atcgcaaaca ggaatgaaca
    12901 gaagaaaaag acgaggaagt ttttatcgcg cagcaggatc agcgcatcca ggccgagcat
    12961 gactttaatg tccattttgc cggtgctttt tggcggcgtg tcgggcagga aaaacgcaaa
    13021 cacaccgagc agagcagaac ttccggcggt aatcagcagc gggatgttag tcggtgagat
    13081 atcggcatac cccagcattt gcggcaagaa accacatgcc agaccggagg caatccagcc
    13141 gatagtgccc atcacacgaa tgcgtgggaa atcacgctcc acatccggca cgttggcaaa
    13201 agcgatgctg ttagtcagcg caatggtcgg catataggtt agcgagtagg ccagcagtaa
    13261 cggaaagaac ccggcaaaag tggtctgttg cgcagcgaaa tacatcagca cagcaccagc
    13321 gaacatcaat accgccagca ctttctgcgc cgagaaaaag cggtcagtga tggagccaac
    13381 cagaattggc gacaggatcg ccgcaatggc tgtgcaggca tacgaccagc caatttctcc
    13441 ggcactaaaa ccgcttttac ttaaccacaa ccacaatggc acaaaccacg cgccccagat
    13501 aaaccattca acaaacatca tgaacgacag ctttgctgtt gttttcatct tttaattcct
    13561 tcatgacagg taatgtaagg tacgcgatga caataccaca taataatgcc ttttgaatac
    13621 ctttgcggtg aaaacttgac cgcgctaaca ttttttctga tgaatcgagc caacagaaaa
    13681 cgctgaaaaa acatccaaaa gatggaaaaa ctcgcccgta acgactagga ttatgattgc
    13741 ccgcaaaagg cgggccagga caatccatag ccgatatcca atcggaattt acgggagcat
    13801 agtaatgaca gatattgcgc agttgcttgg caaagacgcc gacaaccttt tacagcaccg
    13861 ttgtatgact attccttctg accagcttta tctccccgga catgactacg tagaccgcgt
    13921 gatgattgac aataatcgcc cgccagcggt gttacgtaat atgcagacgt tgtacaacac
    13981 cgggcgtctg gctggcacag gatatctttc tattctgccg gttgaccagg gcgttgagca
    14041 ctctgccgga gcttcatttg ctgctaaccc gctctacttt gacccgaaaa acattgttga
    14101 actggcgatc gaagcgggct gtaactgtgt ggcatcaact tacggcgtgt tggtgtcggt
    14161 atcgcggcgc tatgcgcatc gcattccatt tctcgtcaaa cttaatcaca acgagacgct
    14221 aagttacccg aatacctacg atcaaacgct gtatgccagc gtggagcagg ccttcaacat
    14281 gggcgcggtg gcggttggtg cgactatcta ttttggctcg gaagagtcac gtcgccagat
    14341 tgaagaaatt tctgcggcct ttgaacgcgc gcacgagctg ggcatggtga cagtgctgtg
    14401 ggcctatttg cgaaactccg cctttaagaa agatggcgtt gattaccatg tttccgccga
    14461 cctgactggt caggcgaacc atctggcggc aaccataggt gcagatatcg tcaaacaaaa
    14521 aatggcggaa aataacggcg gctataaagc gattaattac ggttataccg acgatcgcgt
    14581 gtacagcaag ttaaccagcg aaaacccgat tgatctggtg cgttatcagt tagctaactg
    14641 ctatatgggc cgggctgggt tgataaactc cggcggtgct gcaggcggtg aaactgacct
    14701 cagcgatgca gtgcgtactg cggttatcaa caaacgcgca ggcggaatgg ggctgattct
    14761 tggacgtaag gcgttcaaga aatcgatggc tgacggcgtg aaactgatta atgccgtgca
    14821 ggacgtttat ctcgatagca aaattactat tgcctgatac gcctcatacg aacagcacca
    14881 ccccgtagga cggataagac gtttacactg catccggcaa cctatgcctg atgcgacgcg
    14941 gaagcgtctt atcatgccta catagcactg ccacgtatgt ttacaccgca tccggcataa
    15001 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    15061 tcgaacaatt ttcatattgt tgtttttgtg atcattatct cgacatttaa aagcaaataa
    15121 tttcattata ttttgaaatc gaaagcaaac gacaagatat gaaaatgtac gtggtatcga
    15181 caaagcagat gctgaacaac gcacagcgcg gcggttatgc ggttccggca ttcaatattc
    15241 acaatctcga aacgatgcaa gtggtggtgg aaaccgctgc caacctgcat gcgccggtca
    15301 tcatcgccgg aacgcctggc acatttactc atgctggtac agaaaatctg ttggcgctgg
    15361 tcagcgcgat ggcgaagcaa tatcaccatc cactggcaat tcatctcgac catcacacga
    15421 aatttgacga tatcgctcag aaggttcgtt ctggcgtgcg ctcagtcatg attgacgcct
    15481 cgcatttgcc ttttgcgcaa aatatttcac gggtcaaaga ggtggtggat ttttgccatc
    15541 gctttgatgt cagcgtcgag gcggagctgg ggcaacttgg cggccaggaa gatgatgtgc
    15601 aagtcaatga agtcgatgcg ttgtacacca accccgcaca ggcgcgtgaa tttgccgagg
    15661 caaccgggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg tatgccagcg
    15721 caccggtgct tgatttttct cgactggaga acattcgcca gtgggtgaac ttaccgctgg
    15781 tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc aaactgggga
    15841 tatgcaaaat caacgttgca acggagctga aaaatgcctt cttgcagtcg ttaaaaaatt
    15901 acctgaccga gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg gctaaatccg
    15961 caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc agggcataac
    16021 gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca taaagctggt
    16081 gaacatatcg gcatatgttc agtctgttct gcccatccgt tggttatcga agcggcgctg
    16141 gcatttgatc gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa ccaggtcaat
    16201 caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt ttttacgatt
    16261 gccgataaag ttgggtttgc acgcgaacgc attattctcg gcggcgatca tctgggacca
    16321 aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga gctggtaaaa
    16381 gcatatgttc gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc ctgcgcgggg
    16441 gataccatac cgttagcacc agaaacggtt gcggaacgag ctgctgtgct ttgctttgct
    16501 gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat tggcactgaa
    16561 gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac ccatgttgaa
    16621 gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg gctgacagag
    16681 gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga tcacagcaat
    16741 attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga aaacacccga
    16801 atggtttatg aagcacattc taccgattac cagacccgga cggcttattg ggaattagtc
    16861 cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa cctttgcttt acgcgaggcg
    16921 atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg cagcggttgc
    16981 ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa atattatcgc
    17041 acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg tattcgttat
    17101 tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa tctgcaaggc
    17161 gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga acgcattcag
    17221 tccggggaat tatcagcaat gccgcatcag cttattatga ataaaattta tgatgttttg
    17281 cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct gtttgttcgt
    17341 agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa tgagatgctc
    17401 gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga agcagaattc
    17461 cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga ggcgattcat
    17521 gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt tcagcaagcg
    17581 gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga aaatccgcag
    17641 cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga tatcgtcgag
    17701 acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta tgttttagat
    17761 tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg caagattatt
    17821 gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat taaagagttg
    17881 tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga aatagaaacc
    17941 tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag ttttggcgat
    18001 attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc attacaaaat
    18061 aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg ttatattctc
    18121 gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa aatattaggc
    18181 atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt tgttggcatt
    18241 ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc gatggcggaa
    18301 aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc accaatgacc
    18361 tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa cgtggcgatg
    18421 ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg gcatatgacc
    18481 ttcaccggcg cgttgctgca cctggcaacc ggttcatgga tgatagggat ggcgggtgtg
    18541 gtaattcacg cggcgtttgt ttataagctc tgcgactggt ttgcccgcga tacccgaaat
    18601 ttctttgagc tggaaggtat tgctattccg cacggtacgt cggcgtatat ggggccgatt
    18661 gcggtgctgg tcgatgctat catcgagaaa atcccaggcg ttaaccgaat taaatttagc
    18721 gccgacgata ttcagcgcaa atttggccct tttggcgaac ctgtcaccgt gggttttgtg
    18781 atggggctga ttatcggcat cctcgcgggt tacgatgtca aaggtgtatt gcagctggcg
    18841 gtaaaaacgg cggcagttat gctgctgatg ccacggatga ttaaacccat catggatggt
    18901 ttaacgccca tcgctaagca ggcccgtagt cgtttacagg cgaagttcgg cggtcaggag
    18961 ttcctgattg gcctggatcc cgcattactg ctggggcata cggcggtggt atcggcaagc
    19021 ctgattttta ttccgctcac cattttaatt gctgtttgtg ttccgggtaa tcaggtactg
    19081 ccgtttggcg atcttgccac tatcggcttc ttcgtggcga tggcggtcgc ggtgcatcgt
    19141 ggaaatctgt tccgcacctt aatctcgggt gtcatcatta tgagcatcac cctgtggatc
    19201 gcgacgcaaa ctattggttt gcacacccaa ccggcggcta atgctggggc gttaaaagcc
    19261 gggggtatgg tggcttcaat ggatcagggc ggttctccca ttacctggtt actgattcag
    19321 gttttctccc cgcaaaatat tcccggtttc attattatcg gcgcaattta tctgaccggt
    19381 attttcatga cctggcgtag agcgcgtggt tttactaaac aagagaaagc cgttctcgca
    19441 gaataatttt tacctgaggg ggaggttatc ccccttaaca atcaggagtt tttatgaaat
    19501 cagtagtgaa tgatactgat ggtatcgtgc gcgttgcaaa aagcgtcatt cctgaaatta
    19561 aacatcagga tgaggtgcgg gtaaaaattg ccagctcggg cttatgtggt tccgatttac
    19621 ccaggatatt taaaaatggt gcacattatt atccaataac gttaggccat gaatttagcg
    19681 gctatattga tgcggtggga tccggtgttg atgatttaca ccctggcgat gcggttgcct
    19741 gtgtgccgtt attaccctgt tttacttgtc cagagtgttt gaaagggttt tattcccagt
    19801 gcgcaaaata tgattttatt ggctcgcggc gtgatggtgg atttgctgaa tatattgtcg
    19861 ttaagcgaaa aaatgtcttt gctctaccca cggatatgcc tattgaggat ggggctttta
    19921 ttgagccgat taccgttggc ctgcatgctt ttcatttagc gcaaggttgt gagaataaaa
    19981 acgttattat tattggtgcc ggaaccattg gcctgctggc tattcagtgc gctgtcgcgc
    20041 tgggagcaaa gagtgtgacg gcgatcgaca ttagctcaga aaaactggca ctggcaaaat
    20101 ctttcggtgc gatgcaaaca tttaacagta gcgaaatgag cgcgccgcaa atgcagagcg
    20161 ttttacgcga actgcgcttt aatcagctta tcctcgagac ggctggcgta ccgcaaactg
    20221 tcgaactggc ggtagagatt gccggtcctc atgcccaact ggcgctggtg ggcacgttgc
    20281 atcaggatct gcatttaaca tcggcaacgt ttggcaaaat attgcgtaaa gagctgacgg
    20341 ttatcggcag ttggatgaac tattccagcc cttggccggg gcaggagtgg gaaacggcga
    20401 gccggttgct gacagaacgt aagttaagcc tggagccatt aatcgctcac cgtggaagct
    20461 ttgaaagctt cacccaggcg gtgcgtgaca tcgctcgtaa tgccatgccg ggcaaagtgt
    20521 tgctcattcc atgaaaccgc gggccagcgt gatgctggcc cggtattgtg caaaacagat
    20581 cattcaccaa tggtccccct tcgtttacac tagccacaat tgaatatggg taaatgacga
    20641 tgaattcatt cgagcgaagg aataagatca tccaattagt gaatgaacag ggaaccgtgc
    20701 ttgttcagga tctggcggga gtatttgctg cctcggaagc gacaatccgt gccgatttgc
    20761 gctttctcga acaaaaaggc gtggttacgc gctttcatgg cggtgcggcg aaaataatgt
    20821 ccggtaatag tgaaaccgag acccaggaag tcgggtttaa agagcgattt cagctcgcca
    20881 gcgcgccaaa aaacagaata gcgcaggcgg cagtcaaaat gatccatgaa gggatgaccg
    20941 ttattctcga cagcggaagt accacaatgc ttatcgccga aggattaatg accgctaaaa
    21001 atatcacggt gattaccaac agtctcccgg cagcgtttgc cctttccgaa aataaagaca
    21061 ttactctggt cgtctgtggt ggcacggtcc gccataaaac gcgctcgatg catggttcca
    21121 ttgccgagcg ttcattgcag gatattaatg ccgatttaat gtttgtcggt gctgatggta
    21181 ttgatgcggt taatggtatt acgaccttta atgaaggtta ttcgattagc ggggcgatgg
    21241 tcaccgccgc aaataaagtg attgccgttc tcgattcatc gaaatttaat cgtcgcggtt
    21301 ttaatcaggt actgccaatc gaaaaaattg acattattgt taccgatgat gctgtgtcag
    21361 aggtggataa attggcattg cagaagacac gggtaaaatt aattacggtg tagtattgtt
    21421 ctgtatgccg gataaggtgt ttacaccgca tccggcatta cggattctcc ctggttcaag
    21481 agagaatagt ttgattaacg caataatgga caatctggtg gtaatcgaca acgcaacgcc
    21541 gccggaagta tttcaatatg gaagttttgc ccactcaacg gttcgccatc aagattaaaa
    21601 gtgatttcgt gtggggcttg tatatcaaac cacgacgaag cgccttcgat aatattcggg
    21661 ttatcttcgt cagattttaa ggttgatacg agagccggaa gaatttcatc gccggtaaaa
    21721 atgcgcagtt gcagcaagcc atcgttaatt aacgcgttcg ggcacaattg ttgaccgcca
    21781 ccggcctgac gcccgttacc aataccaatg accagggcgt caccttgcca gtgaaagttt
    21841 tcaccgcgga tttcacaacg gtccggttgc agagtatcca tgcgcattaa gccatgaatg
    21901 atgtaagaga cgccacccag cgcggctttt aatttttccg gcgtttctgt ggtaatacgc
    21961 gtcccaaatc cgcctgtcgc catattaata aaacaggttt gtttgttgac ctgcgccata
    22021 tctatcgcaa tggcattacc ggcaattgcc agtttcagcg ccttatccag tgcctcagga
    22081 atccctacac tggtggcaaa atcattggca gttcctaatg gcaaaattcc cagcgcgggt
    22141 atgtcatccc cctcacactg aatcaacgcc gtagaaactt cattaatggt gccatcgcca
    22201 ccaccggcaa tcaccgttgc gacgcccaac ttccgggcct cctctacaaa tcgtgcggca
    22261 tcacctttct cccaggtgac ccgcacatgg atcgtcattc cttcctcacg caacagcata
    22321 attgcttcgc gcaagggtag attgtcagta cttttaccat taagaatcag taagctggcg
    22381 ggaaattctg ccatgatcgt atgtgccttt atgattggtc tgtagatagt gtagagcaga
    22441 aaacaaaagg tggagtcaga acaggatgaa agtcggagga taaaaagtca gttcaagtat
    22501 ggaggattca gtggttacca ccaggagctt gcaatgggga cagcgaatac taacggctgc
    22561 caccgataaa tttcaaaaaa gagcatatac ctaatattca actaaacagt ggcatcttca
    22621 atataatata ttaaagcccc catggagtta ccctgaaggg cctcaatgtc cgtaattcct
    22681 acttatgtag gaaatgttgt acagaacatt tattataatc ctattcaatt ataataatca
    22741 tgccattatt atatttaaac actagagagt gtcgttggta tttaatgggg gaaggtgaga
    22801 tgaaaaagat agctgctata tcattaatta gtatttttct tatatctggt tgtgctgtgc
    22861 ataatgatga aacaagtatc ggtaaatttg gtcttgcata taaaagtaat attcagcgta
    22921 aactcgataa ccaatactac accgaagccg aagcttcttt agccaggggc agaatatctg
    22981 gtgcagaaaa tatagtaaaa aatgatgcag cccatttctg tgttactcag ggcaaaaaaa
    23041 tgcagatagt tgacctgaag acagaaggtg caggattaca tggcgtcgct cgtctgacat
    23101 tcaaatgtgg agagtgagaa tatttttttg taagcgtcaa acatgcgcgt tctggctgtg
    23161 cgtagccgga acctgggcga gcacgatacc gttacgtgaa aggcattgtg ctatgaaggg
    23221 ggattctatc gatgtggtca atggaagacg gttatcaggg atcaatcctt ctttacagct
    23281 tatgtacaaa aaataagccc gtgtaaggga gattacacag gctaaggagg tggttcctgg
    23341 tacagctagc attttatgga ttatgttttt cagcgaaacg gatgataacc ttaataaatg
    23401 cagctgtatg tgatcggttt ctaagaattt tccatccggg aaaaataatc gaaattaatc
    23461 acttaccgtg ggggttacgc gtggtttccc cggagaaatt acgcattagc agcgcgtaat
    23521 ttagctcaag atcctgcggg accgggagcc acacagtata accatcgcct ggtgcgaccg
    23581 gcatagcttc gcctttggcg ttttccatgt gctcaagggt aaagttaatg ttgccttgcg
    23641 gcgtcatcag ctcaagactg tcgccaacgg agaatttatt tttcaccgct accgctgcga
    23701 ggtccccctt gcgctcaccg gtaaactcac caacaaactg ctggcggtca gaaactgaat
    23761 aaccgtattc gtagttctga taatcgtcgt gagtatgacg acgcaggaaa ccttcggtat
    23821 agccacgatg cgccagacct tccagagttt ccagcaggct ggtatcgaac ggttttcccg
    23881 cagcggcgtc atcgatagct ttgcggtaaa cctgtgcggt gcgtgcgcaa tagtagaaag
    23941 atttggtacg accttcgatt ttcagcgaat gcacgcccat tttggtcagg cgttctacat
    24001 gggcgatggc gcgcagatct ttcgagttca tgatgtaagt gccgtgctca tcttcaaacg
    24061 cggtcatata ctcgcccgga cgctgggctt cttcgatcat aaacactttg tcggttggcg
    24121 cgccgatacc cagcgtcggc tcaacatttt gcaccggaat cggctcgtac ttgtgtacga
    24181 tgttgccaac gtcatcttct ttcccttcct ggacgttgta ctcccagcgg caggcgttgg
    24241 tgcaggtgcc ctggttcggg tcgcgcttgt tgatatagcc agagagcagg cagcgaccgg
    24301 agtaggccat gcacagtgcg ccgtgaacga agatctcgat ctccatatcc ggcacctgat
    24361 tatggatctc ttcaatctct tccagcgaca gctcgcgaga gaggatcacg cgggtcagtc
    24421 ccatttgctg ccagaatttc accgtcgccc agttcacggc gttagcctgc accgagaggt
    24481 ggatcggcat ttcagggaag tgctcacgca ccagcataat cagccctgga tcggacataa
    24541 tcagcgcatc cggccccatt tccaccaccg gtttcaggtc acggataaag gttttcagct
    24601 tggcgttgtg cggtgcaatg ttgaccacga cataaaactt tttccccagc gcgtgggctt
    24661 cattgatgcc gagctgaaga ttttcgtggt tgaattcgtt gttgcgcaca cgtagggagt
    24721 aacgcggctg gcccgcataa acagcatctg cgccataagc gaaagcgtaa cgcatatttt
    24781 tcagcgttcc cgccggggaa aggagttccg gtttaaacat aattctctcg ttctgatgac
    24841 aggtcagatc cgcttcacct gatgaagcgg ttaggggagg cccccacttt aagggcgggc
    24901 attgtagcgc tgtagggggg atctggtaaa gcgccggacg ccaacaaccc acgcccggca
    24961 tcgtaattac gtattgtctt tcaccgtctg gcttgtgcca ttggctttaa cggaggcaat
    25021 tccggtttca cgagattgcg cggtggcgta catctggctg gagccgataa tttgatgatt
    25081 agcggctttc agattgaaat agaatttgcc gttgcttgcc gttttttctc atagcgctct
    25141 tcttgcgggc tgttgctacg caccgacgcg atgccctttt ccgcagaggc ttttgaggta
    25201 tacagctcgc tggtgaggat agtctcacca ttgcccgctt ttagcacaaa ccggaactga
    25261 ttatcactgc tcttactgag ttcaaaccaa ccagccataa tgactccttg attgaaattg
    25321 cttatcgaaa acagcatcta aaggtgctgt ttactgatta aagtatgagc gattttctga
    25381 tgcctgccag ggcggagaga taatgtcagt ttgctgacca tgcgtggaat aatgttgccg
    25441 gatcgggtct gaacgccatc tccggctaac aaagtaatgt cgctaaaact aaacgatgcg
    25501 gcaggcgtcg gcttcccagc ggtaaccaac gccgtaaacg gcgcggataa atgactgttc
    25561 ggcgtcgaga gattccagct tgcggcgcag atttttaatg tggctgtcaa tggtgcggtc
    25621 ggttactacg cggtagtcgt cataaagatg attgagcaat tgctcgcggg agaacacttt
    25681 tcctggttcg tgagagagcg ttttaagcag acgaaattcc gcaggcgtca ggtcaagcat
    25741 tttaccgcgc catgaagcct gaaaacgacc ttcgtcgata atcaacgggc tttcggcatc
    25801 ctgttgctga agttcgcgtt gcggtttaca acggcgcaaa atggttttga cacgcgctac
    25861 cacttcccgt gggctgtacg gcttgcagat gtagtcatcg gcgccaatct ccagccccag
    25921 caggcggtcg atctcttcga tttttgccgt caccatcacg atcggaacat cggaaaaacg
    25981 acgaatttcc cggcataatg tcaggccatc ggtgccaggg agcatcagat ccaacaggat
    26041 cagatccggt ggtgtctggc gcacatacgc cagtacctga tcgccgtggc tgataagcgt
    26101 cggcgcatag ctcgcagcac gcagataatc aatgagcaac tgccccagct tcggttcatc
    26161 ttccacgatc aaaatacgcg gtgtgttttc gtcgattggt aactcggtca tacttctctc
    26221 tgtaaatccc gttccagcgg taactctact gtaatgctta ccccgccaaa aggcgaatgg
    26281 gcggcaataa tgcggccatt atgggcttca acaatgttca ggcaaatcgc cagccccagc
    26341 ccggaaccgc cgctggcgcg gttgcgggaa ccttcggtgc gataaaaacg ttcaaacaat
    26401 ttttgtagct gatcgtcact gacacctggc gcactgtcgg caaaggtcag gcgcaccgtt
    26461 ttgtcgtgct gctcggcaga gattttcagg ctgccgccgc tgtcagtgta gcgcaggctg
    26521 ttttccagta agttattgaa taactgcatt aaacggtcgc gatcgccaaa tacggtgata
    26581 ctgtctggca gggaaaattg cagtttcagg ccacggctgg cgaatcgttc gcgaaatgcg
    26641 ccgcccgcca cttccagcag tgggatcaaa tctaccggtg ctttttgata ggcgagagcg
    26701 ccttcatcag acatcgacaa ctgatgaaga tcgtcaacca gtttggtcag tgtaccgacc
    26761 tccgcctgta aagacgccac cgtctccggc gtgaatttgc gcacgccatc ctgaatggct
    26821 tccagttcac cgcgcagcac cgctaatggc gtacgcagtt cgtgagaaat atcggccata
    26881 aaatcgcgcc gcatttgctg gtttttctcc agcgtgctgg cgagctggtt gaagtcttgc
    26941 gccagtttgc ccagttcatc ttcactggtg ggcgttacgc gggtagtgaa atcgcccgcc
    27001 gccagtttgt gcgtgccatc gacaagtcgt tttaccggtg ccagcaaacc gcgcgccagc
    27061 agaaaggtgg cgagcgccgc gagtaacgtt gccagggcga cgatcagcca gctggtttgc
    27121 cgctgttgtt tatcgaaatt gatatcagta ttgcgggtca gccgctcaac aggggaggca
    27181 atcaccgcgc ccacttccgc accgttgacc agaatgggtc gccgtgtacc gtcaggtgga
    27241 atcggcgctc gcggaccaac cagtactttg ttgttttgat cgaccaccca gaactgggta
    27301 cgccagccgt gcggcggcat acccgggccg ggtttatctt ccgaattatc gtgttcaaat
    27361 gaacgcagga tctgaaagac aaagcgatcg ttgttgcgca ggaagcgcca gttaccatgc
    27421 tgcgcatact gctcgccaag cgcatcactt aacagctgta atcgctgttc attgccgtgt
    27481 ttgatgtaat caataaagcc acgctcaaaa ctgatacgca ccgcccagtg catactgatc
    27541 agcaagacaa tgcaggtggc gaaaatcgcc agaaacagtt tgccggtaat accgggtcgc
    27601 cagaacttca ttgcgcgctc ctttttcgcc gcgaaatagc tacattttga tgcgtatcgt
    27661 tcggcactct ggcaaagatg aacgccggaa gggcgatgat aaacgccatg ctaagccagg
    27721 tgtacataaa gacggtttgt gtggtgccgc tgtcgacgct gatatgctgc gaaccaaaaa
    27781 gccccagcaa cagcccggcg atggtgacgc cgatactcat cgacaattgc ataatcatcg
    27841 acagcaggct gtttccgctg ctcgccagat tgtccgggag atctttcagc gtcagggtgt
    27901 tcatggagga gaaacgggtc gagttgacca tcccttgtaa aaacaggacg aacggcaaaa
    27961 cgtagtacca gcccaacaga gcggtagtca taaacaacag ggtgaccagc gacagaccca
    28021 gcgtggtcgc taccagtacc cgacgataac caaagcgatt caccacctgt accacaattc
    28081 gcttcattcc catgctgcca agcaccatcg ggatcatcat cagtccggca tgaaacggcg
    28141 agaaaccgag gccaatttgc aggaaaaccg gtgtcataaa gggcaacatg ccactgccaa
    28201 tacgtccggc aaagctcccc gccaggccca gcgaaaaggt acgagtgcgg aacagtttca
    28261 gactgaacag ggcgcggttg ttatttctgg cgtgcagcag ataaagcacc agtgccacca
    28321 cgccaactgc gaccaggcca gcaatcgcca gcggcgataa acctgtacct ttactgccgt
    28381 ccagcgccag ggttaatacc gccatgccaa ccgccagcaa taaaaagccg gaaagatcaa
    28441 agcgccgcgt ctgcatggtg tagttcggca ttaacatcaa tgtggcgatc gcaccgataa
    28501 tccccaccgg aatgttgatc aaaaagatcc agtgccacga cgcgtattct accagcagac
    28561 cgccgagcgc cggaccgagc agcggaccga cctgaccggg taacgtgaca aaggtcatcg
    28621 ccgccatata ttgctcgcgc ggtacgattt tcatcaccgt caacctgccg accggcacca
    28681 tcatcgcacc gccaacgccc tgtaacgcgc gtgccagcaa cagttcgttc agcgtgccgg
    28741 aaagcgcgca aaacagtgaa ccgagagtaa acagcacgat ggcggtaaag aaaatattgc
    28801 gcacgccgac tttgtccgcc agccagccgc tggcgggcag catcaccgcc acggtcagca
    28861 cataagagac aatgaccatg tgcatatgca acggactttc cccgaggctt tgcgccattg
    28921 agggaagggc ggtgtttacg atggtggtgt ccagcgactg cataaagaag ccgaaagcca
    28981 caatccataa ttgccagcgg gtgctgtcgg gaagatctgt catttactcg gttaccgttt
    29041 gtttaggttt acgcgaaaaa cgcagccgca gacggtcgaa aaagagatac accaccggcg
    29101 tggtatacag cgtcaggagc tggctcatca ccagaccgcc gacaatggtg atctccaggg
    29161 gttgccgcag ctccgagccg tcaccgcccg acaataccag cggcagcgcg ccaaacagcg
    29221 ccgccagggt ggtcatcata atcgggcgaa aacgcagcag acaggcctgg aaaatagctt
    29281 cctgcggcgt caggttaccg tgccgttggg cttcaagcgc aaaatcgacc atcataatgg
    29341 cgtttttctt cacgatgccg attaatagca tgatccctat cagggcgatt aggctgaacg
    29401 gggcattgaa cagctccagc gccaacagcg ctccaacgcc cgccgagggc agggtggaga
    29461 gaatcgtcag cggatgtacg taactctcgt aaaggatacc cagcacgata tacaccgtgg
    29521 cgatggcggc gataatcagg atcacctgcg agttcatcgt ctcctggaaa acctgcgccg
    29581 tgccggcaaa actgccgcgc accgtcgaag gcacaccaag ctgggtcatt gcgcgatcga
    29641 tcgccgcact ggcgtccgag agcgattttc cggtcggcag gttgaacgag atggtcgagg
    29701 ccgccgataa tccctgatga ttcaccgata gtggggcatt cgccggttgc catttagcga
    29761 aatacgacag cgggatcgct ttgccttcgt tattgataac gaacattttt tccagcgcac
    29821 tgatgtcctg ggtatagcgc ggatccactt ccatcaccac tttatactgg ttcatcggct
    29881 ggtaaatggt cgagatttgc cgctgaccga aggcgttatt taacagactg ttggcggcct
    29941 gtacgtcgat tcccagccgt gccatggtgt cgcggtcgta aaccagattc atctccgcgc
    30001 cgttatcctg ctgatcggag ttcacgtccg ccagttccgg caacgtcgcc agttttttgc
    30061 ggattttcgg ctcccattcg cgcagtgccg ccaggtcgtc ggataacaac gtgtactggt
    30121 agctggcgtt cgactgacgc ccaccaacgc gaatatcttg taccgccatc aggaacagat
    30181 tcgcccccgg ttctttcgcc agcttcacgc gcagacggtc gataatttgc tgcgccgttt
    30241 cgctgcgttc gtcgcgtggc ttgagggtga taaacatcat cccactgtta actcgtgaac
    30301 cgccggtaaa gccggtgaca ttatccactg ccggatcgtc acggataatt ttcatgaaat
    30361 cctgcaattt gccgcgcatc gcctgaaacg aaatactctg atccgcctgt atcccaccca
    30421 tcagtacgcc ggtgtcctgc tccgggaaga aggttttcgg gatcgagata ttcagcgcaa
    30481 tggtgccaag cagcaccacg cccaccagac gggtatgatt gagcacccat ttcagcgact
    30541 tgccgtagcc ttgttgcagg gctaccaaca tgcggccaaa accacgcagt cgcttttgct
    30601 cacgcggctt gctggctttt agcatccagc cgcacatcat tggcgttagc gtcagggaaa
    30661 ccagcaacga aataccaatg gcgacagaaa gcgtcacggc aaactcgcgt aacagtcggc
    30721 ccggcaatcc gcccatcaac agcagcggca ggaacaccgc caccagtgac agactcatcg
    30781 acagcaccgt aaagccgact tcgcgagtac cttgcagcgc ggcctgtagt ggtttcatcc
    30841 ccgcttccag atgacgggca atgttttcca gcaccacaat ggcgtcatcc accacgaaac
    30901 cagtagcaat ggtgagcgcc atcagcgaaa gattattgag actgaatccg cacaggtaca
    30961 tcgccgcaaa cgtaccaatc agcgaaaccg gcaccgcaac ggcgggaata atagtggcgc
    31021 gacccgagcg caggaataaa aagaccacca gaatcaccag cgccaccgag ataatcagcg
    31081 tttgctcgac ttcttccagc gaggcgcgaa tggtggggga gcgatcctgg gcaatttgca
    31141 gatcaatcgc cgccggaatg gtctcctgca actctggtaa ttttgcccgg atgctgtcta
    31201 ccgtctggat aatattggct tccggcagtt tgcggatcat cagtaaaata gccggtttgg
    31261 cgttggtcat cccggcgttg cgcacatcct gcactgagtc agtcaccgtc gccacatcgc
    31321 ccagacgaac cgcgccgccg ttgttgtagt gaataatcag cggctgatat tcagcggcgg
    31381 ttttcagttc atcgttggtc tggatctgcc agcggtgagt gccatcttcc agcgccccct
    31441 gcggtttacg cacgttggca ttgctgatgg cagtgcgtac gtcgtccagc gacacgccct
    31501 gattaaacag cgcctgcggg gtcagcccga cgcgcacggc gggcagcgag ctgccaccga
    31561 catcgacatc accgacaccg tcgatttgcg aaatcgtcgg agccagctgc gtcgaggcga
    31621 aatcgtacag ttcaccctgc gaataagtat cggatgtcag cgtgaggatc ataattggcg
    31681 catccgacgg gttcgctttg cgataggtcg ggcggctggg catcccgctg ggcagcaaac
    31741 tttgtgcagc gttgatcgcc gcctgcacat cacgcgctgc gccgttgata tcccggtcaa
    31801 aatcaaactg caaaataatg cgcgtgctgc cgagcgaact gctggaggtc atttcactaa
    31861 ccccggcaat gcgcccaagt gagcgctcca gcggcgtggc aacggaagac gccattgtct
    31921 ccggcgacgc accgggcagc gaggcgctga ccataatcac cggaaaatcg acctgcggca
    31981 gcggggcgac cggcagcata cggaaaccca gtatgccgca cagggtaatg gcgaccgaca
    32041 gtaaaatcgt cgccaccggg cggtaaatga agagggcaaa aaacttcact tacgcctcct
    32101 cttcatggcg agcaaagcga cttttggtcc acaatgccag gcggtcgaac agcagataaa
    32161 tcaccggcgt ggtgaacagc gtcagcacct ggctgacaat cagaccgccg accatgccga
    32221 tacctaacgg acgacgcagt tccgcgccga ccccggtact caacatcagc ggcagcgcgc
    32281 caagcagagc cgccagggtg gtcatcagga tcggacgaaa acgcaacaga caagcctggt
    32341 agattgcatc gcgcggcgac atgccttgct cgcgctcagc agccagcgcg aagtcgatca
    32401 tcatgatggc gttcttcttc acaataccga tcagcaaaat aatgccgata atcgcaatca
    32461 catccagttc gctaccggca atcattaatg ccagcagcgc gccgacccct gcggtgggta
    32521 gcgtcgagag aatggtgatc gggtgaataa agctctcgta cagaatgccg agcacgatat
    32581 acatcgccac caccgccgcg acaatcagcc agacagtgct gcccagcgcc gactggaagg
    32641 cgagggtgct gccctagaat tgcgtggtga tatccaccgg cagattcagc gtcttttcgg
    32701 tgtccataat cgcctgcacc gcatcgccca gcgaatagtt atccggcacg ttaaaggaga
    32761 tggttgttac cgggaactga tccagatggt tgatggaaag cggcgcaaaa cgctgctcaa
    32821 ttttggcaat tgagcttaac ggcaccacgc caccgtcgct gctggtcagg cgaatggtat
    32881 ccagcgccgc gaggcctggg gtattttcgg tattgtgctc cagcaccacg cgatactggt
    32941 tagcctgagt ataaatagtg gaaatcagtc gctgaccaaa cgcgttgtac agggcgttat
    33001 cgacgtccgc catgctgatc cccagacggc tggcgctgtc gcgatcaaca ttgacatacg
    33061 ccaccagccc tttgtcctgc cagtcgctgg agacatcaga aagctgtggc agttgctgga
    33121 gtttttccat caactgtggc acccaggtac tgagcgcatc cagtgacgtg gcctgcaagg
    33181 taaactggta ctgggtgcgg ctgacctgag tatcgatggt caggtcctgc gttggttgca
    33241 ggaagagatc gacgcccggc actttatcta ccgccgtttg cagacgggcg atgacttttt
    33301 gcacccgatc atcacgttca tccaacggtt tgaggttgat ttgtaaacgt gcactattca
    33361 gcgacgggtt agtgccatca acgccaacaa atgaggtcag gctttgcact gccggatcct
    33421 gcaaaatcac gtccgcgacc tggcgttgtc gctgggccat attggcaaag gagctggatt
    33481 gcggtgcctg caaagtgccc tgaataatgc cgttgtcctg taccgggaag aaacctttcg
    33541 gaatgaacac ccacagcagc acgctaagca gcagcgtgct gagtgccacg cttaaggtca
    33601 gccacggatg attcagcact ttcgccagtc cacgaccata ggcggcgatt atcctgtcga
    33661 acattttttc cgaggcacgg gagaagcggt tctgtttacg caacgactcc tggctgagca
    33721 tccgcgcgca catcatcggt gtcagggtca gcgacaccac cgctgagatc aaaatcgcta
    33781 ccgccagggt aatagcaaat tcgcggaaca gtcgcccgac gatatcgccc ataaacagca
    33841 gtgggatcaa caccgcaatc agtgagaagg tcagcgagat aatggtaaag ccgatttcac
    33901 ctgcgccctt gagcgccgcc gccaacggtt tttcgccttt ttcgatatag cgggaaatgt
    33961 tttcgatcac cacgatggcg tcatcgacca cgaatccggt ggcgatagtt aacgccatca
    34021 gtgtcaggtt attgattgaa aaatcgagaa acaccataac cgcgaaagtg ccgattaacg
    34081 acagcggtac agcaacaccg ggaatgatgg tcgccggaat attgcgcaaa aacaggtaga
    34141 taatcatgac taccagcgcg atagccatca tcaattcaaa ctgagtatca tcgacggatg
    34201 cgcggatatt ggtggtgcga tcggaaagca ctgtcacctt caccgatttc ggcagactct
    34261 cagtgagctg tggcagcatc tgccgaatgc tgtcggcggt ggagataatg ttagcaccgg
    34321 gctggcgctg aacattcatc acaatggcct gttctttgtt cgcccacgcg ccgagccagc
    34381 tgttttctgc accttgctct acagttgcga catcgcccag acgaattggc gcgccgttct
    34441 ggtaggcgat gattagctgg cgatactctt cggcggattg catctggtcg ttcgcggaaa
    34501 gcgtgaccgc acgggaaggg ccgtcgaggc taccttttgc cgagttaacg ttagcgccgg
    34561 taatggcggt gcgcacggtt tcgctggtca ggccgagggc ggcaatcgcc tgagcgttaa
    34621 gtttgacgcg aacagccgga cgctgaccgc cggaaagcgt caccaggccg acgccggaaa
    34681 tctgcgagat tttctgcgcg acgcgggttt ccaccatatc ttccacttgc gtcatcggca
    34741 tggcggttga ggtgacggcg agcgtcatga tcggcggatc tgccgggttc actttgctgt
    34801 aaaccggcgg gttaggcaga tcgctcggca acaagttggt cgcagcgtta atcgcggcct
    34861 gcacttcctg ctcggcgaca ttgagcggta atgttagctg gaactgcaaa gtgataactg
    34921 acgcaccgcc ggaactttgc gacgacatct gtttcaggcc agacatctgc ccgaactggc
    34981 gttcgagcgg cgcggtaacg gcagaggtca tgacatccgg gctggcacct gggtagagcg
    35041 tgaccacctg aatggtcgga tagtccactt ccggcagcgc cgaaacgggc agggcgcgat
    35101 aaccgataat cccggcgagt aagatcgcca ccatcagcag cgtggtggcg acaggacgca
    35161 taataaacag gcgcgacggg ccgcctgtgc tgctcggggg taacacctgc atcaggagcg
    35221 tgctcctttt ttcgcgtatt cgcggctggt ggctttctct tccggagtgg tggcgctctg
    35281 ggcttccacc acttccactt tcgccccttc ggtcaggcga tcaatgccgt ctgtcaccac
    35341 gcgatcgccc gcagaaatac ctgcacggat caccactttc tgactgtcct gaatgcccgg
    35401 cgtcaccaga tgtttgctga ccttgttttc gctattcagc acccagacaa aatggccttc
    35461 attgcccatt tgcagggcgg ctgttgggat cactacggcg ttttgttcgg tgtcgactaa
    35521 catgcgcgcg ttaacaaact gattgggaaa cagcgcatca tcctgattat taaagcgtgc
    35581 tttcacttta atcgtaccgg ttgtggcatc gatttggtta tcgaggctta acagcgtgcc
    35641 ttcacttaat ttcttcgaat tggtgcgatc ccaggcttct accatcagcg gttttccggc
    35701 tttttgcgcc tgcactacag tagcgatatc gctttccggc agggtaaaga ctaaatcgat
    35761 aggatgcgtc tgggtgatca ccacaattcc ggtggtatca ccactggaga tttggttacc
    35821 aacatcaact tgcttgagac caacgcgacc atcgactggt gcggtgatgc ggctccagtc
    35881 gagttgcaac tgcgcgctgg cgacgcttgc ttcatcagcc ttaatggtgc cttcggtttc
    35941 actgaccagc gcctgttggg catccagctc ctgacgggag acgagattgg tttttgccag
    36001 ttgctgataa cgcgccagat cacggcgggc gttagcaagc gtggctttat cttttgccag
    36061 ttggccctgg gtttgtgcta atgcaacttt gaactggctg gggtcaattt ctgccagtaa
    36121 atcgcctgct ttgacctgct ggccttcctg gaaatgtaac gccatcagtt gaccgtccac
    36181 gcggctgcgc accgtaacag tattagcggc ggtaatggtg ccaagcccgg tgaggtaa
//
LOCUS       DAECOD010000031        35343 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.031, whole genome shotgun sequence.
ACCESSION   DAECOD010000031 DAECOD010000000
VERSION     DAECOD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.031"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            57..2654
                     /locus_tag="KKO15_001590"
                     /pseudo
     CDS             57..2654
                     /locus_tag="KKO15_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            2651..3739
                     /locus_tag="KKO15_001591"
     CDS             2651..3739
                     /locus_tag="KKO15_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBD6360416.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3827..5125)
                     /gene="exuT"
                     /locus_tag="KKO15_001592"
     CDS             complement(3827..5125)
                     /gene="exuT"
                     /locus_tag="KKO15_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBD6360417.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5609..7021
                     /gene="uxaC"
                     /locus_tag="KKO15_001593"
     CDS             5609..7021
                     /gene="uxaC"
                     /locus_tag="KKO15_001593"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBD6360418.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7036..8523
                     /gene="uxaA"
                     /locus_tag="KKO15_001594"
     CDS             7036..8523
                     /gene="uxaA"
                     /locus_tag="KKO15_001594"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBD6360419.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8606..9157
                     /locus_tag="KKO15_001595"
     CDS             8606..9157
                     /locus_tag="KKO15_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBD6360420.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(9162..10406)
                     /gene="sstT"
                     /locus_tag="KKO15_001596"
     CDS             complement(9162..10406)
                     /gene="sstT"
                     /locus_tag="KKO15_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBD6360421.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMTGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11002..11967)
                     /gene="alx"
                     /locus_tag="KKO15_001597"
     CDS             complement(11002..11967)
                     /gene="alx"
                     /locus_tag="KKO15_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBD6360422.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(12238..13236)
                     /locus_tag="KKO15_001598"
     CDS             complement(12238..13236)
                     /locus_tag="KKO15_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6360423.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(13315..14007)
                     /locus_tag="KKO15_001599"
     CDS             complement(13315..14007)
                     /locus_tag="KKO15_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBD6360424.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(14084..14587)
                     /locus_tag="KKO15_001600"
     CDS             complement(14084..14587)
                     /locus_tag="KKO15_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBD6360425.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14672..15808
                     /gene="rlmG"
                     /locus_tag="KKO15_001601"
     CDS             14672..15808
                     /gene="rlmG"
                     /locus_tag="KKO15_001601"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBD6360426.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16093..16407
                     /gene="higB"
                     /locus_tag="KKO15_001602"
     CDS             16093..16407
                     /gene="higB"
                     /locus_tag="KKO15_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBD6360427.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16404..16820
                     /gene="higA"
                     /locus_tag="KKO15_001603"
     CDS             16404..16820
                     /gene="higA"
                     /locus_tag="KKO15_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBD6360428.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16865..18883)
                     /gene="fadH"
                     /locus_tag="KKO15_001604"
     CDS             complement(16865..18883)
                     /gene="fadH"
                     /locus_tag="KKO15_001604"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBD6360429.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGSAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQSCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19109..21460)
                     /gene="ygjK"
                     /locus_tag="KKO15_001605"
     CDS             complement(19109..21460)
                     /gene="ygjK"
                     /locus_tag="KKO15_001605"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBD6360430.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21477..21896)
                     /locus_tag="KKO15_001606"
     CDS             complement(21477..21896)
                     /locus_tag="KKO15_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360431.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21874..22227)
                     /locus_tag="KKO15_001607"
     CDS             complement(21874..22227)
                     /locus_tag="KKO15_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360432.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(22228..22548)
                     /locus_tag="KKO15_001608"
     CDS             complement(22228..22548)
                     /locus_tag="KKO15_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360433.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22682..24115)
                     /locus_tag="KKO15_001609"
     CDS             complement(22682..24115)
                     /locus_tag="KKO15_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6360434.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24178..24627)
                     /locus_tag="KKO15_001610"
     CDS             complement(24178..24627)
                     /locus_tag="KKO15_001610"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBD6360435.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24624..27710)
                     /gene="ebgA"
                     /locus_tag="KKO15_001611"
     CDS             complement(24624..27710)
                     /gene="ebgA"
                     /locus_tag="KKO15_001611"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBD6360436.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRMMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27893..28876)
                     /gene="ebgR"
                     /locus_tag="KKO15_001612"
     CDS             complement(27893..28876)
                     /gene="ebgR"
                     /locus_tag="KKO15_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBD6360437.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29094..29425
                     /locus_tag="KKO15_001613"
                     /pseudo
     CDS             29094..29425
                     /locus_tag="KKO15_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29467..30846)
                     /gene="ygjG"
                     /locus_tag="KKO15_001614"
     CDS             complement(29467..30846)
                     /gene="ygjG"
                     /locus_tag="KKO15_001614"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBD6360438.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31264..32784
                     /gene="aer"
                     /locus_tag="KKO15_001615"
                     /pseudo
     CDS             31264..32784
                     /gene="aer"
                     /locus_tag="KKO15_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32938..33561)
                     /gene="nfeR"
                     /locus_tag="KKO15_001616"
                     /gene_synonym="yqjI"
     CDS             complement(32938..33561)
                     /gene="nfeR"
                     /locus_tag="KKO15_001616"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBD6360439.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33838..34583
                     /gene="nfeF"
                     /locus_tag="KKO15_001617"
                     /pseudo
     CDS             33838..34583
                     /gene="nfeF"
                     /locus_tag="KKO15_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34637..34712)
                     /locus_tag="KKO15_001618"
     tRNA            complement(34637..34712)
                     /locus_tag="KKO15_001618"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34677..34679),aa:Ile,seq:cat)
     gene            34837..35343
                     /gene="mug"
                     /locus_tag="KKO15_001619"
                     /pseudo
     CDS             34837..35343
                     /gene="mug"
                     /locus_tag="KKO15_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8659 a         9044 c         9274 g         8366 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttacccaggg acagcaggag tatatcgagg tttatttttc agggaaaagt ctcggtaaat
      121 attatgcaat ggttaatctt gataccgtaa catttcttga tccagcaagt ctatataaca
      181 agctggaact ggatgtagac gatcagaaaa tcgcgcatat agtgaaagaa aaattatcgc
      241 agccgctagc tcgccacggt gaattggctt gcggttatgt acgtactgac tcagggtgtg
      301 gttttctgaa taccgatacg ctggaaataa tctataatga tgaagaaagt tcggcaacgt
      361 tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa gtcattatat ttaaatccag
      421 acaaaaatac tgttaacgct tttatacatc agcaagacat caatgttctg gcacaggatg
      481 attaccaatc gttgtctatt cagggaaacg gtgcgctggg aataacagaa aatagctata
      541 ttggtgcaca ctggaatttc aacggttatg atgcagatga tgtcagtgac agtaatactg
      601 acgtcagcga tctctattat cgttatgatt ttttacgtcg ttattatgtg caggcgggtc
      661 gcatggacaa ccgcacacta tttaatgcac aaggcgggaa ctttaccttt aactttctgc
      721 cactcggtgc aatcgatggg atgcgtatcg ggtcgacact gagctattta aaccaggcgc
      781 aaagccagca aggaacccca gtaatggttc tgctttcgcg caattctcgt gttgacgctt
      841 atcgtaatga gcaacttttg ggatcgtttt atctcaatag tggttcgcaa tttattgata
      901 ccagttcctt tccgccaggc agctacagcg tggcattaaa agtctacgaa aataaccaac
      961 tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc
     1021 aatggttctt acaggcaggt aaaactacat cacaggtttc tgatgatgaa agttcagctt
     1081 atcagctagg agtacgcctg ccattacatc cgcaatatga gctctacgca gggctggcga
     1141 atgccgatga tgtgagtgct ttcgagttag gtaataactg gacggcagat ttaggcaggg
     1201 tggggaatct tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc aaaggtgata
     1261 tgcaacaggc caactggagt aatccgggat ggccgacgtt gggcttttat cggaccaact
     1321 ctgacggcga tgcttgtaca accgacagca gagagagcta taacgcctta agctgttatg
     1381 aaagtatttc cgcgacagtt tcactgaatt ttgtcggctg gaatatgatg ctgggttata
     1441 cctgcacaca aaataacact gatgatagtt tgcgttggga taaacagcag agctttgaaa
     1501 ataactatct tcgccagaca acagcgcaaa gtatctctga aactgtacaa cttagcgctt
     1561 cccgcgctat tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt ttccatcgta
     1621 atgacaacgg tggcgataac gacgacaacg gcttgtactt atcgttttcg ttatctgaca
     1681 cgccaacgat ggatagcaat aacaacagcc attcaaccaa tgtgcctacg gattatcgtt
     1741 atagcgaaca ggatggcgat caaacgtcat ggcagttatc gcataccttt tataacgatt
     1801 cattcagcca taaagaactt ggcgtaaccg tcggaggcct gaacaccgat accataaaca
     1861 gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt ctacgctacc gtatctgata
     1921 gttatgaccg taagaatcat gatcatcttt cggcctttac gggcacttat agctctacgc
     1981 tggctgtcag tcgctatggc gttaatttgg gagccagtgg tacagacgat ttgctgggtg
     2041 cggtactggt ggatgtgaaa ggcttctctg aacaggatga agagagtcag gatctgcaac
     2101 tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt
     2161 tcccttatcc tggatttcag tctggttttg ttgaggttaa cgacagtagc cagggcaatc
     2221 agcaggggac aacaaacatc attaacggtg cagggaatcg tgaattaatg ttgttgcctg
     2281 gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta caactatatc ggtcgtttat
     2341 tactgccggc agcggtgaaa aaattcccga ttgtggggct gaacagcgcc atgttactgg
     2401 tagctgaaga tggcggattt acacttgaaa ttaacggcag cgaaaaagag ctctatctgc
     2461 tttccgggca gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc gccagcattc
     2521 gttacagcgg agatgttact tgtagtgtgg tgacttattc acaattaccg gagtcgattc
     2581 aggttcaggc acagttgaaa cagcctaaat tacgtggaaa cgttcagacg gcgcaaaggg
     2641 aggttgcacc atgagaaatc gattgattgc ggcgatattg ggcttgtttg gcacgctcac
     2701 tggcgttcag gcagctcctg acgtgaccag tgaaattacg tatgatttgg catctggtag
     2761 agcggattat tacttctgga aggatgaagc gtcggcagga aataatggat atatgtggta
     2821 tgaatgttcg tatcctgacc tccaacaaac ctgtacagct aatggaaata tatcgacagt
     2881 acaaatctat ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac tcaaaggatt
     2941 taaaacagcc attgtaagta gcgatgaagc gccgctagga tgcaaggggg gcaaagggct
     3001 tcagacgaat cttaaggatt ctaatagatc ttcatgtaca gaagatggtc aacattatta
     3061 tatatacgat acaaagtttc ttacgctcta ccttgagcag acagagatga agaatttgcc
     3121 gattggtggc gtctggaagg ggaaagttaa attacattcg aacagcccgg cccaggacta
     3181 tttcgcaaat attaccctga atacgctcga ccccaaccat attgacgtgt tcttcccgga
     3241 gttcgcccac gccacgccaa gggtgcagtt agacttgcat ccaacaggaa gcgttaacgg
     3301 cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg gttttaacgg
     3361 taatgccatc agttatgaaa tcatgctcaa agatgaaggg cgaccagctg cagggcgcag
     3421 agacggttac ttctctatct atcgtcaggg agggaccacc accgacgagg gagaacgcat
     3481 tgattaccgg gtcaaaatgt acaacccgga aaccggtggg caaattgatg tgcgcaataa
     3541 tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg tactgcccgg
     3601 tattcgctat gccgtgatgt gtgtgccgac acccttgact ctggcagtcg ataaattcag
     3661 cgtgatggat aaacaggccg ggtactacat gggcaaattg tcagtaatct ttacgccttc
     3721 cttgccaacc atcaattaac cctgcaaacc ggacaaacgc ttttttctat ataaacgacg
     3781 aagccgcctg ataaggcggc ttcgtcgtta taacttacgt taaacgttaa tgttgcggtg
     3841 cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg tttgttctgc aatacggtcc
     3901 agataaccag cgcacccagc aggtcaaata ctgccagaac tgcgaacagc gggctgaagc
     3961 cgatggtgtc agccagtgca ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg
     4021 acatcccggt taaaccgttc gccgtcgcca cttcgttacg accgaacacg tcagaagaga
     4081 gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc accgatacac agcagcataa
     4141 ttgcgacata cgggttggtg aacagaccga tcatacccgg gccaatcatc agcacagcac
     4201 ccagcgttac gaccatctta cgggaaacga tcaggttcac accaaaccaa cgctggaaca
     4261 gcggcggcag gtaaccaccg aggatacaac cgaggtcagc aaacagcatc ggcatccagg
     4321 cgaacatcgc gatctctttc aggttaaagc cgtaaacttt aaacatgaac agcgggatcc
     4381 acgcgttaaa agtaccccag gccggttctg caaggaaacg cggcagtgcg ataccccaga
     4441 actgacggtt acgcaggatc tgaccaacgg acattttctt cgccgtgctc acctggtgct
     4501 gggcttcctg accattaata atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc
     4561 gcggatgttt atagaaaatc agccatgcca tcgcccagat aaagctcaat gcaccggaga
     4621 tgataaatgc catctgccag ctgtgcatca cgattgccca taccaccagc ggcggcgcaa
     4681 tcatcgcacc aatcgaagaa cctacgttaa agtagcctac tgcgatggaa cgctctttcg
     4741 ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac
     4801 cgaccgcacc acgagcaaca gctaggccac cccagctacc tgccagcgcg gttgcaccac
     4861 agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca
     4921 gtacataacc tgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat
     4981 aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca
     5041 cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg atcatatacc
     5101 aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag
     5161 cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa
     5221 aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt ccgtaccggt
     5281 taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg acttatgatt
     5341 tgcgacggca gaaagataac ttgtcataca accttaaaag gtgagagcca tcacaaatgt
     5401 gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg cggcctgcag
     5461 cgagatttac cactttgaga gtaatttttt taactacgtt tattgatcta actcacgaaa
     5521 atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc ataagcaagc
     5581 tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag atttcctgtt
     5641 agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc cgattttcga
     5701 ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta aaaacctgta
     5761 tgacatctgg ttgaagggcg atcactacaa atggcgcgct atgcgtacca acggtgtggc
     5821 tgagcgtctg tgtactggtg atgcgtctga ccgtgaaaaa tttgacgcct gggcggcgac
     5881 tgttccgcac actatcggca acccgttata ccactggacg cacctcgaac tgcgtcgtcc
     5941 gttcggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct ggaacgaatg
     6001 taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc agatgaacgt
     6061 gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg cagagatcgc
     6121 caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca aagccttcaa
     6181 tatcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt ccgataccga
     6241 cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc acttcgccgc
     6301 tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg aagcgaacga
     6361 agcggaactg gacagcattc tggcgcgccg tctggctggc gaaaccctga gcgagcacga
     6421 agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg cacgtcgcgg
     6481 ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc agttcaaact
     6541 gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg aagagttgtc
     6601 taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc tctactgcct
     6661 gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg gcgaaggtat
     6721 gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag acggtatgga
     6781 acgtcagatg acccaactgg cgcagctcgg tctgctgagc cgctttgtcg gtatgctgac
     6841 tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca ttctgtgcca
     6901 gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc tgctgggcga
     6961 gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg aactgaacta
     7021 aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt cgcggtcgct
     7081 ttagcagatt tggctgaagg cacagaagtc agtgtcgata accagactgt tacgctgcgc
     7141 caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg ggccaacgtc
     7201 atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg ggaacacgtc
     7261 cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta tcaacctgat
     7321 tttcaggatc tgcctgcgca agcggcagat cctgaagtgc agatctatcg tcgcgctaac
     7381 ggcgatgtcg gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg tgtcaacggc
     7441 atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga aggtaccgac
     7501 ggcgtgttcc tcttcagcca cacctatggc tgctcacaac tgggcgacga tcacatcaac
     7561 acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt gctggtgatt
     7621 ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg cgatatcgat
     7681 cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga agccgggatc
     7741 gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc aggcaaactc
     7801 agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg tattactgct
     7861 aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac taccgtactg
     7921 accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg ccgcgacgaa
     7981 gcaacgtttg aaaaactggt caccatggtc aacgacttca aacagtactt tattgcccat
     8041 gatcagccga tctacgaaaa cccatcaccg gggaacaaag cgggcggtat caccacgctg
     8101 gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt tgacgtgctg
     8161 cgttacggcg agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc gggtaacgat
     8221 gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt cagtactggt
     8281 cgcggcacgc catacggtgg atttgtgccg acggtgaaaa tcgccaccaa cagtgaactg
     8341 gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca cggtaaagcg
     8401 atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa cggtaagcaa
     8461 acctgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg cgtaacgcta
     8521 taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc gccgtttgct
     8581 tttccatacc tgctaaggat ttatcatgac cgcgtattgg ctggcccagg gcgtgggtgt
     8641 catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc gcttcaaaaa
     8701 gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg gcacctatcc
     8761 cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac gcacgcgcag
     8821 cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg cgaagttcca
     8881 tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg cgctgttccg
     8941 ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct gggtgattca
     9001 caacttctgg gcggggtcga taggcggcac gatgattgag ggtagttttc tgcttatgaa
     9061 tggcctgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc cgtttaaagt
     9121 agagaaaacc cctcccgcca tagacgaaag gggttaaaca attaattacg cagggcgcta
     9181 tttgccagac gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac gtcagttgaa
     9241 gagttcagcg cggtttcgca agagtcctgc aatacgccga taataaagcc gacggcaacc
     9301 acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagtgg gatcagcagc
     9361 aaagaacccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac tacgctcaac
     9421 agcagcgctg tgggcagatc gaccggaata cccagcgtat taaccgcagc cagcgtcaac
     9481 acggtaatgg tgattgctgc gcccgtcata ttgatggtgg ctcccagcgg aatagaaact
     9541 gaataggtat cgcgatccag attcagcttt tcacacagcg ccatattcac cggaatgttc
     9601 gctgcagagc tgcgggtgaa gaaggcatac acaccgcttt cgcgcaggca cagcagcacc
     9661 agcgggaacg ggttacgacg aattttccac cacaccagca atgggttaac caccagcgcc
     9721 accagtaaca tacagccaac cagcacgacc agcagttgcg cgtagcccca cagtgtggag
     9781 aaaccggtgg ttgccagggt agaagaaacc agcccaaaaa taccgaacgg tgcgaagcga
     9841 atgaccagtt tcaccataaa ggtaacggca ttcgacatat cattgaccag gtttttggtg
     9901 gtctcgttac cgtgacgcag tgcgaagccg aggccgatcg cccacaccag aatcccgatg
     9961 tagttacctt tcagcagggc gtcgatgggg ttggaaacca tgctcattac cagtccgcgc
    10021 atcacttcga caatgcctga cggcggcgaa atatcacccg cgctactgga caagtgcagg
    10081 gtagaaggga aggcaaagct gaagactact gcggccagag ctgcagagaa ggtgcccagc
    10141 agatagagga acaaaatagg acggatattg gttttctgcc cgtgctggtg gttagcaata
    10201 gatgccatta ccagcatcaa caccaggatg ggggcaacag ctttcagtgc gccgacgaac
    10261 aaagtaccta acagaccaac agcttccgcc gcgggttttg agatccatgc cagaagaatc
    10321 cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag acgccggaat
    10381 agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttgt cgcacatccc
    10441 tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag gtcgagtata
    10501 aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagagg tcatgttttg
    10561 caacttaatg gttgcattta tttaacataa acagagggaa ggactggcta ctaaggctga
    10621 ttagataaca tatgggcaaa actatttgga atatgagaac ggttgcgctc ctttaacgtg
    10681 ttatgccata taaagtcact ggcgcaggga gcgcgcaggg ggcggccaat cgccgccgcc
    10741 ccctgcaccc ccaggctctg gcgaacaaaa tcgccgctgc gcggttccct cagcctatcc
    10801 cttgcggcta ccgggtcggg cgcaaggtaa cgtccctgta agatgcgccc tcgccccggc
    10861 ctccagggaa cgcttcggcg attttgacgc caccaacacc cgtgcggtct ttttgtaaag
    10921 agctataagc cgattacata attaatagct cacctttgtc tggaatgagt tatctctagg
    10981 aatggcttaa tatacagcga atcattctcc ccgctgctta tcatgccgat aattcaccca
    11041 ggcgttgata ataaacgtca tcaccagaat gccaaacacc acgcccagcg agacggcgat
    11101 tggaatatgg tagaagtcga caatcagcat cttgataccg ataaacacca gaatcaccgc
    11161 caggccatat ttgagcatcg agaaacgctc tgctacgccc gccagcagga aatacatcgc
    11221 acgcaggccg aggattgcaa acaggtttga ggtcagcaca atgaacgggt cagtggtcac
    11281 ggcgaagata gccggaatgc tatccacggc aaaaatcacg tcgctcaatt ccaccagaat
    11341 cagtaccagc atcagcggtg tggcgtacag caatccattc ttacgcacaa agaaatgctc
    11401 gttgtcgatg gtgtcggtca tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc
    11461 gccaatgcct gattcatctt catgggcgag cgccatcttc acgccggtaa acagcaggaa
    11521 ggcaccgaag atatacagga tccagtcgaa ctgcgaaatc agccagctgc ctgtgaagat
    11581 catgatggta cgcagaacaa tcgccccgag cacgccatac accagcacgc gacgttgtaa
    11641 tgccgccgga acggagaaat agctgaacaa catcagccag acaaagacgt tatcgaccgc
    11701 cagcgatttc tcaatcagat aacccgtgag aaaggccagg gcctgtggat cggcaacggc
    11761 gcgaccttcg gtttgcacca gataccacca gaaagcggcg ttaaacagta acgacagcgt
    11821 cacccagacc agcgaccagg ccgcagcctg tttcatggtc atggcatgtg ccccacgacg
    11881 cccctgcaac aacaggtcga tagccagcat aatggcgaca acaacagcga atccgcccca
    11941 tagcaacggc gtgccgacag tattcataga agttccttac acataaaaaa acggccaacg
    12001 tcggaagacg tcagccgctg ctttttatgc atagacctcg ccttccggca aggtctcact
    12061 tacaacaaaa aaggaatacg tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg
    12121 tttttcacac atcgtaatga cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac
    12181 aaagtatgac agaccattca gtcagtcaat ggtctgttac atacctttta cactgtttta
    12241 ctttggcaag tatagtttta cgctatctgc cggaaaaatc accccagttt ggcggcggat
    12301 ctcggtcagc agtttggctg tgatgcgact gaccgccagc cctggatgat ccaccagatg
    12361 ctcatccacc agggtagcga acagctctgc ttcgtagagc atggtattaa tatgctgcgg
    12421 ctgggtgaga tcctgcatct ggctgccacg cggcacaaag cagactttct ggcattcaga
    12481 cagtttttca atcaccagcg atcccgcttc gccctgaatc tcgcttccca ggacagaatc
    12541 actgaccttt gagtgctgca aggtgacgct gaaatcaccg taatccatca ccaccacgcc
    12601 gtgggcgtca acgccgcttg ccagcaaact cgccgttgcc tgcacgcttt tcggctcacc
    12661 aaataacgcc accgccgacg ccaggcagta aaagccgata tccataattg aaccgttaga
    12721 gaatgccgga ttaaaggtgt tgggattctc accgtccagg taacgttgat accgcgagga
    12781 atactggcaa tagttgaaaa agactttacg cagtttgcct gctttcggca gcgcctggcg
    12841 caacaaatga aagttcggca ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt
    12901 ttcgcgcgca caggcaatgg cggcatccac ttccgccaga ttcgacgcca gcggtttttc
    12961 gcaaatcaca tgaattttat ggctaaggaa aagttgtgtc tgggaaaagt gcagggaatt
    13021 cgggctggca atatacaccg cgtcaatggc atcgctttcc gccatcgctt ccagcgacgt
    13081 aaacagatgc tcgacagaaa aatcattggc gaagtgctga gcctgctcaa ggctgcggga
    13141 atatacggcg gttaacttgt atttaccgct ctcatgggcg gcctcgacga actggcgagt
    13201 gatccagttc gtaccaatca cagcgaaacg tatcataacg cgtggatact ccataaaggg
    13261 attctgtcgt caatgtagca cgcctttttc atcttcgacg tgcgcagata cgcattattc
    13321 tttcgccgga cagccatgct cactaaacgg cccaatcatg accgatggcc cgagatattt
    13381 cggctggcga tggaggattt ttgcgtcaat caccgcgctg acacgagcga gtttctcccg
    13441 cagttgagta taaaaaccgt gcttcatatt gaggtctggc gcgttaaacc cgatagcgtc
    13501 gatgccgtac tgttttgcca gccagatggc gcgttggtta tgaaattctt gcgagataat
    13561 ggtgatatgg ttttccccaa acaccttttt ggcacgcact accgaatcca gcgttgagaa
    13621 tccggcatag tcgcaaaaga tcactttcgc cggtacgcct ttagcgatca aagcctgctg
    13681 catacctgat gcttcatcgt aatttttacg tccgttatcg ccactcacca gtagccattt
    13741 cacttttccg gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt
    13801 acccggcctc gcccccagca ataaacccac gttacgcgcc gggacggcgt tgacatcgcc
    13861 ccaggtcagc tgcttgctgg cgttgaccat aacgcgatcg gcgataaaaa tggtcgctcc
    13921 tgccacaagt aacagtaagc aggcaatctt tagagtccgg cgagaaaaac aaatacggag
    13981 aagaaggcgg gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg gggaggtcga
    14041 ttttaggcgc taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc
    14101 caagcgacaa ctgcgttagc cacagccggg tgtcgaactc aagctggtgg tactgcggct
    14161 ccatatggca gcacaattga tagaacgctt tgttatgctc tttctccttc aggtgcgcca
    14221 gctcgtgcac cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga
    14281 cgcggatctc tgcttttgct ttcagcttgc cgccctgcac acgagaaaca gcggtatgta
    14341 acccgagcgc gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg
    14401 gggcattacg cagaaactga tttttcagat cctgagtata ttgccagaga gctttatcgg
    14461 tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt
    14521 taatcagtgt tcgcacctgc gaaagcagct gctcgggata accctgaaga taagtaagat
    14581 tgctcataac cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc
    14641 aacgtcgaaa ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc
    14701 actgacacta caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc
    14761 ggcagatgaa tatttgctgc aacagttgga cgacacagaa atccccggcc cggtgttgat
    14821 cctgaatgat gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat
    14881 tggcgactca tacatcagtg aactggcgac gcgcgagaat ttacgcctaa acgggattga
    14941 tgagtcgagc gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt
    15001 gctgatcaaa gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa
    15061 agtggtcacg tcggatacac gtattattgc cggtgccaaa gcccgtgaca ttcacacttc
    15121 cacgctggaa ctgttcgaaa aagtgctcgg cccgaccacc accacgctgg catggaaaaa
    15181 agcgcgcctg attaattgca cgttcaatga accgccgctg gccgatgcgc cgcagaccgt
    15241 tagctggaag ctggaaggta ctgactggac tatccataac catgcgaatg tcttctcccg
    15301 caccgggctt gatatcggcg cgcgcttctt tatgcaacat ctgccagaga atctcgaagg
    15361 ggagattgtc gatctcggtt gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa
    15421 cccgcaggcg aaagtggtgt ttgtcgatga atcgccgatg gcggttgctt ccagccgttt
    15481 gaacgttgaa accaacatgc cagaggcgtt ggatcgcagc gagtttatga tcaacaacgc
    15541 gctctccggc gtggagcctt tccgctttaa tgctgtgctc tgcaaccctc cgtttcacca
    15601 acaacatgcg ctgaccgata acgtcgcctg ggagatgttc cactacgccc gccgctgcct
    15661 gaaaatcaac ggcgagctgt atatcgttgc caaccgccat ctggattact tccataaact
    15721 gaagaaaatt ttcggcaact gcaccactat tgcgacgaat aataaatttg tggtgctgaa
    15781 agcggtgaag ctgggacgtc gtcggtaaga ttgggagtta cctgccggat gcggcgtaaa
    15841 cgccttatcc ggcctacgtt cagcacgtta cccccaggcc ggataagacg cggcaagcgt
    15901 cgcatccggc aatgtctgct cagttctgga atcgcgcttc cataaaacaa aaaatccctg
    15961 tcaccatcct ctacattctc ttgtttagcg tttttctacg tttattcttc cgtcacacag
    16021 ataaattcat ccgttgccaa tctgtcaacg gatgttatca tattgccaat ttgtcaacgg
    16081 acgtgatgaa gaatgcacct gataactcaa aaagcattga aagatgctgc ggaaaaatac
    16141 ccgcaacata aaacggagtt ggtggctctg gggaacacga ttgctaaggg atatttcaaa
    16201 aaacctgagt cattaaaagc agtattccca tctctggata acttcaaata tctggataag
    16261 cattatgttt tcaatgttgg gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa
    16321 tcgcaaaagt gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct
    16381 gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa
    16441 agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg
    16501 cgctggaact ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg
    16561 tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca
    16621 tggctcaagc catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt
    16681 taaccctttc cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg
    16741 ggaagaggaa attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc
    16801 ccgccttgtt tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa
    16861 cgatttaaat ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct
    16921 ccatagccac atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt
    16981 gcgccagcgc gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata
    17041 cctgcgtttc gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta
    17101 cgcctgggat cattttcaca ccccgcgaga gcaggatggt gcgatggatc cagccggtgg
    17161 ttttgcctaa cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg
    17221 ggctacgcgg gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt
    17281 cgatccccca ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac
    17341 ttaaatacat cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc
    17401 ctaccggcgc tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc
    17461 cgtcgatagg cggaacacgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct
    17521 gtaactgatc cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca
    17581 tccggcggta gtagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga
    17641 tattaaactg cccgccgatc tcgctgtgcg catcaaacaa tgttacctga tgcccacgcg
    17701 ccgccgcgtt aatggcaaac gccagcccag caggtccagc gccgaccacc gccagatttt
    17761 ttttctgcac ggcgggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca
    17821 ggcacgaggt gactttgcca acgaagatct gatcgaggca gctctggttg cagccaatac
    17881 aagtgttgat ctcatcggct cgccctgatt gcgcttttga cagcagttcc gcatccgcga
    17941 gaaacggtcg cgccatcgat actatatcgg catcgccgcg cgagagaata tcgtcggcaa
    18001 cctgcggatc gttaatccgg ttggtggtta ccagcggcag cgagacgtgg cctttcagtt
    18061 tgcgcgtgac ccagctaaat gcgccgcgcg gcacgggcgt ggcgatggtc ggaatccgcg
    18121 cttcgtgcca gcctattccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg
    18181 ccagttctac cgtttcggca aaagtgccgc cgccttcgac caggtcgagc atcgacaacc
    18241 ggtaaataat aatgaagtcg ttgccgacgc gttcgcgcac cgtacgcact acttctacgg
    18301 caaatcgcat ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca
    18361 gcgtcagaaa ttcgttgatc aaataccctt cagagcccat cacttcgacg ccatcgtatc
    18421 cggcctcccg cgccagttgc gcgcagcggg cgaaatcgtc gatcagttgc aggatctctt
    18481 catgggtgag ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gacggtgcga
    18541 ccagatgcgg ttggtagctg tagcgcccgg tatgcaaaat ttgcagggcg attttgccgc
    18601 cttcctgatg tacggcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga
    18661 gcattgcgct accttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa
    18721 tcagcgccac gccgtgacgg gcgcgttcgg cataaaacgc agccagccgt tcagcaccgt
    18781 ccgggtattc ctccagcccg gtgtgcattg aacccatcaa cacgcggttt tttaacgtgg
    18841 taaaacctaa atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt
    18901 aaaataattg ttatgtagtc ggatgagttc taatttagcc gtcgatggag aaaagggaaa
    18961 aggggagtgc ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat
    19021 gcgacgcttg ccgtgtttta tccggcttgg gggattgcac atgtagggcg gataaggcgt
    19081 tcacgccgca tccgccagcc gttgcaaatt actgcttacg gaaaaaatcg ttatacaaca
    19141 tatacaaatg agcggcactc caggagaaat ttggtgcgcc ttgctgcgcg cctgtcagcg
    19201 ggttgtaatt ctcctgaatt gggccatcgg cggttaatcc tttggcgtgc ctgaagaacg
    19261 tatccgccag cttcagggca tcatcacgat aaccgtaacg ctccatccct ttcagaccaa
    19321 accagaactg atccacccat acgcgcccgc gccagtagat atcagcgcca aaagccggat
    19381 tggttaacgc cgccgttccc agcggaacaa aggtgttgaa ctctttagga tcgagcatca
    19441 ccttcaccac cgcgtcggca ttggcctgcg ttgccgcacc gttaaacagc ggcgaccagc
    19501 cttccggccc tttaccgcgc tcaacaatcg gtttgcccgc gcagccgttc gccagtggtt
    19561 tatcttcaat acgtacgtca tagtagaact gggtagtcgg gtcgaacata caggtgttga
    19621 tgtagtccgc gagctgctgt gccaactggc gatagcgttt ggcctcttct ggcttaccga
    19681 ggatggttgc catctccgcc agataatggt tatcgctgta catatagctg gcctgatcca
    19741 ccgactcctg caatagcgag tagcccagca acgttccgtc ctgactgcgg ttttcggcga
    19801 atttcaccgt ccagtcgcta cgtttgccgc cattagcgac atatttatcc aactgttctt
    19861 tgtcgataaa accaaagacg gcggcatcat cacggcccga ttcccacgac gcagcgacct
    19921 gtgccggaat ttccagactg tcgtactggc ctttctccac cacgcgggcg tagttgttca
    19981 gcccagactg cgtctcttct ttatcgcctt ttttcaccgt aaacagcatc tcgccgctct
    20041 cggtgttgtg ggctttgtcg cgggtcgcgc catattccgg caccccgttg ccgttatgat
    20101 cgcggttacg taaccaccag tcgtgatagg ccaccagttt cgggtacatc tctgccaacc
    20161 aggctttatc ctgggtgacg ttgtatactt ccatcaccga ccaggcggca aggctgggtt
    20221 tggtattacg ttcgttccag ttgccgccat cgccaccacg ctcggggcta aggttccacg
    20281 ctatcaggtc ggggacaaag cccacatcct gcggacgcac gctgtcgcca ggctggatct
    20341 gccaggagaa gaccgcgcgg atattctctt tggcgatatc cggattgaaa tgcgccatcg
    20401 caaacgcctg cttccaggta tcccacggcc aggtctgatt gccggagaac cagcgtccgg
    20461 tcaccgacgg cgtgacggtg ttataattca ccgcaccgcc cggtgagcgc cagttaccgt
    20521 tgagcgtttc gatcgctttc accgcgacgc gcgtctgttc cggcgtcgca tccggattgg
    20581 ttaacccttt cttcaggtat tcttcccagc gttgctgcga ggcggtgaga taaaacgccg
    20641 gacgcgccag aatatcgcgg atctgcattt gctctttgct aacttcctga gcggtcagca
    20701 gatgggaata ggtggtgtag agcgtggtcg aaccgttgat atgcgcctta ctggtaaagc
    20761 gattaccgtt gatttcagtc tgcaccggca gggatttatg cacctgatat tccgattcgc
    20821 cggaggtcag cagatcccag gtggcgcgca ctttgccaaa ggtgactttc aggccatcac
    20881 gggtggcgct gattttgcgc tgatagtcag ggtattcgcc ggcaatggtt ttatcggaaa
    20941 gcggtttccc ttctttcgct tccagttttt ccagcagttc gccatcccac accagatcca
    21001 gcggtttatt gctgatgatt ttagtttcca gtagtgacgt gcgcggcgtg gcgaagcgca
    21061 gagtcatttc gacctgcaca tcttttgctg tcagtttttg caccagcgcg ccgggaatac
    21121 tgtatgcctc cagcgtgaag ttgactttct tgccgtcttg ccagacagtc aggcggtcga
    21181 aattgctggc cataaagttg atgtattctt ccgtcagcag cgcaacgccc ggaaagccgc
    21241 ccatggtgtt cgggccgtct ggcaacagat gaccatgcca ggctccgaga tcgaaaaacg
    21301 gattaaaacg ctggtgatcg tcgtaatcgt aatccttcat gtactgcggc gcaccagtac
    21361 ggttaatcac gtttttataa ttgtcggcgt tagcggcatg agcggaaaaa cttatcagca
    21421 gagcgcaggt tactggcgtt aaaatagttt tgattttcat gagattacag tccttattac
    21481 cagtagaaat attgcatcag gaagatttgg tcgctgggat cgttagaggc atttttcatg
    21541 tagaagcggc tatcaaaagc cgtggcgaaa cgtccgccgc catattcgta ccagatacca
    21601 aactgcatga aagaggtggt ttcatcgtca gcgtggtcag ggcgatgttt ggcgtaggtg
    21661 taagcggtgg agagataaac gccttttaac gcttcataca tggcgctcac catcatcccg
    21721 ctttcacttc ccggtacgtc cagcccgtcg gcacgtccgg tatggtgcca ggcgcgagcg
    21781 gcaaagttag gggtaaacag gcccgtgaga tagagttgtc ctgtgccgtc ggtaatttcc
    21841 agaccattca tccaggtgat gccttcggca aggtcatact ggatgtaacc gttcatccat
    21901 tgccggatag gtgtatttgt cgtcgtagcg atcgtacttg ccaaaatgta gccaggcttt
    21961 gctttcgtca tgatgtccgg caggtgtggc ggtgacgcta taacgaaagt tgccgcttaa
    22021 gttctgtacc ttcagcgcgg tcataatgtc gcgggtattg ggaatgacgt agcccaaatc
    22081 gggtgtgaag tcgccccacc attgcaggtc atccagcgag gaatcctgac gcagactgag
    22141 catgatttcg gtgccatcat tggtacgata accgccatag aaacggttaa tcccaccttc
    22201 gaaaccaccc cagctatggt caggcaccta ggcattaccg tcgtggtcgg cctgcacggt
    22261 ccagccttcg ccgtaaatca ggccgaacca ggagccgtac ttcatctcca ggccaccttc
    22321 aatataggtg ccgtcgctgt atttatgttt attctcaccc tctaaatcca tatatccacc
    22381 aagccctaat tcgccgtaaa aacgaaatga aggagaagat gtcgaggtaa ttgcgggtgt
    22441 ttttgtgtcg tcatgttctg ccggacgtgc ttcttctcct gccgcagaaa aggcgtagca
    22501 aaacggcaga gcaagtaatg ctctgcatgg tgcagtgatt aatttcattg tgttaatcca
    22561 tgagctaaag aaaaagtaca gccttttatc gttgtaatta taaaaatata aaggcagaaa
    22621 ctattttccc tctcctcatc caggaagagg gaaaatgcct gctcattttt tattagtctg
    22681 cttaatcaac attgctggct ggcgtcgctt caatatggcg ctcttcagcc gttaatccct
    22741 ttatatattt gctgtatttc caccacgcaa agccgaggaa gataacaata ccgccgacgt
    22801 tataaaaaat gatggtcaga atattcgcgc cagtcgggaa tgtcgaagcg acaaacccta
    22861 cggcaaatat cgcaatcagc attgaaacaa cgataatacc ggtgcggcgg gagcccatac
    22921 ggaaatcgcg tggcaggtga tctaatttgg cgcgtaaatt caggtaagcc agcatgatga
    22981 ataacggcgg aagcatggac gctgcggcgg tcatattaat aatggtattc atcagatcct
    23041 gcacggtatt ggaaccgagc atcgggataa tcatcagcgg gatgacgatc agaaactgga
    23101 tccatgctgc gcgcgccgga acgccgtttt cgttaagttc gacggttttc ttaccaaaga
    23161 tgccttccgg gatttcggag aagaaaattt tcaccggcgt tgcggtccac atcagcaggg
    23221 aaccgaacat tgcggtaaag gacaccagac cgacaaagcg attcatcaac gcttccggca
    23281 gaccaaaata cgccgccatg ccgtggaata cctgcaccga tccgccggta aatttcaact
    23341 ctttgctgct gacgaagacg ttaatcagca ccgaggagac ggaatacagt acgccgataa
    23401 aaatcccggc gaggatgatc actttaacga acgatttcga accgcctttg acgtcgttaa
    23461 cgtacaccgc gacggactcc gcgccacctg cggccataaa gatccaggtg gtaacgccga
    23521 ggaacgccca gttgaagttc gggatcatcg cgtcaacggt gatcggatcg gcaggctgta
    23581 cgccgccaac cagtgccgta cctgccagta aaatgtagga gagcgttaac agcagcatca
    23641 gcgttgaagt gacggaggta attggcccca gcattttcgc cccgttggtg gaaacccagg
    23701 tggagaaggc gaacagcacc atactgataa cggtggtggc aaccggcgtc ataatatatt
    23761 cgtagccgag gaaggcatac gaagcataag caataacgcg cggcaacagc gaggtgaaaa
    23821 agaacagatt tacgaaccag taagtgtagg cagtaataaa tgcccaacgt ccgcccagcg
    23881 aactttttac ccacgcgtag acaccggctt ctgagttttt atttaacgaa acaaattctg
    23941 cgatgatcag acagaaggga ataaaataaa aaatcgtcgc gaggaaaaac atcggtgccg
    24001 aggccagtcc aagctcaata ttattattga taacgttgtt aaagctgtaa acggcggcaa
    24061 aagtcagcga gagcaagccg aatttgccga ttgtattacg tttggtatca gacataacac
    24121 atctccgaaa atgtgtaggg tagcggtggc ggcaaactcc ttttgccgcc gtagttgtta
    24181 tttgttatgg aaataaccat cttcgatggt gactttgaga accacttttt tgaccgcgtt
    24241 attgcagata aaccgatacg cttcatggat atcgcaaata acgatttgcc cttcgtggac
    24301 ctctacggtt tctccgcagc cttttaaata ttcacggtca gtttcatcac gataatattc
    24361 cactacctgt aatgtctctt tcggcgcata ttcaattttt tgctgccctt gcaggtagta
    24421 atgcacttca aaatagcgtc gatgcccggt aaatagcgca tcggtcgcgg agtcattttc
    24481 cacacggtag gtcaatgaat caccgatgga gtgggcgacg ccaggctgaa tgttgtcgat
    24541 attttcaatc gcttcaacgc agcgttgcca cttcttgcca gaggcgtaaa tctggcggaa
    24601 ctgttctaag ttatcgatga tcctcattgc ttattctcgc tgtgcaaatt cgtggaaaag
    24661 aaccctgcgc cgaactcata cgacgccagg ctttgcgcgg tagcttctcc gccagaaacc
    24721 ggcagcaacg taaagccgta gctgaagtca cggaaccaga cgcgccagga gtccagcacc
    24781 tcgctgcccc aggagttgga gccgaggcca agtagctggt ggtcgaggtt cagggtgatg
    24841 tcatcactgc gctgcagctc gttacagtgc tgggcagcgt ggatgttttc ctgggtatag
    24901 tgccaggcgc tgaagttaat tgggcgctgc ggaaccacca gcagaccgtt gccgtggcgg
    24961 ttagtcagtg ccgtccagcg gacatgctga cggttgccgt tgttctgcgg gaagggatag
    25021 ttctcgaaca tggcatcgac ggtgctgcgc cagatatcga tgatgttagc ctgctggctg
    25081 tcggcgtagt tttcgcccgg tccacgaccg taatacgcca cctgatcgta ttcgccgtta
    25141 atccccatgg tgaaaccgat gcacggaatg atgtgcggat agtcgccgta acgctcgccg
    25201 gaaagcgcca cgttaacctg gccatcggca gcgatgcgcc agatgtaggt acagcgcatc
    25261 ccgaagtcaa acaccggcgg tgcaataact gtgcggctga tgatcaacac ttcaccatcg
    25321 ctctgttcta cggcaaagtc gcgcagatgt tcctgcatga tctgcaaatg attcggctgc
    25381 cacaaccctt cgtactcctg cttgtggttg tcgatcatcg gcttgaagaa gttgatcttt
    25441 ggctcgcggg tcagcagcga ctcgccattt acctggcagg atgtcggttt gccactcatt
    25501 tttgagaagg tgatcgcgaa gttgtagccg cgaacggtgc agctcaaacg atcgtcttcc
    25561 agcgtcagcg gacgcgcatt atttggtgcg aaaggcactg gctgcgcggt gttttccttc
    25621 agcgggaact gataagtggc gatagaatgc ccggcttcgc tgtagcgggt gcgggaatct
    25681 ttggtcaccg taatgttgag gaacgcttcg cgggcgtcca gctgcggcag cgtgatctgc
    25741 aagggggctt cgctgttcgg cgcaacgtcg cgcagtttaa tctgctgcgt cgcgagcgtt
    25801 tcaccttcgg cgcgcacctc tgcgtgcagg gtgtagtcat caagcgtggt aaaccacagt
    25861 ttattttcga ctttcaactc gccgcgagtc agcgcgtgga tttttaccgg cgcgataacc
    25921 tgtttgtact ctttcaggcc cgatcccggc gtctgatcgg aatagatcaa accatcaaga
    25981 cagaagttat agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg
    26041 ccgtggtcgt cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga
    26101 atgcaatcgt gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc
    26161 atcgcatgag catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca
    26221 ttcatcagcg gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga
    26281 tcttcttcgt aatgaaccag tcgcgtgtca tccagcgctt tcgccgcatg gtacatcgcg
    26341 cggatgttac agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg
    26401 tttttctgtg cgtggatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga
    26461 tcgtcggtaa tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt
    26521 tccgccatca caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg
    26581 tagtgagcgg tacgcacgga gttgatattg tgctgcttca tcaactggag atctttctcg
    26641 acgcgatcca ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg
    26701 tgcagcatca cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg
    26761 aagccaacgc gttgtggcac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg
    26821 accagatggt aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa
    26881 gcaaagctgg cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc
    26941 acgcgttcgc catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga
    27001 ttttccagca ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta
    27061 cgcacagtga aatcgttaat atgcgttagg tgttttccga ccagataaac atcgcggaag
    27121 atccccgccg accaccacat atcctggtct tccacgtagg tagagtccgc ccactgcatc
    27181 atgcgcacac acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg
    27241 gtcaggcgac tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag
    27301 gtttcgacgc cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg
    27361 aaaatacgtt gataggcacc ggttgggtta tcgctgggga caaacggcac atcgatgggg
    27421 aacggaaaac cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg
    27481 gggacggtaa tatgccccca gtcagccatt aactcagagg tgaaggcttc tggtacttgc
    27541 agcggatggt caaaaaagtg gaaattccac tgactgctta agggcagaaa caggctgctg
    27601 gtttcgcggg caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgc
    27661 ggcgcaagtc ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc
    27721 ggtagagaga aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggggtta
    27781 gtaaaatttt tactaaaaag cagcaggtaa atgcatttac tttaactgga tcacgaaaat
    27841 aaacggcagc actgtccggc aaaaatgatt gccggacagt gtgtaagggg atttaacggg
    27901 tcgtgccgcg cagttttaat ttgctgggaa cgaagactag cagcggcagc gcgcgaccat
    27961 cgcgggcttt ttcatacacc aggttaacgc cctgacttcc catcatctcg gaatggatgc
    28021 gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg gatatcgtta acgctgataa
    28081 gcgaaatatc ttgtgggatg ttcaggccac gttcatgaat tgcccgcagt acgccgatag
    28141 caatggaatc ggaagcaaca aacagtgcct tcggatagtc ttcccgcgcc agcatttgtt
    28201 ttgccagttc ataacccgac gaactggaaa aaccgccgcg ccagatatcc tcttcgcgta
    28261 ccacttgttt cagtcggcca tattccgcaa aggcgacctc acgaatatcc gccttgccag
    28321 gctcatcttc accgccaata aaaccaatac gattaacgcc ctggttgata tagaagtcga
    28381 tgatttcttt actgatgcgt gccagatcga tatccaccgc atcgtaaccg ctgccgggtt
    28441 cgtgaaagtc gataaaacag atattgtcgg tcaacgcgct ggcagcggcg cgcagggcgg
    28501 gcgtgggttt gccgacaatt aaaataccgg tgacgttttt aatgtctggt aatccgttgt
    28561 gttcataaca gttggtcagt tcgatcgcca gcttttcgca ctgagtttca atgccgtggc
    28621 ggatcgccag atagtaagga tcgttgatct ccagctcctg ctggtagctg tagatagcca
    28681 gaatatggtg ttggttgact gcgcctgtct gtagtttacg ggcgctactg gtcttgtact
    28741 ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc ttctttcaca ttcaatgtcg
    28801 gatcgtcatt taagaccctg gatactgtcg ccagggatac gccagcttcg attgcgatgt
    28861 cttttaatgt tgccatgttt acctttttta ctttccctga tgtgcgcccc cattgtagag
    28921 gaagtgctta tcgggtcacc tgttttcagt aaataaagcg cgtttctttg acgctgtaac
    28981 ggtaacacag cggcttagga atgtgatcac gcctgcattc tccattttta gcctacagga
    29041 ttgagaaaaa cttttctata gtttagtaaa attttacccg aagggaggaa gcaatggaaa
    29101 ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga
    29161 aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg aaaaaacact
    29221 gcaaaccgtg accagcctgg tgccgtacta cagcgaagag gagctgatgg ggaaaacggt
    29281 ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct
    29341 gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg agcggatgat
    29401 gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc gccatccgat
    29461 ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac gcgccgcttt
    29521 gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt
    29581 ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt
    29641 atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac cacgcgcttc
    29701 ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca acatatcgcc
    29761 tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg tcgccagcgc
    29821 cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg ggttgtcgaa
    29881 cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca cgccgccgcc
    29941 gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc aggcaaacat
    30001 cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg cgccgaactc
    30061 atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta cgccaccttc
    30121 gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt tgcactcatt
    30181 aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc ctggcagcag
    30241 tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc caagtgattt
    30301 accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg actgataagc
    30361 cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga agaagctgta
    30421 tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc gtaacggatc
    30481 gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg cggaaaccac
    30541 aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt cgataaactc
    30601 ctgtccctgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta cggctccgta
    30661 atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga catgctcttt
    30721 gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca gcgttcgctt
    30781 ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg aaggtaacct
    30841 gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt attgcaggga
    30901 ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg ttatcatttg
    30961 tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt ttgtcgggta
    31021 tggatttgat gattgctgcg caaatgcacg ttttcagggc aataattgcc tgatttttgg
    31081 gcaagataat taacgttttc gtatagtaac agagtgaaag tattttaaat caaagaatta
    31141 gatttgtgct ttaatcgcaa attgcgatct aaatcaaatt aatcggttaa agataaccgc
    31201 agcggggccg acataaactc tgacaagaag ttaacaacca tataacctgc acaggacgcg
    31261 aacatgtctt ctcatccgta tgtcacccag caaaataccc cgctggcgga cgataccact
    31321 ctgatgtcca ctaccgatct gcaaagctat atcactcatg ctaatgacac ttttgtgcag
    31381 gtgagcggct ttaccttgca agagttacaa gggcagccgc acaacatggt gcgtcacccg
    31441 gatatgccaa aagcggcgtt tgcggatatg tggttcaccc tgaaaaaagg ggagccctgg
    31501 agcggcatcg tgaaaaatcg ccgcaaaaat ggcgaccatt attgggtgcg ggccaatgcg
    31561 gtaccgatgg tgcgcgaggg aaaaatcagt ggctatatgt cgattcgtac ccgggcgacg
    31621 gatgaagaga ttgcggcggt ggagccgctg tacaaagcgt tgaactccgg acgtaccagt
    31681 aagcgtattc ataaaggcct ggtggtgcgt aaaggctggc tgggtaaact gccttcatta
    31741 ccgcttcgct ggcggacgcg tggagtgatg accctgatgt ttatcttgct ggcggccatg
    31801 ctttggtttg ttgctgcccc ggtggtgacg tatttcctct gtgtgttagt ggtattgttg
    31861 gcaagcgctt gttttgaatg gcagattgtg cgcccgatag aaaatgtcgc ccgtcaggca
    31921 ctgaaggtgg cgaccggaga gcgtaatagt gttgagcacc tgaatcgcag cgatgagctg
    31981 gggctgacat tacgcgcggt agggcagctt ggcctgatgt gccgttggct aattaacgat
    32041 gtctcaagcc aggtgtccag tgtcagaaat ggcagtgaga cgctggcgaa aggcaccgat
    32101 gaactgaacg aacataccca gcagacagtt gataacgttc agcaaacggt ggcgaccatg
    32161 aaccaaatgg cggcgtcggt gaaacagaac tctgccacgg cgtcggctgc cgataaactt
    32221 tctatcaccg ccagtaatgc ggcagtgcag ggtggggagg cgatgaccac ggtgatcaag
    32281 acaatggacg atatcgccga cagtacctag cgcattggca ccattacttc gctgattaac
    32341 gatattgcgt ttcagaccaa tattctggcc ctgaatgcgg cggtggaagc ggcgcgtgcc
    32401 ggcgaacagg gcaaaggttt tgcagtggtg gcaggggaag tgcgtcattt agccagccgc
    32461 agcgctaatg ctgccaacga tattcgcaag ctgattgatg ccagtgctga taaggtgcaa
    32521 tccggttcgc agcaggtaca cgccgccgga cggacgatgg aagatattgt ggcacaggtg
    32581 aaaaacgtca cccagttgat cgcccagatt agccattcaa cgctggaaca ggccgatggg
    32641 ctttccagcc tgacccgtgc agtggatgag cttaacctga tcacccagaa aaatgccgag
    32701 ctggtggaag agagtgcgca ggtgtcggcg atggtgaaac accgcgccag ccgactggaa
    32761 gacgcggtga cggtgctgca ttaatcggta tgccggatcc ggcggttgga gcacaatgcc
    32821 tgatgcgatg ctggcgcatc ttatcaggcc tgcggggtgt gtagcgggtg taggccggat
    32881 aaggcgcgtc aagcgtcgca tccggcaatt gcaccgcgcc actggcggat gcggcgatta
    32941 atccagttgc gtaatatcaa aagcggcgcg gtcgatcacc gcaatgatct tttttatttg
    33001 tgcatcactg atatcgctct ggttgacgcg taaatccagc accgctttaa aattatccag
    33061 cgcccgcttc atttgcgggt tctggcgcag cgccgcgcca acgcaacgcg ctttgatgcg
    33121 ttcttcaatc atctccactt gttcgcggtt ttcttccagc cactgcgcgc cttgttcggt
    33181 cagcgcaatc tgcttcttac caccttcctc ttcgcggatg gtaatcagcg actgctcctg
    33241 cagaaaatcc agcgtcgggt agatgacgcc cgggcttggg gtgtaattcc cctgggttag
    33301 attctcaatc gctttaatca attcgtaacc gtggctgtca tcgcgcgaga gaatatccag
    33361 aatcaccaga cgcaattcac cgtgaccaaa gaaacgttgc cgacgaccgc cgccgcgacc
    33421 gtggcgacca ccgcaacatt gaccgtgttc atgctggtga ccgtgtccgc agtgctcgtg
    33481 ttctgacttc tcacctttgc agcagccctc atggcgtggc tggccttcat gtttacaaca
    33541 cccttcgtga tgatggctca tatcagcctc cttttgcttg attagatata tctaatttat
    33601 atctgatgtg agcattactg caagtacaaa aatgattttt aaacgtggaa gcacctcaca
    33661 tctaggccgg ataagacgtt cacgccgcat ccggcagcat tgctatggca ctgaatgcct
    33721 tcacatactt cagacatatc agttctacaa atcgcttgca tttatcatga ttaacaatca
    33781 ttatcatttg cgaattttat ttagatatat ctgattacca tcacgaaggc gataacaatg
    33841 aataacaccc cccgctaccc gcagcgcgtt cgcaatgatc tgcgcttccg tgaactgact
    33901 gtgctacgcg ttgagcgcat cagcgccggt tttcagcgca ttgtccttgg cggcgaggcg
    33961 ttggacggtt ttacatcgcg cggctttgac gatcacagca aactcttctt tcctcaacct
    34021 gacgctcact ttgtgccgcc aacggtaacg gaagagagca tcgtctggcc ggaaggacca
    34081 cgcccaccgt cgcgtgacta tacgccactg tatgacgaac tacgccatga actggcgatc
    34141 gatttcttta ttcacgacgg tggggtcgcc agcggctggg cgataaactt acggtggcag
    34201 gtccgcgcgg ttcgctggtg gtgccggaag attacgcgta tcagctgtat gtctgcgatg
    34261 aatccggaat gcccgcactg cgccgccgcc tggaaacgtt gagcaaactt gccgttaaac
    34321 cgcaagttag tgcgctggtt agcgtgcggg ataacgcctg tcaggattat ctcgcgcacc
    34381 ttgatggttt taatatcgaa tggctggcac atgatgagca ggcggtagat gcgcgtctgg
    34441 cgcagatgca aatccctgcc gatgattact tcatctggat aaccggcgaa ggtaaagtcg
    34501 ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg gtacgtgcag
    34561 cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac gatttctcgt
    34621 cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa gcgattatga
    34681 gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa gtaactccgt
    34741 gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat tttcaatccg
    34801 ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat tttggctcca
    34861 gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg gactggtttt
    34921 ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg gtttaccgac
    34981 cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg catcaccaaa
    35041 ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct gcacgcaggt
    35101 gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc gattctgggc
    35161 aaacaagcat atgaacaggg attcagctag cgcggtgcac agtgggggaa acaaacgctc
    35221 accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca
    35281 ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt gcgtgggcga
    35341 taa
//
LOCUS       DAECOD010000032        34918 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.032, whole genome shotgun sequence.
ACCESSION   DAECOD010000032 DAECOD010000000
VERSION     DAECOD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.032"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KKO15_001620"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KKO15_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBD6360440.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KKO15_001621"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KKO15_001621"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBD6360441.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KKO15_001622"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KKO15_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBD6360442.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KKO15_001623"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KKO15_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBD6360443.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5658..5900
                     /locus_tag="KKO15_001624"
                     /pseudo
     CDS             5658..5900
                     /locus_tag="KKO15_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5942..7018)
                     /gene="glpQ"
                     /locus_tag="KKO15_001625"
     CDS             complement(5942..7018)
                     /gene="glpQ"
                     /locus_tag="KKO15_001625"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD6360444.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7023..8381)
                     /gene="glpT"
                     /locus_tag="KKO15_001626"
     CDS             complement(7023..8381)
                     /gene="glpT"
                     /locus_tag="KKO15_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBD6360445.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8654..10282
                     /gene="glpA"
                     /locus_tag="KKO15_001627"
     CDS             8654..10282
                     /gene="glpA"
                     /locus_tag="KKO15_001627"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBD6360446.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10272..11531
                     /gene="glpB"
                     /locus_tag="KKO15_001628"
     CDS             10272..11531
                     /gene="glpB"
                     /locus_tag="KKO15_001628"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBD6360447.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLIDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11528..12718
                     /gene="glpC"
                     /locus_tag="KKO15_001629"
     CDS             11528..12718
                     /gene="glpC"
                     /locus_tag="KKO15_001629"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBD6360448.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12911..13861
                     /locus_tag="KKO15_001630"
     CDS             12911..13861
                     /locus_tag="KKO15_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD6360449.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13902..14372)
                     /locus_tag="KKO15_001631"
     CDS             complement(13902..14372)
                     /locus_tag="KKO15_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD6360450.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14427..15629)
                     /locus_tag="KKO15_001632"
     CDS             complement(14427..15629)
                     /locus_tag="KKO15_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBD6360451.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15716..16258)
                     /locus_tag="KKO15_001633"
     CDS             complement(15716..16258)
                     /locus_tag="KKO15_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360452.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16537..16962
                     /gene="nudI"
                     /locus_tag="KKO15_001634"
     CDS             16537..16962
                     /gene="nudI"
                     /locus_tag="KKO15_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBD6360453.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(17001..17603)
                     /locus_tag="KKO15_001635"
     CDS             complement(17001..17603)
                     /locus_tag="KKO15_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD6360454.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17911..19050
                     /gene="arnB"
                     /locus_tag="KKO15_001636"
     CDS             17911..19050
                     /gene="arnB"
                     /locus_tag="KKO15_001636"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBD6360455.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19054..20022
                     /gene="arnC"
                     /locus_tag="KKO15_001637"
     CDS             19054..20022
                     /gene="arnC"
                     /locus_tag="KKO15_001637"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBD6360456.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20022..22004
                     /gene="arnA"
                     /locus_tag="KKO15_001638"
     CDS             20022..22004
                     /gene="arnA"
                     /locus_tag="KKO15_001638"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBD6360457.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            22001..22891
                     /locus_tag="KKO15_001639"
     CDS             22001..22891
                     /locus_tag="KKO15_001639"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBD6360458.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22891..24543
                     /gene="arnT"
                     /locus_tag="KKO15_001640"
     CDS             22891..24543
                     /gene="arnT"
                     /locus_tag="KKO15_001640"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBD6360459.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24540..24875
                     /gene="arnE"
                     /locus_tag="KKO15_001641"
     CDS             24540..24875
                     /gene="arnE"
                     /locus_tag="KKO15_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBD6360460.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24875..25261
                     /gene="arnF"
                     /locus_tag="KKO15_001642"
     CDS             24875..25261
                     /gene="arnF"
                     /locus_tag="KKO15_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBD6360461.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25255..25527)
                     /gene="pmrD"
                     /locus_tag="KKO15_001643"
     CDS             complement(25255..25527)
                     /gene="pmrD"
                     /locus_tag="KKO15_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBD6360462.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25632..26987)
                     /gene="menE"
                     /locus_tag="KKO15_001644"
     CDS             complement(25632..26987)
                     /gene="menE"
                     /locus_tag="KKO15_001644"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBD6360463.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26984..27946)
                     /gene="menC"
                     /locus_tag="KKO15_001645"
     CDS             complement(26984..27946)
                     /gene="menC"
                     /locus_tag="KKO15_001645"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBD6360464.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27946..28803)
                     /gene="menB"
                     /locus_tag="KKO15_001646"
     CDS             complement(27946..28803)
                     /gene="menB"
                     /locus_tag="KKO15_001646"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBD6360465.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28818..29576)
                     /gene="menH"
                     /locus_tag="KKO15_001647"
     CDS             complement(28818..29576)
                     /gene="menH"
                     /locus_tag="KKO15_001647"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBD6360466.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29573..31243)
                     /gene="menD"
                     /locus_tag="KKO15_001648"
     CDS             complement(29573..31243)
                     /gene="menD"
                     /locus_tag="KKO15_001648"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBD6360467.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31332..32627)
                     /gene="menF"
                     /locus_tag="KKO15_001649"
     CDS             complement(31332..32627)
                     /gene="menF"
                     /locus_tag="KKO15_001649"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBD6360468.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32706..33011)
                     /gene="elaB"
                     /locus_tag="KKO15_001650"
     CDS             complement(32706..33011)
                     /gene="elaB"
                     /locus_tag="KKO15_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBD6360469.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33066..33527)
                     /locus_tag="KKO15_001651"
     CDS             complement(33066..33527)
                     /locus_tag="KKO15_001651"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6360470.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33592..34509
                     /gene="rbn"
                     /locus_tag="KKO15_001652"
     CDS             33592..34509
                     /gene="rbn"
                     /locus_tag="KKO15_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBD6360471.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34700..34888
                     /locus_tag="KKO15_001653"
                     /pseudo
     CDS             34700..34888
                     /locus_tag="KKO15_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8539 a         9347 c         8651 g         8381 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcgccgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtcggataca gcgatgaatt
     5701 ttattcgaca gggattaggc atcgccttac aaccagagtt aacgctgaaa agcattgcag
     5761 gtgaattgtg ttccgttcct cacgaaccaa ccttctatcg acagatttcg ttgctgacta
     5821 aagaaaagcc ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag
     5881 tggcgattgg aaaaatttga tatgagacag gatggcgcat gaacgccatc ctgcagtaac
     5941 attactcttt attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta
     6001 cacccgcttt gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca
     6061 gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctgg gcatcttgca
     6121 ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat
     6181 ccggaccaat accatctgca tattccgcca cctgtttcat ggctcccggc ttaaacatcc
     6241 agtcgtagtt gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt
     6301 cggtataggc aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat
     6361 tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac
     6421 cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct
     6481 gatgatggaa ccacggcgct ttgatttctg gatagatacc gatatttttc ccggtagagt
     6541 gatttaaccc ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact
     6601 tacccattgg gaaacgcccc ggataagtct gtactttttt accgttttca atatcgaaac
     6661 cttcggtaaa tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac
     6721 cgtctttgcg cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat
     6781 ggtcatgcag aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat
     6841 ccgctccctg cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac
     6901 tggcaccgcg atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc
     6961 ccatgactat agtgctcatc atgatcgcca tgctaaggtt tttcagcttc aatttcatgc
     7021 cattagcctc cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc gccaatcatc
     7081 acaacaatca acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca
     7141 tcccagccga agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc
     7201 aggtaaccaa acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt
     7261 tccagcgcat gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc
     7321 atacaaatca tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg
     7381 atggtcacca gtgtcataaa gaagacgccg gttgccccac ggttaccacg gaagactttg
     7441 tccgacatcc agccgcacag cagggtgccc ggaatacccg catactcata gaagaagtag
     7501 gcccaggagg atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag
     7561 tcgaggatgc cgtaacgcag cagataaacg aacacgttgg cgatggcgat gtaccacagc
     7621 agtttgttcg gcagcacgta ctgcatgaag atttgcttcg cggtcagctc ctgttccgct
     7681 ttttcgttat agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag
     7741 gattgcgggg tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa
     7801 gcaggcatat agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc
     7861 agcggcggaa taccaccacc gacgttgtgc gcacagttcc acactgacac aatgccgcca
     7921 cgttctttct gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc
     7981 tggaaccaac cgcagaggaa caacagtaca aacatcaccg caatgctcga cgtcgcccat
     8041 ggcacaaagc ccataaacaa catcactgcc gccgccagaa tcaaacctgc gggcaggaaa
     8101 acgcgcggat tcgagcgatc cgataccgaa cccatgatga atttcgaaaa tccataagca
     8161 atcgagatcc ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg ctcaaccaga
     8221 taaggcatag caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat
     8281 atccccagga aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcggcaggt
     8341 aagcgcgctt tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg
     8401 tggtcttatt tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc
     8461 cgcgatgtta agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa
     8521 gatttacaaa atgttcaaaa tgacgcatga aatcacgttt tactttcgaa tcatgagcga
     8581 atatgcgcga aatcaaacaa ttcatgtttt tactatggct aaatggtaaa aaacgaactt
     8641 cagagggata acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg
     8701 cggcgcaacg ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt
     8761 ggttgagcgc cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag
     8821 cggtgcgcgc tatgcagtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca
     8881 gatcctgaaa cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct
     8941 gccggaagat gacctctcat tccaggccac ttttattcgc gcctgcgaag aagcagggat
     9001 cagcgcagaa gctatagacc cgcagcaagc gcgcattatc gaacctgccg ttaacccggc
     9061 actgattggc gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc
     9121 aaacatgctg gatgccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg
     9181 gctgattcgt gaaggcgcga cggtgtgcgg tgttcgtgta cgtaaccatc tcaccggcga
     9241 aactcaggcc cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat
     9301 tgccgaatat gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat
     9361 ggatcaccgc attaaccagc atgtgatcaa ccgctgccgt aaaccttccg acgccgatat
     9421 tctggtgcct ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa
     9481 cgagattgac gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga
     9541 aaaactggcc cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc
     9601 gctggttgcc agcgatgacg acccgagcgg acgtaacgtc agccgtggca tcgtgctgct
     9661 cgaccatgct gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat
     9721 gacctatcgg ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac
     9781 gcgcccctgt acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac
     9841 cttgcgtaaa gtcatctccc tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg
     9901 cgatcgcacg cctgcctggc tgagcgaagg ccgtctacac cgtagcctgg tatgtgagtg
     9961 cgaagcggta actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct
    10021 gctggattta cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc
    10081 ctgccgcgct gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga
    10141 gcaactttcc accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga
    10201 cgcactgcgc gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa
    10261 ggagcagaaa gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct
    10321 gtggcctgca actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg
    10381 cgttgcattt ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg
    10441 tggcagacat tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact
    10501 ctcttctcgg gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg
    10561 aaagcggtgc gcaattgcag ggcagcgtag aactggctca ccagcggatt acgccgctcg
    10621 gcactttgcg tgcaacctgg ttaagttcgc cagaagttcc cgtctggccg ctacccgcga
    10681 agaagatatg tgtagtgggg ataagcgggc tgatagattt ccaggcgcat cttgcggcgg
    10741 cttcgttgcg cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg
    10801 atgtcctgcg caataacgcc accgaatttc gcgcggtgaa tatcgcccgt ttccttgata
    10861 atgaagaaaa ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa
    10921 tgatcctgat gcccgcctgc ttcggcctgg ccgatgacaa actgtggcgt tggttgaatg
    10981 aaaaactacc ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc
    11041 gtctgcaaaa ccagttacag cgccagtttg tgcgccaagg tggagtgtgg atgccgggcg
    11101 atgaagtgaa aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc
    11161 acgccgatat tccgctacgt ccacgtttcg cggttctcgc cagcggcagt ttctttagtg
    11221 gcggactggt agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac
    11281 aaaccgccac gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc
    11341 agttcggtgt aaccactgat gagacgctac gcccgtcgca ggcagggcaa accattgaga
    11401 acctgtttgc catcggttcg gtgctgggcg gatttgatcc catcgcccag ggatgcggcg
    11461 gcggtgtttg tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag
    11521 gccaacaatg aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc
    11581 ctgcccggtg agccgggtga atcccggtta tccagggcca aaacaagccg ggccggatgg
    11641 cgagcgtctg cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa
    11701 ctgcaaacgt tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg
    11761 cgcgcgggcg aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac
    11821 cgacctgatg ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct
    11881 gaaaccggtg cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc
    11941 gaaatactcc ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca
    12001 atataaagac caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt
    12061 aggtaaagat ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa
    12121 agaaaaatgc tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca
    12181 ggcaattacg aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat
    12241 tgccacctcc tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt
    12301 cgacaacaaa ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct
    12361 ggacgaaggc aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc
    12421 gtgccatatg gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaacatccc
    12481 ggggcttgag ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt
    12541 caaaaaagag aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga
    12601 agaaagcggc gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat
    12661 gtccacaagt cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac
    12721 tcccttctga tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg
    12781 cccaatgtat gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa
    12841 actggcagat gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa atatcaggtg
    12901 acggacaacc atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt
    12961 tatattcaca cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg
    13021 caagctttgc aacctgcaaa ccttacgcct ggaacccact agttttattg aaaaaagttt
    13081 acgcgcttac tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat
    13141 ctactgcgtg attgaacatc aaagctctgc agaaaagaat atggcttttc ggccaatgcg
    13201 ctatgccact gccgccatgc agagccacct agataaaggc tatgacagag ttccgctggt
    13261 ggtgccattg ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct
    13321 ggatgagttt gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt
    13381 ggatatcacc atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga
    13441 actgattcaa aagcatattc gcgaccacga tttaatcggc atggtcgaca ggatcaccac
    13501 gcttttggtt agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct
    13561 gcaatgcggc gatacctccc gtttcacccg ttttattcag gagattgccg aacgttcacc
    13621 actacaaaag gagagattaa tgactattgc tgaacggcta cggcaggaag gacatcaaat
    13681 tggctggcaa gaaggtaaat tagtaggttt gcagcaaggt aaattagaag gtttgcagga
    13741 aggtatgcat gaacaagcca ttaaaattgc tttgcgcatg ctggaacagg gcattgatcg
    13801 tgaccaagtg ctcgcggcca cccagctaag cgaagccgat ctggcagcga ataaccacta
    13861 attaacacag gcccacagcc gatccccatg ggcctttgat atcaataact accttttatg
    13921 cgtggcccat ttttgcctga tttaaacatc gccagtcgtt gatccagggc atcgctgtag
    13981 agcatcgtgt caacgccaac agcgacaaag ttcgctcccc acgccaggca ttgctgcgcc
    14041 atatcaggag ccacagccag aaaaccagcc gctttacccg cagcacggat ccgccgaatg
    14101 ctggtttcaa taattcgctg cacttccggg tgcccggcgt tatccgggta gcccaacgac
    14161 gcagaaagat ccgctggtcc aataaacacg ccatcaatcc cttcgacgtc gaggatctcg
    14221 tccaggttat ccagtgccgt tttactttcc acctgcacta acagacaaag cgaatcgttg
    14281 acttgcgcca tgtaattctc aatgcgtccc cagcgcgcag cccgtgccac actggcccca
    14341 acaccacgct caccgtaggg agggttggac aaattttgcc ggagggggaa tgtgccggtg
    14401 cagtaaaccg gcacagagtg gcgaaatcag acggataaag tcatggactc aacaacctca
    14461 atccagccat gctcactagc gatgtcctgg ccgtttaacc aacggcgtag catattcagt
    14521 gccatcattg cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc
    14581 acacgcagag cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg
    14641 aaacccgaaa cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag
    14701 tgcgcagttt gcgccagggt ttcttcctgt gaaggaatca cttcacacgc cagcaatgga
    14761 gcacctgcgc gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc
    14821 aggctgaact ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct
    14881 tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc
    14941 tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag
    15001 cccattgtta cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc
    15061 gaacgaccaa aggtagttaa acgcagacaa accggcggct gtggtaaaga aaaacgctcg
    15121 cgcaggcgcg gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga
    15181 gtaaagaaca tcaggcaacg attaagctgc acggcaaaac cacaggcggt gcctaccggg
    15241 ttattgataa attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt
    15301 actcgtccac gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc
    15361 aggccttcac cttttgctgt cgcagcggcg agtgcgctta aatcatcgct ggtcggtccc
    15421 agcccgccgt taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact
    15481 aagtcatcaa ggttatcccc caccgtattg cggcgagata atggcagccc ctgatgaaag
    15541 aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt gcccgtataa cacttcatcc
    15601 ccggtggata gcatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact
    15661 ataacgcaaa gggagataag gcgtagtaag aaactgaatg ccgcgccgga agggatcaga
    15721 aactggcgtt aaccccaaca tacaggccgt cagccacagc gttgtcgcgg ttaccgtctt
    15781 tacccgacag attcaggtag cgataacccg cctcaatact gactggacgc ataatggtgt
    15841 aacgcgcgcc agcattcgct tcctcataac tttgaatacc gctggagagc gaatccggag
    15901 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    15961 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtacacg ccttttccgc
    16021 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg cccgcgacgt
    16081 caccgtcgtc gtcgttatgt gtccagttac cgcttaaagc caggcccgtc gattcagtcc
    16141 caaaaccgac accaatattg gtgtattctt tacccgcctg gccgctgatg ctcattgcat
    16201 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    16261 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    16321 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    16381 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    16441 gtgcagttat cattgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    16501 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    16561 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    16621 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    16681 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    16741 ccgtggacct tcagcgatga tattcgcacc aagacatatg cagatggtcg caaggaagag
    16801 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    16861 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    16921 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    16981 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    17041 ttttccacat gcatgactaa accatccaga taatcaggtt taaatatcgc atcacgctta
    17101 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    17161 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    17221 ttatctaccg tcagcttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    17281 gtattactgg aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca
    17341 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    17401 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    17461 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    17521 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    17581 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    17641 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    17701 tacgatgtcg gcaaaatgcc tcaaattttt gccaaatgca aagcctaaat aagaaaaaat
    17761 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    17821 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    17881 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    17941 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    18001 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    18061 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    18121 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    18181 tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    18241 cctgaagcga tcgaatcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    18301 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    18361 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    18421 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc tgaaggtggc
    18481 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    18541 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    18601 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    18661 gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca
    18721 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    18781 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    18841 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    18901 aaatattatc gcgagcgttt tcccacactg tcgttaccga ataccgaatg gaatagcgaa
    18961 cgcatctgtt cgttgccgct gttcccggat atgactaccg ccgatgccga ccgcgtcatc
    19021 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    19081 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    19141 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    19201 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    19261 gtgtctattt tgattaaccg caattacggg caacattcag cgattatggc gggattcagt
    19321 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    19381 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    19441 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag
    19501 cgcaccactg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    19561 attgtcgatg cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat
    19621 atcttcgccc gccgtgcaat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    19681 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    19741 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    19801 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    19861 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    19921 ttactcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    19981 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    20041 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    20101 gcgccatttt tacccatacc gataatcccg gtgaaaaagc cttttatggt tcggtggctc
    20161 atctggcggc ggaaagagac attccggttt atgcgccgga taacgttaat catccgctgt
    20221 gggtggaacg cattgcccaa ctgtcaccag aggtgatttt ctctttttat tatcgccatc
    20281 ttatttgcga cgagattttt cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    20341 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    20401 aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc
    20461 aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc
    20521 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    20581 aaatcgccca gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt
    20641 tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc
    20701 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    20761 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga
    20821 ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta
    20881 tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc
    20941 agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    21001 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    21061 atattggcag cgatgcgata agccgttttc tgaatcatcc acattttcac tttgtcgaag
    21121 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    21181 tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    21241 aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa
    21301 tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    21361 accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa
    21421 aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc
    21481 tcttcctccc gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg
    21541 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc
    21601 taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt
    21661 tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca
    21721 atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa
    21781 aacatccgct gcgccatcat ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagct
    21841 gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    21901 accgctgcct ggactgggag ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    21961 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag taggcttacg
    22021 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt
    22081 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    22141 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    22201 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    22261 tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gtctgcacgc
    22321 ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga
    22381 cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc
    22441 tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt
    22501 gcgctacaac agcgattgtc gtggggccat accgttccgt ccactgcttg aatcaggaaa
    22561 ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    22621 tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac
    22681 gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga
    22741 tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc
    22801 agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    22861 gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcatc
    22921 ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc
    22981 gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg
    23041 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    23101 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    23161 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    23221 ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    23281 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    23341 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    23401 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    23461 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    23521 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    23581 cctgacttct ggcactattt tttctgggtt gagcatattc aacgctttgc actagatgat
    23641 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    23701 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    23761 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc
    23821 aaattgccca cctatattct ttcttgcttt gcacctctgg caatgctgtt ggcgcattac
    23881 gcgttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    23941 gcttttggcg tcactggcat tattgccaca tttgtgatct ccccctgggg accaatgaac
    24001 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    24061 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtctttcgcc
    24121 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    24181 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    24241 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    24301 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    24361 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    24421 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    24481 ccgcccgcag atgccatcgt tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    24541 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    24601 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    24661 gactggcgct ggcttgtctt ggtcttgcca tgatgctctg gctgctggtc ttgcagaacg
    24721 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    24781 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    24841 ttggtggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatatggg gattattcag
    24901 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcaagtc atctgccgcc
    24961 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    25021 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    25081 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    25141 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    25201 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    25261 agttttccct gccgctgtac actgccgttc ccactcatcg gggctataac tgcttgccga
    25321 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    25381 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    25441 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    25501 ttttttgtgc cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
    25561 acagattaac agataaactc tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa
    25621 cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta
    25681 ataccgccgt ttttcagctc cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga
    25741 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
    25801 gctaccggcc gatgaccaaa ctccttgtcg gcaacggaaa cgataaacac ctgcaaaacc
    25861 gcaggatgtg cagcaataac acgctcgact tcttccggct gaataccctc accgccactg
    25921 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
    25981 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
    26041 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
    26101 cgacccggca gcggcaaacc aacgtctgcc aggccgtcgg cttctttcgc acataccgtg
    26161 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
    26221 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
    26281 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
    26341 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
    26401 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    26461 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    26521 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    26581 agacgcgtcg gctgccacgc agcggcatgt acgccttcaa ccagctgaat gtgcagcgac
    26641 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    26701 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    26761 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    26821 atcacgccgc tgccctcaac caccccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    26881 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    26941 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcct
    27001 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    27061 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    27121 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    27181 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    27241 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    27301 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    27361 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    27421 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    27481 gatccggaat tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca
    27541 atcccacttt gaccttcgcc actttctcgc ccggcatatc ggcaagtttg aggatcagat
    27601 cgtccggatc gccattacac agcggtgccg cacggtagtt ggctgcttgc ggcaacgtat
    27661 ctgtcagttc tgccaatgca cagcttacgc caaaggccac ggaaggcatc tgcggtagct
    27721 cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc
    27781 agttttcctg gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct
    27841 cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc
    27901 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    27961 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    28021 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc
    28081 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    28141 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    28201 gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc
    28261 tcgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa
    28321 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc
    28381 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    28441 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    28501 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    28561 cgctttatcg cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc
    28621 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    28681 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcgaa
    28741 accttcggag cagtcgtgcc attcaaccgg tgcgtaaggc attgcttcat caggataaat
    28801 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg
    28861 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    28921 tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    28981 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    29041 atggcggcaa gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga
    29101 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    29161 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt
    29221 tcagcatttt gcagccccgg atgcccgcct tcgacaataa ccccacaaag ccccgccagc
    29281 ccctggcaag ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag
    29341 tcaaggatgt tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca
    29401 tcgacgctaa tcgccgccga accaccatga cctaggagat caacatacaa ccgtgagtag
    29461 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    29521 aacaccagcc agggtaaacc tagttttccg tgttttgcct gcgcgtgcag gatcataaat
    29581 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    29641 tttcaatcac cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct
    29701 gccagttttg cggatgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    29761 cgttttgcgg catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga
    29821 aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca
    29881 ataacgccag cgcgttgaga tcgtaaagcg cggagagatc gcccacaatc gccagcgtcg
    29941 gtttgccgct tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg
    30001 cccctcggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    30061 gtaccaccag gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    30121 gatgcgccag ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct
    30181 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    30241 ccagccagtc ggcaatattg gcaattaagc gacgtccgcg atggtgtgcc ggatcaagtc
    30301 gcccttcaat gtcatcaaca atccagtact cttctggttc acagcttgcc tgccattgca
    30361 ggagccgttt ccccgtcagg ctacttccca gttgcaccac aatttgcgcc tgctgcagct
    30421 cgctggtcgc tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt
    30481 gtgacagcac atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt
    30541 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga
    30601 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    30661 cctgccacca gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat
    30721 acagcggttc agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag
    30781 cgtggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    30841 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc
    30901 cgcagtcaat tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta
    30961 acccggcttc aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca
    31021 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    31081 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    31141 tagaacgcga gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    31201 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    31261 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    31321 gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    31381 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    31441 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat
    31501 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    31561 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    31621 aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    31681 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    31741 aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc
    31801 atcagccact ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc
    31861 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    31921 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    31981 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    32041 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    32101 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    32161 aacccaggca agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca
    32221 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    32281 cgtcgccatt ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc
    32341 ccccaaatgc gtaagtcagc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    32401 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    32461 tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    32521 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    32581 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    32641 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    32701 aggttttaac ggcgtgccag caacagtcct agtaccagcc cgacggccgc gcccacacca
    32761 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc
    32821 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    32881 tctgcatgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    32941 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    33001 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagagtt
    33061 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    33121 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    33181 gccccgaggt aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc
    33241 atcagctgct gccccacttt ttcaccacgc aacgcttcac tgacaatcac ccgacctata
    33301 acgaccggct caagatcatc atcacttttc agaatcctcg catacgccac cagttcatca
    33361 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    33421 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    33481 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    33541 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    33601 atttttttag gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    33661 cttaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    33721 cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt
    33781 caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca
    33841 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    33901 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    33961 ggcgaaattc tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg
    34021 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    34081 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    34141 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    34201 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    34261 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    34321 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc
    34381 ggcaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg
    34441 ttacgtgaat gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt
    34501 aacgtttaac tctgcttatt attaaaaaca gggtgaaact tgccctgtta tagcaacccg
    34561 cgctacatat atccacccac acaggcatat tgagcgattt ttgatattta ttcatctcct
    34621 tacgcactat attcagagca ataagttgcc tcttttcatc ttgattcagc acaacctttg
    34681 caactaaaag gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac
    34741 ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt
    34801 aaaaaaaaca gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt
    34861 aggttatgat gaatttggaa aaaaagctgg gtaatgctgc caacttactg atttagtg
//
LOCUS       DAECOD010000033        34587 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.033, whole genome shotgun sequence.
ACCESSION   DAECOD010000033 DAECOD010000000
VERSION     DAECOD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.033"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1101)
                     /locus_tag="KKO15_001654"
     CDS             complement(<1..1101)
                     /locus_tag="KKO15_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBD6360472.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLYAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="KKO15_001655"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="KKO15_001655"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBD6360473.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="KKO15_001656"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="KKO15_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBD6360474.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="KKO15_001657"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="KKO15_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBD6360475.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="KKO15_001658"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="KKO15_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBD6360476.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="KKO15_001659"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="KKO15_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBD6360477.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="KKO15_001660"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="KKO15_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBD6360478.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="KKO15_001661"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="KKO15_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBD6360479.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="KKO15_001662"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="KKO15_001662"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBD6360480.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="KKO15_001663"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="KKO15_001663"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBD6360481.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="KKO15_001664"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="KKO15_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBD6360482.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="KKO15_001665"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="KKO15_001665"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBD6360483.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="KKO15_001666"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="KKO15_001666"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBD6360484.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="KKO15_001667"
     CDS             11851..12015
                     /locus_tag="KKO15_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360485.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="KKO15_001668"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="KKO15_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBD6360486.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="KKO15_001669"
     CDS             13524..13769
                     /locus_tag="KKO15_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBD6360487.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="KKO15_001670"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="KKO15_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBD6360488.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="KKO15_001671"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="KKO15_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBD6360489.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="KKO15_001672"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="KKO15_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBD6360490.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="KKO15_001673"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="KKO15_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBD6360491.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="KKO15_001674"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="KKO15_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBD6360492.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="KKO15_001675"
     CDS             19432..19785
                     /locus_tag="KKO15_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBD6360493.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="KKO15_001676"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="KKO15_001676"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBD6360494.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="KKO15_001677"
     CDS             21084..22211
                     /locus_tag="KKO15_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBD6360495.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="KKO15_001678"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="KKO15_001678"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBD6360496.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24393)
                     /locus_tag="KKO15_001679"
                     /pseudo
     CDS             complement(24291..24393)
                     /locus_tag="KKO15_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24447..26421
                     /gene="pdeR"
                     /locus_tag="KKO15_001680"
                     /pseudo
     CDS             24447..26421
                     /gene="pdeR"
                     /locus_tag="KKO15_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26569..26742
                     /locus_tag="KKO15_001681"
     CDS             26569..26742
                     /locus_tag="KKO15_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360497.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26832..27581
                     /locus_tag="KKO15_001682"
     CDS             26832..27581
                     /locus_tag="KKO15_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6360498.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27850..28068
                     /gene="osmB"
                     /locus_tag="KKO15_001683"
     CDS             27850..28068
                     /gene="osmB"
                     /locus_tag="KKO15_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBD6360499.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28194..28520)
                     /gene="yciH"
                     /locus_tag="KKO15_001684"
     CDS             complement(28194..28520)
                     /gene="yciH"
                     /locus_tag="KKO15_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBD6360500.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28520..29257)
                     /gene="pyrF"
                     /locus_tag="KKO15_001685"
     CDS             complement(28520..29257)
                     /gene="pyrF"
                     /locus_tag="KKO15_001685"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBD6360501.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29450..30619)
                     /gene="lapB"
                     /locus_tag="KKO15_001686"
     CDS             complement(29450..30619)
                     /gene="lapB"
                     /locus_tag="KKO15_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBD6360502.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30626..30934)
                     /gene="lapA"
                     /locus_tag="KKO15_001687"
     CDS             complement(30626..30934)
                     /gene="lapA"
                     /locus_tag="KKO15_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBD6360503.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31083..31847)
                     /gene="pgpB"
                     /locus_tag="KKO15_001688"
     CDS             complement(31083..31847)
                     /gene="pgpB"
                     /locus_tag="KKO15_001688"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBD6360504.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32017..32607
                     /gene="ribA"
                     /locus_tag="KKO15_001689"
     CDS             32017..32607
                     /gene="ribA"
                     /locus_tag="KKO15_001689"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBD6360505.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32671..>34587)
                     /gene="acnA"
                     /locus_tag="KKO15_001690"
     CDS             complement(32671..>34587)
                     /gene="acnA"
                     /locus_tag="KKO15_001690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBD6360506.1"
                     /translation="LTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPL
                     ADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGD
                     EPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRLPVDY
                     VMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAP
                     GSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKGDLTVGAV
                     LSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGEPVYLKDI
                     WPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQEDSTYIRLSPF
                     FDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDF
                     NSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQ
                     TPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQG
                     VTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQ
                     NDGILHYVIRNMLK"
BASE COUNT         8148 a         9016 c         8736 g         8687 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tataacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaatgat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgtcttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaagaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg ttcccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc acacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgttg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcgatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa aagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc ctgccgcagc ctgctccggt cggacctggc aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccact
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaacaat
    24361 gcgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact
    24421 ccgctgttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca
    24481 actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat gctttgcgct
    24541 tttctaccac caaaaccaca gaacctgatc gtacattgca gttatctgcc gaacaggctg
    24601 ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg
    24661 aaagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca
    24721 acgcgtctgc atggcatgac acaccggcgg ttgctcgtga tttatcacac gggctttcct
    24781 ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga
    24841 atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaaagaa cacgacgtca
    24901 ttgggtaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca
    24961 ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac
    25021 gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa
    25081 acgagatttt tttaatctgt tccggtaccg acattaccga agagcgccgc actcaggagc
    25141 gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc
    25201 aggagttaat cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc
    25261 ttgatttgga taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt
    25321 tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc
    25381 gtccaggtgg cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag
    25441 caatggcatc acgaattttg acccgcttac gcctcccctt tcgtattggt ttaattgaag
    25501 tttataccag ctgttcagta ggtatttcgc tctctcccga acatggttcg gacagcgcga
    25561 ctattattcg tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat
    25621 tttgcgtttt tactccagaa atgaatcagc gggtatttga atatctctgg ctggatacca
    25681 acttgcgtaa agcactggaa aacgatcagt tggttatcga aaatcaccta gcgtggcgaa
    25741 gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt gattccaccg
    25801 ttggacttca tttcctacgc cgaagagtca gggctaattg tgcctttagg ccgttgggtg
    25861 attctcgatg tcgtacgcca ggtggcaaag tggcgggata aaggcataaa cctgcgagtg
    25921 gcggtaaata tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag
    25981 gttctccagg aactcaattt tgaatactgc cctatagatg ttgaactgac agagagttgt
    26041 ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagcca actaggtgcg
    26101 caagtgcatc tggatgattt tggtaccggc tactcttcac tttcgcaact ggcgcgcttt
    26161 ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag atattcacaa acaacctgtc
    26221 tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg cattgaatct tcaggtgatc
    26281 gccgaaggtg tagagagtgc aaaggaagat gcttttttaa ccaagaacgg gatcaatgag
    26341 cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa
    26401 cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata tcccgtcatt
    26461 atatatcgta ttcctggatg ataattagcc gttgctgtta aacacttctc ttttagcggc
    26521 aatttttgca ttattcattt tccccctttt gctataagga tgtccttgat gtctgaattt
    26581 gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat
    26641 taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct
    26701 gaatctacgc cagacatccc gaagacaccg tgggagattt aacaatcgtg ccttcaggtt
    26761 ttcctatttg aggatgcccg gcgcgtcagt atttttttat ttagtattat aacgttataa
    26821 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
    26881 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
    26941 atctcaacac cctcgaaaaa ctgagttacc tccgccgtgc acatggcttt gcagtttcgc
    27001 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
    27061 ccgagtttgc cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggcagca
    27121 gcaatgccct gctcgcccgg actctggggg agcagaagaa aaatgtcact atcatcacgg
    27181 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg
    27241 gcgtgtacca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccagc
    27301 aggtacattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actggattca
    27361 ccggtcgcga tatgatgcgc accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag
    27421 cgatagtcct gactgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg
    27481 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
    27541 ttgagcacag taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg
    27601 atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact
    27661 atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat aacaggaagt
    27721 aattatcacc tgcgtgatat aaccctgcgc gcgagcagat ttcacggaat aatttcacca
    27781 ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga
    27841 gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattactttg
    27901 gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggc
    27961 gcgggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta
    28021 ggtggtgcag ctgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat
    28081 gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat
    28141 aatacacaca gtaaaatatt cgaaagccac ggatatatcc gtggcttttt atgttaaccg
    28201 cctgcgagtt ttaccttcat ccctttcgct tcaagtagtg attttaataa atcacgctta
    28261 tcgccctgga tttcaataac tccatcttta actgctccgc cgcagccgca ttttttcttc
    28321 agttccgctg caagttttgt cagttcggca tcatcgagat cgataccggt aatcaggcaa
    28381 acgcccttac ctttacgtcc actggtctga cgctgaatac gcaccgctcc atcgccttta
    28441 gggcgtactg gggccgcttt gggctcatca atacgcccgg tttccgttga gtagaccaga
    28501 cggctgttgg aatcactcat catgcactcc gctgtaaaga ggcgttgatc gctttcagtg
    28561 tctgcgctgg atctaccgat tgcgttaccg ggcgaccaat caccatataa tcaacaccag
    28621 ccgccaacgc ctgttctggc gtcataatgc ggcgctggtc accagcttca ctcccctgcg
    28681 gacgaatgcc cggtgtaacc agtttgaact cctgaccgaa tacctgttta aagcgcacag
    28741 cttcctgagc agaacacacc acaccatcaa ggtcacattt ttgcgtcagt gctgccagac
    28801 gttctgcata atctgcaggt gacagtgtca cgccaagatc ggccaggtcg ctggcttcca
    28861 tgctggtcaa cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagtg
    28921 cctcacgcgc tgcggtcatc atacgcgccc caccggaggc atgaacattc accatccaca
    28981 cgcctaagtc agctgcagca gcgacagcgt gcgctgcagt gttggggata tcgtggaatt
    29041 tcaggtcaag aaagatatca aaaccacgct gttgaagttc gcgcacaaac tgtggcccaa
    29101 acaatgtaaa catctcttta ccgaccttca gacgacaatc gcgtgggtcg atcttgtcga
    29161 caaaggacag cgcgtcatca caattatgat aatcaagggc aacaaccaca ggagaattcg
    29221 taacggcgcg ggaagaagat gaagcagtta acgtcatgac cagaccttct tgatgatggg
    29281 caccgggagt ggcgcgaaac aggtaaacgg cgagcattct acccgtgaaa ataaaaaatt
    29341 aacagttgcg atttttcccc tagcaggctt tttctgctcc gtcgttgccg gagcacaatg
    29401 ggacatcaat aactagtatg ttgtaactaa agtcggattt ttttaaaaat tacaggccat
    29461 caagaccgcg aatcggttta atagttgacc aggcacgaca agacggacaa tgccagtaga
    29521 gggtgtatgc ggtaaaacca catttctggc agctataacg aggcttacta cgcaccttct
    29581 cgccaaccat gtcacgcagc accatcagac tctctttggc gcgcccttct tccgcttcat
    29641 ttaagtggta atccattagc ttatggaata cacgcatggt cggatgacgc tgaagctggc
    29701 gtgtaatata gacctgtgca gcctcactac cgtcgcgcgc ttcgatgata tcagcaagca
    29761 tcaattcagc atcggcaccg gtgttctctt ccaccgcgcg ttgcaggaat tctgcccatt
    29821 cggcagtttt acccaactgc tgatagcagg tttgcaacat ttccagcgtt tcgctgacca
    29881 gttctctgtc ctgggatatg acgcgttgca aactttcgac ggctttggcg tattctcctt
    29941 tcgccataaa cacgcgtccc atcatgatgg atacgcgggc gctgttttta tctgccgccg
    30001 cccctttttt tagcaaggtc atggcacgat cgagatcgtc gctggccata tgctgcaggg
    30061 ctaactcaca gtagaaatgg gcaatttcga cgcgctgttt atctttaccc agcttcacca
    30121 ggcgttcggc aacatcaatt gctttctgcc actcactggt agcctggtag atttgtagca
    30181 actgttgcag cgcgccaatg cggaagtcag tttcatcggt cagctgattg aacatgtctt
    30241 ccgcgcggtc gtataacccg gcggccatgt aatcacgccc cagttgttga atcgccaaca
    30301 gacgctgttc ataggtcagc gaggcgcttt ccattagggt ctgatggatg cgaatagcgc
    30361 gatcaacttc gccacgcgaa cggaacaggt ttccgagcgt aaggtgggct tcaacggtgc
    30421 ctgtatcctc tttaagcata tcgagaaaca ggtctaccgc tttatcctgt tgattactaa
    30481 gcaggaagtt aacccccgct acgtaatcac gcgacaagcg gttggcttca tcttgcttgt
    30541 tttgttgcgc acttctgcgg cccatatacc agccataggc agcggctaca ggcaaaagca
    30601 gaaacaacaa ctccagcata gaaagttatt ccttcgccgc tgacgagtgc ggcactacag
    30661 ccacgtcagt cgcgggtgaa agctggtttt ccagtcgctt tattttacgt tcagcgcgcg
    30721 ccagggaaac acgaactcgc agccagaaca ggccacaaat caaccaaccg atagcaaacc
    30781 ccgcagcaaa caataccgcc agcaatgtgg aaatacggta ctccccttgc gctaacagat
    30841 aattaaacgt cacctgttga tcgttctgcg cacccaacgt gaccgaaatc acgaagatcg
    30901 ctaacaccag taagaaaatg agtaaatatt tcacattact tcccgttatg tggttagagc
    30961 gaataaatta tggtcaactt accgcaatag ccctttaaag taccattttc gcgccgtctg
    31021 cgaaatggca tccacgcgca ttgttgatga tttaaggcct aaattcggaa aatcaaccag
    31081 cgttaacttt cttgttctcg ttgtgcaatt tcgcgatttt cttccgcagg tggtgttaat
    31141 ggcccacaaa ttcgttgcgc aagccacgtt gccaccgcca ccagcgccca cgaaatcaac
    31201 gtagctacca ccagatcgcg tggccaatgc atcccgagca gcaggcggct tcccatgact
    31261 cccgttgccc agaccaacaa gatagcaatg gttaacgttc gccgacgcgg ccacagcaaa
    31321 ccaacggcca gcagtgccca actggcagca aacatcgtgt gaccggaagg aaaggcaaac
    31381 cccgtctctt tctgccagtg tgaacgcaaa tattgtggaa tatttttctc ttcagccaac
    31441 tgttctttta ctagatttcc gcgttctgct cgctttaaag tgtagaactc atcaaccgga
    31501 atatgatgtg ttttttccaa ccagataaca aaaggtcgtg gttcctgtac tttgtctttg
    31561 atccaggatt taacgccttg tccaacaagg attgccgccg ccagaatggc aaataataca
    31621 aaggcagcct taatgcgaaa acgcagacac cagagaaacc agccgaacaa aatcaaatgt
    31681 gtaatgacgc cccagggctg ggtgacagtt tcagtaaccc aaaaagccgc ttttagtagc
    31741 caactttgtt ctccaggttg ccaacgccag ccagaaatcc atacggctac tggcatgaca
    31801 agcaatagtg cagctcccac tgcggtacgt cgggcaatcg aacgcatggc ctctcctttt
    31861 tgataagtcc cacaatcata actgaaaatg ccagttccaa gaaaaattga cagatttgtg
    31921 ccattccgtg aacgatcgac gcgtcgtgat taggtgaacc ccttctcgtt atggcaaaat
    31981 aagccaatac agaaccagca ttatctggag aatttcatgc agcttaaacg tgtggcagaa
    32041 gccaaactgc caaccccatg gggcgatttc ctgatggtgg gatttgaaga actggcaacc
    32101 ggacacgatc atgtcgcgct agtctatggc gatatttccg ggcatacccc ggtacttgcg
    32161 cgcgtccatt ccgaatgtct gaccggtgac gccctgttca gcttgcgctg cgattgtggc
    32221 ttccagctcg aagcggcatt gacgcaaatt gccgaggaag gccgtggtat tttgctgtat
    32281 caccgtcagg aaggtcgtaa cattggtctg ctgaataaaa tccgcgctta cgcactgcag
    32341 gatcaaggtt acgataccgt agaggctaac caccagttag gcttcgccgc tgatgagcgc
    32401 gacttcactc tttgcgctga tatgttcaaa ctccttggcg tcaatgaagt ccgcttgtta
    32461 accaataacc cgaaaaaagt cgaaattctg accgaagcag ggattaatat tgttgaacgc
    32521 gtaccattga ttgtaggtcg taaccccaat aacgaacatt atctcgatac caaagccgag
    32581 aaaatgggcc atttgctgaa caaataaccc tcttgcatcg tgtaattcat ttgcttgccg
    32641 gaagcaaaat aaccggcaag caaatacttg ttacttcaac atattacgaa tgacataatg
    32701 caaaatgccg tcgttctggt agtaggtcaa ctccgtcgcg gtgtcgatac gacaacggca
    32761 gggtacgact tcctggctac catccgcgcg cgtaagcgtc accggaaccg tcgcgccagg
    32821 ttgtagattt tgcagatcgc caatatcaat cttctcttcc ccggttagcc ctaacgtttt
    32881 acgcgtcacg ccttgcggaa attccagcgg caggatgccc atgccaatta aattcgaacg
    32941 gtgaattcgt tcaaacgatt cggcaatcac cacacgaatt ccaagcagac gaggtccttt
    33001 tgccgcccag tcacggcttg agcctgatcc atactctttc ccggcaatca ccgccagcgg
    33061 cgtttgctcc tgcttatagc gcatcgcagc atcataaata gagactacgt cgctgtcagg
    33121 taaatgccgc gtcatccccc cttcaacgcc aggcaccatt tcattacgga tgcgaatatt
    33181 ggcgaaggtg ccgcgcatca tcacttcatg gttaccacgc cgcgaaccgt aggagttaaa
    33241 gtcttttcgc tcaacacccc gaccttgtag atatcgaccc gctgggctgt cgggcttaat
    33301 actgcccgcc ggagagatat ggtcagtggt gactgaatcc cccagcattg cgaggatccg
    33361 cgcaccgtga atatcttcca ctggtgctgg tgttgcctgc atttcatcaa agaaaggcga
    33421 taagcgaata taggttgagt cctcctgcca accgtaggta tcggatcgtg tgacgttaat
    33481 ttccttccac tctgctgtgc cttcaaaaac ttctgcgtac tctttgcgga acatttctgt
    33541 ggagacttgt tctaccgcac gggcaatttc ttgtgccgat ggccagatat ctttcagata
    33601 aaccggctcg cctttgcgat catggccgat aggctcagaa gccaggttga tattcatatt
    33661 tcccgccagc gcataggcaa ccaccagcgg tggcgaggcc agccagttag ttttaaccag
    33721 cggatggata cggccttcaa agttacggtt gccggacagc accgcaccga cggttaaatc
    33781 gccttttttg attgccgttt cgataggatc gggcagcggc ccagagttac caatacaggt
    33841 ggtacaaccg tatcccacaa ggttaaaccc cagttcgtcg agatacggtg tcagtttcgc
    33901 ttttgccaga taatcagaaa cgactttcga acccggtgcc aacgacgctt tgacccacgg
    33961 ttgccgcttg aggccaagag ttacggcttt tttcgccagc aagcctgcgg ccatcagcac
    34021 acttgggtta gaggtgttgg tgcacgaggt tatcgcagca atgaccacag cgccatcagg
    34081 taactgatac tgatgtccgt tcataacata atcgactggc aggcgatctt tatgcgtggc
    34141 attcacttcc agttcgttac tggcggcaaa tgcttttggt acatcgggca gtgcaacgcg
    34201 atcctgtggg cgttttggcc ctgccaggct cgcttcaacg tcattcatat ccagttctaa
    34261 catactggta aaaatcggtt catcacccgg gttacgccac atgccctgcg ctttggcata
    34321 tttttcgacc aactcgacct gatcttcgct gcgcccgctt aaacgcatgt aatcgagggt
    34381 tactgcatcg attgggaaga agccacaggt ggcaccatat tctggcgaca tattggcaat
    34441 ggtggcgcga tccgccaacg gtagtgaatc cagaccatca ccataaaatt cgacgaattt
    34501 ccccaccacg ccatgtttgc gcagcatttg ggtaacagtg agaaccaggt ctgtggcggt
    34561 aataccttca cgtaattttc cggtaag
//
LOCUS       DAECOD010000034        34356 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.034, whole genome shotgun sequence.
ACCESSION   DAECOD010000034 DAECOD010000000
VERSION     DAECOD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.034"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..331)
                     /locus_tag="KKO15_001691"
     CDS             complement(<1..331)
                     /locus_tag="KKO15_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360507.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="KKO15_001692"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="KKO15_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBD6360508.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="KKO15_001693"
     CDS             complement(1692..2072)
                     /locus_tag="KKO15_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBD6360509.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="KKO15_001694"
     CDS             complement(2086..2502)
                     /locus_tag="KKO15_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360510.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KKO15_001695"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KKO15_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBD6360511.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="KKO15_001696"
     CDS             complement(3503..3970)
                     /locus_tag="KKO15_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBD6360512.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="KKO15_001697"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="KKO15_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBD6360513.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDASAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="KKO15_001698"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="KKO15_001698"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD6360514.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="KKO15_001699"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="KKO15_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBD6360515.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KKO15_001700"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KKO15_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBD6360516.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="KKO15_001701"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="KKO15_001701"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBD6360517.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="KKO15_001702"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="KKO15_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBD6360518.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="KKO15_001703"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="KKO15_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBD6360519.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KKO15_001704"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KKO15_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBD6360520.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="KKO15_001705"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="KKO15_001705"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBD6360521.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="KKO15_001706"
     CDS             complement(14625..15440)
                     /locus_tag="KKO15_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD6360522.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="KKO15_001707"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="KKO15_001707"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBD6360523.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="KKO15_001708"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="KKO15_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBD6360524.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="KKO15_001709"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="KKO15_001709"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBD6360525.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="KKO15_001710"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="KKO15_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBD6360526.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVYVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="KKO15_001711"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="KKO15_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBD6360527.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEARFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="KKO15_001712"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="KKO15_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBD6360528.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="KKO15_001713"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="KKO15_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBD6360529.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="KKO15_001714"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="KKO15_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBD6360530.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="KKO15_001715"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="KKO15_001715"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBD6360531.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KKO15_001716"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KKO15_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBD6360532.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="KKO15_001717"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="KKO15_001717"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBD6360533.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="KKO15_001718"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="KKO15_001718"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBD6360534.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KKO15_001719"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KKO15_001719"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBD6360535.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KKO15_001720"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KKO15_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBD6360536.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="KKO15_001721"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="KKO15_001721"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBD6360537.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="KKO15_001722"
     CDS             31574..32188
                     /locus_tag="KKO15_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBD6360538.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="KKO15_001723"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="KKO15_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBD6360539.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="KKO15_001724"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="KKO15_001724"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBD6360540.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8078 a         8682 c         9280 g         8316 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctagctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgtca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 cgtcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 ttttctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggtttatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgaggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgatagttt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgcatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcctagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgccgc cgggtc
//
LOCUS       DAECOD010000035        34281 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.035, whole genome shotgun sequence.
ACCESSION   DAECOD010000035 DAECOD010000000
VERSION     DAECOD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.035"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..578
                     /locus_tag="KKO15_001725"
     CDS             <1..578
                     /locus_tag="KKO15_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6360541.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="KKO15_001726"
     CDS             complement(575..1711)
                     /locus_tag="KKO15_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6360542.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="KKO15_001727"
     CDS             complement(1717..2040)
                     /locus_tag="KKO15_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD6360543.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="KKO15_001728"
     CDS             2223..2366
                     /locus_tag="KKO15_001728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360544.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KKO15_001729"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KKO15_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBD6360545.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5337)
                     /gene="yiaY"
                     /locus_tag="KKO15_001730"
     CDS             complement(4288..5337)
                     /gene="yiaY"
                     /locus_tag="KKO15_001730"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBD6360546.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVKEEDFAVLATNALKDACGFTNPI
                     QATHEEIVVIYRAAM"
     gene            complement(5527..7371)
                     /gene="selB"
                     /locus_tag="KKO15_001731"
     CDS             complement(5527..7371)
                     /gene="selB"
                     /locus_tag="KKO15_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBD6360547.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7368..8759)
                     /gene="selA"
                     /locus_tag="KKO15_001732"
     CDS             complement(7368..8759)
                     /gene="selA"
                     /locus_tag="KKO15_001732"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBD6360548.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEAIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8857..9465)
                     /locus_tag="KKO15_001733"
     CDS             complement(8857..9465)
                     /locus_tag="KKO15_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBD6360549.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9536..10621)
                     /locus_tag="KKO15_001734"
     CDS             complement(9536..10621)
                     /locus_tag="KKO15_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6360550.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10624..10986)
                     /locus_tag="KKO15_001735"
     CDS             complement(10624..10986)
                     /locus_tag="KKO15_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD6360551.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11523..13436
                     /gene="mtlA"
                     /locus_tag="KKO15_001736"
     CDS             11523..13436
                     /gene="mtlA"
                     /locus_tag="KKO15_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBD6360552.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13531..14679
                     /gene="mtlD"
                     /locus_tag="KKO15_001737"
     CDS             13531..14679
                     /gene="mtlD"
                     /locus_tag="KKO15_001737"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD6360553.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14679..15266
                     /gene="mtlR"
                     /locus_tag="KKO15_001738"
     CDS             14679..15266
                     /gene="mtlR"
                     /locus_tag="KKO15_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBD6360554.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15278..15487)
                     /locus_tag="KKO15_001739"
     CDS             complement(15278..15487)
                     /locus_tag="KKO15_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360555.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15771..16133
                     /locus_tag="KKO15_001740"
     CDS             15771..16133
                     /locus_tag="KKO15_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBD6360556.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16677..17360
                     /locus_tag="KKO15_001741"
     CDS             16677..17360
                     /locus_tag="KKO15_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD6360557.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17404..22066
                     /locus_tag="KKO15_001742"
                     /pseudo
     CDS             17404..22066
                     /locus_tag="KKO15_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22434..24089
                     /gene="lldP"
                     /locus_tag="KKO15_001743"
     CDS             22434..24089
                     /gene="lldP"
                     /locus_tag="KKO15_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBD6360558.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            24089..24865
                     /gene="lldR"
                     /locus_tag="KKO15_001744"
     CDS             24089..24865
                     /gene="lldR"
                     /locus_tag="KKO15_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBD6360559.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24862..26052
                     /gene="lldD"
                     /locus_tag="KKO15_001745"
     CDS             24862..26052
                     /gene="lldD"
                     /locus_tag="KKO15_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBD6360560.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26248..26721
                     /gene="trmL"
                     /locus_tag="KKO15_001746"
     CDS             26248..26721
                     /gene="trmL"
                     /locus_tag="KKO15_001746"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBD6360561.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26774..27595)
                     /gene="cysE"
                     /locus_tag="KKO15_001747"
     CDS             complement(26774..27595)
                     /gene="cysE"
                     /locus_tag="KKO15_001747"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBD6360562.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27675..28694)
                     /gene="gpsA"
                     /locus_tag="KKO15_001748"
     CDS             complement(27675..28694)
                     /gene="gpsA"
                     /locus_tag="KKO15_001748"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBD6360563.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28694..29161)
                     /gene="secB"
                     /locus_tag="KKO15_001749"
     CDS             complement(28694..29161)
                     /gene="secB"
                     /locus_tag="KKO15_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBD6360564.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29224..29475)
                     /gene="grxC"
                     /locus_tag="KKO15_001750"
     CDS             complement(29224..29475)
                     /gene="grxC"
                     /locus_tag="KKO15_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBD6360565.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29617..30048)
                     /locus_tag="KKO15_001751"
     CDS             complement(29617..30048)
                     /locus_tag="KKO15_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBD6360566.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30293..31837
                     /gene="gpmM"
                     /locus_tag="KKO15_001752"
                     /gene_synonym="pgmI"
     CDS             30293..31837
                     /gene="gpmM"
                     /locus_tag="KKO15_001752"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBD6360567.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31847..33130
                     /gene="envC"
                     /locus_tag="KKO15_001753"
     CDS             31847..33130
                     /gene="envC"
                     /locus_tag="KKO15_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBD6360568.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            33134..34093
                     /locus_tag="KKO15_001754"
     CDS             33134..34093
                     /locus_tag="KKO15_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBD6360569.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(34080..34281)
                     /locus_tag="KKO15_001755"
                     /pseudo
     CDS             complement(34080..34281)
                     /locus_tag="KKO15_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8528 a         8981 c         8743 g         8029 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt cacgcccatt gcagcggcac agtcacgcag
     4441 acgtgcagcg gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca
     4501 tacaccgtgt ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc
     4561 cagagaagca ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg
     4621 cgctttcgca ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac
     4681 ggctttcagt gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat
     4741 agcgtgcgtt aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat
     4801 cagagaggag tcattgacag aaagcagcgg agtcacgtgc ttatcaacaa tcgccatttt
     4861 gttatgacgc gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc
     4921 cgtggtattg atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta
     4981 atcgcgaata tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg
     5041 agaaccaccg cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc
     5101 tgcggcgacg ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat
     5161 attgcgttct tccagtgctt tttgcacatc acccgccata cctaatttcg ttaacatatt
     5221 gtcagtgaca attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt
     5281 caatgaatca gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac
     5341 acactcctga aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat
     5401 gatcattatt attaattatc aaaattaatc taataaaaag cacattgtat tcattaaaca
     5461 atgtgcttta tttaaaatca taagaaattt gtattaattt attttaaaaa ttaattaatc
     5521 atttccttat ttttccggaa ataataatgc gtcgcgcaat aaatgatcat ttccacgacg
     5581 acgcgtaaag ccaatgcggt caaaatattc cagaatctga attgccagct ttcggcctac
     5641 gcctaagcga tcgcggaaat ccgccgcgca ggttgaacca cactcctgat cgagatcgcg
     5701 gatcatattg gcaaattcga caatccgatc gttacggtaa taacgatctt tgacgatcgc
     5761 ggtaattatt ccttgctgcg ccgcctggcg tagcgtcagg cgcattgcct gctcgtcggt
     5821 acccgtctct tttgccagat cacgcaccca ccacggctcg tcaccaaaca gcggctctgc
     5881 tttttgccag atggcctgct gctcttcgct gaagcccgct ttgtgatccg gcagatgcag
     5941 ccaaccgtga tgactgagga tgtcgccgct ttcgcgcatc ttttcaatca gcaacagtac
     6001 tagcgcttca tcttccattg gcaacgccat acgtcgcaga cgttcgcgcc caggcccagg
     6061 ttcatcgcga tgttgctcat gataagtcgc taatgtgtcg agaattttcc gttgccagcg
     6121 agcggcaacc ggcgcattca acaagctata accagcctga atatagccag gctgttgcag
     6181 cagttcgcgc atcccttcgc cgttgagctg gcgcgcccag gcgaaatcag gaagattaac
     6241 cgcgccgcgt tgcaggtgaa cggctaacgc ttcgctatca ttctgtgccc tggcgaggga
     6301 agccagccat tgcagatact ctggtttacg tttaccgcga cgcggcgggt taagcatcac
     6361 gacgcgcgct ccggccagcg tgttgcgggc agagatatcg cgcaatacca ggcggtcgtt
     6421 atctgccagc cataacgggg tgtcgaagac cagttcagca aggttatctt ccagcagtga
     6481 aacgcgtccc gtgacgtggc tggcggcgtg gtgaatatgc agcggctgcc actgggtcag
     6541 cggtgcatgg gtttgaagct cgacaatcac ccgtgtgaac ggctctggcg gcacatcggc
     6601 aagcagccag tcgccacggt taatctgctc tttttccgca tcacccgcga tgtcaagcgc
     6661 gatacgctgc ccggcattgg cggtttctgt tggctggttt tgcgcatgca gcgcacgtac
     6721 acgcatcggt ttatttacac cagtcagcca gagtgaatcg cctaccttca cttccccgct
     6781 taacgccgta ccggtgacga ccagcccggc accttttacg gtaaatgcgc ggtcaatcgc
     6841 gaggcggaaa ctatgttggc tggcgtgctc gcgttccggc aactgaagca gatgctcgcg
     6901 cagggcatca attccccgac cttcggttgc tgcggtgata aacagttttg cctcagcaaa
     6961 accgtattcc cgcagaacct cctttacctg gcgttcaacc tcatcaacac gcgcttcgtc
     7021 cacgcgatcg gctttggtca gcgccactgt cagcatcggg ttaccggtca gctgcaaaat
     7081 cgccagatgc tcacgggtct gtgccatcac gccgtcatcg cacgccacca ccaacagcgc
     7141 gtgatcgata ccaccaacgc ccgccagcat gttggaaaga aacttttcat gaccgggaac
     7201 gtcgataaaa ccaggcacgc gaccatccgg ctgcggccag taggcatagc cgagatcgat
     7261 ggtcatgccg cgcttttttt cttccggcag acggtcagca tttacgccag taatcgcctg
     7321 caataaggtt gttttgccgt ggtcaacgtg tccggcagtc gcaataatca tttcaacaac
     7381 atctccaaaa accgttgctc atcttcaagg cagcgtaaat ccagccacaa tcgtccgtca
     7441 taaatacgac caatcaccgg cactggcaat tcacgccagc gggcggctaa tgactcaagg
     7501 tggctaccgc gtccatcatg gggtgtaaac gttaatgccg cgctcggcag gcgatcaacc
     7561 ggcagcgaac cactgccaat ctgcgaaaga catggcataa cctgtaccgc aaactccgcg
     7621 ccgtaatgtg cgacaagggg agcctgtaaa cgttgtgcct ggatttgaat ggcctctgcg
     7681 ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca gagcttcagg gtgtaaataa
     7741 agacgcaacg tggcttccag cgccgcgagg gtcattttat ccgcgcgtaa tgcacgcttc
     7801 agcgggtggc tttgcaggcg ggcgatcatc tcttttttac caacgataat cccggcctgc
     7861 ggcccgccta acaacttgtc gccggagaag ctcaccagac tgacgcccgc cgcaatcaac
     7921 tcctgcggca ttggctcttt cggcaaaccg tactggctaa gatcgaccag cgagccactg
     7981 cctaaatcag tcaccactgg aacatccagc tctttgccga gcgccaccag ttcagcttcg
     8041 tctatcgctt tggtgaaccc ctgaatgctg tagttactgg tatgtacttt catcaacagt
     8101 gcggtatttt cattcaccgc ctgacgataa tcattcgcgt gcgtgcggtt ggtggtccct
     8161 acttcgtgta gggtgcagcc tgcctgacgc ataacatcgg gaatacgaaa cgcgccgcca
     8221 atctccacca gttcgccgcg agataccacc acctctttcc cgctggcagt ggccgccaac
     8281 atcaataaca ccgccgccgc attgttattg acgatacagg catcttccgc ccccgtaata
     8341 cggcacagca gctgcgccag cgcccgatcg cgatgtccgc gtccggcgtc gtccagatcg
     8401 tactcgaggg tcactggcga acgcatagcc tgcgcaacgg cttccaccgc ggcttccgcc
     8461 tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg tcaggttgat caccggacgc
     8521 agcgcgctct gcgcctcttt cgtcaaccgg gcatcgactt cttgcgccca gttttcacac
     8581 cacgcaggca gcgtctggct gccacgaatc acttctcgcg cttcgtcgag catctgacgc
     8641 aacaattcca ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa ggagctatcg
     8701 cgcaataagc gatcaatagc cggaagttga ctatagaggg aacgcgtttc ggttgtcata
     8761 ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg cgtttaacat aaccacggat
     8821 tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa gcctttggcg gttcggtgcg
     8881 agcaaaactt tcgcggctaa acaggttttc gaccagtttg actaagtgtg ggcggtcgac
     8941 acaccaacct ggcgcaacgc ggcggaaatt gagatatccg acagcacagg caatggcgat
     9001 agtcgccaga ttgaccgtat cggttttgag tgtgccatcg accagatatc cttccagcac
     9061 atccagactg cggttgattt tctcccgctg gcgtaacaat tcatcttcag actgctgcgc
     9121 cgccggacgc gcctgttcac gcaccgatac cagcccggca tccataatgc catccgccag
     9181 cgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca tcgccggagc
     9241 gacattcatt aactcaatat attcagcgat gatcggcgaa tcaaaccagc attcgccctc
     9301 ttcggtcacc agcaccggca ctttccctaa cgggttaaat tgcgccacac cgttatccgc
     9361 gttatagggc agttcattaa taaattcgaa agttatgccc ttttctaaca acagaataga
     9421 aagtttgcgt acaaacgggc tggtgtagct accgacgagt ttcatgccga gtcctttgtg
     9481 cgaggaaaaa tatcagtatg gctcaggctc acgcaaaaca caggtgatat aagtctcaat
     9541 gatccaaata aagataatgc atccaactgg tcattcttag cagcactttc cgcatcaccg
     9601 aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg
     9661 catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc gtgccgggaa
     9721 cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc acgacaggta
     9781 aaatactagt caatttgccg tggaacacct gcccaggtag cgcgttaaac accacttccg
     9841 catcatcgcc aggtttcaga cgtaacagcg agttttgccg aaattgggcg acaatttgcc
     9901 gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct gcgtatgtac
     9961 ctgggcggat cagtacctga gtgacgtagc cattgcttgg tgcgcgaatg acagtctgct
    10021 caagattata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc gattgctcgc
    10081 cgttgaccat actgtcgagc tgactctgga tctgcgcctg ctccgccacc gagcctttca
    10141 ctaacgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg
    10201 gattcaccgc cgcctggctg cctttcaggt aacgttgata atttttaaac agacggtcgc
    10261 gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca cgcagcgtct
    10321 ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc gcctggtaac
    10381 gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta ttcttgtcag
    10441 tgacttcagt aacaattccc gtcacctgtg gcgtgatagg gatcgctatc actgcctttt
    10501 gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa
    10561 acacgcctcc cagcgccgcc gtcgccagcg ccacgtaagt taaaacaatc aatagatcca
    10621 taattagatc tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc
    10681 cagccgggaa attcggtcag aaagtgcggc gatttccggt tcagtcgctt tctcctgcga
    10741 cgcaacatgt gactgcatac cccagccacg ctccggttga tagagcgtcg cccagatcca
    10801 cagaaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg tatgaatagc
    10861 gtcggcatgg gggtgattgc gctttttggc aatcagataa gggatgtcat gtatcgcgat
    10921 gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag
    10981 aaacatatct gcctcggatt cacgtttatc agtgttgttt tttggctggc agccagaagg
    11041 gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga
    11101 ataattttta gaggtgatga gttgcttagt tacataacga ttgtatgacg aaggcataac
    11161 atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa
    11221 gattcaacaa accatcaaaa caaaaatgtg acattactca catttaaatg ccatttttag
    11281 cgaaaatcac cgccttgttt cttttttata caggcgtttt gtgatgagcg tcacgtcaat
    11341 tacctctcta ccccctatat ttatgtgact gatatcacac aaaagcccgt cgactggaca
    11401 gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca
    11461 cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt
    11521 ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg
    11581 tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa
    11641 cagggtggtt accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc
    11701 taccgctgct gatcggttat accggtggta agctggtagg cggcaaacgt ggtggcgtag
    11761 tcggtgccat taccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt
    11821 ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg
    11881 gtaagatcaa atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga
    11941 tgatcctcgc tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa
    12001 tgctggctgc gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct
    12061 ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc
    12121 cgctgggtat tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta
    12181 acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta
    12241 aacagtctgc gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact
    12301 tcccgtatgt gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg
    12361 gcgtgttcac gctgactatc ctgggcggtg gtctggtttc tccggcatct ccgggttcta
    12421 tccttgctgt actggcgatg acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt
    12481 gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga
    12541 aagaagaaga tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta
    12601 aaggcacatc tccgctatct gcgggcgatg tgactaacga cctgagccac gtacgtaaaa
    12661 tcatcgttgc ctgtgacgcc ggtatgggtt caagtgcgat gggcgcaggc gtgctgcgta
    12721 agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg atcaacaacc
    12781 tgccgccaga tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc
    12841 aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca
    12901 gcctaaccga acgtctcgtt gctgcccagc gccatactga aaacgaagtg aaagtgaaag
    12961 acagtctgaa agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga
    13021 acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc
    13081 agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa
    13141 aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga
    13201 aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg
    13261 gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc
    13321 acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc
    13381 tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca
    13441 atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
    13501 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
    13561 atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc
    13621 gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg
    13681 gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt
    13741 gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt
    13801 gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt
    13861 aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac cacgcagctg
    13921 aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agagcacgtt
    13981 ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
    14041 ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa
    14101 ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa
    14161 cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc
    14221 ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt
    14281 gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat
    14341 gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta
    14401 gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
    14461 ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt
    14521 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct
    14581 gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
    14641 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca
    14701 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggccg
    14761 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
    14821 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
    14881 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg
    14941 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
    15001 aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg
    15061 cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac
    15121 caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct
    15181 atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccaaaatca
    15241 gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg
    15301 gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg
    15361 gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct
    15421 ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
    15481 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt
    15541 ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc
    15601 tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
    15661 agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc
    15721 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
    15781 tcgaaaaaaa cgaaatcaag cgtctcagcg atcgcctgga cgccatccgc caccagcagg
    15841 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
    15901 cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag
    15961 cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag caggctgata
    16021 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
    16081 ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt taagcccctc
    16141 agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt caatgcgggg
    16201 actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc
    16261 tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat
    16321 atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg
    16381 tgaaatttta atcatcatat tcacaaaatc aattatctgt gaaatttttc ccagacatgg
    16441 tttctttcat atttgacatt tattaataat catcattaga atccacgaaa aataatatat
    16501 tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa
    16561 caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct caccaataat
    16621 acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga
    16681 acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat
    16741 gtgactacat tgaagaaggc aagccagaaa gtagtttact gaaacaacaa gaagaacata
    16801 ataataaaat tgtgctactt gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta
    16861 ttcaaaaaca acaaactggc ataataaaca gtacaaagac tttaactcac gtaattaaat
    16921 ccgttaaaga ccaacaaaat acttttattt ttaccgagtt taacccagca aaaacgaagt
    16981 atttcatcct gaacaacggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
    17041 cagagaatgg tagtgttatt catatctcac tggtcaactt attaagtaca cctatctcaa
    17101 acattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
    17161 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
    17221 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
    17281 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
    17341 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
    17401 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
    17461 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
    17521 ctggttgcag gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
    17581 ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa
    17641 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
    17701 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
    17761 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
    17821 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
    17881 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
    17941 ctggggcgta gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt
    18001 gccagcgcag cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact
    18061 gctattggca ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc
    18121 cttgccgata aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat
    18181 gcaatcgcca taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat
    18241 ggtagccagt cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt
    18301 gataagagtc ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg
    18361 ggcgcagatt ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa
    18421 cgcaagatcg ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat
    18481 ggttcacagc tttatgccat tagcgactcg gtagcaaaaa aggcttggag gaggggctgc
    18541 agtagatgtt gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa
    18601 aaataacgta ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac
    18661 caaaggcaaa tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga
    18721 tgttgcggat ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa
    18781 agctaccaat gacgatgtcg aagccaacac caccaatatc accaacctga cggattccgt
    18841 tggtgacctt caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc
    18901 tcacggccag gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga
    18961 cagcactgat gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa
    19021 taccaccaat atcgccaata acacttccaa tattgccact aacaccacca acatctctaa
    19081 cctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg
    19141 tgtattcacg gcagctcatg gcaccgagac caccagcaaa ataaccaacg ttaaagatgg
    19201 cgacctgacg actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga
    19261 tgccgtggcg acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac
    19321 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt
    19381 cactgcagct catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt
    19441 cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat
    19501 cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac
    19561 ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc
    19621 ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag
    19681 tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat
    19741 ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt
    19801 tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac
    19861 gtacaccatt gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac
    19921 cactcctgac gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc
    19981 cgctcacgga aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc
    20041 tgctgccagc agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc
    20101 tgatgcgctg ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta
    20161 caccattgcg aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa
    20221 cgccctgctg tgggataaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga
    20281 cggtaaagcc agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga
    20341 tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat
    20401 tatcaatcag ctcgctggta acaccgacgc aacctatatc gaagaaaatg gcgctggtat
    20461 caactacgtt cgtactaacg acaacgattt agcctttaac gatgcaagcg cctctggtgt
    20521 cggcgctaca gctgtaggtt ataacgctgt cgcgtctggt gccagcagcg tagccattgg
    20581 tcagaacagc agcagcaccg ttgataccgg tattgcgctg ggtagcagct ccgtttccag
    20641 ccgtgtgatt gccaaaggtt ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg
    20701 ttatgacacc actgacggcg aactgctagg tgcattgtca attggtgatg acggtaaata
    20761 ccgccaaatc atcaacgtag ctgatggttc agaagcccat gacgccgtta cggttcgcca
    20821 gttgcagaac gctattggag cggtcgccac tacgccaacc aagtactttc acgccaactc
    20881 aacggcagaa gactcactgg ccgttggtga agactcgctg gcaatgggtg cgaaaactgt
    20941 cgttaatggt aatgcaggta ttggtatcgg tttgaacact ctggttctgg ctgatgcgat
    21001 caacggcatt gctatcggca gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg
    21061 taacggttct cagaccaccc gtggtgcaca gactggctac accgcctaca acatggacgc
    21121 accgcagaac tctgtaggtg agttttctgt cggtagtgaa gacggtcaac gtcagatcac
    21181 aaacgtcgca gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac
    21241 tgatgagcgc gtagcgcaaa atacccagag cattactaac ctgaacaatc aggtcactaa
    21301 tctggatact cgcgttacta atatcgaaaa cggtattggc gacattgtca ccaccggtag
    21361 caccaagtac ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag
    21421 cgttgctatt ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg
    21481 ttccgttgca gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat
    21541 caccaacgta gctgccagtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc
    21601 ttctgaagca ggcggagtgc gctacgacac caaagctgat ggttctatcg actatagcaa
    21661 tatcaccctc ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt
    21721 caacaacaac gacgcggtga actacgcgca gttgaagcaa agcgcgcagg aaacgaagca
    21781 atacaccgat cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt
    21841 gagcggtggt atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg
    21901 tgccagcatg gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg
    21961 tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag
    22021 ccagggtgaa tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt
    22081 agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag
    22141 gcgctccggg tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca
    22201 gacatctccc cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca
    22261 caattcccca attaaaactc acatcaatat tgccaataca taacatttag ttaaccattc
    22321 attgtcatta tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag
    22381 gagatgagca acagactcat tacacgatgt gcgtggactc taggagacct gcaatgaatc
    22441 tctggcaaca aaactacgat cccgccggga atatctggct ttccagtctg atagcatcgc
    22501 ttcccatcct gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg
    22561 cctcatggac ggtagcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg
    22621 ctaacgcgct ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga
    22681 tcattattgc agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca
    22741 ttcgctcgtc tattctttcg ataacccctg accagcgtct gcaaatgctg atcgtcggtt
    22801 tctgtttcgg cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg
    22861 ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc cgccggactg tgcctgattg
    22921 ttaacaccgc gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacaag
    22981 taacaggcat cgacagcttt gagattggcc agatggtggg gcgtcagcta ccgtttatga
    23041 ccattatcgt gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga
    23101 cgtggcctgc ggtcgtggtt gcaggcggct cgtttgccat cgctcagtac cttagctcta
    23161 acttcattgg gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga
    23221 cgctgttcct caaacgctgg caaccagttc gcgtattccg cttcggtgac ttgggggcgt
    23281 cacaggttga tatgacgctg gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct
    23341 ggacaccgtt cctgttcctg accgctaccg taacactgtg gagcatcccg ccgtttaaag
    23401 ccctgttcgc atcgggtggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc
    23461 tcaataaact ggttgcccgt atgccaccag tggtcagcga ggctacagcc tatgccgccg
    23521 tgtttaagtt tgactggttc tctgccaccg gcaccgccat tctgtttgct gcactgctct
    23581 cgattgtctg gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacgctga
    23641 aagaactggc tctacccatc tactccatcg gcatggtgct ggcgttcgcc tttatctcga
    23701 actattccgg actgtcatca acactggcac tggcactggc gcacaccggt catgcattca
    23761 ccttcttctc gccgttcctc ggctggctgg gggtattcct gaccgggtcg gatacctcat
    23821 ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc
    23881 tgttgctggt tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat
    23941 ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct
    24001 ttactgtcaa acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg
    24061 cttatgtctt aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga
    24121 ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt
    24181 gcccgctgag cgccaactgg cgatgcaact cggcgtatca cgtaattcac tacgcgaggc
    24241 gctggcaaaa ctggtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat
    24301 tcgctggcgt catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat
    24361 ggccgatgat ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag
    24421 caccgcatgg catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg
    24481 ctttgaagca acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca
    24541 tctggcgatt gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt
    24601 cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt
    24661 tttttcacaa ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc
    24721 cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt gttcacacca ccatgaaacg
    24781 attcgatgaa gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga
    24841 gcattcgagg gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg
    24901 caacgcattc tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac
    24961 acgctgcgcc gcaacgtgga agatttgtca gaagtggcac tgcgccagcg tattctgaaa
    25021 aacatgtccg acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggta
    25081 gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc
    25141 aaagcggcgg acgcgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt
    25201 gaagaagtcg cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat
    25261 cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt
    25321 ttcaccgtgg atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc
    25381 ggcccaaatg cggcaatgcg ccgctacttg caggcggtga cgcatccgca atgggcgtgg
    25441 gatgtgggcc tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa
    25501 ccgaccggac tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca
    25561 tggaaagacc ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc
    25621 ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct
    25681 aaccacggtg gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt
    25741 gcagatgcgg tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt
    25801 gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg
    25861 tatgcgctgg caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa
    25921 gagatgaaag tggcgatgac gctgactggc gcgaaatcga tcagtgaaat tacgcaagat
    25981 tcgctggtgc aggggctggg taaagagttg cctacggcac tggctccgat ggcgaaaggg
    26041 aatgcggcat agtcgtttgc gccccctcac cctaaccctc tccctcaggg agaggggacc
    26101 gttcggcgct gtatgtactc cctcactctg aaacgacacc gcactctttt tttctccctc
    26161 gcccctccgg ggagggccgg ggtgagggga aaaggccgca cggttcacca attttctgct
    26221 atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt
    26281 ccgccaaata ctggcaacat catccgtctt tgcgctaata ccggctttcg tctacatatc
    26341 atcgaaccga tgggatttgc ctgggacgac aagcgcctgc gccgcgcggg gctggactat
    26401 cacgagttta ccgccgttac gcgtcatcat gactatcgcg cgttcctcga agcagaaaat
    26461 ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat
    26521 caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt
    26581 gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag ccgcagcatg
    26641 aatctgtcca atgcggtgtc ggtagtggtg tatgaagcct ggcggcagct gggatatccg
    26701 ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc gcatccggca
    26761 cgatcacagg acattagatc ccatccccat actcaaatgt atggttaata ccgttgaaat
    26821 gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata cgaaccggaa
    26881 cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct
    26941 tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca atcatcacac
    27001 cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca agcgtcacag
    27061 attgcagaat cgatacgtcg ttttcaatta ccgccgtttc acccacaacg atgcccgtgg
    27121 cgtggtcgag cataatgcca cggccaattt ttgctgccgg gtgaatatcg acctggaacg
    27181 tcacagaaac ctggttttgc agaaagattg ccaatgcgcg acgcccctga ttccacaacc
    27241 agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac aacggggttg
    27301 agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag gccgcagagg
    27361 cgatcatttc cgggtcagcg gcgtaggctt cttcaaccac ttcacggata gcaatagcag
    27421 gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt
    27481 cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg
    27541 ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac gacattgctt
    27601 actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca
    27661 aaggttccct ggggttagtg actgctgcgc tcgtccttgc gtgcacgacc taataatgtc
    27721 aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt
    27781 ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct
    27841 tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc
    27901 ataccaaaac ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc
    27961 cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcaccaag acgcgacatt
    28021 tcagccagcc cacgggtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtca
    28081 gacatccccg cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacgccaatg
    28141 aaatccgggt tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg ctggagatca
    28201 tcggcaaagg tctgatcggt cgaggccagc gaaatagctg tcggtaaacc tgccgccagt
    28261 tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca
    28321 cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt cgcccacacc
    28381 agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg
    28441 ctgggtacga cgacgagaat attacgactg gctgccagcg cagtggcgag atcgctttca
    28501 agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt
    28561 tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt
    28621 gccagggtaa tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca
    28681 ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca
    28741 aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac
    28801 cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt
    28861 atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc
    28921 cctgctgaac ttcacacagg aacgcggttt cttcgcccaa ggaggccgtt acggtaacac
    28981 gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc agatcaagtt
    29041 taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga
    29101 tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt tgttctgaca
    29161 tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatac caacgttcac
    29221 acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca agtcatcaca
    29281 gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcaac cgctgcgttt
    29341 gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgga aactcacgcc
    29401 cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt tggtatagat
    29461 ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat gttgcagggc
    29521 ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg gcctaattgc
    29581 agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa
    29641 gttttcgcca gcccagccag caacgccctc tttcagcacg aatacttgcg caaaaccagc
    29701 tttcgtcagc gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta ccacgataac
    29761 cgatttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa
    29821 cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac
    29881 aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac
    29941 tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc
    30001 gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa
    30061 ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt gcggcaaata
    30121 cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc
    30181 tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat tttatcgatt
    30241 cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg caatgtcggt
    30301 ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg aagaacagca
    30361 ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc
    30421 gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc agatgggtaa
    30481 ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg acctgactcg
    30541 tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctgg ctggcgcggt
    30601 acataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct ccgcaggcgg
    30661 cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg aacgcggcgc
    30721 agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc gcagtgctga
    30781 atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc
    30841 catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc
    30901 ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg ttgctggttt
    30961 gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc
    31021 agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca tgaacttccg
    31081 tgctgaccgc gcgcgtgaaa tcactcgtgc attcgtgaac gctgatttcg atggcttcgc
    31141 gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa
    31201 aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa
    31261 aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg ttactttctt
    31321 cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga tcaactcacc
    31381 gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga ccgaaaaact
    31441 ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc cgaacggcga
    31501 catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag cactggatca
    31561 ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc tgatcaccgc
    31621 tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac acacggcaca
    31681 caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag cggttgcagg
    31741 cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg aaatcccgca
    31801 agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga ggggaaaggc
    31861 gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta
    31921 cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg
    31981 tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg tacgccaaaa
    32041 gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag cgatctctga
    32101 agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata aacagattga
    32161 tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct
    32221 cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc agctaattct
    32281 cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct atctcaacca
    32341 ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtca ccatgcagcg
    32401 tgctgaactg gaagagaaac aaagcgagca acaaacgctg ttatatgaac agcgcgccca
    32461 acaggcgaaa ctgactcagg ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc
    32521 ttccatccag caaggtcagc agcagctaag tgaactgcgt gccaacgaat cccgtctgcg
    32581 taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga
    32641 ggcccaggcg gttcgcgagc gccagaaaga agcgacgcgc aaaggcacca cctacaaacc
    32701 gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca
    32761 ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggcgagcagc tacagggtga
    32821 attacgctgg aaagggatgg ttatcggtgc ctctgaaggt actgaagtta aagcaattgc
    32881 cgacggtcga gtgattctgg ctgactggct gcaaggttac ggtctggtgg tggtggttga
    32941 gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg tgagcgttgg
    33001 ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg gtcagggtcg
    33061 gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac agccgtggtt
    33121 gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg ctctgttggc
    33181 gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt ttgggtatcg
    33241 cccgcacaac gaaaaccagg tgctggcgat gccttccgct atctccgtcg ctgtattacc
    33301 cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcggtc acgaagtgtt
    33361 gattcatctc ccgatggcac cgttgagtaa acagccgctg gagaaaaata cgctacgccc
    33421 ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata acgtgcccta
    33481 tgccgtgggg atcaacaacc acatgggcag caagatgacc tctaacctgt ttggtatgca
    33541 gaaagtgatg caggcgctgg agcgttacaa tctttacttc ctcgacagcg taaccatcgg
    33601 taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca aacggaaggt
    33661 gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc gcgcaattga
    33721 tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt caacggtacg
    33781 cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga aagccagcag
    33841 cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg tgcctgatgc
    33901 accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt
    33961 ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca cgctgatcgt
    34021 ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga agtttaacgc
    34081 tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat
    34141 tgccataagc ggaaagacca cagtaacgcc tggtaaccga ccttcacgct gcgcacattg
    34201 gtaatcaggc gcttatacat acccggaagt aaatatctcg gcaatcatcc gttggcgggt
    34261 aagaatatcc ggctctttgc g
//
LOCUS       DAECOD010000036        33781 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.036, whole genome shotgun sequence.
ACCESSION   DAECOD010000036 DAECOD010000000
VERSION     DAECOD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.036"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(183..659)
                     /locus_tag="KKO15_001756"
     CDS             complement(183..659)
                     /locus_tag="KKO15_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBD6360570.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(718..2007)
                     /gene="bioA"
                     /locus_tag="KKO15_001757"
     CDS             complement(718..2007)
                     /gene="bioA"
                     /locus_tag="KKO15_001757"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBD6360571.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QMAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2094..3134
                     /gene="bioB"
                     /locus_tag="KKO15_001758"
     CDS             2094..3134
                     /gene="bioB"
                     /locus_tag="KKO15_001758"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBD6360572.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3131..4285
                     /gene="bioF"
                     /locus_tag="KKO15_001759"
     CDS             3131..4285
                     /gene="bioF"
                     /locus_tag="KKO15_001759"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBD6360573.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4272..5027
                     /gene="bioC"
                     /locus_tag="KKO15_001760"
     CDS             4272..5027
                     /gene="bioC"
                     /locus_tag="KKO15_001760"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBD6360574.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5020..5697
                     /gene="bioD"
                     /locus_tag="KKO15_001761"
     CDS             5020..5697
                     /gene="bioD"
                     /locus_tag="KKO15_001761"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD6360575.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6276..8297
                     /gene="uvrB"
                     /locus_tag="KKO15_001762"
     CDS             6276..8297
                     /gene="uvrB"
                     /locus_tag="KKO15_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBD6360576.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8489..9397)
                     /gene="yvcK"
                     /locus_tag="KKO15_001763"
     CDS             complement(8489..9397)
                     /gene="yvcK"
                     /locus_tag="KKO15_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBD6360577.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      9662..9806
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9794..10783
                     /gene="moaA"
                     /locus_tag="KKO15_001764"
     CDS             9794..10783
                     /gene="moaA"
                     /locus_tag="KKO15_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBD6360578.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            10805..11317
                     /gene="moaB"
                     /locus_tag="KKO15_001765"
     CDS             10805..11317
                     /gene="moaB"
                     /locus_tag="KKO15_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBD6360579.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11320..11805
                     /gene="moaC"
                     /locus_tag="KKO15_001766"
     CDS             11320..11805
                     /gene="moaC"
                     /locus_tag="KKO15_001766"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBD6360580.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            11798..12043
                     /gene="moaD"
                     /locus_tag="KKO15_001767"
     CDS             11798..12043
                     /gene="moaD"
                     /locus_tag="KKO15_001767"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBD6360581.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12045..12497
                     /gene="moaE"
                     /locus_tag="KKO15_001768"
     CDS             12045..12497
                     /gene="moaE"
                     /locus_tag="KKO15_001768"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBD6360582.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            12634..13338
                     /locus_tag="KKO15_001769"
     CDS             12634..13338
                     /locus_tag="KKO15_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD6360583.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13543..14256
                     /locus_tag="KKO15_001770"
     CDS             13543..14256
                     /locus_tag="KKO15_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD6360584.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(14292..15248)
                     /locus_tag="KKO15_001771"
     CDS             complement(14292..15248)
                     /locus_tag="KKO15_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBD6360585.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(15248..16489)
                     /gene="clsB"
                     /locus_tag="KKO15_001772"
     CDS             complement(15248..16489)
                     /gene="clsB"
                     /locus_tag="KKO15_001772"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBD6360586.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(16486..17247)
                     /locus_tag="KKO15_001773"
     CDS             complement(16486..17247)
                     /locus_tag="KKO15_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD6360587.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17380..17790
                     /locus_tag="KKO15_001774"
     CDS             17380..17790
                     /locus_tag="KKO15_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBD6360588.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(17752..18858)
                     /locus_tag="KKO15_001775"
     CDS             complement(17752..18858)
                     /locus_tag="KKO15_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6360589.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(18869..20002)
                     /locus_tag="KKO15_001776"
     CDS             complement(18869..20002)
                     /locus_tag="KKO15_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6360590.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(19995..21731)
                     /locus_tag="KKO15_001777"
     CDS             complement(19995..21731)
                     /locus_tag="KKO15_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6360591.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(21724..22719)
                     /gene="hlyD"
                     /locus_tag="KKO15_001778"
     CDS             complement(21724..22719)
                     /gene="hlyD"
                     /locus_tag="KKO15_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBD6360592.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(22722..23393)
                     /gene="cecR"
                     /locus_tag="KKO15_001779"
     CDS             complement(22722..23393)
                     /gene="cecR"
                     /locus_tag="KKO15_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBD6360593.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            23622..24809
                     /gene="rhlE"
                     /locus_tag="KKO15_001780"
                     /pseudo
     CDS             23622..24809
                     /gene="rhlE"
                     /locus_tag="KKO15_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(25016..25498)
                     /gene="ybiA"
                     /locus_tag="KKO15_001781"
     CDS             complement(25016..25498)
                     /gene="ybiA"
                     /locus_tag="KKO15_001781"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBD6360594.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            25618..27768
                     /gene="dinG"
                     /locus_tag="KKO15_001782"
     CDS             25618..27768
                     /gene="dinG"
                     /locus_tag="KKO15_001782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBD6360595.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            27796..28758
                     /gene="ybiB"
                     /locus_tag="KKO15_001783"
     CDS             27796..28758
                     /gene="ybiB"
                     /locus_tag="KKO15_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBD6360596.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            28899..29984
                     /gene="hcxB"
                     /locus_tag="KKO15_001784"
     CDS             28899..29984
                     /gene="hcxB"
                     /locus_tag="KKO15_001784"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBD6360597.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(30213..30473)
                     /locus_tag="KKO15_001785"
     CDS             complement(30213..30473)
                     /locus_tag="KKO15_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6360598.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(30738..31004)
                     /gene="ybiI"
                     /locus_tag="KKO15_001786"
     CDS             complement(30738..31004)
                     /gene="ybiI"
                     /locus_tag="KKO15_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBD6360599.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(31078..31755)
                     /gene="ybiX"
                     /locus_tag="KKO15_001787"
     CDS             complement(31078..31755)
                     /gene="ybiX"
                     /locus_tag="KKO15_001787"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBD6360600.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(31797..33781)
                     /locus_tag="KKO15_001788"
                     /pseudo
     CDS             complement(31797..33781)
                     /locus_tag="KKO15_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         7954 a         8765 c         9130 g         7932 t
ORIGIN      
        1 agtattatgc agtctatttt ttaagctaaa tgtattataa tgcattgata ttaatgattt
       61 ttactgtttc tcggtcagct tttttatact aacttgagcg aaacgggaag gtaaaaagac
      121 aaaaagttgt ttttaatacc tttaagtgat accagatggc attgcgccat ctggcagagt
      181 gattaactaa acatcgcggt aatcgatgcg cttgccagag agtggaaatg aacgttaaac
      241 ccgaccatcg cgccgctggc tccttcatcg acatcaatac gctctacatc cagcgcgtga
      301 acggtaaaaa tgtagcgatg ggtttcgcct ttcggcggcg ctgcgccatc gtatccggtt
      361 ttaccaaaat cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag accagagcca
      421 aacccttgcg gtaatacgcg ggtatcagcg ggtaaattaa caactaccca gtgccaccag
      481 ccggagccgg ttggcgcatc cgggtcgtag caggtgacaa caaaactttt cgttcccgca
      541 ggaacatcat cccacgccag atgcggtgaa atattatcgc catcgtaacc catgccgtta
      601 aagacatgac gatgcggcaa cttatcgcca tcgcgcagat cgttactgat gagtttcatg
      661 aaccctcctt tcttgtttgc agaaagtgta gccagaaacc ctcacgctga cttcccgtta
      721 ttggcaaaaa aatgtttcat cctgtaccgc gcggttaacc gctgcggtca gacgctgcaa
      781 ctgttgcggg agaataatat agggcggcat caggtaaatc agtttgccaa aaggccggat
      841 ccataccccc tgttcgacaa agaatttttg cagcgccgcc atattcaccg gacgagtggt
      901 ttcgaccaca ccgattgccc ccagtacgcg cacatcggca accatttcgg catcacgggc
      961 tggtgccagt tgctcgcgca gctgcacttc aatagccgcc atctgctgct gccattcgcc
     1021 ggattcgata atcgctaggc tggcgtttgc tgccgcgcag gccagcggat tgcccataaa
     1081 agttggccca tgcataaagc agccggcttc gccgttactg atggtttctg caacctcgcg
     1141 cgtggtaagt gtggcggaaa gggtcattgt gccgccggtt aaggctttac cgaggcacaa
     1201 aatgtccggc gcgatttctg catattcaca ggcaaacagt ttgccggtac gaccaaatcc
     1261 ggtggcgatc tcgtcggcaa tcagcaagat accttcgcga tcgcacattt tgcggattcg
     1321 ttttaaccat tccggatggt acatgcgcat cccgcctgcg ccctggacaa tcggctcaat
     1381 gatcaccgct gcgatttcat gacgatgcgc cgccatcagg cgggcaaaac caaccatatc
     1441 gcgttcatcc cattcaccat ccatgcggct ttgcggggcg ggagcaaaca agttttctgg
     1501 cagatagcct ttccacaggc tgtgcattga gttgtccgga tcgcacaccg acatcgcgcc
     1561 aaaggtatcg ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg cttcgccttt
     1621 ggcttgccag tactgcaacg ccattttcat cgccacttcc accgctacgg aaccggagtc
     1681 cgcgagaaaa acgcactcca gcggttgcgg cgtcattgcc accagtttgc ggcatagctc
     1741 gatggcgggc gcatgggtga taccgccaaa catcacatgc gacatggcat caatttgcgt
     1801 cttcatcgcc gcattaagct gcgggtgatt gtagccgtgg atcgccgccc accaggacga
     1861 cataccgtca accaggcgtc tgccgtcaga caaaattagc tcgcaacctt cggcgctcgc
     1921 caccggataa accggcagag gggaggtcat ggatgtgtat gggtgccaga tatggcgttg
     1981 gtcaaaggca agatcgtccg ttgtcataat cgacttgtaa accaaattga aaagatttag
     2041 gtttacaagc ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc cccatggctc
     2101 accgcccacg ctggacattg tcgcaagtca cagaattatt tgaaaaaccg ttgctggatc
     2161 tgctgtttga agcgcagcag gtgcatcgcc agcatttcga acctcgtcag gtgcaggtca
     2221 gcacgttgct gtcgattaag accggagctt gtccggaaga ttgcaaatac tgcccgcaaa
     2281 gctcgcgcta caaaaccggg ctggaagccg agcggttgat ggaagttgaa caggtgctgg
     2341 agtcggcgcg caaagcgaaa gcggcaggat cgacgcgctt ctgtatgggc gcggcatgga
     2401 agaatcccca cgaacgcgat atgccgtacc tggaacaaat ggtgcagggg gtaaaagcga
     2461 tggggctgga ggcgtgtatg acgctgggca cgttgagtga atctcaggcg cagcgcctcg
     2521 cgaacgccgg gctggattac tacaaccaca acctggacac ctcgccggag ttttacggca
     2581 atatcatcac cacacgcact tatcaggaac gcctcgatac gctggaaaaa gtgcgcgatg
     2641 ccgggatcaa agtctgctca ggtggcattg tgggcttagg cgaaacggta aaagatcgcg
     2701 ccggattatt gctgcaactg gcaaacctgc cgacgccgcc ggaaagcgta ccaatcaaca
     2761 tgctggtgaa ggtgaaaggc acgccgcttg ccgataacga tgacgttgat gcctttgatt
     2821 ttattcgcac cattgccgta gcgcggatca tgatgccgac ctcttacgtg cgcctttctg
     2881 ccggacgcga gcagatgaac gaacagactc aggcgatgtg ctttatggca ggcgcaaact
     2941 cgattttcta cggttgcaaa ctgctgacca cgccgaatcc ggaagaagat aaagacctgc
     3001 aactgttccg caaactgggg ctaaatccgc agcaaactgc cgtgctggca ggggataacg
     3061 aacaacagca acgtcttgga caggcgctga tgaccccgga caccgacgaa tattacaacg
     3121 cggcagcatt atgagctggc aggagaaaat caacgcggcg ctcgatgcgc ggcgtgctgc
     3181 cgatgccctg cgtcgccgtt atccggtggc gcaaggagcc ggacgctggc tggtggcgga
     3241 tgatcgccag tatctgaact tttccagtaa cgattattta ggtttaagcc atcatccgca
     3301 aattatccgt gcctggaagc agggggcgga gcaatttggc atcggtagcg gcggctccgg
     3361 tcacgtcagc ggttatagcg tggtgcatca ggcgctggaa gaagaactgg ccgagtggct
     3421 tggctattcg cgggcgctgc tgtttatctc tggttttgcc gcgaatcagg cagtcattgc
     3481 cgcgatgatg gcgaaagagg accgtattgt tgccgaccgg cttagccatg cctcattgct
     3541 ggaggctgcc agtttaagcc cgtcgcagct tcgccgtttt actcataacg atgtcgctca
     3601 tctggcgcga ctgcttgctt ccccctgtcc ggggcagcaa ctggtggtga cagaaggcgt
     3661 gttcagcatg gacggcgata gcgcgccact ggcggaaatc cagcaggtaa cgcaacagca
     3721 caatggctgg ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg
     3781 cggcagctgc tggctgcaaa aggtaaaacc agaattgctg gtagtgactt ttggcaaagg
     3841 atttggcgtc agtggggctg cggtgctttg ctcaagtacg gtggcggatt atctgctgca
     3901 attcgcccgc caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg
     3961 tgcgtcgctg gcggtcattc gccgtgatga gggggatgca cggcgcgaaa aactggtgtc
     4021 actcattgcg cgttttcgtg ccggagtaca ggatttgccg tttacgctgg ctgattcatg
     4081 cagcgccatc cagccattga tcgtcggtga taacagccgt gcgttacaac tggcagaaaa
     4141 actgcgccag caaggctgct gggtcacggc gattcgcccg ccaaccgtac ccgctggtac
     4201 tgcgcgactg cgcttaacgc taaccgctgc gcatgaaatg caggatatcg accgtctgct
     4261 ggaggtgctg catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag
     4321 ccgcacacta tgagcaacat gcagatctac agcgccagag tgctgacgcc ttactggcaa
     4381 tgcttccaca gcgtaaatac acccacgtac tggacgcggg ttgtggacct ggctggatga
     4441 gccgccactg gcgggaacgt cacgcgcagg tgacggcgtt agatctctcg ccgccaatgc
     4501 ttgcgcaagc ccgccataaa gatgccgcag atcattatct ggcaggagat atcgaatccc
     4561 tgccgttagc gactgcgacg ttcgatcttg catggagcaa tctcgcggtg cagtggtgcg
     4621 gtaatttatc cacggcactc cgcgagctgt atcgggtggt gcgccccgga ggcgtggtcg
     4681 cgtttaccac gctggtgcag ggatcgttac ccgaactgca tcaggcgcgg caggcggtgg
     4741 acgagcgtcc gcatgctaat cgctttttac cgccagatga aatcgaagag tcgctgaacg
     4801 gcgtgcgtta tcaacatcat attcagctca tcacactatg gtttgatgat gcgctcagtg
     4861 ccatgcgttc gctgaaaggc atcggtgcta cgcatcttca tgaagggcgc gacccgcgaa
     4921 tattaacgcg ttcgcagttg cagcgattgc aactggcctg gccgcaacaa caggggcgat
     4981 atcctctgac gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc
     5041 accggaacgg ataccgaagt ggggaaaacc gtcgccagtt gtgcactttt acaagccgcg
     5101 aaggcagtag gttatcggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc
     5161 ccggaaggct tacgtaatag tgacgcgctg gcgttacagc acaacagcag cctgcagctg
     5221 gattacgcaa cggtaaatcc ttacaccttc gcagaaccca cctcgccgca catcatcagc
     5281 gcccaagagg gcaggccgat agaatcattg gtaatgagcg ccggattacg cgcgcttgaa
     5341 caacaggctg actgggtgtt agtggaaggt gctggcggct ggtttacgcc gctttctgac
     5401 actttcactt ttgcagattg ggtaacacag gaacaactgc cggtgatact ggtagttggt
     5461 gtgaaactcg gctgtattaa tcacgcgatg ttgactgcgc aggcaataca acacgccgga
     5521 ctgactctgg cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg tcacgctgaa
     5581 tatatgacca cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt
     5641 gcagaaaatc cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca
     5701 ttctgtattt ggttaaattg cgagcgagat cgcgtcttcg attgactgca atttaaccaa
     5761 ttaaattcta aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc gaatgtggga
     5821 attgcccagg cggcggggga taggggctgg agacagttat ccactattcc tgtggataac
     5881 catgtgtatt agagttagaa aacacgaggc aagcgagaga atacgcggct tgcacgcgaa
     5941 ttggcgttaa agacggctca aagaaatatc ttttattttt taactggtta gataaatgca
     6001 atggcagtca ctgaacaggc atctcttgcc ataaaactgt catcactcat cttgacaaat
     6061 gttaaaaaag ccgttgcttt ggggataacc cggtaaggct ggagttttat ctcgccacag
     6121 agtaaatttt gctcatgatt gacagcggag tttacgctgt atcagaaata ttatggtgat
     6181 gaactgtttt tttatccagt ataatttgtt ggcataatta agtacgacga gtaaaattac
     6241 atacctgccc gcccaactcc ttcaggtagc gactcatgag taaaccgttc aaactgaatt
     6301 ccgcttttaa accttctggc gatcagccag aggcgattcg acgtctcgaa gaggggctgg
     6361 aagatggcct ggcgcaccag acgttacttg gcgtgactgg ctccgggaaa accttcacca
     6421 ttgccaatgt cattgctgac cttcagcgcc caaccatggt acttgcgccc aacaaaacgc
     6481 tggcggccca gctgtatggc gaaatgaaag agttcttccc ggaaaacgcg gtggagtatt
     6541 tcgtctccta ctacgactac tatcagccgg aagcctatgt accgagttcc gacaccttca
     6601 ttgagaaaga tgcctcggtt aacgaacata ttgagcagat gcgtttgtcc gccaccaaag
     6661 cgatgctgga gcggcgtgat gtgattgtag tggcgtctgt ttccgcgatt tatggtctgg
     6721 gcgatcctga tttatatctc aagatgatgc tccatctcac ggtcggtatg attatcgatc
     6781 agcgcgcgat tctgcgccga ctggcggagc tgcaatacgc tcgtaatgat caagcattcc
     6841 agcgtggtac tttccgcgtt cgtggcgagg tgatagatat cttcccggca gaatcggatg
     6901 acattgcact tcgcgtggaa ctgtttgacg aggaagtgga acgattgtcg ttatttgacc
     6961 cgctgaccgg gcagattgtt tccactattc cacgttttac catctacccg aaaacgcact
     7021 acgtcacacc gcgcgagcgc atcgtacagg cgatggagga gatcaaagaa gaactggccg
     7081 ccagacgcaa agtgctgttg gaaaacaaca aactgctgga agagcagcgg ctgacccagc
     7141 gtacccagtt tgatctggag atgatggacg agctgggcta ctgttcgggg attgaaaact
     7201 actcgcgctt cctctccggt cgtggaccgg gtgagccacc gccgacgctg tttgattacc
     7261 tgcctgccga tgggctgctg gtcgtcgatg aatctcacgt caccattcca caaattggcg
     7321 gcatgtatcg cggtgaccgg gcgcgtaaag agacactggt ggagtacggc ttccgcctgc
     7381 catcagcgct ggataaccgt ccgcttaagt ttgaagagtt cgaagcatta gcgccgcaaa
     7441 ccatctatgt ttcggcgacg ccgggtaatt acgagctgga aaaatccggc ggcgatgtgg
     7501 tggatcaggt ggtgcgtcca accggattgc ttgacccgat tatcgaagtg cggccggtgg
     7561 cgacacaggt tgatgatctt ctttcggaga ttcgtcagcg agcggcaatt aacgaacgcg
     7621 tgctggtcac cacactgacc aagcgaatgg cggaagatct taccgaatat ctcgaagaac
     7681 acggtgagcg cgtgcgttat cttcactcag atatcgacac cgtcgagcgt atggagatta
     7741 tccgcgactt gcgtctgggt gagttcgacg tattggtagg gatcaactta ctgcgtgaag
     7801 gtctggatat gccggaagtg tcgctggtgg cgatcctcga cgctgacaaa gaaggcttcc
     7861 tgcgttccga acgttcgttg atccagacca ttggtcgtgc ggcacgtaac gttaacggta
     7921 aagcgattct ctacggcgat aggagcactc catcaatggc gaaagcgatt ggcgaaaccg
     7981 aacgtcgccg tgagaaacag cagaagtaca acgaggaaca cggcattacg ccgcaaggct
     8041 tgaacaagaa agtggtcgat atcctggcgc tggggcagaa cattgccaaa accaaagcga
     8101 agggcagagg aaaatcgcgc ccgattgttg agccggataa tgtgccgatg gatatgttgc
     8161 cgaaagcgtt gcagcagaaa atccatgaac tggaagggct gatgatgcaa cacgcgcaga
     8221 atctggagtt cgaagaagcg gcacaaattc gtgaccagtt gcatcagttg cgtgagttgt
     8281 ttattgccgc atcgtaacag gatagcgaag aagactgatg acaaacggaa aacagtctga
     8341 tgcgctacgc ttatcaggcc tacattttct ccgcaatata ttgaatttgc gcggtttgta
     8401 ggccggataa ggcgttcacg ccgcatccgg cataaacaac gcgcactttg tcttcaatag
     8461 gaagccggaa ttttccttcg gatttccgtt aacctaaagc ctgtaacgcc ttttccagcg
     8521 cgttatgtaa caactggcgg tcatgacgat acggaatatc gctggcctcc agtacctcct
     8581 ggatcacaat ccgctctttc accgccgaga catccacttt tggcccgacg atgaccgcat
     8641 caatgacctt tttaccaaca tactgctcca taattgccag cttgctttcc agcttcaaat
     8701 tagccgcagg taaactcaac tcacgcccca gattgccgat ataaaccatc ggcgctggcg
     8761 tgcggcgtaa tgcctgggcg atttccttca gcagcagaat tggcatcagg ctggtataaa
     8821 aactgccagg cccaataatg atgagatccg cttcattgat agcgtgaacc gcctcacgcg
     8881 ttgcgggtac attaggcgtt aacaataact cttgaatcgg cgtagttaac tggtcgatat
     8941 tgacctcgcc gtaaacttca tgcccctgat cgtcaatcgc catcagatca acaggatgct
     9001 ctgacattgg aatcaaatgc gtatccactt tcagcagatt acgaattaaa ttgatggctt
     9061 ccagaggccg cacgctaagg tgatccagcg cctttaacat caagtttccg agattatgac
     9121 cggaaagttc gccattgccg ccaaaacggt attcaaacat cgcggaggcg acgttcggtt
     9181 ccgttatcag ctggttgagg cagttgcgca tatcgcccca agcaatgccg ccttctgaac
     9241 ggcgaatacg ccccgtcgag ccaccattat ccgtggtggt gacgataccc gttaaacgag
     9301 aacccaaaga cgaaagtgat gagagaacgc gtcccagtcc atgccctccg ccgagagcaa
     9361 cgacacgatc aagatcagcc agcgtacgat tgcgcatata gtttcctgga gtcagattat
     9421 ccgcgctaca gtagcgcaaa ttgcggggaa acagcaatta acctgccaat attgaggata
     9481 caaactattt tcttctatct ctaaaggacg atgcacgcta tgcctccctg atgatgtata
     9541 tcaaagtaaa accgccattt tcccttattc tgtagcgaaa tagcacgatc atgacgctat
     9601 atacatgatt acatagcgaa agtgtggatg gtaaaaatct catttacacg ctagtatcgg
     9661 cttaaccact aaacactcta gcctctgcac ctgggtcaac tgatacggtg ctttggccgt
     9721 gacaatgctc gtaaagattg ccaccagggc gaaggaagaa atgacttcgc ctcccgtatc
     9781 tggaaaggtg tacatggctt cacaactgac tgatgcattt gcgcgtaagt tttactactt
     9841 gcgcctgtcg attaccgatg tgtgtaactt tcgttgcacc tactgcctgc cggatggcta
     9901 caaaccgagc ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc gccgggttac
     9961 gcgcgccttc gccagtctgg gcaccgaaaa agtgcgcctg acaggaggag agccgtcttt
    10021 acgccgcgac tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta tccgccagat
    10081 tgcggtcacc accaatggtt accgtctgga acgcgatgtg gcgaactggc gcgatgcggg
    10141 acttactggc attaacgtca gtgtcgacag tttggacgcc cgccagtttc acgctattac
    10201 cgggcaggat aaattcaacc aggtcatggc agggattgat gctgcatttg aggccggttt
    10261 tgagaaggtc aaagtcaata ccgtgctgat gcgtgatgtt aatcatcacc agctcgacac
    10321 ctttctgaac tggatccagc atcgccctat ccagctgcgt ttcatcgaac tgatggaaac
    10381 gggcgagggc agcgagctct tccgtaagca tcacatctct ggtcaggttc tgcgtgacga
    10441 gctactgcgt cgcggctgga tccaccaatt acgtcaacgc agcgacggtc ccgcgcaagt
    10501 cttttgccat ccagattacg ccggagagat tggccttatc atgccgtatg aaaaagactt
    10561 ctgcgccact tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc tctgcctgtt
    10621 tggtgaaggc ggcgttaacc tgcgcgatct gctggaaggc gatacccagc aacaggcgct
    10681 ggaagcgcgt atttcagcgg cgctgcggga gaagaaacag acccatttcc tgcatcaaaa
    10741 caacaccggt attacgcaaa acttatcgta cattggcggc taaaacgtca aaaggagaga
    10801 tcagatgagt caggtaagca ctgaatttat cccgacccgt attgctattc ttacggtttc
    10861 taatcgtcgc ggtgaagaag acgatacctc cggtcactat ctgcgcgatt cggcgcaaga
    10921 agcgggccat cacgttgtcg atgaagccat tgtgaaagaa aaccgctacg ctattcgcgc
    10981 tcaggtatct gcgtggatcg ccagcgacga tgtacaagtg gtattgatta cgggtggtac
    11041 tggcctgacg gaaggtgatc aggctcccga agcgctgctg ccgttgttcg accgtgaggt
    11101 tgaaggtttt ggtgaagtgt tccgtatgtt gtcgtttgaa gagattggca cttccacgtt
    11161 gcaatctcgt gcggtagcgg gcgtcgctaa caaaacgctg attttcgcca tgccgggttc
    11221 gaccaaagcg tgccgtaccg catgggaaaa tatcatcgcg ccgcagctgg atgcccgtac
    11281 gcgtccgtgt aatttccatc cacatttgaa gaaataagta tgtcgcaact gacccatatc
    11341 aacgccgctg gcgaagcgca catggtggat gtctccgcca aagcggaaac cgtgcgtgaa
    11401 gcgcgggcgg aagcctttgt caccatgcgc agcgagacgc tggcgatgat tattgatggt
    11461 cgccaccaca aaggcgacgt atttgccact gcgcgtattg ccggtattca ggcggcaaaa
    11521 cgcacttggg atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt tgaagtcaat
    11581 ttacaggccg agccggagca caatcgggtg cgtatagaaa ccttatgccg cctgaccggg
    11641 aaaaccggtg tcgaaatgga agcgttaacc gcggcctccg tggcggcgct gaccatttat
    11701 gacatgtgca aagcggtgca aaaagatatg gtgattggtc cggtacgttt gctggcgaag
    11761 agcggcggca agtcgggtga ctttaaggtg gaagcggatg attaaagttc tttttttcgc
    11821 ccaggtgcgc gagttggtgg gaacagatgc aaccgaagtg gctgcggatt acccaaccgt
    11881 tgaagcgtta cgccagcacc tggctgcgca gagcgatcgc tgggcgctgg cgctggaaga
    11941 tggcaaattg ctggctgccg tcaaccagac gctggtgagt tttgaccatc cgctgactga
    12001 cggcgacgaa gtagctttct tcccgccggt aaccggaggt taagatggca gaaaccaaaa
    12061 ttgttgtcgg cccgcagccg ttcagcgtag gagaagagta cccgtggctg gcggagcgtg
    12121 acgaagacgg tgcggtagtc accttcaccg gaaaggtgcg caaccataac ctgggcgaca
    12181 gcgtcaaagc attaaccctc gaacactatc cggggatgac tgaaaaagca ctggcagaaa
    12241 ttgttgatga agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt caccgcatcg
    12301 gggaattatg gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg catcgcagca
    12361 gtgcgtttga agccgggcag tttattatgg attatctcaa aacccgcgca ccgttctgga
    12421 agcgcgaagc cacgccggaa ggcgaccgct gggttgaagc ccgggagagc gatctgcagg
    12481 cggcaaaacg ctggtagttt tttgttagcc ggataaggca ccgctgcgtc cggcaatttc
    12541 accgctcagt acatgcgttt acgctgcgtc gatgcacagc ctcatcattt tgcagtatcc
    12601 ttaagatatt ccttatatct tcaggagatc gtcatggaca gattcccacg ttctgattca
    12661 atcgtacaac cccgggctgg cttgcaaact tatatggcgc aagtctatgg ctggatgacc
    12721 gttggcttgt tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc ggccgtgatg
    12781 gagctgttgt tcactaaccg tgtcttttta atcggtctga tcatcgcgca attagcattg
    12841 gttattgtgt tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac gatgctcttt
    12901 atgctttatt cggcgctgac gggtcttacg ctttccagta tattcattgt ctataccgct
    12961 gcttctatcg ccagtacttt cgtcgttact gccgggatgt tcggcgcaat gagcctgtac
    13021 ggttacacca cgaagcgcga tttaagtggc tttggcaata tgctgtttat ggcgttaatc
    13081 ggcattgtgc tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt gatgtgggca
    13141 gttacctaca tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac tcagaaattg
    13201 aaaaatatgg gtgagcagat tgatacccgc gacacgtcga acctgcgcaa atattccatt
    13261 cttggtgcgt taaccttgta tctggacttc atcaacctgt tcctgatgtt gttacggatc
    13321 ttcggcaacc gccgttaatt ctttttcacc atcactatcc cggccatgtg tcgggatttt
    13381 tttcttacta aaaacgacac ttaacaagta ccaggtataa aatatgcctt atgagcgagc
    13441 ggaatcgttt tccgtatcca taaatacaaa agtattttgt atgcgtggtt ttaaatttat
    13501 tgatggtgaa tattaatgtt ttttctaagg acaactaaaa gaatggaatc atactcgcaa
    13561 aacagcaata aattagattt tcagcacgag gccaggatat taaacggtat atggctcatt
    13621 accgctttag gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt
    13681 acggcaacca aatatgattc accaccaatg tatgtcgcca tagggttatt attactttgt
    13741 atgtatggct taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat
    13801 ctgtttttac tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt
    13861 atcatcgtgt tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta
    13921 tttaatgttg atcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc
    13981 ctggtaatca tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc
    14041 ttaatgattg tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaactg
    14101 gcaggaaaat gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca
    14161 ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg
    14221 cttgtctggc aaaggcatac gcgttttttt cattagtcgt aaatgcagat actaaagaac
    14281 ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc ttggctttcc
    14341 agtagcaggt aacagatcag cgccagcagc agcgggataa agtaatacag gacgcgataa
    14401 gcgagcaggg cggcgataat gctccctttc gaggtatgct ccccagccag tagtgcgata
    14461 aacaccgctt ccagcacacc gatccccgcc ggaatatgca cgatgacgcc agcaatacta
    14521 ctaaccagta acacgcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag
    14581 ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat
    14641 ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa
    14701 gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc
    14761 gtagtttgat cgacatacca gtaatccggc aactccacca cgcctgcggt aaagataatc
    14821 cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagga aatccgcgta
    14881 atagtgctgc ccggtaaccc tagtcgggag tacaaacgat agcgcatacc aatgccgccg
    14941 acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg
    15001 cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc
    15061 agatagctga cgaccaccag cccgaccgca ctaagcagcg caacgcgatt gtagtcgcgg
    15121 atgaccttcc agacctcttc ccagtccact tttttggcgt agaccaccag caacacaatc
    15181 accgcgataa aaaacagcca ggtgaggatc ttctttgcta agcgccagcg cgggtgtgat
    15241 ttactcatca ggggtttacc cccgtgtttt cagtttctac ccgatcctgc gtttccattg
    15301 tcggttgtgc ggggggatca acctgcgtca gacgtggtgt gtgtgccgga agccagccaa
    15361 ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac gcttttggtc aggttccacc
    15421 aggtgcgttt gggcagcatg gtttcatcca cctgctgaca atctgcggca ataatgccgt
    15481 tcagattatc gcgcagcgtc tggttaaaat gacgatcgtg gatgatgaca tttgcttcga
    15541 gattcagtga caaactgagc ggatcgagat tactggaccc tactgtcgcc cagtgatcgt
    15601 ccatcaatgc cactttgccg tggagcgggc ggcggcggta ctcaaaaacc tgaacgccgc
    15661 ctttaaccag atagttatac agcaagcgcg caccgactct gacaatcggc atatccggtt
    15721 cgccctgaat gatcagtttg atccgcaccc cgcgccgtgc cgctttacgc aaggcgtgta
    15781 aaaatcgata gccggggaag aagtaggcgt tggcgataat cacttcccgc tgcgcctgag
    15841 tgagcatttt cagataatgg cgttcaatat catcgcgatg ttcttcgtta tcgcgccaga
    15901 ccagcaatac ctgcgcttct cccggctggc ggttctcttc cgctttgtga tgacgtcgcc
    15961 accagcgtcg tgccgcgctc tgtccaggca ggttttccag ctcaaactgg agaatatctt
    16021 cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt agcctctgga ccgtagctgg
    16081 acatatgctc ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg tcgatcacca
    16141 caattttgcg atgcatccgg cgaaacacat tggtgcgcat accaaaaagg cgagggcggg
    16201 gatcgtagta gcggaacact acgccagctg ccgtcagttc attgacaaac tcatcgctga
    16261 gatccggcga accgtagcca tccagcaaga cttccgcttt aaccccgcgt tgcgctgctg
    16321 ccagtagtgc cgcatgcagc tgtttgccga cgttatcctc aaaccagata aacgtttcaa
    16381 gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa tattgctcgc
    16441 cgttttccag caactggatc ttattgcctt cgcgccagct acatttcata aatgaatctc
    16501 cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc cgcagcggca acgcggttgg
    16561 cgcgctggcg ctggcatttt tgacgtagat cctgtccagt cgtagtagag gaaattgcac
    16621 cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa atctcatcca gtccggcctg
    16681 cacttttaac ggatgattag ctttttgccg ccagtcattg aaatcacccg ccaccaatac
    16741 cggttcgccg tccggtagct cattcaccca ttcggcgagc atcgcaagct gcgcctgacg
    16801 gtgcgcctca cgcaggccca gatgtacgca catcacatga atcgcttttc cggtcatcgg
    16861 cggcacaatg cggcagtaga gcacgccgcg cttttccgca ccatcgaccg aaacatcgcg
    16921 attctcataa tgttcaatgg gataacgcga cagtacggcg ttgccgtgat gcccttccgg
    16981 gtatacggca ttgcgaccgt aggcaaaatc gctccacata gtgtcggcga gaaactcgta
    17041 gtgcgaggta tcgggccagt tttccacatg cagcggatga acttcgtgcg cgcccatcac
    17101 ttcctgcagg caaacaatat cggcgctgac ggtacgcacg gcatcgcgaa gttccggcaa
    17161 aatgaagcgt cggttaaacg cggtaaagcc tttgtgaatg ttgatggtga gcaccttgaa
    17221 cgaaaattgt tgtgtttgat cgggcataat tttcctgtct ttgcctctta tctcattgaa
    17281 atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga attttccgta aagttcggta
    17341 ctctgagtaa gtagagataa attcttcagg agagaagcca tgaagtggca acaacgtgtt
    17401 cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc atttactggt agtggcgctg
    17461 ctggtggtgg gctggatgag taagactctg gttcacgtcg gcgtgggatt atgcgcactg
    17521 tattgtgtca cggtagtgat gatgctggtg tttcagcgcc accccgagca acgctggcgt
    17581 gaggtggcag acgtgctgga agagctgacc acgacctggt attttggcgc agcgctgatt
    17641 gtgctgtggc tgttgtcccg cgtactggaa aacaactttt tgctggcaat tgcagggctg
    17701 gcgatccttg ctggcccggc ggtagtgtct ttgctggcga aagataagaa gttacatcac
    17761 cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg gccgttatca ccagtagcgg
    17821 ccacaaacta ttccacacaa tatccagact cgcatccttc aaataaatct gcttggtaat
    17881 gtccgtaaag tggcgaatag ggttaatcca cgtcagattt tgcagccaca ctggcatgtt
    17941 ttccaccggc gaaacgtaac cggaaaggag aatggcgggc atcataaaaa caaacacgcc
    18001 gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc aacagaccga atcccaccag
    18061 cgataaaccg taaatcacca tcgtaaagta gaacagcgct agcgatccgg cgaaggggat
    18121 ttgatacgcc cagataccaa tcgccagcac aatggtggcc tggaaggtgg cgacaattaa
    18181 cgccggtacg gctttgccga tgaagatctg ccaggtggtg agcggcgaaa ccagtagctg
    18241 atcgagcgta ccttgttcac gttcgcgggc gacggaaagt gaagtgacga tcattacgcc
    18301 gatagtggtg atcatggcga tcagcgacgg caccacaaac catttgtagt cgagattcgg
    18361 gttataccag ttgcgtacca ccagctcgct gttgttgggt ttcggttttc cttccagcag
    18421 ctcctgctga taatttttga cgatctgttg caggtagttg gcggcaattt gcgcactgtt
    18481 ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc tggaaggtat ccagtttgcg
    18541 cgagaagtca gccgggaaac gcaccagtag taacgccttt tgtgtgtcga tggtcgggcg
    18601 gatctcctgt gggcttttca gcagcagcac atgtgtaaag gcgctggcgc gggcaaaacg
    18661 ttgagtaagt tccaccgaat gctcgccgtt atcttcatcg tagatggcga tggtggcgtt
    18721 agtcacttcc agcgtggcgg cgaacgggaa caggatcacc tgaattagca cgggtaaaat
    18781 cagaatcgcg cgggtttgtg gttcgcgcag caacgactgc aactctttgc ggattaacgt
    18841 ccataagcga tgaaacatgc tcttctccct aatccagccg acgtttggtt ttcagccacg
    18901 tcaggccgat aaacatcacc gccgaagcga tcaaaaacag cacgtttacc accagcacca
    18961 ctggaatatt cccggcgagg aacaggcttt gcagggtgct gacgaaataa cgagcgggaa
    19021 taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa ataaagccgg
    19081 aaagcataat cgacggcaga aaagcggcgt tcagggcgac ctgagcggca ttgaactggt
    19141 tgcgggtaat cgtggaaatc agcagcccca ttcccagggt actgagtaaa aacaggctgg
    19201 agataaaaaa cagaatcagc agcgaaccgc gatacggcac gccgagaata aacactgaca
    19261 ccagcataca cagcaacatc gccagcatcc cgagaaagta ataagggatc agcttacaca
    19321 gcagcagttc cgtgcgggta atctccgtag agagcagagc ctccatggtg ccgcgttccc
    19381 attctcgcgc caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg ataatggtca
    19441 ccgcacccgg gataataaag tgctggctta tcgccgccgg gttaaaccag tagcgggttt
    19501 gtacatcaat aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc atttgccaga
    19561 tctgccagat cccttcgaca tacccctgca caaagttagc ggtattcggc tcactgccgt
    19621 cggtgatcac ctgaatcggt gcggtggcgt tggcgcgctc catctgttcg gcgaaatcca
    19681 cgggaataac caccagaccg cgaattttcc ccgactgcat tttggcgatc agttcctgac
    19741 ggttatcgct gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg gtgaaatcca
    19801 gcgccgcttc gctacgctgt tccagcaaaa tcccgacccg cagcttgctg gagtcgaggt
    19861 taatgccgta accaaaaata aacagcagta gcagtgggat cactaccgca atcagccagc
    19921 tactcggatc gcgaacgatc tgccgcgtct ctttgacgca cagcgcccgc acgcgatgcc
    19981 aggaaaggat cgggttactc attgctatgc tccttatccc agtcgtggat caactgaata
    20041 aaggcttgct ccatggtggg atcgggttgc tcatcgttag ctgactgtgc tttcaaatcg
    20101 tccggcgtac cgctggcgat taatttccca cggtacacca ggccgatgcg gtcgcaatat
    20161 tccgcttcat ccataaagtg ggtggtgacc atcaccgtga cgcctttctc taccatgctg
    20221 ttgatgtgca gccaaaattc acggcgggtg agggggtcaa cgccggaagt cggttcgtcg
    20281 agaaagagaa tatccggttc atgcatcagc gaacaggcca gcgccagccg ctgtttaaaa
    20341 cctaatggca gttcatcggt ggcgtgtgag gcaatacttt tcaggccaaa tgcctcgctc
    20401 atacgggaga ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc agagaaaaag
    20461 cgtaaattct gttcgaccgt caggttgccg tagagcgaaa atttttgcgc catatagccg
    20521 agatgctggc gcgctttacc ggaactctct ttcagatcca tccccagcac cagcgcctgg
    20581 ccggaagtcg gcaccagcaa accgcacatc atcttaaagg tggtcgattt acccgcgccg
    20641 tttggcccca gcaaaccaaa aatctcccca cgtttaacgg caaagttgac gtgatcggtg
    20701 gcggcaaaat caccaaattt cttggtcagt tctttcgctt cgatcaccgt ctcgccgggt
    20761 gtgccttcta ccgtatgcaa tattgcaccc agcggcgatt ctgaggttcc ggcaccgccc
    20821 agcaaatcaa taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt gatttccggc
    20881 atcccgtcgg catggcgaat atcgtctggt gtggcctctt ttttgaggat cagacgtacc
    20941 gatttcccct gaatcatgcc atcgctgacc tgcggcagtt tcaaggcgcg ttgcaacagt
    21001 ttgcggttgc cctcgtgtgg actggtcatc agaaagctgc gtccggccat ggtttgtgtc
    21061 agggcttttg gttctccctg atacagcaac tcgccttcgt tcatcagtaa cacgtcacgg
    21121 cactgctcgg cttcgtcgag atacgaggta ctccagagga ttaacatccc ttcgcccgcc
    21181 agctcatgca ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc gacgccgggt
    21241 tcatcgagca gcaacacttt cggttcgccc accagggtac aggccaggcc gagtttttgt
    21301 ttcatcccac cggagagctt gcccgccagg cgtccggtaa acggcccaag agacgtaaac
    21361 tccagcaggc gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg caaatccgcg
    21421 tacagattga ggttctccat caccgtgaga tcttcataca gaccaaattt ctgcggcata
    21481 tagccgagca cggcgtgcag cgcgccgtcg tttttgatcg gatcaaagcc aatcaccgtg
    21541 gcactgccgc tgtcgggttt cagtaatccc gccaacatcc gcatcagtgt ggttttacct
    21601 gcaccgtccg gccccaccaa ccccgtcaca taaccggcgt gaatggtaca atcgagcggc
    21661 gcgacggcgg gcttgtccat gcccggaaag cgtttttcca ggccgttcag cgtgataacg
    21721 gcatcattca tgtcctgcct cgtcaccgaa ttgtaccgtc actggcattc cctggcgtaa
    21781 cgcatcatcg gcgtcggtca ccacaatacg caggcgatag acgaggtcgg tacgcagatc
    21841 cggtgtttcg acggttttcg gggtaaattc agcagtcggc gaaacgaaac caatctgccc
    21901 gtgatacggc ttgtccgggc gaccatcggt ataaagcagc actttgcgcc ccggctgggc
    21961 ctggtcaaga ttccgttcat caacataagc gcgcacccac accggacgcg ttagtgaaac
    22021 ggtaaacacc gtgccacctt cattgagaac cgtgcctggc tctaccgcgc gcgttaacag
    22081 cgtgccatca gacggtgcta tcaacgttga gtcctgtaaa ttcagctccg cctgcgccag
    22141 ttgcgcctgc gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt cacggttacc
    22201 ggaacggtac tgacgcaatt tatcctgtgc tgatttcagc gttgcctgtg cctggtcgcg
    22261 cgaggagcgg gcattttcca ggtcatttgc cgaaatggtg cggcttttcc acagtccttg
    22321 ctggcggtta tagaagttct gcgcatagtc ataggcggct tgcgcctgtt tcaccgctgc
    22381 ggcggcctga gcgatttctt catcgcgata cccggcaagc atcagatcat actgcgcctg
    22441 tgccaccgag acgcccgctt tcgcctgcat cagggcaatc tcatacggct tgtgatccag
    22501 ttcgcccagc acctggcccg ctttgatagc atcaccttcg tccaccgcca gcgattcaac
    22561 gcgcccccca acacggaaac taagattcac cgtacgaata tccacgttgc catacagcgt
    22621 caggccgtta tcctggcggc tttgatacca ccagtagcct ccggcaacca cggcggcaag
    22681 taccactacc gccagtccga tcacgacagg ttttttcatc actacaaact cctttgcgat
    22741 aatccttgca aaatcaggtc gatatgacag gtcaccgtct ggttgatcag ttcggtcttt
    22801 tcttcatcga acgcggtcca gccggtacgt aacagaattg tttctttgcc aagacggaac
    22861 gccagaatct caccaatcaa cgcatgggta tgaaggatca tgcgggtgtc attagcgtcg
    22921 cagccggtcc aggcggcaat caggcgtgtc aggtggctgt gtagcggact aatcacctgt
    22981 tcgtgcacca ggtggtaggc tgccgtggga gagagctgct cacgggagat aaacttgctg
    23041 aggttgacgg tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc gcgaaggatc
    23101 agttcacgga tggcagcccg atcaggctgt ggttgggcga acaagcgttc ggcttcctcg
    23161 gcatgcggac ggaactgctc gccaataaaa tcggcaatcc actgggcgca ggcgaggtac
    23221 aaatcttctt tcgaaccgaa gtagtaggtg atggcagcga tattctgccc ggcctgggcg
    23281 gctatctcgc gggtggtggc gttcattcca tattcaccaa actgcgccag tgcggcagca
    23341 atcagctgtt ttttcgcctg ttcacccttg attgtcatgg caggattatt catcgcacag
    23401 tccattctta atcaaatgat tgattaagat tatgactcca tatgggagtt gtccagtatg
    23461 gctaagaatt ttagctacgc cagtcacagg gataatttat gcgctgcgtc acaaaaactg
    23521 ctacactccg ctccctcatg acattgtggt ttttgtcatt ttccttttca gtatctccct
    23581 gaaaactaca ccggtaacgg tcggggcggt tcggagtagt tatgtctttc gattctttgg
    23641 gtttaagccc tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa cccaccccta
    23701 ttcagcagca ggcgatccct gcggtgctgg aaggccgcga cctgatggct agcgcccaga
    23761 ccggcaccgg caaaacagcg ggctttacgc tgccgctgtt gcaacacctg atcactcgcc
    23821 agccgcacgc caaagggcgt cgtccggtac gtgcgctcat tcttaccccg acccgtgaac
    23881 tggcggcaca gattggcgaa aacgtccgtg attacagcaa atacctgaac attcgttcgc
    23941 tggtggtgtt tggcggtgtc agcattaacc cacaaatgat gaagttgcgc ggcggcgttg
    24001 atgtgctggt ggcaacgccg ggacgtttgc tggacctgga acatcaaaat gcagtgaagc
    24061 tggatcaggt tgaaatcctc gtcctcgatg aagctgaccg catgctcgac atgggcttta
    24121 tccacgatat ccgtcgcgtg ttaacaaaac tgccagcgaa acgccagaac ctgctgttct
    24181 ctgcgacctt ctctgacgat attaaagccc tggcggaaaa actgttgcac aacccgctgg
    24241 aaatcgaagt ggcacgccgc aataccgcgt ctgatcaggt gacccagcac gttcactttg
    24301 tcgataagaa acgcaaacgc gaattgctgt cgcacatgat tggtaaaggg aactggcagc
    24361 aggtgctggt gtttacccgt accaaacacg gcgctaacca tctggctgag cagctcaata
    24421 aagatggcat ccgtagtgtg gcgatccacg ggaataaatc gcaaggtgcg cgtacccgtg
    24481 cgctggctga ttttaaatcg ggtgacattc gcgtactggt ggcgaccgat atcgctgcgc
    24541 gcggtctgga tatcgaagag ttgccgcacg tcgtcaacta tgaactgcca aacgtaccgg
    24601 aagattatgt ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt gaagcgttgt
    24661 cgctggtgtg tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg ctggaaaaag
    24721 agatcccgcg cattgcgatt ccgggctatg agccggaccc gtcaatcaaa gccgaaccga
    24781 tccagaacgg tcgccagcaa cgtggcggca tcaggctgat gaacaaacgc aaaactgcct
    24841 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    24901 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    24961 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    25021 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    25081 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    25141 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg
    25201 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    25261 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    25321 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gtattcccgt
    25381 caaccttgat gggcctggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    25441 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    25501 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    25561 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    25621 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    25681 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    25741 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    25801 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    25861 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    25921 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtcca
    25981 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    26041 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    26101 gatctcgaca cttataaatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    26161 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    26221 tatcgtgaat gcccgttttt tgttactcgt cgggagattc aggaagcgga agtggtggtg
    26281 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    26341 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    26401 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    26461 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    26521 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    26581 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    26641 cctgatgaat tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    26701 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    26761 ctgcatcggt tgattttaca gatgaaccgc gcgctgggga tgtttgaggt gcaaagcaaa
    26821 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    26881 cgggaagagc gcgaagggca gctacacctc tggtttcact gcgtgggaat acgtgtcagc
    26941 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    27001 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    27061 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    27121 attccccgga tgcgctttga gccttccatc gacaacgaag agcaacacat tgccgaaatg
    27181 gcggcctttt tccgtgaaca ggtggagagc aaaaaatatc tcggtatgtt ggtactgttt
    27241 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgttg
    27301 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    27361 aacggtgagc gtagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    27421 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    27481 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    27541 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    27601 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgacaaaaaa ttatggcaag
    27661 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    27721 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    27781 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    27841 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    27901 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    27961 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    28021 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggct
    28081 aacctgacgc cattattggc gattctgctg cataaactcg gttttcctgt ggtggttcac
    28141 ggagttagcg aagatccaac tcgcgtgctg acagaaacca ttttcgaact gatgggtatt
    28201 acaccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    28261 atgcctgtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    28321 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag
    28381 gcactgtgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    28441 agcgatatcg gcgggtgggc gttactaatg catggtactg aaggcgaagt gtatgctaat
    28501 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    28561 aaacaggacc ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    28621 cagtggactg agcgttgcct ggctggcagc gaagcgattc ccgaatcgct gaaaatccag
    28681 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgatgg cctggcgcgc
    28741 gttaatcagg cattttaatt tttatctcag ccggatgagc cacgtcatcc ggcttttacc
    28801 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cgcgcccggc gtgcagcata
    28861 aaaaaacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    28921 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    28981 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc
    29041 atggtattgg catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta
    29101 accatcatgc caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg
    29161 cattcggtca ggtcgcggca cacgaagcga tggcgctggg gattgagaaa gcgcatcagc
    29221 acggtattgc tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    29281 cggagcagtg ttcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    29341 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccc ttctgtgtgg
    29401 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    29461 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    29521 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttggggtcgc
    29581 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    29641 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt gcaaaccagc cccgatgcca
    29701 ttcttaactg catgaccact atcatcatca acccggaact gttcggcgcg ccggattgta
    29761 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gacgataagc
    29821 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga
    29881 ttccattgga tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt
    29941 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    30001 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    30061 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    30121 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    30181 atgcggcata aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    30241 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgccgct
    30301 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    30361 ccgattttat tcatgttctg tgcctgggat gccgttaccg gttccgccgc gaacacgcca
    30421 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    30481 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    30541 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    30601 tctaacatac tgataaacca tttatttgtt gtacttatgg acttgtttta tgccgcgtgg
    30661 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctatg
    30721 catctcgcca gttgtagtta acgtaactgt ctgtctttcg aacctctgcg attatatcct
    30781 gtatacgctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    30841 ggaatggctt cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatacagg
    30901 ctttcgccac gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    30961 atctgttcgt tgacggcgtc atcgttagcc caagcggatg ccatatcgtc ctccccatat
    31021 caatgcttgt acagttaagt gtagctaata tagggacgaa cctgggcagt tcaagcgtca
    31081 gatctccgac cattcccgca gcagattatg ataaagattg agcagcgaca ggatctcttc
    31141 attttcacca tagcggcttt tcagcgactg aatatttttg tccagttcaa acagcatggc
    31201 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    31261 gcgggttacg ggtgtcacgc aatgcaggct gctggaggga tacaacacca gatcacctgc
    31321 cgggagtttt acccgatgtt gtccaaaggt gtcattaacg accagttcgc cgccgtcgta
    31381 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtgcgcatcc agccgttttg
    31441 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    31501 attaaacagc ggcgtggaaa gggtacgcgg caaggcagcg gcaaagaaca atgcatgttg
    31561 gttaaccgcg ttcagcacct cattttgcag tgcggcgtat aacgcgctgc gggtgtctac
    31621 ttgctgattg ttcttaactt gtgcaccttg tgctccggtg gtgacgcgtc catccaccca
    31681 ttcggcttgt tccagttgtt cgcgaaaatg ggcgacgtcc tgtggcgata acacgccggg
    31741 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    31801 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    31861 ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag
    31921 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttctgta
    31981 aatgctggcg ttcccaccgc gccgtctgaa cctttatgta tactgccgat atagcgtgcg
    32041 cccgcgccaa cagagatatc atcggttgcc tgatattggc tccataaggt gaaggcgtgc
    32101 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    32161 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attccctgcc
    32221 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca
    32281 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacaggcgt
    32341 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    32401 tcggtgcggt tggcactgtt accactgcca gactgcgcaa gggcgaagtt gttgccgccc
    32461 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtggtac
    32521 agcgccccgg ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgact
    32581 ggagaacctt ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag
    32641 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    32701 aaatcacggg tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca
    32761 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa ttctgctgtc aggatgataa
    32821 atatttaccg ccggtaacgt caccggatta acgccatagt tagtctgcgt ttcacgggta
    32881 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    32941 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgactag
    33001 gtccagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    33061 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    33121 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    33181 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgct
    33241 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    33301 ttgtgctgag tgacatgcag ataattaaga tacaaacgat ttgctgtacc aaggccaaaa
    33361 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgg
    33421 gttttttcgc ccatcacatt caggcgcacc gcggtagtat caccaatgac ctgattgacg
    33481 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    33541 tcattgcgcg gctgcttgct gatcatattg atcgagcctg tcggtgcgct gcgcccgtag
    33601 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    33661 acgctgccga tatcgcgaat accatcaata taaatactgt tagaggtatc ggcaccacgc
    33721 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    33781 c
//
LOCUS       DAECOD010000037        33542 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.037, whole genome shotgun sequence.
ACCESSION   DAECOD010000037 DAECOD010000000
VERSION     DAECOD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.037"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="KKO15_001789"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="KKO15_001789"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBD6360601.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="KKO15_001790"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="KKO15_001790"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBD6360602.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KKO15_001791"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KKO15_001791"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBD6360603.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KKO15_001792"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KKO15_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBD6360604.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KKO15_001793"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KKO15_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBD6360605.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KKO15_001794"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KKO15_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBD6360606.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="KKO15_001795"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="KKO15_001795"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBD6360607.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="KKO15_001796"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="KKO15_001796"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBD6360608.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="KKO15_001797"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="KKO15_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBD6360609.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KKO15_001798"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KKO15_001798"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBD6360610.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KKO15_001799"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KKO15_001799"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBD6360611.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="KKO15_001800"
     CDS             8212..9075
                     /locus_tag="KKO15_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBD6360612.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="KKO15_001801"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="KKO15_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBD6360613.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSITQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="KKO15_001802"
     CDS             10411..11028
                     /locus_tag="KKO15_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBD6360614.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KKO15_001803"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KKO15_001803"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBD6360615.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="KKO15_001804"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="KKO15_001804"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBD6360616.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="KKO15_001805"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="KKO15_001805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBD6360617.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="KKO15_001806"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="KKO15_001806"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBD6360618.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="KKO15_001807"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="KKO15_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBD6360619.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="KKO15_001808"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="KKO15_001808"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBD6360620.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KKO15_001809"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KKO15_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBD6360621.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="KKO15_001810"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="KKO15_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBD6360622.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="KKO15_001811"
     CDS             21859..23568
                     /locus_tag="KKO15_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD6360623.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KKO15_001812"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KKO15_001812"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD6360624.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="KKO15_001813"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="KKO15_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="KKO15_001814"
     tRNA            27625..27719
                     /locus_tag="KKO15_001814"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28019..29203
                     /locus_tag="KKO15_001815"
     CDS             28019..29203
                     /locus_tag="KKO15_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6360625.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29615..30658
                     /locus_tag="KKO15_001816"
     CDS             29615..30658
                     /locus_tag="KKO15_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD6360626.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31173..31601
                     /locus_tag="KKO15_001817"
                     /pseudo
     CDS             31173..31601
                     /locus_tag="KKO15_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31631..32782)
                     /locus_tag="KKO15_001818"
                     /pseudo
     CDS             complement(31631..32782)
                     /locus_tag="KKO15_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32847..33501
                     /locus_tag="KKO15_001819"
                     /pseudo
     CDS             32847..33501
                     /locus_tag="KKO15_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8399 a         8819 c         8268 g         8056 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct ctggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcac
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattatc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacc gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc
    28081 tgatggcggc ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa
    28141 gtaccgcttt gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct
    28201 agccgatgct cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc
    28261 aggggaggca aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga
    28321 ggctattgct cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga
    28381 tgaaatcatt gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc
    28441 cgaaatcaaa ccaatgaagt tactggaagc actgcgtaaa atggaaaaac gtggagctct
    28501 ggagaaaatg cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac
    28561 tggccgggca gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa
    28621 agtacatttt ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg
    28681 ttataccgga agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg
    28741 aacacaagaa ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga
    28801 gatcccggca gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt
    28861 cattatgcta tttaagcagc ttgaaccaat ctcaaaacat catcccctag tctttatcgg
    28921 tagaaatgat ccccgcaaac caattagtaa ggaaagcatt aaccaagtaa ttgaattact
    28981 tggatataag ggaagactca cagggcacgg tttcagacac accatgagta caattctgca
    29041 tgaacaaggc tttaattccg cctggattga aatgcagtta gctcatgtgg ataaaaattc
    29101 catcaggggt acctataatc atgccctgta tctcgatggc cgccgtgaaa tgatgcaatg
    29161 gtacgctgat tacattgatt cactctccag ccgggaaagt taagtcagaa atacttttcc
    29221 ctgatgtttt cctcaattaa gtattttatc ctctggccta caaggctgga ggatttttgt
    29281 tattatatta tttttttgcg tcttcaaact tattacaaaa acataattaa aaacaaattg
    29341 acatttttaa attggcaatt acaaaataca gccttgcagt tatttcgata cctttagcaa
    29401 aggaatagtt gattcagata taatttagca tttcaatcat ctgacatcat tataaaaaat
    29461 gagattcaca taaagataaa attattgttc tcaaggaaag catgaagggg cttcgtaagc
    29521 aatattcatt cggtgccaag acaggacttg atacacagaa tctcaacttt tatgctctca
    29581 gactatatca ccacatgttt tacaggctca gaaaatgaac gatagattat gctttgaagt
    29641 gcacgataac aagggttatt tcgtatttcc ggatacctgg tttggcccat tgctggggga
    29701 gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc agcgagacga gttacatcaa
    29761 caaactccga cgccttgctc aacgggagcc ggactttatt gatatacacg ctcatctggc
    29821 atatgcattt ctggaacaga atgccccgcg taaggcactg aacgccgctc ttaaaggact
    29881 ggcggcaggt aatcgtatca tcccggaaag tttctgtggt gaaataatct ggatgcatcc
    29941 cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca
    30001 gcgccatcag gatgcggtta tgctcactga taaaatactg gcatacaatc ctgaagataa
    30061 tcagggggct cgctggttgt tgggttcaga gctgttacgc accggcgatc atgagcgagc
    30121 cttcagtgta ctgaaagagc atgctgatga attttcacct tactggtatg aactcggtct
    30181 cctgcatttt ctgaacgggg aacatgttaa ggccgccaca gcttttcgcc atggctttgc
    30241 cacaaacact tacatcgcag aaatgctgtg cggaaatctg catccatttc cacttgctgt
    30301 gtggcatgac ttctccggct ctctggatac ggcagaagat tactatgcaa catactcacc
    30361 attatgggga caatatccgg aagcattgct gtttgtaaac tggctctata accattcctc
    30421 cgtgctgcat gaacgatcag aaatcattaa atgcgccgaa atgctgattc aggaagatga
    30481 tttcgaaata tgcgaaagta ttttaagaca gcaggagcac ctgtggaaaa gaattgacaa
    30541 gacactttct gaggaaattg tacagaaatg cagaaatatg aacggtgaat atatctggcc
    30601 ctggatattg cccttttccg cagcagggat gaaacattca agcatccagt atcagtagat
    30661 attgcattag tgtaacttta aaacaagtaa taatttatta tccaataata acactgtgtt
    30721 ccttccccag gtgattaccc tggggttatt ttctgaaagt ttcctttgca aaaggatagc
    30781 gttgtaaatt actttaacga attactccat gaactcatta aagttctttc gtaatacttt
    30841 atcgtcaaac acgttatagc tggcatcaaa aaccacagat ttaccctgcc agcgggtaat
    30901 cccctcctct ggcgttttcg gttgaactgg tatccccgtc caacacaggc agccattata
    30961 aacagtaaaa aattgtattc gctgttatga aatcaatagt cctgacgtac tgttgaatgt
    31021 gcttgtgttc tgaacctcgt acaaccagta catttgatat cgaattataa acaaaacatt
    31081 gcatccaact tttttaatat ggacaatagt tgtccatacg gtggttttat attcgataaa
    31141 tcaatggatt tcattgagtc acaggactgg taatggatga aaatgcttta aggtttgcct
    31201 catactggcg caactcgctt gcagatgctg agtcaggaaa gggctgtttt gaacggaaag
    31261 acgcccaaaa tttcactcac tggcatggga tagcggcggg acgtcttgac gaagcgattg
    31321 tcaataaatt ttttaaggga gaaaaagacg atgtcgaaac tgtcaatgtc atcttacgcc
    31381 caaaagttta tttccggtta ctgcagcatg gtaaggaccg ttccgcaggc acgcctgata
    31441 ttgttacacc gatagtgacg ccagccttgc taagccgtga gggtttttta tatccgacgc
    31501 cagcgacctc cattcccaga gacctgcttg aacctttgcc aaaaggagca ttttcgattg
    31561 gtgagattgg gcagtatgac aaatacaaga cgacccatac tgatcctacc cacgtaatgt
    31621 ggacacggcc ctaagcgaga ttatggtttt caaattgttc cgggctgaga ccgccacagg
    31681 cactgtgacg acgccagcga ttgtaatcac actcgatata attaaacacc gttgcccgca
    31741 ttatttcccg gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga
    31801 agctttccac acaggcattg tcataacagt agcctttcgc actcatactg ccacgcaggt
    31861 tgtgtcgctt cagcattgcc tgataatccc ctgaacagta ttgaccacca cggtccgtat
    31921 gaacaatgac gctttccggg cgtcttctcc gccacaacgc catttgcagt gcatcacagg
    31981 ccagttgtgc tgtcattcgc ggtgacatcg accagccaat gacggcgcgt gaccacaggt
    32041 cgatgactac tgcgagatac aaccagccct catcggtacg caagtacgtg atgtcacccg
    32101 cccacttctg gtttcgggcc actggcgctg aagtcctgct ccagcagatt ctccaatacg
    32161 ggcaggccat gtgcacggta gctgaccggg ctgaacttcc ggctggcttt cgcccgcagc
    32221 ccctgacgac gcaggctggc ggcaatggtt ttaatattga actccggcag ttcgtcagca
    32281 aggcggggag caccgtatcg ctgctttgcc tcaaagaatg ccttatggac agcggcatcg
    32341 caggtgagcc gaaactgttg gcgcaggctc atctggtgac gacgcctgag ccagacatac
    32401 cagccgctgc gggcaacccg aagtacacga cacatcgctt tgatgctgaa ctctgcccga
    32461 tgattttcga taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt
    32521 ggaggatgga cagttcctca gcctgctccg ccagttgtcg tttaaggcgg acattttcag
    32581 cggccagttc gctttcgcgc tctgacgaac tcatttgttg ctgctgttta ctgcgccagg
    32641 cataaagctg agattcatac aggctgagtt cacgggctgc ggcggccaca ccgatgcgtt
    32701 cagcgagttt cagggcttcg ttacgaaatt caggcgtatg ttgtttacgg ggcttcttgc
    32761 tgattgatac tggttttgtc atgagtcacc tctggttgag agtttactca cttagtcctg
    32821 tgtccactat tggtgggtaa gatcaccctc cattaaaact aaaacgggcg ctttctgata
    32881 tatcatctgt aagcgcagta ataccattag tagcacctct cggcggcgca ttgattattg
    32941 aatattataa ctggaatgtt atatttcttg ttatgatgct gttcagtatt ataataatgt
    33001 tattatcacc aaagataact catgcaaatg agaaagtaat atgtcaaaac agtgcatcct
    33061 ttgtgaccaa agagtttatc tgcggcacta tacttgtctc attaatactc agttctctat
    33121 tttgctacat atcaatatca ccatcaattt tcatgcatac ttatggcctg agtactttta
    33181 gttactcaat tattttctcc ttctcagttt tatttttcat aatcggaaat caattatcaa
    33241 agattgaaaa attatccaac ctatggtttt tattacccaa tagccaattt tatcgcatta
    33301 caaataaatg gttcaaaaac aggatttgcc gcatctatta caggttttac tcaaacaata
    33361 agcgctggta caatcagttt cctgtcagta accgtaagcg acaacggtgt tggtcccgga
    33421 actactttag gtcttggttc actaataact tcattactgt caatagccgt aatcgccatt
    33481 gcaggaaata ataaaaagta agtgtactct gactgcgtat gtatctactt gaaccgcccc
    33541 gg
//
LOCUS       DAECOD010000038        33166 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.038, whole genome shotgun sequence.
ACCESSION   DAECOD010000038 DAECOD010000000
VERSION     DAECOD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.038"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(47..1906)
                     /gene="gss"
                     /locus_tag="KKO15_001820"
     CDS             complement(47..1906)
                     /gene="gss"
                     /locus_tag="KKO15_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBD6360627.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2111..2977
                     /gene="yghU"
                     /locus_tag="KKO15_001821"
     CDS             2111..2977
                     /gene="yghU"
                     /locus_tag="KKO15_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBD6360628.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="KKO15_001822"
     CDS             complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="KKO15_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBD6360629.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3482..3823)
                     /gene="hypA"
                     /locus_tag="KKO15_001823"
     CDS             complement(3482..3823)
                     /gene="hypA"
                     /locus_tag="KKO15_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD6360630.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3816..4304)
                     /gene="hybE"
                     /locus_tag="KKO15_001824"
     CDS             complement(3816..4304)
                     /gene="hybE"
                     /locus_tag="KKO15_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBD6360631.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4297..4791)
                     /locus_tag="KKO15_001825"
     CDS             complement(4297..4791)
                     /locus_tag="KKO15_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBD6360632.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4791..6494)
                     /gene="hybC"
                     /locus_tag="KKO15_001826"
     CDS             complement(4791..6494)
                     /gene="hybC"
                     /locus_tag="KKO15_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBD6360633.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPLEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6491..7669)
                     /gene="hybB"
                     /locus_tag="KKO15_001827"
     CDS             complement(6491..7669)
                     /gene="hybB"
                     /locus_tag="KKO15_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBD6360634.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7659..8645)
                     /gene="hybA"
                     /locus_tag="KKO15_001828"
     CDS             complement(7659..8645)
                     /gene="hybA"
                     /locus_tag="KKO15_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBD6360635.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8648..9766)
                     /gene="hybO"
                     /locus_tag="KKO15_001829"
     CDS             complement(8648..9766)
                     /gene="hybO"
                     /locus_tag="KKO15_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBD6360636.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9955..10242)
                     /locus_tag="KKO15_001830"
     CDS             complement(9955..10242)
                     /locus_tag="KKO15_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBD6360637.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(10361..11248)
                     /locus_tag="KKO15_001831"
     CDS             complement(10361..11248)
                     /locus_tag="KKO15_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD6360638.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11405..12445
                     /gene="gpr"
                     /locus_tag="KKO15_001832"
     CDS             11405..12445
                     /gene="gpr"
                     /locus_tag="KKO15_001832"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBD6360639.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12485..12979)
                     /locus_tag="KKO15_001833"
     CDS             complement(12485..12979)
                     /locus_tag="KKO15_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBD6360640.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13170..14054
                     /gene="yghA"
                     /locus_tag="KKO15_001834"
     CDS             13170..14054
                     /gene="yghA"
                     /locus_tag="KKO15_001834"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD6360641.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14326..14751)
                     /gene="exbD"
                     /locus_tag="KKO15_001835"
     CDS             complement(14326..14751)
                     /gene="exbD"
                     /locus_tag="KKO15_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBD6360642.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14758..15492)
                     /gene="exbB"
                     /locus_tag="KKO15_001836"
     CDS             complement(14758..15492)
                     /gene="exbB"
                     /locus_tag="KKO15_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD6360643.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15744..16931
                     /gene="metC"
                     /locus_tag="KKO15_001837"
     CDS             15744..16931
                     /gene="metC"
                     /locus_tag="KKO15_001837"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBD6360644.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17071..17730
                     /locus_tag="KKO15_001838"
     CDS             17071..17730
                     /locus_tag="KKO15_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6360645.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17770..18669)
                     /gene="yqhC"
                     /locus_tag="KKO15_001839"
     CDS             complement(17770..18669)
                     /gene="yqhC"
                     /locus_tag="KKO15_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBD6360646.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18863..20026
                     /gene="yqhD"
                     /locus_tag="KKO15_001840"
     CDS             18863..20026
                     /gene="yqhD"
                     /locus_tag="KKO15_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBD6360647.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20131..20958
                     /gene="dkgA"
                     /locus_tag="KKO15_001841"
     CDS             20131..20958
                     /gene="dkgA"
                     /locus_tag="KKO15_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBD6360648.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21158..22084
                     /locus_tag="KKO15_001842"
     CDS             21158..22084
                     /locus_tag="KKO15_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBD6360649.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22135..22392
                     /gene="yqhH"
                     /locus_tag="KKO15_001843"
     CDS             22135..22392
                     /gene="yqhH"
                     /locus_tag="KKO15_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBD6360650.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22435..24654)
                     /locus_tag="KKO15_001844"
     CDS             complement(22435..24654)
                     /locus_tag="KKO15_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBD6360651.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24765..26177)
                     /gene="ftsP"
                     /locus_tag="KKO15_001845"
     CDS             complement(24765..26177)
                     /gene="ftsP"
                     /locus_tag="KKO15_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBD6360652.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKDDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26252..26989)
                     /gene="plsC"
                     /locus_tag="KKO15_001846"
                     /gene_synonym="parF"
     CDS             complement(26252..26989)
                     /gene="plsC"
                     /locus_tag="KKO15_001846"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBD6360653.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27223..29481)
                     /gene="parC"
                     /locus_tag="KKO15_001847"
     CDS             complement(27223..29481)
                     /gene="parC"
                     /locus_tag="KKO15_001847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBD6360654.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30027..30509)
                     /locus_tag="KKO15_001848"
     CDS             complement(30027..30509)
                     /locus_tag="KKO15_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBD6360655.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30562..30954)
                     /gene="ygiW"
                     /locus_tag="KKO15_001849"
     CDS             complement(30562..30954)
                     /gene="ygiW"
                     /locus_tag="KKO15_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBD6360656.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31106..31765
                     /gene="qseB"
                     /locus_tag="KKO15_001850"
     CDS             31106..31765
                     /gene="qseB"
                     /locus_tag="KKO15_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBD6360657.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31762..33036
                     /gene="qseC"
                     /locus_tag="KKO15_001851"
     CDS             31762..33036
                     /gene="qseC"
                     /locus_tag="KKO15_001851"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBD6360658.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33082..>33166)
                     /locus_tag="KKO15_001852"
     CDS             complement(33082..>33166)
                     /locus_tag="KKO15_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HBD6360659.1"
                     /translation="FWLYVFSLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8139 a         8881 c         8380 g         7766 t
ORIGIN      
        1 gtactaatta ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca
       61 caattaacgg ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac
      121 acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca
      181 ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccgctgg
      241 ttttgtccag cacctcttca tgatggctga ccaggtcgat attgctacca cagcgaccgg
      301 cgatcggttt cactgcgtaa cctgttttca ccaattcatc attaacagtg aaatcggtat
      361 ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc
      421 cggggatcac cgtccacagc ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga
      481 taagacgcac ttcgttttgc ggatgaccgg tacggattgg caccgcagca aactcacggt
      541 cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt
      601 taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca
      661 gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg
      721 cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac
      781 tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc
      841 ctttatagcc ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacaggagg
      901 cggagtcggc gttgtattcg taaaccttca ggccacgctc atccatgcag aaatccatac
      961 gaccagtgat catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga
     1021 ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt
     1081 gaagatacat cagatgcagc tcgttggtgg ctttaattag ctcctgctcg gcactctcgg
     1141 taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc tgcacatagg
     1201 cgttttgtag cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca
     1261 gacgcgctcc actgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt
     1321 cagtatcttc cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttaa
     1381 gggtatagca gccgttttcg accaccatct ccagctcgcg cgtccactgt tgcccttgcg
     1441 gcaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg
     1501 taatgatggc gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg
     1561 cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt
     1621 cattaaccac ttctcgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag
     1681 taaagaccac accgtaattc agaaagagaa aacggcgagc aaattcaacg cattgccact
     1741 tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct
     1801 gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca
     1861 ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa
     1921 caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta
     1981 accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa
     2041 aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag
     2101 gcaactcact atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc
     2161 cgctggcggc gcgttcgcca atatcaatcg cccgttttct ggtccgacgc atgaaaaaac
     2221 gctgcccgtt ggcaaacatc cattgcaact ttattcgctg ggaacgccga acggtcagaa
     2281 agtaacgatt atgcttgagg agctactggc gctgggcgtt actggtgcag agtacgacgc
     2341 ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc
     2401 aaactcgaag atcccggcgc tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga
     2461 atctggttcg atcctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggatct
     2521 ggcaaaacgg actgaaacgt tgaactggct gttctggtta cagggtgccg cgccgttcct
     2581 cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt acgccatcaa
     2641 ccgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac tggcgcagca
     2701 taagtttgtt gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg
     2761 caacgtggtg ctcggcggcg tgtatgatgc cgcagagttt ctggatgcgg gaagttataa
     2821 acatgtgcaa cgctgggcga aagaagtggg cgaacgtccg gcagtgaaac gtgggcgtat
     2881 tgttaaccgc accaacgggc cgctgaacga gcagttgcat gagcgccatg acgccagtga
     2941 ttttgagaca aatacagaag ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag
     3001 ccatccaggc cggataaggc gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc
     3061 gacgctgccg cgtcttatca ggcctacaaa agctaatcat cacgcgctgg taatgtcgta
     3121 atccatttgg cgcagtgcgt ctaatgtggc tttggcttca tcttcgtcga tgatgctcat
     3181 ggcaaatccg acgtgtacca gcacccactg gcccagcaga tcggcagggt taccttcgca
     3241 aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg caagctggtg
     3301 aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg ttaactccgg
     3361 ttatttcgat tgccggacaa acgcgaaaaa gaacctttag cgggtgttcc ctctcaccct
     3421 aaccctctcc ccggaggggc gaggggaccg tccgtgcgct ttttaggcgt tgtcctcagt
     3481 cttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3541 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3601 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3661 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3721 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3781 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc cctcctgcgg
     3841 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattggtgac tggcaacgaa
     3901 aggatcattc gtgcgcagtc atccgtcagg cgttggcctt cttcaatcga catgctgtgc
     3961 gaaagcggcg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4021 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4081 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4141 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4201 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4261 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4321 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4381 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4441 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4501 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4561 caggatcatc atcgttcccg gagtgttctt tttcgacaca atggcatccg caataatcag
     4621 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4681 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacgccgat
     4741 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4801 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4861 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc ggcaaccggt
     4921 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     4981 ttccaggttg atggaacaac cgcctggtag ttgctgatga taccgtcttt aataaccatc
     5041 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5101 gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5161 gcactgtatt ggttttgcag gatatcctgc aattcacagc agtgaacggt acgaccaata
     5221 atacggccca gcgtggagtg cagctgtgcc acttccagcg tgttgccagt cagtttctga
     5281 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5341 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5401 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5461 cacggcgcct gtggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5521 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5581 tcaatgtagc ctcccgggaa caggaagctg ccgtttttgc tgtcggtcgg gaattccggc
     5641 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggcg
     5701 ataaccgcgg tatcaacctt ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa
     5761 gacttgatgt acatcaggcg ctcaaggttc aacacgccca acccgtcgag gttgattggg
     5821 ttcgcgacac cacccactgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5881 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     5941 aggttcactt ccagcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6001 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6061 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6121 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6181 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6241 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6301 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6361 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6421 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6481 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6541 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6601 caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga
     6661 atgccaccaa cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6721 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6781 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6841 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cccgatgaaa atcgccagca
     6901 acacactgat ggtgttggtc agcttgacga acaggctctt ttcatccgga ccgttgccac
     6961 gcagacccgc ctgcaccagt gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7021 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccagagcgga tgcaccttgt
     7081 agcccgccga gatcatcagc gaccccattg aagactggtg catggtcggc agcagcgcac
     7141 cgagcgcgat gatgaagaac atcaccttgt tcagacgttg tagcgacacc ttccagccca
     7201 gacgttcaaa cagtgccgga gcaaactcca gagccatcac gccgatgtag atggtcatac
     7261 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7321 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7381 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7441 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa
     7501 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7561 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7621 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7681 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7741 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7801 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7861 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7921 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     7981 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8041 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8101 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8161 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8221 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8281 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8341 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8401 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8461 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8521 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8581 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8641 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8701 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8761 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8821 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata cctttttcgt
     8881 tacagccata gcacgggtga ccaatcgcca ccggccacac accgccaaca tcgcagaatt
     8941 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9001 agccttcgcg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9061 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cggtttttgt
     9121 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9181 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9241 agctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcggagcagg
     9301 aaccaatagc gataatggct gctgagcctt ccgccgcttt gcggatgtga tccacaatcg
     9361 gctcaccggc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacca
     9421 ctaacacata ctgccctttg tacttctcga gagcgttatg tttgttctct tcgacctgat
     9481 gaccgaaggc ggcggaaagc acttcgtgat attccagaga gatagtctcc agcacgaggt
     9541 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9601 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9661 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9721 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9781 ttgcgaggac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9841 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9901 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
     9961 ttcgaggcaa taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10021 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10081 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10141 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10201 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgtta
    10261 tatatcgcca tatatagcga tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10321 catacttgtt taagacgtaa tcccgtaaga gataaaccta tcaggagaga tatttatcga
    10381 accatttcag tgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg
    10441 aatcattatg gaatccgtga ttaacccccg gatagatata agcctcataa accttattat
    10501 tgtctttcaa cgccgcctcg taaggaggcc agccctcgtt gattcgggtg tccagttccg
    10561 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10621 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc
    10681 caccgccata gcaaaatccg gtaataccca ctttacctgt cgcttgcgga tagcgttgca
    10741 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    10801 gctcacgacc tttatcatca tttcccggat aacctccaac ggaacttaat ccgtcaggtg
    10861 ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca
    10921 gtccacgatt ctcatgcacc acccccacgg ctggcgtttt gccgctcatt tttgcgggtt
    10981 tcaccagata accccgtacc tcgccgtgac catttggcga aggatacgtg atgtactcag
    11041 caacaatctc cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca
    11101 aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga
    11161 actcacgttt cgaaattttc ccgtgaacgt aatagtcgta gtaatccaac aactcttgtg
    11221 ggaaatcttt ggcggttaaa cgcggcatcg ttgcattcct caactggtgt ttttttaagc
    11281 aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat
    11341 acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacgga ggaaaggaga
    11401 ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcagtacc gctattgcgg
    11461 aaaaagtggt ttacgcctgc ccgcattatc gctcggttta tggcacaatt tcggtcacgt
    11521 taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg tttgatttag gcattacgca
    11581 ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg
    11641 cctgctgcgc gaggattttg ctgcttatcg cgatgaactg attatctcta ccaaggctgg
    11701 ctacgatatg tggcccggcc cgtacggctc tggcggttca cgtaaatacc tgctcgccag
    11761 cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt actctcatcg
    11821 cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag
    11881 cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt
    11941 cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt acaatttact
    12001 gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg
    12061 tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc
    12121 gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct
    12181 taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc agcgtggaca
    12241 atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga cgtcggtatt
    12301 gattggtgcc agccgcgcgg agcaactaga ggagaacgtg caggcgctga ataatctgac
    12361 atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg agctgaatct
    12421 gtggcaggcg tcgtccgata aatgacctgt taataacggg cgcgcgaacc gcgcccgaat
    12481 cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat gcagaaagca
    12541 caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca tttttcgcat
    12601 ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt
    12661 ttttcagcga cgttgcgtcc attttcccca gccacttcag cttctctttg ttctcggaga
    12721 tatccagctg tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc
    12781 caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg
    12841 agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta
    12901 acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat
    12961 tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc
    13021 aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat
    13081 tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa
    13141 aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt
    13201 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    13261 caccgatacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta
    13321 aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc
    13381 gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    13441 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    13501 atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata
    13561 ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg
    13621 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    13681 cgatccccct gctaccgaaa ggtgcaagtg tcatcaccac ttcgtcaatc caggcatacc
    13741 agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc
    13801 gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    13861 ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    13921 gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt
    13981 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    14041 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    14101 ctcttacctg atgtggcgaa aacgccttat ccgcctacaa acgcatgaag attcaacatg
    14161 ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc acctaatgcc
    14221 ggatgcgtcg tgaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat
    14281 agattttgta ggcctgatga gcgtagcgca tcaggcaatt ctactttact tcgctttggc
    14341 ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac
    14401 cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt
    14461 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    14521 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    14581 ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc
    14641 cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat
    14701 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    14761 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    14821 caggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa
    14881 gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg ctgccacgag
    14941 gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt
    15001 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    15061 accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    15121 acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    15181 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    15241 gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgtgc
    15301 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct
    15361 cttactgaag aagattgccc aggtgactac agaggccaaa ataagcccaa tcatcacgca
    15421 cttaacgaca atatcggcgt actgatacat accccagacg gaaaggtccg tctgcattaa
    15481 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    15541 aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    15601 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    15661 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    15721 cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg
    15781 caggacgcag caaaaaatac actcttggcg cggtaaatag cgtgattcag cgcgcttctt
    15841 cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg
    15901 agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg
    15961 aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt
    16021 cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    16081 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    16141 atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc
    16201 tggaatcgcc gggttccatc actatggaag tccacgacgt tccggcgatt gttgccgccg
    16261 tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc
    16321 tgtttaaggc gctggatttt ggcatcgatg tttctattca ggccgccacc aaatatctgg
    16381 ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc
    16441 tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    16501 gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag
    16561 tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct gccctgcctg
    16621 gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct
    16681 ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat aacttcagtt
    16741 tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca aatcaaccag
    16801 aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc ttgattcgcc
    16861 tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcac
    16921 ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt
    16981 aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat aggcagataa
    17041 aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    17101 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    17161 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    17221 gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga ttttctgcct
    17281 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg
    17341 agctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    17401 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    17461 tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac
    17521 cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt
    17581 tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    17641 ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg
    17701 gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc tgctgtaccg
    17761 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    17821 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat
    17881 cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg
    17941 gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa
    18001 cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    18061 tttattctca attcgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac
    18121 cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    18181 gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc
    18241 cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga
    18301 cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc
    18361 gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    18421 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt
    18481 gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt acatcaccgg
    18541 tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gttctgacaa
    18601 actattttct ttatttttca gtttattaac tttatccgcc agcaagcgac aaatctcttc
    18661 acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg
    18721 tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga
    18781 gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt
    18841 ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct
    18901 gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt
    18961 gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc
    19021 cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac
    19081 gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc tggcggttgg
    19141 cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa
    19201 tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat
    19261 gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc
    19321 ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca catgttcagc cggtatttgc
    19381 cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta acggcgtagt
    19441 ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg ccaaaattca
    19501 ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa gatggtccga aagccctgaa
    19561 agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa
    19621 cggtttgatt ggcactggcg taccgcagga ctgggcaacg catatgctgg gccacgaact
    19681 gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg
    19741 gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa
    19801 catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt
    19861 ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg gcagctccat
    19921 cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga
    19981 cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc
    20041 aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg
    20101 ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag
    20161 gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa tgaggaagta
    20221 atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc
    20281 tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa
    20341 ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc
    20401 gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt
    20461 cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga
    20521 ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat
    20581 gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat gcaacaacgc
    20641 cagctacacg cctggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg
    20701 caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc
    20761 aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg
    20821 aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac
    20881 aaagacgaac taggcaaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct
    20941 gaccagttcg gcggctaacg tgcaaattct cccggtggcg gtaatgttcc gctaccggac
    21001 ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga
    21061 atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa caactcatct
    21121 atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt
    21181 agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg tagaacaaac
    21241 agtccggcag atttatcaga actataaatc agatgccagt acgccttatt tcggtgaaac
    21301 cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga acgacaatct
    21361 tacactaccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt
    21421 tggcgatctg gtgttagaaa gcgtagcgat aacccaaact gacgtcgatc atgccgatgc
    21481 tgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacacaga cactgaaaat
    21541 ggtggcggaa aatggtcgtt gggtcattga tgatattgtc agcaatcatg gcagcgtctt
    21601 acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt tgcagaaaga
    21661 acagccagaa tcctttgttg ctgaactctt tgaacatatt gctgattata gctggccgtg
    21721 gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata aaaccacctt
    21781 caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta tttacgacaa
    21841 tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga
    21901 gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg gcaacaatga
    21961 agagcaagag ctggttttac agcggcgcga aggtaagtgg gaaatcgctg attttatccg
    22021 cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaagca
    22081 atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa
    22141 acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat
    22201 gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg
    22261 cttgagcagg atatgaaagc actacgccca caaatctatg ctgccaaatc cgaagctaac
    22321 agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg cttgcgtatg
    22381 tacgcagaat gataaaaaaa tccccggcag catgtcagtt gccggggatt ttttttaacg
    22441 tccaaccgcc gctttagggc gtttcttcgc accagcattc accggacgag attgcgtaga
    22501 cgacgctttt tttgccgtag caggcgtctg acgctgagtc gccatcggcg tatgtttagt
    22561 caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt caatggttgg
    22621 cgcaggcact aagcaatcgc gacggctgcc aatcagatgc tttttgccca tcgcttccag
    22681 cgcctggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa
    22741 acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg ctcttataac caatcttcgc
    22801 cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg atgggtagaa
    22861 gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca aattcaccat
    22921 atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac gggatcagat actgctcttt
    22981 acctgcctgt ttcgagtagg tatcgaacag ctctttaaag cggtcatagc tgcccatgcc
    23041 cggcttcatc atcttcgaca acggcccttc ttcggtatgt tccggagcaa tcttcagata
    23101 accgccgacg tgatgggtcg ccaactcttt gatatagcgc ggatcttcca cggcgatgtc
    23161 ataacgtacg cctgaggcga taaggatttt tttaatgcct ttcagatcac gcgcacggcg
    23221 atagaggttg atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat ccggataaac
    23281 gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat ttgcagcgca acatatacat
    23341 gttggcagtt ggcccaccga gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg
    23401 gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc
    23461 gtgctcggta atagaacaga aagaacagcc gccaaagcag ccgcgcataa tgttgaccga
    23521 aaaacggatc atttcgtaag ccggaatacg ggcattgcca taggccggat gtggcacgcg
    23581 cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga tagcaggcgg
    23641 gttgatccac acatagcggt caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt
    23701 ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc actttatcgc ccttcacttt
    23761 ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt ttctggcgcg gtggctgcac
    23821 gattacagct ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg cacacggcaa
    23881 atcttcgcca tacggatgcg ggattgggtc gattttccct ggggtatcaa gacgggtgga
    23941 atccacaccg ctccagccag gcagcgcctc ttttacgata attgcggtat tacgcacatc
    24001 gcggatttca ctaatcggct cgcccatcgc cagacgatgc gctacttcca ccagcggacg
    24061 ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct accagaacgg aacggcgcac
    24121 ggtatcggac cagtaatcat aatgcgcggt acggcgcagg ctagcctcaa taccgccgag
    24181 gatcaccggc acatctttcc acgcctcttt acaacgctgg gtataaacca gcgtggcgcg
    24241 atccggtcgc ttacccgcga cattatctgg cgtgtaggca tcatcatgac gtaaacggcg
    24301 atcggcggta tagcggttga tcatcgaatc catgttgcca gcagtaacac caaaaaacag
    24361 attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct gggcgatgat
    24421 cccgacgcga aagccctgcg cttccagcat acgaccgcaa atcgccatcc cgaagcttgg
    24481 gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc agccaagttg
    24541 atccatctct tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta
    24601 ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc tcattttgct
    24661 tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac gaaagagaaa
    24721 tcgaaaggta ttccatactc gccctcctcg ggcgagtatg aagattacgg taccggattg
    24781 accaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag
    24841 aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac ttgtccgtca
    24901 acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcgcc attcacgtta
    24961 cggatctgga acattacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc
    25021 cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt
    25081 ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaattgg cgaaccagcc
    25141 atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg cagaaggccg
    25201 gttgggcgca gcgttagcac caaggtagaa accagaatac tggatggctc aaagaagccg
    25261 cgaatacgat caacaatgct cgccgcttca ccacaggtga tcgacacttc atcgccgttg
    25321 ctcatatcca ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat
    25381 acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc
    25441 atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg cacccagcca
    25501 cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag
    25561 ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg tttatcctgg
    25621 ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga cttgctgact
    25681 tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg
    25741 ggagtattgg cgtgatacca cagagtagct gcgttctggc gaataggcag tacgggtgcc
    25801 cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc tggtacctgt
    25861 agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat aagcttaaca
    25921 tcgtcgtcct tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccagacc
    25981 gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt
    26041 tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg cccggctgcg
    26101 ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga
    26161 cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa
    26221 agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgttctg
    26281 cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa tgggcagcca
    26341 gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa
    26401 tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta gagacgcaca
    26461 cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta
    26521 gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt
    26581 ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt
    26641 cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc
    26701 tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat
    26761 agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca tctgcaggtt
    26821 tgcggcactc aactttcagg ccgaacagtg gcgcaagacg gccaaacata tgcccaaagg
    26881 tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga
    26941 ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg
    27001 aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc
    27061 cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga
    27121 atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg tttcatccgg
    27181 cgttccttgc tagcgggagg aaacagcgcc ctccccggca tattactctt cgctatcacc
    27241 gctgctggca cggcgaggag agtcgatctc aacacgatcg atacgctgca aaccgcgcat
    27301 caacgtaccg cggcgtccac gttcgccagt gactttctgt agctcttccg gacgcagttt
    27361 aattttgcgt ttcccaacat gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg
    27421 cgccagacca tcctcgccac gcgcggcttc tgccgatgga atgttgataa tcttgttgcc
    27481 tttgcccttc gacagctgcg gcagatcgct taccgggaac atcaacatac ggcctgcctg
    27541 agtgattgcc agcagcatat cggaagcatc ttcaatcacc accggcggca taacatgggc
    27601 attttccggt aaggtgatca aagccttacc cgcacggtta cgcgctacca gatcgttaaa
    27661 ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc agtttctgat cgtcgctttc
    27721 catcagcata tggtcaacgg tcgccccagg cggcaacgtt aatttgccgg tgagtggttc
    27781 gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg gcatagctac gaccggtgga
    27841 atcaacaaac actaccggtt ggttactctt acctttcacc gccgctttga agctatcacc
    27901 cgctttataa ttcaggcccg gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc
    27961 catctgcgac agcacaatgg tgacaggttc agacggcagc atgtcgtgct cgctcatcgc
    28021 tttcgcttct tcgcgttcct gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc
    28081 tgcctgcagt tctttcttca gcaggttatt cattttacgc tcggaagcca aaatgccctg
    28141 caactggtcg cgctcttttt ccagctcact ctgctcaccg cgaatcttca tctcttccag
    28201 tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc
    28261 aaaccgcgac atcagcgccg gtttcggttc atcttcgtta cgaatgatct caatcacttc
    28321 gtcgatgttg agaaacgcca ccagcaaacc ttccaggata tgcaggcgct tgaggacttt
    28381 ctccagacga tagttcagtc ggcggcgcac ggtatcgcga cggaacacca gccattcgga
    28441 gaggatttcc agcaggtttt tcaccgccgg acgaccatcc agaccgatca tattgaggtt
    28501 aatgcgatag ctcttttcca gatcggtggt agcgaagagg tggttcatca cctgatccat
    28561 atccacgcgg ttggaacgcg gcacaatcac cagacgggtc gggttctcgt ggtcagattc
    28621 atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc atttgcgcag caatttgctc
    28681 cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg ataaccaccg cgccatcttc
    28741 tttcttccac accgcgcgca tacgcactga accacgtccg ttctcgtaga ttttacgaat
    28801 ctcggcgcgc gaagtgataa tttccgcttc agtcggataa tccggcccct gcacgatatc
    28861 cagcagctga tcgagcgtag ttttcggctg gtcgattaat gcgattgccg cctgagctac
    28921 ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg acggcaatac cggtggtgcc
    28981 gttaagcaaa atgtttggca gacgggcagg tagcattttc ggctcctgca aagtgccgtc
    29041 gaagtttggc acccagtcag ccgtcccctg ccccagctcg ctcaatagca gctcggaata
    29101 tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc
    29161 gccccagttc ccctgaccat caaccagcgg ataacggtaa gagaacggct gcgccatcag
    29221 gaccatcgct tcataacagg cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc
    29281 gacggtacgg gccgattttt taaatttggc gctggcattc aggcccagtt cagacatcgc
    29341 atacacaatg cggcgctgaa caggtttcag accatcacca ataaacggca acgcacggtc
    29401 catgatcacg tacatggagt agtttaagta ggcgttttcc gtaaattcat gtagcgcaag
    29461 gcgctctgcc atatcgctca ttaattctga ttcctcaact tattcgccag ccttcaaagg
    29521 ggcaatattg ccggagatac tacattatct gccgcgtcga gtcacaggag atcagcgtga
    29581 atagcgccgc cggacgtgct ccgacggcga taaaggcatc aatgtgcctt gatatacaac
    29641 tctttgctgt agacttgatc ggcatagagg gcagcacaag gttgcgccca tgccacacca
    29701 ctcggcatgg gggtccgctc cgggcgattc gagaagttga ggttatgcga gtccagggaa
    29761 gcctaaagca tcaaagtagt cgactgtaag tatacggttc agcccagttt agctgtgtct
    29821 ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc tcatggatat ccccgagacg
    29881 gcttacggcg caccggataa taaatttcgc gtatacatgg tattccttac cggagaaatt
    29941 ataagcccaa caacaatacg cctgtgacat tgttcgctca aacttatcgc gagtgatttg
    30001 gctcacactc cggtaaagat tccccatcac gccaacggca cataaacatc cgtttccagt
    30061 cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc
    30121 attttctcgc cgcttgcagg cagccagtgg cgaataatgc cccatatcgt gtgtgaaata
    30181 tcgtccagct cgccaacgtg gcgggccacg gcataacgtc caccggtaag ttcaccattg
    30241 ctcacaccat aacgattatc gggaatcggt tcgctaacgc tgccgcagat atcaaagcga
    30301 aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga cgccaaaagt ctggctttgg
    30361 ttaacaggag aaagccccgt ctctttacgc cacatgataa atttcgccac actgtagttg
    30421 agcaactcag ggctacagcg gtgcggcaac atcgccacag ggattgatgg gaaatcgata
    30481 atgtttacat ccagtgtcag gtttgtcatt ttgcctccct gcgaaattat tcccgggaac
    30541 gcgtcccggg agcggtaaca attacggatt tactttgcgg atctgtttga cgtcaatttc
    30601 aacagaattc cagtctttat cgacttcacc ctgaatctca accgtatctt tcggcgtcac
    30661 cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg atagtaccgc tggcatcttt
    30721 aaacacgtag agatcgtcag agatgcgttc aacgatattg ccgcgcaggg ttacccaggt
    30781 gtcgtcacgc agggattttg cgctttctac agtcgttacg ctgccgttcg gcccctgaaa
    30841 tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa ccgccctgct ctgctgccat
    30901 caccggtgca ctgcacaggg ccattactgc gattactgct gcgaattttt tcatgtttat
    30961 tactcccttt aatgtctgtt tccgagcatt taacaagata gtccttaaca acttcttaag
    31021 ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca acacggttta ctggcagcaa
    31081 atacggttat cgcagggatg aaaaaatgcg aattttactg atagaagatg acatgctgat
    31141 tggtgacggc atcaaaactg gccttagtaa aatgggtttt agtgtcgact ggtttacaca
    31201 aggtcgtcag ggaaaagagg cgctttatag cgcaccttat gatgcggtga tcctggattt
    31261 aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa tggcgagaga aaggtcagcg
    31321 tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg gaacgtgtag aagggctgcg
    31381 tctgggagct gacgattatc tgtgtaaacc ttttgcgttg atagaagtcg ccgccaggct
    31441 ggaggctctg atgcgccgaa ccaacggcca ggccagcaac gagctgcgcc acggtaacgt
    31501 catgctcgac cccggcaaac gtatcgccac gctggctggc gaacccttaa cactgaaacc
    31561 aaaagaattt gccctgctgg aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa
    31621 actgattgaa gagaaactgt atacctggga cgaagaggtc accagtaatg ccgttgaagt
    31681 gcatgtgcat catctgcgac gcaaactcgg tagtgatttt attcgtaccg tgcatggtat
    31741 tggctacaca ttaggtgaga aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg
    31801 acgctaatct ttttaattct ggcctcggtg acctggctgc tttccagctt tgtcgcctgg
    31861 aaacaaacaa cggataacgt cgatgaattg ttcgacaccc aactgatgct gtttgccaaa
    31921 cggttaagta cgctcgatct caacgaaatc aacgcggcgg atcgcatggc acagacgcca
    31981 aataaattaa aacacggtca tgttgatgac gatgcgctga cctttgccat ctttacccac
    32041 gacggcagaa tggtccttaa tgatggcgat aacggagaag atattcccta tagctatcaa
    32101 cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg atccttggcg ttttgtctgg
    32161 atgacctcac ctgatggcaa atatcgcatc gttgttggcc aggaatggga ataccgtgaa
    32221 gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt
    32281 atgttaatca tcatgatggt actactgggt cgtgaactcg cgccgctgaa caaactggcg
    32341 ctggcactac gtatgcgtga ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc
    32401 agcgaagtgc ggccactggt tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg
    32461 atggttcgag aacgacgctt tacctccgat gcagctcacg aactacgtag cccgttaaca
    32521 gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg atgatccgca ggcgcggaaa
    32581 aaagcactgc tccaattaca ttccgggatc gatcgcgcta ctcgtctggt tgatcaactg
    32641 ctcacgctat cgcggctgga ctcactggat aaccttcagg acgtcgcgga gatcccgctt
    32701 gaagatctcc tacaatcgtc ggtgatggat atttaccaca cggcgcagca ggcgaaaatt
    32761 gacgtgcgac tgacactcaa tgcccacggc atcaaacgca ccggacaacc gctattgcta
    32821 agtttgttgg tgcgaaattt gctggataac ggccccggtg tgacaccaga ggcactggcg
    32881 cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg ctaccggcag tggacttggg
    32941 ctatcgattg tccagcgaat cgccaagctg catgacatga atgttgagtt tgggaatgcg
    33001 gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc tctgtctatg agagccgtta
    33061 aaacgactct catagatttt actaatagca aaatataaac cgtccccaaa aaagccacca
    33121 accacaaacc agacaaataa cgagcgagaa tacatatagc caaaac
//
LOCUS       DAECOD010000039        33054 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.039, whole genome shotgun sequence.
ACCESSION   DAECOD010000039 DAECOD010000000
VERSION     DAECOD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.039"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(63..203)
                     /locus_tag="KKO15_001853"
     CDS             complement(63..203)
                     /locus_tag="KKO15_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360660.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDSTVSKTAVILTILT
                     AT"
     gene            299..1015
                     /gene="arcA"
                     /locus_tag="KKO15_001854"
     CDS             299..1015
                     /gene="arcA"
                     /locus_tag="KKO15_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBD6360661.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1074..2426)
                     /gene="creD"
                     /locus_tag="KKO15_001855"
     CDS             complement(1074..2426)
                     /gene="creD"
                     /locus_tag="KKO15_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBD6360662.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2484..3908)
                     /gene="creC"
                     /locus_tag="KKO15_001856"
     CDS             complement(2484..3908)
                     /gene="creC"
                     /locus_tag="KKO15_001856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBD6360663.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3908..4597)
                     /gene="creB"
                     /locus_tag="KKO15_001857"
     CDS             complement(3908..4597)
                     /gene="creB"
                     /locus_tag="KKO15_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBD6360664.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4610..5083)
                     /gene="creA"
                     /locus_tag="KKO15_001858"
     CDS             complement(4610..5083)
                     /gene="creA"
                     /locus_tag="KKO15_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBD6360665.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5294..6163
                     /gene="robA"
                     /locus_tag="KKO15_001859"
     CDS             5294..6163
                     /gene="robA"
                     /locus_tag="KKO15_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBD6360666.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6160..6807)
                     /gene="gpmB"
                     /locus_tag="KKO15_001860"
     CDS             complement(6160..6807)
                     /gene="gpmB"
                     /locus_tag="KKO15_001860"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBD6360667.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6859..7379
                     /gene="yjjX"
                     /locus_tag="KKO15_001861"
                     /pseudo
     CDS             6859..7379
                     /gene="yjjX"
                     /locus_tag="KKO15_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7464..7790)
                     /gene="trpR"
                     /locus_tag="KKO15_001862"
     CDS             complement(7464..7790)
                     /gene="trpR"
                     /locus_tag="KKO15_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBD6360668.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7880..9817)
                     /gene="sltY"
                     /locus_tag="KKO15_001863"
     CDS             complement(7880..9817)
                     /gene="sltY"
                     /locus_tag="KKO15_001863"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBD6360669.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10028..11695
                     /gene="ettA"
                     /locus_tag="KKO15_001864"
     CDS             10028..11695
                     /gene="ettA"
                     /locus_tag="KKO15_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBD6360670.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11802..11969)
                     /locus_tag="KKO15_001865"
     CDS             complement(11802..11969)
                     /locus_tag="KKO15_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360671.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12003..13235)
                     /gene="nadR"
                     /locus_tag="KKO15_001866"
     CDS             complement(12003..13235)
                     /gene="nadR"
                     /locus_tag="KKO15_001866"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBD6360672.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13256..14638)
                     /gene="radA"
                     /locus_tag="KKO15_001867"
     CDS             complement(13256..14638)
                     /gene="radA"
                     /locus_tag="KKO15_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBD6360673.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14687..15655)
                     /gene="serB"
                     /locus_tag="KKO15_001868"
     CDS             complement(14687..15655)
                     /gene="serB"
                     /locus_tag="KKO15_001868"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBD6360674.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15761..16405
                     /locus_tag="KKO15_001869"
     CDS             15761..16405
                     /locus_tag="KKO15_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBD6360675.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16433..17448
                     /gene="lplA"
                     /locus_tag="KKO15_001870"
                     /pseudo
     CDS             16433..17448
                     /gene="lplA"
                     /locus_tag="KKO15_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17480..17743
                     /locus_tag="KKO15_001871"
     CDS             17480..17743
                     /locus_tag="KKO15_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6360676.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(17904..18623)
                     /gene="deoD"
                     /locus_tag="KKO15_001872"
     CDS             complement(17904..18623)
                     /gene="deoD"
                     /locus_tag="KKO15_001872"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBD6360677.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18680..19903)
                     /gene="deoB"
                     /locus_tag="KKO15_001873"
     CDS             complement(18680..19903)
                     /gene="deoB"
                     /locus_tag="KKO15_001873"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD6360678.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19955..21277)
                     /gene="deoA"
                     /locus_tag="KKO15_001874"
     CDS             complement(19955..21277)
                     /gene="deoA"
                     /locus_tag="KKO15_001874"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBD6360679.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(21404..22183)
                     /gene="deoC"
                     /locus_tag="KKO15_001875"
     CDS             complement(21404..22183)
                     /gene="deoC"
                     /locus_tag="KKO15_001875"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBD6360680.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22441..23982
                     /locus_tag="KKO15_001876"
     CDS             22441..23982
                     /locus_tag="KKO15_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBD6360681.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            23954..24817
                     /locus_tag="KKO15_001877"
     CDS             23954..24817
                     /locus_tag="KKO15_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBD6360682.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25031..25813)
                     /locus_tag="KKO15_001878"
     CDS             complement(25031..25813)
                     /locus_tag="KKO15_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6360683.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(25810..26883)
                     /locus_tag="KKO15_001879"
     CDS             complement(25810..26883)
                     /locus_tag="KKO15_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBD6360684.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27005..27166)
                     /locus_tag="KKO15_001880"
     CDS             complement(27005..27166)
                     /locus_tag="KKO15_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBD6360685.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27283..27888)
                     /gene="osmY"
                     /locus_tag="KKO15_001881"
     CDS             complement(27283..27888)
                     /gene="osmY"
                     /locus_tag="KKO15_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBD6360686.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28281..29870)
                     /gene="prfC"
                     /locus_tag="KKO15_001882"
     CDS             complement(28281..29870)
                     /gene="prfC"
                     /locus_tag="KKO15_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBD6360687.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(29961..30638)
                     /gene="yjjG"
                     /locus_tag="KKO15_001883"
     CDS             complement(29961..30638)
                     /gene="yjjG"
                     /locus_tag="KKO15_001883"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBD6360688.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30653..31099)
                     /gene="rimI"
                     /locus_tag="KKO15_001884"
     CDS             complement(30653..31099)
                     /gene="rimI"
                     /locus_tag="KKO15_001884"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBD6360689.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31068..31481)
                     /gene="holD"
                     /locus_tag="KKO15_001885"
     CDS             complement(31068..31481)
                     /gene="holD"
                     /locus_tag="KKO15_001885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBD6360690.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31584..32615
                     /gene="rsmC"
                     /locus_tag="KKO15_001886"
     CDS             31584..32615
                     /gene="rsmC"
                     /locus_tag="KKO15_001886"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBD6360691.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32892..32978
                     /locus_tag="KKO15_001887"
     tRNA            32892..32978
                     /locus_tag="KKO15_001887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32926..32928,aa:Leu,seq:cag)
BASE COUNT         7775 a         9100 c         8454 g         7725 t
ORIGIN      
        1 attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc cctcttagcc
       61 tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt cgagtcctga
      121 gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga
      181 acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc atgcatcagg
      241 caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg tagcaaacat
      301 gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca cgttgaaaag
      361 tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca
      421 gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc cgggtaagaa
      481 cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga tgttcctgac
      541 tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag atgactacat
      601 caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac
      661 catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg
      721 ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc agtacaagct
      781 gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca aaattcagtc
      841 tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg accgtactgt
      901 agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata cgccggaaat
      961 catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag attaatcggc
     1021 tttaccaccg tcaaaaaaac ggcgcttttt agcgacgttt ttatttttca accttatttc
     1081 cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt cggcagtgaa
     1141 aacgcatacc agtctatatt acgagtcaca aacatcatgc cggccagcgc caccaccagc
     1201 acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca catcactcca
     1261 tccagcaaca acagcgcgag ggtaaacaac atgctattgc gccagccttt caataccgcc
     1321 tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca tgccacggta
     1381 aaaccgatat gttcagaaag cgccagcaag agcaaataaa acatcaccaa tgaaagcccc
     1441 accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac
     1501 gccataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc cgttaattgg
     1561 tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc ccagccggta
     1621 tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct ttgccattgc
     1681 gcctgaaaac ctgactcgct agcttcccgt ttggctggta gaaaatcacc taaaaaactg
     1741 ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg caccacagaa
     1801 agatcgccgg taccgcttaa attcagtgcc atattcagct gcaggttctg cttacgccag
     1861 tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc ggtaccgggt
     1921 tcaatggtca gtgccgttcc gttaacttca ggcgctttca ccacaccaat accacgcgca
     1981 tccccgacgc taatcacaat aaagggcttg cctaagatga tatttggcgc gttgagctcg
     2041 ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca gacctgaccg
     2101 gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat caatgactca
     2161 ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc ttcctgcacc
     2221 gtataaagct cggtcacagg aatagcgata agcggcccaa cgagtttttg cggcccgctg
     2281 gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc gacaatcact
     2341 tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa ggtggtcatt
     2401 ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc aggatacgaa
     2461 ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgtag cgaggccagc
     2521 acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca
     2581 ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg caaagagtaa
     2641 aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt atccagcacc
     2701 ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatgcaacc gctctcggga
     2761 gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa cgccggttca
     2821 gcagcaacat taacctcggt gggcataaca tgcaaagtga tgtttttttc tgccaactgc
     2881 acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacagc agtcagaacg
     2941 acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag cgcctgcatt
     3001 cgcgcatttt gtgtcagaat gttgtcagta aaacgagcca ctacttcagg cggcggacct
     3061 tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag ctcatgggtt
     3121 aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg catactttcc
     3181 agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg aacgggctta
     3241 ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt gatccaccaa
     3301 accatgcctg cgccaatcac cagtgcaatc cccaacaaaa tggcgctggc ccataaaatt
     3361 cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa
     3421 acgccaataa gccgcgagcc gcccataatc ggcgcggcaa catacatcac cgaactttcg
     3481 ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa cgttagccag
     3541 acgtcattcc agcgcgaata atcctgtcca acggctttat ttgccgaatc gaacaatact
     3601 ttgccatgcg catcggtcat atagacatga tattcattgc gcactttgtt aatgccacca
     3661 atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag ttgcccatgc
     3721 gttgggtccc cagagagcaa atcgggacgc gccagctccg caagtaacgt tgccgtgtcg
     3781 atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac aaaaatagcc
     3841 agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa ccgcatgccg
     3901 atacgcatta caagcccctc aggctatatc ccatgccgcg atgagtatta atcggtgaaa
     3961 gatcggggtt gatggcgcgc agcttggcac gcagcgtttt aatgtggata tcgacggtgc
     4021 gatcgtaggt gtcctgcgca tcttcccaca cgctatccat tagttgctgg cgggaccaga
     4081 cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta
     4141 atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa tgaccaatac
     4201 ggatgacggg agacggcgtc gagaacttct tcacccgacg cagtaaggtg cgcaccctgg
     4261 cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca atttccagcc
     4321 caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt aacgccggat
     4381 ggagcgccag taactggcgg cacaattcaa agccactgat atcaggcaaa ccaacgtcga
     4441 gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaaa cctcgctcaa
     4501 agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg gctatccctt
     4561 gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt tattgccgcc
     4621 acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca actttgtcgg
     4681 agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg acctgtaacg
     4741 acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct ttgccgtttt
     4801 taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc gcatcggagg
     4861 tatcttctgc caggcccaat ccccctttaa taccaccggt tttcgcccgg ctcacataac
     4921 aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg tgatccgggc
     4981 cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc aatggcccca
     5041 gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta ccatttttat
     5101 ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga acaaaatctc
     5161 aattctttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa cactgaatgc
     5221 taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg
     5281 atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct ggctggaagg
     5341 tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg
     5401 gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt atattcgtgc
     5461 tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga ttctggacat
     5521 cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca agaagcagtt
     5581 tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg gtattcgccc
     5641 gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc tggaagatac
     5701 gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct ctgatttccg
     5761 ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga ccattccgcc
     5821 ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt
     5881 attctatacc accgcgttag cccaggatca ggcagatggc tatgtactga cggggcatcc
     5941 ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg gaaccggcgt
     6001 gcaggagttt atcctgacgg tatacggaac gtgcatgcca atgctcaatc tgacgcgccg
     6061 taaaggtcag gatattgagc gatactaccc ggcagaagac gccaaagcgg gagatcgccc
     6121 aattaatctg cgctgtgaac tgctgattcc tatccgccgt taacgctgca gctcatctaa
     6181 tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acgacccagc cagacgccag
     6241 ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac gtaagcggcg
     6301 ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg caataccatg
     6361 gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca gtgccgcatt
     6421 gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt caacggtgcc
     6481 attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga tatgtctttt
     6541 ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt cacagccgca
     6601 ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac tgataatatg
     6661 cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct cacctttggc
     6721 ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact gcgtttcacc
     6781 gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt aacgttaatt
     6841 ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt
     6901 caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca tattgcatcc
     6961 gttgccgtcg agagcggtgt accggaacaa ccctttggca gtgaggaaac gcgcgctggc
     7021 gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt ttgggtggcg
     7081 attgaagctg gcattgatgg cgacagcact ttcagttggg tggtgattga aaacgccagc
     7141 cagcgcggcg aggcgcgttc ggcaactttg ccgttacctg cggtgattct ggaaaaagtt
     7201 cgtgaaggcg aggcgcttgg tcccgtgagt cgcgttatac cggtattgat gagattggtc
     7261 gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc agcgtctatc
     7321 accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag gcattgtagg
     7381 acggataagg cgttcacgcc gcatccgaca ttcggtgcac gatgcctgat gcgacgctgc
     7441 cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactga
     7501 cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc
     7561 gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag ctcttcgaca
     7621 atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac
     7681 ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc
     7741 tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc
     7801 cattgttagc gggggaagca aaatgactcc cccggttgta ctcgctaaag agtacgatag
     7861 catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg
     7921 gtttatcccc catgaaatag cggtagtaag catcataagc cagcacgttc ttcacataac
     7981 cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc
     8041 tgtggccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct gaggagaaaa
     8101 tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca atgttgatgt
     8161 tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag aacatcttca
     8221 ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct accggtgatt
     8281 tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga
     8341 tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg aatcgctctt
     8401 ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt
     8461 tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc ttcaccagat
     8521 tggcccactc gctacgcgcg gtattatcga gattccagta catcaactcg cgcacgcgcg
     8581 ccatctccgg cccctgagtc agggtgctgt caacattctg cggcgcttta tcaatcttca
     8641 gctcatactc ttcaccgatg cgttgtgctg caaccatcgg gtagaaacca cgctgttgta
     8701 tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc aataaatccg
     8761 cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc gccagccagg
     8821 tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg cacacggcgt tcaatcaacg
     8881 acgtggattg cgagcgcata atggcgtcat cgcgccattt cgcctgctca tcggtgacat
     8941 cattgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa
     9001 gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc attttcggca tcctgccgcg
     9061 ccacactggc aaacgccacc gccgccattt gacgggtaaa atcggtcgcg ccggttgtac
     9121 gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag
     9181 tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggcctgtg ttacccgctt
     9241 tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc
     9301 agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt
     9361 tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac
     9421 attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt
     9481 cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg
     9541 ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcagg tcatcggtga
     9601 tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt
     9661 cgaccacatc catctgtcga ttatcccagg cctgcttgat ttgcgcgtaa cggctacgct
     9721 gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag
     9781 ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat
     9841 acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg
     9901 ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact gggattaggc
     9961 ggggaaacag gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa
    10021 gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa
    10081 acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct
    10141 gggtctgaac ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga
    10201 catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc
    10261 gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt cagaagtggt
    10321 taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt
    10381 tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca
    10441 taacctgaac gtacagctgg agcgtgcggc ggatgcgctg cgtctgccag attgggacgc
    10501 gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct
    10561 ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt
    10621 ggcgtggctg gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga ttactcacga
    10681 ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa ctggaccgcg gtgaaggtat
    10741 tccgtgggaa ggtaactact cctcctggct ggagcagaaa gatcagcgtc tggcgcagga
    10801 agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca
    10861 gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa
    10921 cagcaccgaa taccagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg
    10981 tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg atcgcctgct
    11041 gattgatgac ctgagcttct cgatcccgaa aggggcgatc gtcgggatca tcggtccgaa
    11101 cggcgcgggt aaatcgaccc tgttccgtat gatctccggt caggaacagc cggacagcgg
    11161 caccatcact ttgggtgaaa cggtgaaact ggcatcggtt gatcagttcc gtgactcaat
    11221 ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta tgaagatcgg
    11281 caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca
    11341 gggtaaacgt gttggcgaac tttccggtgg tgagcgcggt cgtctgcatc tggcgaagct
    11401 gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga
    11461 aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc
    11521 gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc aggatgaagg
    11581 taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaaa aacgcacgct
    11641 gggcgcagac gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt
    11701 aaaatgccgg atgcggcgcg aacgccttat ccggcctaca aaaccagcaa tttcaatacg
    11761 ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa
    11821 tgccggatgc ggcgtgaaca ccttttccgg tctacaacag ccttagcatc ctcaatttcg
    11881 tggcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga
    11941 gaggttttca actcatcaaa aaaactcatt ttatacctcg cctttgagca gtttcgtcac
    12001 ggttatcctt gctcccccat catctcccgc accagctcca cgcagcgcag gaaacggctg
    12061 tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag catctccacc
    12121 agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa accatccgcc
    12181 acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc atcaatcagc
    12241 gcctgcacga acggatgctc acgcccttcg tactttttgc agaacgcctg ggtagtgaca
    12301 aaatcggtgt caataaacgc cactttattg gcgtatttca ctgcaaaatc aatgtattgt
    12361 gcgtggccca acgcgatttt atcgtagtcg gaatactgca atgcgatctc atcaccgccg
    12421 aggtgtgaaa agacataatc gcggccatat tcccacgcac tggtggtgtt gaagatattg
    12481 gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc cgccaaggat cgccacggta
    12541 cgcacaaaaa acggcttcac ttcggtagga atatattccc agtagcggaa cgggttttcg
    12601 cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag caccgtctcg
    12661 atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta gatcaggtcc
    12721 ggctggatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac atcccagcca
    12781 tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tacggatatt tttctgatat
    12841 ttaaaggttt gcaatagcca gcgcagacga tccggcaccg tgggctgctg cgacatggcg
    12901 ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat atgcagctcg
    12961 tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag cgggtagaac
    13021 ttaccgaata cgacaccaat ggttttcttc tgccggggaa attcaagccc caaaaaacgg
    13081 tgcaatgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag taactggctt
    13141 aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca gccctgttgc
    13201 ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc tctcattata
    13261 agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc tgcatccctt
    13321 ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg
    13381 ccgcttcaga aatccgctcc tggccactgg gcaccgggcg gatctcccct gccagcccga
    13441 cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg gaaaccatcg
    13501 ccagcagtaa cgctaagtcg gcgctggttt cggttacctt cacgccgccg accacgttca
    13561 caaacacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca gccagcagga
    13621 ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc attatcgagt
    13681 gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac accaccatca
    13741 cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa gggttgctga
    13801 cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc gcgccgaagc
    13861 ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc atcgccatcc agaagcaccg
    13921 aacagtcgat acagtgttcc agcactttcg ggccagccag cgaaccatct ttggttacgt
    13981 gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa acgcgtcaga taagccgccg
    14041 tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata tgcatcacct
    14101 ggatcgagtc aattaccatc agcttcggtt gctcttcttc ggcaatcagg cagatctgct
    14161 cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg cgatgagcgc
    14221 gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc atctgctggg
    14281 ccagtttgca cagcgtttgc agcaacagcg tggatttccc cgcaccaggg ttaccgccaa
    14341 tcagaatggc acttcctggc accacgccgc ctcctagtac gcggtcgaac tctttaaatc
    14401 cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg acttttgcca
    14461 ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag
    14521 caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc ccctgccagc
    14581 gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct tttgccacgg
    14641 tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc aggcaattac ttctgattca
    14701 ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg gtgacttccg
    14761 ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct gctttgatca
    14821 tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg atttcatact
    14881 cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc gtctacgatg tcgccgatca
    14941 cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg gctgtcaggc
    15001 gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca atcgccactt
    15061 tccagcccag cgtttccagc ttgagcacca gctgcgttaa gcctggcatc agcggcagat
    15121 tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc acgcggctgc
    15181 gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttacc tccgccacca
    15241 tctcgcccgt tccggccagc ttggcaattt catcaataca ttcaatctgg atggcggtgg
    15301 aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc cccagcgggg
    15361 cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg tgcggtgagt gaacctgcca
    15421 gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc gccgcaccca
    15481 gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac agcagccagc
    15541 cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat gaaagaggca
    15601 gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg
    15661 ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg tatccattgc
    15721 ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca caaaactgaa
    15781 attccggcta catcgggcag tgattgtcct gttctgtctt gccttgttag tggcactgat
    15841 gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac agctggaaga
    15901 actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga tgcgtaccga
    15961 ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacagatg aaagccgtat
    16021 cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg gcgaaagcgt
    16081 cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca ggcggctatt ttaaccagca
    16141 gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc tgacactcga
    16201 cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca ttttacgcct
    16261 gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc tacagggtaa
    16321 acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga
    16381 ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg ttatgtccac
    16441 attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg tggaagagtg
    16501 tatttttcgc caaatgcccg ccacgcagcg cgttctgttt ctctggcgca atgccgacac
    16561 ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc ggatggaaga
    16621 agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc gcggtgttcc acgatctcgg
    16681 caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacta tctccacgtc
    16741 gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc gaagcgtccg gacgtaacga
    16801 tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca ggctcggcct atcgcgaaac
    16861 caaagatcgc ggcttccacc acggcacctt gctactcaat gccgacctca gccgcctggc
    16921 aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattactt cagtgcgttc
    16981 ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc atgagcaggt ttgcgaggcc
    17041 ataaccgagg cctttttcgc ccattatggc gagcacgtgg aagcggaaat catctccccg
    17101 gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag tagctgagag
    17161 tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt tagctggggc
    17221 ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca ggtatttacc
    17281 gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg ctgcctgtac
    17341 cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc ggaccaggaa
    17401 aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact cgctgattaa
    17461 aatatccaag gaaggaaata tgatcgatct ggaaaaccag gaacgggaga ttatcaattt
    17521 aatgttcagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata aattatcgct
    17581 tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaacaga tcgaaaagac
    17641 ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa atttatggtt gtccaccaga
    17701 gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt cgtgaataga
    17761 tacttgaatg ttttttattt tctatccata tgaaaaataa tagtaaatct ataaatcagt
    17821 tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc ttaacacgac
    17881 aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga ttccagtgcg
    17941 attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg
    18001 cggatgtggt cagacacggt gcagatggtc agggctttcg cgccaaactc tgcagccacg
    18061 ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc catcacgtcg
    18121 aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc cacgcgagca
    18181 tcaacgccca gtgctttagc tgcatctact gcgttacgca ccatgtcaaa gtcagcgata
    18241 gcggcaaagt catggtcttt aaaacggatg cggttaactt tggaatcggt gcaggccccc
    18301 ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca ggaaccaacg
    18361 cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat ggagcaggac
    18421 gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt accagtgaag
    18481 cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt ttcagcaata
    18541 tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc gcccatttct
    18601 gcattaatgt gtggggtagc cattgtttta tcctttttgt gacataacaa aggcggagtc
    18661 aacgctccgc ccaaatccat cagaacatgg ctttgccata ttccatatca gaagtaccaa
    18721 aatattttgc cagagtctgg ccgatatccg cgaaggtttc acggtgaccc agtgagcccg
    18781 gttttacttt cgggccatat accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg
    18841 tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag gatgtcgtca tcgcgcagca
    18901 gagacatcag ctccggcaga cggcggtcga acagttccag acccgcagca taaccggcga
    18961 cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa gttggtgaag acgatggtgt
    19021 tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc aagccagtcg
    19081 ctttcacttt tttggtgata ccgcagttgg cgtagatgtc tgcaatttta ccgacagaaa
    19141 ccacctggcc gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa
    19201 cagccaagtc gtgacggtta ccggtacgct ggaagttacc agctttgtca ccgataaacg
    19261 gacgagcgat aacacgaccg atattgtagc cgccgttggt cagctcttca cgggcgattt
    19321 cgcacagttc gtagagttta tccagaccga aagtttcttc atggcaggca atctggaaca
    19381 cggagtcagc ggaggtatag aaaatcggct tgccggtttt catgtgctct tcgcccagtt
    19441 gatccagaat gaccgtaccg gaagagtggc agttaccgag gtaacccggc agattagcgc
    19501 gttcgaccag tttatccagc agctcttgcg ggaagctgtt ttcgtgatcg gagaaatatc
    19561 cccactcaaa cagaaccggg acaccggcga tttcccagtg accagacggg gtatctttac
    19621 cggatgacat ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg ccgtccattc
    19681 ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc cagccccaga cgggtcagat
    19741 ttggcagatt gagcggccct ttacgaccat tatcagcttc gcctttggca caagcttctg
    19801 cgatatgacc cagggtgtca gccccgacgt caccaaagcg ttctgcatct tctgtagcgc
    19861 cgatgccgaa tgagtccagc accataataa atgcacgttt catatgttct ccgtactcag
    19921 tgcttcaaat ttaaatgatc agatcagtat accgttattc gctgatacgg cgatagacag
    19981 ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc cgctttcacc gctttcgccg
    20041 cttcctgcca gctgttttcg tctttcgcga ggataaccgc cagcggacgc tgaccgtcta
    20101 cctggtcgcc cagacgcgcc atatcagtaa agccgacgct gtaatcgatg gtgtcagatg
    20161 cctgacgacg accgccgccc attgcaacca ctgccatccc cagcgcgcgg gtatccattt
    20221 cactgacaaa accttcggta tcagcataga ctgctttcgt cagcatcgct gtcggcagat
    20281 acttcgcgta gttctcaacg aagtcggtcg ggcctttttg tgccgctacc atacgaccaa
    20341 agacttctgc cgctttaccg ttgtccagca ccgcctgcaa tttcgcgcgc gcttcggcgt
    20401 catctttcgc cagtttgccg gagatcagca tctccacgca cagcgccatc gtgacatcaa
    20461 acagacgcgg gttacgatac tcacccgtca ggaactgcac cgcttcacga acttcaaccg
    20521 cgttacctgc actggaggcc agtacctgat tcatgtcggt gagcagcgcg gtggtgcgca
    20581 cgccagcgcc gttagccacg ccaacaatcg cttcggcaag ggcttcagag agttcgtagg
    20641 tcggcataaa cgcgccgcta cccactttca cgtccatcac cagcgcgtcc agaccttccg
    20701 ccagtttctt cgccaggatc gaggcagtga tcagcgggat ggagtccacg gttgcggtaa
    20761 tatcacgggt cgcgtagaaa cgtttatcag ccggagccag tgaactggtc tgaccgataa
    20821 tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga
    20881 agccagggat ggattccagt ttgtcgagcg taccgccagt atgaccgagg ccgcgaccgg
    20941 agatcatcgg aatatagccg ccgcaggctg cgaccatcgg ccccaacatc agcgaagtga
    21001 catcgccgac gccgccggtc gagtgtttat caacaatcgg gccattcaga tgcaggcttt
    21061 tccagtcgag aactgttcct gaatctcgca tcgccatggt cagcgagaca cgctcaggca
    21121 ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc aatctgccct tcggaaatag
    21181 tgttgtcgcg aataccgttg ataaagaaac gaatttcttc atcgctcagc gcatgaccat
    21241 cacgtttttt acgaataatt tcttgtgcga gaaacaaggt aacctcccga ggaaaagagt
    21301 atgaggcggc ttttgccgga tgcgtctacg ccttatccgg cctacgggta gatacgtctc
    21361 gtaggcctga taagcgcagc gcatcaggcg taaagcatct tacttagtag ctgctggcgc
    21421 tcttaccgtc gccgtggccc agcgctttca gcaggcttgc cagcaggctg gaagcaccaa
    21481 agcggtagtg acgcgcatca gcccagtcag caccgaacag ttcatctgcg atggcgagat
    21541 atttctgcgc atcttccgca gtacgcacgc cgcccgccgg tttgaaacca acggtttttt
    21601 ctacgcccat atcacggatc acttccatca tgatgcgagc gctttccggc gtcgcgttca
    21661 cagccacttt accggtagag gttttgatga agtccgcacc cgctttgatg gagatttcag
    21721 acgctttacg gatcagcgct tcgtctttca gttcgccggt ttcgatgatc actttcagca
    21781 gtacattcgc tgccgcgcaa gcctctttac aggctttcac caggtcaaaa ccaacctgct
    21841 cgtcacccgc catcagcgcg cggtacggga acaccacgtc aacttcatca gcaccgtagg
    21901 cgattgccgc acgggtttct gccagcgcga tttcgatgtc gtcgttaccg tgtgggaagt
    21961 tggttaccgt agcgatacgg atttccgggg tgccctgctc tttcagagtt ttgcgagcaa
    22021 tcgggataaa acgaggatag atacagatag cggcggtatt gccgaccgga gttttggcct
    22081 gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc attcagggtg gtcaggtcca
    22141 tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt catttcattc tcctggcttg
    22201 tcgccaataa aattcacctt gcgagtttgt tagtattcta acatctactc cgcaacacac
    22261 ttcgatacac atcacaatta aggaaatcta cttacaagtt tgcatcactg taatgcgatc
    22321 tggttcaaat aattcacttt caaatgaatg cgtcagtggt ggcaaacgca tcaggatcaa
    22381 agtgaacatc acgaaacttc ttacaatggc gcatcacctt cggcataaaa ggaaaagaag
    22441 atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc agcaaattgt caccagccca
    22501 gtgcttagcc cggagcagaa gcgccatttt ctggcactgg aagcagaaaa caatctgcct
    22561 tacccacagc ttcctgccga agcccgccgc gcgctggatg aaggtgtaat ctgcgatatg
    22621 tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac ccgattacgc tcgttttctg
    22681 gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag atcttgatga cgcactctct
    22741 ctgctgacca ttctttacca ccacgtaccg tcggtcacat cgatgccggt ctacctgggg
    22801 caactggatg cgttgttgca accgtatgtt agaattctaa cacaaaacga gatcgatgtt
    22861 cgaataaaac gtttctggcg ttacctcgac agaaccctgc cagatgcctt tatgcacgcc
    22921 aatatcggcc cgtctgattc gcccattacc cgcgcgatct tacgtgcaga cgcgcaggtt
    22981 tcaccgaacc tgacctttat ctacgatcct gaaatcaccc ctgatgacct gctgctggaa
    23041 gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat
    23101 aaaattttca caaaaggggg ctacgggatt gtgagctgtt acaactcact gccgctggcg
    23161 ggtggtggca gcacgctggt acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg
    23221 ctggatgact tctttacgcg cactctaccg cactactgcc agcagcagat cgccatcatc
    23281 gatgcgcggt gtgaattcct ctatcaacaa tcacacttct ttgagaatag cttcctggtg
    23341 aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt ttggcatgta tgggctggcg
    23401 gaagcggtta acttgctgtg tgagaaagaa gggattgccg cgcgctacgg taaagaagcc
    23461 gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg cggagtttgt cgccaatacc
    23521 cccgtgaaat atggctggca aaaacgcgcc atgttacacg cacagtcggg gatcagttcc
    23581 gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg atgagccaga tccgatcacc
    23641 catctgcaaa ctgtcgcccc gcatcatgct tattattatt ccggcatcag cgacattctg
    23701 acgctcgacg aaaccatcaa acgtaacccg caggcactgg tacagctttg cctcggtgcc
    23761 tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt
    23821 accggttata tggtgcgttt gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc
    23881 aacaccacct ggctgggcga agaagccgca cgcaacactc gtattctgga acgccagccg
    23941 cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt aagattatcc ccttctcctg
    24001 cgttgacggg ccaggcagtc gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa
    24061 aaactgtcac aatccgtgga cgatgggacg ttgcaatgac tgtggggagt gcgtgccaca
    24121 gtgtccgcat caggcgttgc agattgttga cggcaaagtg gtgtggaacg ctgtggtctg
    24181 cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat gccacaccaa tggcgcaatc
    24241 catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg gtgctgttta ttgaagggat
    24301 aacggtaagc ggcggcgaag ccacgaccca actgccgttt gtagtggcgc tgtttactgc
    24361 tatcaaaaac gatccgcaac tgcgccatct cacctgtctg gtggacagta acggtatgtt
    24421 gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac ggcgcaatgc tcgatctcaa
    24481 agcatggggt agcgaatgtc atcaacaatt aaccggacgc gataatcaac agattaagcg
    24541 cagcatctat ttgctggcag agcgcggcaa gctggcggaa ctgcgtttgc tgatgatccc
    24601 aggccaggtg gattatttgc aacacatcga agaactggcg gcgtttatca agggacttgg
    24661 tgatgttccg gttcgcctga acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag
    24721 ctgggcgagc gccacgccgg aagacgttga gccgttggct gatgcattaa aggtgcgcgg
    24781 ggtgagccgg ttgatatttc cggcgctcta tttgtgacat gccggatacg gcctacggtt
    24841 cggcacagac ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca ttgtgcgcca
    24901 actgccggat gcggcgtgaa cgccttatcc ggcctacggt tcggcacaaa cctgtaggca
    24961 tgataagacg cgttaagcgt cgcatcaggc attatgcgcc gactgccgga tgctgcctac
    25021 gaagggcaaa ctacccacga acgttaaaca ccgcatacgt gttattaagc aacacttcgg
    25081 caatctcatc cgcgggttcc gggcgtaact cgcaaagcac ggcaaacaca cgggcagcct
    25141 gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg catatccggc gcatcggttt
    25201 ccagcaacaa cgatgccagc ggtaattttg cgatgacatc gcgggtttta ctggcgcgtg
    25261 gataggtgat agtaccgcct acgccgattt tgtagcccag ctgtacaaac cgttcagcct
    25321 gttgctggct gccagaaaat ccgtggacaa caccagcacg cggcaaatcg tggtgtttaa
    25381 gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag gatcaccggc agatcgtagc
    25441 gtttcgccag tttcagttgt tcgtcgagta accactgctg cctctcaaat tgcggatcgt
    25501 cgccgaagag atccagaccg atctccccca ccgccaccac cttcgccgga cgcctttcca
    25561 gcgcctgctg tagctgctca agagacacat cgctatgttt ttccaacata ccaggatgca
    25621 agcccaatgc ggcatacagc ggttgataat tttccgctaa cgcctgcacg cgggcaaaat
    25681 tttccgcctc tgtcgccgga acaatgatct tgcctacgcc cgcttgtgcc gcgcgttgca
    25741 ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa atggcagtga gtgtcgataa
    25801 aacggcaaat caagccagat cctcattgtt aaatgtcggg tcgttcgcct gcggagcgtc
    25861 gctcacttcg gcaaccagcg tatcgttggc gactggcgca ggcggaatga caatcgattc
    25921 cggcgtcacc actggcacca gatggcgggt aagcggcgct ttttcagtta atagcttgcc
    25981 aaccgtggcg aggaaataac gaccgcataa acgcccaagt ttatagtctt cacgcagcgc
    26041 cggaatccga ctgccaagcg cgatactatg taatggcttc ggcggataaa tttcggatat
    26101 ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg tcacgatagc tggtttcatg
    26161 atgctgcacc agattgacca gcggctgcag gctactgtca cccagccagc gttccatgcg
    26221 tttgaaccac tgcggcgtgt agtacatttg tgacggcaca gtgcggatga cgaccaacgt
    26281 tttagcgccc tgccttgccg cctctttaac cggaatcgca tcactgatcc cgccatccag
    26341 gtagttaatg ccttccagcg acactccgct acgataaaag ccaggtatcg cactggaggc
    26401 gcgaatcaca tccagccagt tttgtttggt tggtaaaaag taattcggcg cgtagtcatc
    26461 ctgacgacag gcgcacatat aaaacgattt gccgctgtca aacaaccgcg cggcggtatc
    26521 catttgcaac ggcatctggc ttgcggtggc ctccaccagc cagtcgagat cgataagatt
    26581 tcctccacgg acaaagcgca atggatcgaa aaattcgcgt tttgtggtat agcgcatgat
    26641 gactttgcgc gcgtaaccgg gctgattgca gataaacgcc gagaggttct gcgccccggc
    26701 agatgtgccg agataaagat cgaaaggatt aaactgcgcg cgcataaact catccagcac
    26761 gccagccgtg aaaattccac gctgtccgcc gccttcgcac accagagcta ttcgtccagg
    26821 ctggaacggc cttagcgaca acggcgcaat attaccaagc gttacaggta ttcgctgccc
    26881 cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa gtaacgcaat ttattattcg
    26941 ctaaaaccgc aaaagccagt ccgctggact ggctttggat aaaattttct taatggtctg
    27001 aaatctaggg tcgttttcgg cccatgaaca aactcactag gaacagaata atcccgacga
    27061 caaagacaat tttagctgcg cctgcagcgg taccggccag accaccaaac ccaagtgcgg
    27121 cggcgattaa cgcgataacc agaaatatga tgccccaacg aaacataagc gtctccttta
    27181 ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca ctcaatgcga tatttttagt
    27241 gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg aattacttag ttttcagatc
    27301 atttttaacg cttttcacac catctaccgc tttggcgata ctttcagcac ggtcactttg
    27361 tgcctgagaa tctacggtac cggagagctg aaccacgcca tcggtggttt caactttcac
    27421 tttacgtgaa gggacgatat cgtccgccag cagtttggct ttgatttcac tggtggtggc
    27481 ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg tcgcgaacgt gcagtttgtc
    27541 gctgacagag gtcacgcctt caacgccttt cgccactttc actgcctctt cagcctgggc
    27601 ctgactttca acgaaaccgc tcagggtcac gactttttga tcggttttga cagagatatc
    27661 ggtgctcttg atgttgtcat gatccaccag cgccgccttc actttcgcgg tgatggcgct
    27721 gtcatccatg aaattaccga ctttattcat agagctatcg actttttgcc ctgcgctttc
    27781 attggtagtc tgcgcgttgt tttccgcgta ggcagagccg gtcgcgacgg cagaggtcaa
    27841 cattacagcc agcagagttt tcgaaatctt cagtcttgtc atagtcatcg atttattcct
    27901 gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg cattgctgaa tagggagaga
    27961 cttcaccctc tacagaagtc aatggtcgcc atcacaaaag cgatgggtga tgaataacga
    28021 ccattacagc ctctgaatca gttattaata tcggtagaat gacaatcgac ggctttagat
    28081 actgatatct acgcattgaa cggtatttaa cgccatcaga aatgtcatca ctttgttaaa
    28141 tatagatcac aattttgaaa ccgctcggga tatcagcgaa aacataagca aaagtgaatg
    28201 ttttaagaac attccgtaag cggctaataa ggaagggaaa ttgacagggc gcagcggcta
    28261 ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt ggaactgaac gtccggataa
    28321 cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga tgtaagcgag gttatcgccg
    28381 ccatcaagcg ccagttggct ttcgttctta cgcttgaact cttcgaattt cttcgcgtct
    28441 gcacattcta cccagcgggc agtggcaacg ttgactgact catacactgc ttcaacgttg
    28501 tattcgctct tcaggcgcgc taccaccaca tcaaactgca gcacaccaac cgcaccaacg
    28561 atcagatcgt tgttggagat tggacggaac acctgcaccg cgccctcttc ggaaagctgt
    28621 accagccctt tgagcagctg tttttgcttc agcggatctt tcaggcggat acgacggaac
    28681 agttctggtg cgaagttcgg aataccggtg aacttcatca tctcaccctg ggtaaaggtg
    28741 tcgccgatct gaatggtgcc gtggttgtgc aggccgagga tatcgcccgg atacgcttct
    28801 tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt cggagatcac cacatctttc
    28861 gcagtgcgca cctggcgcag tttcatgcct ttttcatatt taccggacac cacacgcata
    28921 aacgccacgc ggtcgcggtg tttcggatcc atgttggcct gaattttaaa tacgaagccg
    28981 gtaaatttat cttcgctcgc ttctacggta cgggtatcgg tctgacgcgg catcggcgca
    29041 ggcgcccact ccaccaggcc attcagcata tgatcaacgc cgaagttacc cagcgcagta
    29101 ccgaagaata ccggagtgat ttcgcccgca aggaacagct ctttgtcgaa ctcgttagac
    29161 gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg ccagatcttc accaaccgca
    29221 gcatcgagat ccgggttatt cagcccttta acaatgcgga cttcctgaat ggtgtgccct
    29281 ttaccgctct gatagagata ggtttcatct ttataaaggt ggtaaacgcc tttaaacagc
    29341 ttgccgcagc caattggcca agtgatcggt gcgcagccaa ttttcagctc gttctcaact
    29401 tcatcgagca gttccatcgg gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg
    29461 atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac gggtacgatc ttcaacacct
    29521 tttgcggcgt cgataaccat caggcagcag tccaccgccg tcagggtacg ataggtatct
    29581 tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa ccaggcaatc gtgatacgga
    29641 aactgcatca cagacgtagt aatggagatc ccacgctgct tttccatctc catccagtcc
    29701 gacttagcgt gctggttgga accacggcct tttactgtac cggcggtctg aatggcctgt
    29761 ccgaacagca gcaccttctc ggtgatggta gttttaccgg cgtccgggtg agaaataatg
    29821 gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag acaacgtcat aattctttct
    29881 tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga aaaattgcgg ctattttacc
    29941 catcaacggg ggggaggcaa tcagtgttta cacaggagct gctccagttc gtgcaacgaa
    30001 gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc gattgtgcgc attcagccag
    30061 caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg actcggcagt gtcgccaacc
    30121 atcagcacgc gtgaacggtc aggattgccc gcctgttcca gcgcataatc gaaaattttc
    30181 ttattcggtt tggcaacgcc aacttcttcg gaaatcacca gcaaatcgaa gtaatcacgc
    30241 aggcccgtcc gttccagacg cacttgctgc aaggcgctaa aaccgttggt gatgatgccg
    30301 attttagcgt tgctacgaat ggcgttaagc agagaaaccg cgcccggcaa cggcgtacag
    30361 atttccgcca tcgcattaat aaaggcatcg ttaagcaagc ctgcttcaac tttcagccgt
    30421 tcggcccagc tctcgaaacg cccgtgctga agctgtaatg aagtgatcgc gccgttttga
    30481 taatccaccc acagtggctt gttaacggcc tgatagtcct gaaaatcttc agcggtaaag
    30541 gtgacgctgt aatcaagaaa catccgctgc aggccggtga atgagtcaaa ggtaaacagc
    30601 gtttcatcgg catcaaagaa aatccagtcc cacttcatta ttccaccttg tcttacatac
    30661 tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc cgtggtgggg tagtaattgc
    30721 ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag ggcaatggcg gcagcatttg
    30781 aggcacggac ttccagccat agtgtcgcca cgccgcgttt ttccagttcg tcgatcagat
    30841 gttccagcag cgccctaccc agcccctgac gctgatagtc gggatcgacc gcaatattga
    30901 acaatgtagc ttcatccagc accacttgcg taatcgcaaa cgccgccatt ttgccgtttt
    30961 gcgttaactg aaagttgaga taacgctcgc cctggttgct ggcaaaggtt ttttcactcc
    31021 acggaaaggc gtgggcgcgt tgttcaatgt ggtaagccgc tggtaaatca gtcgtttcga
    31081 gggaagaaat cgtgttcata tgtgcaaatt tgttgccata acgcggcgcg tgccgttggg
    31141 tttgcccgta attcggtgag cgccggtgat gccacctgag cgccttccaa tgatagcggt
    31201 tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt gcggcagcat cgcgattttt
    31261 tctggcgtca gttgcagcac ttgatcgggg ctgacggtta atgcgcgcag aacatcgctc
    31321 actaaaggat cagtcagggc gggaagatcg tttgccacca tcaccagacg gacgtgtgcc
    31381 gggctcgcaa tggcaatctc cccctgcaac gcgccagggc gacgcagcga ccactgggta
    31441 atgcccagtt gctgtaactg ccagtctcgt cgggatgtca tagcgaatcg ctcctgttgt
    31501 caggggcgca aatatagcaa attcgtcgat accgcgccaa catatggcta taatcgccgc
    31561 cagtatcaat tgaggagcat tccatgtctg catttacccc ggcaagtgaa gtcttgctgc
    31621 gtcacagtga tgatttcgaa caaagccgta ttctgtttgc cggagactta caggatgacc
    31681 tgcccgcgcg tttagatacc gcggccagcc gtgctcatac ccagcaattc caccattggc
    31741 aggtgttaag ctgccagatg ggggataacg cccgttttag tctggtcgcc acggcgaatg
    31801 atgtcgcaga ttgcgatacg ctgatttact actggccgaa gaacaaaccg gaagcccagt
    31861 tccagttgat gaatttactt tctctgctgc cagtggggac agatattttt gtcgttggcg
    31921 agaaccgcag cggcgtgcgc agcgctgagc agatgctggc agattatgcg ccgttgaata
    31981 aagtcgacag cgctcgtcgc tgtggcctct attttggtcg tctggaaaaa cagccagtat
    32041 ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg cctgacggtc aaaacgctgc
    32101 ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca gttgctgctc tcgacgttaa
    32161 ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg cgcgggtgta ctttcagttg
    32221 cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg cgatgtctct gccccggcgg
    32281 tagaagccag ccgcgcaaca cttgcagcca acggtgttga aggtgaagtc tttgccagca
    32341 acgtcttttc cgaggtgaaa ggttgttttg atatgatcat ctccaacccg ccgttccacg
    32401 atgggatgca aaccagcctg gatgcggcgc aaacgctgat tcgtggcgcg gtgcgtcatc
    32461 ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt cctgccttac ccggacgtgc
    32521 tggatgagac atttggcttc catgaagtta tcgcgcaaac cgggcgcttc aaggtatatc
    32581 gcgccattat gacccgccag gcgaagaaag gttgagtatc tgatatctgt cgccgcatac
    32641 cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa acctgcacgt tagcccttcg
    32701 taggccagat aagacgcgcc agcgtcgcat ctggcatctg cccccgcatg cctaccaaac
    32761 ctgcacgcta gtttcctgat ggacattttt ccagcaatta cacatcaggc gataattaac
    32821 tattgacgaa aagctgaaaa ccactagaat gcgcctccgt ggtagcaatt ctttttaaga
    32881 attgatggta tgcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgtcc
    32941 ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa aaccacgttg atattgctcg
    33001 cactgggcga aggtggcgga attggtagac gcgctagctt caggtgttag tgtt
//
LOCUS       DAECOD010000040        31126 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.040, whole genome shotgun sequence.
ACCESSION   DAECOD010000040 DAECOD010000000
VERSION     DAECOD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.040"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            389..2047
                     /locus_tag="KKO15_001888"
     CDS             389..2047
                     /locus_tag="KKO15_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBD6360692.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="KKO15_001889"
     CDS             complement(2044..3000)
                     /locus_tag="KKO15_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBD6360693.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="KKO15_001890"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="KKO15_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBD6360694.1"
                     /translation="MKKRKTVKKRYVIALVIVIAELITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="KKO15_001891"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="KKO15_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBD6360695.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KKO15_001892"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KKO15_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBD6360696.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="KKO15_001893"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="KKO15_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBD6360697.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="KKO15_001894"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="KKO15_001894"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBD6360698.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9799..9886)
                     /locus_tag="KKO15_001895"
     tRNA            complement(9799..9886)
                     /locus_tag="KKO15_001895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9850..9852),aa:Ser,seq:gga)
     gene            complement(10140..10358)
                     /gene="infA"
                     /locus_tag="KKO15_001896"
     CDS             complement(10140..10358)
                     /gene="infA"
                     /locus_tag="KKO15_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBD6360699.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10643..11347)
                     /gene="aat"
                     /locus_tag="KKO15_001897"
     CDS             complement(10643..11347)
                     /gene="aat"
                     /locus_tag="KKO15_001897"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBD6360700.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="KKO15_001898"
     CDS             complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="KKO15_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBD6360701.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="KKO15_001899"
     CDS             complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="KKO15_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBD6360702.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="KKO15_001900"
     CDS             complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="KKO15_001900"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBD6360703.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNTTQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16509..17003
                     /gene="lrp"
                     /locus_tag="KKO15_001901"
     CDS             16509..17003
                     /gene="lrp"
                     /locus_tag="KKO15_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBD6360704.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTPVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17138..21166
                     /gene="ftsK"
                     /locus_tag="KKO15_001902"
     CDS             17138..21166
                     /gene="ftsK"
                     /locus_tag="KKO15_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBD6360705.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21321..21932
                     /gene="lolA"
                     /locus_tag="KKO15_001903"
     CDS             21321..21932
                     /gene="lolA"
                     /locus_tag="KKO15_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBD6360706.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21943..23286
                     /gene="rarA"
                     /locus_tag="KKO15_001904"
                     /gene_synonym="mgsA"
     CDS             21943..23286
                     /gene="rarA"
                     /locus_tag="KKO15_001904"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBD6360707.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23377..24669
                     /gene="serS"
                     /locus_tag="KKO15_001905"
     CDS             23377..24669
                     /gene="serS"
                     /locus_tag="KKO15_001905"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBD6360708.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24909..27353
                     /gene="dmsA"
                     /locus_tag="KKO15_001906"
                     /pseudo
     CDS             24909..27353
                     /gene="dmsA"
                     /locus_tag="KKO15_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27364..27981
                     /gene="dmsB"
                     /locus_tag="KKO15_001907"
     CDS             27364..27981
                     /gene="dmsB"
                     /locus_tag="KKO15_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD6360709.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27983..28774
                     /locus_tag="KKO15_001908"
     CDS             27983..28774
                     /locus_tag="KKO15_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD6360710.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28810..29436)
                     /locus_tag="KKO15_001909"
     CDS             complement(28810..29436)
                     /locus_tag="KKO15_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6360711.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29751..30899
                     /locus_tag="KKO15_001910"
     CDS             29751..30899
                     /locus_tag="KKO15_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6360712.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7671 a         7634 c         8357 g         7464 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc gaactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9721 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9781 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9841 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9901 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
     9961 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10021 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10081 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10141 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10201 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10261 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10321 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10381 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10441 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10501 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10561 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10621 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10681 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10741 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10801 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10861 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10921 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    10981 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11041 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11101 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11161 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11221 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11281 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11341 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11401 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11461 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11521 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11581 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11641 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11701 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11761 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11821 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11881 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    11941 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12001 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12061 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12121 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12181 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12241 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12301 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12361 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12421 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12481 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12541 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12601 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12661 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12721 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12781 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12841 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12901 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    12961 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13021 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13081 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13141 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13201 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13261 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13321 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13381 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13441 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13501 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13561 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13621 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13681 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13741 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13801 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13861 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13921 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    13981 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14041 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14101 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14161 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14221 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14281 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14341 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14401 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14461 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14521 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14581 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14641 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14701 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14761 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14821 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14881 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    14941 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15001 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15061 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15121 acgccaggaa tgctggtctg ggtggtatta ccatgaatac ccgactgtac tttgatgtag
    15181 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15241 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15301 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15361 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15421 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15481 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15541 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15601 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15661 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15721 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15781 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15841 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15901 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    15961 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16021 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16081 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16141 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16201 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16261 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16321 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16381 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16441 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16501 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16561 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16621 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16681 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16741 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16801 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16861 aaacacccgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16921 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    16981 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17041 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17101 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17161 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17221 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17281 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17341 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17401 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17461 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17521 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17581 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17641 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17701 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17761 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17821 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17881 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    17941 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18001 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18061 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18121 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18181 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18241 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18301 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18361 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18421 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18481 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18541 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18601 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18661 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18721 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18781 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18841 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18901 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    18961 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19021 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19081 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19141 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19201 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19261 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19321 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19381 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19441 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19501 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19561 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19621 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19681 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19741 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19801 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19861 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19921 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    19981 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20041 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20101 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20161 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20221 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20281 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20341 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20401 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20461 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20521 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20581 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20641 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20701 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20761 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20821 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20881 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    20941 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21001 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21061 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21121 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21181 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21241 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21301 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21361 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21421 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21481 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21541 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21601 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21661 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21721 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21781 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21841 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21901 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    21961 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22021 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22081 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22141 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22201 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22261 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22321 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22381 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22441 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22501 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22561 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22621 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22681 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22741 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22801 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22861 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22921 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    22981 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23041 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23101 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23161 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23221 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23281 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23341 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23401 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23461 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23521 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23581 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23641 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23701 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23761 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23821 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23881 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    23941 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24001 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24061 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24121 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24181 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24241 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24301 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24361 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24421 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24481 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24541 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24601 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24661 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24721 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24781 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24841 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24901 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    24961 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25021 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25081 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25141 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25201 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25261 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25321 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25381 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25441 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25501 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25561 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25621 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25681 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25741 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25801 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25861 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25921 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    25981 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26041 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26101 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26161 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26221 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26281 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26341 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26401 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26461 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26521 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26581 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26641 gcgttgagca acagtttact gaaggccgta cccaggaaga gtggatgcgg catctgtatg
    26701 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26761 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26821 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26881 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    26941 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27001 caggtttcca ctataaatct cgcgttcact caacttacgg caacgttgat gtgctgaaag
    27061 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27121 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27181 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27241 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27301 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27361 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27421 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27481 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27541 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27601 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27661 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27721 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27781 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27841 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27901 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    27961 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28021 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28081 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28141 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28201 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28261 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28321 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28381 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28441 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28501 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28561 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28621 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28681 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28741 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28801 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28861 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28921 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    28981 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29041 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29101 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29161 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29221 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29281 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29341 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29401 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29461 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29521 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29581 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29641 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29701 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29761 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29821 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29881 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    29941 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30001 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30061 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30121 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30181 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30241 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30301 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30361 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30421 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30481 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30541 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30601 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30661 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30721 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30781 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30841 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30901 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    30961 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31021 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31081 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcag
//
LOCUS       DAECOD010000041        30666 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.041, whole genome shotgun sequence.
ACCESSION   DAECOD010000041 DAECOD010000000
VERSION     DAECOD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.041"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="KKO15_001911"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="KKO15_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD6360713.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="KKO15_001912"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="KKO15_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBD6360714.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="KKO15_001913"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="KKO15_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBD6360715.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="KKO15_001914"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="KKO15_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBD6360716.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="KKO15_001915"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="KKO15_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBD6360717.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="KKO15_001916"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="KKO15_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBD6360718.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="KKO15_001917"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="KKO15_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBD6360719.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="KKO15_001918"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="KKO15_001918"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBD6360720.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="KKO15_001919"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="KKO15_001919"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBD6360721.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KKO15_001920"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KKO15_001920"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBD6360722.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KKO15_001921"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KKO15_001921"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBD6360723.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KKO15_001922"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KKO15_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBD6360724.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KKO15_001923"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KKO15_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBD6360725.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KKO15_001924"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KKO15_001924"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBD6360726.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KKO15_001925"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KKO15_001925"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBD6360727.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KKO15_001926"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KKO15_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBD6360728.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="KKO15_001927"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="KKO15_001927"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBD6360729.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="KKO15_001928"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="KKO15_001928"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBD6360730.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="KKO15_001929"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="KKO15_001929"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBD6360731.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="KKO15_001930"
     CDS             22192..22782
                     /locus_tag="KKO15_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBD6360732.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="KKO15_001931"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="KKO15_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBD6360733.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="KKO15_001932"
     CDS             23254..23949
                     /locus_tag="KKO15_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBD6360734.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KKO15_001933"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KKO15_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBD6360735.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="KKO15_001934"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="KKO15_001934"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBD6360736.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="KKO15_001935"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="KKO15_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBD6360737.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KKO15_001936"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KKO15_001936"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBD6360738.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KKO15_001937"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KKO15_001937"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBD6360739.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7193 a         7984 c         8438 g         7051 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcatacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaagcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatgtcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAECOD010000042        29467 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.042, whole genome shotgun sequence.
ACCESSION   DAECOD010000042 DAECOD010000000
VERSION     DAECOD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.042"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            71..352
                     /locus_tag="KKO15_001938"
     CDS             71..352
                     /locus_tag="KKO15_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBD6360740.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1394
                     /locus_tag="KKO15_001939"
                     /pseudo
     CDS             318..1394
                     /locus_tag="KKO15_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1787..2128)
                     /locus_tag="KKO15_001940"
     CDS             complement(1787..2128)
                     /locus_tag="KKO15_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBD6360741.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2141..3013)
                     /gene="copD"
                     /locus_tag="KKO15_001941"
                     /pseudo
     CDS             complement(2141..3013)
                     /gene="copD"
                     /locus_tag="KKO15_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="KKO15_001942"
     CDS             complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="KKO15_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBD6360742.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3530..3760
                     /gene="holE"
                     /locus_tag="KKO15_001943"
     CDS             3530..3760
                     /gene="holE"
                     /locus_tag="KKO15_001943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBD6360743.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3862..4518
                     /locus_tag="KKO15_001944"
                     /pseudo
     CDS             3862..4518
                     /locus_tag="KKO15_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4542..5204
                     /gene="exoX"
                     /locus_tag="KKO15_001945"
     CDS             4542..5204
                     /gene="exoX"
                     /locus_tag="KKO15_001945"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBD6360744.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="KKO15_001946"
     CDS             complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="KKO15_001946"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBD6360745.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGTSIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7471..8130)
                     /locus_tag="KKO15_001947"
     CDS             complement(7471..8130)
                     /locus_tag="KKO15_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBD6360746.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8457..8813)
                     /locus_tag="KKO15_001948"
     CDS             complement(8457..8813)
                     /locus_tag="KKO15_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBD6360747.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="KKO15_001949"
                     /pseudo
     CDS             complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="KKO15_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9304..10482
                     /gene="purT"
                     /locus_tag="KKO15_001950"
     CDS             9304..10482
                     /gene="purT"
                     /locus_tag="KKO15_001950"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBD6360748.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="KKO15_001951"
                     /gene_synonym="eda"
     CDS             complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="KKO15_001951"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBD6360749.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="KKO15_001952"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="KKO15_001952"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBD6360750.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="KKO15_001953"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="KKO15_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBD6360751.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="KKO15_001954"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="KKO15_001954"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBD6360752.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="KKO15_001955"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="KKO15_001955"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBD6360753.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="KKO15_001956"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="KKO15_001956"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD6360754.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="KKO15_001957"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="KKO15_001957"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBD6360755.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="KKO15_001958"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="KKO15_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBD6360756.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="KKO15_001959"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="KKO15_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBD6360757.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="KKO15_001960"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="KKO15_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBD6360758.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGMVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="KKO15_001961"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="KKO15_001961"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBD6360759.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="KKO15_001962"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="KKO15_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBD6360760.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="KKO15_001963"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="KKO15_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBD6360761.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24673..25275
                     /locus_tag="KKO15_001964"
     CDS             24673..25275
                     /locus_tag="KKO15_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBD6360762.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25277..25798)
                     /gene="ruvC"
                     /locus_tag="KKO15_001965"
     CDS             complement(25277..25798)
                     /gene="ruvC"
                     /locus_tag="KKO15_001965"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBD6360763.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25833..26573)
                     /locus_tag="KKO15_001966"
     CDS             complement(25833..26573)
                     /locus_tag="KKO15_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6360764.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26602..27054)
                     /gene="nudB"
                     /locus_tag="KKO15_001967"
     CDS             complement(26602..27054)
                     /gene="nudB"
                     /locus_tag="KKO15_001967"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBD6360765.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPVAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27172..28944)
                     /gene="aspS"
                     /locus_tag="KKO15_001968"
     CDS             complement(27172..28944)
                     /gene="aspS"
                     /locus_tag="KKO15_001968"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBD6360766.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29254..>29467
                     /locus_tag="KKO15_001969"
     CDS             29254..>29467
                     /locus_tag="KKO15_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBD6360767.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7225 a         7569 c         7238 g         7435 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa
     1261 tcagaagcct ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc
     1321 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1381 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1441 caaaaaagac cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg
     1501 caactaattg attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1561 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1621 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1681 caataaaaag cgcggtgctc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1741 actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1801 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1861 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1921 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
     1981 gtcggcaagt cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     2041 aacgactcac ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca
     2101 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     2161 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     2221 acacaaacca gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac
     2281 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2341 ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2401 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     2461 gaatagccta gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2521 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2581 tcgctgttac cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg
     2641 cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2701 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2761 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2821 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2881 gcagagaacg cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca
     2941 ttgcaaagcc aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg
     3001 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     3061 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     3121 gaacaatcag ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt
     3181 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt
     3241 ttaaagtgat tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3301 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg
     3361 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3421 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3481 caggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3541 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3601 ggtggctttt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3661 gccagaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3721 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     3781 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt
     3841 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3901 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     3961 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     4021 cgacgtgccc ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca
     4081 acgacctagc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     4141 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat
     4201 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     4261 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4321 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     4381 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4441 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4501 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     4561 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4621 gaaaaatcgt caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag
     4681 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4741 tgatcccaca ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4801 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
     4861 ggccagggct caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
     4921 caccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4981 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     5041 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     5101 ttgccgaacg ctatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     5161 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagcgg
     5221 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     5281 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5341 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt
     5401 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5461 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc
     5521 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5581 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5641 caacgcccat caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc
     5701 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5761 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5821 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5881 catcaatact tgtgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5941 gaaaatgttt gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta
     6001 tccacaggtg ttcactgcgg taattcgccg catcaaaacc aggcacttct gtttgtttta
     6061 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     6121 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     6181 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
     6241 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6301 acccttccag catgatgttt tcgcgtggcg gaattaactc ttcccactgt tgctcatcac
     6361 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
     6421 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6481 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6541 gatgaatgac gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt
     6601 ctttttcttc gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga
     6661 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6721 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt
     6781 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6841 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6901 cgcgcttgtt ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt
     6961 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     7021 tttttatgta gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga
     7081 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     7141 gcaggtagtc caggactttt ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
     7201 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     7261 tgttattgtt ctttctggtt ggaaacatct tataagggtg gcaaaactca ccggggatgc
     7321 aagcgaaaca gggtcgtcat tgcttagatg atgacaggta atggcgcgga tatcgaatgt
     7381 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc ggttctggat agcccgaaat
     7441 agtcaacttc aggctatcca gagagcggaa ttattccgcc aaagtgcgtt tttgctgttc
     7501 gagatcgcgt tcaatgcctt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
     7561 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcagc tcaggaaata
     7621 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
     7681 ttgttcgatt gcctgctcaa tgagtacacg cccctgctct tccacgccgg cttcacgcaa
     7741 ttgctggtcg attgccgcac gttcattggc atcaatatga ccatcacttt tagcggcaaa
     7801 gaccagcgca aggatcaaac gttccgtacg ctcatccagc ggcgtacttt gcgcgccaaa
     7861 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
     7921 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
     7981 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctgacactaa
     8041 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
     8101 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
     8161 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
     8221 gcggggcggt aggccgttat tcaaagaaat tcgcgccagg taaggtattt actgtatatc
     8281 aagtgataag ggtattttag gtgaaagtag tttgtcgttt tgagtggcat tgtttgatgc
     8341 tgattgcccg atgcgacgct gttgcgtctt ctcaggccta cgattgtggt gcgttctggg
     8401 acggacaagg cactcgcgcc gcatccgcca acggtcaatc gaattatcag aagccattaa
     8461 cgccgctgat attccgccat tcccgctttg cagtccacgc tgacctggta atgaatatcg
     8521 gcacttttac cacgcacggt tagcggtact gaccatttat catctttacc ctgaatgtcc
     8581 tgcaaactga cccaggccac gggatcggcc tgaccgacaa ttttttgatc atctgcccag
     8641 cgcgccacgc gattttgttg ataatcacgt tttacgctcg cggcaattcc ggcagcatcc
     8701 agaccttcac actttgggaa agtgaccgac ttgctggttt cattattggc agcgaaaact
     8761 gatgcgcagg cagaaaccaa caaccgccct aaaaacgccc ctcttttttt catgtttttc
     8821 tccatagcac aatgattcag gagaaagcat ggtacaaatt gtcaggagcg caagttgctt
     8881 caggccgcgt gtgaggcatc ttcctcttcg gattcagcac cgacgacctg tggggacggt
     8941 aatttgccgg ttttcagaat ggtgctcagc acctcttttt gttctgccag ccatagcgaa
     9001 agggcttcac gttgctcgtc ttccatttgc actggagaat ttgtcagcta ggtgtcaagc
     9061 agatccgccg tatcaagcat tttgtcataa gcatcggctt cctttttgct ggtaaacgac
     9121 attttctctt cgccctcacg aatgactacg tatttaactt caaccgccat ttgcagcctc
     9181 tcataataac tgagatttta tacagtatat ttcttttcgg ttgagaaatc aacatcagca
     9241 ataaagacac acgcaaacgt tttcgtttat actgcgcgcg gaattaatca ggggatattc
     9301 gttatgacgt tattaggcac tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc
     9361 tccggtgaac tgggtaaaga agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt
     9421 gccgtcgatc gctatgccga cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt
     9481 aatatgcttg atggtgatgc attacgccgt gtggttgaac tggaaaaacc acattatatc
     9541 gtgccggaga tcgaagctat tgccaccgat atgctgatcc aacttgaaga ggaaggactg
     9601 aatgttgtcc cctgcgctcg cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc
     9661 ctggcggcag aagagctgca gctgcccact tccacttatc gttttgccga tagcaaaagc
     9721 cttttccgcg aggcggttgc cgccattggc tatccctgca ttgtaaaacc ggtgatgagc
     9781 tcttccggca aggggcagac gtttattcgc tctgcagagc aacttgctca ggcatgggaa
     9841 tacgctcagc aaggcggtcg cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag
     9901 tttgacttcg aaattaccct gctaaccgtc agcgcggtgg atggcgtcca tttctgtgca
     9961 ccagtaggtc atcgccagga agatggcgac taccgtgaat cctggcaacc acagcaaatg
    10021 agcccgcttg cccttgaacg tgcgcaggag attgcccgta aagtggtgct ggcactgggc
    10081 ggttatggtc tgtttggtgt cgagctgttt gtctgtggtg atgaggtgat tttcagtgag
    10141 gtctcccctc gtccacatga taccgggatg gtgacgttaa tttctcaaga tctctcagag
    10201 tttgccctgc atgtacgtgc cttcctcgga cttcctgttg gcgggatccg tcagtatggt
    10261 cctgcagctt ctgccgttat tctgccacaa ctgaccagtc agaatgtcac gtttgataat
    10321 gtgcagaatg ccgtaggcgc agatttgcag attcgtttat ttggtaagcc ggaaattgat
    10381 ggcagccgtc gtctgggggt ggtactggct acttcagaga gtgttgttga cgccattgaa
    10441 cgcgcgaagc acgccgccgg acaggtaaaa gtacagggtt aaacccggca aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttcccacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg agacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccagcagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat catgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctgct tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg actttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttac
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgcg cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccaaggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aatgatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcac aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagca agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcatggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggtgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctagcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgaa ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaat aattttttta tgctattacg tatattcgta tctatttcaa
    24661 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    24721 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    24781 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    24841 cccctttcaa ccatcaccac gctatctcgt tttattaaac gttcgtctaa tcaacgctat
    24901 gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag
    24961 gttccctccc ggctacgcaa actttaccac acgggtttta aatacgaatc ttcgcgccag
    25021 ttctgttcaa aatttgtctt tgatatttat aaagaggcgc tatgtattcc ggtgggtgaa
    25081 atagagacgt ttggacaact gttaaatagc aatccgaatg caaaactcac tttctggaaa
    25141 ttctggttct taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg
    25201 catcatccgg gtttggtgtt gattcacgcg gagggagttg aaacgcctca gccggaactg
    25261 accgaggcgg tataacttaa cgcagtcgcc ctctcgtcag gttcagccgc gattcgctca
    25321 tctgcatcgc attctgacta acgtggcagt gggtgatggc aatcgccagc gcatcggcgg
    25381 catccgcctg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc
    25441 ttttttcggc actacccata cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa
    25501 ataccggcaa ttcctgattc accgccgcaa caatcgccac gccgcgcgcc tggcccagtt
    25561 tcagggctga gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct
    25621 ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat
    25681 catccacttt ggtgcgtatg catccgctac ctaggtagga cagttgccta cctacctggc
    25741 ggatgacgcc gtagccggtc acgcgcgaac ccgggtcaat gccgagaata atagccatca
    25801 cgcgtctccg ttttgctgtt tagcaggcat catcagagag tcgctgcaac ctcatcagag
    25861 atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga
    25921 cgcatcagtt tcggcgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa
    25981 acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt
    26041 tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat atgtcacaac gtcttcagca
    26101 cctgcttcca gagctgcttc catgatggtg tcttcgtcgc ctttctcgaa ggagatcacg
    26161 ccttttttgc tgaacagata agctacggaa ccgtcagtgc cgaggttacc gccacatttg
    26221 ctgaatgcgt gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc
    26281 atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca
    26341 tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt catgttgtta
    26401 gacagtgctt tatccaccgc cgcacgcaga cgcgggttag cgtccggatc gccaccgccc
    26461 agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca
    26521 tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata
    26581 tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg
    26641 gttgctccag gacttagtga gcgccgccgc aacaggcgca tcaagccact tgtaagccag
    26701 atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt
    26761 attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac
    26821 cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg taacctcttc
    26881 ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac
    26941 cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg
    27001 tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc
    27061 tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca
    27121 ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt
    27181 ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct
    27241 cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg
    27301 gatattgtcg gtgccagtca gcagcatggt cagacggtca agaccgaatg ccagacctgc
    27361 gtgcggcgga gtaccgtatt tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc
    27421 ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg
    27481 tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc
    27541 attttccggt gcagctttca gttctgcagc agtcatatct ttcggtgagg tgaacggatg
    27601 gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca
    27661 cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggagcag
    27721 tgcgcccatc ccgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc
    27781 atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc
    27841 taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag
    27901 acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag
    27961 cgatgcgcca cccggaacac gtagagcggc aacacggcct ttcggatcgt tcgccggccc
    28021 cgcaaatact gcaaactcaa cagatttcag cagatcggca acgtcggtca gttccatcgg
    28081 gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa aggtcattac
    28141 cgggaaatcg cccagatcca cgcccttcac ttccagccac aaatgacgca ccagcgcttc
    28201 catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa
    28261 ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga tctgatagta
    28321 acggtcaaaa ccggacatca tcagcagctg tttgaacaac tgtggggatt gcggcagtgc
    28381 gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt
    28441 ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg
    28501 caccaggctg gtgattttag cgcgggtttt caggcgctgc gccatttccg ggcgacgcag
    28561 gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg
    28621 cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcaa tttcgccggt
    28681 cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg taacctgaat
    28741 gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa
    28801 aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc
    28861 acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag
    28921 ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc
    28981 ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg
    29041 cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc
    29101 gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg
    29161 cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt
    29221 gtgaggcacg attttgtttt ttcaggagtc agcatgcttg aacttaatgc taaaaccact
    29281 gcgttggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact
    29341 gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag
    29401 cccgtgtttc tggtgcgagt tggctggtct gccgattacg ccgaagcatt aaaacagccg
    29461 gttgatg
//
LOCUS       DAECOD010000043        29061 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.043, whole genome shotgun sequence.
ACCESSION   DAECOD010000043 DAECOD010000000
VERSION     DAECOD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.043"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..156
                     /locus_tag="KKO15_001970"
     CDS             <1..156
                     /locus_tag="KKO15_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6360768.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="KKO15_001971"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="KKO15_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBD6360769.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3066
                     /gene="uidA"
                     /locus_tag="KKO15_001972"
     CDS             1363..3066
                     /gene="uidA"
                     /locus_tag="KKO15_001972"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBD6360770.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="KKO15_001973"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="KKO15_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="KKO15_001974"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="KKO15_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBD6360771.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="KKO15_001975"
     CDS             complement(5627..7135)
                     /locus_tag="KKO15_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD6360772.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="KKO15_001976"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="KKO15_001976"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBD6360773.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="KKO15_001977"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="KKO15_001977"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD6360774.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="KKO15_001978"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="KKO15_001978"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBD6360775.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="KKO15_001979"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="KKO15_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBD6360776.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="KKO15_001980"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="KKO15_001980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBD6360777.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVSPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="KKO15_001981"
     CDS             complement(14284..15603)
                     /locus_tag="KKO15_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBD6360778.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="KKO15_001982"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="KKO15_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBD6360779.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="KKO15_001983"
     CDS             16700..17035
                     /locus_tag="KKO15_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBD6360780.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="KKO15_001984"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="KKO15_001984"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBD6360781.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="KKO15_001985"
     CDS             complement(17791..19173)
                     /locus_tag="KKO15_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6360782.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /locus_tag="KKO15_001986"
     CDS             complement(19359..20303)
                     /locus_tag="KKO15_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6360783.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDSTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="KKO15_001987"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="KKO15_001987"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBD6360784.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="KKO15_001988"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="KKO15_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBD6360785.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="KKO15_001989"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="KKO15_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBD6360786.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="KKO15_001990"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="KKO15_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBD6360787.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="KKO15_001991"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="KKO15_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBD6360788.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="KKO15_001992"
     CDS             complement(25948..26769)
                     /locus_tag="KKO15_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBD6360789.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="KKO15_001993"
     CDS             complement(26869..26952)
                     /locus_tag="KKO15_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360790.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27380)
                     /locus_tag="KKO15_001994"
     CDS             complement(27045..27380)
                     /locus_tag="KKO15_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBD6360791.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27777..29030)
                     /locus_tag="KKO15_001995"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="KKO15_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         7458 a         6922 c         7415 g         7266 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcaggcaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa cactcttgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggcat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgcaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgcca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacta
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc tatccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccacgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgtgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat c
//
LOCUS       DAECOD010000044        29041 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.044, whole genome shotgun sequence.
ACCESSION   DAECOD010000044 DAECOD010000000
VERSION     DAECOD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.044"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="KKO15_001996"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="KKO15_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBD6360792.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3181
                     /gene="mdtO"
                     /locus_tag="KKO15_001997"
     CDS             1139..3181
                     /gene="mdtO"
                     /locus_tag="KKO15_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBD6360793.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3178..4644
                     /gene="mdtP"
                     /locus_tag="KKO15_001998"
     CDS             3178..4644
                     /gene="mdtP"
                     /locus_tag="KKO15_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBD6360794.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4842..5081
                     /locus_tag="KKO15_001999"
     CDS             4842..5081
                     /locus_tag="KKO15_001999"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360795.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5091..5261
                     /locus_tag="KKO15_002000"
     CDS             5091..5261
                     /locus_tag="KKO15_002000"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD6360796.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5310..6722
                     /gene="fdhF"
                     /locus_tag="KKO15_002001"
     CDS             5310..6722
                     /gene="fdhF"
                     /locus_tag="KKO15_002001"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBD6360797.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6723..6989
                     /locus_tag="KKO15_002002"
     CDS             6723..6989
                     /locus_tag="KKO15_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBD6360798.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7083..7772
                     /locus_tag="KKO15_002003"
     CDS             7083..7772
                     /locus_tag="KKO15_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD6360799.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7850..9163)
                     /gene="gltP"
                     /locus_tag="KKO15_002004"
     CDS             complement(7850..9163)
                     /gene="gltP"
                     /locus_tag="KKO15_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBD6360800.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9505..10101)
                     /gene="nrfG"
                     /locus_tag="KKO15_002005"
     CDS             complement(9505..10101)
                     /gene="nrfG"
                     /locus_tag="KKO15_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBD6360801.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10098..10481)
                     /gene="nrfF"
                     /locus_tag="KKO15_002006"
     CDS             complement(10098..10481)
                     /gene="nrfF"
                     /locus_tag="KKO15_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBD6360802.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10474..12192)
                     /locus_tag="KKO15_002007"
     CDS             complement(10474..12192)
                     /locus_tag="KKO15_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBD6360803.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12212..13168)
                     /gene="nrfD"
                     /locus_tag="KKO15_002008"
     CDS             complement(12212..13168)
                     /gene="nrfD"
                     /locus_tag="KKO15_002008"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBD6360804.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13165..13836)
                     /gene="nrfC"
                     /locus_tag="KKO15_002009"
     CDS             complement(13165..13836)
                     /gene="nrfC"
                     /locus_tag="KKO15_002009"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBD6360805.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13833..14399)
                     /gene="nrfB"
                     /locus_tag="KKO15_002010"
     CDS             complement(13833..14399)
                     /gene="nrfB"
                     /locus_tag="KKO15_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBD6360806.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14444..15880)
                     /gene="nrfA"
                     /locus_tag="KKO15_002011"
     CDS             complement(14444..15880)
                     /gene="nrfA"
                     /locus_tag="KKO15_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBD6360807.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16273..18230
                     /gene="acs"
                     /locus_tag="KKO15_002012"
                     /pseudo
     CDS             16273..18230
                     /gene="acs"
                     /locus_tag="KKO15_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18430..18744
                     /locus_tag="KKO15_002013"
     CDS             18430..18744
                     /locus_tag="KKO15_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBD6360808.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18741..20390
                     /gene="actP"
                     /locus_tag="KKO15_002014"
     CDS             18741..20390
                     /gene="actP"
                     /locus_tag="KKO15_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBD6360809.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20568..21860
                     /locus_tag="KKO15_002015"
     CDS             20568..21860
                     /locus_tag="KKO15_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD6360810.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22014..23663)
                     /locus_tag="KKO15_002016"
     CDS             complement(22014..23663)
                     /locus_tag="KKO15_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBD6360811.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23814..25163)
                     /gene="ghxP"
                     /locus_tag="KKO15_002017"
                     /gene_synonym="yjcD"
     CDS             complement(23814..25163)
                     /gene="ghxP"
                     /locus_tag="KKO15_002017"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBD6360812.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25869..26333)
                     /gene="soxR"
                     /locus_tag="KKO15_002018"
     CDS             complement(25869..26333)
                     /gene="soxR"
                     /locus_tag="KKO15_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBD6360813.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26419..26742
                     /gene="soxS"
                     /locus_tag="KKO15_002019"
     CDS             26419..26742
                     /gene="soxS"
                     /locus_tag="KKO15_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBD6360814.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26745..28322)
                     /gene="pdeC"
                     /locus_tag="KKO15_002020"
                     /pseudo
     CDS             complement(26745..28322)
                     /gene="pdeC"
                     /locus_tag="KKO15_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28751..29032
                     /locus_tag="KKO15_002021"
     CDS             28751..29032
                     /locus_tag="KKO15_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBD6360815.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7285 a         7775 c         7350 g         6631 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg cgcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtattcagt cgaacgcgtt ttacaccaaa tttgtggcga tcttgtttgt
     1381 ggttgccacg gtgctggaga tcggcagcct gtttttgatc tacaaatggt catacggcga
     1441 accgttgatc cgattgatca tcgccgggcc gatcctgatg ggctgcatgt ttttgatgcg
     1501 cacccatcgg ctgggactgg tctttttcgc tgtcgccatt tacgggcaaa ccttccccgc
     1561 catgctcgac tatccggaag tagtcgtgcg cttaacgctg tggtgtatcg ttgttggcct
     1621 ctatccaacc ttgctgatga cgttaatcgg cgtgctgtgg tttcccaatc gtgccattac
     1681 gcaaatgcat caagcgctta atgatcggct tgatgatgcc attagccacc tgacggacag
     1741 cctcgcaccg ctacccgaaa cgcggattga aagagaggcg ctggcgctgc aaaaactgaa
     1801 tgtcttttgt ctcgcggacg atgccaactg gcgaactcaa agcgcatggt ggcaaagctg
     1861 cgtggcaacg gtaacctaca tttactcgac gctgaatcgc tacgatccca cctcttttgc
     1921 tgattctcag gcaattattg aatttcggca aaaattagct tcagaaatca acaagctgca
     1981 gcatgccgtt gctgaaggtc agtgctggca aagcgactgg cggatcagtg aaagtgaagc
     2041 ggtggcggca cgggaatgta acctggagaa tatctgccag acgttgttac aactgggcca
     2101 gatgaacccg aatacgccgc caacgcccgc cgccaaaccg ccatcaatgg tcgccgatgc
     2161 ttttaccaat ccagactata tacgctacgc ggtaaaaaca ttgctcgcct gtttgatctg
     2221 ttacaccttt tacagcggcg tggactggga aggcattcac acctgtatgc tgacctgcgt
     2281 gatcgtcgct aatccaaatg tcggttcgtc gtaccagaag atggtgctgc gttttggcgg
     2341 ggccttttgc ggcgcgattc tggcgctgtt attcacgcta ctggtcatgc cctggctgga
     2401 caatattgtc gaattgctgt ttgtgctggc accgattttc ctgttggccg catggattgc
     2461 caccagctct gaacgctctt cttatatcgg cacacagatg gtggtcacct tcgcgctcgc
     2521 cacgctcgaa aacgtttttg gcccagtgta cgacctggtg gaaattcgcg atcgcgccct
     2581 gggtatcatc attggtaccg tggtgtccgc gatgatttac acctttgtct ggcctgaaag
     2641 tgaagcgcgc acgctgccgc aaaaactggc tggcgcgctg ggtatgttaa gtaaagtaat
     2701 gcggatccca cgccagcagg aagtcacggc tctgcgcact tatctgcaaa ttcgtatcgg
     2761 tctgcatgcg gcgtttaatg cctgtgaaga gatgtgccaa cgcgtggcgc tggagcgtca
     2821 actggacagc gaagaacgcg ccttactgat tgaacgttcg caaacggtta ttcgtcaggg
     2881 ccgcgatatc cttcacgcct gggatgccac ctggaattcg gcgcaggcgc tggataacgc
     2941 actacagccg gacagagcag gtcagtttgc cgacgccctg gagaaatacg ctgccggtct
     3001 ggcaaccgca ctcagccgtt ctcctcaaat aacgcttgaa gagacaccca cctcgcaggc
     3061 catcctgccc accttattaa aacaggagca acacgtctgc cagcttttcg cccgcttgcc
     3121 agactggaca gccccggcat taacgcccgc cacggaacag gcacaaggag ccacgcaatg
     3181 atcaatcgtc aactttcacg tctgctgttg tgcagcattc tcggcagcac gacgctgatt
     3241 tccggctgtg ccctggtacg taaggattct gcgcctcatc aacagctcaa accggaacaa
     3301 atcaaactgg ccgatgatat tcatcttgcc agctccggct ggccgcaggc gcagtggtgg
     3361 aaacaactca atgacccgca gttggatgcc ctgatccaac ggacgctaag tggttcacac
     3421 accctcgccg aagcgaaact gcgggaagaa aaagcgcaat cgcaggccga tttgttagat
     3481 gccggttcac agttgcaggt ggcagcgtta gggatgctta accgccaacg tgtctcggcg
     3541 aacggctttt taagccctta tgcgatggat gcgcccgcac tggggatgga cgggccgtac
     3601 tatacggaag ccacagtagg tttgtttgcc ggactggatc ttgatttgtg gggtgtgcat
     3661 cgctcagcgg ttgccgccgc cattggcgcg cataatgccg cgctggcaga aaccgcagca
     3721 gtagagctat cgctgaccac gggcgtagcg cagctttatt acagtatgca ggccagctat
     3781 cagatgctcg atctgttaga acaaactcgc gatgtgattg attacgcggt gaaagcgcac
     3841 caaagtaaag tggcgcacgg tctggaagca caagtgcctt tccacggcgc gcgggcacag
     3901 attctggcgg tcgataaaca aattgctgcc gtcaaagggc aaatcaccga aacgagagaa
     3961 tctctgcgcg cattgattgg cgcgggagcc agcgatatgc cggagatcaa acctgtggca
     4021 ttaccacgag tccagaccgg cattccggcg acactctctt atgagttgct cgccagacgc
     4081 ccggatctgc aagccatgcg ctggtatgtt caggcgtcat tagatcaggt ggattccgcg
     4141 cgggcgttgt tctatccgag ctttgatatc aaagcgtttt tcggccttga tgccatccac
     4201 ctggatacct tattcaaaaa aaccagtcgc cagttcaact tcatcccggg tctgaaattg
     4261 ccgctgtttg acggtggacg gttgaatgcc aatctcgaag gcacgcgcgc cgccagcaac
     4321 atgatgattg aacgttacaa ccagtcagta ctgaacgcgg tgcgtgacgt tgccgtcaac
     4381 ggcacgcgtc tgcaaacgct caacgacgag cgagaaatgc aggctgaaca cgtggaagca
     4441 acgcgcttca cccagcgcgc tgccgaggcc gcctatcagc gcggcttaac cagccgctta
     4501 caggccactg aagcccggtt gccagtactt gccgaagaga tgtcattact gatgctggac
     4561 agccgccggg tgatccaaag cattcagttg atgaaatcgc tgggcggcgg gtatcaggcg
     4621 gctcccgtcg tcgagaaaaa ataaaatgtc tgccgcgtga tggctgtcac gcggtatttc
     4681 gtttcgtcac gtcaaaactg acgacagcct gtttttcgtc agagttttga ataaatagtg
     4741 cccgtaatat cagggaatga ccccaaataa aatgtggcat aaaagatgca tactgtagtc
     4801 gagagcgcgt atgcgtgatt tgattaactg gagcgagacc gatgaaaaaa gtcgtcacgg
     4861 tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt cgtcgataac ggcaaaatcg
     4921 tccgggcgga ggcagcgcag gggaaaacca accagggtac cctgtgtctg aagggttatt
     4981 atggctggga tttcattaac gatacccaga tcctgacccc gcgcctgaaa acccccatga
     5041 tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg agatgaggca ctgaattacg
     5101 ttgccgagcg cctgagcgcc atcaaagaga agtacggtcc ggatgccatc cagacgaccg
     5161 gctcctcgcg tggtacgggt aacgaaacca actatgtaat gcaaaaattt gcgcgcgccg
     5221 ttattggtac caataacgtt gactgctgcg ctcgtgtctg acacggccca tcggttgcag
     5281 gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc tattaacgaa attgataata
     5341 ccgatttagt gttcgttttc gggtacaacc cggcggattc ccacccaatc gtggcgaatc
     5401 acgtaattaa cgctaaacgt aacggggcga aaattatcgt ctgcgatccg cgcaaaattg
     5461 aaaccgcgcg cattgctgac atgcacattg cactgaaaaa cggctcgaac atcgcgctgt
     5521 tgaatgcgat gggccatgtc attattgaag aaaatctgta cgacaaagcg ttcgtcgctt
     5581 cccgtacaga aggctttgaa gagtatcgta aaatcgttga aggctacacg ccggagtcgg
     5641 ttgaagatat caccggtgtc agcgccagtg agattcgtca ggcggcacgg atgtatgccc
     5701 aggcgaaaag cgccgccatc ctgtggggca tgggtgtaac ccagttctac cagggcgtgg
     5761 aaaccgtgcg ttctctgacc agcctcgcga tgctgaccag taacctcggt aagccgcatg
     5821 cgggtgtaaa cccggttcgt ggtcagaaca acgtgcaggg tgcctgcgat atgggcgcgc
     5881 tgccggatac gtatccggga taccagtacg tgaaagatcc ggctaaccgc gaaaaattcg
     5941 ccaaagcctg gggcgtggaa agcctgcctg ctcataccgg ctatcgcatc agcgagctgc
     6001 cgcaccgcgc agcgcatggc gaagtgcgtg ccgcgtacat tatgggcgaa gatccgctgc
     6061 aaactgatgc ggagctgtcg gcagtacgta aagcctttga agatctggaa ctggtcatcg
     6121 ttcaggacat ctttataacc aaaaccgcgt cggcggcgga tgtcattttg ccgtcaacgt
     6181 cgtggggcga gcatgaaggc gtgtttactg cggctgaccg tggcttccag cgtttcttca
     6241 aagcagttga accgaaatgg gatctgaaaa cggactggca aatcatcagt gaaatcgcca
     6301 cccgtatggg ttatccgatg cactacaaca acacccagga gatctgggat gagttgcgtc
     6361 atctgtgccc ggatttctac ggtgcgactt acgagaaaat gggcgaactg ggcttcattc
     6421 agtggccttg ccgcgatact tcagatgccg atcaggggac ttcttatctg tttaaagaga
     6481 agtttgatac cccgaacggt ctggcgcagt tcttcacctg cgactgggta gcgccaatcg
     6541 acaaactcac cgacgagtac ccgatggtac tgtcaacggt gcgtgaagtt ggtcactact
     6601 cttgccgttc gatgaccggt aactgtgcgg cactggcggc gctggctgat gaacctggct
     6661 acgcacaaat caataccgaa gacgccaaac gtctgggtat tgaagatgag gcattggttt
     6721 gagtgcactc gcgtaaaggc aaaattatca cccgtgcgca ggtcagcgat cgtccgaaca
     6781 aaggggcgat ttacatgacc taccagtggt ggattggtgc ctgtaacgag ctggttaccg
     6841 aaagcttaag cccgattacg aaaacgccgg aatacaaata ctgcgccgtg cgcgtcgagc
     6901 cgatctccga tcagcgcgcc gccgagcagt acgtgattga cgagtacaac aagttgaaaa
     6961 ctcgcctgcg cgaagcggca ctggcgtaat acagtccttt ctacagcctc ctttcggagg
     7021 ctgttttttt atccattcga actctttata ctggttactc cctacccaat cgtattatca
     7081 aaatgaaaaa aattatcgca ttgatgttgt ttttgacatt ctttgcccac gcgaacgact
     7141 ccgagcctgg cagccagtat ttaaaggcag cagaggccgg ggaccgacgt gcacaatatt
     7201 ttcttgccga cagctggttt agctccggcg atttgagcaa agccgaatat tgggcacaga
     7261 aagccgccga cagcggtgat gctgatgcct gcgcgctgtt ggcgcagatc aaaatcacca
     7321 atccggtcag tctggactat ccacaagcaa aagttcttgc agagaaagcg gcgcaagcgg
     7381 gcagtaaaga aggtgaagta acgctggcgc atattctggt aaatactcaa gcgggtaaac
     7441 cggattatcc aaaggcgatt tcgctgttag aaaacgcctc ggaagatctg gagaatgact
     7501 ccgccgtcga tgcccaaatg ctgcttggtt tgatttacgc caacggcgtg ggcattaagg
     7561 ccgacgatga caaggcaacc tggtatttca aacgcagctc tgcaatttcc cgaaccggtt
     7621 attccgagta ctgggcggga atgatgttct taaacggtga ggaaggtttt atcgagaaga
     7681 acaaacaaaa ggcgctgcac tggttgaacc tgagctgtat ggaagggttt gataccgggt
     7741 gtgaagagtt tgaaaaatta accaacggtt aaggtaggcc tgataagacg cggcaagcgt
     7801 cgcatcaggc atccacacat tgccggggga tatccccggc aatcttcaat tattgatccg
     7861 cagttttatc aaatttgccc agcacttcac gctcataagc cagcgccttt ttgcggtcaa
     7921 atttgtgttc ccacttggca atcaccagca ccgccagcgc attacccacc acgttcagcg
     7981 cagtacgcgc catgtcgagg atacggtcaa caccagcaat aaacgccaga ccttccagcg
     8041 gaatacctac gctacccagc gttgccagca acaccacaaa cgacacgcca gccacgccag
     8101 caatcccttt cgaggtcacc atcagcgtca gcaccagaat gatttcctgc cagatggaca
     8161 ggtcaatgcc atacagctga gcgatgaaaa tagcggcaat actttgatac agcgtcgaac
     8221 catcaaggtt aaaagagtaa ccggtcggca ccacgaagct ggtgatcgac gccggtgctc
     8281 cgtaggcttc catcttctca ataattcgcg gcagtacgct ttcagagctg gcagtggagt
     8341 acgccagaat cagctcatct ttcagaatac gaatcaggat ccagacgctt aacccgcaca
     8401 gacgtgccac aattcccagc actaccagcg cgaagaacag aatggcgaaa tgcaccagca
     8461 gcaccagttt cgccagtggc cacagagacg agaaaccaaa gttagccacc gtcaccgcaa
     8521 tcagcgcaaa cacacccacc ggtgcataac gcatcaccat gtgagtcact ttaaacatgg
     8581 tttcagagat ggagcggaac acggtcacca gcggttcacg atgcgtcgcg ggcagggaag
     8641 aaagccccag accaaacagc accgagaaaa agatgatcgg cagcatttcg cctttcgcca
     8701 tcgacgccac aatgttcgtc ggcaccagcg acaaaatcgt gcccataatg ccgtgggaac
     8761 tgctttgtac cgcttccgta gtgctctgat atttcgagat atcgacggtc gccagctgcg
     8821 acatatccac cccggcaccg ggctggaaga cgttcgccag agtgatcccc aaaatgatgg
     8881 cgacggtggt gatcacctcg aagtaaataa tggttttcgc gccaatacgc ccgagctgtt
     8941 tggcatcacc aacacccgcg atacccacca ccagcgtgga gatcacaatc ggcacaacaa
     9001 tcattttgat cagatggatg aagatatcac ccgccggaga gagcaaattg acgaccagcc
     9061 agtcgcggct gtcgctatgg tagtgcaggt agcttcccag gagaatgccc agcaccatag
     9121 caaacagaat ctgccaggcc aggctgaatt ttatattttt catcagaata acttcctcaa
     9181 tggagcgccg ttccccgttc agaaacgcga gaacgtgaca tactgccagg gcatgaattg
     9241 tgttgcgttg gtttgtagat ttttttaacg cttatgatca gtatcatttg cgcggtatgt
     9301 tgcgcaaccc tttaacaaca gagcttttcg ctgcattaat gcataataac gcgagtttat
     9361 ggcaggatta atgaataacc atttattatg gaaagatttt tatttgttta atctgcaata
     9421 atactgattg gtgattattt ttggcatatg ttcattcgat tattttttat gcagctcaat
     9481 gacataatct tcatcaccag atgattaatc cgatctccgc tgcaacagtt ttgccatatt
     9541 aatcgactcg accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg
     9601 ccatagttcg atggcttgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat
     9661 cagagcggtg atttcgttag agtccagcgc gagggctttg tcgatcattg cgcgagtctg
     9721 ggcggtcata tgctggctgg cctggtaata aagcaccgtc gccagcgccg catacagttc
     9781 agcgttctca ccacgtagtt gcaacgcctg acgatacgcc agcagcgaat tgctgtaatc
     9841 gttttgccac agatagtact cgcccagtaa cgcccattgt tcgctgtttt gtggattagc
     9901 gcggatttta tcctgcaatg cctgaagctg cgcttccggg gtttgctggc tggcaaactg
     9961 atgtagcgga tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttgggcttaa
    10021 cagataactg ccgacacaga ggaataccat caatatcgtg agtgttgtca gcattttcac
    10081 cggtatttga ggctgtttca tcgcttagcc ctcactcgcc agaggatcag tgccatcagc
    10141 aataacaaca ccactggcag cgcccacaac accagcgtct gacccgttaa cggtgggtta
    10201 tagcgaacaa aatctccgta gcgttcggtc atccagccaa tgatttcgac ttcgttcttc
    10261 ccctccgaca ccatgctata aacctgatgg cgcatactga ccgccaccgg cgcattggat
    10321 tcgagtaagt tttgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc
    10381 tgttgctgtt gttgcggatt ggcgaattgc caggtgtcta caacctgagc gcgcgcaaaa
    10441 caggtaaata acagcagtag cgtgagaagc cctttattca tcgcgcttct tcccccgcca
    10501 tccgcttaac aatgcgcccg caatcatcaa caatcctccc ccccagatcc agcgcacacc
    10561 gctttgtaca tacaaacgaa aagcgtaacg atccgctctg gttttttcac ccatgaccgc
    10621 ataccagtca tggatgccgt tccagcgaat tgacggttcc atcatttgct gacggcgtgc
    10681 ttcataaaaa cgccgctccg gcgttaattc accaatgcgt tgctgatggt caaacagcgc
    10741 cactatcgct ttttcgctgg tgtaattgcc tctggcttgc agatcgagtc gctcaaaacg
    10801 gaaggtgtat cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg
    10861 acgactgaca ctggaaacca cgatccccgc ggcaaataac agcacgccag catgagctac
    10921 cagcgccgga agctgcacgc gtttgccaga gacaaacgtc gccaacacaa tcaccaccag
    10981 catcaacaga ccaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa cggagaggcg
    11041 tccccagccc agcaggccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag
    11101 cactgcgcaa aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa
    11161 acgcaccgcc gggccaccgt cccgcgctcg ccagccatac agagccaaag acgcaaggct
    11221 aatcagtgca aacaggctga acaacggcac ggcgcggacg ttatccagtg cgaacgcatg
    11281 aaccgaaacc agaatgccag aacggacaat taaggtgccc agcagcgaca gcatcagagt
    11341 aactatcgcc agcaacagcg accagtggcg gaaaatcccc cgctggcgtg tcagggacaa
    11401 actgtgcagc agcgcagtgg cagaaagcca gggtaataaa gaggcgtttt ccaccggatc
    11461 ccagaaccac cagccgcccc agccgagttc gcagtaggcc caccaggaac cgaggatgat
    11521 ccccgccgtt aatgcactcc agccaggtaa cgcccagcgc cagcaaattc gggcgctggc
    11581 cgcatcaaac tcgccgcgca gtagactcgc cagcgccacg ctcgccgcta ccatcaaacc
    11641 gccatagcca aggtaaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg
    11701 attgagatcg cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag
    11761 caccacaaac agtagcagtg cggcgagcat gagagataaa acggctagcg tcagcggaaa
    11821 tagcggatcg ctttgctgcc gataatgcca ggcaaacagc gcgctccagg cggaaagcag
    11881 cagcacccaa agcagcagcg aaccttcatg accgccccac accgccgcca gtttgagttc
    11941 ccacgacagc aggctgtagc tatgttgggc gacataaatg accgagaaat cgctgatgag
    12001 aaaacaatac gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg ccagaatgcc
    12061 gatgcaagtg agtcgcatca tggcaggcca gcgcaaccgc actcccgcaa aggccgtcaa
    12121 cggggtcaac acgttgaccc cgagacttaa caacaacgcc agaaaaccga cttcaggaag
    12181 gaatacgtcc agaaaccacc tcttttctgg cttacgccac cgtcaactgt ccggcgtaga
    12241 gaatgaaaaa gcgcagcatc aacacgccga ccagactggc cccacacgcc gccagcacgg
    12301 caggaatgcc ggaactgcga ttgacccacg gtttgagcaa cattggcaca atcagcccca
    12361 gcccggcgac accaagccag aaccaccacg tccagaaacc gccacctaat gccgccacca
    12421 gcgcacgaac tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc accagcagga
    12481 agatttcacc ccataccacg gggatttcca tccggtgaac aaagtgcgct tccgtggaat
    12541 gcgggttact gcgttggcgt atcgccatgg cgatcagcgc caccgccgca ccggacgaga
    12601 tgccggagaa gaggagcagc accggcaggc tcgggttatt gaggaacgga tacgatttca
    12661 gcgccgacag cagaaagccg gtataagccc ccagcagcac cgccaacacc agcatcagcg
    12721 tttccagtcc gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg atccccagct
    12781 tcggcaacca gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca
    12841 gcaccagcac caccatgtag agctgaaaca gcatcacccc catcgacatc accgaggtaa
    12901 aactgtagtg gaacatcagc ttccagaagg tccacggtct tgtcaggtgg aagacgagga
    12961 tcaacagacc gaggatcacc gcgcccggcc cgacaatcag cgtggtgcgc agcaacgtac
    13021 tgtctgcacc gcccgcctgc gggtagaagc gacgtaacag cacggccagc gtcaccagac
    13081 cggcagaaat accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt
    13141 caaaatgaaa tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa acggtacgcg
    13201 gtacagcttc ggtttggttc ccagcgccag cttgtagcgg taagtaggct tctggcgcag
    13261 cagttgcgaa atctcactgt taggatcgtc cagattgcca aacgtcagcg ccttcgtcgg
    13321 gcaagcttca acgcacgcgg gaagcttacc ggcctgcaaa ttggtcttac gacagaaatc
    13381 gcatttgtcc gccgtcttcg tgaccggatg gataaagcgc acgcggtacg gacaggcggc
    13441 gatgcagtac tgacaaccga cgcagagatc aggattaaca tcgacaatcc cgctagcggc
    13501 atcacgaaaa gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg
    13561 gcaagactta cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg
    13621 aataatcgtc aggcgcgaga cgccttccgg caccttgttc acttcccgac aagcatccat
    13681 acaggcggta cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt
    13741 gatattcaac gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg ccagcacgcc
    13801 gacgccggtg agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca
    13861 gacgccgggt taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta
    13921 atccgtcctt tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag
    13981 ctggcgcacg ccactttggt tacgtggaca tcgtgcggcc agaacgcttt ttgcaactgt
    14041 tccggcagat gacaggacat acagacgctg ttctgctctc cgaccttata catcggctcg
    14101 ttaaagcgca tcacatcttt caccccttcg cggtgttgcg gtgatgcttg gccgtgacag
    14161 ttggtgcagg tgaccggcaa tttgttattc gggttgataa cggaagcatg tttgccatgc
    14221 ataccttcgg tatctggttt atgacagtcc agacaggcgg catccgggtt acgctgctgg
    14281 gtaacttcgt agcgatcacc agatgcctgc accgcagggg tagcggtaat tccgttcagg
    14341 ctccacaaca ggcccgacgc cagcacccca gcagttaata acgaacgtaa tacgctcata
    14401 ttcactccat tgtgttttcc gccccttgcg aggcggaacg gggttattgg cttaacagac
    14461 cgtttttacg tgcctgctct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct
    14521 cggccttgat ttgttccatg ttcaggccaa tggcctgctg ggctttctct ttggtcgaga
    14581 tatccgggat ctggatttca tgggtgatac ctttggtcgc cagcaggcgc gccagtttgg
    14641 tgcgcgcatc cgccgcttta tccatcgccg taccgagcat ccgtagacct tcttccggtg
    14701 cgtgcatatg aataccgtgg gaagcgatcg ccagatccca acgccactgg gcatgacgga
    14761 tatcgtcctg aatcggcttc atttcggctt ccgtcgcgcc tgcatccagc gccgctttcg
    14821 cttcgaagtg agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgatt
    14881 gcttacgttc cgcgaccact ttttgcaagg cagctttatc ctgggtatgg cagttcgcac
    14941 aagtctgggc gaagttatca aacggattac caattttatg gtcggtgtag agtttgcctt
    15001 cggcgttctg cacttttggc atatggcagt cgatacaggt cacgttgttt ttaccgtgaa
    15061 tgcccgctgt ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca
    15121 gggagttagt ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt
    15181 tcatgccgtc atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca
    15241 catggcactg accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt
    15301 taccaatggc ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc
    15361 ctttggcgaa ctctggtgag gcggtgttat ggcagtcggc acaacctaag ttgttgacga
    15421 tttccgggcc gccgcgcgcc catttaccgt ggaagtagcc atcttcgccg tctttctgga
    15481 tcagacgcgc cacatccggg cttttacaac tccagcacgc catcggtagc ggaccatctt
    15541 cagcgttttt cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca gcaaaggcat
    15601 gtccacgagg cttgttgtaa tcgcgcgaga agggataccc cgcccacagg atcaccagcc
    15661 gtggatcttc cgccagggcg tcaacacgct ctgactgttc cgaggtggct ttccaggaga
    15721 gatattgatc gtgatgctgc ggggcaaagg tttcattctt cgcttccaca gttacaggtt
    15781 ttgcgggagc tgccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata
    15841 agctgaagat acggcgtgcg tttattttta tccttgtcat aggggcttca tccgaattgc
    15901 gccattgttg caatggcggt ttttattgtt tttcacgaca gtaaccgcac ctacactgtc
    15961 atgacattgc tcacccctat gtgtaacaaa taaccacact gtgaatgttg tctttaatca
    16021 attgtaagtg catgtgaaat accactttag agttagtcag tatcttcctc tttttcaaca
    16081 gcatgcataa ctgcatgttc ctcaaaaaat taatcaactt ttgttggtga ccttcaaaaa
    16141 ttaccctgcc gtttatttgc accattctac ttttgcgtga tctgtcgccc aaatactaaa
    16201 caaaactgcc aataccccta catttaacgc ttatgccaca tattattaac atcctacaag
    16261 gagaacaaaa gcatgagcca aattcacaaa cacaccattc ctgccaacat cgcagaccgt
    16321 tgcctgataa accctcagca gtacgaggcg atgtatcaac aatctattaa cgtacctgat
    16381 accttctggg cgaacaggga aaaattctcg actggatcaa accgtaccag aaggtgaaaa
    16441 atacctcctt tgcccccggt aatgtgtcca ttaaatggta cgaggacggc acgctgaatc
    16501 tggcggcaaa ctgccttgac cgccatctgc aagaaaacgg cgatcgcacc gccatcatct
    16561 gggaaggcga cgacgccagc cagagcaaac atatcagcta taaagagctg caccgcgacg
    16621 tctgccgctt cgccaatacc ctgctcgagc tgggcattaa aaaaggtgat gtggtggcga
    16681 tttatatgcc gatggtgccg gaagccgcgg ttgcgatgct ggcctgcgcc cgcattggcg
    16741 cgatgcattc ggtgattttc ggcggcttct cgccggaagc cgttgccggg cgcattattg
    16801 attccaactc acgactggtg atcacttccg acgaaggcgt gcgcgccggg cgtagtattc
    16861 cgctgaagaa aaacgttgat gacgcactaa aaaacccgaa cgtcaccagc gtagagcatg
    16921 tggtggtact gaagcgtact ggcggaaaca tcgactggca ggaagggcgc gacctgtggt
    16981 ggcacgacct ggttgagcaa gccagcgatc agcaccaggc ggaagagatg aacgccgaag
    17041 atccgctgtt tattctctat acctccggtt ctaccggaaa accaaaaggc gtactgcaca
    17101 ctaccggcgg ttatctggtg tacgcggcgc tgacctttaa atatgtcttt gattatcatc
    17161 cgggtgatat ctactggtgc accgccgatg tgggctgggt taccggacac agttatttgc
    17221 tgtacggccc gctggcctgc ggcgcgacca cgctgatgtt tgaaggcgta ccgaactggc
    17281 cgacgcctgc ccgtatggcg caggtggtgg acaagcatca ggtcaatatt ctctataccg
    17341 cacccacggc gatccgcgcg ctgatggcgg aaggcgataa agcgattgaa ggcaccgacc
    17401 gttcgtcgct acgcattctc ggttccgtgg gcgagccaat taacccggaa gcgtgggagt
    17461 ggtactggaa aaaaatcggc aacgagaaat gtccggtggt cgatacctgg tggcagaccg
    17521 aaaccggcgg tttcatgatc accccgctgc ctggcgctac cgagctgaaa gccggttcgg
    17581 caacacgtcc gttcttcggc gtgcaaccgg cgctggtcga taacgaaggt aacccgctgg
    17641 aaggggctac cgaaggtagc ctggtgatca ccgactcctg gcctggtcag gcgcgtacgc
    17701 tgtttggcga tcacgaacgt tttgagcaga cctatttttc caccttcaaa aatatgtatt
    17761 tcagcggcga cggcgcgcgt cgcgatgaag atggctatta ctggatcacc gggcgtgtgg
    17821 acgacgtgct gaacgtctcc ggtcaccgtc tggggacggc agagatagag tcggcgctgg
    17881 tggcgcatcc gaagattgcc gaagccgccg tagtaggtat tccgcacaat attaaaggtc
    17941 aggcgatcta cgcctatgtc acgcttaatc acggggagga accgtcacca gaactgtacg
    18001 cagaagtccg caactgggtg cgtaaagaga ttggcccgct ggcgacgcca gacgtgctgc
    18061 actggaccga ctccctgcct aaaacccgct ccggcaaaat tatgcgccgt attctgcgca
    18121 aaattgcggc gggcgatacc agcaacctgg gcgatacctc gacgcttgcc gatcctggcg
    18181 tggtcgagaa gctgcttgaa gagaagcagg ctatcgcgat gccatcgtaa cccacaattg
    18241 ccggatgcga atcggtaacg gtttgtaggc ctgataagac gcgacagcgt cgcatcaggc
    18301 attgattgcc ggatgcggcg taaacgcctt atccggccta cattcggcaa gggttacccg
    18361 agcgttaacc ttctcccata agggagcggg aattaaaaca atccctacat tacctctgga
    18421 gaatctgtga tgaatggtac gatttatcag cggatagaag acaatgcgca tttcagggag
    18481 ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga ttattatgct ggcagtttat
    18541 atcggcttta ttttactgat cgccttcgcg cccggctggc tgggcacgcc gctgaatccg
    18601 aacaccagcg tcacacgcgg tattccaatt ggtgttggag tgattgtgat ctcctttgtt
    18661 ctcaccggta tctacatctg gcgggcgaac ggcgaattcg accgtcttaa taatgaagtc
    18721 ctgcatgagg tacaagcatc atgaaaagag ttctgacggc gcttgccgcc acactccctt
    18781 tcgcagctaa cgccgcggat gctattagcg gggccgtaga gcgccagcca acgaactggc
    18841 aggcgattat tatgttcctg attttcgtcg tgtttacgct cggcattacc tactgggcat
    18901 caaaacgcgt acgttctcgt agcgactact acaccgcagg cggcaatatc actggcttcc
    18961 agaacgggct ggcgattgcc ggggactata tgtccgccgc ctcattcttg gggatctccg
    19021 cgctggtgtt tacctccggc tatgacgggc tgatctactc gttgggcttc ctggtgggct
    19081 ggccaatcat tctgtttctg attgccgaac gtctgcgtaa cctgggacgc tacacctctg
    19141 ccgatgtggc ctcttatcgt ctgaaacaag ggccgattcg tattctttcg gcctgtggtt
    19201 ctctggtagt ggtggcgctt taccttatcg ctcagatggt aggcgcaggt aaactgatcg
    19261 agctgctgtt tggccttaac tatcacattg cggtggtgct ggtcggcgtg ctgatgatga
    19321 tgtacgtcct gttcggcggc atgctggcga ccacctgggt acaaattatc aaagctgtgc
    19381 tgttgctgtt cggtgccagc tttatggcct ttatggtgat gaaacacgtc ggctttagct
    19441 tcaacaatct gttcagcgaa gcgatggcgg tacacccgaa aggtgtcgac atcatgaaac
    19501 cgggcggact ggtgaaagat ccgatctccg cgctctctct ggggctggga ctgatgtttg
    19561 gtacggcggg cttgccgcac attctgatgc gcttctttac agtcagcgat gcccgcgaag
    19621 cacgtaagag cgtgttctac gccaccggat ttatgggcta cttctatatt ctgaccttta
    19681 ttatcggctt cggcgcgatc atgcttgttg gtgcgaatcc ggaatataaa gacgcggcgg
    19741 gccatctgat tggtggtaac aacatggcgg ccgttcacct ggcgaatgca gtgggcggca
    19801 acctattcct cggttttatt tcagcggttg ctttcgccac tatcctcgcg gtggttgcgg
    19861 gtctgacgct ggcgggcgca tcggcggttt cgcatgactt gtacgctaac gtcttcaaaa
    19921 aaggcgcgac cgaacgtgaa gagctgcggg tatcaaaaat caccgtactg atcctcggcg
    19981 tgattgcgat tatcctcggc gtgctgtttg agaatcagaa catcgccttt atggtaggcc
    20041 ttgcgtttgc catcgcggcg agctgtaact tcccgatcat tctgctttcc atgtactggt
    20101 cgaaactgac tacccgtggc gcgatgctgg gcggctggct ggggctgatt accgcagtgg
    20161 tgctgatgat cctcggcccg actatttggg tacagatcct cggtcacgaa aaagccatct
    20221 tcccgtatga atacccggcg ctgttctcta tcagcgtggc attcctcggc atctggttct
    20281 tctcggcaac cgataactca gcggaagggg cgcgcgagcg tgaactgttc cgcgcgcagt
    20341 ttatccgctc ccagaccggc tttggcgttg agcaaggccg cgcacattaa tctctcccct
    20401 tccccggtcg cctgaccggg gaatacgctt cctctccaac atgcatcacc ttttcccata
    20461 atattaaaca aataaactca ttaaaaaatg agcgattttt gacagtcgta gaaaatgata
    20521 atgcagagaa tatgcctttt ctttcttgtt aattataagg atattttatg cgctataatg
    20581 gtttaaataa tatgtttttc cctctttgcc agattaacga taaccactct atcacaagtt
    20641 catcacatac aaagaaaaca aaatcagata attacagcaa acatcataaa aacacgttaa
    20701 ttgacaataa agccctctct cttttcaaaa tagatgatca tgaaaaagtg ataggcttga
    20761 ttcagaaaat gaaaagaatt tatgatagtt taccatcagg aaaaatcacg aaagaaacgg
    20821 acaggaaaat acataaatat tttatagata tagcgttata tgcaaataat aaatgtgacg
    20881 atagaattac gagaagagtt taccttagta aagaaaagga tgtatccatt aaggtggtat
    20941 attttataaa taatgtcgcc gtccataata atactatcga aattccacag atagtaaatg
    21001 gtggttacga tttttcacac cttagcctga agggtatcgt gattaaagat gaagatttat
    21061 ccaattcgaa ttttgcaggt tgcagactac aaaacgctat tttccaggac tgtaatatgt
    21121 ataaaacgaa tttttattac gccataatgg aaaaaatact ttttgataat tgtattctcg
    21181 atgactcaaa tttcgctcag ataaaaatgg ccgacggaac tctaaatgca tgctccgcta
    21241 tgcatgttca attctacaat gcagcaatga atagagccaa tattaaaaat accttccttg
    21301 actattcaaa tttttatatg gcgtacatgg ctgaggtaaa tctttataaa gtaatagcgc
    21361 catatgttaa tttatttaaa gccgacctta gtttctctaa actcgattta attaactttg
    21421 aacatgctga tctgtctcgc gtcaatctga acaaagcaat cctccagaat ataaacttaa
    21481 ttgatagcaa actcttttgt acgtggctga caaatacatt cctcgaaatg gttatatgta
    21541 ccggctctaa tatggctaat gttaatttta ataatgccaa tttaagcaac tgccatttca
    21601 actgttctat tttaacaaaa gcctgtatgt ttaatacccg tctctatcgg gttaatttcg
    21661 atgaggctag cgtccaggga atgggcattt ccattctccg tggggaggaa aatatcccca
    21721 ttgatagtga taccctggta acactacaga aattctttga agaagattgt acctctcata
    21781 ctggcatgtc acaaactgag gataatatta atgcagtcgc tatgaagatt actgcagata
    21841 ttatgcaaca cgcagattga ggttaagtcg aagaacagcc aatgaattga ctttacaaga
    21901 gagataactt atttgtttta gaatcatgaa tgttcgcccg agctaacctg ttaaaggtgc
    21961 aaaagattgc acctttttca gattacctcg ggcctggtta cgtcgctttc ggcttactga
    22021 ttttcctcaa ttagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt
    22081 tcgttgctga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat
    22141 gccgccagac ggaagcgacg ctcaaggttc tcttcctgca cggaactttc aacgtcgtta
    22201 cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactgacctc cgtaagcaac
    22261 tggttatcga tattctcttc ggtatccgcc gccagacgct cctccatttt ctggatcgcc
    22321 acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt cttctttcaa ttgttgcgaa
    22381 tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc
    22441 gagaagagaa tgacgccagc cgccaggaac accagctcat agcgcgccgg gaagacgtta
    22501 ccatccggca agagcagcgg gattgagagc acaccggcca gagtgatcgc cccacgcacc
    22561 ccagcgaaag acgcgatcag gatttctcgt gtggtccacg aaccaaactc catcggcttc
    22621 ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa acggaccagc
    22681 atcagcgccg catatatcag aataatatcg gtaaacagca tccagatttc gacgttaggg
    22741 tcgatttctg ccgccatcag cgacgtctcc agaatacccg gcagttgcag acctaacagc
    22801 aggaacacca tgccgttaaa gacaaattcc agcatcgccc aggtgctgtt tgcgcgcagg
    22861 cgcattgcca gtggcgcacg gcgcatcaca ccggagcggg tgatggtcat cccggcggca
    22921 accgcagcga ggatgcccga aacgccaata tgttcggcaa tcagataaga agcgaatggc
    22981 agcaagaaca gcagaacgat ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat
    23041 cgcagcgagc gaccatacag ccagctcacc acaaaaccgg caagaatacc gccaatggct
    23101 actttcataa attcgaccgt cgcaccgccg acagtgaaga tcatcgtccc catcgccact
    23161 gccacggcaa acttcaacga caccaggccg gaagcgtcat tcatcaacgc ttcgccctgc
    23221 aaaatgccca tgattttttt cgggatgcgc ccttcgccaa caatcccgga gagcgccaca
    23281 gcatcggtcg gagaaagtac cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc
    23341 accacccagt aaataaggaa gccaatgccg accacggtga ccaccaccag cgccagcgcg
    23401 aggccgaaaa tctctcgacc atgttcgaga aattcacggg tcggcgtttt ccagccatca
    23461 gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa ctccacatgc
    23521 aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag cggaagcggg
    23581 atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag ggtcattatc
    23641 agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat gatctgagaa
    23701 agacgttatg cctgaattct cattttttca gaatatcgca aaagctcaca caatgtgcgg
    23761 agaaaataaa aacgggcagc cattgccacc cgttttcaga atccgatgaa agattagata
    23821 gcccagccac ccgcatagaa ggtcaccagc gccacggcga taacgaccgt accgatgttc
    23881 aacttgcgcc attcaccgga aaccagacga ccaatcacca gagtcgcgaa gccgatcatg
    23941 atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag acccgccatc
    24001 gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc tcagcatcag caggccaacg
    24061 tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagaaagcgg agagaggaac
    24121 agaatcagca ggaacagcac gccaacggtg atagccgtca acccggtttt accgcccgcc
    24181 gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac caggccagag
    24241 aaaacgctgc tcatggagtc agtggtcagt gctttcccgc cgtcgatgat ctgcccatct
    24301 ttatccagca ggttcgcctg gccggcgaca gcgcggatag ttccggtggc atcaaatact
    24361 gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc gcccataatg
    24421 tccaggctgc caatcagtga attgccgttt tcatcgctca atgaaggcat ggcgaaaacg
    24481 ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc gataatggtc
    24541 agcagaatgc caccagggac tttcagtttt tccaagccga tgatcaccgc cagacctacc
    24601 agtgacataa tcaccgggaa ggtcgcgaaa tcacccagcg caacgggcag accatcaagc
    24661 gggtttttaa tcaccagacc gacaccatta gcggcaatga gcagcaggaa cagaccgata
    24721 ccgatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca gctacggata
    24781 cccgtggcag aaattaccgt aaacagcaca cccatcagga acacggcacc cagcgcgaca
    24841 ggtacgctaa tatgttgccc cagcaccagg ctgaatgcgg taaacgccgt cagggagatg
    24901 gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga accgagtccg
    24961 gcaaccagac aggttgcaac gaaaactgcc gcaggcggga agcctgcttt acccaacata
    25021 cctggaacga cgatgaccga gtagaccatc gccagaaacg ttgttaaccc ggcaaccact
    25081 tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg
    25141 gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt ttcgtcaaga
    25201 tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg tttgcggcaa
    25261 tttggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa
    25321 aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg ctgctttttt
    25381 ctcttttatt gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcgat
    25441 gtgcaagatt gcagcgttat cgagccgtat accgttctgg attgcgataa aaaatcgtct
    25501 gagatgacaa aaatattgcg gctaaaaagt atacttttac actggcgaag aaacgtcggt
    25561 gacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg aatgaggtgt
    25621 gttgacgtcg ggggaaaccc tcctgtgttc cagcgggata gagagaaaga caaagaccgg
    25681 aaaacaaact aaagcgccct tgtggcgctt tagttttgtg tttgcggata attccaaaag
    25741 agctcgatca gtcccctcgc cccttcgggg agagggttag ggtgagggga acaggcagca
    25801 ccagtgcaaa tatttaccct caccccggcc ccatagttaa cgatgagtca gcttgtcctt
    25861 gtggcgcttt agttttgttc atcttccagc aagcgtgcgc cggtaccttc ttctcctaag
    25921 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
    25981 ccgtccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa ctcttctcgc
    26041 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc tggcaacacg
    26101 ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcgat tttgataatt
    26161 gcgacatatc gcaacacatc acgtttatat cgccgctgat tgccgctgtt acggatacta
    26221 gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
    26281 gccacttcgc caggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
    26341 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
    26401 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
    26461 gacgtgcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc aggctattca
    26521 aagtggtact tgcaacggat gttccgcacg gtgacgcatc agacgcttgg cgattacatt
    26581 cgccaacgcc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
    26641 gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg
    26701 cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg cccgcgcgtt
    26761 aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg catcgccttc
    26821 gcaaaaaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc
    26881 tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg
    26941 tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tttcaacaaa cgatttatcg
    27001 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
    27061 aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag ccagaattaa tcggcgtcat
    27121 tttttcgaca tcaagaaatg catgctcagt cacttcaaat ttaatttgct gcggacgcac
    27181 cgcgtattgc tctgttttct gattgattcg cgctatcaac cgtgacgtat gaaaatcgga
    27241 ggccgacagg ttaatagaaa catagcgatc ggcatgtgtt gccaggtacg cgcccagatc
    27301 gcggaagaca ttatcaataa cataatcagt tatctgttct atcatcccct ccttttctgc
    27361 cagcggaata aactctgccg gattcattat ttgcccctgc tcaccaggcc aacgtaacaa
    27421 cgcttcagcg ccgatacatt tttctgtttt gatatcgatt attggctggt aataaagaca
    27481 aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat agttttgtcg
    27541 aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa
    27601 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
    27661 aattgggcgt tttgtcgaat aaattgtcgc atataaatag ccatttcttt gtacggttaa
    27721 atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa
    27781 aaaggttttc gtcaccgtat catacacacc ccattgcaat gtcgggtcat cagacatcac
    27841 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat
    27901 tttatagcca gagaaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg
    27961 cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa
    28021 aaaatggtta tcacgaattc attaatataa agatagccac gaataatatt caacattcgc
    28081 ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc gctcactaaa
    28141 tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa ggttcggagc
    28201 tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag aatgatggga
    28261 aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc
    28321 attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaac cctggctacc
    28381 gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg aaattgatac
    28441 ctttttaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa ttttctacag
    28501 gaatacagag gcggcgggaa gatatgccct taatgccact gaaaaaccac ataatttgta
    28561 cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat atagttcaca
    28621 aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg cgatagccag
    28681 agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct cttttcagag
    28741 gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc aacttcttag
    28801 tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt ctgattatgt
    28861 cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt tcgcaatggg
    28921 gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg agtcggtgat
    28981 gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct gatacgtaga
    29041 a
//
LOCUS       DAECOD010000045        28928 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.045, whole genome shotgun sequence.
ACCESSION   DAECOD010000045 DAECOD010000000
VERSION     DAECOD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.045"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KKO15_002022"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KKO15_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBD6360816.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KKO15_002023"
     CDS             complement(2138..2584)
                     /locus_tag="KKO15_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBD6360817.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KKO15_002024"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KKO15_002024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBD6360818.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLSDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KKO15_002025"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KKO15_002025"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBD6360819.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KKO15_002026"
     CDS             complement(5347..6009)
                     /locus_tag="KKO15_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBD6360820.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KKO15_002027"
     CDS             6182..6595
                     /locus_tag="KKO15_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBD6360821.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KKO15_002028"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KKO15_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBD6360822.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KKO15_002029"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KKO15_002029"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBD6360823.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KKO15_002030"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KKO15_002030"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBD6360824.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KKO15_002031"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KKO15_002031"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBD6360825.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KKO15_002032"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KKO15_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBD6360826.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KKO15_002033"
     tRNA            complement(9861..9948)
                     /locus_tag="KKO15_002033"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KKO15_002034"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KKO15_002034"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBD6360827.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KKO15_002035"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KKO15_002035"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBD6360828.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KKO15_002036"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KKO15_002036"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBD6360829.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KKO15_002037"
     CDS             14005..14592
                     /locus_tag="KKO15_002037"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBD6360830.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KKO15_002038"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KKO15_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBD6360831.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KKO15_002039"
     CDS             14984..15841
                     /locus_tag="KKO15_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBD6360832.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KKO15_002040"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KKO15_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBD6360833.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KKO15_002041"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KKO15_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD6360834.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KKO15_002042"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KKO15_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBD6360835.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KKO15_002043"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KKO15_002043"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBD6360836.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KKO15_002044"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KKO15_002044"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBD6360837.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KKO15_002045"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KKO15_002045"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBD6360838.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KKO15_002046"
     CDS             complement(22898..24037)
                     /locus_tag="KKO15_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBD6360839.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..26179)
                     /locus_tag="KKO15_002047"
     CDS             complement(24083..26179)
                     /locus_tag="KKO15_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD6360840.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QPYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26179..26925)
                     /locus_tag="KKO15_002048"
     CDS             complement(26179..26925)
                     /locus_tag="KKO15_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD6360841.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26922..27566)
                     /locus_tag="KKO15_002049"
     CDS             complement(26922..27566)
                     /locus_tag="KKO15_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD6360842.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27673..27978)
                     /locus_tag="KKO15_002050"
     CDS             complement(27673..27978)
                     /locus_tag="KKO15_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360843.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="KKO15_002051"
     CDS             complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="KKO15_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBD6360844.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7330 c         7607 g         6987 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgt cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtctagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt cagggcggta agttggtttg
    25261 ctaccgtggt gtactgcaaa ccttcagact ctggcgcagg ctgccaggcc ggttttggcg
    25321 tatcgcgtag ggcagggcgc agatcgttga aattggtccg taaagtgaaa ccaaacatca
    25381 acgtgtttcc gcgttcataa ctgaggttga ggtctgccca gctggcagcg cgataaactg
    25441 caccgacgtt gaaatggctt gcctgaggca gtttgccagc gaaatcattc tggtaattgt
    25501 tgccgtcgta ttcgagtttc agacgcaggg gattccacgg cgtttgatac tcaatgccgc
    25561 caaagatgga agccgggcca cgaaagatat cgctaaagct gatatcgccc gcatcgtgag
    25621 actctgcgcg atgacaatat ttatcgctca cccggcaaaa cgggttggta atattgcccg
    25681 cattaccggc gtagccccat gccatcccga gggtgaaatc aaatggcccc gcttgcttgc
    25741 tggccaccag atattcaccg tcaaacaaac ccgtaccagc aatatcacgt ttaccaaacg
    25801 ccacttgcgg tagccagtaa ccttcttccc acaggcgaag tttaaaatcg aatgatttgt
    25861 ctttgtatgg ctgatcgccg ctgaaatctt cccactggct atatttgcgt gtgcgcacat
    25921 ccgtataacg aatggtgcct tccagccatg ggaacagcgc cacggaggtg gagtagaacc
    25981 ggtattgatc gttatcccgg tagttgacgc tgaattcacc ttccggtgcg atgcgcgcat
    26041 ttggcatctg caacaatcct gtgccgccaa agtctgattg cgacgaaccc agcggatccg
    26101 ggtaggttaa tacttcagca tgacaggcac tggagacggc aatggccagg cagcttaaaa
    26161 gataagaatt cttcttcatc agtcaggaac acgttgcgtt aaaacagaca caatctggtc
    26221 atttaaatca gcatattttt cgggcagtac atgcgcagaa aagcccaacc aaagctggct
    26281 gccaggcggt ggttccacat gtcttttatt ccacagggca acaggggcga cgacagtctc
    26341 accctcaggc gtaatcacca taacgttatt cttgtctgcg cctgcaaggc gggggtgatc
    26401 ttgcagataa tcggtaacgc tacggcctgc ctgccagggc aattgcccag ccccagaaac
    26461 cgcacccagc agagtaatag tcaccggacg ctgtacggta tagagcgtat agtccccgac
    26521 caacggggga ttgctgttct catccactcg tacaaaatca gggtccagtt tgaccggtaa
    26581 gcgaccagtg atattgagat tgagtaactg ctggcgcacg gatttaatcg ttgccgccac
    26641 atcgtcgtca gcttctgctt cccaggaagc aagctgtgcc atgacatgtt ggtagtcttt
    26701 aagcgcttta gctttcgccg cagagtcggt cagcaaggca ccaggccacc agaggcgatc
    26761 gcgcagttgc ggttgtgtca ccaactggac aacattttcc acggggccaa cggaaagtgt
    26821 ctgttgttcg ctaggcagat aaatagtcac cgttccttgc gcaaaggcat ggggtgtcat
    26881 tacgtaaagt acgctggcaa tgaaatacga ctgtaattta ttcatggtgc gggtttaaga
    26941 aacgtcattt ctacgggaat aacccctgcg cctaacattt gccgactttg acgaacctgg
    27001 ccagtagccg aatcgaccca gaatgtgttt tgccactgtt tttctggcag cgtggaagtc
    27061 acctcttcct gccagacatt acacagcaca ttgtgacccg caatattcag cgtctctaat
    27121 ccagcaaagg aaaaagtaga acttagggtc gcagagcgaa aatggttgtc ttcactccag
    27181 agaatatcgc gcgtccagcg gctgccatct tttattgcca gcgcgttacg cagaggggcc
    27241 cgcccgggat ttgtcacttc cagcagatta ttattaagtt tgacggtctt taaaagacgt
    27301 ccattgtggg taaccagcat ggcgttatcc tgggataacc atttgctttg ttcttgttct
    27361 atatatccca gtaccacaaa gattcgttgc ccttcattaa ggcgtaaata catcgtggaa
    27421 taaggcaacg cctggatctg ctgagccgct acagtgatat cctgattatc gaaaaggctg
    27481 gcgcgaaatg tatcgaccat actttgctgg ctgtgcgtac atcccgcaag aaatagtgcg
    27541 aaaatcgata aaataagagg gcgcaccttt aatccttgaa aggttaatta aaaaaatgca
    27601 aatctctaaa gcccggtacg aatatcgcaa ccgggtatta tttaacttga atttcgacgc
    27661 gtttaagcag aattagcgcg tagtggacgt ggtggttgag gtgttatgag aaccgccatc
    27721 gccactgtca tttgctgcaa ctactgccaa cgtcgccaca ccaacagccg ttgccacacc
    27781 gacaactgtc gccgtgccga cagcgccagc agccacgcca ctagtggtag ccgcagtcgt
    27841 ggttgcacct gtagtggtcg ttgtggtgcc agtagtaccc gtcgttgcct cggtcccgtt
    27901 tgctgcttca ggggcagcaa acgcagctgg cgtcgtcaac atgaaggcca taaggattgc
    27961 agaaagcttg tgtttcataa cttttccttt attcatcgca tggacaatac gtacgcttga
    28021 gatcaggatt gtctactttg agtataacat ccttgtgaaa tctccctttc ctcataccgc
    28081 tagcactata tactgcgggt gggaaaggcg cgcagaggga aatataagat tgttaacgat
    28141 ttccctctgg aaatacgact tgtgagcata agataaatct tagaaaaata cgaattaaag
    28201 ataagaaaat tgttacgaaa gctattaatt attgttggta atatagtttc aagtggtact
    28261 ataactggct gttaaatggc atttataact attaggtgca gagatattcg cttaaatgga
    28321 ttagtttctt agtggaaaag taactgatgt tataatgtat ggtgttgact attgaactat
    28381 tccaataaaa ccagtcagct ttaaacaagc agcgtcatat taagagagat aaacatttgc
    28441 cgctgttggt cctcgcaggc catttacgcg gcaaaattcc acgcgtaatc ctggtataag
    28501 cacttctgcg tcgcggggag tgaatgcgga aatatggacc tggacttctt tacgaccgtc
    28561 ggaggggata atgaatcctt tgccgctttt gcgatcaaag gttttgacaa ttcctgtcat
    28621 tttacgggac aaacaaattc cttactgaaa atactgcgct gcactatacg gggttaataa
    28681 aataaagcca gcgatattta agaccgccgg acggctaaaa taaaatttgc ttaatctcaa
    28741 ttatcatgcc ttaatagctg cgtcggtttg aaagacagac agcatacaaa gtagtttact
    28801 aaagcagttc tcattatcag gcattatccc cttcttttga gtctctctcc tgaacactaa
    28861 gtggtttctg tattaaagcc ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg
    28921 tctaataa
//
LOCUS       DAECOD010000046        28863 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.046, whole genome shotgun sequence.
ACCESSION   DAECOD010000046 DAECOD010000000
VERSION     DAECOD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.046"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..653
                     /locus_tag="KKO15_002052"
                     /pseudo
     CDS             1..653
                     /locus_tag="KKO15_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            650..1036
                     /locus_tag="KKO15_002053"
     CDS             650..1036
                     /locus_tag="KKO15_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360845.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1061..3274
                     /gene="tssI"
                     /locus_tag="KKO15_002054"
     CDS             1061..3274
                     /gene="tssI"
                     /locus_tag="KKO15_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD6360846.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3297..3742
                     /locus_tag="KKO15_002055"
                     /pseudo
     CDS             3297..3742
                     /locus_tag="KKO15_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3764..4171
                     /locus_tag="KKO15_002056"
     CDS             3764..4171
                     /locus_tag="KKO15_002056"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBD6360847.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4168..6645
                     /locus_tag="KKO15_002057"
     CDS             4168..6645
                     /locus_tag="KKO15_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD6360848.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWSQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELVKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVALAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTPRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAVLPRRRTTA"
     gene            6642..7631
                     /locus_tag="KKO15_002058"
     CDS             6642..7631
                     /locus_tag="KKO15_002058"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360849.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7731..7934
                     /locus_tag="KKO15_002059"
     CDS             7731..7934
                     /locus_tag="KKO15_002059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360850.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            8036..8272
                     /locus_tag="KKO15_002060"
     CDS             8036..8272
                     /locus_tag="KKO15_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360851.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(8338..8570)
                     /locus_tag="KKO15_002061"
                     /pseudo
     CDS             complement(8338..8570)
                     /locus_tag="KKO15_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8680..9450)
                     /gene="yafV"
                     /locus_tag="KKO15_002062"
     CDS             complement(8680..9450)
                     /gene="yafV"
                     /locus_tag="KKO15_002062"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBD6360852.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9631..10077
                     /gene="ivy"
                     /locus_tag="KKO15_002063"
     CDS             9631..10077
                     /gene="ivy"
                     /locus_tag="KKO15_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBD6360853.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(10120..12564)
                     /gene="fadE"
                     /locus_tag="KKO15_002064"
     CDS             complement(10120..12564)
                     /gene="fadE"
                     /locus_tag="KKO15_002064"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBD6360854.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12804..13382
                     /gene="lpcA"
                     /locus_tag="KKO15_002065"
     CDS             12804..13382
                     /gene="lpcA"
                     /locus_tag="KKO15_002065"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBD6360855.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13588..14355
                     /locus_tag="KKO15_002066"
     CDS             13588..14355
                     /locus_tag="KKO15_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBD6360856.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14326..15066)
                     /locus_tag="KKO15_002067"
     CDS             complement(14326..15066)
                     /locus_tag="KKO15_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBD6360857.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15450..15971
                     /locus_tag="KKO15_002068"
                     /pseudo
     CDS             15450..15971
                     /locus_tag="KKO15_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            16147..16644
                     /gene="rayT"
                     /locus_tag="KKO15_002069"
     CDS             16147..16644
                     /gene="rayT"
                     /locus_tag="KKO15_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBD6360858.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16861..18573)
                     /locus_tag="KKO15_002070"
                     /pseudo
     CDS             complement(16861..18573)
                     /locus_tag="KKO15_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18545..19330
                     /gene="lafU"
                     /locus_tag="KKO15_002071"
     CDS             18545..19330
                     /gene="lafU"
                     /locus_tag="KKO15_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBD6360859.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19401..20456
                     /gene="dinB"
                     /locus_tag="KKO15_002072"
     CDS             19401..20456
                     /gene="dinB"
                     /locus_tag="KKO15_002072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBD6360860.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20508..20801
                     /gene="yafN"
                     /locus_tag="KKO15_002073"
     CDS             20508..20801
                     /gene="yafN"
                     /locus_tag="KKO15_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBD6360861.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20804..21202
                     /gene="yafO"
                     /locus_tag="KKO15_002074"
     CDS             20804..21202
                     /gene="yafO"
                     /locus_tag="KKO15_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBD6360862.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21212..21664
                     /locus_tag="KKO15_002075"
     CDS             21212..21664
                     /locus_tag="KKO15_002075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6360863.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLTKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21910..22953
                     /locus_tag="KKO15_002076"
     CDS             21910..22953
                     /locus_tag="KKO15_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD6360864.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            23015..23629
                     /locus_tag="KKO15_002077"
                     /pseudo
     CDS             23015..23629
                     /locus_tag="KKO15_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23686..25143)
                     /gene="pepD"
                     /locus_tag="KKO15_002078"
     CDS             complement(23686..25143)
                     /gene="pepD"
                     /locus_tag="KKO15_002078"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD6360865.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25405..25863
                     /gene="gpt"
                     /locus_tag="KKO15_002079"
     CDS             25405..25863
                     /gene="gpt"
                     /locus_tag="KKO15_002079"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD6360866.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25955..27199
                     /gene="frsA"
                     /locus_tag="KKO15_002080"
     CDS             25955..27199
                     /gene="frsA"
                     /locus_tag="KKO15_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD6360867.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27257..27658
                     /gene="crl"
                     /locus_tag="KKO15_002081"
     CDS             27257..27658
                     /gene="crl"
                     /locus_tag="KKO15_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD6360868.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27697..28752)
                     /gene="phoE"
                     /locus_tag="KKO15_002082"
     CDS             complement(27697..28752)
                     /gene="phoE"
                     /locus_tag="KKO15_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD6360869.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7130 a         7179 c         7677 g         6877 t
ORIGIN      
        1 aaaaaacaaa tattgatggc aatatcatta tacataagga tggtaggagg acgtatttat
       61 taaatcaaaa tactggttcg attacggctg tgataagatg gaagtatgag tagtataagg
      121 ataataccgt tacagatgac tggactatat gggaaaaagc tatttttatg cgagcgatat
      181 atttatctgt tcagcaggca tggaatggga aaataactta ttctgtttcc ggtgaatctg
      241 agttcgctaa aaaatttcag ggtaaagctt tgccttttga tgtaagaata atttctgcat
      301 ctcagaatga agactggttg gttattgcaa caaaggtttt gcctggtgct gatttaagaa
      361 catatgttga ttttaaaaac agtacggttc atgttgattc agcgggcctg gaaaaagtag
      421 cgaaatgtat taactgtaat aatacgttgc aagtaaatat acctcatgaa gccggacatg
      481 tacttggtta cttagatgat gattatgatt cttcgtcacc gtatgttgga gatattagtg
      541 ggttgatgaa tgtgggaatg gagcttcggg aacgatacct taaaaacgca actataacgt
      601 tgaatatcat aatgcctgaa actaaattta ctttattgaa tgtaaccaaa tgaaattttt
      661 ctgtattata gtgattttcc tttcaataaa ttacatagct atagtgaatg ttttcaagtc
      721 ttctgcagtt gctcaatcca ccaaatatta tgaagaaaat gatatacatg atggaagtaa
      781 tgacatcttt tttatgaaga aatatgggcc gcctgctcat gagttcgtga ggccacgata
      841 taaatttgtt ggtgagtatt atatcggctt gaatcaaact tatcgcataa cagatcctga
      901 atttcaagat gttcctatta aggaaatgtt ctggaatttg cagggggatt tgaatctgac
      961 atgttggttt cattataaag acgggcaatg gagagtgatt acttatattt tttggccacc
     1021 aggggctaga ttttaatccg gtattaaagg agcaaatatc atgagttttg tatctacggg
     1081 taataaacca gttggaaacg gtggacccgt catcaccaca ccaccaatag ctggcgaaag
     1141 tggcggtatg agcacaggtt cagctgtaac cgatgtatct ggtgccgccg aggagatggc
     1201 agaacaggct gcggcggacc tgtttggtgc gctgccggaa ccgtcaggtc tggttaaagc
     1261 cgcagtagct gctgcgcagg ctgccgctgc agcagcaggg atctcggata tggctggtgc
     1321 agttcaggat gcagcggcgt cgcttgcagc cggtgctccg ggggcgcata acgtcacagt
     1381 aagtggtagt gcggtgccgc cgcagatgct tttgtttgca ggcatgaatg gttcggaagg
     1441 gcttgggaat ctgttttctt acacagtaca gcttaaaacg ctggacgccc tgaacctggg
     1501 gtacgtttcc cccgctgcta acctgccgct tcagctcatg gtgggcaaag acctgtgcgt
     1561 cagtatcgaa ctggacggcg gcggaaaacg gtatatcagc gggctggtga cggcggcgag
     1621 ggtggtcggc catgagggcc gttcggtaac gtatgaatta cgcattgagc catggctgaa
     1681 gctgctgacc cataccagcg actacaaagc gttccagaac aaaaccgtgg tggatattct
     1741 cgatgaagta ctggatgaat attcctttcc ggtggaaaag cggctggtgg aaaattatcc
     1801 accccgcgcc tggcaggtgc agtatggtga aaccgacttc gatttcatcc agcgcctgat
     1861 gcaggaatgg gggatttact ggtggtttga gcacagcgag aacagccaca cgctggtgct
     1921 ggtggatgcc atcaatggtc ataaagcgtg ccctgactcg ccgctggtgg agtggcacca
     1981 ggaagggctg aaactggata aggaatttat ccacaccatt acggccagtg agaggctgcg
     2041 tactggaaag tgggtgatgg acgattttga ttttatgaag cctcgttcgc tgcttaaaag
     2101 taccgtggcc agtccgcgtg ataccggtca tgctgaatac gagcattacg aatggcctgg
     2161 tgattacttc actacgggcg aaggcgagat gctgacgcgc attcgtatgg aagcgcagcg
     2221 cagcccaggc agccgggcac atggggccgg tcatatccgt accctcatga ccgggtacac
     2281 ctttacgttg atgaatcacc cgacggcaga aattaatcag gaatatctgc tggtacagac
     2341 cactctgttt ttacgggata atgcgcagca cagcggtcag gatcagcatt ttacctatgt
     2401 caccacgttt gaactgcacc ccaccagcga agtttaccgc ccacaacgaa ccctgagcaa
     2461 accccacacc aaaggcccgc agagcgccat cgtcaccggc ccggcggggc aggaaatctg
     2521 gacggataaa tatggccggg tgaaggtgca gtttggctgg gaccgttacg gaaagaatga
     2581 cgaaaacagt tcctgctgga tccgcgtcag ctatccgtgg gcgggtaaag gattcggcat
     2641 gatccagatc ccgcgtattg gccaggaagt actggtggat ttcaaaaacg gcgatccgga
     2701 tttgcccatc atcgtggggc gcacctataa ccaggacacc atgccgccgt gggggctgcc
     2761 gggcatggcg tcgcagagtg ggatattcag ccactcattg cagggagggc cgactaacgg
     2821 aaatatgctg cgcttcgatg ataaaacggg tgcggaagag gtgaagttcc atgcggaaaa
     2881 agacctcaat accacggtaa aaaacaatga aacccacacg gtcatggtcg atcgcataaa
     2941 aaccattgtc aaaaatgaaa ccatcagcgt gggagaagac cgtaatgcca ccatcacaaa
     3001 aaatgatggt ctttccgtca aactggctca aaccatcaat gtcggaacga catatcgtct
     3061 ggatgttggc gatcaattta cccttcgttg cggcaatgca gcgcttgtat tacacaagga
     3121 tggctcgatt gagttttgcg gaaaacaact gttactgcac accagcgatg ttatgcaact
     3181 cattggcagt ggcattgata tgaatccgga tggcggcaca gccatgactg cggatgatat
     3241 tgccccgtta cctacccccg acgtttctga ataaaaaata taaaaggaat ttttctatga
     3301 ccagccttta ccgtattcag gagggctgct ttgccctgcc tgaaactttt cttgatcgca
     3361 ccgtcaatat cttcgtccca tcgggcaatg aaagggcaac tcccagtctg aatatttccc
     3421 gcgatacgct caggccagat gaaaatctga ccacctatat tgaccgccag atagccttga
     3481 tgaaaaaaaa aacctaggcc agcaccgggt actttcgcgg ggaacggcac tggcgggaac
     3541 tggcgaagct gccatgacag gtgaacagat tctcgcaact catcgttctg gaaaaacaga
     3601 agtgtaccag cgtcaggccg ggtttatcac cgggccagca aaggtactaa tgttgacgct
     3661 gaccactcag cgtccttttg atgatcacac cgaccagcta tggactgaat ggttgactag
     3721 ttttcagcct gctaaaagct aaaaaaaaca gggaggtttt gtcatgtatg aagccgcacg
     3781 ggtggatgac cccatctacc acaccagcgc ccttgccggg tttttgatag gggccatcat
     3841 aggtatcgcc attattgccg tcgccagctt tgcttttttt acctgcggct ttttggccgg
     3901 gctgattttg agttttctgg ctgatcagat agcttccgga gtcctgcaac tgggtgaggc
     3961 tattgggcgc tcgatacatt ccacagtggg aaaaattctg accggttctg ataatgtcag
     4021 caccaacagt cgcccggccg cccgggccgt actgagtacg gtgatatgcg aagaccattc
     4081 cccggaaatc cgcattgccc aaggttccgg caacatctac atcaacagcc agctgcccgt
     4141 aaggatgacc acactgagtg tgatgcagtg attgaggacg gctcccccaa tgtctttctc
     4201 ggcggcggta cgcagacggt actggaaatc agcccggaaa tccctgactg gctgcgtcag
     4261 gtggtggatg tgctgtatgt ggtcgccgga atgcttggcg ggctggctgg cgcctggagt
     4321 caggcggcaa agatggggtc gaaatttggt acaaaatgtg cggcaaaatt tattggtggc
     4381 gaaatggtcg ggatggccat ctctgacaca gtcatgggat tgttcagcaa tccggtagat
     4441 gtcaccactg ggcaaaaaat tttgctgccg gagacggatt ttaccctgcc aggacgcctg
     4501 cctgtcacct gttcgcgctt ttacgccagc cacctggaaa ctgaaggatt gctgggcaga
     4561 ggatggcggc tgaactggga gataaacctg cgtgaggatg aaacatatat caccttcatc
     4621 ggtgtacagg gtcgggaact gagttatccg aaagagatgc tgataccggg ccaccagata
     4681 ttcgacccgg aagagcagtt ttacctgagc cgtctgcatg acggatgcta tgtgctgcat
     4741 tacaccgacc gcagctatta cgtgtttgat gaatttgatg accacggcgt cgcgccgtta
     4801 ctttttatgg agacgccgta ccggcagcgt attgctttcg ggcgtgagaa cggcaggctg
     4861 gtgcgggtgg cctccagcag cggacaccat ctgttattgc accgcacgat gacgcaagca
     4921 ggggaacgcc tgtcgcatat tgaacttgtg aagggcgggc gtcccggtaa cctggtggaa
     4981 tatcgttacg acgataacgg gcaactgact ggcgtggtga accgggctgg tgtgacggtg
     5041 cgccagtttg cctatgaaaa tggcctgatg acagagcatc gcaacgcgac gggctttacc
     5101 tgtacctacc actgggaaga gattgaaggt ttcccgcgcg tggtggaaca cacgaccagc
     5161 gacggcgagg attaccggtt ccactatgat tttgctggcg ggcagacggt ggtaaccggc
     5221 aggccggagc agaagtggca gtggtggttt gatgaagaga cgtatgtgac tgcgcaccgg
     5281 acgccgggcg gcggaatgta ccgcttcacg tacaacgaaa accattttcc ggtagcggtg
     5341 gaactccccg gcgaacgcag ggtaacgctg gaatacgaca ccctgagccg ggtggtgaaa
     5401 gagacggacc cggcaggccg ggtgacacag acgcagtgga acggcagttt tgctgaaatc
     5461 acgcgccggg cgctggatga cgaccacgta tggaaggcgg attacaacga acacggccag
     5521 gtgatacggg agacggaccc ggaagggcgc gtcacgcggt acgggtatga tgaacagggg
     5581 ctggcggtca gccgcacgga tgcgcggggc ggcgaggttg cgctggcgca cgatgcgcgc
     5641 ggtcaactga ggcgttacac cgactgttca gggtgcgcca cgggttatga atacgatgaa
     5701 gacggcaacc tgacggcggt gacggacgcg gaggggaaaa cggtacgcat cagctataac
     5761 cgtcaggggc tgccggagac ggtctgccac cccggtaaac agcaggaccg ttacacctgg
     5821 aacgcgctgg ggttactgag cagccaccgt cgcatcacgg gcagtgtgca gtcctggcag
     5881 tatacgccgc gcgggatgct ggcgcgccat acggatgaag agaaacggga gacgcgctgg
     5941 cagtacaccc cggaagggct ggttgccagt ctgagcaacg gcaacggggc tcagtaccgg
     6001 ttcagctacg acggcgacgg acggctgacg ggagaacagc gcccggacgg cctgataagg
     6061 atgtttgcgc tgaatgcgga cgggtttccg gtcatcatcc cgacgcaggg cacagagggc
     6121 ggggtgcgca atgaacagca ggagcgtgat gcgctgggcc gcctgctgcg cagtgacacg
     6181 cagcacagta cacggacctt cagctacaac cgtcttgacc agataacgga agtgacgctc
     6241 acccccacgg aagaagggga acggctgcac cacatgcagg cggacacggt gcgttttgcg
     6301 tatgaccgca gcgggtggct gacggcagag cacagcgtgc acggcagtat caaataccgg
     6361 cgcgatgcgc tgggcaatcc caccgatatc acgttgccgg acgggcagca tctcagtcat
     6421 ctttactacg gcagcgggca tctgctccag acggcactgg acggtatcac ggtcagtgaa
     6481 tatgagcgtg acagcctgca ccgtcaggtg atacgcacgc aggggaaact ggcgacgttc
     6541 agcggctaca acgccgacaa ccggttaagc tggcagcgca gcctgccagg tggcagccag
     6601 aatccacagc aggcggtact cccccggcgc agaacgacag cgtgacggcg cgggactata
     6661 tctggaacgc agacggtgaa gtcggcggca tcaatgacaa actgcgcggt tgcctggtgt
     6721 tcagctatga ccgcagcggc tggctgacca gccgcacggg gcagatgtat gaccatgacc
     6781 attattatta cgacaaagcg ggcaacctgc tgacggatga gtatcaggga gcggtgatgg
     6841 ataaccgcct gccgggttac gggcgtgacc gctaccgcta taacgaatgg ggcgaactga
     6901 cagagcgcag ggaccagcag ctggagtgga acgcgcaggg ccagttaacg cgggtcatca
     6961 gcagcaacag cgagacgcgg tatcagtacg atgcgctggg caggcgcatc agcaaggcga
     7021 ccagcaatct ccataccgac cggggagaac gaagccggac aacgtttgtg tgggaagggt
     7081 tccggctgtt acaggagaca acgtggcagg gaaagcggac atacctgtac gatgcggaac
     7141 agccgtacac gccggtggct gccatcacgg ggcgcggaga gagccagaaa atctggtatt
     7201 accacacgga cctgacgggc acggtacacg aggtaaccgc gccggacggg acgctggtgt
     7261 gggcgggcta ccaggcggga tttggtgaaa accggggcga catcagcaac agcggggcgt
     7321 actttgagca gccgcagcgg ctgccggggc agtactttga cgaagaaaca gggctgcatt
     7381 acaatctgtt caggtattat gcgccggagt gcgggaggtt cgtaagccag gacccgatag
     7441 gattaaacgg tgggttgaac ttatatgctt atgcgcctaa tccgttaggt tggatcgatc
     7501 cgctaggact gaattcttta ggaatagatt caaataatat atttagagga gatggtaact
     7561 ataaaggtgg cggaattgga cagccaaaga gtggcacaat atcagcacag gacattattg
     7621 atcatctcta agaaaaaggt gatgggcgtt taacatcttt tagtactaaa aaaacatcta
     7681 gttccaactc tcgtggtgca gattttttct caaaaaacca tgtcttcaaa gtggcagttt
     7741 ctgatctcga tgatttagtt aaaaataatc agatcactgt tttaacacaa gaaaaagtaa
     7801 aagcaatatt tggtttagaa tcaagcaaga aaattagaaa agaacttggt aatattcttc
     7861 agaatatggc taggaataat gaggtgttag ttcatgggca aattcttgga aataaagtta
     7921 gccgatgtaa ttagttagtg gagaggaagt tgtggacgaa attagaataa gaattgctgg
     7981 taatacggct gcgccttgtt tccatgtgct aaaaaataag ggataaaaag taacaatgga
     8041 aactcatgca ttatctgacg attgtttaga ttgtgtatat gattggtatg cggaaaaaaa
     8101 tggacaattt tttagtgcaa cgagtccaga agagttactt ggattagttg caatgtggga
     8161 agttcgtggt aatgactgga gagctaattt gaatgagata aatacttatc aaaaagccat
     8221 agaagaatcc ccgttttttg ataaaaacgg caatgaaata gagtctgact aaattttatt
     8281 gatatagcaa cgataataac cggaaatttc ttccagatct ttccggcttt ttattattta
     8341 actttcgttc agcacactaa acatcccgtc tcgcatcccc ttaactggct gtttaacctg
     8401 ataaagttcc ctgcgcagtt cctgtacttc gttgttatcc caagcaggat caaacatccc
     8461 tccgagatct tcacggtaac gcccgtgccg atctcaaatc ccgcttttct cagccatttg
     8521 gcgctgattt tgatcaccgg cgtgtcgctg cggttgggca gataacccac ggtacagcga
     8581 cgctgtgttt tggaaatggt gacttctggc gtaatattgt gtttaagtta ggggctttcg
     8641 taatctagcc cagcccccgg ccctaaacac aacccccact caccacaacc taaactcatc
     8701 cgcatcccgc catgccggaa acttttctct atattcccgc aatgccacca tcgacagctc
     8761 cgcatcaata cgcgttgcct tatgcgcgtc ggcggtagcg ataatctcgc cttgcggatt
     8821 aatcacccgg ctgtcaccgc gataatggca gccgttgcca tcgctgccga cgcgattgca
     8881 tcccgccaca tacgcctgat tctcaatcgc gcgggccgtc agcaatgcct gccagtgcag
     8941 agagcgcgga gctggccagt tggcgacgta cagggcgagg tcataatcgt tgagattgcg
     9001 cgaccacact ggaaaacgta agtcgtagca caccagcggc aaaatacgcc agccgcgcca
     9061 ttccacaatc actcgcgcat tgcccgcttt ataatgcaga tgctcatctg ccatgcggaa
     9121 cagatgacgc ttatcataaa aatgtaccgt gccgcccggc tcaaccagca aaaagcggtt
     9181 aaccgaacca gactccgttt gtaatgcaac actgcctgca atcagcgcat tgcactgctg
     9241 cgccttagct gtcatccagt tcactacgtc atcttgtgca agcgacgaag ctgccgcttc
     9301 catggcaaag ccgctggtaa acatctccgg tagaacgatc acatcgcgcc cggtaatacc
     9361 ttccagttga cgatcaaaat ggcgcaggtt ggcaggacca tccacccaca ccaacggttg
     9421 ctgcaaaagc gtaatcttca aactaggcac ggtgtacaac tcctttatgc gaagggtttt
     9481 ataactttaa caccttatca ggcagttgcc ttagcgcaga ataaattgat aacaaatgct
     9541 gatattggaa atatctgatt tgcaaattat cgtgttatcg ccaggcttta ggaggttaat
     9601 aacatgggca ggataagctc gggaggaatg atgtttaagg caataacgac agtcgccgca
     9661 ctggtcatcg ccaccagtgc aatggcgcag gatgatttaa ccattagcag ccttgcaaag
     9721 ggcgaaacca ccaaagctgc gtttaatcag atggtgcaag ggcataagct acctgcatgg
     9781 gtgatgaaag gcggtactta tactcccgca caaaccgtaa cgttgggaga tgagacgtat
     9841 caggtgatga gcgcgtgcaa accgcatgac tgtggctcgc aacgtatcgc tgtgatgtgg
     9901 tccgagaaat ctaatcagat gacggggctg ttctcggcta ttgatgagaa aacgtcgcaa
     9961 gagaaactca cctggctgaa tgtgaacgat gcgctttcga ttgatggtaa aacggtgctg
    10021 ttcgcggcgt tgaccggcag cctggaaaac catccggatg gctttaattt taaataatta
    10081 gcggataaag aaacggagcc tttcggctcc gttattcaat tacgcggctt caactttccg
    10141 cactttctcc ggcaacttta ccggcttcgt cgccagctct tccggatcaa agtcatcaac
    10201 gttaatactg cgcagacggc tttcttccgc tttcaccaga atagcggctt catctttatc
    10261 aatcagcccc ttcgtcagcg cgttgtgcgc cagttcatcc agacgggtaa atggcagatt
    10321 tttacccagc tctttacaga tccgctgatg aatcgggtcg gcggcaatca catccaccaa
    10381 cgcctcttcc agtaaaccaa ccgggttatg ctcgcttggc gtcaggtact gaccgcgacc
    10441 aatacgtgaa cgagtggcgt tcggcacttg taaaatcttc gccactttat gatccagctt
    10501 gtcagacggt gccagataat gacgtccggt cgggaagatc accacattca gcagcccggc
    10561 aaccacgcgg ttcgggaagt tttgcagtaa attgtccatt gcctgttctg cctgatacag
    10621 cgcatcctgt acgccccagt gcaccagcgg cagatcggct tcattacggc cttcgtcgtc
    10681 ataacgcttc agaacggcag aggcgaggta aagctggctt aaaatatccc ccagacgtgc
    10741 cgagatacgc tcacgacgtt tcaggctgcc gcccagcagt gccatcgaga catcagaaag
    10801 cagggcgagg ttggcgctca ggcggttcag gtgctgatag tagcgtttag tggcatcgcc
    10861 ggttggcgtg ctgctggtta aaccgcgcgt caggcccagc cagaagctgc gaactttgtt
    10921 gctaccgacg tgaccgatat gtttgaacaa cagtttatcg aacgcgttga cgtcattgtt
    10981 cttcgccgct tccatctctt ccagcacgta cggatggcaa cgaatcgctc cttgtccgaa
    11041 gatcatcatg ctgcgggtca gaatgttagc cccttcaacg gtgatggcaa tcggtgcgcc
    11101 ctggtaagca cgcgccagga agttgctttg cccgagcata atgcctttac cgccggtaat
    11161 atccatcgca tcaataatcg actgctgccc gcggtgggta cagtgatact taacgatagc
    11221 cgacagcacg gcaggttttt cgccgagcat aatgccgtag gtaatcagcg atgccgcagc
    11281 atccattaca taagcgttcc cggcaatacg cgccagcggc tcttcaatcc cttccatctt
    11341 accgatagag attttgaact gacggcgaat gtgagaatac gcgcccgttg ccagcgctac
    11401 cgatttcacg ccgccggttg agttggaagg cagggtgatg ccgcggccta ccgagaggca
    11461 ctccaccagc atccgccagc cttgcccggc cattttcggc ccgccgatga tgtaatcgat
    11521 cggcacgaag acatctttac cgcgcgtcgg tccgttctgg aacggtacgt tcagcgggaa
    11581 gtggcgacga ccaatttcca cgcccggcgt ggtggttggg atcagcgcac aggtaatgcc
    11641 taaatcttct gcaccgccga gtaatttttc cgggtcggag agtttaaacg ccagcccaag
    11701 cacggtcgca atcggtgcca gcgtaatgta gcgtttgttc caggtcagac gcatccccag
    11761 cacctgctgg ccctgccatt cgcccatgca gacaatcccg gtgtccggaa tcgcgcccgc
    11821 atcggaaccc gcttccgggc tggtcagtgc aaagcagggg atctcctgac cacgcgccag
    11881 acgcggcaga tagtgatctt tctgctcgtc agtgccgtaa tgttgcaaca gttcgcccgg
    11941 gcctaatgag tttggcacgc cgacggtaat cgccaggatc ccgctcacgc cagagagttt
    12001 ttgcagcaca cgagactggg cataagctga gaactccagt ccgccgtact cttttttgat
    12061 aatcatcgcg aagaagcgat gctctttaag gtacgcccac aactccggcg gcagatccgc
    12121 cagctcatga gtaatctgga aatcattcgc catccggcag gcttcttcta ccgggccgtc
    12181 gagaaacgct tgctcttcag cggtcaggcg cggctgcgga tagttatgca gctttttcca
    12241 gtccggcttg ccctggaaca agtcgccctc ccaccaggtg gtgcccgcat caatcgcttc
    12301 tttctcagtg cgcgacatcg gcggcatcac cttacggaaa ccgcgaaata ccggcgcgga
    12361 aatcatcgac ttacgcatag gcgcaaagtt aaatggcacg aggataatgg tcagaggcac
    12421 cagtacccac gccgaccaca gaccagcaac gccgagggcg gctgtccagg cgagcaaaat
    12481 cagactgctg ataaataagc tcacgcggtg atagaacaac gcgccgagca ggacaaccgt
    12541 agcgagaata ctcaaaatca tcataacgaa aagcccctta cttgtaggag gtctgaccac
    12601 ttgtgatgat atggttgtag tggatgtaaa aacatttagc aatgtgttta caatataatt
    12661 acaacaaagc tcacattgtt gctgttttta tccgcacttc aggtcaaaaa gtcctggtca
    12721 tagcacctgc ccgtacttct cgcttttggc ggtatccggt acactgcatt ttgtctatta
    12781 catttatgct gaaggatatc ctcatgtacc aggatcttat tcgtaacgaa ctgaacgaag
    12841 cggcggaaac gctggctaac tttttaaaag atgacgccaa tattcacgcc attcagcgcg
    12901 cggcggtcct gttagcagac agctttaaag ccggtggcaa agtgctttcc tgcggcaacg
    12961 gcggttccca ttgcgacgct atgcactttg ccgaagagtt gaccggtcgc taccgtgaaa
    13021 accgtccggg ctacccggcg attgctattt ctgacgttag tcatatttcc tgcgtcggta
    13081 atgatttcgg tttcaatgat attttctccc gctacgttga agcggtaggt cgcgaaggcg
    13141 atgtactgct ggggatctcc acctccggta actctgcaaa cgtgatcaaa gcgatcgcag
    13201 cggcgcgtga gaaggggatg aaagtgatca ccctgaccgg taaagacggc ggtaaaatgg
    13261 ctggcacggc ggatatcgaa attcgcgttc cgcactttgg ttatgccgac cgcattcagg
    13321 agattcacat taaagtgatc catatcctga tccagttaat tgaaaaagag atggttaagt
    13381 aagtctggcg taggccggat aaggcgttta cgccgcatcc ggcacttgtg cgctgatgcc
    13441 tgatgcgatg ctgacgcgtc ttatcatgcc tacaaatctg tacccgaacc gtaggccgaa
    13501 taattcgttt acgccgcatc cgacctgaaa attcttaaat caatcttcgc cggggtccat
    13561 gcgctcccgc tgttgtggag gttacccatg tgcgaattgc tcgggatgag cgccaacgtc
    13621 cctaccgata tctgctttag tttcaccgga cttgtacagc gtggtggtgg aaccgggcca
    13681 cataaagatg gctggggcat taccttttac gaaggtaaag gctgtcgcac atttaaagat
    13741 ccacaaccca gctttaattc ccccatcgcc aaacttgtcc aggactaccc gataaaatcc
    13801 tgttcggtgg tggctcatat tcgccaggct aatcggggcg aggtggcgct ggaaaatact
    13861 cacccattta cccgcgagtt atgggggcgt aactggactt atgcccataa cggacaactg
    13921 acgggctaca aatcactgga aaccggcaac ttccgcccgg tcggtgaaac cgacagcgaa
    13981 aaagcctttt gctggctgct gcataaatta acgcagcgtt acccgcgcac gccgggcaac
    14041 atggcggcag tgtttaaata tatcgcctca ctggcggatg aactgcgggc gaagggcgtt
    14101 ttcaacatgc tgctttcgga cgggcgctat gtaatggcgt attgctcgac taatttacac
    14161 tggatcaccc gccgcgcgcc gtttggcgtg gcaacgttgc tggatcagga tgtggaaatc
    14221 gacttcagct cgcagaccac accgaatgat gtggtcacgg tgattgcgac acagccgctg
    14281 acgggcaatg aaacctggca aaaaattatg ccaggcgaat ggcgcttatt ttgcctcggg
    14341 gagcgtgtag tttgatgcca gttgtggctg caccacttcg tggcttaacg gcttgctgac
    14401 cacgtaacgg ccattggcca cagaaacggt cggtggctta cgggtttgct caaagtagtc
    14461 gtagcccggc ttcagctgct cccagaagtc cttaaagttg gaatatttat ggcgcttcat
    14521 attggcgtcg gtcatgcgga atgggtaaat actcacctgc acgctcggct gaccaaacac
    14581 cagcgcacca gtaacgaact ggaagatctc atcaataccc tgattggtca ttgcgtagca
    14641 gccgatggat acacaatcgc cgtggatcat caggtatttc ccttcataac catgcgcacg
    14701 gtcataagca ttggggaatc caatattaat cgctttgtag taacggctgt ctggttttaa
    14761 ctgattacgc tggacgctat aaaacccttc cgggcttttg aaatcgccct gacgctgttt
    14821 tggccctaag ccgccggaat atttacagat tttataactg tcgagcagtt gatattgctc
    14881 gcccattttg acgtaaagat cgagcgtacg ttcttccttg aagatctgga tgtagacagg
    14941 ggatcccatc aactgctgct tatactcttt gctcactggc gtggtcgagc tactgctgcc
    15001 cagcaaaccg gcaaacgaaa cgcacgggat caacaacatc gcaagaatta atgcgatttt
    15061 acgcatactg cttattcctt gataaaacgg ttacacacgc caggacggca aaatggatcc
    15121 caaatcggaa tagtctggat ttggaaggct cacattatca ccaaaagagt tttacgcaag
    15181 cctgtcggcg ctgggtttac aaatttagca caggggcagt tttcaatagt ccgaaaagcg
    15241 catgtatcat ttatttccta atattatgaa atctcaatag taatatgcaa ataagcaatt
    15301 aataatcccg tttactcctc cggtctttta aaattctcac cttcattcag ccggtttatg
    15361 tctttcatgt cttcgttctt gctcggcaga tttttgcatc caggcgtgtt ttcgttatgc
    15421 gttttgctcc ctttactcgc cagcgccacc acaaccggga gcagataaaa cagcatccag
    15481 aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg gcggaaaaca
    15541 accacttttt aagcagcgat cacctgcata acatcaccga agtggcgatt caccgcctgg
    15601 agcagcagct tggcaagcct tacgtctggg gcggcacgcg gcccgatcaa ggcttcgatt
    15661 gtagtgggtt agttttttat gcctacaaca agatccttga ggcgaaactc ccgcgcacgg
    15721 ccaatgagat gtaccactac cgtcgggcaa cgattgtggc gaataacgac ctgcgccggg
    15781 gagatttgct atttttccat atccacagcc gcgagattgc cgaccatatg ggggtgtatc
    15841 tgggcgacgg tcagtttatt gagtcactgc gcaccgggga aaccattcgg gtaagccggt
    15901 tagccgaacc tttctggcag gaccattttt taggcgcgcg caggattttg acggaagaga
    15961 tgattttgta ggccggataa ggcgtttacg ccgcatccgg cggttgtgca cggatgcctg
    16021 atgcgacgct gacgcgtctt atcatgccta caaatctgtg cgtttttgcg agccgctttc
    16081 ccgatataac acagcgtcat tgtcggcctt tccttttcct cttaccgttg agaaaaagga
    16141 gtcgctatgt cggaatatcg tcgttattac atcaaggggg gaacatggtt tttcacggta
    16201 aatttacgca atcgtcgaag ccatcttctg accacccagt tccagacgct ccgtaacgcc
    16261 attattaacg ttaagcgaga cagacctttt gaaattaacg cctgggtcgt tttgccggag
    16321 catatgcatt gcatatggac attacccgaa agcgatgatg atttttcctc tcgctggcgg
    16381 gagattaaaa agcagttcac ccatgcttgc ggattgaaaa atatctggca accgcgtttt
    16441 tgggagcatg ccattcgcaa tacaaaagat tatcgccatc atgttgatta tatttatata
    16501 aatccagaga aacatggctg ggtaaagcag gtgagtgatt ggccattctc aacgttccat
    16561 cgcgatgtcg cgcgagggtt atatcccatc gattgggcgg gagacataac ggatttaagt
    16621 gccggggagc gtatcatttt ataactgtgc gctggtgccg gatgcgctta cgcttatccg
    16681 gcctacaaat cgtgctttaa cggtaggccg gataaggcgt tcacgccgca tccggcagtt
    16741 atgcgcagat gcctgatgcg acgctggcgc gtcttatcat gcctaaaaac ttgtgcctga
    16801 acggtaggcc ggataaggcg tttacgccgc atccggcaat tgtgcaccga tgccggattc
    16861 ttacatcaac gcccccataa tcttcaactc cagctcatcc ggcacttcgt tatacgacag
    16921 cacatgcagc cccggcgcaa acaaccttgc ataacgcgcc agcaaagggc gtagctgcgg
    16981 cggcaccagc agcaccggat ctttccccgc cgctttcatc tgctctttca cctgtggcat
    17041 cgtgctctgg aactggttga gcatattcgg gtcaaccggc acgctgtcga gcatcacttt
    17101 gccggcctgt tgcgcctgat tcactacgtt ggtcagcaga ttttccagct cattattcag
    17161 cgtatacacc gtcagctcct gcttgcgaac gaacggatga gtaatgctgc gccgcagcgc
    17221 caggcgcaca tcggccgcca gcagaatatg atctttagtc accgtgctac tggcgaccag
    17281 cacggtggcg atggtgacga tatcgcgcag ggaaacgcct tcggtcagca gcgcccggta
    17341 cactttcagc aattggctgt aattgagcgc cgcgcttaaa tcctccgcca ggcgcggtgc
    17401 catcgacgac aaacggttat gcaactgcgt gatgtcatca tagttaaaca aatcaggaat
    17461 atagctgcgc acaatcttgt tcacatgggt ggcaatcaca ctggcgctgt cgatcacctg
    17521 ataccccata ttcagagcct tcgctttctg cgcaggctgg atccaggtga ccggtatccc
    17581 gtacgccggg tcgttcccca gcacgccgtc aatctcgccg taggtttcgc tggagggcag
    17641 cgccatcagt ttatccgccg gaatatccgc ttcatcagcc tcaatgccgt tgatgaaaat
    17701 agcgtactgg ctgggcttga ggcggaagtt ttcccgaatg cggatctccg gcagcagcac
    17761 gccgttaccg tcggaaatca cctgccgaac gccgcgaatc cgctgggtga gcgggttgcc
    17821 ctgcgctttg tccaccagcg ccaccagctt gtagccgagg cttaagctga tgggctcgat
    17881 cagcggaata gtttcccaac tgacctgttg ttcgctggtt tcagtgatgg tgcgggtcag
    17941 cgtttcgagg cttttctctt ccgcctccgc cgcctgcggg cgtttgctca tccgccagcc
    18001 ggtaaagcca agcagggcgc tgaacagcaa aaacggcaga tgcggcatcc ccggcaccac
    18061 cgccagcacg aacatgatcc cggtggcggt gtagagcacc gacgggcttg ccagcaactg
    18121 gtggcgcacg tcatgggcga tatcgccgtt gtcgctgacg cgggtgacga taatcgccgc
    18181 cgcggtggag agcagcaggg aagggatctg cgccaccagg ccgtcgccga tggtcatcag
    18241 cacgtattgc tggaaggcgg cgtcggcgct caggttgtat ttgaagatgc cgatacagac
    18301 gccgccgctc aggttgatcg ccagaatcat catcccggcg atggcgtctc cgcgtacaaa
    18361 cttcgatgcc ccgtccatcg cgccgtagaa atcggcctcg ctggcaacat ctttacgccg
    18421 ggtttgcgcc tgcgcctggt tgatcaatcc ggcgttaaga tcggcgtcaa tcgccatctg
    18481 tttgccgggc atcgcgtcca gggtaaagcg ggcagaaacc tcggaaatac gctcggcctc
    18541 tttggtgacg acgataaagt tgattgtcaa cagcgtcagt aaatccgagc gtgaaagcat
    18601 tatcgccgcg ctgcacgggc agtcgatttt taacggtggt gggctgtcgc cgttaaataa
    18661 aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag agacggagaa
    18721 aaaagcgcgt gatgtgaatg agaaaacggc gctgctgaag aagaaatccg ccaccgagct
    18781 tggcgagttg gcaaccagta tcaacaccat tgcccgtgat gcgcatatgg aagcgaatct
    18841 ggagatggag attgttcccc agggattacg cgtgctgatt aaagacgacc agaatcgcaa
    18901 tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaactttgc tggtggagct
    18961 cgcgccagtg ttcgactcgc tctataacaa aattattatt accgggcata ccgatgcgat
    19021 ggcctacaaa aacaatatct acaacaactg gaacctttcg ggcgatcgcg cgctttcagc
    19081 tcgtcgggtg ctggaagagg ccggaatgcc ggaaaataaa gtgatgcagg taagcgcaat
    19141 ggcggaccag atgctgctgg acgcgaagaa tccgcaaagc gcgggcaatc ggcgtattga
    19201 gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg
    19261 ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg
    19321 aatgcgctaa atgctgaatc tttacgcatt tctcaaaacc tgaaatcact gtatacttta
    19381 ccagtattga gaggtgagca atgcgtaaaa tcattcatgt ggatatggac tgctttttcg
    19441 cagcggtgga gatgcgcgac aatcccgctc tgcgcgatat ccctattgct attggcggca
    19501 gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac
    19561 gtagcgctat gcctactggg atggcgctca aattatgccc acatctcacc ttgcttccgg
    19621 ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca
    19681 cctcgcgcat tgaaccgttg tcactggatg aggcttatct cgatgtcacc gatagcgtcc
    19741 attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatc ttcaacgagc
    19801 tgcaactgac ggcatctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg
    19861 acatgaataa acccaacggt cagtttgtga ttacgccggc agaagttccg gcatttttac
    19921 aaaccttacc actggaaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag
    19981 cgatggggct gcggacctgc ggtgatgtac aaaagtgtga tctggtgatt ctgcttaaac
    20041 gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg
    20101 ttaacagcga acggttacga aaatccgtcg gcgtggaacg cacgatggcg gaagatattc
    20161 atcactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc
    20221 tggcaaaggt aaaacctgat ttactgattg ctcgccaggg ggtgaaatta aagttcgacg
    20281 attttcagca gaccacccag gagcacgtct ggccgcggct gaataaagct gacttaatcg
    20341 ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcgt ctggtggggc
    20401 tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgatgta
    20461 tactattatg tatattctgg tgtgcattat tatgagggta tcactgtatg catcgaattc
    20521 tcgctgaaaa atcggtcaat atcactgagt tacgtaaaaa cccagctaaa tactttattg
    20581 atcaaccagt tgcggttctt tctaataatc gccccgcagg atatctctta agtgccagcg
    20641 cattcgaagc gttaatggac atgcttgctg aacaagagga gaaaaagccc ataaaggcgc
    20701 gctttcgtcc aagtgctgca agattagagg aaattacacg ccgcgctaaa caatatctta
    20761 atgatatgac ggatgatgat ttcaatgact ttaaggaata aggatgcggg tattcaaaac
    20821 aaaacttatt cgcctgcaac ttacagcaga ggaacttgat gcgttaacgg cggattttat
    20881 ttcctataag cgtgacggtg ttttgccaga tatatttggt cgcgatgcac tctacgacga
    20941 ctcgtttacc tggccattaa tcaaatttga gcgagttgct catattcatc tggcaaatgt
    21001 gaataatcca tttccgccac agttgcgcca attcagcaga acgaatgacg aagcgcattt
    21061 ggtatattgt cagggtgcgt ttgatgagca agcatggttg ctcattgcca ttctgaagcc
    21121 tgaacctcat aaactggctc gagataacaa ccaaatgcat aaaattggaa aaatggcaga
    21181 agcgtttcgc atgcgttttt gaatttatat tatgaataac atacaaataa gagattatca
    21241 gcctggcgat tttcagcaac tatgcgctat tttcattaga gcggttacga tgaccgtcag
    21301 tcagcattat tcactacaac aaattgccgc ctgggcgcag attgacgaat ctcgctggaa
    21361 ggagaaactc acgaaatcac aagtgcgggt tgcagttatt aatgcaaaac tggtcggctt
    21421 tattacctgc gttgaacact atatcgatat gttatttgtt gaccctgaat acacccgccg
    21481 tggggttgcc agcgctttgt taaaaccttt tattaagtct gaatccgaac ttacggtgga
    21541 cgcaagcata accgcaaaac ccttttttga acgttatggt tttcagacag ttaagcagca
    21601 gcgcgttgaa tgccggggag cgtggtttat taatttttat atgcgttata aaccgcaaca
    21661 ttaaatccag cttgcaatga aaataacgcc cgcctagtat gtgcgcgttt tcttattcca
    21721 caaactgcaa ggaggtaaat catgacaaat cctttatcaa tgactctttg cagacctttc
    21781 caggattaat tctttttttc ttgccctgga ttcgtctgcc atttcctgat tttttaattg
    21841 ttactctcaa taattcaagt cgcagcacat gcaacttgaa gtatgacgag tctatttacg
    21901 gaatggtata tgggcaatta tattcgtccc ttatccgatg cggtatttac catcgcatct
    21961 gatgacctgt ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc
    22021 aacatgcagc gcgtagtggg gatgccagat ttacaccccg gacgcggcta cccgattggc
    22081 gcagcgttct tctccgcagg ccgtttttac ccagcactgg tcggcaatga tatcggctgt
    22141 ggcatggcgc tatggcaaac agatattctc gctcgcaaat acaacgccga taagtttgaa
    22201 aagcgattat cagctttgga tgacgttgct gaagaaagct ggctggagga aaacctgccg
    22261 tcagcatttg cacagcatcc gtggcgcagc tcgcttggct ctatcggtgg tggcaatcat
    22321 ttcgctgaac tgcaacaagt tgatcaaatc atcaacgctg aactgtttgc actggcaggt
    22381 ctggatgcgc agcatctgca actgctggtt catagcggct cgagggggct agggcagtct
    22441 attttacagc ggcatattgc ctcgttttcg catcatggtt tgcctgaagg cagtgacgac
    22501 gcgctggcgt ttgcgcgttt taatcgccac ctgatcgctt tgcgcataat gcaacaggtt
    22561 aaggccaccg gaagtccggt tctggatgtg gcgcataact ttgttagcgc gtgtcgaatc
    22621 ggtgatcaac aaggggtctt acatcgtaaa ggtgccacgc ctgatgactg tggtctggtg
    22681 gttattcccg gttcacgcgg tgattactcc aggctggttc agcccgtcag gagtgaggaa
    22741 acattgcatt cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg
    22801 cgtctggcag cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta
    22861 atttgtcgcg ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa
    22921 agtgtggtgc aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg
    22981 tgctaacgct caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc
    23041 gcaggggccg gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga
    23101 agctgcccga caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga
    23161 cacgctgcgt tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag
    23221 ctggtgtggc actattcagt ggatttgccc gagcccgtat cgcccacatc acgggcgtaa
    23281 aaactggttt ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat
    23341 ccggtatgag acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc
    23401 ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag
    23461 ccagcatgct aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca
    23521 ataggaaaat agcgcggtgc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg
    23581 tggcaacccg cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa
    23641 actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat
    23701 ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg
    23761 ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg
    23821 atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt
    23881 cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc
    23941 cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag
    24001 cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca
    24061 gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac
    24121 cacacctttt gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg
    24181 aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag
    24241 caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt
    24301 attcaccaga gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc
    24361 ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag
    24421 ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac
    24481 gtggatttca ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc
    24541 aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc
    24601 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
    24661 ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt
    24721 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga
    24781 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc
    24841 atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg
    24901 cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt
    24961 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
    25021 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
    25081 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
    25141 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
    25201 aaaatgttgt caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc
    25261 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
    25321 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
    25381 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
    25441 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
    25501 attgccgtaa gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt
    25561 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    25621 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    25681 accggtggta ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    25741 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    25801 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    25861 taatcttttc aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca
    25921 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    25981 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    26041 cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg
    26101 catgattaac cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt
    26161 ccaggcacgt attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt
    26221 gattggctac cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca
    26281 acaaaaagcc tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc
    26341 tacgttgtac aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc
    26401 gcaggcttta tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg
    26461 gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa
    26521 aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga
    26581 ctattacagc ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga
    26641 tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca
    26701 tcagcacgtc ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc
    26761 ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg gcataccttg aatcgccgcg
    26821 tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg
    26881 ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga
    26941 tgcttccgac gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt
    27001 gctggggcgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag
    27061 cccggaagag gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat
    27121 cccatttaac ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat
    27181 cgaaaaacgc ttgtgttaaa aatttgctaa attttgctaa tttggtaaaa cagttgcatc
    27241 acaacaggag atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa
    27301 atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt
    27361 cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg
    27421 ctggtggatg gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct
    27481 gttcgataaa gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg
    27541 actggagcac accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa
    27601 tctgaagctg gaaccggcga atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt
    27661 gaaatgtgcc ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac
    27721 cgcgacagta tcatcattat taatattcag cttattatcg ctatccagtt ggttgatttt
    27781 ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata
    27841 attgaccaga tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc
    27901 cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt
    27961 cttattggca aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc
    28021 cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc
    28081 tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact
    28141 aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt
    28201 ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc
    28261 atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt
    28321 cataaagttg tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca
    28381 ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt
    28441 atatttcaac ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt
    28501 atttccggca aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt
    28561 ttcaccttta aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact
    28621 catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata
    28681 tatttctgca gcctgtacag atgcagatgc cacaatgccc atcaccacta atgccagagt
    28741 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc
    28801 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat
    28861 att
//
LOCUS       DAECOD010000047        28403 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.047, whole genome shotgun sequence.
ACCESSION   DAECOD010000047 DAECOD010000000
VERSION     DAECOD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.047"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="KKO15_002083"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="KKO15_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBD6360870.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="KKO15_002084"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="KKO15_002084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBD6360871.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="KKO15_002085"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="KKO15_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBD6360872.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="KKO15_002086"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="KKO15_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBD6360873.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="KKO15_002087"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="KKO15_002087"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD6360874.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="KKO15_002088"
     CDS             6426..7055
                     /locus_tag="KKO15_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBD6360875.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="KKO15_002089"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="KKO15_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBD6360876.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="KKO15_002090"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="KKO15_002090"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBD6360877.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="KKO15_002091"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="KKO15_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBD6360878.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="KKO15_002092"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="KKO15_002092"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBD6360879.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="KKO15_002093"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="KKO15_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBD6360880.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16314
                     /gene="adiC"
                     /locus_tag="KKO15_002094"
     CDS             14953..16314
                     /gene="adiC"
                     /locus_tag="KKO15_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBD6360881.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKDKFPLI
                     QQT"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="KKO15_002095"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="KKO15_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBD6360882.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="KKO15_002096"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="KKO15_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBD6360883.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="KKO15_002097"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="KKO15_002097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBD6360884.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="KKO15_002098"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="KKO15_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBD6360885.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="KKO15_002099"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="KKO15_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBD6360886.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="KKO15_002100"
     CDS             complement(21746..22624)
                     /locus_tag="KKO15_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBD6360887.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="KKO15_002101"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="KKO15_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBD6360888.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25251..25586
                     /locus_tag="KKO15_002102"
     CDS             25251..25586
                     /locus_tag="KKO15_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBD6360889.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25744..26187
                     /gene="yjdN"
                     /locus_tag="KKO15_002103"
     CDS             25744..26187
                     /gene="yjdN"
                     /locus_tag="KKO15_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBD6360890.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26320..27108
                     /gene="phnC"
                     /locus_tag="KKO15_002104"
     CDS             26320..27108
                     /gene="phnC"
                     /locus_tag="KKO15_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBD6360891.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27133..28149
                     /gene="phnD"
                     /locus_tag="KKO15_002105"
     CDS             27133..28149
                     /gene="phnD"
                     /locus_tag="KKO15_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6360892.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28255..>28403
                     /locus_tag="KKO15_002106"
     CDS             28255..>28403
                     /locus_tag="KKO15_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBD6360893.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7294 a         7423 c         6937 g         6749 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggttgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggctggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagataaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 cgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgcgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgtttttta tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa
    25021 atatcatgtg aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata
    25081 gaggcaaaaa gattattttc tttttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa
    25141 aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca
    25201 atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac
    25261 cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc
    25321 cggaatgtgc ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg
    25381 ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc
    25441 tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc
    25501 tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga
    25561 aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc
    25621 gcgccgcatc cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca
    25681 gcctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    25741 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    25801 tatcaacgta cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa
    25861 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    25921 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca
    25981 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    26041 aaacgctggt ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    26101 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    26161 gtcgtcaaac aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat
    26221 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    26281 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    26341 gagaagctcg ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt
    26401 catcacagtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    26461 cgtcatttaa gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg
    26521 ggccgcactg tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac
    26581 accggctaca tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    26641 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    26701 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    26761 cagcgcgttt ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg
    26821 atgcagcagg cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca
    26881 gcgcgcatcg tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc
    26941 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt
    27001 caggggcacg tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat
    27061 ctctaccgca gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat
    27121 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    27181 ctcagcaccc tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    27241 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat
    27301 atggagaaga aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt
    27361 atccagggga tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg
    27421 gaagcggtgg atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg
    27481 ccgggttact ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat
    27541 ctgctggcga agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc
    27601 ttcctcgtcc ccggttacta cgtcttcgct aaaaacaata tctccgccag cgacttcaag
    27661 cgcaccgtca acgccgggca tgaaaccaac gcgctggccg tcgccaacaa gcaagtggat
    27721 gttgccacca acaacaccga aaacctcgac aagctgaaaa cctccgcgcc ggagaagctt
    27781 aaagagctga aggtgatctg gaaatcgccg ctgatcctag gcgatccgat cgtctggcgc
    27841 aagaatctgt ccgaaactac caaagacaag atctacgact tctttatgaa ctacggaaaa
    27901 acgccggaag agaaagcggt gctggaacgc ctgggctggg cgccgttccg cgcttccagc
    27961 gacctgcaac tggtgccgat tcgccagctc gcactgttta aagagataca aagcgtgaaa
    28021 ggcaataaag ggctgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag
    28081 ctggatgacc tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg
    28141 gtgcagtaaa tcgtaggtcg gataagacgc cccggcgtcg catccgacaa tgtgtaggcg
    28201 ttgatgccgg atgcggtgca agcaccttat ccggcctaca gaccggagcc aaacatgcaa
    28261 accatcacca tcgccccacc caagcgtagc tggttctcgc ttctgagctg ggccgttgtt
    28321 ctcgacgtgc tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgattaaa
    28381 gacggcggca acatggcaac ctt
//
LOCUS       DAECOD010000048        28330 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.048, whole genome shotgun sequence.
ACCESSION   DAECOD010000048 DAECOD010000000
VERSION     DAECOD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.048"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KKO15_002107"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KKO15_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KKO15_002108"
     CDS             complement(998..2020)
                     /locus_tag="KKO15_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6360894.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KKO15_002109"
     CDS             complement(2034..3536)
                     /locus_tag="KKO15_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD6360895.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4623)
                     /gene="ytfQ"
                     /locus_tag="KKO15_002110"
                     /pseudo
     CDS             complement(3676..4623)
                     /gene="ytfQ"
                     /locus_tag="KKO15_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4933..5463
                     /gene="ppa"
                     /locus_tag="KKO15_002111"
     CDS             4933..5463
                     /gene="ppa"
                     /locus_tag="KKO15_002111"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBD6360896.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5842..6183)
                     /locus_tag="KKO15_002112"
     CDS             complement(5842..6183)
                     /locus_tag="KKO15_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBD6360897.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6186..9965)
                     /gene="tamB"
                     /locus_tag="KKO15_002113"
     CDS             complement(6186..9965)
                     /gene="tamB"
                     /locus_tag="KKO15_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBD6360898.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9962..11695)
                     /gene="tamA"
                     /locus_tag="KKO15_002114"
     CDS             complement(9962..11695)
                     /gene="tamA"
                     /locus_tag="KKO15_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBD6360899.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIPRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11901..12539
                     /gene="msrA"
                     /locus_tag="KKO15_002115"
     CDS             11901..12539
                     /gene="msrA"
                     /locus_tag="KKO15_002115"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBD6360900.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12862..14205
                     /locus_tag="KKO15_002116"
     CDS             12862..14205
                     /locus_tag="KKO15_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD6360901.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14267..14473)
                     /locus_tag="KKO15_002117"
     CDS             complement(14267..14473)
                     /locus_tag="KKO15_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBD6360902.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14798..15352
                     /locus_tag="KKO15_002118"
     CDS             14798..15352
                     /locus_tag="KKO15_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD6360903.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15415..16353)
                     /locus_tag="KKO15_002119"
     CDS             complement(15415..16353)
                     /locus_tag="KKO15_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6360904.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="KKO15_002120"
     CDS             complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="KKO15_002120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBD6360905.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17495..19438
                     /gene="cpdB"
                     /locus_tag="KKO15_002121"
     CDS             17495..19438
                     /gene="cpdB"
                     /locus_tag="KKO15_002121"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBD6360906.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19564..19944)
                     /locus_tag="KKO15_002122"
     CDS             complement(19564..19944)
                     /locus_tag="KKO15_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD6360907.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20033..20893
                     /gene="qorB"
                     /locus_tag="KKO15_002123"
     CDS             20033..20893
                     /gene="qorB"
                     /locus_tag="KKO15_002123"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD6360908.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKILADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21002..21967
                     /locus_tag="KKO15_002124"
     CDS             21002..21967
                     /locus_tag="KKO15_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD6360909.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22075..22737
                     /gene="ytfE"
                     /locus_tag="KKO15_002125"
     CDS             22075..22737
                     /gene="ytfE"
                     /locus_tag="KKO15_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBD6360910.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22782..24194)
                     /gene="cycA"
                     /locus_tag="KKO15_002126"
                     /gene_synonym="dagA"
     CDS             complement(22782..24194)
                     /gene="cycA"
                     /locus_tag="KKO15_002126"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBD6360911.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24502..25122)
                     /gene="fklB"
                     /locus_tag="KKO15_002127"
     CDS             complement(24502..25122)
                     /gene="fklB"
                     /locus_tag="KKO15_002127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6360912.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25341..25886
                     /locus_tag="KKO15_002128"
     CDS             25341..25886
                     /locus_tag="KKO15_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBD6360913.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25870..26517)
                     /locus_tag="KKO15_002129"
     CDS             complement(25870..26517)
                     /locus_tag="KKO15_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD6360914.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26729..27520
                     /locus_tag="KKO15_002130"
     CDS             26729..27520
                     /locus_tag="KKO15_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBD6360915.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27530..>28330
                     /locus_tag="KKO15_002131"
     CDS             27530..>28330
                     /locus_tag="KKO15_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBD6360916.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         6951 a         7543 c         6986 g         6850 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actacaagta agcgtttcca
     4621 catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc gaaaaaacta
     4681 tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt aaataaaacg
     4741 ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta cggttatgga
     4801 ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag acgaaaacaa
     4861 gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca catattttta
     4921 aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc ggaagacatc
     4981 tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat cgacaaagag
     5041 agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc gtgcaactac
     5101 ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact ggtcccgact
     5161 ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttgccgt tctgaaaatg
     5221 accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa gctgagcaaa
     5281 gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc gcaaatcgct
     5341 cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt tgaaggttgg
     5401 gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc aaagaataaa
     5461 taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg gtttttttat
     5521 gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg caaggcttga
     5581 acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt gctggaagtt
     5641 agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc tgcacatttg
     5701 tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag
     5761 caaaagatgg aaaagttaca aaaaaaatcg cgagatacat cgcataaaac aacgccaccc
     5821 gaaggaggcg tgcttttatt attacttatc cctgtctaac cagtcgccgc tttcaattag
     5881 ctttaatcca tcgacgggtc gttgataaac gtacatccat gcactcccat acggcgtctg
     5941 aatcaactgg cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt cggccagcgt
     6001 ggcgttgtca atacgataaa cttcaccgtg taccgttccg ttccccggaa ctgcgcctgg
     6061 atagtggccc aggctataca actggtagtt atcgatactg aaatcgccca gtaactgggc
     6121 attggtcatc cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga caaatattcg
     6181 cattgctaaa actcgaactg atagagcaaa tccagtgcct ggtctacacc agacacggct
     6241 tccagatata gcttaggcat caggcgataa cgtaacgtga gtgttgctat agagtcaaat
     6301 atacccacgc cgtatttcac ttgcagacct ggcaatacat agccgctgac cactacctgg
     6361 gaggagtcgc ctactcccag ggtgtcgagc gctaaattgc ttacgccaaa cgtctcgccg
     6421 attttaccca caatctggcc actttgcgca acccccagac caatcagcat cgaggtcatt
     6481 gccgcactgt cgctctgatc gctctccagc ccttgtccac gtagcaaata agacaatgca
     6541 gcttgttgcg acatcgccgg gtcagagaag atctccgctt tcggttcgtc cgccagacca
     6601 gtgacgcgaa ctccggcgat tacatcgtct tctgtagcat ccgggttacg aatagcttca
     6661 atattaagat acggttgatc cggcggacca gagaacagta actcaccctt acgcacaatc
     6721 agatcctgac cataggcatg gaagcgccct tcagggatgt tgatctgccc gttcaggccc
     6781 agcccttgtt tgtcctgtac gacgttaaga tcgcccgtca gccgcgcttt caggccaaag
     6841 gcgtcaatgc gcacattgtt gccaacgtgg acaatcaggt tactgttaat cggaatcgac
     6901 gccgttttcg gctcttccgg ttgcaggtta tcgttaagca tcaccacatc gctggagacg
     6961 cctactgcgc tttccggcag atcgtgcacc acgatgcgcg cccacgggac atccacgcga
     7021 ccatcgaggg taaacaggtt tggtgtagcc tcgaatacaa catctggcga tacatccatt
     7081 cgtaccatcg gcggcacggt gatccgcact ttactgcctt tcgccgttac tcgtgcccgc
     7141 cagttttcaa tttggctcca gtcggcatta ccgttcaggt agatttcacc ctgctgggtc
     7201 cgtactgtac cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag ctggctcggc
     7261 tgcatatcaa acggcataaa gttgccgtcg atatccacac ccgtaacctg aagctgacca
     7321 aacaactgcg ggctttgcac atcaccaccc agacgcaagt tggcactcac catccccgct
     7381 gctttttccc cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt acggatattg
     7441 acgttgccac caagattacg gcggccttgc ggatcggtca cctgcacctg tccatcaaac
     7501 tggccgttat tggtcaggcg gatggtccag cccaattcgg cacggttgtt acgcaattcc
     7561 gccgtcagat tcagtgtctg aaacgccacc ggcagcgccg catcgttgac ggtttgcgtt
     7621 acctgcacgt tacgccccga aagggtgata ctgccctgcg gcagcccctc tttcgtggtg
     7681 tcccaggcaa catccgcttt acccgtgaag ataccgctgg cctgagtggt ttctggcata
     7741 aacggtttca gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg cccttcggcc
     7801 cccgcatcga tagtttgcgg cacgcacagt tccgcattcg ggttaagcca gcagtgtggc
     7861 ccgatgctga ttttttgctc cttattgcgg taatccagcg caatatcgcg ggtcagcgac
     7921 cacgggccga ccggcgtctg gaagcgggta ttgctaagag ttcccttcca gcgttcttct
     7981 ttgcgatcaa aacttcctgc cagattaagc tgcccggaga caggctcgcc ctgaatccgc
     8041 aactgtagct cgtgctgctt ttcgctgcct ttggcattca gggtgacgag gttgatattt
     8101 acatccggct gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat ctgatcggtg
     8161 gatttaatgt cgccttccac gcgaacctgc gccacggaaa gttcctgcca gcgcaggccg
     8221 cgcgcggtga tatctgccag tagttgtggc gcttccaccg tgccgcgtac tttcaccagc
     8281 cctttcgctg taccgccaag ccccggcagc gcgttatcca gccccggcgc gttgatggtg
     8341 gcatcaagat tgagatcttt tacccccagc tcgcctttca cttcggcact gtttggcccg
     8401 agttccagat gaagcccagg gatcatccac tgcatataac tgttgccttt cagcgtgccg
     8461 tcaacgttca ctttgttctg tttgacgtta ccggtcagct tcagttctgg cacctccatc
     8521 tgccaggtgc cgccgtacag gctaccgcgg gttttaatca agccattgag tttcgacggc
     8581 cactccggga actctttggc ggtgttaatg ccattaagcg ttagctcacc gcgccaacta
     8641 atggcctgct gccagtcgag caacgccttg agttcagttt tcccttccag cgccgcgacg
     8701 gtgagtttgt cgagattgac ctgctgttca ttacctttgg catcaagggt aatggtggct
     8761 ggcgggatct cctgtccctt cactgccgta cgcatagaga gcgtgtaatc ggtcattttg
     8821 ccggtaagtt tcagtttcag atcatccgcc tgatactgct tctcaccagt gaacggccag
     8881 taaagctgtt tgctgttcac ttccacgttg agtggcaatc cggcttccgc cagtcgcgtc
     8941 tgggcgcgta aatccatatc caccggaccg gaaaggttaa cgcctatctc cagctgttcg
     9001 cgcagcgcgc cgcccacttt cagcttcacc ttttcacctt tcaacggctc cacgttcagt
     9061 gtgctgttga gggtgatatc caccggccag ttgtctgaca gctgcgccgt gccgctggcg
     9121 ttgacgatcc cctggttgga atcgatatcc agggcgtcca gtttagtatt gccgtcaatg
     9181 ctgctcactt tcagcagcat ggtgcgcacg gtgatgtccg tgtcgcccgt cacgcgcagc
     9241 tgctcgccct taaactcttc aatgttcagg ttaagcggca aatgcacgtc ggtcatttcc
     9301 ggcaataccg ggcgagaaaa gagatctttc agcgtttcgc cgagcggctt ttcctccggc
     9361 tgcggatttt caattttcgg ttcgaccact tcttcctgcg ccacttccac cactttcggc
     9421 agagcaatca gcaggccttt cagcgacgtc ggtttcaggg tcagggtttt ctcctgccag
     9481 ttcaggccgg aggtgaagtc catcaccgac accgtggtgt catcaatctt gatgttgacg
     9541 ttgtccagcg ccacccgtgt cagggtgatg ggatacggcg tggagagatc cagcggaccg
     9601 ctatcttcct cttcttcaac ctgttcagaa ggaggcattt ttttactgtc gatgttgacc
     9661 tgaatgtctt tcagcgccaa gtcattaata caaacgctac tgttccacag gcactcaaga
     9721 ccgaccccca gatgcagatt acccgctttt accgccacgc ctagctgctc ataacgaacg
     9781 tcagacaagg tgagatcgcg ccagccgccg gtgaccttgc caatatccag tcctggcacc
     9841 cagcgatccg ccgctttaaa tacaagatgc aggccgctgg tggtgcccac cagaaacgcc
     9901 accgatccca gcaacagtaa gataacgata accacgccga ggctgatttt tttccataaa
     9961 ctcataattc tggccccaga ccgatgtaaa actgtaaccc gtgttcgtct ttatccgcga
    10021 ccggtacggc aaaatcgagt ttgattggcc cgaccggcga ttcccagcgt acgccgaccc
    10081 cggtaccggt tttaaagtcg ctgcggggaa tatcgcttac cgcttcgcca ctatcgacaa
    10141 acaccgcgcc ccaccatttt ccggtcacgt tgtactggta ttccagcgat ccggttatca
    10201 acttcgaggc ccctttcagg tcaccgttgg cgtatttcgg ggcgatagat ttgtatttgt
    10261 agccgcgaat actgcgatcg cccccgacga agaaacgcag atccggcggt actttgtcga
    10321 aatcaccggt ttcaatccag cccagcgtgc cgcgtgtcac aaagcgatgg cgatcgtaca
    10381 gtgtgcggat ccagacgtgc tgcgcctgga aaacggagaa atcgacatct gaaccccagg
    10441 ccgtgttgga gtagtcgata gagtagcgtt gcgagtctcc ccaggttggc atcaggccac
    10501 cacgagaacg cgtgcggcta atcatcaccc caggataaaa cagcatcgtg gtgttggtaa
    10561 tttcaccctg ggtaaagtgg tcgaggctcc agcgcaggtt aatggcacgc tgccagccgc
    10621 tggagagatc ccagtagcga gaagccacca gcgtagtgga gtcagattcg gtatcgttca
    10681 ggtcagtgcg cttaaaaccg ccctgcacca aataatattg ttccagtgga ttcttcagca
    10741 gcggcatttt atagctgaag tcgagggtct gttccggcgc ggaaatactg gtactggtgg
    10801 tcaaactgtg accgtatgag ttcatccacg gctttttcca cgtcgctttc acgcgcggtc
    10861 ccacgtccgt agagtaaccg accccggttt cgatggtgtt ttctgttcgc ggcgaaacca
    10921 cgcccgtcaa tggtaatact ttcgtttcgc gcgctttatc aaattgtgga gccaccacca
    10981 ccgagttaaa ccagccggta gcagaaagtc ggcggttcag ttccgccaga tctttcgatt
    11041 catactcatc gccctcttta aacggcacca gattttgcag gtattcatcg cggatttgtg
    11101 atccttcaaa ggtcacatgc ccaaagcggt aacgttcgcc actgttataa tcaatatccc
    11161 agaaggcttt atgcaggccg agcgcaatgc ccagctgcgc tttggtaaat tcgctatcga
    11221 aataaccttt acgcaacgca atactggtta aggacttttt gaaattttca taatcgccct
    11281 ggttcagtac cgtaccaata gccgggcgag tatcgagcaa tttcaaatag tctttatcgg
    11341 tccgcgcgcc gccgcgcaat accacatcgg tgccgccaat taacaccggc acacctggcg
    11401 tgactttggc gatcaatacc tgccgccctt tctttggcgg tggacggaga tcaaattcaa
    11461 tggtcggctg gtaataaccc agcgctttca gaccttcacg gatggcatca tcgacgcgtg
    11521 cgcgaaagcg acggtctggc gtcacttcat cactttcaat cgtagaaagc tgcgcacgaa
    11581 cgttcttttc cagctgtccc gataacccct cgacctgtag acggacgttc gcggcgacgg
    11641 cagatccgct taagcagagt aagcttacac agcataactg tcggatatag cgcacatttt
    11701 ctcctgaata tccttttttc ctgcccctgg aacgccgtta aacggcttaa caaaaatcca
    11761 gtaatatgga ttaaaaagca gactaaaccc caaatatttc ttatgtttta ctttagacct
    11821 attcacggtg gttattgtgt gcaaatacgc ctcttgttac aaccttaacc ccaatgaccg
    11881 attttcggga gagcgacacc atgagtttat ttgataaaaa gcatctggtt tcccccgccg
    11941 atgccctgcc tggacgtaac accccgatgc ccgtagccac gctgcatgcg gtcaacggtc
    12001 actcaatgac caatgtacct gacggaatgg agattgccat ttttgcgatg ggttgtttct
    12061 ggggtgtgga gcgtctgttc tggcagttac ccggcgttta cagcaccgcc gcaggctata
    12121 ccggcggcta tacgccaaat ccgacttatc gggaagtgtg ctccggtgat acgggtcatg
    12181 ccgaagcggt acgcattgtt tacgatcctt ccgtcatcag ctatgagcag ttgctacagg
    12241 tattttggga gaatcacgat cccgcccagg gaatgcgtca gggcaatgac cacggcacgc
    12301 agtatcgttc agcgatttat ccgctgaccc cagaacagga tgccgcagct cgcgccagtc
    12361 tggaacgttt tcaggcggcg atgcttgccg ccgatgacga tcgtcacatc accacggaaa
    12421 tcgctaacgc cacaccgttt tattatgccg aagatgacca ccagcaatat ctgcataaaa
    12481 acccatatgg ttactgtgga attggcggaa ttggcgtctg tctgccgccg gaagcatagc
    12541 gttacgggta caaatgtagt ttgttgataa agtgctcttt gtctatgcct gatgcggctt
    12601 aaacgactta ccaggcctac aaaatcgccc aaattcaata tattgcaagg actgcgtagg
    12661 cctgatcggc atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct
    12721 ctggcgactt tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt
    12781 cgaatcggct ttcaatgtgt atttcacaca aattaatcaa ctttcccttc cgagggtctg
    12841 gcctgaaagg tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct
    12901 gtaagtgcgt tcttctcgat gtccgagatc tcgcttgccg cctcacgcaa aatcaaactt
    12961 aaactgctgg ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat
    13021 cccggcatgt tctttaccgt ggtccaaatc ggtctgaacg cagtggcgat tctcggcggt
    13081 atcgtcggtg atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg
    13141 gcagagctct ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt
    13201 atcctgtttg cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct
    13261 ttgcgtatca tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc
    13321 ttcaacggcc tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat
    13381 gacatcactt ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta
    13441 cgtaaacagg aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg
    13501 tcttcaatga caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc
    13561 ctgaagaata aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt
    13621 gaccacatca tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa
    13681 agcctggcac tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta
    13741 accctttcag aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg
    13801 aacgagtacg cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg
    13861 ggcgatctgg tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg
    13921 ctgattgacg gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc
    13981 ccgcagtcgg gcaactacga aaccatcggc ggctttatga tgtttatgct gcgtaagatc
    14041 ccgaaacgca ccgattcggt gaaattcgcc ggctacaaat ttgaagtggt ggatatcgat
    14101 aactaccgca tcgaccagct gctggtgacc cggatcgaca gcaaggccac cgccctttcg
    14161 ccaaaactgc ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca
    14221 acggctcctg aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg
    14281 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    14341 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaatg tccttgatgt
    14401 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    14461 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    14521 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    14581 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    14641 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    14701 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    14761 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    14821 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa tcggtcagcg
    14881 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    14941 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    15001 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    15061 gttaccgcac gatcgttacc agaccaccac cattgttaac accgacgacg cgattccggg
    15121 ttccggcatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    15181 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    15241 cgctgtagtg gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgctcac
    15301 accggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaata
    15361 acataaattc tggaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    15421 ctctgagttc ttacaccgaa ctaatactgg taacgctagt tgctcttttt taaacgcctc
    15481 aatggtcgat ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    15541 attcgcactt actatcgcat ccacttgttc ggctaattcg acttgttggc aacctgcatt
    15601 aaccagaaaa ttataaataa gagagtaatc ttcatcctcc aaaacaacag aggaaccatt
    15661 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    15721 ggcaaaatta tcatcaatta ttttttcgta aatataccat ccatcgcatt ctttaattcc
    15781 accgcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta catcctccaa
    15841 taaggcataa ccgaaagata cgtcaccaaa cgcctggcga atataacctt taatcctttt
    15901 tggtatgtca gactgttttt tctgcaaaat atcacaggca tacaatcttc tgttttgttc
    15961 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    16021 aatttcttcg tattcaaacc cactgcctcg caatgttatt gtatattcag caccgttatc
    16081 tttaaggctg aaactgataa gaaaattatc catggcatta acagtttcat tttttgatga
    16141 aagcacttga taactatttt tatcaatctg tgttgcgcaa cgaaaggact cccgcaaagt
    16201 taacagacta tcataattcg aaacccaatc ttttttctta aatgcatgct gaatattatt
    16261 aaaaaaatcg tttgaatgtg tttttgtatg cgatgacttt acaaataact ccaccgcacc
    16321 aataaaatta ttgtgtctga tcctgggtaa catatggcta ccttatttta tttggttatt
    16381 ttatgtcacc attaaatatc tcattataga agtaaactat caaaaaacgc tcattattaa
    16441 aatgtaactt actagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    16501 ctttttaggt tgggataaat caggatggca gaatcaggaa atacactgtt tctgccatct
    16561 gaatttagta aatggacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    16621 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    16681 cggcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    16741 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    16801 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    16861 ggcgcgcatc gcggacctga atcagcttgc gcacaccgca ctcttctttc caggccttgc
    16921 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    16981 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    17041 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    17101 cgggaggatc ttcttcagaa aggaccggaa tatccggtgc cagcgtacgt aaaccgtcca
    17161 tgataacggt gtgagcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    17221 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    17281 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    17341 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    17401 aaagcgtaaa attgtggcat tcttcactgt tctataagta aggcgtttat tctccctttt
    17461 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    17521 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    17581 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    17641 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    17701 cgtactggtc gataacggcg atttgattca ggggagtccg ctggccgatt acatgtcggc
    17761 gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    17821 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    17881 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    17941 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    18001 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    18061 agctaattta tccggaaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    18121 gcctgaaatg cgcgagaaag gtgccgatgt cattgtcgtt ctggcgcatt ccgggctgtc
    18181 tgccgatccg tataaagtaa tggcggaaaa ctcagtttat tacctcagtg aaattccggg
    18241 cgttaacgcc attatgtttg gccatgctca cgccgttttc ccgggtaaag attttgctga
    18301 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
    18361 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
    18421 gcaggtgacg caggcgaaag cggaagcacg gccgatttac gacatcgcca ataaaaaatc
    18481 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
    18541 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
    18601 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
    18661 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
    18721 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
    18781 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
    18841 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattgc
    18901 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
    18961 tgatgttatt gatggtgtga attatcagat tgatgttacc caacccgccc gctatgacgg
    19021 cgagtgccag atgattaatg ccaatgcaga gcggattaag aacctgacct ttaatggcaa
    19081 gccgattgat ccgaacgcta tgttcctggt tgccaccaat aactatcgcg cttacggcgg
    19141 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
    19201 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc atccggcggc
    19261 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
    19321 aacttccccg tcagataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
    19381 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
    19441 cacttctttt tcggcctata aatcaaccgc atccggcatt tattggcgga tgcgatgctg
    19501 ccgtatctta tccaccctac aagccatgaa ccgtagaccg gataagcgca gcgcatccgg
    19561 cagttatgcc gcacgttcat cccgcaccgc caacacctcg ggcaaattca actcaatcca
    19621 gtccgccagt gcagcaacct tttcgctcac ctgctcgccc agcggcgtga ggctatattc
    19681 cacatgcggc ggcaccaccg gatacgcgat acggttaaga aaaccatcct gttctaacgc
    19741 ctgtaacgac tgcgcaagca tcttttcact caccccaccg attttgcgcc gcaggtcgct
    19801 aaagcgatga gtaccttcgc gtagcgccac cagaatcaac accccccaac ggctggtgac
    19861 gtgtttcaac acctcgcgcg acgggcactg ttccgcaaag agattaccct ctttcagttg
    19921 ttgtgacagg ctaacctgac tcatttcata cttacctttt tgcacgtact tactaaaagt
    19981 aagtttaggt gttagcgtat ttaaacacaa gacaaaacga tggagacttc ccatgatcgc
    20041 tattactggt gccactggcc aacttggtca ctatattatt gaatccttga tgaaaacggt
    20101 tcctgccagc caaatagtgg ctatcgttcg taatccggca aaagcccagg ccctggcagc
    20161 acaaggcatt accgtgcgtc aggctgacta cagcgatgaa gccgcactga catctgcact
    20221 tcagggagtg gaaaaactac tgctgatctc ttccagcgaa gtgggtcaac gtgccccgca
    20281 gcatcgtaat gttattaatg ccgcaaaggc ggctggcgtg aaatttatcg cttataccag
    20341 cctgctacat gcagatacct ccccgctcgg cctcgccgat gagcacatcg agacggagaa
    20401 aatactggct gattctggca tcgtttacac cctgctgcgc aacggctggt acagcgaaaa
    20461 ctacctcgcc agcgccccgg cagcactgga acacggcgta tttatcggtg cggcgggcga
    20521 tggcaaaatc gcctcagcaa cgcgggcaga ttatgcgaca gctgcggcac gcgtgattag
    20581 cgaagccggt cacgaaggca aggtttacga actggcgggc gatagtgcct ggacgttgac
    20641 acagttagcg gcagagctga ccaaacagag cggcaaacag gttacctatc aaaatctgag
    20701 cgaagccgat ttcgccgcgg cactgaaaag cgtcggactg cccgacggac tggcggatat
    20761 gctggcggat tctgacgttg gcgcatcgaa aggtggtctg tttgataaca gcaaaacgct
    20821 tagcaaattg attggctgcc caacgacaac gttagccgaa agcgtaagcc atctttttaa
    20881 tgttaataac tagttaatta aagtggcatc ctcccgcatc ctctctgata atgacgggat
    20941 gccgggagca atcatgtctg cttcctgaac gtttcttctg acagaccaat ggatgccagt
    21001 aatgattagc ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt
    21061 cgggccgttg atcgtgccgg aatacccggc gatgttgcag tcgatggggc gttatctggc
    21121 gttagggtta attgcgctac ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc
    21181 gcgtcgggac tggcttaccg ccttgatgct cactatgatg ggcaacctca tttattactt
    21241 ctgccttgcc agtgccattc aacgtactgg cgcgcctgtt tccacgatga ttatcggcac
    21301 cctgccggtg gtgattcctg tctttgccaa tctgctttat agccagcgcg acggcaaact
    21361 cgcgtgggga aaactcgccc cggcactgat ttgtattggc atcggcctgg cgagtgtgaa
    21421 tattgctgag ttaaaccacg gactccccga ttttgactgg gcacgttata cctctggcat
    21481 cgtgctagcg ttagtttccg tggtctgctg ggcatggtat gccctgcgca acgcccgctg
    21541 gctgcgggaa aatcccgaca aacatccgat gatgtgggcg acggcgcagg cgctggtcac
    21601 gctgccggtt tctctcatcg gctatctcgt cgcctgttac tggctgaata tacaaacgcc
    21661 ggacttctcc ttaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat
    21721 agccgtgctt tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc
    21781 gacagtgatt ctcgggccgc tgattgtttt cgaaacgctg gcaggtttgc tgtacacctt
    21841 tttactccgc cagcaaatgc cgccgctaat gacgctgagc ggtatcgcgc tgttagtgat
    21901 tggcgtggtc atcgcggtca gagcaaaacc agaaaaacct ttaactgaat ctgtctcaga
    21961 aagttgacat gctggcagtg agttaaataa gcctctgcta cgtaagggtt atagcttttg
    22021 ccttaaaggt gtatttaaaa tacatcttat cttattaaga atgaggtatc agctatggct
    22081 tatcgcgacc aacctttagg tgaactggcg ctctctatcc ctcgcgcttc ggctctgttt
    22141 cgtaaatatg atatggatta ctgctgtggc ggtaagcaga cgctggcgcg cgcggcggca
    22201 cgtaaagaac tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg
    22261 attgagaaag actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc
    22321 taccacgatc gtcaccgcga gcagctgccg gagctgatcc tgcaagcgac taaagtcgag
    22381 cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga caaaatacct gaccatgctg
    22441 catgaagagc tttccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa
    22501 caaggcatgg gcagccaggc aatggggcca atcagcgtaa tggaaagcga gcacgatgaa
    22561 gcgggcgaac tgctggaagt gatcaaacac actaccaata acgtcacacc gccgccagaa
    22621 gcctgcacca cctggaaagc gatgtataac ggcattaatg aactgattga tgacctgatg
    22681 gatcacatca gtctggaaaa caatgtactg ttcccacgcg ccctagcggg tgagtgatct
    22741 aaaagctgga tggcattgcg ccatccagca tgataatgcg gttatttccg cagttcagca
    22801 gcccgcttct taccaataaa cagccagccc aaccccagcg cgataaacca cagcggggta
    22861 accagcagcg cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag
    22921 aacgccatac atacccagca catcagcttg ccgagcggca tcttgtagat cgacttttca
    22981 tgcagatgag gacgctgttt gcggtacacc aggtacgagc aaaggataat cgtccagacg
    23041 aacataaaca gaatcgcgga aacggttgta atcatcgtga acgcgccaat cacgctagga
    23101 ttcacataca acatcaccac gccgccgagc agacagatac acgagaaggt caaccctttc
    23161 gcgggtactg cgcgcttaga aagtttagcg aacgctttcg gcgcaacacc ttcctgcgcc
    23221 agaccaaaca gcatacggct ggtagagaag acaccgctgt tagcggaaga cgccgcagag
    23281 gtcagcacca caaagttgat cacgctggca gccgcaggca aacctaccaa cacaaacagc
    23341 tcaacgaacg ggcttttctc cgggactacc gaactccacg gtgtcacgga cataatcaca
    23401 atcagcgcga agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc
    23461 agtgatttct ccggatcttt ggtttccgca gctgttgtac ctaccagctc aatccccacg
    23521 aaagcgaaaa ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag
    23581 ccgccgtcat tccacaaatg cgcgaatgac gcttccacac cggtcggtga ctgaaagtgc
    23641 atcgccacca tgaccaggcc gacgacaatc agcgacacga tggcgacgat tttgatcatc
    23701 gcaaaccaga actccatctc accgaacatt ttcacggtgg caaggttgag cgtcagcagc
    23761 agcactatca ccgccagcga ggcgacccag tcggagagat cggggaacca gaactgagca
    23821 taagccgtga tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc
    23881 cagccggtga aatatcccgc ccacggcccc agtaaatcgg aagcgaagtc actaaaagat
    23941 ttgtattcca gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata
    24001 aaaccaatga tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgccg
    24061 gaccccataa acaacccagt accaatggca cctccaatgg caataagctg aacatgtcgg
    24121 tttgtgagat tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt
    24181 acctgatcta ccatgttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct
    24241 ttagtcagtc tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac
    24301 gcgggatgaa tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata
    24361 tcacggtgat aacgcgaaac agctcacaaa aatacacgct tacggggtct ttgcaacaca
    24421 aaatagcgcc gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc
    24481 gaggggaaca gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt
    24541 gctgaacgga gggatggatg cacctgcgcc gcgctcgcca tatgccagtt cctgcgggat
    24601 agtcagttcc catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat
    24661 cacgccatta accgggaatt cagcgggttc accacgagca acggagctgt caaacacggt
    24721 gccgtcgatc agtttaccgg tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc
    24781 gccttcaccc tggttgatca cgcggaattg caggccagat tcggtgctat tcacaccttc
    24841 ttttttggcg ttttcttcca ggtatttcac gccttcagca gccatcgcct ggaaacgctg
    24901 acgacgaacg gcatcggcgc gttcgtggat ttcacgcagc gcgcgatgca ccacatcaac
    24961 cggcacagcc ggatgtttgc cttccagcgc atcggcaata cctgcaacca gtgcttctgg
    25021 cagcagccct tccaggccag attcactcag ttgttgccct acctgcaaac caatgccgta
    25081 gcttgcttgc gcttcgatgg tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg
    25141 gtataaaagt agcgggcagc atatcagcca cacttctttg ggtaaaactt tgtcgcagga
    25201 atgatgacaa atcccgggga aaaagaaaca atagcaatat cccgtatgaa tataataaag
    25261 gcgggtttac cacggacatc atagcgttag cttattcata ggctatgatt gaggaacaag
    25321 acgtggagca ggaggaaacc atgcccgggc gctttgaact aaaaccaacc ctggagaaag
    25381 tctggcacgc gccggacaat tttcgcttca tggacccgct gccgccgatg catcgtcggg
    25441 ggatcattat tgccgccatt gtattggtgg tcggatttct gcttccatct gatgatacgc
    25501 ccaacgcgtc ggtggtgacg cgtgaagcgc agttagatat tcagtcgcaa tcacaaccgc
    25561 cgacagaaga acagccgcaa actacccaaa cgcaaccatt ccagccagat agcggaattg
    25621 ataatcaatg gcgctcttac cgtgtcgaac ccggtaaaac gatggcgcag ctgttccgcg
    25681 accacggact gcccgctacc gatgtctatg caatggcgca ggtagaaggc gcgggtaagc
    25741 cgctgagtaa tttgcaaaat gggcagatgg tgaaaatccg ccagaacgcc agcggcgtgg
    25801 tgacgggctt aaccattgat acggggaata atcaacaggt cctgtttacc cgccagccgg
    25861 atggcagttt tattcgtgcg cggtagatga agggtcaacc aaaagccagc taaggattgt
    25921 ctctttgatg accttacgtc tggcttccag tgcctggggg gaaaccatac tgatatgata
    25981 aactaggcca agcgtatgct ggttgatcaa ataatatccc ccaagcgcag caatattaat
    26041 attcacctgc aatgggtcga tacctggctt aaaaatattg tatttcttcc cttcatccaa
    26101 taaggattcc attaactgca agtgagcatg gttaatttct aagagacgct gtgattttgc
    26161 ataatgcaca cctttacttt gattttcact atgcacaatt tttaaaaacc aaggattagc
    26221 aatataataa tcccatgtaa aatcaataat tttctcaatg gctttttgtg gcggaagatc
    26281 tgagaaatca agggcctttt ctttttgtcg aatatcgttc caggtatatt ccagcacttc
    26341 gataaataaa ttttctttat taccaaagtg gtgataaacc gtctgtttac tacaccctgc
    26401 tgcctttaca atattatcta ctctggcccc ttcataacca tattcggcaa attcattaac
    26461 cgcacatagc agcaattttt ccttcaggct ctgtacgttg ctggtatcaa tatacataac
    26521 gcttccccac gacctcttcg tggtgactat cataacctga tttgatttca gcttcagaaa
    26581 aaagggcaaa aacttgtact ttcatcaggt tatcaaatgt cctgtgatcg ccatcgcata
    26641 tcaaccaact agatggttga tctttttatc ctccagtcgc aagctaatca tcaccaagga
    26701 aacaaaattc aaaaaagaga gctattgtat gaaatccact cgtcctgttg ccgtgattac
    26761 cggagccact cgcggtattg gaaagggctg tgcgcaggag cttgctcgtg gcgggtttaa
    26821 cctgctgatt aacgatcgtc ccgatgccga cagcgtagaa aaactgcata tcacacaaca
    26881 agaatgtttg gcggaaggcg tggaagtgat ttgcttccct gctgatgtcg gtgatctatc
    26941 tctgcatgag gagatgctcg acgcggcgca aaaccagtgg gggcggctgg actgtctgct
    27001 caataacgcg gggatttcag tgaaaaaacg cggtgacctc ctcgacctcg aaccagacag
    27061 cttcgatcaa aacattgcca ttaacacccg cgcgccgttc ttccttgctc aggcattcag
    27121 taagcgctta ctggcacagc cgaaaccgga agcggagctg ccgcaccgct cgattatttt
    27181 cgtcagttcc atcaacgcca tcatgctggc gatgaatcgc ggcgaataca ccattgccaa
    27241 aaccgccgtt tctgccgctg ctcgtctgtt tgctgctcgc ctttgtaatg aacaaattgg
    27301 cgtttatgaa gtgcgccctg gtctcatcaa aactgatatg actattccgg caacggctta
    27361 ttacgacgaa ctgattgcca aaggtctggt gccgtgggga cgttggggtt atccggctga
    27421 tattgcttcc accgtccgcg cgatggcgga aggtaaactg atttacacct gtggtcaggc
    27481 ggtcgcaatc gacggcggct taagtatgcc gcgcttctga ggctgaatca tgagagatct
    27541 gttacaacgc ccggatttat tcagtatcaa taccgcgaca ctgggctata aaacgccgct
    27601 acccgccatt attgatgcct gcgccgcacg cggtattggc gcaattgccc cctggcgcag
    27661 ggagttgcag ggtgaagatc tccagcaaat cacccgccag cttgccgcca gtaacatgag
    27721 cgtttccggt ttgtgccgta gcacttacta caccgcgccg acactggcgg aacgcaaact
    27781 ggcgattgat gataaccgcc gggcgctgga cgacgcagcg gtgcttaacg ccgcctgtta
    27841 tatgcaggtg gttggcggtc tgccaacggg cactaaagat ctatatgaag cgcgagaaca
    27901 agtgaaacaa gggattcgcc agttgctgcc acattcgaaa gatgtcggcg tgccaattgc
    27961 gctggagcca ctgcacccga tgacagctgc cgatcgctct tgcctgtgca cgttacgtca
    28021 ggcgctggac tggtgtgatg aactcgatcc cgatggtgag tttggcctcg gcgtggcggt
    28081 ggatgtttat cacgtctggt gggatccgga tctcgccagt cagatcctgc gtgccggaaa
    28141 acgcatcctc gcgtttcatg tttccgactg gttaatgccg accactgatc tggtcaatga
    28201 ccgtggtatg ccgggagatg gcgtcatcaa tattccgtca attcgtcggc tggttgaaaa
    28261 cgccggattt aatggtgcta ttgaactgga aatattctcg ccttactggt ggcagaaaga
    28321 tattaacagc
//
LOCUS       DAECOD010000049        28139 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.049, whole genome shotgun sequence.
ACCESSION   DAECOD010000049 DAECOD010000000
VERSION     DAECOD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.049"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            564..1334
                     /locus_tag="KKO15_002132"
     CDS             564..1334
                     /locus_tag="KKO15_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBD6360917.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KKO15_002133"
     CDS             1325..1798
                     /locus_tag="KKO15_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBD6360918.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KKO15_002134"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KKO15_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBD6360919.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KKO15_002135"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KKO15_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD6360920.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KKO15_002136"
     CDS             3233..3727
                     /locus_tag="KKO15_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBD6360921.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KKO15_002137"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KKO15_002137"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBD6360922.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFNGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KKO15_002138"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KKO15_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="KKO15_002139"
     CDS             7571..8485
                     /locus_tag="KKO15_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6360923.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..9766
                     /locus_tag="KKO15_002140"
                     /pseudo
     CDS             8700..9766
                     /locus_tag="KKO15_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="KKO15_002141"
     CDS             complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="KKO15_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBD6360924.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="KKO15_002142"
     CDS             complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="KKO15_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBD6360925.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12316..13605
                     /locus_tag="KKO15_002143"
     CDS             12316..13605
                     /locus_tag="KKO15_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBD6360926.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAIWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13602..15068
                     /gene="hpaE"
                     /locus_tag="KKO15_002144"
     CDS             13602..15068
                     /gene="hpaE"
                     /locus_tag="KKO15_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBD6360927.1"
                     /translation="MKKVNHWIKGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQDYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15070..15921
                     /gene="hpaD"
                     /locus_tag="KKO15_002145"
     CDS             15070..15921
                     /gene="hpaD"
                     /locus_tag="KKO15_002145"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBD6360928.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15931..16311
                     /locus_tag="KKO15_002146"
     CDS             15931..16311
                     /locus_tag="KKO15_002146"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBD6360929.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16379..17182
                     /gene="hpaH"
                     /locus_tag="KKO15_002147"
     CDS             16379..17182
                     /gene="hpaH"
                     /locus_tag="KKO15_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBD6360930.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17193..17996
                     /gene="hpaI"
                     /locus_tag="KKO15_002148"
     CDS             17193..17996
                     /gene="hpaI"
                     /locus_tag="KKO15_002148"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBD6360931.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18018..19373
                     /gene="hpaX"
                     /locus_tag="KKO15_002149"
     CDS             18018..19373
                     /gene="hpaX"
                     /locus_tag="KKO15_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBD6360932.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19383..20273
                     /gene="hpaA"
                     /locus_tag="KKO15_002150"
     CDS             19383..20273
                     /gene="hpaA"
                     /locus_tag="KKO15_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBD6360933.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20300..21237
                     /locus_tag="KKO15_002151"
                     /pseudo
     CDS             20300..21237
                     /locus_tag="KKO15_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21464..23022
                     /gene="hpaB"
                     /locus_tag="KKO15_002152"
                     /pseudo
     CDS             21464..23022
                     /gene="hpaB"
                     /locus_tag="KKO15_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23040..23552
                     /locus_tag="KKO15_002153"
     CDS             23040..23552
                     /locus_tag="KKO15_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBD6360934.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23945..26095
                     /gene="btsT"
                     /locus_tag="KKO15_002154"
     CDS             23945..26095
                     /gene="btsT"
                     /locus_tag="KKO15_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBD6360935.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26145..26348
                     /locus_tag="KKO15_002155"
     CDS             26145..26348
                     /locus_tag="KKO15_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD6360936.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26359..27315
                     /gene="yjiA"
                     /locus_tag="KKO15_002156"
     CDS             26359..27315
                     /gene="yjiA"
                     /locus_tag="KKO15_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBD6360937.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27384..>28139)
                     /locus_tag="KKO15_002157"
     CDS             complement(27384..>28139)
                     /locus_tag="KKO15_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBD6360938.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6902 a         7357 c         7039 g         6841 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgatttta
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgagcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc gcgggatgtt
     9001 tattaccatt ggtgtactgg ggatttttct cgccatcggc tggtatatgc tctatcgcaa
     9061 ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa
     9121 tgcccgccga gatccgctca attttgccga atggcgcagc ctgttccgta accgcacaat
     9181 gtggggaatg atgctcggat tcagtggcat caactacact gcgtggctgt atctggcctg
     9241 gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc
     9301 ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttactgactg
     9361 gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta ttaccgggat
     9421 gttctgttct gccgccttta cgctgatagt accacaagcg acaacatcca tgatggcggt
     9481 tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct gctggggctt
     9541 gatccacgtc gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc
     9601 cagcttcatc tgcgcctctt tcgcgccgat cattactggt tttattgttg atacaaccca
     9661 ctcattccgt ctggcactaa tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta
     9721 catcttcctg gttcgccagc cgatcaacga cccgcgtaaa gattaaactc cctatgcccg
     9781 ataacatttt gcttatcggg catttttatg gcgattaaaa cgtttcccag ttctccccgc
     9841 tatctgccac tgccatttta cgcggcgtag ctggcgtcac ggtttttacc acagccgatg
     9901 tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct tcggtcaggc
     9961 gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt
    10021 tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc
    10081 tctgctcatc agaagcagaa gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac
    10141 tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt
    10201 tccccaccga gtcttcaatc aggcttttaa tttcacgaac cgcctgggcg ctacgctggg
    10261 ccagattacg cacttctccc gcaaccaccg caaaaccacg tccttgctca cccgcacgcg
    10321 ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggtaatg ccgtcaatta
    10381 cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca
    10441 cgttatctac cactttgccg ccgcgttgcg ccgtttcaga agcacttaat gccagatggc
    10501 tggcctggcg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg
    10561 ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcattattgc
    10621 cggtagcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga
    10681 cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat
    10741 tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt
    10801 caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag acggcgaaga
    10861 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
    10921 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
    10981 actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac tcgttgattt
    11041 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
    11101 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
    11161 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
    11221 cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga
    11281 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtgggt tgctgctggc
    11341 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aaaaacagac
    11401 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
    11461 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
    11521 gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt
    11581 tatcagatac taaaaagtta ttcattatcg cccgggatgt cttcttgtcg ggaattgccc
    11641 agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc agtaaactga
    11701 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
    11761 ccctctttgg tcagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
    11821 actaaaccgt cgcgttccat ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag
    11881 gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat acgcacgatg
    11941 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
    12001 gcttctcgcg cctggagcaa cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa
    12061 aattcaaaat cattaatata gaaacaatta atcatcacgt taccgggact gagctgcccc
    12121 cgcaattctt cattttatca aaattgatga tattttatta acttactgat atttaatatt
    12181 ttattatctt taaatgtaaa tagtttgtta attagatcac atttatatca cttacttttg
    12241 ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta agatgataga
    12301 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
    12361 cttgatgcct ggcaggaagc gttccagcaa tctccctaca aagccccgcc taaaactgcg
    12421 atctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
    12481 cagggtgaaa aagtactgag cggtgcgact gttgcgctga ttgtggggaa aacggcgacg
    12541 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
    12601 agcctgccgg aagagagctt ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc
    12661 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgactat ctataccgag
    12721 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcacag
    12781 ctgctgagcg ccctgagcga atttgccacg ctgaatccag gcgatgctat tctgctcggc
    12841 acgccacagg cgcgcgtgga aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt
    12901 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
    12961 ccaacgctgc cacacccgca cggtacgctg tttgccctcg gcctgaacta cgccgaccac
    13021 gccagcgaac tggaatttaa gccaccggaa gagccgctgg tgttcctgaa agcgccgaat
    13081 accctcactg gcgacaacca gacctccgtg cgcccgaaca atattgaata catgcactac
    13141 gaagcggagc tggtggtagt tattggcaag caggcgcgta acgtcagcga agccgatgcc
    13201 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa
    13261 aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc gatgctttca
    13321 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
    13381 aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg
    13441 atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
    13501 ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg
    13561 ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt
    13621 ggatcaaggg caaaaatgtt gcaggtaacg actacttcca gactaccaat ccggcaacgg
    13681 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
    13741 cagcgaaaga ggcgttcccg aaatgggctg atctgccgat gaaagagcgt gcgcgcctga
    13801 tgcgccgtct gggcgatctg atcgaccaga acgtgcctga gatcgccgcg atggaaaccg
    13861 cggacacggg cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttctcaca
    13921 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg
    13981 acaagatgct caactacacg ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt
    14041 ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata
    14101 ccgcggtgct gaagatgtcg gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg
    14161 cgctggaagc tggtattccg gcgggcgtgc taaacgtggt acaggactac ggcgcaaccg
    14221 caggcgatgc gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg
    14281 ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg gagctgggcg
    14341 gtaaatcacc ggtgctgatt tttgaagatg ccgatattga gcgtgcgctg gacgccgccc
    14401 tgttcaccat cttctcaatc aacggcgaac gctgcaccgc cggttcgcgc atctttattc
    14461 agcagagcat ctatccagaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg
    14521 tgggcgatcc gaacgatccg aatacccagg ttagggcgct tatcagtcag caacactggg
    14581 aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg
    14641 gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa
    14701 cggtgctggc ggacgtagat aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg
    14761 tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgcctggca aacgacgtgg
    14821 agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc
    14881 gtggcattga agcaggcatg gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc
    14941 catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag
    15001 tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg
    15061 gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat
    15121 ctctctgaac taccaggaaa aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa
    15181 gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgatactcac
    15241 tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc
    15301 agcaacgagc tgccgcattt tattcgcgac atgacctaca actacgaggg caacccggag
    15361 ttggggcagc taattgccga ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat
    15421 attcccagcc tgaaactgga atacggcacg ctggtaccga tgcgctacat gaatgaagac
    15481 aagcacttca aagtggtctc catttcggct ttctgcacgg ttcacgattt tgccgacagc
    15541 cgcaagctgg gcgaagcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc
    15601 cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgtgcgga agaagggatg
    15661 aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc
    15721 gaaggccagt tcaaagagtt ttgcaatatg ctgccggagt acgccgacta ctgctacggc
    15781 gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac
    15841 ggcaaggtgg agtttattac cgagctgttc ccaagctctg gcaccggtca ggttaacgct
    15901 gttttcccgc tgcccgcgta aggacgtttt atgccgcact ttatcgtgga atgtactgaa
    15961 aatattcgtg aagaagcccg cttacctgag ctgtttgcca gtgttaacac tgcactggcg
    16021 gcaacgggga tttttccgct tgggggaata cgtagccggg cgcactggat cgatacctgg
    16081 caaatggctg atggcaagca cgattacgcc tttgtgcata tgacgctaaa aatcggcagc
    16141 ggacgcagtc tggaaagccg tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg
    16201 cacttcgcct cactgatgga gagccgttac ctagcgcttt catttgagat tgcagagctg
    16261 cacccgacgt tgaatttcaa acagaacaac gtacacgcgt tgtttaaata aatttgcacc
    16321 agcttccagg ccggatgata aggcgctgcc gcatccggca atgccacagg atatcgctat
    16381 gttcgataaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg
    16441 cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag acgcctacgc
    16501 cgtgcagcgc gaatgggttc gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa
    16561 aatcggtctg acctcgaaag cgatgcaggc aagctcgcag atcagcgaac cggattacgg
    16621 tgcactgctg gacgacatgt tcttccacga tggcagcgat atcccgaccg atcgctttat
    16681 cgtgccgcgc attgaagtgg agctggcttt tgtgctggca aaaccgctgc gtgggccaaa
    16741 ctgcacgctg ttcgacgttt acaacgccac ggactatgtg atcccggcgc tggagctgat
    16801 cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgcaaag tattcgacac
    16861 catttccgat aacgccgcca acgccggagt gatcctcgga ggtcgtccga tcaaaccaga
    16921 cgaactggat ctgcgttgga tctccgccct gatgtatcgc aatggcgtga ttgaagaaac
    16981 cggcgtcgcc gctggtgtgc tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa
    17041 actcgcaccg tatgacgtac aactggaagc cgggcaaatc attctcggtg gttcgttcac
    17101 ccgcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca acatgggctc
    17161 cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagttttaaa gcggcgctga
    17221 aagcaggccg tccgcagatt ggattatggc tggggctgag cagcagctac agcgcggagt
    17281 tactggccgg accaggattc gactggttat tgatcgacgg tgagcacgcg ccgaataacg
    17341 tgcaaaccgt gctcacccaa ctacaggcga ttgcgcccta ccccagccag ccagtggtac
    17401 gcccatcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtaggc acacaaacct
    17461 tactggtgcc gatggtacaa aacgccgacg aagcccgtaa agcggtacgc gccacccgtt
    17521 atccccccgc cggtattcgc ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc
    17581 gcattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa
    17641 cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt
    17701 ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg cagcacccgg
    17761 aagtacaggc cgccattgag caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg
    17821 ggatcctgat cgccaatgag caactggcaa aacgctatct ggaactgggc gcgctgtttg
    17881 tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc cgaagcgctg gcagcacggt
    17941 ttggtaatag ttcttctatt tcgatggcga agcaaaacaa caactctgtc tactaaacgt
    18001 acgatgtgga gcgcatcatg acgacctcta ccctgcaata taaagataat aaaactgttg
    18061 aaattgaaca tcgtgtcatt aataaattat ttcgtcgcct aatcattttt ctttttattc
    18121 tgtttgtttt ttcgtttctt gatcgcatca atataggatt tgccggactg acaatgggca
    18181 aggatcttgg cctgacgtcc acaatgtttg gcctcgccgc aacactcttt tatgtcacgt
    18241 acgtcatgtg cgggatcccc agtaatatga tgttagcgaa aattggcgct cgtcgatgga
    18301 tcgctgggat catggtggtc tggggaattg cttccacctg cacaatgttt gcaacaagcc
    18361 ccgaaacact ctatctactg cgtatgctgg ttggtattgc tgaggcgggt tttttacctg
    18421 gtattttggt ttatctgacc tggtggttcc cggcatatca tcgggccaga gccaatgcct
    18481 tatttatgat agcgatgcca gtcactatga tgctgggatt gatcctttca ggctacattc
    18541 tggccttgga tggactgtgg aatctaaaag gctggcaatg gctctttttg ctggaaggtc
    18601 tgccatcggt cattcttggt gtcgtcacct ggttttatct caacgacaca ccagaccagg
    18661 cgacctggct tgatgaaaac gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag
    18721 agagtgcatt tgcccatgca tctgttccac gctcaacctt acgtgaagtc ctgaccccag
    18781 ccatcatgct atatacactt gcttattttt gcctgactaa tactctgagt gcgattaata
    18841 tctggacgcc gcaaatccta cagagtttca atactggcag tagcaacatc atgattggtt
    18901 tgctggcggc catcccccaa ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt
    18961 ccgaccgtct taaagaacgc aaaaagcata cgattcttcc ctacctgttt gccgctgccg
    19021 gatggttact cgcttctgcg acagatcaca gcctcattca actgagcagc atcattatgg
    19081 cctcaatggg atcatttacc gcgatgacta tcttttggac aacaccggat caagttataa
    19141 gcttgcaatc gcgtgccgtt gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag
    19201 cagtcagtcc attacttatc ggtattttga aagatgcaac cggcagtttt agctcaggtt
    19261 tgtggtttgt agcaggattg ctggtactgg gcgcgttagt catgacccga atccctatgg
    19321 ggagtcggga acactctctt tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca
    19381 tgatgtccag tgaagctatc tttgaacatg gttatgaaaa tattgatttg gtccgggaat
    19441 atgatgcccg ctacgctggt gaagatgtgc attatgaaac atttgcccgg ttagcgaatt
    19501 tctttggacg cgatatgcgc cctcactggc acgatagata ttttcaactc cattatctgg
    19561 tcactggacg cattacttta caactggatg agcacttcta ttctgtgcgc gcaccgcttt
    19621 ttgtactaac accaccatcg gtgccacata ctttctttac tgatcctgat agcgatgggc
    19681 atgttctgac cgtacgtcag gagttaattt ggcccctggt agaaaagctc tggcctggta
    19741 gtggagatgc cgcaatgcta caaggtatct gtctgtcact ggagggaatg gaacatacac
    19801 aggcggcatt aaatcattat tggccgataa tatccctgga gtttcaacaa aataagcaag
    19861 gacgggaaat tctattgcaa tcactggcgc aaagtatttt tacaatccta ctgcgtgaag
    19921 caccaccaaa tgatatttca acatgcagcg tgcgcggcga gatgtgttta ttccagaagt
    19981 ttaatcggct gattgacgaa aattatcgcc aacatttaat ggtccctgaa tatgcaacaa
    20041 tgctcggtat gagtgaatca cggcttaccg agctgtgccg acgttttgcc aatcaatcgc
    20101 cgaagagatt gattttcgaa cgagtgagcc gggaagccag acggcttctg ctctatagcg
    20161 agcaaagcat caaccagata gcccttgatc ttggctacaa agatcccgcc tatttcgccc
    20221 gttttttcaa ccgaatgatg ggttgctcac caacacaatt ccgtggtcgc tgaccattta
    20281 tcaacctgag agactcgaca tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat
    20341 tgcgccagtg gcattcacct acgcctccga accgcaataa atgaaaattg tactacttgg
    20401 aacgaaaggt ggcccttctt tattaaaagc tgaccgttta ccgatggcaa cggcacttat
    20461 tattggcaat aaaacctggt taattgatgc tggttacggg gccagcttac aactggtaaa
    20521 aaataatatt ccactgcgcc agattgataa tattttactc acccatttac acagtgatca
    20581 tattctggac tatccatcag tcttaatgaa tgcatgggct agcggtctgc aggacaaaac
    20641 tattcatgta ttcggtccgc caggcacccg agccatgacc gctgccagtt ggcaggtctt
    20701 cgatcgggac atcacactgc gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt
    20761 tacagctcaa gatgttggtg aaggcgtagt ttataaagat gataccgtga ccgtgactgc
    20821 attaaaggtc cctcattctc ctttccctga cggtgaggct tttgcttatc gcttcgacac
    20881 acaaggcaag cgaattgtct tctcaggaga tacctcgtac tttcccccac tggcaacttt
    20941 ctcagaaggc gctgatatac ttgtgcatga ggccgtacat gagccctccg tcgctaagct
    21001 ggcagacagt attggcaatg gtaaaacact ggcaaaagcc attgcttctc accatacgac
    21061 tattgaggat gtaggcaaaa ttgccaatga agcaaaagtg aaaaaactca tccttagcca
    21121 cctggtacca atcaccgttc cggatgaaac atggaagcaa gaagcacaga aaactattca
    21181 ggccctatcg tcgttggtca tgaaaatatg acgattacca ttccattcgc cccataaaaa
    21241 attccgtccc cgttagccct tgttagcggg gaatattatg atttgcctta ctgtaagttg
    21301 atcacgatcg cagtttacta tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc
    21361 tgaaacgtct cttcccgaca accgcgttca acgcttcaat taaataacaa aaacaacaca
    21421 caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac cagaagattt
    21481 ccgcgccagt acccaacgtc ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg
    21541 tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc accactcatc cggcatttcg
    21601 taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg agatgcagga
    21661 ctctctgtgt tggaacaccg acaccggcag cggcggctat acccataaat tcttccgcgt
    21721 ggcgaaaagt gccgacgacc tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct
    21781 gagctatggc tggatgggcc gtaccccaga ctacaaagcc gctttcggtt gcgcactggg
    21841 cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt atacccgtat
    21901 tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg tcatttgccg
    21961 accgataaag tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc
    22021 gtcagcggtg cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc
    22081 ttcggctcgg cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca
    22141 atggatgccg atggcgtgaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct
    22201 accggctcgc catacgacta cccgctctcc agccgcttcg atgagaacga tgcgattctg
    22261 gtgatggata acgtactgat cccatgggaa aacgtgctga tctaccgcga ttttgatcgc
    22321 tgccgtcgct ggacgatgga aggcggtttc gcccgtatgt atccgctgca agcctgtgtg
    22381 cgcctggcag tgaaactcga cttcattacg gcactgctga aaaaatcact cgaatgtacc
    22441 ggcaccctgg agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac
    22501 accttctggg cattgagtga ctcgatgtgt tcagaagcga cgccgtgggt caacggggct
    22561 tatttaccgg atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg
    22621 aagatcaaaa acattataga acgcaacgtt accagtggcc tgatctacct cccttccagt
    22681 gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg
    22741 aacggtatgg atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc
    22801 agcgaatttg gtggtcgtca cgagctgtat gaaatcaact actccggtag ccaggatgag
    22861 attcgcctgc aatgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg
    22921 atggttgatc gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac
    22981 aacaacgacg atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga
    23041 tgcaattaga tgaacaacgc ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg
    23101 taaatattat caccaccgag ggcgacgccg gacaatgcgg gattacggca acggctgtct
    23161 gctcggtcac ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga
    23221 acccggtttt tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac
    23281 tgatggcgcg ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct
    23341 catgctggca aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg
    23401 aaggagagat ccgcgatgtg caggcgattg gcacacatct ggtgtatctg gtggagatta
    23461 aaaacatcat cctcagtgca gaaggtcacg gacttattta ctttaaacgc cgtttccatc
    23521 cggtgatgct ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa
    23581 ggggcatttt tattgtgatc tcaatcacat atctacccga tgaataattc ttgcccaacc
    23641 gctcaccgcc attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac
    23701 tttacgcgca cgtatctctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa
    23761 acgcaacgca aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg
    23821 gtttgcagga aggcagcaag tgagtgaatc cccgggagct tacaaaagta agtgtcagag
    23881 gtgaacgaac gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt
    23941 tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg
    24001 tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat
    24061 cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc
    24121 ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg
    24181 tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc
    24241 cggtgctggt ccgctggtgg gaccggttct cgccgcgcag atgggctacc tgcctggcac
    24301 gctgtggctg ctggcggggg tagtgctggc cggtgcggta caggacttta tggtgctgtt
    24361 tatctcctct cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatggggcc
    24421 agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt
    24481 tctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct tcaccgtttg
    24541 ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg
    24601 tgtgggtgaa gtctctgtta ttggtatcgt gctgctggtt gcctctatct acttcggtgg
    24661 cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac
    24721 cttcgcgctg attggctatg cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc
    24781 accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg
    24841 tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac
    24901 tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc
    24961 ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc tggctaacga
    25021 aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat
    25081 ggcgctggtt gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga acaccccgcc
    25141 tgctggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat
    25201 catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca gctcctgggg
    25261 cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt
    25321 cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcacg tgttccacaa
    25381 agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct
    25441 gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaagacct
    25501 gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg
    25561 tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct
    25621 gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt
    25681 agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt
    25741 aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact
    25801 gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa
    25861 gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt
    25921 gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat
    25981 cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga
    26041 aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg
    26101 tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag
    26161 gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggtatt ccagactacg
    26221 acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag
    26281 aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct
    26341 gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc
    26401 gcgggcaaaa ctaccctgct gcgccatatt cttaacgaac aacacggcta caagattgcc
    26461 gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactgattgg cgaccgcgcc
    26521 acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggag
    26581 gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg
    26641 gtgattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt tttctcccat
    26701 gagattttat gccagctcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat
    26761 gccgatgagc agatgaacca gttcaccatc gcccagtccc aggtgggcta cgccgaccgt
    26821 attctgctga ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg
    26881 cgcatcaacg cccgcgcacc ggtctacacc gtcacccacg gcgacatcga cctgggcctg
    26941 ctgttcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc
    27001 cactttatcg cggataaaca aaacgatatt tcgtcaattg tggtggaact ggattacccg
    27061 gtagatatca gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa
    27121 ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaatcgcct gctgttccag
    27181 ggcgtccagc gcctctacag cgccgactgg gacaggccgt ggggcgatga aaaaccgcat
    27241 agcacaatgg tgtttatcgg gattcagttg ccggaggaag agattcgggc ggcgtttgcg
    27301 ggattgagga agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt
    27361 acgagtggaa ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt
    27421 ttttcatgtc tcattttcag ttcagccaat ccccattctg aagaattaca aacatctttt
    27481 gttaaataaa aagagctttt actattaagt ttctcataaa tagatttctt tttgataaaa
    27541 tcatagtgtt gtgtttcaga atttttggaa ccacttatga gtgttaaatt acctatttta
    27601 tgcagccagt agacatgctc ttgaggttgg aatctattaa tccagtattc attaactaat
    27661 gcttgaggaa gaatatgctc aattgtgatt ctccccgtat aggttttaat cacgctctca
    27721 tcttgttgct ccatatctaa gcgaagcaaa attgctttta ttaagttcac ttgattcgga
    27781 cgaggctcat ataaatcttc atccagagca gctataaaac ctgaattatc tgcatgttgg
    27841 tttatctgat ttatcacaga atcaaaaggc atatcattat ttattgctac cagagcagaa
    27901 taacaaacca tttctctttg gcttttaatt tgttttttta accacccatg catatatacc
    27961 ttctcaaagg cggtaataaa ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc
    28021 attcgattca tgaatgccat aaccggagga atccactcat ctacccctaa attacttagc
    28081 gatgctattt ttcgtcgtat tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DAECOD010000050        27601 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.050, whole genome shotgun sequence.
ACCESSION   DAECOD010000050 DAECOD010000000
VERSION     DAECOD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.050"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KKO15_002158"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KKO15_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBD6360939.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KKO15_002159"
     CDS             complement(1614..1937)
                     /locus_tag="KKO15_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBD6360940.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KKO15_002160"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KKO15_002160"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBD6360941.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KKO15_002161"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KKO15_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBD6360942.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KKO15_002162"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KKO15_002162"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBD6360943.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KKO15_002163"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KKO15_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBD6360944.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KKO15_002164"
     CDS             7537..8574
                     /locus_tag="KKO15_002164"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD6360945.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KKO15_002165"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KKO15_002165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBD6360946.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KKO15_002166"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KKO15_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBD6360947.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KKO15_002167"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KKO15_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBD6360948.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="KKO15_002168"
     CDS             10745..11626
                     /locus_tag="KKO15_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6360949.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KKO15_002169"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KKO15_002169"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBD6360950.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="KKO15_002170"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="KKO15_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBD6360951.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KKO15_002171"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KKO15_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="KKO15_002172"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="KKO15_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBD6360952.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="KKO15_002173"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="KKO15_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBD6360953.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="KKO15_002174"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="KKO15_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBD6360954.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="KKO15_002175"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="KKO15_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBD6360955.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="KKO15_002176"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="KKO15_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBD6360956.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="KKO15_002177"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="KKO15_002177"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBD6360957.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="KKO15_002178"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="KKO15_002178"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBD6360958.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="KKO15_002179"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="KKO15_002179"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBD6360959.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="KKO15_002180"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="KKO15_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBD6360960.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="KKO15_002181"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="KKO15_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBD6360961.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="KKO15_002182"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="KKO15_002182"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBD6360962.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="KKO15_002183"
     CDS             24893..25273
                     /locus_tag="KKO15_002183"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBD6360963.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="KKO15_002184"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="KKO15_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBD6360964.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KKO15_002185"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KKO15_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBD6360965.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="KKO15_002186"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="KKO15_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6809 a         6868 c         7235 g         6689 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggtgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAECOD010000051        27553 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.051, whole genome shotgun sequence.
ACCESSION   DAECOD010000051 DAECOD010000000
VERSION     DAECOD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.051"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="KKO15_002187"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="KKO15_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBD6360966.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="KKO15_002188"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="KKO15_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBD6360967.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="KKO15_002189"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="KKO15_002189"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBD6360968.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="KKO15_002190"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="KKO15_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBD6360969.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="KKO15_002191"
     CDS             5718..6659
                     /locus_tag="KKO15_002191"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBD6360970.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="KKO15_002192"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="KKO15_002192"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBD6360971.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTIIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="KKO15_002193"
     CDS             7679..8929
                     /locus_tag="KKO15_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD6360972.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="KKO15_002194"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="KKO15_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="KKO15_002195"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="KKO15_002195"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBD6360973.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHSAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="KKO15_002196"
     CDS             10866..12116
                     /locus_tag="KKO15_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD6360974.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTAQVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIARLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="KKO15_002197"
     CDS             complement(12172..13260)
                     /locus_tag="KKO15_002197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD6360975.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="KKO15_002198"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="KKO15_002198"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBD6360976.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="KKO15_002199"
     CDS             complement(14194..15243)
                     /locus_tag="KKO15_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBD6360977.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="KKO15_002200"
     CDS             15342..16223
                     /locus_tag="KKO15_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6360978.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="KKO15_002201"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="KKO15_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBD6360979.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="KKO15_002202"
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="KKO15_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBD6360980.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KYVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="KKO15_002203"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="KKO15_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="KKO15_002204"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="KKO15_002204"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBD6360981.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="KKO15_002205"
     CDS             21991..23067
                     /locus_tag="KKO15_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBD6360982.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="KKO15_002206"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="KKO15_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBD6360983.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="KKO15_002207"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="KKO15_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBD6360984.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="KKO15_002208"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="KKO15_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBD6360985.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..>27553
                     /gene="mglC"
                     /locus_tag="KKO15_002209"
     CDS             27124..>27553
                     /gene="mglC"
                     /locus_tag="KKO15_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="ABC transporter; functions in galactose transport;
                     part of MglA2C2B transporter complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-methylgalactoside transporter permease"
                     /protein_id="HBD6360986.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMI"
BASE COUNT         6718 a         6630 c         7367 g         6838 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctaggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gcggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagatc cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tagctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatctcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgataatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgaccg gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgcaggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 attcggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcacaggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc ggcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcagata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaatacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agactttgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa taaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tca
//
LOCUS       DAECOD010000052        27063 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.052, whole genome shotgun sequence.
ACCESSION   DAECOD010000052 DAECOD010000000
VERSION     DAECOD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.052"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..458
                     /locus_tag="KKO15_002210"
     CDS             <1..458
                     /locus_tag="KKO15_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6360987.1"
                     /translation="GDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN"
     gene            complement(467..607)
                     /locus_tag="KKO15_002211"
     CDS             complement(467..607)
                     /locus_tag="KKO15_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBD6360988.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            849..1142
                     /locus_tag="KKO15_002212"
     CDS             849..1142
                     /locus_tag="KKO15_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBD6360989.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1214..1873
                     /locus_tag="KKO15_002213"
     CDS             1214..1873
                     /locus_tag="KKO15_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBD6360990.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1965..2411
                     /locus_tag="KKO15_002214"
     CDS             1965..2411
                     /locus_tag="KKO15_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBD6360991.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KKO15_002215"
                     /pseudo
     CDS             complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KKO15_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3891..4310
                     /locus_tag="KKO15_002216"
     CDS             3891..4310
                     /locus_tag="KKO15_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD6360992.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4310..5578
                     /gene="umuC"
                     /locus_tag="KKO15_002217"
     CDS             4310..5578
                     /gene="umuC"
                     /locus_tag="KKO15_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD6360993.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KKO15_002218"
     CDS             complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KKO15_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBD6360994.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KKO15_002219"
     CDS             complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KKO15_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBD6360995.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8062..8781
                     /gene="fadR"
                     /locus_tag="KKO15_002220"
     CDS             8062..8781
                     /gene="fadR"
                     /locus_tag="KKO15_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBD6360996.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8833..10365)
                     /locus_tag="KKO15_002221"
     CDS             complement(8833..10365)
                     /locus_tag="KKO15_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBD6360997.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10695..11993
                     /gene="dadA"
                     /locus_tag="KKO15_002222"
     CDS             10695..11993
                     /gene="dadA"
                     /locus_tag="KKO15_002222"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBD6360998.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12003..13073
                     /gene="dadX"
                     /locus_tag="KKO15_002223"
     CDS             12003..13073
                     /gene="dadX"
                     /locus_tag="KKO15_002223"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBD6360999.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(13241..14655)
                     /locus_tag="KKO15_002224"
                     /pseudo
     CDS             complement(13241..14655)
                     /locus_tag="KKO15_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15266..17002)
                     /locus_tag="KKO15_002225"
     CDS             complement(15266..17002)
                     /locus_tag="KKO15_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBD6361000.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KKO15_002226"
     CDS             complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KKO15_002226"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBD6361001.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            18111..18722
                     /gene="emtA"
                     /locus_tag="KKO15_002227"
     CDS             18111..18722
                     /gene="emtA"
                     /locus_tag="KKO15_002227"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBD6361002.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18724..19458)
                     /locus_tag="KKO15_002228"
     CDS             complement(18724..19458)
                     /locus_tag="KKO15_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBD6361003.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKTAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHIIITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19659..19913
                     /locus_tag="KKO15_002229"
     CDS             19659..19913
                     /locus_tag="KKO15_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD6361004.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19963..21423)
                     /locus_tag="KKO15_002230"
     CDS             complement(19963..21423)
                     /locus_tag="KKO15_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD6361005.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21360..21917)
                     /locus_tag="KKO15_002231"
     CDS             complement(21360..21917)
                     /locus_tag="KKO15_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBD6361006.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21943..22797)
                     /locus_tag="KKO15_002232"
     CDS             complement(21943..22797)
                     /locus_tag="KKO15_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBD6361007.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22797..23606)
                     /locus_tag="KKO15_002233"
     CDS             complement(22797..23606)
                     /locus_tag="KKO15_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBD6361008.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23616..24374)
                     /locus_tag="KKO15_002234"
     CDS             complement(23616..24374)
                     /locus_tag="KKO15_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6361009.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24371..25351)
                     /locus_tag="KKO15_002235"
     CDS             complement(24371..25351)
                     /locus_tag="KKO15_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBD6361010.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25351..26373)
                     /locus_tag="KKO15_002236"
     CDS             complement(25351..26373)
                     /locus_tag="KKO15_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBD6361011.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6797 a         6586 c         7002 g         6678 t
ORIGIN      
        1 caggagacat cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg
       61 acctatggtc acgtgccgtt attggctggt caatgtcgcc acgcatgacg gcgcaactgg
      121 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc
      181 acacggaccg tggaggccag tactgttcag cagattatca ggcgcaactg aagcggcata
      241 atctgcgtgg aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct
      301 tctttcattc gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa
      361 tgcgggcaac ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt
      421 ggtgtggcgg cctcagtccg gaacaatttg aaaactagaa cctcgcttag gcctgtgtcc
      481 atattacgtg ggtaggatca ctatggcctt tgctgaactc aacattgaca ttcttacccg
      541 cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga
      601 ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc
      661 cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt
      721 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
      781 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
      841 gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
      901 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
      961 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
     1021 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
     1081 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     1141 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     1201 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     1261 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     1321 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     1381 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     1441 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
     1501 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     1561 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc
     1621 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa
     1681 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     1741 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     1801 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     1861 tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc
     1921 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     1981 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     2041 gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca
     2101 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     2161 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     2221 ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc
     2281 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     2341 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     2401 gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc
     2461 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     2521 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     2581 ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga
     2641 gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg
     2701 ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag
     2761 tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     2821 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag
     2881 attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag
     2941 aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg
     3001 cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat
     3061 tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc
     3121 ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa
     3181 tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc
     3241 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     3301 tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac
     3361 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     3421 tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta
     3481 actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     3541 ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca
     3601 tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac
     3661 aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt
     3721 catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga
     3781 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     3841 caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     3901 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     3961 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt
     4021 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg
     4081 gtggaattag tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg
     4141 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     4201 cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata
     4261 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     4321 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     4381 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     4441 aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg
     4501 ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt
     4561 gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt
     4621 ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg
     4681 cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac
     4741 gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     4801 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt
     4861 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     4921 cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag
     4981 aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa
     5041 gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg
     5101 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     5161 ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta
     5221 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     5281 cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     5341 cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     5401 cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     5461 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     5521 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat
     5581 atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat
     5641 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     5701 cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct
     5761 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     5821 ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg
     5881 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     5941 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     6001 ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     6061 accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc
     6121 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     6181 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     6241 attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     6301 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     6361 tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagagc catccacacc
     6421 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     6481 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
     6541 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     6601 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     6661 atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc
     6721 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     6781 gtgaatggca gagattcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc
     6841 aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
     6901 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     6961 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc
     7021 tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     7081 aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa
     7141 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
     7201 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
     7261 tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct
     7321 tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc
     7381 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa
     7441 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
     7501 agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact
     7561 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
     7621 gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga
     7681 gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt
     7741 aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
     7801 aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta
     7861 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
     7921 gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct
     7981 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     8041 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     8101 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     8161 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     8221 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     8281 ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     8341 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     8401 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     8461 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     8521 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     8581 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     8641 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     8701 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     8761 ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt
     8821 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct
     8881 gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt
     8941 caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt
     9001 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     9061 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     9121 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     9181 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     9241 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     9301 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     9361 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     9421 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     9481 gttaaggatg gtatagtgcc agaaggtcgc acagccttcg ttcatcacct gagtctgttt
     9541 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     9601 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     9661 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     9721 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     9781 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     9841 cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata
     9901 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     9961 gcaattgttt ttaaagaaag agttatgtcc atagcaggcg tgtgccatca ccagcgcctg
    10021 catagtaatg gtgttctctt ccatcaagta agcgatacac gggttagagt taatgacgat
    10081 ttcataggcc agcccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
    10141 accgaatgac cagtgcggat agttaattgg catgccgacg ctggaatagg catccatcat
    10201 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
    10261 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
    10321 gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg cacctcattg
    10381 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
    10441 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
    10501 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
    10561 attgccgcag agtcagggag atgtgagtca gctcaccata aaaaagccgt atgttgaata
    10621 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
    10681 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
    10741 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
    10801 ccctggaaac cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
    10861 cagcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg
    10921 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
    10981 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
    11041 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
    11101 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
    11161 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
    11221 atcccgcgct ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg
    11281 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
    11341 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
    11401 gcgtgaagtt tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
    11461 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
    11521 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
    11581 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
    11641 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
    11701 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
    11761 cgatgacgcc agacggtacg ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga
    11821 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
    11881 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
    11941 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
    12001 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
    12061 ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg
    12121 ggcatggtat tgagcgtatc tggagcgcgc tcggggccac cgatggcttt gcattactta
    12181 acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg
    12241 aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg
    12301 tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata
    12361 tttatcttaa agtgaacagt gggatgaatc ggttgggctt ccagtccgat cgcgtgctta
    12421 ccgtctggca gcagttgcgg gcaatggcga atgttggcga aatgaccctg atgtcgcatt
    12481 ttgctgaagc ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg
    12541 cggaggggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg
    12601 aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc
    12661 agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta
    12721 ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc
    12781 gcgatgaaca gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccatg
    12841 cgcctaccgg tactcctgtt ttagtggacg gcgtgcgcac catgacggtg gggaccgtct
    12901 cgatggatat gctggcggtt gatttaacgc cttgcccgca ggcggggatt ggtacgccgg
    12961 ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg
    13021 gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt tgtgacagtg taacttgttg
    13081 taagccggat cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag
    13141 caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt aaaagaatgg
    13201 acactatttc cccggaacct gaactcgccg cacaggcgtt ctacataaaa cgcttacgct
    13261 tcattgttga ctcggagtcg acttcagata aatcacgctt cacccttgat gcagattcgg
    13321 gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca
    13381 ggcccaactg ttgatccagg agctccaggt cctcctgtac cttcacgcgc tccaggagtt
    13441 ccgggtcatc caggaactcc agatccataa ggtcctccag ctcctcgagt ccttccaggt
    13501 caacctagcc ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc tgttttttca
    13561 ggccctcgac ggctttgagc agcgtcggta aattgtcttt gtcccacggg ttaccattac
    13621 agtcaaacac cttcaggctc tggggctgcg gcacctgtac cgttttaaac ttgttttctc
    13681 cacagtagta aattattagt ttgggcggca gccgtggtga agagatcaaa tcgttactag
    13741 aacagtcaaa tactttcagg cgagtcaagt tctccagcat ctcatcaggt attgagttaa
    13801 tattgttgcc ggcacaggat agtttataca ggtactgggg catcgacggc ggtaaaaatt
    13861 gcaagaagtt ttcagaacag ttaatcactc tcaggttagt cgacagctca ggcagccaga
    13921 taatactgtt ttcatagcac ttcagtacca tcagttttga gggctgcacc ttcggtaaca
    13981 agatcaagac gttatctgta cagtcaagca ccagcagcca ctttggcatc accttcggta
    14041 acgcggtcaa cacgttttta ctgcagtaaa attcaatcag atgtggaggc aaattctcag
    14101 ggagtgaagt caatcccagg ttgctaagat tgagcatact ccgtcttgta atgagacatt
    14161 ccttcattct gcgcaatgct tctgcccggt tttcaatccg gcttgctggc aggttttctt
    14221 ccaggatatt ttccttgatt tcttccagac tttctttcag gtcttcttgg ggaatttctg
    14281 actgtttgtt ttgctggttt tcttccagga tttcgtacag gtttttttgc aggttttctt
    14341 gcaaaccatc ctgctgtata gcccattcgc accatttggc gaccatcatg aactcgttat
    14401 gttgctgatt cagacgagtc cccaacgtga tgatggtatt gttgtccacc tgtatgttca
    14461 ctttgcccgc gatactgagg tcaatgatct gccagggggc atcaccccag ctgaattttt
    14521 gcgggctgag atacttcatg ttctgcaaaa tttgattctt gagaagagcc gccctggcgg
    14581 gcgtcgtacg cctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc
    14641 tttaaagcta gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag
    14701 acgtgaatgt ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc
    14761 atcgttgact ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg
    14821 gcctaggtag ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt
    14881 tattaagcaa aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga
    14941 aacgcctatt gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg
    15001 atgtcaggca ggggcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt
    15061 tacagtcgga aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc
    15121 agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata
    15181 tcaaaatcag gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca
    15241 tccggcgata gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc
    15301 caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact
    15361 ctacctggtc accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg
    15421 tttgctgttt atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag
    15481 catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg
    15541 gtggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac
    15601 acaatacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac
    15661 gaaacagggc tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta
    15721 cacaccattt atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa
    15781 tatccaggcc aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt
    15841 tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg
    15901 cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac acggctagga
    15961 tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga
    16021 aaccgcggaa agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc
    16081 atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata
    16141 gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca
    16201 ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag
    16261 ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta
    16321 atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta
    16381 aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca
    16441 cgaacatcca actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca
    16501 gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt
    16561 cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga
    16621 tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc
    16681 cggttaaacc agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg
    16741 ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg
    16801 ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat
    16861 cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg
    16921 aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc
    16981 taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa
    17041 ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac
    17101 attttaagaa caggatgacc gctaatcgtc aactgagcgc cttcccgggt gttattcaga
    17161 atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg
    17221 gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa
    17281 cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga
    17341 cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc
    17401 acgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca
    17461 atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc
    17521 gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg
    17581 tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta
    17641 aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt
    17701 ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct
    17761 gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta
    17821 gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc
    17881 tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc
    17941 tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga
    18001 aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag
    18061 gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga
    18121 gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga
    18181 acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc
    18241 agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg
    18301 aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa
    18361 aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga
    18421 ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc
    18481 tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg
    18541 tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg
    18601 cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc
    18661 atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt
    18721 aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat
    18781 ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg
    18841 agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg
    18901 ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat
    18961 accttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga
    19021 caaatcatac aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata
    19081 aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt
    19141 gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc
    19201 aacagtaaac tcgactttcg caccctgagt ttcggcggta atgataatgt gctgtgcctt
    19261 tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt
    19321 tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc
    19381 ggttttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg
    19441 ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg
    19501 tcatagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc
    19561 agccgacaaa cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc
    19621 atttcttgcg accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta
    19681 tttttggcct gatagccggc attatcggca agctaatcat gccggggcgt gatggtggtg
    19741 gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga
    19801 ccatgtttgg cattgacggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg
    19861 tggtgggcgc tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc
    19921 ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac
    19981 acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc
    20041 aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg
    20101 aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga
    20161 ggaacgcgcc tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca
    20221 tgcggtcatt gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc
    20281 agcgtggatc agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat
    20341 accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc
    20401 attgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa
    20461 tccccagtcg cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg
    20521 cttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg
    20581 ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt
    20641 ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata
    20701 atgggtgatg ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata
    20761 gctgattccc gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag
    20821 cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg
    20881 tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc
    20941 cagttgtaat ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt
    21001 tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag
    21061 ccgacttttc agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc
    21121 tttgtcatac tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg
    21181 cgggttatct ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg
    21241 cggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt
    21301 gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt
    21361 tagctggcta ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg
    21421 catatcgtag gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc
    21481 ttcagggtgg tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa
    21541 taccctttgg aaacttcgac tgcccccaga ttgttggtca gaatccgcgc cagatcgagg
    21601 ttgccgtcat aggggacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta
    21661 tcaaagccac gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg
    21721 ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt catggtgggt
    21781 tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg
    21841 ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt gcagagataa
    21901 cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag aggctgtggg
    21961 ttgcagactg actttaatgt cagctggtgc cgcgtaataa ggcgcggagg taataaaaag
    22021 gcgaatgccg cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc cggtcagagc
    22081 cagcgtgcag tgaggagcca gcgacggcgc tatttgcgca atttcagttg cctgttgcgg
    22141 actaaatttg tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt
    22201 cggcgtgtcg gcttcaacaa caattttctt ctcgggtgcg tggcgacgta actgagcgat
    22261 tgcgcctgac cagccctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat
    22321 ggtttccgca catccggcac gatgaattag tcctccggca gccagaattg cctgcgaagc
    22381 cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattgccgt caggataacg
    22441 ttcacgaagc aatgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag
    22501 cacattctgg actgccttcc atccctgatg aagtgctgcg gcattaccct gcgcacggat
    22561 tagccgttga cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc
    22621 cagcgtagtt aacatcttac acgcgacaga aacgccgctg acgcagccgc cctgacggtg
    22681 aaaaaacgct atatagccag gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc
    22741 tccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac
    22801 agtgcctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg
    22861 agggcacgag catcttgttg ttgataccag tgatataacc gctcacgctc atcgtcgtta
    22921 agcgcgccaa gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt
    22981 tggcaatttt gtccacggat aaaatcaacg tgggcataaa ttcccatctg atacaggacg
    23041 ttgagcgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg
    23101 gctggagaga cgaacgaggt gctgaccaga tgagttgtat agacgcgaag ccgtgcctga
    23161 ttattcagtt tgctcattgc atggcgcata tcagccacca gagtggagcg ggaagccacg
    23221 gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg
    23281 acattgtcag ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg
    23341 taatcaacgc cataaaccgt agtgagtttg tcagccagcg ccagcgatac ggtgcctggt
    23401 ccgcagccca tatcgaataa ggtctgagcg ccttgcaggt caattttagc gatcagttgt
    23461 tgcaggtagc tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc
    23521 cagtgatcgg gcgttttttc tgtgcggtga gccagtttga gttgctgcag ataaaggtct
    23581 gcaaaatcaa tatcatcaat gagcatgatt gttccttagc ggctaattgc agagaggtga
    23641 tgatgaattt ggtcggcaga aacccgatac agggccgcca gtttgttcgt tgttaactgt
    23701 tcggtcggta agccttgtgt tactcgtcca tctggctcta cttggataat gctgtcggca
    23761 atggcgttgg catggagcgg atggtgagtc gacattaaca ttgtcatgcc gttatttttg
    23821 agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcgggc
    23881 tcatccagta acaacaagcg cggttgttgg acgagtgcac gggcgattaa agccagctga
    23941 cgttcaccgc cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt
    24001 tccagttgct ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg
    24061 gtgggggcca ggcccatcaa caccatatcc agcacactga aagcaaaaat accgtcgtgc
    24121 gcctgcggca cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag
    24181 aggctatcca gtaggatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg
    24241 agtgtggttt tgccgcaacc attcgcgcca agcagacaac aaattgttcc ttgcgacagg
    24301 gtgaaggaca cgtcgcgtag cactggatgg cgggagtaac ccagagacgc atgacgaacg
    24361 gcaagcaaag tcactggcga cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa
    24421 caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgc gccagcgtat
    24481 cggtaagcag gagcagaata gcaccagtac acattgccat aggcaacagc tgctgatggt
    24541 tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag accgatccaa ccaattatgc
    24601 ccgctattgc taccgtgctg gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca
    24661 gacgcgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca
    24721 tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc
    24781 agagatcgcg taaggtcacg gtggacagtc cccccagcaa ccagaaggta attgacggta
    24841 actgggtgta aggatcggcc agcgttttaa ttagcgatat ccccgctccg cacaacgttc
    24901 ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg
    24961 taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt
    25021 aaacaatggg taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga
    25081 cgcctaaaat atccggtgaa actaagggat tacgcaacat tccctgatag gtggttccgg
    25141 ctcctgccag cgctgcgccc agtaacagcg cagcaagaat acgcggcacg cggatttgcc
    25201 agaagacaat ttgttcctgc acagactcat tttcttgacg aagaattagc gccagcagtt
    25261 gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca
    25321 gcagcaaaat acatccgttt acgattctca ttagctcgcc accaattttt gatagtcggc
    25381 atcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg
    25441 gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat
    25501 tcccggcggg gcatcgagcc agccaaaggg caagccactt aaaaataaga tgcgttgttc
    25561 cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc
    25621 ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt
    25681 caggccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc
    25741 ggtatgcaac gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa
    25801 gcggaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agtggctggc
    25861 gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa
    25921 ctgttctgct gattgcgcca gttttccgtt aagcaataac caggggattt gtgtctgttc
    25981 gctaacctgc cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat caacgaccaa
    26041 atcgggatgt aacgccataa gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg
    26101 tcccagcctg ggtaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa
    26161 atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaaga gcaaatcggc
    26221 gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagag attgcgaagc
    26281 aaaacgatta actgtttgtg aaaaggacgg tagagactgc aggagcaggg tagatgcaag
    26341 tgcctgcgca aacgtccgtc tggttatggg catggtgaac atcctgaaaa gtaaaatcga
    26401 tatatacttt catatatatc gataaaaatc atgttcaata tcaaagttta gctattgaaa
    26461 acatgaagat gtgacgactt ccgcaagcct gttgaccctc tctatcatta aaaatgtatt
    26521 tatcaggtgg ctaatattat ttaaatcatt taaggctaaa tcatttaaat atgaatgaca
    26581 ttattgtttt ttatcgtcca cggttacgcg acgttgaagt tacttgtttt ataatgagcg
    26641 atttttgata tctcgccgag aaaattatca gcattctctc gtttcagtgt cgtgaatata
    26701 ctgttaacgg aggtgaaaag aatgctgggg gttatacaaa acctatgttg ttgtcgtccg
    26761 gattcaccgc ttgctgatag taagcagcgt aaccagaagt taccgcaaca agactttgag
    26821 gattttaaga acgacgttat taatgtcatt aacaacaatg aagatattta ttttcttaga
    26881 gagagtaatt tactgaatgc gtttattcgg acgtgcaaaa aaagagatga taacagttac
    26941 ggaaatatga tatttacgct ttattttgat aacgatatct atgggaacag aattatcaaa
    27001 gcatcgctga acggcggaga gttgcagtta ttaagcacag ggtgtagcaa tgataacatt
    27061 ctc
//
LOCUS       DAECOD010000053        25707 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.053, whole genome shotgun sequence.
ACCESSION   DAECOD010000053 DAECOD010000000
VERSION     DAECOD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.053"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            262..621
                     /locus_tag="KKO15_002237"
     CDS             262..621
                     /locus_tag="KKO15_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBD6361012.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(741..920)
                     /locus_tag="KKO15_002238"
     CDS             complement(741..920)
                     /locus_tag="KKO15_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBD6361013.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            994..2355
                     /gene="pabB"
                     /locus_tag="KKO15_002239"
     CDS             994..2355
                     /gene="pabB"
                     /locus_tag="KKO15_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBD6361014.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2359..2937
                     /locus_tag="KKO15_002240"
     CDS             2359..2937
                     /locus_tag="KKO15_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBD6361015.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            3121..4485
                     /gene="sdaA"
                     /locus_tag="KKO15_002241"
     CDS             3121..4485
                     /gene="sdaA"
                     /locus_tag="KKO15_002241"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD6361016.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4616..6214
                     /locus_tag="KKO15_002242"
     CDS             4616..6214
                     /locus_tag="KKO15_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6361017.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLFPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6218..7768)
                     /gene="yoaE"
                     /locus_tag="KKO15_002243"
     CDS             complement(6218..7768)
                     /gene="yoaE"
                     /locus_tag="KKO15_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBD6361018.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8231..9202
                     /gene="manX"
                     /locus_tag="KKO15_002244"
     CDS             8231..9202
                     /gene="manX"
                     /locus_tag="KKO15_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBD6361019.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9265..9495
                     /gene="manY"
                     /locus_tag="KKO15_002245"
                     /pseudo
     CDS             9265..9495
                     /gene="manY"
                     /locus_tag="KKO15_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
     gene            complement(9487..10260)
                     /gene="rlmA"
                     /locus_tag="KKO15_002246"
                     /pseudo
     CDS             complement(9487..10260)
                     /gene="rlmA"
                     /locus_tag="KKO15_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
     gene            complement(10426..10635)
                     /gene="cspE"
                     /locus_tag="KKO15_002247"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(10426..10635)
                     /gene="cspE"
                     /locus_tag="KKO15_002247"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD6361020.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(10648..10791)
                     /locus_tag="KKO15_002248"
     CDS             complement(10648..10791)
                     /locus_tag="KKO15_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBD6361021.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(11461..11748)
                     /locus_tag="KKO15_002249"
     CDS             complement(11461..11748)
                     /locus_tag="KKO15_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBD6361022.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(11823..11966)
                     /gene="mgrB"
                     /locus_tag="KKO15_002250"
     CDS             complement(11823..11966)
                     /gene="mgrB"
                     /locus_tag="KKO15_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBD6361023.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12125..12364
                     /locus_tag="KKO15_002251"
     CDS             12125..12364
                     /locus_tag="KKO15_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361024.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(12507..13298)
                     /gene="kdgR"
                     /locus_tag="KKO15_002252"
     CDS             complement(12507..13298)
                     /gene="kdgR"
                     /locus_tag="KKO15_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBD6361025.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            13475..14848
                     /locus_tag="KKO15_002253"
     CDS             13475..14848
                     /locus_tag="KKO15_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361026.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(14894..15775)
                     /gene="htpX"
                     /locus_tag="KKO15_002254"
     CDS             complement(14894..15775)
                     /gene="htpX"
                     /locus_tag="KKO15_002254"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBD6361027.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(15967..18015)
                     /gene="prc"
                     /locus_tag="KKO15_002255"
     CDS             complement(15967..18015)
                     /gene="prc"
                     /locus_tag="KKO15_002255"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBD6361028.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(18035..18733)
                     /gene="proQ"
                     /locus_tag="KKO15_002256"
     CDS             complement(18035..18733)
                     /gene="proQ"
                     /locus_tag="KKO15_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBD6361029.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(18830..19327)
                     /gene="msrC"
                     /locus_tag="KKO15_002257"
     CDS             complement(18830..19327)
                     /gene="msrC"
                     /locus_tag="KKO15_002257"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBD6361030.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            19457..20740
                     /gene="yebS"
                     /locus_tag="KKO15_002258"
                     /pseudo
     CDS             19457..20740
                     /gene="yebS"
                     /locus_tag="KKO15_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            20709..23329
                     /gene="yebT"
                     /locus_tag="KKO15_002259"
                     /pseudo
     CDS             20709..23329
                     /gene="yebT"
                     /locus_tag="KKO15_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            23409..24848
                     /gene="rsmF"
                     /locus_tag="KKO15_002260"
     CDS             23409..24848
                     /gene="rsmF"
                     /locus_tag="KKO15_002260"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBD6361031.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            24966..25202
                     /locus_tag="KKO15_002261"
     CDS             24966..25202
                     /locus_tag="KKO15_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBD6361032.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            25307..25498
                     /locus_tag="KKO15_002262"
     CDS             25307..25498
                     /locus_tag="KKO15_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBD6361033.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(25523..>25707)
                     /locus_tag="KKO15_002263"
     CDS             complement(25523..>25707)
                     /locus_tag="KKO15_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD6361034.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         6334 a         6516 c         6270 g         6587 t
ORIGIN      
        1 gctaattcat tatttatatt tataatttca attttatcta taaaactaac tatataaata
       61 aacataacca tgccgttgtg ttcgcccttt gattagcaac tctggttttc tttcttagct
      121 gaacaatctg gttatagcgt gcgggctgac cgttaatttt ctccatctta ctggataaca
      181 caccatgagc tgctaccttt aaaacacctg aatgtcaaca ggttaactcg tgccatatcg
      241 ttataatccc tttgatatta gatgcaaatt aaggtcatat atagccttat cgataacatg
      301 gttaatttta aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt
      361 gccatggatg tatcagcgcc aacaccaagt tcgatgaatg aaagcacggc gaagggaatc
      421 tttaaatatt taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct
      481 gaccaggaag gctggaaccc tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg
      541 gagacaggtg aacgctctgt gattaaaaat cctgaatact tttcaacata tatgcaggaa
      601 gaactaaaag cactggtctg agttaaattt atatcagcat aaatgggtca ggacgctttt
      661 aatcacatat aaaaagcgtc ctttttctca caatcaaatg atcatcggct tctataacga
      721 aatcgatacg gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattagctcg
      781 ccggagtggt tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctactcatc
      841 ccttgtgcca tcagttcctg gatccgctcg acagcttttt gctgctgttc atggatgagt
      901 gaaggtaaac ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg
      961 ctacccggca catagccagt agagtcagga ctgatgaaga cgttatctcc cactgtgatt
     1021 actttaccct ggcgtcagga cgccgctgaa ttttatttct cccgcttaag ccacctgccg
     1081 tgggcgatgc ttttacactc cggctatgcc gatcatccgt atagccgctt tgatattgtg
     1141 gtcgccgatc cgatttgcac tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc
     1201 gaaaaacgca caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc
     1261 gcagacattc gcccaacgca taacgaagat ttgccatttc agggcggcgc actggggttg
     1321 tttggctacg atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc
     1381 gttctgccgg atatggcagt gggtatctac gattgggcgc tcattgtcga ccaccagcgt
     1441 catacagttt ctttgctgag tcataatgat gtcaatgccc gtcgggcctg gctggaaagc
     1501 cagcaattct cgccgcagga agatttcacg ctcacttccg actggcaatc caatatgacc
     1561 cacgagcagt acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc
     1621 tatcaggtga atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca
     1681 ttccttcagc ttaatcaggc caaccgcgcg ccatttagcg cttttttacg tcttgaacag
     1741 ggtgcaattt taagcctttc gccagagcgg tttattcttt gtgataatag tgaaatccag
     1801 acccgcccga ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa
     1861 gcagaaaaac tggcgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta
     1921 atgcgtaatg atatcggtcg tgttgccgta gccggttcgg taaaagtacc agagctcttc
     1981 gtggtggaac ccttccctgc cgtgcatcat ctggtcagca ctataacggc gcgactacca
     2041 gaacagttac acgccagcga tctgctgcgc gcagcttttc ctggtggctc aataaccggg
     2101 gctccgaaag tacgggctat ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc
     2161 tggtgcggca gcattggcta tttgagcttt tgcggcaaca tggataccag cattactatc
     2221 cgcacgctga ctgccattaa cggacaaata tactgctctg cgggcggtgg aattgtcgcc
     2281 gatagccagg aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc
     2341 caactggaga agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatctcgc
     2401 tttcaacttt tgcgcccgca aattaaccgg gaacccctaa atcatcgtca ggctgccgtg
     2461 ttaatcccca tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg ttcaattcat
     2521 ctgcgtaaac acgctggaca agtggcattc cctggaggtg cagtcgatga cacggacgca
     2581 tcagttatcg ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc
     2641 gttgaagtta tcggcgtgct gccgcccgtc gatagcgtaa ctggctatca agtaacccca
     2701 gtggtcggca ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggcg
     2761 gtgtttgaaa tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc
     2821 taccgccgtg gcgattcaca tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg
     2881 ggaatgaccg caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa cccctgacta
     2941 tacttatctt tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc
     3001 gcggtttttg attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta
     3061 cactatgcgc tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc
     3121 gtgattagtc tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta
     3181 gggcctatga aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat
     3241 agcgttactc gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac
     3301 cacaccgata tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc
     3361 gacagtattc ccggttttat tcgcgacgta aaagagcgcg aacgtctgct gctggcacag
     3421 ggacggcatg aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg
     3481 ccgctgcatg aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc
     3541 aaaacttatt attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat
     3601 gctgccaacg aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac
     3661 tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac
     3721 agcaagaaag agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc
     3781 gatcgcggga tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg
     3841 tctgccctgc gccggatgtt agtttccagc gataaactgt ctaacgatcc gatgaatgtc
     3901 attgactggg taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt
     3961 gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct ggcttactat
     4021 gaccacttta ttgaatcggt cagcccagac atctataccc gttactttat ggcagcgggc
     4081 gcgattggtg cattgtataa aatgaacgcc tctatttccg gtgcggaagt tggttgccag
     4141 ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact gctgggcggt
     4201 agcccggaac aggtttgcgt ggcggcggaa attggcatgg aacacaacct tggtttaacc
     4261 tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct
     4321 gtgaaggcga ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg
     4381 ctggataagg tcatcgaaac gatgtacgaa accggtaagg acatgaacgc caaataccgc
     4441 gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg actaatactt cttactcgcc
     4501 catctgcaac ggatgggcga atttataccc gctttctcgt ctgctgtaat attccccact
     4561 acacttccac tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca
     4621 aaaagcacaa cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg
     4681 cgcactcgtt acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa
     4741 tcaacaacgg gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgtt
     4801 tcccctacag gcaggtagca aagtcttgct tccgctgatt ggtctgccct gctctgtcgc
     4861 ccatttgcca ttacgtaaac aggcggcaaa actccaaact gtgcgatcca ttggcctggt
     4921 gcaagacggc acactttatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga
     4981 cattctggct gaacttcctg caccgcaacc acttttacgc ctgacgatcg accgtgccct
     5041 gattaaaggc agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg
     5101 ggtcatagag atgattaaca tcgacttact gacggcaatg ctccttgagc cacaactgcc
     5161 gcaaatcagt agcgccagcc tgacggtgga caaacggcat ttgctctatg gtaatgggct
     5221 ggtagattcc cttccgcaac ctgaagacaa tgaaaactat caggtttctt cgcaaagctt
     5281 tccttttacc attaacgtaa atggtccggg ggctacggcg ctggcatggc actatcttcc
     5341 aacacaatta ccgctggcgg tgctgctaag tttactggtg ggctatatcg cctggctggc
     5401 gaccgcttac cggatgagct tttcccgcga aattaatctg ggcctggcgc aacatgagtt
     5461 cgaattgttc tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat
     5521 tttgctgcgc tggaataatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat
     5581 cgcggaagaa catcatttaa ttgtgccact gacccactat gtgatggcag aaaccattcg
     5641 tcagcaccat gttttcccga tgagtagtca gtttcatgtt ggcattaacg tcgcacccag
     5701 ccattttcgc cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc
     5761 gattcagcaa ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattatcg
     5821 gattgcccgc gagctgcatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg
     5881 caacagctcg ttttcctggc ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc
     5941 atttaccgca gctataggtt ctgacgcggt taactcgacg gtgaccgata tcatcatcgc
     6001 gctggggcaa agactgaata ttgaactggt ggcggagggc gtggaaacgc aagaacaggc
     6061 gaagtatttg cgccgtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat
     6121 gccgctacgt gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa
     6181 tggacatatc acgcccatta tgtcgttacg ttaacgctta ctcatcttca tcgtgcgccg
     6241 gttgctcttt aacaatgcga accagatcaa cacgataatc attggcttca aggatggtga
     6301 tatgcagtgg ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga
     6361 tcacgaggcc cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg
     6421 cctgctgcaa ggcatgcaaa tctgtaccgc cttttaccag ccagccgtta ccatcggtaa
     6481 tgatttccgg cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca
     6541 gcggcgtgac caagccttgt accacaccaa actcgttggt cacgataaca aagctcccgc
     6601 gagcgcgacg cagcacgccc agcaggttga tcggatcgag ggtttccggg acgataatcg
     6661 ccggagacgc cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt
     6721 ctttagcacg tacgataccg atgatttcat ccagttcacc gcgacatacc gggaacagac
     6781 tgtgcggtga agagagcagt tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc
     6841 agcttatttc accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta
     6901 cgccgttaat catgtaccgt tcttcttcgg caaatgcacc ttccgggatc ggcatcgggt
     6961 tatcggcatc gtgctgaaca ttggcctgac gtttcccgcc catcaaacgc aggatggcat
     7021 cggcagtacg cgctcgcagc ggcaaagtcg actggtgacg aataaagttg cgacgcgcaa
     7081 tctggttaaa cacttcgata atgatcgaga agccaatcgc ggcatacagg taacctttcg
     7141 gaatgtggaa accgaaacct tctgccacca gactcagacc aatcattaac aggaagctca
     7201 gacagagcac caccaccgtg gggtgctggt taacgaatcg cgtcagcggt ttcgatgcca
     7261 gcaacataac cgccatcgca atcactaccg ccgccatcat caccggcaga tggttaacca
     7321 tccctactgc agtaattacc gcatccaacg agaagacggc gtcaaggatg acaatctgtg
     7381 tgacgaccac ccagaaactg gcgtagcctt taccgtggcc ggaatcatga tcgcggtttt
     7441 ccagccgttc atgcagttcg gttgttgctt tgaacagcaa gaatatcccc ccgaacaaca
     7501 taatcagatc gcgtccggag aaggagaaat ccatgacggt aaatagcggt ttggtcagcg
     7561 tgaccatcca tgaaatcagc gacagcagcc ccagacgcat aatcagcgcc agtgataacc
     7621 ccagcaaacg cgctttatcg cgttgttttg gcggcagttt gtcagcaaga atggcgatga
     7681 agaccaggtt atcgataccc agcacaattt cgagaacaac aagcgtgagt agccccgccc
     7741 aaattgaggg gtccattaag aattccatga caagctcctg cttaaggaat agctattcga
     7801 cgccagaaat aatgcaggcg taacgacaaa atgcaaacga aaggtgcggc atagagtgcc
     7861 agaaaggcag gcgttaaaag gcctgatgct gaaatgacgt cggtgacgat ccatactgcg
     7921 ggctactgcc ctatactcca tggttgttaa acgggagtta aacatatcag agacgcctct
     7981 gatttggcaa agatttacct tcctttgcaa acgaatgtga caaggatatt ttacctttcg
     8041 aaatttctga taatcgaaag ttaaattacg gatcttcatc acataaaata attttttcga
     8101 tatctaaaat aaatcgcgaa acacaggggt ttttggttgt agcccttatc tgaatcgatt
     8161 cgattgtgga cgacgattca aaaatacatc tggcacgttg aggtgttaac gataataaag
     8221 gaggtagcaa gtgaccattg ctattgttat aggcacacat ggttgggctg cagagcagtt
     8281 gcttaaaacg gcagaaatgc tgttaggcga gcaggaaaac gtcggctgga tcgatttcgt
     8341 tccaggtgaa aatgccgaaa cgctgattga aaaatacaac gctcagttgg caaaactcga
     8401 caccactaaa ggcgtgctgt ttctcgttga tacatgggga ggcagcccgt tcaatgctgc
     8461 cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt gcaggcgtta acattccaat
     8521 gctcgtggaa acgttaatgg cccgtgatga tgacccgagc tttgatgaac tggttgcgct
     8581 ggcagtagaa acaggccgtg aaggcgtgaa agcactgaaa gccaaaccgg ttgaaaaagc
     8641 cgcgccagca cccgctgccg cagcaccaaa agcggctcca actccggcaa aaccaatggg
     8701 gccaaacgac tacatggtta ttggccttgc gcgtatcgac gaccgtctga ttcacggtca
     8761 ggtcgccacc cgctggacca aagaaaccaa tgtctcccgt attattgttg ttagtgatga
     8821 agtggctgcg gataccgttc gtaagacact gctcacccag gttgcacctc cgggcgtaac
     8881 agcacacgta gttgatgttg ccaaaatgat tcgcgtctac aacaacccga aatatgctgg
     8941 cgaacgcgta atgctgttat ttaccaaccc aacagatgta gagcgtctcg ttgaaggcgg
     9001 cgtgaaaatc acctctgtta acgtcggtgg tatggcattc cgtcagggta aaacccaggt
     9061 gaataacgcg gtttcggttg atgaaaaaga tatcgaggcg ttcaaaaaac tgaatgcacg
     9121 cggtattgag ctggaagtcc gtaaggtttc caccgatccg aaactgaaaa tgatggatct
     9181 gatcagcaaa atcgataagt aacgtattgt gttgattatc attcagtttt cacacttaag
     9241 tcttacgtaa acaggagaag tacaatggag attaccactc ttcaaattgt gctggtattt
     9301 atcgtagcct gtatcgcagg tatgggatca atcctcgatg aatttcagtt tcaccgtccg
     9361 ctaatcgcgt gtaccctggt gggtatcgtt cttggggata tgaaaaccgg tattattatc
     9421 ggtggtacgc tggaaatgat cgcgctgggc tggatgaaca tcggtgctgc agttgcgcct
     9481 gacgccgctc tggcagtcga acacttcttt tgctgccagt gtttgccaga cttctggctt
     9541 cgcacgccag gcaaacggcg tcatctgcaa taatgcgacg gcttcatcac cgcgaagacg
     9601 catcggataa cacaactccg cactctgctg taatgtaaaa ccttccagtt gttctgcatg
     9661 aggtgcatga agatgtactt cattgtaaat cagccccttc agctccatca aatgtcgcgg
     9721 tcccggcgtg gcagtaatga cccagccgcc gggcttcact actcgtgcta attcttctgc
     9781 tttacacggc gcgtaaatac gtattatggc gtccatactg gtatcggaaa acggcaaacg
     9841 gtggctggaa gcgacacaaa gagtgacctg cggatagcgt ttcgccgccg cttttatcgc
     9901 taccttcgag acatccaggc caaacgtggt gatttcgggc aacgcatcgg caaatgcgtg
     9961 tgtgtaatac ccttcaccac agccaatatc cagcaccgcc gtggcctttt catcaagccg
    10021 ttcccgcagt tgggcgacaa ttgcatcacg cagcggctga taatgtccgg catctaagaa
    10081 tgcgcggcgt gcttgcatca tttccgcgct gtcgcccgga tcacgagacc gtttatgctg
    10141 aacgggcagc agattgacat acccttcttt cgccatatca aactgatgtc gttggggaca
    10201 gatatagctg tttttttcac gcgaaagagg ctgatggcaa agtggacagg aaaacgacat
    10261 gacaactccg gcaggatact aaaggccgca agtgtaacgc gaatcacgcg gcgggcaaat
    10321 atccgaacgc caggagcgaa gataaagcat agtaaaaagc ctcgcattcg acgaggcttt
    10381 atagcgagat tgaagcgtat tcacacttca gatcagtgga ttcgatcaga tagctgttac
    10441 gttaacagct gccggacctt tctggccgtc ctgaatttcg aactcaacgt tctgaccttc
    10501 agccagagtt ttgaagccat taccctggat agcggagaag tgtacgaaca catctttgct
    10561 gccatcagcc ggagtaatga agccaaaacc tttagactcg ttgaaccact taacctgacc
    10621 tttaatcttt gccatttgaa aaattcctta gattgttttc ttcgcccgca ggcataacat
    10681 agataaaact gacacattac tgcatgaggc accaatataa ggctcggcag agaagcggta
    10741 ttcaacgtca acgtgtttac tcaggacttc tttactgaaa atgccacaca taaacagaac
    10801 tgtacctcgt ttaacccgaa atcttttatc acatacaacg ttaattatgg caagccattt
    10861 ttaaacatgt ctcgatcaga cgcacaaatc tcgatacgtc ttccactttt tttgcacact
    10921 tatgcaacgg aatacgcacc gatatatcat tgtgcttaac cttgccagtt caggcagata
    10981 cttaaactgg cgtagtttct aacatagttc aataagttca gttgttccag gccagaggga
    11041 gaagttatta catagtgcgt gcaatatcac attttctgct atgcaatgaa taaaaagtta
    11101 tatcactttt tctcataaaa cagtcagtta acggctatca attaccctaa agagaagtca
    11161 atccccaaag ggattgtaaa tttaaaataa gaaaaattga tgagtgagca aaaaaatcaa
    11221 gagagaaacg tttcgctaat aaataatacc acgcttaatc acaaatctga tgtaaacatc
    11281 ctaataaatt aatgaggaaa atatcttttt ctgatatccc gttacgcaaa taaataatat
    11341 gataattatt tgaagtcaga ccagagtttt aatttgaagc agttgcacca tgacagtgcg
    11401 tataatcagg atgaattgag taacccttca acgacggggt tactcaagat tgaggaagga
    11461 ttatcgttcg gcgaccaggc gaataatatc ttcgtcatca accgattttt gactctcaat
    11521 ggtcttttta tctgctttct ctggttcgtt tgcttcgcac aaacgacgta acagtgcatt
    11581 ttgacgtttt tgctgatcca gcaacgcctc aagcagttca atctgttcgt tagtccgtga
    11641 actggcacga ttgataaaaa accacaagat gagcccgata agaagaacca ccaccgatac
    11701 aaccaaagac gcaatgttca tcacgcctga atttacaact tcgttcattt caccacctct
    11761 gagtagaggc gctattctac cactgctgga gaggaagaaa atctagtgct gaaaaaatga
    11821 tatcaccacg ggataaactg gttaatggca caaattccgc tgaaaaattg tacatcctga
    11881 tcgcacatca tgttgaatac ctgcgcccaa agcagcaagc aagccaacac cacgacaacc
    11941 agaacgaccc atcgaaactt tttcactcca atctccgctt cacctacctt atgtcattaa
    12001 actagcatgc gttagttaaa cagcgcctaa ctatgtcgat attcgtgctt tttcggaatg
    12061 agtagcttgc ttcagcgcat attgccgcta cgattaagcg aacataaaaa gagaagagat
    12121 tgtaatgcga ttcatcattc gcacagttat gctgatagca ctggtatgga taggattatt
    12181 actcagcgga tatggcgttt tgattggaag caaagagaat gccgcaggat taggtttgca
    12241 atgtacctat ctgactgcca gaggcaccag cacggtacaa tacttgcaca ctaaaagtgg
    12301 tttcttggag ataacagatt gcccactgtt acgtaaaagc aatatcgtgg tcgataatgg
    12361 ttaagggtaa taaataacca gattctcact catgagtaaa atattcttat agcaagcgcg
    12421 tttaatataa cgcaccttca actttatttt tctcatgcta aataaagaac ctgtctgttt
    12481 gatattccga caggttctta ctctcatcag aacggatagt cgtgataacc catttgggca
    12541 gaaattttgc gcgctgcggt atgcaacatt gcgacatatt cctgtaaacg ctcttcagag
    12601 aaacgcaacg tcgggaagga gatgctcaaa ccggcaatga ccacgccaaa gcgatcaaat
    12661 accggtaccg caatgcattg cagcccttct tcctgctctt cgttatcttc tccatacccc
    12721 tgctcgcgca cctggtccag aacgggtaat aacgcttctg tactggtgat ggtccgctcg
    12781 gtactgcgtt tatactccac gccctcaaga atttgcttca cttcatcgcg atcgcgccat
    12841 gccagcagta ccttaccaat cgcggtgctg tacagcggat tacgacgccc aatccgtgaa
    12901 tacatgcgca aattgtacat agagtcaatt ttgtgaatgt aaacaatact gtcttcgtcc
    12961 agtgcgccga ggtggatagt ttctttggtc aggcgggaga tctcacgcat ctggatatct
    13021 gcgctacgaa ttaaatcgac gttttgtaac gcgcgagcgc ccagttcaaa caatttcagg
    13081 gtcagggaat atttctccga ctccccttcc tgcgccacat aacctaaggt tttcatggtc
    13141 tgtaaaaagc gataaacggt gctttttgac atcatgacgc gctgcgacag ctcggttatc
    13201 cctatttcgc gctcttcacc cagcgcctgc aaaatgccaa aaacttttag cacggaagat
    13261 acagaatcag gctgtttatc cagatctgcg ttagccattt atcacctcat tgcgagtgtt
    13321 ttataaaaat cagaactgtt ttttattata atttcgcacc agggtggtcg caatccatct
    13381 tttgccggtt agttacaatt ctgcgacatc caccgtgaat atcagtgcta gaatcatacc
    13441 cctgttgatt attcaccaaa gatataaaat tcctatgcca aaagttcagg ccgacggcct
    13501 gccattgccc cagcgatacg gtgcgatatt aaccattgtg attggtattt cgatggctgt
    13561 ccttgacggc gcaatcgcca acgtcgccct gccaacaatt gccacggacc ttcatgccac
    13621 accagccagt tccatctggg tagtgaacgc ctatcaaatc gccattgtca tctccctgct
    13681 ctcgttttcg tttctgggcg atatgtttgg ctatcgacgt atttataaat gcggtctggt
    13741 cgtttttctg ttgtcttcac tgttctgcgc cctttctgat tcgctgcaaa tgctcaccct
    13801 tgcgcgtgtc atacaaggtt tcggcggtgc agcgttgatg agcgttaata ccgcacttat
    13861 ccgcctgatc tatccacaac gttttctggg gagagggatg ggcataaact cgtttattgt
    13921 tgccgtctct tctgctgccg ggccgacaat tgctgcagca atcctctcca tcgcatcctg
    13981 gaaatggtta tttttaatca acgtaccgtt aggtattatc gccctgcttc tggcgatgcg
    14041 ttttctgcca cccaatggtt ctcgcgccag taaaccccgt ttcgacctgc ccagcgccgt
    14101 gatgaacgcg ttaaccttcg gcctgcttat cactgcgttg agtggtttcg ctcaggggca
    14161 atcgctgacg ttaattgctg cggaactggt ggtaatggtt gttgttggta ttttctttat
    14221 tcgccgccag ctttctcttc ccgtaccgct gctaccggtg gatttactgc gtatcccgct
    14281 gttttcactt tctatttgca catctgtttg ctctttctgc gcacaaatgc tggcaatggt
    14341 ttccctgccc ttttacctgc aaaccgtgct cgggcgtagt gaagtcgaaa caggtttact
    14401 tctgacaccg tggccgttag caacgatggt gatggctccg ctggcaggct atttgattga
    14461 acgcgtacat gcaggattgc tgggtgcttt agggttgttc atcatggcta cggggctttt
    14521 ttccctggtt ctgctgcccg cgtcacctgc ggatatcaat attatctggc cgatgatctt
    14581 atgtggcgct ggatttggct tattccagtc acccaataac cacaccatta ttacctccgc
    14641 tccgcgcgaa cgtagcggtg gggccagtgg catgttagga acggctcgtc tactgggtca
    14701 gagtagtggc gcggcgctgg tggcgctgat gctaaatcag tttggtgata atggtacgca
    14761 cgtctcgctg atggctgcgg ctattctggc agtgattgct gcctgtgtca gtggtttacg
    14821 tatcactcag ccacgagcca gggcataaaa aaagcgcgtc gatcaggacg cgctttttag
    14881 tatttacttc atattacttc aggtattcac ccgtacgcag agcttcaata cgtttatcca
    14941 gcggcgggtg agtcatgaac aactcactga gcgatttcga cttaccgtta atgcagaaag
    15001 ccatcatgct ggttgcttct tgcggttcat agctggtttt caggcgctgc agcgcggcaa
    15061 tcattttctc gcgaccaacc agttttgccg aaccggcatc agcatggaat tcacgatgac
    15121 gcgagaacca catggtgata atgctcgcca gaataccaaa caccagttcc agaaccgttg
    15181 caaccgcaaa gtagatcagc gggttgccgt tgctctcttc accttcatca cgatttccgc
    15241 ccataaaacc cgcggcaagc tgcgccagaa tacgggaaat aaagataacg aaggtgttca
    15301 ccacgccctg aatcagcgtc atggtgacca tatcaccatt ggcgatgtgg ctgatttcgt
    15361 gagcaattac cgcctcggct tcatccgggc tcatgttctg cagcaaaccg gtgctgacag
    15421 caaccagaga ggcatcacgg cgcgcaccgg ttgcaaaagc gttgatgtcc ggcgcatggt
    15481 agatagccac ttgcggcata gcgatccccg cctgacgagc ctgggttgct acagtattga
    15541 ccagccaacg ttccctttcg ttacgcggtt gctcgatcac ttccccgcca acagatcgta
    15601 atgccatcca tttggacatc agaagcgaaa cgaaggaacc accaaaaccg aacagcaagg
    15661 ccatgatcat cagcccctga acgctgctcg actgtatccc tgtcaggctc agtaccagcc
    15721 cgaaaacgac cattacggcc aggttcgtta gcaggaagag cgcgattcgc atcataattt
    15781 tctttttacc tcagtttaac aaaacgcaat atgcgatacc cacatcgtat gggttacgcg
    15841 actattttca agtctggata gtgcgtaagt caccagaaag acacaacttt acattttgta
    15901 gcatctgatt tacggcatct tgccgctgtt aaaaaatcag gcacaatttc ttgtgcctga
    15961 ttgatattac ttgacgggag cgggttgttc cgcgggtctg gctttttcaa gcttcgccag
    16021 atcgagtgcg atattcaccg tctcatccag ataaggatcc ggctcctggt aatctttcga
    16081 tagatcatcc agcttcttca actccggttt accttctcgt ttaaagcgtt cgttcaaacg
    16141 cgccagacgc gtcgcatcat cttcattatt ctctttctca cgcacagcgt agttcagaga
    16201 aacgatattg cgcttgtcct tcatagcgtt gaagcgcgcg atatccttca tgatgttctg
    16261 gaactcagga tctttcgcga tacgcgcatt atgttccttc agcagctccg gttcaaaggc
    16321 agttaaatct cctgatttca cataagtcgc ggcatcgatg ctatcccacg gcagcgcgtt
    16381 atcttcgaat ttctcacccg tttccgtttc ttcattaccc gtcggcatga tgatgtctgg
    16441 tgttacgcct ttacgttgcg tactgccgcc gttaacgcga tagaatttct ggatcgtgta
    16501 ctgcacagaa cccagcgctg gccattcagg acgtaacatc tgatcgtaaa tacggttcaa
    16561 tgaacggtat tgctgaacgg tgcctttacc aaacgtcggt tcacccacaa ccagcgcacg
    16621 accgtaatcc tgcattgccg cggcaaagat ttctgaagcc gaagcactga aacggtcaac
    16681 cagtaccacc agcgggcctt tatagaacac ctgcccgtcg gtatcgctgt cttcacgaac
    16741 cttgccgttg ttatcgcgga cctgaacaat gggacccgca ggaataaaca gacctgagag
    16801 cgataccgct tcggtcaacg ccccaccgcc attgctacgc aggtcgatga tcacactgct
    16861 gacattctgt ttttccagtt tctgcagttg aactttgaca tcgtctgtca aacccacata
    16921 gaagcccgga atatccagca cgccgacttt ctctttaccg acggtcttca ccgacatttt
    16981 aaccgcgcgg tcttcgagac gaatacgttc acgggtcaac gttacagtac gggtcttggt
    17041 ccctttacca gcaggtaaaa tttccagacg aactttactg cccttcggcc ctttaattaa
    17101 ggcaaccaca tcatcaagac gccagccaat cacatcaacc atcggcttgc ctgtttgacc
    17161 aacaccgaca attttgtcac caacgctgat agctttactc ttcgctgccg gaccacctgc
    17221 caccatcgaa ttgataacgg tgtagtcatc atccatttgc agcactgcgc caataccttc
    17281 cagtgacaaa ctcatttcag tgttgaactg ttcggtatta cgcggggaaa gatagttggt
    17341 atgcggatcg atttcacgcg caaacgccgt cattgccagc gagaaaacat cttcgctgtt
    17401 ggtttgcgcc agacgacgaa tggcaaattt gtagcggcgt gtcagggttt cacgaatttc
    17461 tttatccgtt tttcctgtca gcttcaggct taactcgtcg aatttgactt tactgtccca
    17521 ctgcgcgttc aactcagcct cgtttttcgg ccagggcgct ttgctgcggt caaggttata
    17581 agtgtcgttg ccggtgaaat ccatcggctt ttccagtacc gacaaagcgt actggtaacg
    17641 ttcaaaacgg cgcttttgcg ccagattgta gagatcgtag aaaacgtcga gtttgcctga
    17701 acgcagttca tcgcctaact cggttttctt tttcgcgaac tgttcaacat cgcttgccag
    17761 cagcacattg tggctgtaat cgagcagatt caggtagcgg tcaaagattt tggccgaaaa
    17821 tgcctgatcg aggtcgaact ggcgataatg agaacgggtg aagcgcgacg ttacgcgctc
    17881 acttaccgtc gcatgctgcg tctcttcctt taataccgga atttgatcag cacgcgtgat
    17941 atcttctaca gcgaaggtct ggcctgctat tgcaagcagg ccagctaacg cggtaagcct
    18001 aaaaaacatg ttcatgcctg gcccggcctc cgtttcagaa caccaggtgt tctgcgcgca
    18061 caatcaaaga catacccgaa ttcagctgga cgcggacgcc gtctttggtg atttctaata
    18121 cggtggcatc catcgcgttt tgacccgctt tcaccttcag ggcttgtccg acagtcagag
    18181 ctgaaatgtc agaaaccggg gtgtgctgtt cttcgcgagg tgcttttact gtttttggcg
    18241 ctttctctac cggcttttgc gcacgaggtt tacgttcagc gccttctttg cggcgtggcg
    18301 tggtcggacg tggcttgcgt tcgcggcgcg gtgcgtcttc tttctcacca gcagctgcgg
    18361 cagcttcgcg ttttttcgct tgctgttcag cacgctgtgc ctgaacacgc gctttcgctt
    18421 cttcaagctg cttgcgagca tgctctacat gttgctcgtc cagctcaccg catgggttgc
    18481 cgtcaagatc gacacgcgtt gcgccgggtt taacaccgta aagataacgc cagctcgaag
    18541 tgtagagacg taaagcggat cgtaattgcg ttttgctcag gttcatttcc ccggcaacac
    18601 gatcgaccaa atcctgaaaa ataccaattt tcagcggacg cgcttcacct tccgcactga
    18661 aacagtgggg aaaacgttcg gccagaaacg cgattacttc tttactgcta ttcaacttag
    18721 gttgattttc catgaaattt cctgattaca acggacgtag ccaacaagcg caggcatgaa
    18781 caggcgtcat tataatgacg ctaccagtaa atgctacgtt atccgttgat tatcctgcga
    18841 cgctcgcaaa gaattttttg taatccgtcg ttgcaagcac tttttcaagc tgtgccacaa
    18901 gctgacgtaa gccttgctcg tcctcgtctg taaagcgagc gaagacggta ctatcgatgt
    18961 cgagaacacc aataatctga tttttcacca ccagcggcag aacaatttca gaattactcg
    19021 ccgcatcaca ggcaatatgc ccgtcaaacg cgtgaacatc ctcgatacgc tgcacttgat
    19081 tgcgggcaac cgctgtgccg cacaccccgc gcccgacggg tatccggaca caggcaattt
    19141 tgccctgaaa tggtccgagt accagtgtat cgtcctcaag caaataaaaa cctgcccagt
    19201 taacgtcagt gagacgctcg tataacaacg cactggtgtt cgcaagcgtt gccagaaaac
    19261 tggtttctcc cgccatcagc gcgttaaagt cgcgatttaa atccgcgtaa aattctgttt
    19321 tgttcattac ttaatcactt ggttgtctta cctggaactg ccagcctatt aaaataagca
    19381 ttaaatgcgt taatgctcaa gatcattccc atcatgggtt aagattaatg ttaattctta
    19441 ttacatttgg cacgtcatgg ctcttaacac accacaaatt acgccgacaa aaaagataac
    19501 agtgagggca atcggcgagg aactgccgcg tggtgattac caacgttgcc cgcaatgtga
    19561 catgctgttt agcctgcccg agataaattc tcatcaaagt gcctattgtc cgcgctgtca
    19621 ggcaaaaatt cgcgacgggc gcgactggtc gctaacgcgc ctggcggcaa tggccttcac
    19681 catgctgttg cttatgccgt ttgcctgggg cgaaccgctg ttgcatatct ggctgttagg
    19741 cattcgtatt gacgccaacg tcatgcaagg catctggcaa atgaccaaac agggcgatgc
    19801 gataacggga tcaatggtct ttttctgcgt tattggtgcc cccctcattc tggtgtcctc
    19861 catagcatat ttatggtttg gtaaccgact gggaatgaat ttacgtccgg tactgctgat
    19921 gcttgagcga cttaaagagt gggtaatgct ggatatctac ctggtcggca ttggcgttgc
    19981 ttctataaag gtacaggatt atgcccatat ctaggcgggt gtcggcttgt tctcttttgt
    20041 ggcgttggtg attttaacga cggtgacgtt gtcacatctt aatgttgagg agctgtggga
    20101 gcgatattat ccgcagcgcc ccgctacgcg tagggacgag aaacttcgtg tctgtcttgg
    20161 gtgccatttt accggctatc ctgatcagcg tggtctctgc ccgcgttgcc atatcccgct
    20221 acgcctgcgt cgtcgtcata gtttgcaaaa atgctgggcg gcgctgttag cgtcaatcgt
    20281 tttgttgtta ccagccaacc tattgcctat ttctatcatt tatctgaatg gtggccggca
    20341 ggaagatacg attctttccg gaattatgtc gctggcaagt agcaacattg cggttgcggg
    20401 aatcgtgttt atcgccagta ttctggtacc gtttactaaa gtgatcgtca tgttcacttt
    20461 actgttgagc attcatttta aatgccagca aggtttacgc acacgcattc tgttactgcg
    20521 gatggtgacc tggattggac gctggtcgat gctcgacctg tttgtcatat ctttaaccat
    20581 gtcgctgatt aatcgcgatc agatcctcgc ttttactatg ggaccggctg cgttttattt
    20641 tggcgctgcc gtaattttga ctattcttgc tgtggaatgg ctggacagcc gcttactttg
    20701 ggatgcacat gagtcaggaa acgcccgctt cgacgactga agcgcagatt aaaaataaac
    20761 gccgtatctc acctttctgg ctgctgcctt tcatcgcgct aatgattgcc ggttggctga
    20821 tttgggacag ttatcaggac cggggtaata ccgtcaccat cgactttatg tcggcggatg
    20881 gtattgttcc gggccgtacg cctgttcgtt atcagggcgt tgaagtcgga acagtgcagg
    20941 atatcagcct cagcgacgat cttcgtaaga ttgaagtcaa ggtcagcatc aagtccgata
    21001 tgaaagatgc gctgcgcgaa gagactcagt tctggctgat gacgccaaaa gcatcgttgg
    21061 caggtgtctc cgggctggac gccctcgtcg gtgggaacta tatcggcatg atgccgggta
    21121 aaggtaaaga gcaggatcac tttgtcgcgc tcgataccca accgaaatat cggctggaca
    21181 atggcgatct gatgatccac ctgcaagccc ccgctctcgg ttcgctgaac agcggttcat
    21241 tggtctattt ccgcaagatc ccggtgggaa aagtctacga ctatgccatc aatcccaaca
    21301 agcaaggcgt ggtgattgat gtcctgatcg agcggcgttt taccgatctg gtgaaaaaag
    21361 gtagccgttt ctgaaacgtt tccggcgttg atgccaacgt cagtatcagt ggcgcgaagg
    21421 tgaaactgga aagtctggcg gcactggtta acggtgcgat tgccttcgat tcacctgaag
    21481 agtcgaaacc tgccgaggcg gaagatacct ttggtctgta tgaagatctg gcccacagcc
    21541 agcgtggagt aataataaaa ctggaactgc cgagtggagc aggattaacc gccgactcga
    21601 cgccgttaat gtatcagggg ctggaagtcg gacagctgac taaactggat ttaaatcctg
    21661 gtggtaaagt cactggggaa atgaccgttg atccctgctt cgtgaaaata cccgcatcga
    21721 attacgcaac ccgaaattat cccttagcga tgctaatctc agcgccctgc tgaccggaaa
    21781 aacctttgag ttggtgcccg gcgatggcga gccacgcaaa gagttcgttg ttgtgccggg
    21841 cgaaaaagcc ctgctgcaag agccggacgt tctgacactg accctgacag caccggaaag
    21901 ttacggtatt gatgcgggtc agccgctcat tcttcacggc gtgcaggtag gccaggttat
    21961 cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc gccatcgagc ctcagcatcg
    22021 agaactggta aaaggcgata gcaaatttgt cgtcaacagc cgtgtcgacg tgaaggtggg
    22081 gctggatggc gttgagtttc tcggtgccag cgcctcagaa tggattaacg gcgggatacg
    22141 tattctgccg ggcgataaag gcgagatgaa agccagctat ccactgtatg ccaatctgga
    22201 aaaagcgctg gagaacagcc ttagcgattt acccaccaca accgtgagtt tgagtgcaga
    22261 gacgctgccg gatgtgcagg caggatcggt agtgctgtac cgtaaatttg aagttggtga
    22321 agttattacc gtgcgtccgc gagctaacgc gttcgatatc gatctgcaca ttaaaccgga
    22381 atatcgcaac cttttgacca gcaacagcgt gttctgggca gaaggcgggg cgaaagttca
    22441 gctgaatggt agtggcctga ccgtacaggc atccccgctc tccagagcat taaagggggc
    22501 cattagcttc gataacctca gtggtgccag cgccagtcag cgtaaaggcg acaagcgaat
    22561 tctgtatgct tccgaaacag cggcccgtgc ggttggcagg cagattacgc ttcacgcttt
    22621 cgatgccgga aaactggcgg tcgggatgcc aattcgctat ctcggtattg atatcgggca
    22681 aatccagacg ctggatctga ttaccgcacg caatgaagta caggcaaagg cggtgctcta
    22741 tccggaatat gtccagacct ttgctcgcgg tggtacgcgc ttctcagtgg tcacaccgca
    22801 aatctcggca gctggcgttg agcatctaga tactatcctc cagccgtata tcaacgtcga
    22861 accaggccgt ggcaatcctc gtcgcgactt tgaattagaa gaggccacca ttactgattc
    22921 gcgttaccta gatggtttaa gcattattgt tgaagcgccg gaagccggtt cgttaggcat
    22981 cggtacgcct gtgctgttcc gtggtctgga agtaggtacg gttaccggaa tgacgctggg
    23041 gacattgtca gatcgcgtga tgattgcgat gcgcatcagt aaacgctatc aacacctggt
    23101 gcgtaacaat tccgtcttct ggttggcatc gggttacagt ctggactttg gtctgacggg
    23161 cggcgtagtg aaaaccagca cctttaacca atttatccgt ggcggcatcg ccttcgccac
    23221 gcctccgggt acgccacttg caccgaaagc ccaagaaggc aaacacttcc tgttgcagga
    23281 aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt cccaaataat gcccactgct
    23341 ccggcgtgcc tgcgccggag cgcttatgct aaactgcgcg cctgtttttt tgccagtggt
    23401 acatgctcgt ggcccaacac accgtttatt tcccggacgc gtttctgaca caaatgcgcg
    23461 aagctatgcc ttcgacgctc tcatttgatg attttcttgc cgcctgtcag cgcccgttgc
    23521 gccgcagcat tcgcgttaat acgctgaaaa tctccgttgc tgatttcctg caattaaccg
    23581 ctccttatgg ctggacgctt acgccaattc cgtggtgtga agaaggtttc tggattgaac
    23641 gcgacaatga agatgcattg ccattgggta gtaccaccga gcatttaagt ggcctgtttt
    23701 atattcagga agccagttca atgttgcccg tcgccgcctt gtttgctgac ggtaatgcac
    23761 cacagcgggt gatggatgtc gctgctgcgc caggctcaaa aacgacgcaa attgccgcgc
    23821 ggatgaataa cgaaggggca atccttgcca atgagttttc cgccagttgg gtaaaagtgt
    23881 tacatgccaa tatcagccgc tgtggcatca gtaatgttgc gctcacacat tttggtggcc
    23941 gcgtgtttgg tgcggcagtg ccagaaatgt ttgatgccat tttgctggac gctccctgct
    24001 ccggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa aaactggtca cccgaaagca
    24061 atcaggaaat cgcagctacc caacgggaac tgattgacag cgcctttcat gcattacgtc
    24121 ctggcggtac gctggtttac tcgacctgta ccttaaaccg ggaagaaaac gaagccgttt
    24181 gccggtggct gaaagagact taccccgacg aagtagagtt tttaccactt ggcgatctct
    24241 tccctggtgc aaacaaggcg ctgaccgaag aaggtttttt gcatgttttc ccacaaattt
    24301 acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac tcaggcgatc cccgccttac
    24361 ccgccccaaa atacaaagtg ggcaatttcc cgttcagccc ggtgaaagat cgcgaagccg
    24421 gacaaattcg tcaggcggct gcaagtgttg gcttaaactg ggatggaaac ctgcgactct
    24481 ggcaacgcga caaagaactg tggttgttcc cggtaggcat tgaagccctg atcggtaaag
    24541 tccgattttc tcgcttaggg attaaacttg ccgaaacgca taacaaaggt tatcgctggc
    24601 agcatgaagc ggttattgcc catgcctccc ccgacaatgt aaacgctttt gaactgacac
    24661 cgcaggaagc ggaggagtgg tatcgcgggc gcgatgttta cccgcaagcc gcgccagtgg
    24721 cggatgacgt gttggttact ttccagcatc agccgattgg tttagccaaa cggattggtt
    24781 cgcgactgaa aaacagctac ccgcgtgaac tggtgcggga cgggaaactt tttaccagta
    24841 acgcatgaca gcgcacaaaa aaagcgcact ttttgactgg cacattcgcc ggcctcaact
    24901 aggctgaaaa attgtgcgat cggactggtc gtactacaat cagcagctaa atggagagca
    24961 cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat cgacgacggc gaattacacg
    25021 gtgaatcgcc gggtgatcga acgttaacca ttccttgtaa atctgacccc gatttatgta
    25081 tgcaactgga tgcctgggat gctgaaacca gtatccctgc cctgctaaat ggcgaacact
    25141 ctgtccttta ccgtacccgt tacgatcaac aatctgatgc ctggattatg cgtcttgcct
    25201 gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc tggttattcc cccgttgtaa
    25261 aatctctcct aaacttaacg gtacggcacc acacttcggg gatgaaatgt tcgcgctggt
    25321 actttttgtt tgctacctag atggcggttg tgaagatatt gttgtggatg tctacaacac
    25381 ggaacagcag tgtctttatt ctatgagcga tcaacggatc cgccatggcg gttgttttcc
    25441 gattgaggat tttatagatg gtttctggcg accagcacag gagtatggtg atttttaatt
    25501 attgcagttg cacaagagtc agttagcccc caaagacagc accggtatca atataatgca
    25561 ggttgccaat atccacgcga tgtcgcaacg gtgtatgacc aaaccagaaa tgatcagcac
    25621 ctgtaattcc ctgccctttt gggcgttcac ctaatcgcga gcggctccac aagacctgat
    25681 gcaaatcaac gtccttttgc cattcat
//
LOCUS       DAECOD010000054        24360 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.054, whole genome shotgun sequence.
ACCESSION   DAECOD010000054 DAECOD010000000
VERSION     DAECOD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.054"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            616..2544
                     /gene="thrS"
                     /locus_tag="KKO15_002264"
     CDS             616..2544
                     /gene="thrS"
                     /locus_tag="KKO15_002264"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBD6361035.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2548..3090
                     /gene="infC"
                     /locus_tag="KKO15_002265"
     CDS             2548..3090
                     /gene="infC"
                     /locus_tag="KKO15_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBD6361036.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3187..3384
                     /gene="rpmI"
                     /locus_tag="KKO15_002266"
     CDS             3187..3384
                     /gene="rpmI"
                     /locus_tag="KKO15_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBD6361037.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKTTKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3437..3793
                     /gene="rplT"
                     /locus_tag="KKO15_002267"
     CDS             3437..3793
                     /gene="rplT"
                     /locus_tag="KKO15_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBD6361038.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3910..4034
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3916..3960
                     /gene="pheM"
                     /locus_tag="KKO15_002268"
     CDS             3916..3960
                     /gene="pheM"
                     /locus_tag="KKO15_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBD6361039.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4243..5226
                     /gene="pheS"
                     /locus_tag="KKO15_002269"
     CDS             4243..5226
                     /gene="pheS"
                     /locus_tag="KKO15_002269"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBD6361040.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5241..7628
                     /gene="pheT"
                     /locus_tag="KKO15_002270"
     CDS             5241..7628
                     /gene="pheT"
                     /locus_tag="KKO15_002270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBD6361041.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7633..7932
                     /gene="ihfA"
                     /locus_tag="KKO15_002271"
     CDS             7633..7932
                     /gene="ihfA"
                     /locus_tag="KKO15_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBD6361042.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8033..9013
                     /gene="btuC"
                     /locus_tag="KKO15_002272"
     CDS             8033..9013
                     /gene="btuC"
                     /locus_tag="KKO15_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBD6361043.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9076..9627
                     /gene="btuE"
                     /locus_tag="KKO15_002273"
     CDS             9076..9627
                     /gene="btuE"
                     /locus_tag="KKO15_002273"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBD6361044.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVALGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9627..10376
                     /gene="btuD"
                     /locus_tag="KKO15_002274"
     CDS             9627..10376
                     /gene="btuD"
                     /locus_tag="KKO15_002274"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBD6361045.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10454..10918
                     /locus_tag="KKO15_002275"
     CDS             10454..10918
                     /locus_tag="KKO15_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6361046.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11165..11878
                     /gene="ydiV"
                     /locus_tag="KKO15_002276"
     CDS             11165..11878
                     /gene="ydiV"
                     /locus_tag="KKO15_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBD6361047.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11942..13378
                     /locus_tag="KKO15_002277"
     CDS             11942..13378
                     /locus_tag="KKO15_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBD6361048.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYLRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13382..13573)
                     /gene="hemP"
                     /locus_tag="KKO15_002278"
     CDS             complement(13382..13573)
                     /gene="hemP"
                     /locus_tag="KKO15_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBD6361049.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13705..14751)
                     /gene="aroH"
                     /locus_tag="KKO15_002279"
     CDS             complement(13705..14751)
                     /gene="aroH"
                     /locus_tag="KKO15_002279"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBD6361050.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14908..15741)
                     /gene="ppsR"
                     /locus_tag="KKO15_002280"
     CDS             complement(14908..15741)
                     /gene="ppsR"
                     /locus_tag="KKO15_002280"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBD6361051.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16073..18451
                     /gene="ppsA"
                     /locus_tag="KKO15_002281"
     CDS             16073..18451
                     /gene="ppsA"
                     /locus_tag="KKO15_002281"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBD6361052.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18508..20154)
                     /gene="fadK"
                     /locus_tag="KKO15_002282"
     CDS             complement(18508..20154)
                     /gene="fadK"
                     /locus_tag="KKO15_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBD6361053.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20211..20504)
                     /locus_tag="KKO15_002283"
     CDS             complement(20211..20504)
                     /locus_tag="KKO15_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD6361054.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20501..21790)
                     /locus_tag="KKO15_002284"
     CDS             complement(20501..21790)
                     /locus_tag="KKO15_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6361055.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21846..22784)
                     /locus_tag="KKO15_002285"
                     /pseudo
     CDS             complement(21846..22784)
                     /locus_tag="KKO15_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22804..23568)
                     /locus_tag="KKO15_002286"
     CDS             complement(22804..23568)
                     /locus_tag="KKO15_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBD6361056.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23884..>24360
                     /locus_tag="KKO15_002287"
     CDS             23884..>24360
                     /locus_tag="KKO15_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBD6361057.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAI"
BASE COUNT         6015 a         5915 c         6409 g         6021 t
ORIGIN      
        1 ggctctgatg aaggtgttgg tatgtcgcta tcgatgtgca atctatgagg agacttataa
       61 tgagttctga taatgatatc gttttgactc ataatctatt cgccttatat atatctggag
      121 gttttgagga tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa aaattgttat
      181 aagaattgtg tcacttttat cattgtgata tgaccacttc gtcattaatc tcatgagcgt
      241 ttaaaatact accgccaaat cacgcaacga actttgctgg attcagttta tgctgctgta
      301 aatccgctcg cagtaaacct ttcagacgca ccgtgatgtt atcagtggtt ctttaagcgt
      361 tttgctggtg tactcactac aaacgaattg cgaatcgaat caatgtgaaa cggaaaggta
      421 caatctccct ttttttagtg ttaacatcgt tcaaccgggt ggaaggtgag aggttaatgt
      481 ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga tggtttcgca
      541 atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg tcaccactgc
      601 aaataaggat ataaaatgcc tgttataact cttcctgatg gcagccaacg ccattacgac
      661 cacgctgtaa gccccatgga tgttgcgctg gacattggtc caggtctggc gaaagcctgt
      721 atcgcagggc gcgttaatgg cgaactggtt gatgcttgcg atctgattga aaacgacgca
      781 caactgtcga tcattaccgc caaagacgaa gaaggtctgg agatcattcg tcactcctgt
      841 gcgcacctgt tagggcatgc gattaaacaa ctttggccgc ataccaaaat ggcaatcggc
      901 ccggttattg acaacggttt ttattacgac gttgatcttg accgcacgtt aacccaggaa
      961 gatgtcgaag cactcgagaa gcggatgcat gagcttgctg agaaaaacta cgacgtcatt
     1021 aagaagaaag tcagctggca cgaagcgcgt gaaactttcg ccaaccgtgg ggagagctac
     1081 aaagtttcca ttcttgacga aaacatcgcc catgatgaca agccaggtct gtacttccat
     1141 gaagaatatg tcgatatgtg ccgcggtccg cacgtaccga acatgcgttt ctgccatcat
     1201 ttcaaactaa tgaaaacggc aggggcttac tggcgtggcg acagcaacaa caaaatgttg
     1261 caacgtattt acggtacggc gtgggcagac aaaaaagcac ttaacgctta cctgcagcgc
     1321 ctggaagaag ccgcgaaacg cgaccaccgt aaaatcggta aacagctcga cctgtaccat
     1381 atgcaggaag aagcgccggg tatggtattc tggcacaacg acggctggac catcttccgt
     1441 gaactggaag tgtttgttcg ttctaaactg aaagagtacc agtatcagga agttaaaggt
     1501 ccgttcatga tggaccgtgt cctgtgggaa aaaaccggtc actgggacaa ctacaaagat
     1561 gcaatgttca ccacatcttc tgagaaccgt gaatactgca ttaagccgat gaactgcccg
     1621 ggtcacgtac aaattttcaa ccaggggctg aagtcttatc gcgatctgcc gctgcgtatg
     1681 gccgagtttg gtagctgcca ccgtaacgag ccgtcaggtt cgctgcatgg cctgatgcgc
     1741 gtgcgtggat ttacccagga tgacgcgcat atcttctgta ctgaagaaca aattcgcgat
     1801 gaagttaacg gatgtatccg tttagtctat gatatgtaca gcacttttgg cttcgagaag
     1861 atcgtcgtca aactctccac tcgccctgaa aaacgtattg gcagcgacga aatgtgggat
     1921 cgtgctgagg cggacctggc ggttgcgctg gaagaaaaca acatcccgtt tgaatatcaa
     1981 ctgggtgaag gcgctttcta cggtccgaaa attgaattta ccctgtatga ctgcctcgat
     2041 cgtgcatggc agtgcggtac agtacagctg gacttctcct tgccgtctcg tctgagcgcc
     2101 tcctatgtgg gcgaagacaa cgagcgtaag gtaccggtaa tgattcaccg cgcaattctg
     2161 gggtcgatgg agcgtttcat cggtatcctg accgaagaat tcgctggttt cttcccgacc
     2221 tggcttgcgc cagttcaggt tgttatcatg aatattaccg attcacagtc tgaatacgtt
     2281 aacgaattga cgcaaaaact atcaaatgcg ggcattcgtg ttaaagcaga cttgagaaat
     2341 gagaagattg gctttaaaat ccgcgagcac actttgcgtc gcgtcccata tatgctggtc
     2401 tgtggtgata aagaggtgga atcaggcaaa gttgccgttc gcacccgccg tggtaaagac
     2461 ctgggaagca tggacgtaaa tgaagtgatc gagaagctgc aacaagagat tcgcagccgc
     2521 agtcttaaac aattggagga ataaggtatt aaaggcggaa aacgagttca aacggcgcgc
     2581 cctaaccgta tcaatggcga aattcgcgcc caggaagttc gcttaacagg tctggaaggc
     2641 gagcagcttg gtattgtgag tctgagagaa gctctggaga aagcagaaga agccggagta
     2701 gacttagtcg agatcagccc taacgccgag ccgccggttt gtcgtataat ggattacggc
     2761 aaattcctct atgaaaagag caagtcttct aaggaacaga agaaaaagca aaaagttatc
     2821 caggttaagg aaattaaatt ccgtcctagt acagatgaag gcgactatca ggtaaaactc
     2881 cgcagcctga ttcgctttct cgaagagggt gataaagcca aaatcacgct gcgtttccgc
     2941 ggtcgtgaga tggcgcacca gcaaatcggt atggaagtgc ttaatcgcgt gaaagacgat
     3001 ttgcaagaac tggcagtagt cgaatccttc ccaacgaaga tcgaaggccg ccagatgatc
     3061 atggtgctcg ctcctaagaa gaaacagtaa ggccttcaag tagcactatc tgtggagcct
     3121 ttgggcttca caggcattgt tcgcctttgt ttcgtttatt aacaatgcga agtggaagtt
     3181 attaaaatgc caaaaattaa gaccgtacgc ggtgctgcta agcgcttcaa aaaaaccggt
     3241 aaaggtggtt ttaagcacaa gcacgctaac ctgcgtcaca ttctgactaa aaaaacgacc
     3301 aaacgtaaac gtcacctgcg tccgaaagcc atggtttcca aaggcgatct gggcctggta
     3361 atcgcgtgcc tgccgtacgc ataagccgtt aacgttttta actttttaat tagaatatag
     3421 atacaggaga gcacatatgg ctcgcgtaaa acgtggtgtt attgcacgcg cacgtcacaa
     3481 gaaaattttg aaacaagcta aaggctacta cggtgcgcgt tctcgcgtat accgcgttgc
     3541 cttccaggct gttatcaaag ctggtcagta tgcttaccgt gaccgtcgtc aacgtaagcg
     3601 tcagttccgt caactgtgga ttgcgcgtat caacgcagca gcacgtcaga acggtatttc
     3661 ttacagcaaa ttcatcaatg gcctgaaaaa agcctctgtt gaaatcgacc gtaagatcct
     3721 ggctgatatc gcagtattcg acaaagtagc gttcaccact ctggttgaaa aagcgaaagc
     3781 agctctggca taagccagtt gaaagaggga gctagtctcc ctcttttcgt ttcaacgcca
     3841 tcaaaacatt gacttttatc gccgtagcct tttcaataaa ggtcttttga agagtaccaa
     3901 aaggtaacgc aagcaatgaa tgctgctatt ttccgcttct ttttttactt tagcacctga
     3961 atccaggagg ctagcgcgtg agaagagaaa cggaaaacag cgcctgaaag cctcccagtg
     4021 gaggcttttt ttgtatgcgc gttttgaaaa ataaaaagcc agtctatcag gcgtaattgt
     4081 cgcagccagt ctggacacgg ccagtgcgga gaaaccggag cgtacttaag tacgtgagga
     4141 tttcgagcac tgcccgggac caaaatggca agtaaaatag cctgatggga taggctctaa
     4201 gtccaacgaa ccagtgtcac cactgacaca atgaggaaaa ccatgtcaca tctcgcagaa
     4261 ctggttgcca gtgcgaaggc ggccattagc caggcgtcag atgttgccgc gttagataat
     4321 gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc ttcagatgac gaccctgcgt
     4381 gagctgccgc cagaagagcg tccggcagct ggtgcggtta tcaacgaagc gaaagagcag
     4441 gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa gcgctgcact gaatgcgcgt
     4501 ctggcggcgg aaacgattga tgtctctctg ccaggtcgtc gcattgaaaa cggcggtctg
     4561 catccggtta cccgtaccat cgaccgtatc gaaagtttct tcggtgagct tggctttacc
     4621 gtggcaaccg ggccggaaat cgaagacgat tatcataact tcgatgctct gaacattcct
     4681 ggtcaccacc cggcgcgcgc tgaccacgac actttctggt ttgacgctac ccgcctgctg
     4741 cgtacccaga cctctggcgt acagatccgc accatgaaag cccagcagcc accgattcgt
     4801 atcatcgcgc ctggccgtgt ttatcgtaac gactacgacc agactcacac gccgatgttc
     4861 catcagatgg aaggtctgat tgttgatacc aacatcagct ttaccaacct gaaaggcacg
     4921 ctgcacgact tcctgcgtaa cttctttgag gaagatttgc agattcgctt ccgtccttcc
     4981 tacttcccgt ttaccgagcc ttctgcagaa gttgacgtca tgggtaaaaa cggtaaatgg
     5041 ctggaagtgc tgggctgcgg gatggtgcat ccgaacgtgc tgcgtaacgt tggcatcgac
     5101 ccggaagttt actctggttt cgccttcggg atggggatgg agcgtctgac tatgttgcgt
     5161 tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct caaacagttt
     5221 aaataaggca ggaatagatt atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc
     5281 cggcgattga tagcgatacg ctggcgaatc aaatcactat ggcgggcctg gaagttgacg
     5341 gtgtagaacc ggttgccggt agcttccacg gcgtggtcgt tggtgaagtg gttgagtgtg
     5401 cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc ggcgatcgcc
     5461 tgctggacat cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgtg gcggtagcta
     5521 ccattggtgc tgttctgccg ggtgatttca aaattaaagc ggcgaaactg cgtggcgaac
     5581 cgtctgaagg gatgctgtgc tccttttctg agctgggaat ttctgacgat cacagtggca
     5641 ttatcgaact gcctgcggat gcgccgattg gcaccgacat ccgcgaatac ctgaaactcg
     5701 atgataacac catcgaaatc agcgtgacac caaaccgtgc cgactgctta ggtatcattg
     5761 gtgttgcgcg tgacgttgcc gtgctgaatc agctgccgct ggttgaaccg gaaatcgttc
     5821 cggttggtgc gaccatcgac gacacgttgc cgattacagt cgaagcgccg gaagcctgcc
     5881 cgcgctatct tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga
     5941 tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt gacgtcacca
     6001 actatgtact gctcgaactg ggccagccga tgcacgcttt cgataaagat cgcattgaag
     6061 gcggcattgt ggtgcggatg gcaaaagagg gcgaaacgct ggtgctgctt gatggcactg
     6121 aagcgaagct gaatgctgac actctggtca tcgccgacca caacaaggcg ctggcgatgg
     6181 gcggcatctt tggtggcgaa cactctggcg tgaatgacga aacgcaaaac gtcttgctgg
     6241 aatgcgcttt cttcagcccg ctgtctatca ccggtcgtgc tcgtcgtcat ggcctgcata
     6301 ctgatgcgtc tcaccgttat gagcgtggcg ttgatccggt actgcagcac aaagcgatgg
     6361 aacgtgcgac ccgtctgctg atcgacatct gcggtggtga ggctggcccg gtaattgata
     6421 tcaccaacga agcaacgctg ccgaagcgtg caaccatcac tctacgtcgt agcaaactgg
     6481 atcgcctgat aggccatcat attgctgatg agcaggtaac tgacattctg cgtcgtctcg
     6541 gctgcgaagt gaccgaaggc aaagacgaat ggcaggcagt tgcgccgagc tggcgtttcg
     6601 atatggagat tgaagaagat ctggtcgaag aagtcgcgcg tgtttacggc tacaacaaca
     6661 tcccggatga gccggtacag gcaagcctga ttatgggtac tcaccgtgaa gctgacctgt
     6721 cgctcaagcg cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg atcacctaca
     6781 gcttcgttga tccgaaagtg cagcagatga tccatccagg cgttgaagcc ttactgctgg
     6841 caagcccgat ctctgttgaa atgtcagcaa tgcgtctttc tctgtggacc ggcctgctgg
     6901 caaccgtggt gtacaaccag aaccgtcagc agaaccgtgt gcgcattttc gaaagcggtc
     6961 tgcgtttcgt accagatact caggcaccgt tgggcattcg tcaggatctg atgttagccg
     7021 gtgtgatttg cggtaaccgt tacgaagagc actggaacct ggcaaaagag accgttgatt
     7081 tctatgattt gaaaggcgac cttgaatccg ttctcgacct gaccggtaaa ctgaatgagg
     7141 ttgagttccg tgcagaagcg aatccggcac tgcatccggg gcaatccgca gcgatttatc
     7201 tgaaaggtga acgtattggt tttgttggag ttgttcatcc tgaactggaa cgtaaactgg
     7261 atcttaacgg tcgcactctg gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg
     7321 tgcctcaggc gcgcgagatt tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg
     7381 tcgcagaaaa cgttcccgca gcggatattt tatccgaatg taagaaagtt ggcgtaaatc
     7441 aggtagttgg cgtaaactta tttgacgtgt accgcggtaa gggtgttgcg gaggggtata
     7501 agagcctcgc cataagcctg atcctgcaag ataccagccg tacactcgaa gaagaggaga
     7561 ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag gcatcattga
     7621 gggattgaac ctatggcgct tacaaaagct gaaatgtcag aatatctgtt tgataagctt
     7681 gggcttagca agcgggatgc caaagaactg gttgaactgt ttttcgaaga gatccgtcgc
     7741 gctctggaaa acggcgaaca ggtgaaactc tctggttttg gtaacttcga tctgcgtgat
     7801 aagaatcaac gcccgggacg taacccgaaa acgggcgagg atattcccat tacagcacgg
     7861 cgcgtggtga ccttcagacc cgggcagaag ttaaaaagcc gggtcgaaaa cgcttcgccc
     7921 aaagacgagt aatctgatct aactaaaaag gccgctatgc ggcctttttt cttttcactg
     7981 tcgaagagtc accgtaaaat caacgccatg acacttcagc agaacggata ccatgctgac
     8041 acttgcccgc caacaacagc gacaaaatat tcgctggtta ttatgcctgt cagttttgat
     8101 gctgctggcg cttctcttaa gcctttgcgc cggtgaacaa tggatttcgc caggtgactg
     8161 gtttactcct cgtggcgaac tgttcgtctg gcaaattcgc ctgccacgta cgctggctgt
     8221 attgctggtt ggtgcggcgc tggctatatc cggcgctgta atgcaggcgt tgtttgaaaa
     8281 tcctctggca gaacctggac tacttggcgt ctctaacggc gcaggcgtgg ggcttatcgc
     8341 cgcggtattg cttgggcaag ggcaactccc caactgggcg ctagggctgt gtgcgattgc
     8401 tggcgcgctt atcatcactt taatactctt acgtttcgcc cgtcgtcatc tttcgaccag
     8461 tcggttattg ctggctggcg ttgcattagg gattatctgc agcgcactaa tgacgtgggc
     8521 tatctacttt tccacctctg ttgatttacg tcagctgatg tactggatga tgggcggttt
     8581 tggcggcgta gactggcggc aaagctggct gatgctggca ttgatccccg tgttgttgtg
     8641 gatctgttgt cagtccaggc cgatgaatat gttagcactt ggcgagatct cggcgcggca
     8701 actgggttta cccatgtggt tctggcgcaa tgtgctggtg gcagcgaccg gctggatggt
     8761 tggcgtcagt gtggcgctgg cgggtgctat cggctttatt ggtctggtga tcccacatat
     8821 tcttcggttg tgtggtttaa ccgatcatcg cgtattactt cccggctgcg cgctggcagg
     8881 ggcgagcgca ttgctgctgg ccgatattgt agcgcgcctg gcattagctg ccgcagagct
     8941 gcctattggc gtggtcaccg caacgttggg tgcgccggtg tttatctggt tattgttaaa
     9001 agcaggacgt tagccgcaaa aagacggtct atgattaaaa gctaagattt ttactgacca
     9061 cacctaggag aaacgatgca agattccatt ctgacgaccg tagtgaaaga tatcgacggt
     9121 gaagtgacca cgctggagaa gtacgccggt aatgtgctgt taattgtcaa tgtcgcctca
     9181 aagtgtggct taacgccgca atatgagcag ttggagaata ttcaaaaagc ctgggccgat
     9241 cgaggttttg tggcgctggg attcccgtgc aaccagtttc tggaacaaga accgggcagc
     9301 gatgaagaga ttaaaactta ctgtaccacc acatgggggg tgacgttccc gatgttcagt
     9361 aagattgaag ttaatggcga aggacgccat ccgctgtatc aaaaattgat tgccgcagcg
     9421 ccgaccgcag tcgcgccgga agagagcgga ttctatgccc gtatggtcag caaaggccgt
     9481 gcaccgctgt acccggatga tattttatgg aattttgaaa aattcctggt tggcagggac
     9541 ggaaaagtca tccagcgttt ttccccggat atgacgccgg aagatcccat tgtgatggaa
     9601 agcattaaac tggcgttggc aaaataatgt ctattgtgat gcagttacag gatgttgcgg
     9661 aatctacccg cctggggccg ctttcaggcg aggttcgggc tggggaaatc ctgcacctgg
     9721 tggggccgaa tggcgcgggt aagagtacct tactggcgca aatggccgga atgaccagcg
     9781 gtaagggaag cattcagttc gcggggcaac cactggaagc atggtccgca acaaaactcg
     9841 cgctgcatcg cgcctatctt tcacaacagc agacgccgcc gtttgcaatg ccggtctggc
     9901 actacctgac actgcatcag cacgataaaa cgcgtaccga actactgaat gatgtcgcag
     9961 gggcgctggc tcttgatgac aaactcggtc gtagcaccaa tcaactttcc ggcggtgaat
    10021 ggcaacgcgt acgtcttgct gcggtggtgt tgcaaatcac accacaagcc aatcccgcag
    10081 gccaattgct gcttcttgat gagccgatga acagtcttga tgttgcgcaa caaagtgcgt
    10141 tagacaaaat tctgagcgcg ctgtgtcagc aaggactggc gattgtgatg agcagtcacg
    10201 atctcaacca cacgttgcgt catgcgcatc gggcgtggtt gctaaaaggt ggaaaaatgc
    10261 tggccagtgg acgcagagaa gaggtgctca cgccgccaaa tctggcgcag gcctatggga
    10321 tgaattttcg ccgtctggat atcgaaggtc acagaatgct gatttcgacc atctgatttc
    10381 attagtatcc cgaaaatcgg gtaattacga cgctaaatta atgcaagaat aaaaaacaga
    10441 ggaaccgtac ggaatgcgtt tctgccttat tttgatcaca gcactgtttc tggccgggtg
    10501 tagccaccat aaagcaccgc cgccaaatgc cagactttct gattcgatta ccgttattgc
    10561 cggtttgaac gaccagctac aaagctggca tggcacgccg tatcgttatg gtggcatgac
    10621 gcggcgcggt gtggactgtt cgggatttgt ggttgtgacg atgcgcgatc gtttcgattt
    10681 gcatctgccc cgtgaaacca aacaacaagc ctctattggc acgcaaattg ataaagacga
    10741 gttgctgcct ggtgacctgg tctttttcaa aacgggttcc ggacaaaatg gtttgcatgt
    10801 aggtatttac gataccaaca accaatttat ccacgcctct accagcaagg gagtgatgcg
    10861 ttcctcactt gataatgtct attggcagaa aaatttctgg caggcgagac gaatctagta
    10921 tgtcgaaaaa ttgacggcga tgatacatcg ccgtcaattt actcctgaat acagtagtat
    10981 tttgcagaca aaaattccgt aaatggatta ttcgtttata atcaggataa tctaaaaaaa
    11041 ggccccctga aaaagtggtt aacatggaac aaatgaaagt tactccttct gtaattgtta
    11101 tgtcttatgg cctcatccct tttagccgga tactgaaaaa catccttcga gagggacggt
    11161 taccatgaag atttttttgg agaatcttta tcattctgat tgctatttcc tgccgataag
    11221 agataaccag caactccttg tcggcgttga gttaattacc catttctcca gtgaagatgg
    11281 cacggttcgt atcccgacga gtcgggttat agcgcaactt accgaggagc aacattggca
    11341 attattttca gagcagttgg aattactaaa atcatgccaa cactttttta ttcaacataa
    11401 actctttgcc tggcttaatt taacgccaca agtggctaca ttattattag acagggataa
    11461 ttacgcaggc gaactattaa aatatccgtt tattgagctg ttgattaatg agaattaccc
    11521 gcatctcaat gaaggaaaag ataatcgcga tcttttgtcc ttatcgcaaa tgtatcccct
    11581 tgtgttaggc aatttgggag caggtaatag cacaatgaaa gcggtttttg atggtctttt
    11641 cacccgcgta atgctggaga aaggttttat tcagcaacaa atcacgcatc gctccttcga
    11701 accgtttatt cgtgcgatcc aggcgcaaat ttccccttgt tgcaattgca tcatcgcggg
    11761 gggcattgat acgccagaaa tattggcaca gattaccccc ttcgatttcc atgctctcca
    11821 gggatgcctg tggcctgccg ttcctataaa tcagataacg acgttggttc agcgataacc
    11881 ctcctgtttg ctggtgttta agacgagagt aaccgtctac actatcaaac agataggatc
    11941 tatgaccctg tcttttatta cccgctggcg cgatgaattg ccagaaacct atacagcact
    12001 ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac tcgctaacac
    12061 gctgagtatt ccatcgtcgc tgtttaaaaa tgccgcaggt gtctggggcg gcgaaaccct
    12121 actgcctggt atgtcaccac tggcacaggt ttacagtggt catcagttcg tcgtctgggc
    12181 gggccaactg ggtgatgggc gcggcatttt acttggcgaa caactgcttg ctgatggcac
    12241 tacaatggac tggcatctga aaggtgctgg cctgacgcct tatttgcgaa tgggtgatgg
    12301 acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga tgcattatct
    12361 gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag tgtatcggga
    12421 aacggtggag ccaggcgcga tgctgatgcg tgtggcacca agtcatctgc gctttggtca
    12481 tttcgaacat ttttactatc gccgcgagcc ggaaaaggtt cgtcagttgg ctgactttgc
    12541 cattcgtcat tactggtcac atcttgaaga cgatgaggac aaataccgtc tctggtttaa
    12601 cgatgttgtc gcacgtaccg cgtcgttaat tgcccaatgg cagacggtcg gctttgcaca
    12661 tggggtgatg aataccgaca acatgtcgct gctgggactg acgcttgatt acgggccgtt
    12721 tggttttctt gatgattacg aacccggttt tatttgtaat cactcggatc atcaagggcg
    12781 ttatagcttt gataatcaac ctgctgtcgc gttgtggaat ttacagcgtc tggcgcagac
    12841 attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct atcagcaggt
    12901 tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatga cggagcaaaa
    12961 agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc gcagcgatta
    13021 tacccgcaca ttccgcatgc tgagtctgac cgagcagcac agcgcggcgt caccgctacg
    13081 tgaggagttt attgatcgtg cggcatttga tgactggttt gcccgttatc gggggcgttt
    13141 gcaacaagac gaggttagcg atagtgagcg tcagcaactg atgcaaagcg ttaacccagc
    13201 tctggtattg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat
    13261 gatggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca gagatgatga
    13321 ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct cgagttaaag
    13381 gctacttagt taacagcagc ttgccagcct gagttttacg gagcagatat tcttgcccgt
    13441 catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggta attcgccgga
    13501 tgggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt tttctgctat
    13561 ccgtataacg cataaaaatt aataataatt gatataacaa tgattcctat tatcaatatt
    13621 atggcaggcc agagcaagca gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc
    13681 atcccatcag gaatgggcac gcattcagaa gcgggtatct accgcaaagg cgagtttttc
    13741 gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt gaccgtaagt
    13801 gagcggctga ccgccgacga ttttttgcgt tccttcgcgc aggaaacttt ccgccataat
    13861 tccagcaatc tccgtagagc cattgcggat ttgctgacaa atatcctcac aaacttctaa
    13921 ctggcgacgg tgctgcttct ggcagttacc gtggctgaaa tccaccacca gatgttcagg
    13981 taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat gataattcgg
    14041 ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct gatagatggt
    14101 catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg cgcggatagc
    14161 atccacggca atccgcgtgt tgccatcggt accattttta aaacctaccg gacaggagag
    14221 tgctgaagcc atttcgcggt ggatctgact ttcggtagta cgagcgccaa tcgcgcccca
    14281 actgattaaa tcagcaataa actgaccggt taccatatcg aggaactcag tcgcggtagg
    14341 gacacccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac cgtgatttac
    14401 ccgatagctg ccgtttaaat ccggatcgga gattagccct ttccagccga caacagttcg
    14461 tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact ggttgcgcag
    14521 cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg agcaggggcc
    14581 aataatgacc aacagtcgct tatcttcacc attcagtatt ttttcaattc tgcggcggga
    14641 gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga gttcggcggg
    14701 cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca ttacaagtct
    14761 cagaaatttg ttgcttctac ggcaggacta ccggaaagtg atgcgcatca cgataaacca
    14821 atcgcagctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta gcatgataac
    14881 aaaaaaacaa accaatgcac tagttctcta gtacattcgg cgactaaggc ccatgatatc
    14941 gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc acgggatctg
    15001 atttttacgg tacaaggctt ccacttccgc gacttccatc ctgcactgac gaagcgaggc
    15061 atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt
    15121 caggccgaac aatttatgct gaagcggttt gagcgacgcg ggtagcacca gattatccat
    15181 atcgtcggca ataaaggggt agtttgccgc gcggatacca aattgcattg ccagatacag
    15241 actggtgggg gttttaccac agcgagaaac accgagcagg atcacctgag cctgatccag
    15301 attgcgcaac gaaatgccgt catcgtgggc gagggtgtaa tcaatcgccg caatgcgcgc
    15361 atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa tcggcgttgg
    15421 atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa
    15481 gccttcactt tgcaagatga tggcgcgaat ctccggcaac acgatggagt agaagaccag
    15541 cgggcgcacg cctgtctggt gataaattgc gtcaatctga tccttcactg cccgtgcacg
    15601 gctctcattt tcgacaaacg gcagcgtgat gctgctgata gtgacgggaa attgtgacat
    15661 tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg
    15721 gcgatcaaca gcattatcca ttttgttctt cccgtgaagc agacataatt ctatgaaagc
    15781 ataaattaaa agcgcacaga agcgtaggac gtaatgtctg gtttataaaa tgaaccttca
    15841 ttttattttt tataaaaaca gcatttcatt tttatggttt cgtttatacc gatggtttat
    15901 gtggaaattg tcgaatagag caggtttgcg caacgctggg atcagtctta aaaagtaaaa
    15961 aaatatattt gcttgaacga ttcaccgttt ttttcatccg gttaaatatg caaagataaa
    16021 tgcgcagaaa tgtgtttctc aaaccgttca tttatcacaa aaggattgtt cgatgtccaa
    16081 caatggctcg tcaccgctgg tgctttggta taaccaactc ggcatgaatg atgtagacag
    16141 ggttgggggc aaaaatgcct ccctgggtga aatgattact aatctttccg gaatgggtgt
    16201 ttccgttccg aatggtttcg ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag
    16261 cggcgtaaac cagcgcattt atgaactgct ggataaaacg gatattgacg atgttactca
    16321 gcttgcgaaa gcgggcgcgc aaatccgcca gtggattatc gacactccct tccagcctga
    16381 gctggaaaac gccatacgcg aagcctatgc acagctttcc gccgatgacg aaaacgcctc
    16441 ttttgcggta cgctcctccg ccaccgcaga agacatgccg gacgcttcct ttgccggtca
    16501 gcaggaaacc ttcctcaacg ttcagggttt tgacgccgtt ctcgtggcag tgaaacatgt
    16561 atttgcttct ctgtttaacg atcgcgccat ctcttatcgt gtgcaccagg gttacgatca
    16621 ccgtggcgtg gcgctctccg ccggtgttca gcggatggtg cgctccgacc tcgcatcttc
    16681 tggcgtgatg ttctccattg ataccgaatc cggctttgac caggtggtgt ttatcacttc
    16741 cgcatggggc cttggggaaa tggtcgtgca gggtgcggtt aacccggatg agttttacgt
    16801 gcataaaccg acactggcgg cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa
    16861 aaaaatccgc atggtttacg cgccgaccca ggagcacggc aagcaggtta aaatcgaaga
    16921 cgtaccgcag gaacagcgtg acatcttctc gctgaccaac gaagaagtgc aggaactggc
    16981 aaaacaggcc gtacaaattg agaaacacta cggtcgccca atggatattg agtgggcgaa
    17041 agatggccac accggcaaac tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg
    17101 cggtcaggtc atggagcgtt atacgctgca ttcacagggt aagattatcg ccgaaggccg
    17161 tgctatcggt catcgcatcg gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat
    17221 gaaccgcatc gaacctggcg acgtgctggt tactgacatg accgacccgg actgggaacc
    17281 gatcatgaag aaagcatctg ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc
    17341 gatcatcgct cgtgaactgg gcattccggc ggtagtgggc tgtggagatg caacagaacg
    17401 gatgaaagac ggtgagaacg tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta
    17461 tgcggagttg ctggaattta gcgtgaaaag ctccagcgta gaaacgatgc cggatctgcc
    17521 gttgaaagtg atgatgaacg tcggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc
    17581 gagcgaaggc gtgggccttg cgcgtctgga atttatcatc aaccgtatga ttggcgtcca
    17641 cccacgcgca ctgcttgagt ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga
    17701 gatgatgaaa ggttttgatt ctccgcgtga attttacgtt ggtcgtctga ctgaagggat
    17761 cgcgacgctg ggtgccgcgt tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa
    17821 atcgaacgaa tatgccaacc tggtcggtgg tgagcgttac gagccagatg aagagaaccc
    17881 gatgctcggc ttccgtggcg cgggccgcta tgtttccgac agcttccgcg actgcttcgc
    17941 actggagtgt gaagcagtga aacgtgtgcg caacgacatg gggctgacca acgttgagat
    18001 catgatcccg ttcgtgcgta ccgtagatca ggcgaaagcg gtggttgaag aactggcgcg
    18061 tcaggggctg aaacgtggcg agaacgggct gaaaatcatc atgatgtgtg aaatcccgtc
    18121 caacgccttg ctggccgagc agttcctcga atatttcgac ggcttctcaa ttggctcaaa
    18181 cgatatgacg cagctggcgc tcggtctgga ccgtgactcc ggcgtggtgt ctgaattgtt
    18241 cgatgagcgc aacgatgcgg tgaaagcact gctgtcgatg gctatccgtg ccgcgaagaa
    18301 acagggcaaa tatgtcggga tttgcggtca gggtccgtcc gaccacgaag actttgccgc
    18361 atggttgatg gaagagggga tcgatagcct gtctctgaac ccggacaccg tggtgcaaac
    18421 ctggttaagc ctggctgaac tgaagaaata aaataaatcc ccggcggcgt tcagtcgccg
    18481 gggttatgtg atccccgaag atgaaactta ttcaatctct tcacagacat cctgcgttaa
    18541 tcgccgtata atatcttttc ttaacaaaaa cttttgtatt ttatctgagg cagtgcgcgg
    18601 tagtttttcg attaccacga tatgttcagg atatttatat tttgcgaccc gtttacggct
    18661 aaaaaaagcc gccatctctt ccagcgataa tgaatgatgc ggcgctttca gcacgacata
    18721 agcgcatgat cgttcaccta aacgttcatc gggcattgca accacacagg catcgtgaat
    18781 tttaggatgc tgcaataaaa tatcttccac ttcacggctg ctaatatttt cgccgccgcg
    18841 gacaataata tcttttttgc gtccggtaat ttttatatag ccagcctcat ccatacggca
    18901 gagatcgccg ctgtaatacc agccttcttc atccagcgca cgggcggtta attcaggttc
    18961 atcaaaatac cccataaaca cattggggcc acgcgatgct tcttcacctt cgcaacctgg
    19021 cggtaaggtc ttgcgtgcgt catcgaccac tttaatctct acacctgcgg cagcgtaacc
    19081 atcggtatgc ataaagcgcg acaaagaatt atcgagattc accaccgcat gcggcgaact
    19141 ttctgtggaa ccataaacac ttaataattt aatgctgcgc tgctggcatt cgcgcgccac
    19201 ttttttgggg attgtggtac cgccgcaaag aaagaagcgc agcgctgaaa ggtctgcggg
    19261 ttgtttctct agtaaattca aaagatcata gacaaacggc gttgcgccta gcatacaggt
    19321 gcaacgctgc tgctcaagca gcgcgagaca cgcatcagga gtgaaaatat ctaacaacac
    19381 gctgcgagcg ccaattaaga atggtgccgt tacgccatgc agaaagcccg ttgcgtgacc
    19441 aagtggcgca ggcatcataa agacatccag ccaggtcaga ttcagccgcg cgcaataagc
    19501 ccgctcactg gcgagaatat tgttatgcgt tagcatcacg ccctttggca gaccctcggt
    19561 tccggaggta aacagcaccg cagctaattc atcgccgtgg gtcgttatcg ccgtggtcag
    19621 tgggatattg tcggcgataa tctgacttaa tgagagggaa gaggtggcgg gagccagttt
    19681 gtccacgccg acaatttgtt gtagttgtgg aagctgattt tgcagcggca ggattaaatc
    19741 taccggacgc gtttgtttaa acaacgtcgg tgcaaagaac atttttgcct gacacttatt
    19801 gagcacccac accagttctg cttcccgcca ggaaggcaac agcggcacgg aaaccgcacc
    19861 gattttcagg caggcaagat agataacggt aaattcacac cagccaggca gttgaaatgc
    19921 gatgcgatcg cctgattcaa tacccttcgc taacatccag tttgccagac agctcgcggc
    19981 gtgatcgagc gcgctatagg tgtacgatgc accatgatta tcgaccacgg caattttgtc
    20041 tggcatcgca cgagcggtct gctgccagta atcggccagt gaagcatcgc cccatagccc
    20101 ttgctgacga tacgccgcac gacgttgttc gttaaacgtt aatgtcactt tcattttgga
    20161 ctctcgaaac agaacatcag gcccgagttg cgggcctgtg ggatgcatga ttagccgtag
    20221 cgaaaatcaa taccgaaggt gcctgcggga tattgccact gttcgagaat agtgttaccg
    20281 cacagcaccc gacaggtgcc gcactccaga caaccggcgg gatcaaaatg aatgtttcct
    20341 acgtcatcct gcttataaag tccggcaggg caggctttca ttaatttacg gaattcattg
    20401 atatcgggat tttccgtcag aatgatatgc gggtggccct catcaacatg gaatttattg
    20461 acgcctaatt tgatgtcgac gttaaccgta gcgttctggc tcatagcgcg gttgctccct
    20521 taatgccatc tttcagcaag ttgatcagcc caattttctt cgcgtgtccc atgatcattt
    20581 tgcgtaccgg ctggtttggt ttgccgtcaa tggtgaacat ctcgttcatg atgtcggcta
    20641 ccattcgtgg gtattggcta aacaggcgcg ggttttccat cagcgccggg atcttgcgaa
    20701 aatgctgcat atcacgcatg acgcagcttt gttccagctc acgtttgtat tgcgccagac
    20761 tgttggcgga gaaatccgcg cgttctttgg cggcgatcac cgttgtggcg gcagcctgag
    20821 ccgatgcaat ggctaaatcc atgccgcgga ccgtaaaacc caaattcagg cagaagcctg
    20881 cggcgtcacc aacgatcatc acgccctcgt taaccagttg cggcaccatt gccagaccgc
    20941 cttccggcac catatgcgcg gaatattcaa gcagtttgcc gccgctaatc agcgggcgaa
    21001 tggcggggtg ttgtttaaaa tcttccagca tttgcggcac gcttttttgc gcatgggcga
    21061 tatcacccaa tccacaaacc agccccaagg atatggaatc cttgttggta tagagaaatc
    21121 ccccgcccat caggccgtca gaaggggaac cggcaaacag ccaggcggca ccttcctcgc
    21181 ccgtaatatt aaagcgatcg ttgatctgtt ctggtgtgag gccaataacc tctttaacac
    21241 caacagcgta atgatgcgga tcggaagcgg gaaccattcc cagcgagcgg ccaagcatcg
    21301 agttaacgcc atcagccaga atcaccacat tcgcttcgag aatatcatcc ccagcctgca
    21361 cgccagtgac cttgtttcct tcacgaacca gcgcgtcgac gcgaacgcct gggataaact
    21421 gcgcgcccgc ctgctcggct tgttccatca accacgggtc cagacgatta cgcaatacgg
    21481 tataagatgc gtgttgagga acatctggtt gctcgcggtg aaaatcgagg gtaacggcgc
    21541 tttcttcggt taagaaggag attttctcgc gtgtgacctt gcgttctacc ggcgctgata
    21601 ctgcaaagcc tggaatgatt gcttcaagtg tgtgggcata aagacgcccg ccggtcatgt
    21661 ttttacatcc ggcactgtcg ccgcgttcta tcaccaggac atccagcccg gctcgtgcca
    21721 tgaccagtgc ggcaacgcta ccagcaacgc ccgcaccgac cacaatggca tcaaatttgt
    21781 catccgacat gcgttatgct cccttcctgc tgcgtttaca gcgggagatg tggttgaaag
    21841 gggaattagc ggctcaactg gctaatcagg gcagggacga ctttgtagat atcgcccacc
    21901 agaccgtagt cggcatagtt gaagattggc gcatttttat ctttattgat ggcgacaatc
    21961 acttttacgc cgttgccgcc aaccatatgt tggatctgcc cggagatccc cagcgtcagg
    22021 tagagatcgg atttcagcaa cacgccggag acaccgatat aacgttcacg ctccatccag
    22081 ttctcgcctt cggcaattgg acgtgaacag ccgacttcag cattcagcac cgccgccagt
    22141 tcgtggacca ttttcaggtc atcctgcgcc gccagaccac gaccgacgcc aaccacacgt
    22201 tttgctttgc tcaggtccac gctgcttgcg gctttcgcac ggcgttcgcg acagagaatt
    22261 tcatgacgcg gagcaacata aggtaccgtt tctgtcgggc actgatgaga ggtatcactg
    22321 gtgcacggtt cctgaacacc gggtgcaagg gtaatgatcg ccagcgggct gttgatcttt
    22381 tcctaagcga acgctaaccc gccatacatc cggtgttcgg cgcaaatgtg accatcgaca
    22441 atatccaccg ccgtggcatc gttcaccagc gccgcattca gttgcacact taaccgtgct
    22501 gccagagctt taccacgttt cgtcgcggcc aacagcaaca tagcggggtg tttatctttc
    22561 agcagggcag caatgctttc ggcgtaattt tcagtgcgtt gcagcgcgtc gttttgctca
    22621 agaacataaa tacattttgg accataaggc ataactgcct gcgcctggtc ggtattttgt
    22681 acaatggcat acacctgttg gccccattgc tgagcgccgc caaacagttc agcataacgt
    22741 tcaggattat cgctaaagac ccagacgctg tttaattgac tcatcgtttc ttctccgtag
    22801 gcttcagttc agggctttct tcagatgttc cgccagctcg gcaatggcct ccggcgaatc
    22861 gttatcgaga atgatgtgct tacgttctgt ttgcggcggt acgcgaatgc cgaccagttc
    22921 agcaagtggc gcgctctggc tccagtcaat atcacttgcc tgccactgat ttaccggttt
    22981 tttacccgcg ccgagaatgg ctttcatcga aggaatgcgt ggcacgttaa tatcggaggt
    23041 gacgcagagc acggctggaa cagagagttc aataacttca acatcatctt caagcgtgcg
    23101 ttcaatcacc agtgtattgc cctgacgctg aatagcactc actgcattaa tcaccggaag
    23161 ttgcagaatt tctccgacca gcaagccaac ctgctgggca taaaggtcgc cggaaccttc
    23221 accaaagatc agtaaatcga agccgatctt ttcaattgct gccgccagcg cttttgcggt
    23281 atcgagaggc agtgcatgtt caagttgcgc atcctgcacc aaatacaggc tgtgcggccc
    23341 gcgggatagc acgtctttgc gcactttcga gttctgcaac aatgagccgc caacggtcag
    23401 cgcggctatc tcatcgtcat ctgttgcgag ctggcttgca gcttcaatgg cattgagatc
    23461 gaactggctg attttggcgt cggcattgtc gaaattcagg gtgtattctg gagtgacaac
    23521 aatgtcctgt tcttcaggca ccagcttaaa gcaggttatt attttcatga tttctcctgt
    23581 gagtcgcata cggaaccatc tccgatggaa ggtaaaaatg gcctgaaaat agtgtgcgat
    23641 agcggtgcgg ggggttcgac ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa
    23701 atgcgattgc taaaacaaaa gtgcaatttg cggcaatata gttattccat gaccgtacaa
    23761 tcttgcattc agtgtaaata atcttgttta gctctcgcta aacgcggttg tataaccgca
    23821 atacacgccg tattttatcc ggcatatatt gtttttgctt gctcatggaa tagggaggtt
    23881 gtcatgtatc aacgctgttt tgataatgcc agtgaaacgc tgtttgtcgc cggtaaaacg
    23941 ccacggcttt cacgttttgc atttagcgat gatccaaaat gggagtctgg acatcacgtt
    24001 catgacaatg aaaccgagct gatttacgtc aagaaagggg ttgcaaggtt taccatcgat
    24061 tcttcgttat atgtcgcaca tgcggatgac attgtggtga tagaacgcgg caggctgcat
    24121 gcggtggcct ctgacgttaa cgatccggca acgacgtgta cctgtgcgct gtacggcttt
    24181 cagtttcagg gggctgagga aaatcagtta ctgcaaccgc attcttgtcc ggtaattgcc
    24241 gcggggcagg gaaaagaagt cataaaaacc ttatttaatg agctaagtgt gattttgccg
    24301 caaagtaaaa atagccaaac atcttcgtta tgggatgcat ttgcctatac attagcaatt
//
LOCUS       DAECOD010000055        24182 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.055, whole genome shotgun sequence.
ACCESSION   DAECOD010000055 DAECOD010000000
VERSION     DAECOD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.055"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="KKO15_002288"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="KKO15_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBD6361058.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="KKO15_002289"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="KKO15_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBD6361059.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="KKO15_002290"
                     /pseudo
     CDS             complement(937..1227)
                     /locus_tag="KKO15_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1661..2371
                     /locus_tag="KKO15_002291"
     CDS             1661..2371
                     /locus_tag="KKO15_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBD6361060.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="KKO15_002292"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="KKO15_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBD6361061.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4149)
                     /locus_tag="KKO15_002293"
     CDS             complement(3499..4149)
                     /locus_tag="KKO15_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBD6361062.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4312..6645
                     /gene="bisC"
                     /locus_tag="KKO15_002294"
     CDS             4312..6645
                     /gene="bisC"
                     /locus_tag="KKO15_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBD6361063.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6614..7054)
                     /locus_tag="KKO15_002295"
     CDS             complement(6614..7054)
                     /locus_tag="KKO15_002295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6361064.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7051..7614)
                     /gene="tag"
                     /locus_tag="KKO15_002296"
     CDS             complement(7051..7614)
                     /gene="tag"
                     /locus_tag="KKO15_002296"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBD6361065.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7772..8470
                     /locus_tag="KKO15_002297"
     CDS             7772..8470
                     /locus_tag="KKO15_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6361066.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8699..9907
                     /locus_tag="KKO15_002298"
     CDS             8699..9907
                     /locus_tag="KKO15_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361067.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10231..11922
                     /gene="eptB"
                     /locus_tag="KKO15_002299"
     CDS             10231..11922
                     /gene="eptB"
                     /locus_tag="KKO15_002299"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBD6361068.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12014..12090
                     /locus_tag="KKO15_002300"
     tRNA            12014..12090
                     /locus_tag="KKO15_002300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12048..12050,aa:Pro,seq:cgg)
     gene            12834..14441
                     /gene="dppA"
                     /locus_tag="KKO15_002301"
     CDS             12834..14441
                     /gene="dppA"
                     /locus_tag="KKO15_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBD6361069.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14749..15768
                     /gene="dppB"
                     /locus_tag="KKO15_002302"
     CDS             14749..15768
                     /gene="dppB"
                     /locus_tag="KKO15_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBD6361070.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15778..16680
                     /gene="dppC"
                     /locus_tag="KKO15_002303"
     CDS             15778..16680
                     /gene="dppC"
                     /locus_tag="KKO15_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBD6361071.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16691..17674
                     /gene="dppD"
                     /locus_tag="KKO15_002304"
     CDS             16691..17674
                     /gene="dppD"
                     /locus_tag="KKO15_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBD6361072.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17671..18675
                     /gene="dppF"
                     /locus_tag="KKO15_002305"
     CDS             17671..18675
                     /gene="dppF"
                     /locus_tag="KKO15_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBD6361073.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18705..19976)
                     /locus_tag="KKO15_002306"
     CDS             complement(18705..19976)
                     /locus_tag="KKO15_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD6361074.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20452..20559
                     /locus_tag="KKO15_002307"
     CDS             20452..20559
                     /locus_tag="KKO15_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6361075.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20935..21042
                     /gene="ldrD"
                     /locus_tag="KKO15_002308"
     CDS             20935..21042
                     /gene="ldrD"
                     /locus_tag="KKO15_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBD6361076.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21418..21525
                     /locus_tag="KKO15_002309"
     CDS             21418..21525
                     /locus_tag="KKO15_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6361077.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21901..22008
                     /locus_tag="KKO15_002310"
     CDS             21901..22008
                     /locus_tag="KKO15_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6361078.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22095..23774)
                     /gene="bcsG"
                     /locus_tag="KKO15_002311"
     CDS             complement(22095..23774)
                     /gene="bcsG"
                     /locus_tag="KKO15_002311"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBD6361079.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23771..23962)
                     /gene="bcsF"
                     /locus_tag="KKO15_002312"
     CDS             complement(23771..23962)
                     /gene="bcsF"
                     /locus_tag="KKO15_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBD6361080.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23959..>24182)
                     /locus_tag="KKO15_002313"
     CDS             complement(23959..>24182)
                     /locus_tag="KKO15_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBD6361081.1"
                     /translation="TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKP
                     VINAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         5844 a         5987 c         6416 g         5935 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 acaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccat atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cactcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcataactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcaaaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcagc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcacacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggagcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaagtaa
     4141 gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg tcacgggttc
     4201 cgtggcggga gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt
     4261 gtaagtaata tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac
     4321 tcatcctcac gatattccgt tctgactgcc gcccactggg ggcccatgct ggttgaaacc
     4381 gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg
     4441 cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg tacgatttcc aatggtgcgc
     4501 aaaggctttc tcgcgtcacc ggaaaacccg caaggcattc gtgggcagga tgaatttgtt
     4561 cgcgtgagtt gggatgaggc gctggatctt attcaccaac aacataaacg cattcgtgag
     4621 gcttatggtc cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg
     4681 cataaggcct cgacattatt acaacgctat atggcgctgg caggcggtta taccgggcat
     4741 ctgggggatt attcgaccgg tgcggcacag gcgatcatgc cgtatgtcgt aggtggcagc
     4801 gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg
     4861 ttgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag
     4921 gggctttctt acttttccgc actgcgtgac agtgggaaaa agctgatctg cattgatcca
     4981 atgcgatcgg aaaccgtcga tttctttggc gataaaatgg agtgggtggc accgcacatg
     5041 ggcaccgatg ttgcgctgat gctggggatc gcccatacgc tggtggaaaa tggttggcac
     5101 gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg
     5161 ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc
     5221 gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa ataccaccat gctgatggca
     5281 ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgat cgtcacgctg
     5341 gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt
     5401 gccaatggtg gtaaccccac gcggcgttct gcggtgctct cttccatgca gggcagcttg
     5461 ccgggtggct gcgatgcggt ggataaaatc cctgttgccc gcattgttga agcactggaa
     5521 aaccctggtg gcgcatatca acacaacggt atgaaccgac atttcccgga tattcgtttt
     5581 atctggtggg cgggcggtgc caactttact catcatcagg ataccaatcg tctgatccgt
     5641 gcctggcaaa aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa
     5701 cacgcggata ttgttctgcc tgcgactacc tcttttgagc gtaatgatct caccatgacc
     5761 ggtgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa
     5821 gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac gttgggagaa gggcggctat
     5881 gcgcggttta cggaaggaaa aagtcagctg caatggctgg aaacgtttta taacgttgcc
     5941 cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc
     6001 aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgctgat
     6061 ttttgccgcg atccgctggc gcatccgtta aaaaccgcca gcggcaagat tgaaatcttc
     6121 tcacagcgta ttgccgatta cggttacccg gattgccctg ggcatccaat gtggctggag
     6181 ccggacgaat ggcagggcaa tgccgaacca gaacagttgc aggtactttc tgcccatccg
     6241 gcgcaccgcc tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtagca
     6301 aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat aaccgaaggg
     6361 gatatggtgc gggtgtggaa ctcccgtggg cagatccttg ccggagcggt cattagcgag
     6421 ggaattaaac ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc
     6481 gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg
     6541 cggctgggga atggctgtgc gggtaatacg acgctggcat ggctggaaaa atacaacggt
     6601 ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt
     6661 gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcta
     6721 tcgccggttg atttttttga taaacctcca gcatcagatg gggatagcgc tgctgcacat
     6781 actgcatcag cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgctg
     6841 ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtcc tcttcccaga
     6901 cccagttttg cgcgttggca agataggcat cccgcaccag cggaatgcag tcacgccagt
     6961 aattcgcttt tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggatcg
     7021 cggggagttc tgaacgttgc gcttcccgaa tcatggttta tttcccagat agcaacagca
     7081 gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt
     7141 gccgacaaac ttaaaaccac gttttttcag tgccttagat agggcgtcgg aggcggacgt
     7201 agatgtggga atttcgctca acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa
     7261 cgaccagaca aagtcgggaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc
     7321 attaccaata attgcctgaa ttttccctcg atggcggata atcccggcgt cctgtaccag
     7381 tctttcgaca tcctcttcct gcattgctgc gaccttcacc ggatcgaact gatgaaagca
     7441 ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc
     7501 ttcaaagcag atcatttcga acagtttttt actgtcagtt tcaggcacgc cccactcatt
     7561 atcatggtag gcaatataaa gcgggccctg actcacccag ccgcaacgtt ccatactttc
     7621 cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa
     7681 gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac aataatattg
     7741 ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg
     7801 gcagctaagc ctgctggcga gcgtggtaat cagtgccttt tttctcaaca cagcttacgc
     7861 ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg
     7921 ggatagtgat catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca
     7981 aagggcgctg ggactggaag tcaatctggc ggaagaaact cctgtggatg tgaccagcag
     8041 tatgagtatg ggctggaatt ttcctttata tgaacaggtt acaaccggcc cggtcgcggc
     8101 attacattac gatggcacaa ccacctcgat gtataacgag tttggcgaca gtactaccac
     8161 gctgaccgat ccgttatggc atgccagcgt gagtagctta ggctggcgtg ttgactcccg
     8221 gcttggcgat ctccgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat
     8281 ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct
     8341 ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgccgcct atgctgcgct
     8401 aactcaggca gaaaatacca ctaataatag cgactatctg tatacgatgg gggttagcgc
     8461 cagattttaa cgtaacagtc acaattgaaa ccattgaata acaacagttg tggcgatagt
     8521 gggtgctaac ttaccaaata ataaatttgg tgaataattg tcgcgtcatt cattcctgaa
     8581 ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccaa gcataaaatg
     8641 cacttcgtta tggttgtcgg cagagatttt tcctttttat tactgcagga atactgccat
     8701 gacaccttca aattatcagc gtacccgctg gctgacactc atcggtacta tcattaccca
     8761 gtttgcgctg gggtcggttt atacctggag cctgtttaat ggcgcgcttt ctgccaagct
     8821 ggatgcgccg gtaagccagg tcgctttctc tttcggcttg ttaagtctgg ggctggcaat
     8881 ttcgtcttct gttgcgggca aattgcagga acgttttggc gttaaacgcg tcaccgtggc
     8941 ttccggcatt ttgctgggat taggcttctt cctgaccgcg cattccaaca acctgatgat
     9001 gctgtggtta agcgccggtg tgctggtcgg tctggcggat ggcgcgggtt acctgctgac
     9061 gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat
     9121 cggttcttat ggtctgggta gcctgggttt caaatttatc gacacgcagc tgctcgaaac
     9181 ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga tgattgtttt
     9241 cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca atggtgtggt
     9301 ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt
     9361 aatgttcctg actgcctgca tgagcggcct gtacgtgatt ggggtagcga aagatatcgc
     9421 ccaaagtctg gcacaccttg atgtggtttc cgcagccaat gcagtaacgg ttatttccat
     9481 cgctaacctt tcaggtcgtc tggtgctggg cattctgtct gacaaaattg cccgtatccg
     9541 tgttatcacc attggtcagg tgatttcgct ggtgggtatg gcggccctgc tgtttgcacc
     9601 attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac
     9661 tattaccgtc tttccatcac tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa
     9721 ctacggtgtg atttacctcg gtttcggtat cggtagcatt tttggttcga ttatcgcctc
     9781 actgtttggc ggtttctatg tgaccttcta cgtgattttc gccctgctga ttctgtcatt
     9841 ggcgctttct acgacgattc gtcagccaga gcagaaaatg ttgcgtgagg cgcatggctc
     9901 cctttaatgc tcagccgcga gctactgggc ttcagcttcc gtgtgcatca aacgcatcaa
     9961 cgaaatgtat aaacaatgac tcgtaaaatt tgggccagtt aactggccct ttttactgct
    10021 ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg cttcatgcac
    10081 actctttccc cacacttttt cgctttgctg tggtctactt attcgcgcgt gtagatttta
    10141 cttatctgac tacctccgca cttttcccct gccgggcctg aaaagccgct aagcagggtg
    10201 ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat tacacagcag
    10261 aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc ggttttttat
    10321 cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcgtttc tgctgttgtt
    10381 gaactggccg ccaccgtact ggtgaccttc tttttactac gtcttctatc gctgtttggc
    10441 cgccgcagct ggcgtattct ggcgtcgctg gtggtgctct tttccgcagg tgccagctat
    10501 tacatgacct tccttaatgt ggtcattggt tatggcatca tcgcttccgt catgaccacc
    10561 gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt aatcgccgtt
    10621 agtgcattgc ctcttatcct tatctggaat aaccgctgtc gctacacctt gctccgacaa
    10681 ctgcgaaccc cagggcagcg tattcgcagc ctggtggtcg tcgtactggc gggtattatg
    10741 gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag ggcgaccggc
    10801 gttgatttgc cgagttatgg cggtgtcgta gcgaactctt atctgccatc aaactggctt
    10861 tctgcgttgg ggctgtatgc ctgggcgcgg gtcgatgaat cttccgataa taattcattg
    10921 cttaatccgg cgaagaagtt tacataccag gctccacaaa acgttgatga tacttatgtc
    10981 gtgtttatca tcggtgaaac cacgcgttgg gaccatatgg gtattttcgg ctatgagcgt
    11041 aataccacgc cgaaactggc ccaggagaaa aatctggcgg cgttccgtgg ttactcctgt
    11101 gataccgcaa ccaaactctc actgcgttgc atgtttgtac gtcagggggg cgcggaagat
    11161 aatccgcagc gcacattaaa agaacagaac attttcgcgg ttctgaagca gttaggattc
    11221 agttctgacc tctacgctat gcagagcgaa atgtggttct acagcaacac gatggcagac
    11281 aacattgctt atcgtgagca gattggtgcg gagccacgta atcgtggcaa gccggtagat
    11341 gatatgttgc tggtagacga aatgcagcaa tcgctagggc gcaacccgga tggtaagcat
    11401 ctgatcattc tgcataccaa aggttcgcat tttaactaca cccagcgtta tccgcgtagc
    11461 ttcgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa agcgcagatg
    11521 atcaactcct atgacaactc ggtgacctat gtggatcact ttatctccag cgtgattgat
    11581 caggttcgcg ataagaaagc gattgtgttc tacgcagctg accacggtga gtcaattaat
    11641 gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggagca gttccgcgta
    11701 ccgatgatgg tctggatgtc agataaatat ctggaaaatc cggccaatgc gcaggcgttt
    11761 gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc gtcacgtaga gctgtacgac
    11821 accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga aaacaacaac
    11881 tggtgtcaca tcccgcagac aaaagtggca gcggctaact aagcggcctg ctgactttct
    11941 cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg cgcagtaaga
    12001 tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg
    12061 tcggaggttc gaatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggtt
    12121 tttttgcatt tgtagctcat gaggatgaga acctccgggg gcagggaggc tcgactcgac
    12181 gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga
    12241 tgcggcgcga ccgccttatt cgaccaacac accacacatt ttcctgccaa ctttgcgccc
    12301 catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc
    12361 ttaaatcttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg
    12421 ttcatggtat taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat
    12481 aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta
    12541 gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt
    12601 ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat
    12661 tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagta
    12721 cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc
    12781 gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta
    12841 tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg
    12901 cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta
    12961 acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtcccg ctttataacc
    13021 gcctggttga atttaaaatc ggcaccaccg aagtgatccc gggcctcgct gaaaagtggg
    13081 aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg aagtggcacg
    13141 acaataaaga attcaaaccg acgcgcgaaa tgaacgccga tgacgtggtg ttctcgttcg
    13201 atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc tggcggcagc tacgaatact
    13261 tcgaaggtat gggcttgccg gagctgatca gcgaagtgaa aaaggtggac gataacaccg
    13321 ttcagtttgt gctgactcga ccggaagcgc cgttcctcgc tgacctggca atggacttcg
    13381 cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca ccggaaaaac
    13441 tggacctcaa cccaatcgga accggtccgt tccagttaca gcagtaccaa aaagattccc
    13501 gtatccgcta caaagcgttt gatggctact ggggcaccaa accgcagatc gatacgctgg
    13561 tcttctctat tacccctgat gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc
    13621 aggtgatgcc gtacccgaac ccggcagaca tcgctcgcat gaagcaggat aaatccatca
    13681 acctgatgga aatgccgggg ctgaacgtcg gctacctctc gtataacgtg cagaaaaaac
    13741 cgctggatga cgtgaaagtt cgccaagcac taacctacgc ggtgaacaaa gacgcgatca
    13801 tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt
    13861 ggggctataa cgacgacgtt caggattaca cctacgatcc tgaaaaagcg aaagccttgc
    13921 tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg ccggtacaac
    13981 gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag
    14041 tgggcgtgca ggccaaaatc gtcacctacg aatggggtga gtacctcaag cgtgcgaaag
    14101 atggcgaaca tcagacggta atgatgggct ggactggcga taacggggac ccggataact
    14161 tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ttctaactac tcaaaatggt
    14221 gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cataacaaac
    14281 gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg gcactgatca
    14341 tcgctcactc caccgtgttt gaaccggtac gcaaagaagt caaaggctat gtggttgatc
    14401 cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc atacaagact
    14461 gatggcaaag gcaaaactgc ctgatgtgct ccgcttatca ggcctacgaa aattctgcaa
    14521 tgtattgaat ttgcacgatt ttgtaggccg gataaggcgt taacgccgca tccggcataa
    14581 acaaagcgca ctttgtcaac aatctgtata cccggtggcg ctgtgcctct gcgtgcagcg
    14641 ccatccggca gcaatacttc attctctgtc cgacaagggc gggaattgat ttgtgagcaa
    14701 tacagacacg cagttccagg ctgcgagtca ctatagagaa tccgggttat gttgcagttt
    14761 attctccgac gtttgggact cgtcatcccc acgtttatcg gtattaccct tctcacattt
    14821 gcctttgtcc acatgatccc gggtgatccg gtgatgatca tggcaggcga acgtgggatc
    14881 tcccctgagc gtcacgtgca gctgctggct gaactcggct tagataaacc gatgtggcag
    14941 cagtatctcc attacatttg gggcgtaatg cacggcgatc taggcatttc aatgaaaagc
    15001 cgaattccag tatgggaaga gttcgtgccg cgctttcagg ccacgctgga acttggcgtc
    15061 tgcgcgatga tttttgctac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa
    15121 cgcggttcca ttttcgatca cactgcagtt ggcctggcgc tgaccggcta ctcgatgccg
    15181 atcttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgccc
    15241 gtctccggtc gcgtgagcga tatggtgttc ctcgatgact ccaatccgtt aaccggtttt
    15301 atgctaatcg acaccgccat ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat
    15361 atgatcttgc ccgccattgt gctgggtact attccgctag cggtcattgt gcgtatgaca
    15421 cgctcctcga tgctggaagt gctgggcgag gattacatcc gcaccgcgcg cgccaaaggg
    15481 ctaacccgca tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct gccggtagtg
    15541 accgttatcg gcctccaggt gggaacattg ctggcggggg cgattctgac cgaaaccatc
    15601 ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg cgactatccg
    15661 gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cttgctggtc
    15721 gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg
    15781 tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag
    15841 ttctggcact attttaaacg caacaaaggc gcggtcgtcg ggctggttta cgtcgccatc
    15901 gtgctgttca tcgcgatctt tgccaactgg attgcgcctt ataacccggc ggatcagttc
    15961 cgcgatgcgc tgctcgcccc gccagcctgg caggaaggcg gcagtatggc acacttgctg
    16021 ggcaccgatg acgtcggtcg tgatgtgctg tcgcggctga tgtacggcgc gcgtttatcg
    16081 ctgctggttg gctgtctggt ggtggtgttg tcgctgatta tgggcgttat tctcggcctg
    16141 atcgccggtt acttcggcgg cctggttgat aacattatca tgcgcgtggt cgatatcatg
    16201 ctggcgctgc caagtctgct gttggcactg gtgctggtgg cgattttcgg cccgtcgatt
    16261 ggtaacgccg cgctggcgct gaccttcgtt gccttgccgc actatgtgcg cttaacccgc
    16321 gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg cctctcgcgt ggcaggtgcc
    16381 ggggcgatgc gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt
    16441 caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg tttcctcggc
    16501 atgggggcac agccgccaac gcctgagtgg ggcaccatgc tctccgacgt gttgcagttc
    16561 gcgcaaagcg cctggtgggt cgtgaccttc cctggtctgg cgatcctgtt gacggtgctg
    16621 gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact aaagcagtaa
    16681 gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag
    16741 cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggcg aagtggtcgg
    16801 gattgtgggt gagtccggct ccggtaagtc ggtcagttca ctggcgatta tggggctgat
    16861 tgattatccg ggccgcgtaa tggcagaaaa actggagttt aacggccagg atttgcagcg
    16921 tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga tcttccagga
    16981 cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa
    17041 agtgcatcag ggcggcaaca aaagtacccg ccgtcagcgg gcgattgacc tgctgaatca
    17101 ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac ccgcatcagc tttccggcgg
    17161 catgagccag cgcgtgatga tcgccatggc gattgcctgt cggccaaaac tgctgattgc
    17221 cgatgaaccg accaccgcgc tggacgtgac cattcaggcg caaatcatcg aactactgct
    17281 ggagctacag cagaaagaga acatggcgct ggtgttaatt acccatgacc tggcgctggt
    17341 ggcggaagct gcgcataaaa tcattgtgat gtatgcaggt caggtagtag aaacgggcga
    17401 tgcacacgcc atcttccatg cgccgcgtca cccgtatact caggcattgc tgcgtgcgct
    17461 gccagaattt gctcaggaca aagaacgtct ggcgtcgttg ccaggtgttg ttcccggcaa
    17521 gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg
    17581 tcgcgctgaa gaaccggcgc tgaatatgct cgctgatggg cgtcagtcca aatgccatta
    17641 cccacttgat gatgccggga ggcccacact atgagtacgc aagaggccac ctcgcaacaa
    17701 ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg catgttcgtg
    17761 ccggaacgtc tggtgaaggc gctggacggc gtttcgttta accttgaacg tggcaaaacg
    17821 ctggcagtag tgggcgaatc tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg
    17881 atcgaaacgc ccaccggcgg cgagctgtat tatcaggggc aggatctgct caagcacgat
    17941 ccgcaggcgc agaagctgcg tcggcagaaa atccagatcg tcttccagaa tccctacggt
    18001 tcgctgaatc cgcgtaagaa agttgggcaa attctcgaag agccgctgct tattaacacc
    18061 agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcaaa agtcggcctg
    18121 aaaaccgagc actatgaccg ctatccgcac atgttttccg gcggacaacg tcagcgtatc
    18181 gctattgccc gtggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtttct
    18241 gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt gcagcaggag
    18301 ttggggcttt cttatgtctt tatctcccac gacctgtcgg ttgtggagca cattgctgat
    18361 gaagtgatgg tgatgtacct aggccgctgc gtggagaaag gaacgaagga ccaaatcttc
    18421 aataacccgc gccatccgta cacccaggcg ctgctctccg cgacgccacg cctgaacccg
    18481 gacgatcgtc gtgaacgcat caaactcacc ggtgaactgc caagcccgct gaatcctccg
    18541 ccgggttgcg ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag
    18601 ccgcagctaa aagactacgg aggtcaactg gtagcttgtt ttgctgttga tcaggatgaa
    18661 aatccgcagc gttaactcac aaaaccgggg ccatgcgctc cggtttactc aatcagcttc
    18721 aacagtggcg acaagcatag caaaatgccg taaagcagaa tcagccaggc tttaaatccg
    18781 cgaagttttt ccagttgtgc gactttatag atcaggaaaa acggaatgag gcacgacaca
    18841 atgccatata acgggcttcc caactgaaag aacaccagca ctgatacacg aaacgaaacc
    18901 caaatcgtca acgtaatgac gataaaagcg cagatcgcca gagtcagcat gcgtgagtta
    18961 attttcttgg tatcaataat tcggcttaac agattgagaa taatgccttt aatagcctcg
    19021 tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg caaagatatt cagcaccgta
    19081 gaggtgatat gaatgatatg tccagggatc acctgcgcgg ccagtgccag tgccgagata
    19141 ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca
    19201 aaaaacagga tcaccgcgat gagcgtgata taactaattc ggtgggtacg cagcgcgagc
    19261 cgggttgcca gtactttatc tgcttcccgt ttacgatagg caatattcat tgggttaagt
    19321 acctgaataa ataccgcaga aaagaagcaa aatggaatgg taagcaggac atcgcggaaa
    19381 aagacggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca
    19441 aaaccgaaca cgacaataat ccctactttg accaccacca ttggcccaga aatcttaaac
    19501 agtaatcgtt caccaccaga cgcaatcgct atcagtacgg cgaaaatagc gactttataa
    19561 agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg
    19621 tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata
    19681 accccgagga aaattcccca gttcttcccc aggtaatgac tgataatatc ggtgtagtca
    19741 ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgcactac ccaggtgaca
    19801 ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact
    19861 ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag
    19921 tcgtagcgtg taaacggcaa tttctgattg tgtttcgata gtgtgttgtg ctgcataaag
    19981 tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac
    20041 aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt
    20101 cgcgaggagg cgcaattcta caaaattgcg catttcacta ccagtctgga gtcagcccgg
    20161 atatgaactg caactattgc aaataaagaa attaagctgg aaggcatcgc gcaatgagga
    20221 agattgccgg gtgctgacag taaaaagcaa tcgtagaatc agcgtcaagc cggaaggatt
    20281 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    20341 aaacccccgc acgttgcagg tatgtacctg acaacataac gggggctaat cttgaaccta
    20401 gaccactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc
    20461 gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
    20521 gccagtatga tcgtgaactg gctgaacaag cggaagtagc gtgtcatgtg ggcgtcaggc
    20581 tgccgtaatg gcaatttgcg cccggaccag gccgcagggg tgaaactctg cggccttttt
    20641 cgtaagaaaa atgcaacggt tgcaagcgga taatattctg cggaatgcgc agcacagagt
    20701 gaagatgaga cggattccgg aacagaaaaa cagtcgtaga atcagcgtca agccggaagg
    20761 attccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt tgctggagag
    20821 agaaaacccc cgcacgttgc aggtatgcac ctgacaacac cacgggggcc attcttgcat
    20881 ccaaacaata caagaatagc cgcgaaacgt tgtcattaca atacaggcgg ctatatgacg
    20941 ttcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggtatt
    21001 cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgccat gcgggcgtca
    21061 ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt
    21121 tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg cgcagcacag
    21181 agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg tcaagccgga
    21241 agggttccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
    21301 gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gccattcttg
    21361 aacctgaaca actcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg
    21421 acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc
    21481 attcttgcca gtatgatcgt gaactggctg aacaagcgga agtaacgtgt catgcgggcg
    21541 tcaggctgcc gtaatggcaa tttgcgcccg gaccaggccg caggggtgaa actctgcggc
    21601 ctttttcgta agaaaaatgc aacgattgca cgcggataat attctgcgga atgcgcagca
    21661 cagagtgaag acgtgacgga ttccggaaca gaaaaacagt cgtagaatca gcggcaagcc
    21721 ggaaaggttc cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct
    21781 ggagagagaa aacccccgca cgttgcaggt atgcacctga caacatcacg ggggctaatc
    21841 ttgaacccaa acaactcaag aatagccgcg aaacgttggc attacaacac aggcggctat
    21901 atgacgctcg cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct
    21961 ggcattcttg ccagtatgat cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg
    22021 gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg ccgcagaggt taaactctgc
    22081 ggcctttttc gtgcttactg cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac
    22141 ggtttatctt ggtattgaat aactactgca tttgagttct cggagaccgg tgctgtttgt
    22201 ggcaacccac tggtgagttt tttccagtca acattgtctt cggtgaaaat cttgccatca
    22261 agaacgcgaa ccaccagatc ggagatagcc aggaagctgc tcggttggtc aatgacaatc
    22321 ggtgccccct gatgtggtgc cttcatgccg aagaatttca ccccaacggg gacgtctgtg
    22381 atagacgggc tagggatatc acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg
    22441 ccgccgtgtt ccggcaccac gaccaccatc actttacgac ccgatttctc cagttcagta
    22501 aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg
    22561 ctgacccccg gataatggtt gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta
    22621 cggctgtttt tatctttttc ggtaacgtcc agccagcggt taagcacggc ggtatcgtca
    22681 taaaccggcg aaccatcaaa gcccagcaaa ataaccggca gatttgtttg atccatcaat
    22741 tcagtctgca tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta
    22801 tgtcccatca tcaggtgctg ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag
    22861 tcgttatttg ccggttgata cagattagtg tgcgaagtct gcccgcagct ggcgcgtagt
    22921 aaacggatcg ccgccgggcc actgtaggag gtggcggagt taaagttctt gaaatcaata
    22981 tcgaaatgcg accacagtgg atgcgacatc aacccggcgg cttctatatc cgaccaggaa
    23041 agcgaacaga tgttaatcac cagtagttca aatggctgag catcagcggg cagcgaagac
    23101 gggaaggtcg atttacgttt cgcctccgcg ttatagaaat tattcagcca ggcgttaagg
    23161 ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc
    23221 gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt
    23281 ccggctggcc acaaggagaa acttggtccc gccagggtaa gtacgttcag ccatagcagt
    23341 atggcaacca caaaaacggt aatgcgaatc cattgtgaca ggaataacca ggccactaat
    23401 aaaacaaaaa tggccccaat catctgccag ttaataaagc gtgtgacaag gtcgattaaa
    23461 taatcggtac tgaaccccgc cacctgcgaa ccctggctca ttatgctttc cgggccaggc
    23521 aaccagatgt catgccagaa caaagcaaag ccgatcggca gggcaatcca gtggcgcaag
    23581 cgatgcaggc tgtaacgcgg aatgggcatc agcagaaacg cggcaaacac caaattgagg
    23641 agcgggtgga agttaagata tcccgcccac aacaggccaa acttaaccag aaaatagaag
    23701 ttccagccgg aaaggccgcg ccagtattgc cagagggaag aaggcatggc ggtattttgc
    23761 gtaaattgag tcattttttg gttgccctgg ctttttccgt gcggcgtaac gtcccggccg
    23821 gtttaacata acgaggttta gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag
    23881 agtgccgcgc cagatagccc agcgggaaaa atatcagtgc gcaaacaata ataatttcaa
    23941 tgatatcgct gatggtcatc atgatgagcg ctccacagca tcatctaaca gtcgcatggg
    24001 ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg gtttagaact
    24061 ttgcgttaaa ggcagcggca ttccccattg ttctggggca agcaggcgca tctgcaccag
    24121 ctcggcactg atttgatcat cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt
    24181 ag
//
LOCUS       DAECOD010000056        24180 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.056, whole genome shotgun sequence.
ACCESSION   DAECOD010000056 DAECOD010000000
VERSION     DAECOD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.056"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(22..594)
                     /locus_tag="KKO15_002314"
     CDS             complement(22..594)
                     /locus_tag="KKO15_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBD6361082.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="KKO15_002315"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="KKO15_002315"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBD6361083.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="KKO15_002316"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="KKO15_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBD6361084.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="KKO15_002317"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="KKO15_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBD6361085.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="KKO15_002318"
     CDS             complement(3556..5337)
                     /locus_tag="KKO15_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD6361086.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="KKO15_002319"
     CDS             complement(5330..7102)
                     /locus_tag="KKO15_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD6361087.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="KKO15_002320"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="KKO15_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBD6361088.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="KKO15_002321"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="KKO15_002321"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBD6361089.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHLDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8661..10353
                     /locus_tag="KKO15_002322"
                     /pseudo
     CDS             8661..10353
                     /locus_tag="KKO15_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="KKO15_002323"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="KKO15_002323"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBD6361090.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="KKO15_002324"
     CDS             complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="KKO15_002324"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBD6361091.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="KKO15_002325"
     CDS             complement(11657..12028)
                     /locus_tag="KKO15_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBD6361092.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="KKO15_002326"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="KKO15_002326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD6361093.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="KKO15_002327"
     CDS             complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="KKO15_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBD6361094.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="KKO15_002328"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="KKO15_002328"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBD6361095.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="KKO15_002329"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="KKO15_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBD6361096.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="KKO15_002330"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="KKO15_002330"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBD6361097.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="KKO15_002331"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="KKO15_002331"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBD6361098.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="KKO15_002332"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="KKO15_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBD6361099.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="KKO15_002333"
     CDS             21799..22377
                     /locus_tag="KKO15_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6361100.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..23896
                     /gene="ampG"
                     /locus_tag="KKO15_002334"
     CDS             22421..23896
                     /gene="ampG"
                     /locus_tag="KKO15_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBD6361101.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5669 a         6664 c         5824 g         6023 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacaaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccacgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcagacgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcaagatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgccag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcaa cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctaacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt
    22801 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    22861 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc
    22921 agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg
    22981 tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct
    23041 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    23101 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    23161 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct
    23221 tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc
    23281 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    23341 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    23401 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    23461 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta
    23521 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    23581 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    23641 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    23701 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac
    23761 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    23821 tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg cgctacgaaa
    23881 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    23941 tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt
    24001 ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt
    24061 ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt
    24121 tatttcgcat tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta
//
LOCUS       DAECOD010000057        22968 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.057, whole genome shotgun sequence.
ACCESSION   DAECOD010000057 DAECOD010000000
VERSION     DAECOD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.057"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(208..471)
                     /gene="cedA"
                     /locus_tag="KKO15_002335"
     CDS             complement(208..471)
                     /gene="cedA"
                     /locus_tag="KKO15_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBD6361102.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            654..2915
                     /gene="katE"
                     /locus_tag="KKO15_002336"
     CDS             654..2915
                     /gene="katE"
                     /locus_tag="KKO15_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBD6361103.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(2962..3720)
                     /gene="chbG"
                     /locus_tag="KKO15_002337"
     CDS             complement(2962..3720)
                     /gene="chbG"
                     /locus_tag="KKO15_002337"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBD6361104.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3733..5085)
                     /gene="celF"
                     /locus_tag="KKO15_002338"
                     /gene_synonym="chbF"
     CDS             complement(3733..5085)
                     /gene="celF"
                     /locus_tag="KKO15_002338"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBD6361105.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKKPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5190..6032)
                     /gene="chbR"
                     /locus_tag="KKO15_002339"
                     /pseudo
     CDS             complement(5190..6032)
                     /gene="chbR"
                     /locus_tag="KKO15_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(6040..6390)
                     /gene="chbA"
                     /locus_tag="KKO15_002340"
     CDS             complement(6040..6390)
                     /gene="chbA"
                     /locus_tag="KKO15_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBD6361106.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6449..7798)
                     /gene="chbC"
                     /locus_tag="KKO15_002341"
     CDS             complement(6449..7798)
                     /gene="chbC"
                     /locus_tag="KKO15_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBD6361107.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(7883..8203)
                     /gene="chbB"
                     /locus_tag="KKO15_002342"
     CDS             complement(7883..8203)
                     /gene="chbB"
                     /locus_tag="KKO15_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBD6361108.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8502..8840)
                     /gene="osmE"
                     /locus_tag="KKO15_002343"
     CDS             complement(8502..8840)
                     /gene="osmE"
                     /locus_tag="KKO15_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBD6361109.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9042..9869
                     /gene="nadE"
                     /locus_tag="KKO15_002344"
     CDS             9042..9869
                     /gene="nadE"
                     /locus_tag="KKO15_002344"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBD6361110.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            10099..10986
                     /gene="cho"
                     /locus_tag="KKO15_002345"
     CDS             10099..10986
                     /gene="cho"
                     /locus_tag="KKO15_002345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBD6361111.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPTGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10946..11521)
                     /gene="ves"
                     /locus_tag="KKO15_002346"
     CDS             complement(10946..11521)
                     /gene="ves"
                     /locus_tag="KKO15_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBD6361112.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11724..12209)
                     /gene="spy"
                     /locus_tag="KKO15_002347"
     CDS             complement(11724..12209)
                     /gene="spy"
                     /locus_tag="KKO15_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBD6361113.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12539..13507)
                     /gene="astE"
                     /locus_tag="KKO15_002348"
     CDS             complement(12539..13507)
                     /gene="astE"
                     /locus_tag="KKO15_002348"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBD6361114.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13500..14833)
                     /gene="astB"
                     /locus_tag="KKO15_002349"
                     /pseudo
     CDS             complement(13500..14833)
                     /gene="astB"
                     /locus_tag="KKO15_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(14830..16308)
                     /gene="astD"
                     /locus_tag="KKO15_002350"
     CDS             complement(14830..16308)
                     /gene="astD"
                     /locus_tag="KKO15_002350"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBD6361115.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16305..17339)
                     /gene="astA"
                     /locus_tag="KKO15_002351"
     CDS             complement(16305..17339)
                     /gene="astA"
                     /locus_tag="KKO15_002351"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBD6361116.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17336..18556)
                     /gene="astC"
                     /locus_tag="KKO15_002352"
                     /pseudo
     CDS             complement(17336..18556)
                     /gene="astC"
                     /locus_tag="KKO15_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            19002..19808
                     /gene="xthA"
                     /locus_tag="KKO15_002353"
     CDS             19002..19808
                     /gene="xthA"
                     /locus_tag="KKO15_002353"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBD6361117.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19975..20684
                     /locus_tag="KKO15_002354"
                     /pseudo
     CDS             19975..20684
                     /locus_tag="KKO15_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            20689..21366
                     /gene="ydjY"
                     /locus_tag="KKO15_002355"
     CDS             20689..21366
                     /gene="ydjY"
                     /locus_tag="KKO15_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBD6361118.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21381..22088
                     /locus_tag="KKO15_002356"
     CDS             21381..22088
                     /locus_tag="KKO15_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD6361119.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            22088..22636
                     /locus_tag="KKO15_002357"
     CDS             22088..22636
                     /locus_tag="KKO15_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBD6361120.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22646..>22968
                     /locus_tag="KKO15_002358"
     CDS             22646..>22968
                     /locus_tag="KKO15_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD6361121.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         5802 a         6080 c         5488 g         5598 t
ORIGIN      
        1 aggatgaact atcaaaaata actcatcatg attagcaaaa cttaaccatt ttaaaataaa
       61 taaataatta aagaaaaaag atcacttatt tatagcaata gctcgtcaaa ggcagctttt
      121 tgttacaggt ggtttgaatg aatgtagcaa cgaaatgcag aatttcaggt cctgtaactc
      181 ccggcaaaac cgggaggtat gtaatcctta ctcagtcact tccccttcct ggcggatctg
      241 atttgcccaa cgttgggcag attcaggcac agtaaacgcc ggtgagcgca gaaatgactc
      301 tcccatcagt acaaacgcaa catatttgcc acgcagcatc cagacatcac gaaacgaatc
      361 catctttatc gcatgttctg gcggcgcggg ttccgtgcgt gggacatagc taataatctg
      421 gcggttttgc tggcggagcg gtttcttcat tactggcttc actaaacgca tattaaaaat
      481 tagaaaaact gtagtttagc cgatttagcc cctgtacgtc ccgctttgcg tgtatttcat
      541 aacaccgttt ccagaatagt ctccgaagcg ggatctggct ggtggtctat agttagagag
      601 ttttttgacc aaaacagcgg ccctttcagt aataaattaa ggagacgagt tcaatgtcgc
      661 aacataacga aaagaaccca catcagcacc agtcaccact acacgattcc agcgaagcga
      721 aaccggggat ggactcactg gcacctgagg acggatctca tcgtccagcg gctgaaccaa
      781 caccgccagg tgcacaacct accgcccctg ggagcctgaa agcccctgat acacgtaacg
      841 aaaaacttaa ttctctggaa gacgtacgca aaggcagtga aaattatgcg ctgaccacta
      901 atcagggcgt gcgcatcgcc gatgatcaaa actcactgcg tgccggtaac cgtggtccaa
      961 cgctgctgga agattttatt ctgcgcgaga aaatcaccca ctttgaccat gagcgcatcc
     1021 cggaacgtat tgttcatgca cgcggctcag cagctcacgg ttatttccag ccatataaaa
     1081 gcttaagcga tatcaccaaa gcggatttcc tctcagatcc gaacaaaatc accccagtat
     1141 ttgttaggtt ctctaccgtt cagggtggcg ctggctctgc cgataccgtg cgtgatatcc
     1201 gtggttttgc caccaagttc tatactgaag agggtatttt tgacctcgtt ggcaataaca
     1261 cgccaatctt ctttatccag gatgcgcata aattccccga ttttgttcat gcggtaaaac
     1321 cagaaccgca ctgggcaatt ccacaagggc aaagtgccca cgatactttc tgggattatg
     1381 tttctctgca acctgaaact ctgcacaacg tgatgtgggc gatgtcggat cgcggcatcc
     1441 cccgcagtta ccgcaccatg gaaggcttcg gtattcacac cttccgcctg attaatgccg
     1501 aagggaaggc aacgtttgta cgtttccact ggaaaccact ggcaggtaaa gcctcactcg
     1561 tttgggatga agcacaaaaa ctcaccggac gtgacccgga cttccaccgc cgcgagttgt
     1621 gggaagccat tgaagcaggc gattttccgg aatacgaact gggcttccag ttgattcctg
     1681 aagaagatga attcaagttc gacttcgatc ttctcgatcc aaccaaactg atcccggaag
     1741 aactggtgcc cgttcagcgt gtcggcaaaa tggtgctcaa tcgcaatccg gataacttct
     1801 ttgccgaaaa cgaacaagca gcattccatc caggtcatat tgttcctggt ctggacttca
     1861 ccaacgatcc cctgttgcag ggacgtttgt tctcctatac cgatacacaa atcagtcgtc
     1921 ttggtgggcc gaatttccat gagattccga ttaaccgccc gacctgccct taccataatt
     1981 tccagcgtga cggcatgcat cgtatgggga tcgacactaa cccggcgaat tatgaaccga
     2041 actcgatcaa cgataactgg ccgcgcgaaa caccaccggg gccgaaacgc ggcggttttg
     2101 aatcatacca agagcgcgtg gaaggcaata aagttcgcga gcgcagccca tcgtttggcg
     2161 aatattattc ccatccgcgt ctgttctggc taagtcagac gccattcgag cagcgccata
     2221 ttgtcgatgg tttcagtttt gagttaagca aagtcgttcg tccgtatatt cgtgagcgcg
     2281 ttgttgacca gctggcgcat attgatctca ctctggccca ggcggtggcg aaaaatctcg
     2341 gtatcgaact gactgacgac cagctgaata tcaccccacc tccggacgtc aacggtctga
     2401 aaaaggatcc atccttaagt ttgtacgcca ttcctgacgg tgatgtgaaa ggtcgcgtgg
     2461 tagcgatttt acttaatgat gaagtgagat cggcagacct tctggccatt ctcaaggcca
     2521 tgaaggccaa aggcgttcat gccaaactgc tctactcccg aatgggtgaa gtgactgcgg
     2581 atgacggtac ggtgttgcct atagccgcta cctttgccgg tgcaccttcg ctgacggtcg
     2641 atgcggtcat tgtcccttgc ggcaatatcg cggatatcgc tgacaacggc gatgccaact
     2701 actatctgat ggaagcctac aaacacctta aaccgattgc gctggcggga gacgcgcgca
     2761 agtttaaagc aacaatcaag gtagctgacc agggtgaaga agggattgtg gaagctgaca
     2821 gcaccgacgg tagttttatg gatgaactgt taacgctgat ggcagaacac cgcgtgtggt
     2881 cacgcattcc taagattgac aaaattccgg cgtaaatatg gataaggtca cgtcccctgg
     2941 ggagtgacct taccacctta cttacacatc aagataacta cccaaccgat aaccacgctc
     3001 agcaattgca tatttcaacg acgctgatgt cagcacttcc agctccgtta accgtgggaa
     3061 acagtaagca ctctgacgga tagtattatc gataaacgcc ggatgacaca tcacctccag
     3121 cgaaggatca ccccgatggc ttgcgtcatc cagcacttgc aggaacagtg cctcgctaat
     3181 ctcttcacca taaaacgcgc tactgaatcc ctgcgaactg cgcagattag ccggtaaatc
     3241 accagcgtta gatagcggct gacggtcgat gcgcaacgca atcccctctt cagccgcaaa
     3301 cctggcaaca atcgggaaaa tctgcgggaa catatgcaca tgatgatggc tatcaagatg
     3361 cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac tgactggcaa gctcctgagt
     3421 aatttcttcc agcggtaaag catcttcttc tgccaactgc cagatccatt ttcccagcac
     3481 accatcgcgg gttaaccccg gcatagctgt cagtggcttg cccatagtaa ggacaaagtg
     3541 catccctatg gccagactcg gttcatcacg actcaactgc accgcatggt caatagcctg
     3601 cccattcacc agcgccgtcg tcgatgtgac aatcccattg cgacaggcct cgataatgcc
     3661 gtagttctga cctttgctta agccaaaatc atcggcatta acaatcagta agcgttccat
     3721 aatcagcctc ggttaatgtg cttttttaag ctctgcgatg cagtcggcaa aatttggcag
     3781 ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc tcagcatcgc gatcggaatg
     3841 caccaacgga ctaaggttta gcgccagtaa cacatcgtta aattctccgc taagtgcggc
     3901 gttgctggca gcaatctcga agcctttaat ggtgtgaatc agccccatca ctttatcatc
     3961 gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc cccagcttac aggtcatttc
     4021 taccgcccag tctgccggaa tattatcaat atgcccatga tgcgggatat taacgtaatg
     4081 ttcagcttgc ttgtcgttgt agatagcgtt gatcacttcg cacgctgcat cagagtaata
     4141 agccccaccg cgctgttcca gttctttcgg cttaactttc aactccggtt ttttatacag
     4201 ctcaaaaagt tgtttctcga ctttctgtac tacctgcgct cgtgcgccgc ctttgtagta
     4261 ttcgcccatt tcaatagcca gcatctcttt ttgcttgaag taataaagca aataagaaca
     4321 cggcaacaga ttcagagaac gaattaagcc ttcgctaaat ggcagatcga aaatattttt
     4381 aacgccagat acttttaact gcccggatgc cacaccatca agcaattcgg caaagcgcga
     4441 cttgccattt accagcacat ccttaatgaa caccatatgg ttgaggccaa acaaatcgat
     4501 agataaatca tcgctgtctt tcagcatcag aacatcgtga ataaacatct tcatgccgat
     4561 cggaatatta cacacgccga taaaacgttt aaatccggta tgacgataaa cggcttcagt
     4621 aaccattccc gccgggttag tgaagttaat cacccatgca ttcggacaaa gttcttcgac
     4681 atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga cctttaaaca aaccgcccgc
     4741 gccgttggtt tcctgaccaa gataaccatg acttaatgga atacgttcat ccagttcacg
     4801 cgccggtaat tggccaacgc gcagttgggt agtaacgaaa tcagcatctt tcaatgcttc
     4861 gcggcaatcc agcgttttat aaagcttcat cgggacgcca gcgttatcaa tcatccgttg
     4921 gcagagatcg aaaataatat ccagtttagc tttgccaccc tcgacatcca ccagccataa
     4981 ttcgctgacc ggcaattcgt gataacgctt aataaatcct tccagtaact ccggggtata
     5041 gctgctcccg ccaccaatag tgacgacttt taatttctgg ctcataattt ctcccttcag
     5101 tacagaatac tgatatctgg catatctgcc cccccggaca taaataatcc agcaacagga
     5161 cagatatgtg aattgtcagg tataacgact tactgattaa attcagtcaa tttcttacga
     5221 tatctcttag gcgtaaagga cgttaatttc ttaaacgttt taataaacaa actcgggcta
     5281 ctataaccgg cctcaaacgc aatatccgtc acggaatagt tggtcatttc cagttgtttt
     5341 tttgcaaaat taatacggat ctcattaata atctgcattg gtgttttgcc ataatatcgt
     5401 tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca acgtcaccat attctccagc
     5461 gccgattcac taaactgctc tttatcatgc atcttttcta ccgtactttt cagccactac
     5521 ggtacatcat caatcacctg ttcttcgcgg taatgacgta aacggttaat gacataaaaa
     5581 gtaaccatct caacaaactc ttccagccct gtttcgcgga aatttaatga agaaatcact
     5641 gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc aacaaagcaa
     5701 taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc aacgttcaat
     5761 atgcgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg aataaaaaca
     5821 aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctagaaata acgcccggtt
     5881 aatactagag taaattcata atagtcgtgc tgatgcagtc cgctgatact ctcagttttg
     5941 ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg ggcagtggca
     6001 atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca gtttttcatg
     6061 aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta aatgatcctg
     6121 agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc cttcaatcag
     6181 tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca tcatggcttt
     6241 cgctgcggca aaatcgccct gcttcgcctg tttcaatgcg gcatacgcca ggctacgcgc
     6301 ttgtccagag ttgatgataa gccccatcac cacttcttcc agctcttcag cttctgtttg
     6361 cgtatcagga atattatcga gatccatcat acatcgttcc tctctcttct taccggcaag
     6421 attacccgta ccggcatcga ttaaaatttc aggggttagc gatatcttct tcgctctctt
     6481 ctttatcaat cgcattttgt gctttgttag ccaccacaac aaagggcaga taaattaacg
     6541 ttgcgatgcc aaggttgaag agtgcgacca gcaatgcggc gacgctaccg ttggtgttaa
     6601 agaaggctcc cagaccggtt ggcatggtcc acggtgcaat attggtcacc ggaggaatga
     6661 tgcccatgta gtacgctgcg agggtgattg ccgccagaat cggttgtacc agtacaaacg
     6721 ggataaacat caccgggttc atgataattg gcagaccaaa cagaatcggt tcgttaatct
     6781 ggaagatgcc ggacggcagc gccagttttg ccacctgacg ataatcagca cggcgagagg
     6841 cgataaagat agccaggatc aggcctaaag tcgcaccact gcccccaagg aaaataaagg
     6901 agtccagcat cggcttggcc cagatatgga aggtcttacc ggctgccagc gccgcttcga
     6961 cggaaccata ttgctgatag gtcgcgatat tttccagcgc ccacggcgtc ataatgccgt
     7021 tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa gaaccagagc agtggaacaa
     7081 agatcacata ggcccagccc accacgctac ccaacgatgc cagtggggtt gagatggtat
     7141 ccataatgat ctgatggaag ttggtgcccc aggtattcaa cgcccaggca ataatcccca
     7201 tcacggaaag aataataaag ccgggaatta atgccgagaa agaacgcgat actgaagcag
     7261 gtacgctgtc gggcagttta atgacccaat tgcggcggac aataaaggta aacatttctg
     7321 ccaccaccaa gccaataata atcccggaga tgatattcgc cccacctaac cagtttgcgc
     7381 caaccgcata ggcctcaccg acactatatg gggtgacggt cataaatgct gcaacggata
     7441 acaacccagc cgccagcgca tcgactttac gctcttctgc cagcgccatg ccaataaaga
     7501 acggtgccat taaagacatt attcctaatg ttccgttata tacgttgccg ccaatacctt
     7561 tcagaccatt aagtgtttca atggttgagg cgtcgaggcg aatacctaag gaataaaaaa
     7621 acgacccctc cccaaagctt agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa
     7681 gggttaacgg cattaagcga ataaagccat ttttgattgc attaacgtgt ggctgctttc
     7741 ctattttaac tgcaaaaggg aggagtacct tttcaagcga tgcaataaca ttactcatag
     7801 aaaaataccc ttaaaaaccg caatttaaat attacggtat tgatttatga aataactctt
     7861 tgacgggaaa atttaaaata aattaatttg ctgcggcttt tttaatcgct gcaaccgcag
     7921 ccttaagcac gcctaaacca tcgactttgc cataaagcag cgagtcaatt acttcaaccg
     7981 gtttgttggg taacaaacgc tggatttcgg gcaacatata agcaatctgc ggccctaata
     8041 acacgacatc ggcattctga cctttttcac cagccagtgt ttccggaaat gcttcaataa
     8101 tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt tgataccagt aaagagatag
     8161 acatgcccgc agaacaaaac agataaatgt gtttcttttc cataaaactg ccctcatcga
     8221 cgattatctg tcagccagac actccgcaag ccttgacctg cttccatgct ctgggtaact
     8281 tgcgaaacca acatgacttt ttttgtggct gaaaggagta tacggtatag acctgagcaa
     8341 tagtatttct ttgaccttat aaattgtttc tccttgacct gtggttatga ccctcttcac
     8401 atttcgggca aataattcgc gaagataatt aagaactcag gccaaaaaaa accggcgcaa
     8461 tggccggttt ccgttgttac tcaagctttc agacgaattg attacttcgc agcctgtgga
     8521 tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct
     8581 aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa
     8641 gtaccgcgag catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc
     8701 tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg
     8761 gtacgatcat aagctgtaca acccgccagc atggttaata ccgccgctgc actcagaatt
     8821 cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg
     8881 aatacattgg gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct
     8941 gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt
     9001 agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa
     9061 taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg
     9121 tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga
     9181 tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc
     9241 tgcgccagga aactcgcaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg
     9301 ttcaggctga cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat
     9361 taaccgttaa tatcaagggc gcggtattgg ctagcgagca ggcattgcgg gaagcaggca
     9421 ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat
     9481 atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca
     9541 ttaccggatt cttcactaaa tatggtgacg gcggtacgga tattaatccg ctgtatcgtc
     9601 tcaacaaacg ccagggtaaa cagttactga cggcattagg ttgcccggaa cacctttata
     9661 agaaagcccc aactgccgat cttgaagatg atcgcccttc tctgccagat gaagtggcac
     9721 tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaaccta cctgaacagg
     9781 tcgccagaac aatagagaac tggtatctga aaaccgaaca taaacgccgt ccgccaatta
     9841 acgttttcga tgatttctgg aaaaagtaat aatttgcacc tattggattg tgcgaaaaag
     9901 agtaatttgt tcacgccgga tgcggcgtga acgccttatt cgacctacaa aactatgcaa
     9961 attcaataca ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag acggtgtaac
    10021 ctttgtcatc aacccgcctc ttttttaatc gcctcccgcc tgttacactg gatagataac
    10081 cagcattcgg agtcaacagt ggtacggcgt ttaacttctc cgcggctcga atttgaagct
    10141 gcggcaattt atgaatatcc cgaacattta cattcattcc ttaatgactt acccacccga
    10201 ccaggggtgt atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc
    10261 gttaatatcc gcagtcgggt gctttctcat ttacgcaccc cggatgaagc cgccatgcta
    10321 cggcaatccc ggcgaattag ctggatctgc accgcaggcg aaatcggcgc tctgctcctt
    10381 gaagcgcgat taatcaaaga acaacagccg ctgtttaata aacggttgcg ccgcaatcgc
    10441 cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg
    10501 gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa
    10561 gcattgcaga gcatcgctga tgaacaaaaa ctttgttatg gcctgttggg actggaaccg
    10621 ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc
    10681 ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga
    10741 gtggtgtgtt ggccttggca aggtgcggtg gcactgaaag aacagcaccc ggaaatgact
    10801 caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg
    10861 acaacgttaa ttcggacacc caccgggttt gatcacgacg gttataaaat tctttgtaag
    10921 ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc
    10981 agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc
    11041 catcgaacca gcacgcgccc tggtcggtag tgagcagttt gtcgcccagt tgccacgccc
    11101 cgttaatcac aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa
    11161 ttctgacttt cgccttacag acatccaggc gcgtcataat attgaaatcc atcgacatct
    11221 gccctgccgt cagtttcgct tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg
    11281 gttttaaggt atggttaaag cggtctgcgc tttcaaggag catctcgccg ccttccagca
    11341 acgtcactat cctttccatg ccgggaaaca aagaaaactc cccattcgcc gcgatggatg
    11401 caatgctggc acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg
    11461 tttcaccggc agcgtttcgc cacagattca ccgacatttt acgcatatca aagtattcca
    11521 tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg
    11581 gcaactcggg tgatttacgt tagtggtgat caggcttttt ttacaactgt ttgagaagag
    11641 aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg
    11701 tcttaagttt tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct
    11761 ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc
    11821 aggatgttgt aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt
    11881 tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaatg
    11941 atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct
    12001 ttcatgattt cgtggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc
    12061 atgtcctgat gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc gtcagccggt
    12121 gctgcggtag tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag
    12181 gcaacaaaca gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta
    12241 cggctttctt aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat
    12301 taatcagagc aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag
    12361 aaaaaaagaa aatttgatgg agagtgatga gagaatatta caacacgatg attttgcaga
    12421 gattatgaag aactataccg gatgactggt gataaataaa gcaaataacc aggattaatc
    12481 tgtattaatt tataagaaag caacttaata cccgcagaat gatttctgcg ggtaagtatt
    12541 agcttatttt ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac ggattaggga
    12601 ataacacata ctctacatca tgggttacgg taaaacgttc ctctccgtcc tgcgccagca
    12661 atgttccttt ctcaaacggc ataaaattca gcgtgtcact tgccatatgc atttcgaagg
    12721 acggcgagtg gcgagtaatt tgcgaaacca cccgataacg gagcggcggt gttctcacga
    12781 taccgacact ctcaccagat agcagcgcag caattgcgct ggcagttact gcaaactggc
    12841 gaagatcgtt ttgcccaaag ggcaacgctt tgccaagttc caacgtacag gccagcgcgc
    12901 caaaatgtct gacgctgaaa tgggtaaacg taccaccagg ctcctgatgg aacaccagcg
    12961 cctccagccc cgccgcaccc agccacgtca gaaatttctc gtcccaggga atgtcgcgtt
    13021 gcggtaatac accgaactgc ggatgcaagg agccacgaat tgcggtatgt agatcaaggt
    13081 gccaacgcac agattctttg ccctggtcat aaaaatcttc caggcactgt tccagttcgc
    13141 gcgcccgaca ggtttctccg ctttcagcaa atagctgcca acgaccgcca aacattcggt
    13201 tcatatcgct atggcaataa cgtttccctt gcttcagcgc aggaggattc ccgaggatca
    13261 ccagcaaccg ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca
    13321 gcatttccac aggtgccgtc tcattaccgt ggatccccgc agaaatcacc agcgcgcctt
    13381 gcggtggcgt taatggcgtc agttccagca caccatcgcc cagccagcgc cagcgaacgc
    13441 cgttgatttc tcgctcggtg ataaccggtt ttttacccgt taaggtcaga gcaagaaaat
    13501 tatccattgc cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattgg
    13561 ctcaatacat ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca
    13621 gcagtaagac gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta
    13681 tcgttcatca tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa taccacccgc
    13741 aaccgcaggc acgccgggcc gccgccattc gccatgcttt cacgtaagtc aaagactttt
    13801 agttcgctaa tcgggttgtc agcggcaagg agttcattga gataacccca tactccggcg
    13861 tgttcccgac actcctgagg cagcaccaac atcatggaac catcatcgcg gctcaacagt
    13921 tggctgttaa acagataggt agacaccgca tccgacacgg aaacctgagt tgccggaact
    13981 tctatcgcca taaaaccgtt cacccgcgtg ccagtaactg tgactggcga gcgaacgcct
    14041 gttggtggca aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc
    14101 tctggtcgat aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat
    14161 taagccttgc caccacctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc
    14221 gggtatcatt gccttcttct cgcccgtaga caaaaagtag catacccggt tcaccgtaat
    14281 gaccgccgag acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca
    14341 acgccgaatg gacgctaaat ttctcttcgt ccttaaaaat cgcttttaac agcgattcag
    14401 tgacgggcgc ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca
    14461 ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg
    14521 gcgaagcgga actgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta
    14581 cctgctcatc gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt
    14641 gcggcgggat cacggcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc
    14701 cctgttttgc cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat
    14761 taccaaacga caggcccgcg taatgatgcg tcagccctac cagcccgtcg aaattaactt
    14821 cccaggcgtt catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgtggcggg
    14881 caatgttaac gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata
    14941 ccaggcgctg gggcgatggt taccggaagc accaatgccg ccgaatgggg cggtactggc
    15001 agcaccagta agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg
    15061 atcgaatttt tcccgctcgg gggaaaccag accgcaaaag agtccgaagc gagtgttgtt
    15121 cgccatccga atcgcctcat caaaagtatc ataacgccag acgcgcaaaa gcggcccgaa
    15181 cacctcttca tccggcacac cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa
    15241 cgatgtccct gcttgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag
    15301 ttgctgccag gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa
    15361 cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagact
    15421 agcaagaaac gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca
    15481 gcgttgcccg gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc
    15541 cgccacctca tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc
    15601 cggctgaccg gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag
    15661 caaaccgtcg agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac
    15721 caggttcagc acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc
    15781 actccacggt gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg
    15841 aacgatatgt ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac
    15901 gccgtgcggg cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc
    15961 ggtacgaacg tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc
    16021 ggttgccgct tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcggc
    16081 tttattgctt tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga
    16141 gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc
    16201 ggcatcggca tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg
    16261 cgatgcgccc tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc
    16321 tctgcgcaca ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt
    16381 tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg
    16441 aaatggtgat aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc
    16501 ccttctgcca cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac
    16561 tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc
    16621 tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct
    16681 tcctgggata aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc
    16741 ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta
    16801 ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca
    16861 accactttgt cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac
    16921 agcaaatagc cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg
    16981 ctgctgccgg tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc
    17041 tcttttgagg cgtgaaccaa cgtgccgacg cgatagttgt accagggatc gttcagccca
    17101 accgccacct caatagcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg
    17161 aacacatagc cctgctcgct tttgggcagt tcaccttgcc aggttttgat tgccctttcg
    17221 atacgcgccg aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg
    17281 ctggcaagct gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg
    17341 atgaacctcg gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca
    17401 cctcttcttc gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca
    17461 gtaccatcac gcctgctttc gccgcttcca gagagatctg tttcgcttgc ccggcgtaat
    17521 cggcattcag tacacagcca atcaacaaac ctaagccgcg aacttcactg aacaaaccat
    17581 agcggtgatt aatagtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat
    17641 taagcatctc tggtgtgttg atgagctcca gcactttgcc tgctaccgcc gaggccagcg
    17701 gattaccgcc ataggtggtg ccatgagtgc caacggtcat cacgcgggcg cactcttcgg
    17761 ttgccaacaa cgcaccgacc gggaaaccgc cgcccagcgc tttggcggtg gttaacagat
    17821 cgggcgtcac gccgtagtgc atataggcat acagctcacc agtgcgcccg acgccggttt
    17881 gtacctcatc aaaaatcagc aaggcattgt ggcggtcaca caattcacgc agaccttgta
    17941 aaaacgcgtt gctggctggc accacaccgc cttcgcccta gatgggctcg acaatcaccg
    18001 cacaggtggc gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac
    18061 gaatatccgc cggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga
    18121 cagtaaacag cgtgcgacca tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc
    18181 tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg
    18241 caccggagtt acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca
    18301 gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt
    18361 cgttcagcgc ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac
    18421 ccgcgaagtc gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta
    18481 ccggtataaa gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg
    18541 taattggctg agacatagcg acctctacag taaataattc gttatttgta tgttaacaat
    18601 aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg
    18661 cataaacggc ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa
    18721 gtgcatataa agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg
    18781 ttatgcagaa gtaaaatata attctagaat tgtgatcatt gacgaaattt actggaaatt
    18841 actgcgccat tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca
    18901 tcattaacaa ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc
    18961 taccatccac gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta
    19021 atatcaacgg cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac
    19081 cggatgtgat tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg
    19141 tggcgaagct cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc
    19201 tgctgaccaa agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg
    19261 cgcagcggcg gattattatg gcggaaatcc cctcactgct gggtaatgtc accgtgatca
    19321 acggttactt cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaagcgc
    19381 agttttatca gaatctgcaa aactacctgg aaaccgaact caaacgtgat aatccggtac
    19441 tgattatggg cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa
    19501 accgtaagcg ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga
    19561 tggacaggct gatgagctgg ggggtggtcg ataccttccg ccatgctaat ccgcaaacag
    19621 cagatcgttt ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc
    19681 gcatcgacct gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg
    19741 actatgaaat tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc
    19801 gccgctaatt tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa
    19861 atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt
    19921 ttttagttgt ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaac
    19981 gctgagcgta actttctgtt tgcctgtctt atttttgcgc tggtcattta cgctatccac
    20041 gctttcggtt tattcgatct gctcaccgat ttaccccact tacagacact cattcgccag
    20101 agcggattgt tcggttatag cctctatatt ctgttattca tcaatgcccc ctctttctgt
    20161 taccaggaag catactggtg atcgctggag gaatagtttt tggcccgctc ttagggacac
    20221 aactctcatt aattgccgca acgctggcct cctcgtgctc attcctgctg gcgcgctggc
    20281 tgggacgtga tttactgctg aaatacgtgg gccatagcca taccttccag gccattgaaa
    20341 aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc ccgttgtttc
    20401 cttacaatat tcaaaattac gcttacggat tcaccaccat cgccttctgg ccttataccc
    20461 ttatttcggc actcacgacc ctgcccggta ttgttattta taccgtgatg gcaagcgatc
    20521 tcgccaatga aggcattacg ctgcgcttta ttttacaact ctgtctggcg ggcctggcgc
    20581 tgtttattct catccagctc gcaaaactct acgcccgaca caaacatgtg gatctgtctg
    20641 cttcgcgccg cagcccactt actcacccaa aaaatgaagg atagaacgat gtcgcaacat
    20701 tattcagtgt catggaaaaa aggactggct gcactctgct tactggctgt tgccgggctt
    20761 agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg aatacgacgg actttcgaac
    20821 agccaaccgc tgcgcgtcga tgccaataac catacggtaa ccatgctggt gcaaattaat
    20881 ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg ctccaacgga
    20941 cataaatcgc tgtttatggc ttatgcgccc ccgaaagcat tttatgaagc cctgaaagag
    21001 gcaggtggta cgccgggcga aaacatgacg atggataata aagaaacgac tcatgtcaca
    21061 ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaagcgta ttccttcgat
    21121 gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc gctttggcgg taatttaacg
    21181 gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg atagctgccc ggtcggcatc
    21241 gtcagcaatg caacatacac ttatggtgcg gtggaaaaac acggcgaagt gaaattcaaa
    21301 ggcaatgcct cagttctccc ggcggataac acgctggcaa cggttacctt taaaatcacc
    21361 gaataaagcc aagataaagg atgatgaaga tacaatcgcg taaaatctgg tactaccgta
    21421 taaccctcat catcctgttg ttcgctatgc tgctcgcatg ggcgctgctt cccggcgtcc
    21481 atgagtttat caatcgcagc gttgcggcgt ttgccgccgt ggaccaacag ggtatagaac
    21541 gctttattca gtcttacggc gcactggcgg cagttgtctc gttcttgttg atgattttac
    21601 aggccattgc tgcaccgcta cctgcgtttt tgatcacctt tgccaatgcg tcgctgtttg
    21661 gcgcgttctg gggcggcttg ctgtcgtgga ccagttcgat ggccggcgcg gcgctgtgct
    21721 tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa attaaccggc aaaaccgtgc
    21781 ttgacagtat ggacggcttt ttcactcgct acggcaaaca caccatcctg gtctgtcggt
    21841 tattgccttt tgtccctttc gatccaatca gctatgctgc cggtctgact tcaatacgtt
    21901 ttcgctcgtt ttttatcgcc accgggcttg gtcaattacc ggcgactatt gtttattcct
    21961 gggcaggcag catgttaaca ggcggtactt tctggtttgt caccgaactg tttattctgt
    22021 ttgccctgac cgtggtgatt tttatggcga agaaaatatg gcttgagcgc cagaagagga
    22081 atgactgatg ggtttaccgc cgcttagcaa aattcctttc attttacgtc cgcaggcgtg
    22141 gctgcatcgt cgccattacg gcgaggtgct aagcccgatt cgctggtggg ggcggatccc
    22201 gtttatcttt tatttggtgt caatgtttgt tgggtggctg gagcgcaaac gctcaccgct
    22261 cgatccggta gtgcgatcgc ttgtcagcgc gcgcattgcg caaatgtgcc tgtgtgagtt
    22321 ttgcgtggat atcaccagta tgaaagtcgc cgagcgtacc ggcagcaccg ataaactgct
    22381 ggcagtggct gactggcggc aaagcccgct ctttagcgat gaagaacggc tggcgctgga
    22441 gtacgccgaa gccgcaagcg tcacaccgcc aacagtcgat gatgccctgc gtacccgact
    22501 ggctgcgcat tttgacgctc aggcgctcac cgaactgacg gcattgatcg gcctgcaaaa
    22561 cctgtcggct cgttttaatt ctgccatgga cattcccgct caggggctgt gccgtattcc
    22621 ggaaaaacgt tcttaaggag ggaagatgcg ccattgtggg tggttgctgg gattgttatc
    22681 gctgttttct ttggcaacac atgccagtga ctggcaggaa attaaaaatg aggccaaagg
    22741 gcaaaccgtc tggtttaacg cctggggcgg cgataccgca attaaccgct atctcgactg
    22801 ggttagcggc gagatgaaaa cccattacgc tataaacctg aagattgttc gtctcgcgga
    22861 tgccgcagac gcggtgaagc gcattcagac cgaagctgct gccggtcgta aaacgggcgg
    22921 ctcggtggat ctgctctggg tgaacggcga aaacttccgc accttaaa
//
LOCUS       DAECOD010000058        22870 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.058, whole genome shotgun sequence.
ACCESSION   DAECOD010000058 DAECOD010000000
VERSION     DAECOD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.058"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="KKO15_002359"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="KKO15_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBD6361122.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KKO15_002360"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KKO15_002360"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBD6361123.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="KKO15_002361"
     CDS             1532..2479
                     /locus_tag="KKO15_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBD6361124.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="KKO15_002362"
     CDS             2549..3355
                     /locus_tag="KKO15_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6361125.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="KKO15_002363"
                     /pseudo
     CDS             3365..3772
                     /locus_tag="KKO15_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3772..4266
                     /locus_tag="KKO15_002364"
     CDS             3772..4266
                     /locus_tag="KKO15_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBD6361126.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="KKO15_002365"
     CDS             4332..5129
                     /locus_tag="KKO15_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBD6361127.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="KKO15_002366"
     CDS             5140..5928
                     /locus_tag="KKO15_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBD6361128.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="KKO15_002367"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="KKO15_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBD6361129.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="KKO15_002368"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="KKO15_002368"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBD6361130.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="KKO15_002369"
     CDS             complement(8371..10002)
                     /locus_tag="KKO15_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBD6361131.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KKO15_002370"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KKO15_002370"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBD6361132.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGVDIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDLNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="KKO15_002371"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="KKO15_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBD6361133.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15246..17430
                     /gene="espL4"
                     /locus_tag="KKO15_002372"
                     /pseudo
     CDS             15246..17430
                     /gene="espL4"
                     /locus_tag="KKO15_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17399..19136)
                     /gene="aceK"
                     /locus_tag="KKO15_002373"
                     /pseudo
     CDS             complement(17399..19136)
                     /gene="aceK"
                     /locus_tag="KKO15_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19320..20624)
                     /gene="aceA"
                     /locus_tag="KKO15_002374"
     CDS             complement(19320..20624)
                     /gene="aceA"
                     /locus_tag="KKO15_002374"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBD6361134.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20654..22255)
                     /gene="aceB"
                     /locus_tag="KKO15_002375"
     CDS             complement(20654..22255)
                     /gene="aceB"
                     /locus_tag="KKO15_002375"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBD6361135.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     YQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSLGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(22524..>22870)
                     /locus_tag="KKO15_002376"
     CDS             complement(22524..>22870)
                     /locus_tag="KKO15_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD6361136.1"
                     /translation="DFPAALIRDYTDLEILAETEEGDAYLFASKDKRIAFVTGHPEYD
                     AQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRASWRSHGNLLFTNWLNYYVYQIT
                     PYDLRHMNPTLD"
BASE COUNT         5634 a         5887 c         5748 g         5601 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggaaaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtagaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tggtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cgcacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggcggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcagg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccac
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gctcaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cacttaatta ttaacaaaat cgcaagatct cattccaccg
    15121 aaacttttac ggctgcatta tgagattgag cgatttatga tattttttta gttgtaatca
    15181 tattaaattt tttcactgtt ctgatagtta aaattcaaga catcaataaa caatgagata
    15241 tttaaatgat tactcgtatt cctcgtagtt ctttctctgc aaatattaat aatacagccc
    15301 agacaaatga acaccaaacc ctgagtgaat tgttttataa agaactcgag gataaatttt
    15361 ctggcaagga gctggcgacg cctctattaa aaagcttctc agagaactgt agacaaaatg
    15421 ttcgtcatat ctttagcaac aaggattttg tcattaaatt ttccacgtcc gtcttacaag
    15481 ctgataagaa agaaattacg ataattaata aaaacgaaaa cacgacactc actcaaacca
    15541 ttgctacaat atttgaagaa tacctaatgg aaattttacc tcaacgctca gacactcttg
    15601 ataaacaaga attaaaccta aaatcagata gaaaagaaaa agaattccca agaattaaac
    15661 ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt atagtatacc gacacattgc
    15721 tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc aagatgatta
    15781 ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc agactttggc
    15841 gttgatttcc agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt
    15901 tatgaaagca tattttgaca agatggaatc aaatggcatc agtgttatgg cagccatatt
    15961 actggtggat aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg
    16021 tacccattac gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaattat
    16081 gagcgaaagc aaagaggata ttaaacacta ttctctgatg gattttatga atgtagatta
    16141 tagcctcctg aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc
    16201 acttccgaaa gaacagctat tgatgttaaa aggaactgtg gatgaaataa cccctccatt
    16261 atcaccagca acgatgaatt tgctaatggc aattggtcag aatcaccaac ttacgcaact
    16321 gatgattcag ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa
    16381 tagtattaac ctccccggtt tatatttggc tataaattat ggcaatgcgg atatcgttga
    16441 gactattttc aattcattgt cagaaacagg atatgaaggg ttactctcga aaaaaaatct
    16501 catgcatatt ctggaggcaa aagataaaaa tggtttttct ggattatttt tagcgatatc
    16561 acgtaaggat aaaaatgttg taacctcgat tctgaacgtc ttacctaaac tggccacaac
    16621 acatcattta gataatgaac aggtgtataa attcctgagt gccaaaaata gaacgtccag
    16681 ccatgtttta taccatgtta tggcgaatgg tgatgccgac atgctgaaaa tttttttggt
    16741 cgcgttacct ttgttaattc gcacatgtca tttgactaaa gaacaggtac tcgatctcct
    16801 gaaggcaaag gatttttatg gttgcccaag actatacctg gcgatgcaaa atggacatag
    16861 cgatatcgtg aaagttattc tcgaagcatt gccctgtctg gcccaggaaa ttaacatttc
    16921 agcttccgat attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt
    16981 tatggccatg cagcgcggac atatgaacgt tattaatact atttttaacg cattacccac
    17041 tctttttaat acgtttaaat tcgataaaaa aatatgaagc ccctcctcct ggcaaataat
    17101 tctaatgaat atcccggttt gttttcagcg atacagcata aacaacaaaa cgttgtagag
    17161 acggtttatc ttgctttatc tgaccatgca cgcctgtttg gatttaccgc tgaagatatt
    17221 atggattttt ggcaacacaa agcgccacaa aaatactctg cctttgagtt ggcttttgaa
    17281 tttggtcacc gggttattgc tgaattaatc cttaatacat taaataagat ggctgaaagc
    17341 tttggcttta cggataaccc tcgatacatt gcggagaaaa attatatgga agctttactc
    17401 aaaaaagcat ctccccatac cgtacgctaa atctttgcct gcgccgatac gcataaacat
    17461 cttccacatg cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca
    17521 ggtcggcgtg catctcttca aacagcggac caatacgcgg gtcggcgcat agccagtggc
    17581 gaaactcttc cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt
    17641 cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa
    17701 tttcatcgta atcataaaaa accacacgcc cgtgacgggt gacaccgaag tttttaaaca
    17761 gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg aatagcgtta ccgtattctt
    17821 caatggcgtc gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca
    17881 tccgccgctc gattatagag atggcgaatt acaatctgct caccaagatc ggtgattttt
    17941 tccgctgctt cctggagcag taattccatt aataccgggg aaatatgccg cttctccagc
    18001 acaaagtttt caaactcctg ggtgtccgcc attcggccca cacggtcgtg ctctttcacc
    18061 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
    18121 ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa acaccaacat caccattcca
    18181 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
    18241 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg
    18301 gttttacctg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa
    18361 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg
    18421 caggtatcaa taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac
    18481 gtgccggaag gcgtgatcag tttgcccacc agccaggcgg ctttattgcg gtaaaacagt
    18541 tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga
    18601 tggcgaatga tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg
    18661 tcgctgataa cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc
    18721 agcgggcggg gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc
    18781 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
    18841 gcaatctcga agcgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt
    18901 aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga
    18961 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
    19021 tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
    19081 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
    19141 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
    19201 tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga
    19261 ttgccggatg cggcgtaaat gccttatccg gcctacagtc agcaacgttt gtaggttgct
    19321 tagaactgcg attcttcagt ggagccagtc agcgcggtga ctgaagacgt gccgccctga
    19381 ataatagtcg tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag
    19441 gtatagccat ctttcgcggc ggcaaattcc ggctgctgca ctttctcaac atagtgcttc
    19501 ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca tgttaaacca catgctgtgg
    19561 atacctgcca gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag
    19621 ctggcaatag ttttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa
    19681 gccagcagtt tgcccggata tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc
    19741 agatccggcg tggaggtttc acaccagact aggtcagcat atggcgcata cgccaggcca
    19801 cggctgatcg cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg
    19861 ccggtaataa attcgctgtc atacgggtcg caatctgagg tgatcagatc cgccgcatca
    19921 gcatcggtac gtgcaaccag cagggttgga acgcccgtca cgtcagctgc cagacgcgcc
    19981 gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa ctttgccgcc catgtgaccg
    20041 catttcttca ctgacgccag ctgatcttcg aagtgaactg ccgcagcacc ggcttcaatc
    20101 atcgctttca tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcatcggca
    20161 acgatcggca ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cacggaccat
    20221 tggatctgat cggcacgacg gaaggtgttg ttgatccgct ccaccacagc tggcaccgag
    20281 tttgccggat agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct
    20341 acctgccatc ccgacagata gactgcttca atacccgctt tcgcctgttg cagcgcctga
    20401 ccgccagtca gtgcaccgag gctgttgatg tagccttttt tcgactcacc gtgcagcaga
    20461 cgccacattt tcgctgcgcc cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt
    20521 aatttcacca catcttccgc actgtatggg cgagtaatgc cttcccaacg cggttgagtc
    20581 cactctttct gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata
    20641 gttatgtggt ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact
    20701 catcggaagt ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgg cccagggaga
    20761 aacgttcttc gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga
    20821 acaaggcttt ggtcaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc
    20881 agatcgaggt acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca
    20941 cgcagccgtt gccggagatc cacgcttcga tgtactgcac agccacgcga atgttagcgc
    21001 gcataccttc ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg
    21061 gcgcgtcttg ttcgcgcaat acttcaagct gatttttacg ggagccgaga atgtcgttga
    21121 ataccgccat tgccgtgtcc gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt
    21181 tattggcttc cagcgcttta tccgctttta ctttgttgag cacctggtta ttacgctctt
    21241 catctttgct cggaataaac gcggccatgc cgcccatcgc aaaagcaccg cgtttatggc
    21301 aggttttaat cagcaggcgt gagtaagcat tcaggaatgg tttatccatc gtcacggcct
    21361 gtctgtctgg caggacacga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt
    21421 aatcccagcg accgcagttc agaccaacaa tatggtcgcg cagcgcgtga aggatttcgt
    21481 ccatctggaa cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg
    21541 gcagattaaa gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgat
    21601 aggactgggt tttcggcaga tagaaatagg gaccgctgcc ctttgccaac agtgcctgat
    21661 agttgtggaa gaaatagagc gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc
    21721 aggtgacatg tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat
    21781 tgggcttgag ctgataaatt ttgcctgctt cattggtata gctgatggta ccgttaaccg
    21841 catcgcgcag gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt
    21901 cgaaatcggc cataaagact ttcacattgg cgttcagtgc gttgatcacc atcttgcgct
    21961 ctaccgggcc agttatctct acgcggcggt cttctaagtc cgcaggaatc ccgcgaattt
    22021 tccaatcagc atcgcgaatg gaagccgttt ccgaaataaa atcaggcaac gttccgttat
    22081 caatatcttg ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat
    22141 gcgtcaccag ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct
    22201 cgccatacgg ccttgtgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc
    22261 agctcctcgt catggatccg aaaacttccc ctctgtgaac gaaggaacgt tgtgcgcttt
    22321 tcgttcagca caactaagac tactcattta aaatgcaaaa tcaaaaacaa tttccatttt
    22381 aattaaaaat acttgataac ttattgataa caatatgaat aaaatttatt cacttgctga
    22441 ggtgcgtttc ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct
    22501 taacgatcga ctatcacaga agattaatcc agcgttggat tcatgtgccg tagatcgtat
    22561 ggcgtgatct gatagacgta atagttgagc cagttggtaa acagcaaatt accgtgacta
    22621 cgccagctcg ctcgcggttt gttttgcgga tcgttgtgcg ggaaatagtt atacggtaca
    22681 tccgggtcta gtccggcttc cacatcgcgt aaatattcct gcgccagcgt ttgcgcatca
    22741 tattcgggat ggcccgtcac aaaggcaatg cgcttatctt tgctggcaaa cagatatgca
    22801 tccccttctt ccgtctctgc cagaatttcc agatcggtgt aatcacgaat caacgctgcc
    22861 ggaaagtcag
//
LOCUS       DAECOD010000059        22402 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.059, whole genome shotgun sequence.
ACCESSION   DAECOD010000059 DAECOD010000000
VERSION     DAECOD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.059"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            82..2667
                     /locus_tag="KKO15_002377"
     CDS             82..2667
                     /locus_tag="KKO15_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBD6361137.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRS
                     SGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSL
                     KGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEAD
                     GSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTW
                     YGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLN
                     QLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGK
                     LQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRG
                     QPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETL
                     LDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGS
                     LVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRI
                     ALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIAT
                     LDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRM
                     DAICR"
     gene            2658..3641
                     /locus_tag="KKO15_002378"
     CDS             2658..3641
                     /locus_tag="KKO15_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6361138.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            3739..4281
                     /locus_tag="KKO15_002379"
     CDS             3739..4281
                     /locus_tag="KKO15_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6361139.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4289..4804
                     /locus_tag="KKO15_002380"
     CDS             4289..4804
                     /locus_tag="KKO15_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6361140.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTGFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4770..5508
                     /locus_tag="KKO15_002381"
                     /pseudo
     CDS             4770..5508
                     /locus_tag="KKO15_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            5619..6629
                     /gene="pyrD"
                     /locus_tag="KKO15_002382"
     CDS             5619..6629
                     /gene="pyrD"
                     /locus_tag="KKO15_002382"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBD6361141.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6803..7345
                     /gene="zapC"
                     /locus_tag="KKO15_002383"
     CDS             6803..7345
                     /gene="zapC"
                     /locus_tag="KKO15_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBD6361142.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7342..8451)
                     /gene="ycbX"
                     /locus_tag="KKO15_002384"
     CDS             complement(7342..8451)
                     /gene="ycbX"
                     /locus_tag="KKO15_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBD6361143.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8695..10803
                     /gene="rlmKL"
                     /locus_tag="KKO15_002385"
     CDS             8695..10803
                     /gene="rlmKL"
                     /locus_tag="KKO15_002385"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBD6361144.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10815..12722
                     /locus_tag="KKO15_002386"
     CDS             10815..12722
                     /locus_tag="KKO15_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6361145.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12852..14105
                     /gene="pqiA"
                     /locus_tag="KKO15_002387"
     CDS             12852..14105
                     /gene="pqiA"
                     /locus_tag="KKO15_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBD6361146.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14110..15750
                     /gene="pqiB"
                     /locus_tag="KKO15_002388"
     CDS             14110..15750
                     /gene="pqiB"
                     /locus_tag="KKO15_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBD6361147.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15747..16310
                     /gene="pqiC"
                     /locus_tag="KKO15_002389"
     CDS             15747..16310
                     /gene="pqiC"
                     /locus_tag="KKO15_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBD6361148.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16566..16733
                     /gene="rmf"
                     /locus_tag="KKO15_002390"
     CDS             16566..16733
                     /gene="rmf"
                     /locus_tag="KKO15_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBD6361149.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16803..17321)
                     /gene="fabA"
                     /locus_tag="KKO15_002391"
     CDS             complement(16803..17321)
                     /gene="fabA"
                     /locus_tag="KKO15_002391"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBD6361150.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17390..19150)
                     /locus_tag="KKO15_002392"
     CDS             complement(17390..19150)
                     /locus_tag="KKO15_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBD6361151.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19335..19787
                     /gene="matP"
                     /locus_tag="KKO15_002393"
     CDS             19335..19787
                     /gene="matP"
                     /locus_tag="KKO15_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBD6361152.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19863..20909)
                     /gene="ompA"
                     /locus_tag="KKO15_002394"
     CDS             complement(19863..20909)
                     /gene="ompA"
                     /locus_tag="KKO15_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBD6361153.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21266..21775)
                     /gene="sulA"
                     /locus_tag="KKO15_002395"
     CDS             complement(21266..21775)
                     /gene="sulA"
                     /locus_tag="KKO15_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBD6361154.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21994..>22402
                     /gene="sxy"
                     /locus_tag="KKO15_002396"
     CDS             21994..>22402
                     /gene="sxy"
                     /locus_tag="KKO15_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBD6361155.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5719 a         5255 c         5875 g         5553 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattta gcggggctaa cagtattcat agcagcgcag acagtgattc tggcgacagc
      661 tattttctga atttaaacag tggcgttaat ttaggcccat ggagattgcg caacaattca
      721 acatggagtc gcagtagtgg ccaaaccgca gaatggaaga atctcagcag ctatttgcag
      781 cgggcggtta ttccactaaa aggcgaactg accgtaggtg atgattatac tgcaggcgat
      841 tttttcgata gtgtcagctt tcgtggtgtg cagctggcgt cagatgacaa catgctgcca
      901 gacagcctga aagggtttgc gcctgtggtg cgtggtatcg ccaaaagcaa tgcccagata
      961 acgattaagc aaaatggtta caccatttac caaacttatg tatcgcctgg tgcttttgaa
     1021 attagtgatc tctattccac gtcgtcgagc ggtgatttgt tagttgaaat caaagaagcg
     1081 gacggcagcg tcaatagcta cagcgtaccg ttttccagcg tgccattact ccagcgtcag
     1141 gggcgaatca aatacgcggt gacactggcg aaatacagaa ccaatagtaa tgaacagcag
     1201 gagagcaaat ttgcccagac cacgttgcag tggggcggac cgtggggaac gacatggtat
     1261 ggtggtggac aatatgctga atattaccgt gccgccatgt ttggtctggg atttaacctt
     1321 ggcgatttcg gagcaatttc gttcgatgcg acccaggcga agagtacgct ggcagaccaa
     1381 agcgaacata aaggtcagtc atatcgtttt ctgtatgcca aaacgctcaa ccaattgggc
     1441 actaattttc aattgatggg ctatcgctat tcgacgtcgg gtttctacac cctttccgac
     1501 accatgtata aacatatgga tggctacgaa tttaatgacg gtgatgatga agatacgccg
     1561 atgtggtcgc gttattacaa tttgttttac accaaacgtg gcaaactgca ggtcaatatc
     1621 tcccagcaat taggcgagta cggttcgttt tatttaagtg gtagccagca aacttactgg
     1681 cataccgatc aacaggatcg gctattacag tttggctaca acacgcaaat taaagatctc
     1741 tcgctggggg tttcctggaa ctacagtaag tcccgtggtc aacctgatgc tgatcaggtg
     1801 tttgcactaa atttttccct gccgctcaat ctgttgctcc ccagaagtaa tgatagctat
     1861 accaggaaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac
     1921 attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc
     1981 gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac
     2041 aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa
     2101 caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat taccctgggg
     2161 caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa tactcgtgtg
     2221 gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact
     2281 tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt
     2341 gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat
     2401 gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt
     2461 ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc
     2521 tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct
     2581 cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt
     2641 acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac
     2701 tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat atcgggcagt
     2761 gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggatg
     2821 aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgaatg
     2881 attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg
     2941 ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg
     3001 ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg
     3061 tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg
     3121 gccagggggc tagcggtaat agcggtagct tttcactgaa aatattgcag ccgttcgttg
     3181 gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg
     3241 caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg tctggtacga
     3301 tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt gatctgggcg
     3361 acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag
     3421 aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac gtcaatctga
     3481 gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca cgtcctggcg
     3541 tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt gggacactac
     3601 cgctctctat tcctgatgat gcagacagta tccgcgaatg aatgtctatc cagtcagcac
     3661 gacaggcgta ccaccagaaa ccgggcgatt tgaagccacg gcaacggtga gaataaattt
     3721 tgattaacgc gtgaacgtat gaagaaaaaa acgatatatc agtgcgttat tttgttcttt
     3781 agccttctta acatccatgt cgggatggct gggcctgaac aagtcagtat gcatatttat
     3841 gggaatgtgg tcgatcaggg ctgtgatgtc gccaccaaaa gtgcattaca aaatattcat
     3901 attggtgatt ttaatatcag tgattttcag gccgcgaata ccgtaagcac tgctgctgat
     3961 ttgaatattg atatcaccgg ttgtgccgct ggtattactg gcgcggacgt cctttttagc
     4021 ggcgaggctg acacccttgc gccgacactg ctcaaactaa ctgacacagg cggaagcggt
     4081 ggtatggcaa cggggattgc cgtgcaaatt cttgatgcgc aaagtcagca agaaatcccg
     4141 ctcaatcagg tccagcctct tacgccctta aaagccgggg ataacacact caaatatcaa
     4201 cttcgttata agtccacaaa ggcgggagca acgggcggta atgcgacggc ggttctctat
     4261 tttgatctgg tttaccagtg aaggagtgat gttgaaacgg ataatctgga ttctgttctt
     4321 actgggatta acggggggct gtgagctatt tgcccatgat ggcacggtca acattagcgg
     4381 atcgtttcgc cgtaatacat gcgtgctggc acaggatagc aagcaaatca atgtgcagtt
     4441 aggcgatgtc tcgctgacag gtttttttca tgggaattat ggcccggaga aatctttcat
     4501 cattaatttg caggattgcg gaacagatgt cagcacggtt gatgtcacct tttcaggaac
     4561 ccccgacgga gtgcagagcg aaatgctgtc cattgaaagt ggtacggatg cagccagtgg
     4621 gctggcaatt gcgattctgg atgatgcgaa gatattgatt ccgcttaacc aggcgagtaa
     4681 agactacagc ctacatagcg gtaaggtgcc attaactttt tatgcgcaat tgcgacctgt
     4741 caatagcgat gtgcagtcgg gtaaagtgaa tgccagcgcg acatttgtac ttcattatga
     4801 ctaatacgtg gaacagatta gcgctcttga ttttcgccgt tttatcgctg ctggtggcgg
     4861 gtgaattgca ggcaggagtg gtggtcggcg gaacgcgatt tatctttccg gcagacagag
     4921 aatcgatatc tattttactg actaatacca gtcaggaatc ttggcttatt aacagcaaaa
     4981 tcaatcgccc aacgcgttgg gccggggggg gaagcgtcga cagtgccagc actattactg
     5041 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     5101 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     5161 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     5221 ctgttctggc gacccgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     5281 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     5341 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc gcctaaaagc
     5401 cagcgtcaga caaactggtg tcaggtaata gcacggtgtc atgtcgcctg gcggacgata
     5461 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttctga
     5521 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     5581 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     5641 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     5701 gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     5761 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     5821 gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     5881 cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     5941 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     6001 aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa gatacgccag
     6061 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     6121 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     6181 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     6241 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gacgaattga
     6301 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     6361 ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     6421 gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta gaattaaacg
     6481 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     6541 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     6601 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     6661 tcagggcttt attttcagcc ctggttgttt tatattcatc gttgttgctt atttagacat
     6721 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     6781 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     6841 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     6901 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt gtgttgatga
     6961 tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagacca
     7021 aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac cgcaaatgcc
     7081 gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     7141 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     7201 aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     7261 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga atgttgacag
     7321 cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg gtacacagct
     7381 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     7441 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     7501 aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac gaaatgcctg
     7561 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     7621 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     7681 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     7741 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     7801 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     7861 caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga
     7921 aaccaccaga ttggtgcgga attgctccat ttttacactg gccggacaac gttgttggag
     7981 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     8041 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     8101 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     8161 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     8221 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     8281 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     8341 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     8401 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     8461 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     8521 acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg ccctgggttg
     8581 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc catgtccaga
     8641 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
     8701 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaat
     8761 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
     8821 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
     8881 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
     8941 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
     9001 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
     9061 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
     9121 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
     9181 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
     9241 atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
     9301 ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt
     9361 tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
     9421 gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
     9481 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
     9541 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
     9601 tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga accggcgctg
     9661 attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg ctggaatctc
     9721 tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc agacaaacag
     9781 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
     9841 acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct gcgtaagaac
     9901 ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg cctgtatgac
     9961 gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    10021 gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct gttcgatatt
    10081 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    10141 gaacgccata agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    10201 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    10261 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    10321 ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc
    10381 agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg caacctgcgt
    10441 ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    10501 cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca
    10561 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
    10621 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    10681 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    10741 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    10801 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    10861 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    10921 gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    10981 atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa caggatccac
    11041 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    11101 aatatctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg agcgagaaaa
    11161 atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg tggcagctgg
    11221 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
    11281 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
    11341 gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc gactggctgg
    11401 aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    11461 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    11521 ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa gaactacaaa
    11581 atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgccag gggatcaaag
    11641 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt cgcgaacgtg
    11701 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    11761 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag caactggtga
    11821 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    11881 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcggac agcgggcgta
    11941 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    12001 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaatggca
    12061 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    12121 cgccagtacg tgcgttttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    12181 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    12241 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    12301 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
    12361 ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa tatgtggcac
    12421 tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca gaaactgtaa
    12481 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    12541 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg gatgcttcct
    12601 tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct gctgcagagc
    12661 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    12721 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    12781 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    12841 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    12901 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    12961 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg
    13021 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    13081 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    13141 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    13201 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    13261 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
    13321 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    13381 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    13441 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    13501 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    13561 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg cagtggacac
    13621 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    13681 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    13741 ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt atggtgccaa
    13801 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    13861 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    13921 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    13981 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    14041 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    14101 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    14161 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    14221 tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    14281 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    14341 gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
    14401 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    14461 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    14521 aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga tgcgaaaggt
    14581 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    14641 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    14701 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    14761 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    14821 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    14881 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    14941 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    15001 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    15061 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    15121 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    15181 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    15241 gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
    15301 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggctt ggcgcaaatc
    15361 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    15421 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    15481 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    15541 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    15601 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    15661 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    15721 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    15781 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    15841 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    15901 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    15961 tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc aacaccctgg
    16021 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    16081 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    16141 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    16201 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg gccggtgtct
    16261 ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa ttataaagat
    16321 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    16381 gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta gctcacaaat
    16441 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    16501 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    16561 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    16621 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgagt caaaggtcac
    16681 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    16741 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgatgat
    16801 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    16861 agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg acgattaaca
    16921 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    16981 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    17041 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    17101 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    17161 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc tatcatcagc
    17221 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg accagaggca
    17281 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    17341 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    17401 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    17461 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    17521 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    17581 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga acgttagcag
    17641 tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata gcaaagaagc
    17701 cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga tcgactgaac
    17761 ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc agggcgcaga
    17821 gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca aatgtcagcg
    17881 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    17941 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    18001 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    18061 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    18121 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    18181 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    18241 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    18301 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa gcatccgctc
    18361 ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactggctta
    18421 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    18481 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    18541 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    18601 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    18661 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    18721 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    18781 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    18841 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc agttggcctg
    18901 catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca gactcttccg
    18961 gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    19021 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    19081 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    19141 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    19201 cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa agctcacagg
    19261 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    19321 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    19381 agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata gaagccagtg
    19441 ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg
    19501 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag accattcggg
    19561 ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    19621 tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt aaaacgcttt
    19681 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    19741 tgtcttctct taagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    19801 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgata
    19861 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    19921 cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    19981 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    20041 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    20101 gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc tttcggatct
    20161 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    20221 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    20281 ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg gtaggaaaca
    20341 cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc accgatgttg
    20401 ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta ctcaacacca
    20461 cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc accaggtacg
    20521 ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat gtccagatcg
    20581 tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt gtatgcgccg
    20641 ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaaacca
    20701 acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt ttcgtgggtc
    20761 ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag tttagcacca
    20821 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    20881 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    20941 catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat
    21001 taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg
    21061 tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag
    21121 gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa accgcttcag
    21181 acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaaa
    21241 gttctaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg
    21301 aaagttgtct cgttgtgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt
    21361 taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgtca
    21421 accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt gactccactg
    21481 tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct
    21541 gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct
    21601 gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc
    21661 gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt
    21721 tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg tacataatca
    21781 atccagcccc tgtgagttac tgtatggatg tgcagtacat ccagtgacaa caaagatcaa
    21841 ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa
    21901 tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc
    21961 attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc
    22021 tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc
    22081 agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat
    22141 cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca
    22201 tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg
    22261 cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag
    22321 aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg
    22381 gaagcgattc tcggggaggt gg
//
LOCUS       DAECOD010000060        22281 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.060, whole genome shotgun sequence.
ACCESSION   DAECOD010000060 DAECOD010000000
VERSION     DAECOD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.060"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1028)
                     /locus_tag="KKO15_002397"
     CDS             complement(<1..1028)
                     /locus_tag="KKO15_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361156.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTEGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="KKO15_002398"
     CDS             complement(1130..2110)
                     /locus_tag="KKO15_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBD6361157.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="KKO15_002399"
     CDS             complement(2118..2828)
                     /locus_tag="KKO15_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBD6361158.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="KKO15_002400"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="KKO15_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBD6361159.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PVVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="KKO15_002401"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="KKO15_002401"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBD6361160.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="KKO15_002402"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="KKO15_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBD6361161.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="KKO15_002403"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="KKO15_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBD6361162.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="KKO15_002404"
     CDS             9405..9515
                     /locus_tag="KKO15_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBD6361163.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="KKO15_002405"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="KKO15_002405"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBD6361164.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="KKO15_002406"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="KKO15_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBD6361165.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="KKO15_002407"
     CDS             12397..13029
                     /locus_tag="KKO15_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBD6361166.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="KKO15_002408"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="KKO15_002408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBD6361167.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEEFTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="KKO15_002409"
     CDS             16561..17493
                     /locus_tag="KKO15_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD6361168.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="KKO15_002410"
     CDS             complement(17534..18964)
                     /locus_tag="KKO15_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD6361169.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="KKO15_002411"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="KKO15_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBD6361170.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="KKO15_002412"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="KKO15_002412"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBD6361171.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="KKO15_002413"
     CDS             complement(20825..21094)
                     /locus_tag="KKO15_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBD6361172.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="KKO15_002414"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="KKO15_002414"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBD6361173.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGKWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNHAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="KKO15_002415"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="KKO15_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBD6361174.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5510 a         5588 c         5334 g         5849 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcaccgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agccctctgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacgt ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggggcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgaccggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttcctcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gaactcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcag aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggta agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cacgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAECOD010000061        21975 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.061, whole genome shotgun sequence.
ACCESSION   DAECOD010000061 DAECOD010000000
VERSION     DAECOD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.061"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..580
                     /gene="mglC"
                     /locus_tag="KKO15_002416"
     CDS             <1..580
                     /gene="mglC"
                     /locus_tag="KKO15_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="ABC transporter; functions in galactose transport;
                     part of MglA2C2B transporter complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-methylgalactoside transporter permease"
                     /protein_id="HBD6361175.1"
                     /translation="VYGINSLYYDFVGASPISGFDSGFSTFAQGFVALGSFRLSYITF
                     YALIAVAFVWVLWNKTRFGKNIFAIGGNPEAAKISGVNVGLNLLMIYALSGVFYAFGG
                     MLEAGRIGSATNNLGFMYELDAIAACVVGGVSFSGGVGTVIGVVTGVIIFTVINYGLT
                     YIGVNPYWQYIIKGAIIIFAVALDSLKYARKK"
     gene            complement(616..1828)
                     /gene="preA"
                     /locus_tag="KKO15_002417"
                     /pseudo
     CDS             complement(616..1828)
                     /gene="preA"
                     /locus_tag="KKO15_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(1822..3060)
                     /gene="preT"
                     /locus_tag="KKO15_002418"
     CDS             complement(1822..3060)
                     /gene="preT"
                     /locus_tag="KKO15_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBD6361176.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGGKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(3254..3493)
                     /locus_tag="KKO15_002419"
     CDS             complement(3254..3493)
                     /locus_tag="KKO15_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBD6361177.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(3496..4215)
                     /gene="sanA"
                     /locus_tag="KKO15_002420"
     CDS             complement(3496..4215)
                     /gene="sanA"
                     /locus_tag="KKO15_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBD6361178.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(4365..5249)
                     /gene="cdd"
                     /locus_tag="KKO15_002421"
     CDS             complement(4365..5249)
                     /gene="cdd"
                     /locus_tag="KKO15_002421"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBD6361179.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(5379..6074)
                     /locus_tag="KKO15_002422"
     CDS             complement(5379..6074)
                     /locus_tag="KKO15_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBD6361180.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(6071..6469)
                     /locus_tag="KKO15_002423"
     CDS             complement(6071..6469)
                     /locus_tag="KKO15_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBD6361181.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            6708..7655
                     /gene="dusC"
                     /locus_tag="KKO15_002424"
     CDS             6708..7655
                     /gene="dusC"
                     /locus_tag="KKO15_002424"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBD6361182.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(8026..8109)
                     /gene="yohP"
                     /locus_tag="KKO15_002425"
     CDS             complement(8026..8109)
                     /gene="yohP"
                     /locus_tag="KKO15_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBD6361183.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            8333..9769
                     /gene="mdtQ"
                     /locus_tag="KKO15_002426"
     CDS             8333..9769
                     /gene="mdtQ"
                     /locus_tag="KKO15_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBD6361184.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            9822..10583
                     /locus_tag="KKO15_002427"
                     /pseudo
     CDS             9822..10583
                     /locus_tag="KKO15_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(10700..11278)
                     /locus_tag="KKO15_002428"
     CDS             complement(10700..11278)
                     /locus_tag="KKO15_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6361185.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            11448..12035
                     /locus_tag="KKO15_002429"
     CDS             11448..12035
                     /locus_tag="KKO15_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBD6361186.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            12209..13141
                     /gene="pbpG"
                     /locus_tag="KKO15_002430"
     CDS             12209..13141
                     /gene="pbpG"
                     /locus_tag="KKO15_002430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBD6361187.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13179..14894)
                     /gene="dld"
                     /locus_tag="KKO15_002431"
     CDS             complement(13179..14894)
                     /gene="dld"
                     /locus_tag="KKO15_002431"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD6361188.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            15090..17387
                     /gene="bglX"
                     /locus_tag="KKO15_002432"
     CDS             15090..17387
                     /gene="bglX"
                     /locus_tag="KKO15_002432"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBD6361189.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            17639..18556
                     /gene="osmF"
                     /locus_tag="KKO15_002433"
     CDS             17639..18556
                     /gene="osmF"
                     /locus_tag="KKO15_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBD6361190.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            18563..19720
                     /gene="yehY"
                     /locus_tag="KKO15_002434"
     CDS             18563..19720
                     /gene="yehY"
                     /locus_tag="KKO15_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBD6361191.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            19713..20639
                     /gene="yehX"
                     /locus_tag="KKO15_002435"
     CDS             19713..20639
                     /gene="yehX"
                     /locus_tag="KKO15_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBD6361192.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            20644..21375
                     /gene="yehW"
                     /locus_tag="KKO15_002436"
     CDS             20644..21375
                     /gene="yehW"
                     /locus_tag="KKO15_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBD6361193.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(21356..21463)
                     /locus_tag="KKO15_002437"
     CDS             complement(21356..21463)
                     /locus_tag="KKO15_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBD6361194.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(21523..21975)
                     /locus_tag="KKO15_002438"
                     /pseudo
     CDS             complement(21523..21975)
                     /locus_tag="KKO15_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         5235 a         5574 c         5942 g         5224 t
ORIGIN      
        1 cgtctatggc atcaactcgc tctattacga ctttgtcggg gcgtcgccaa tttctggttt
       61 tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc
      121 ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac
      181 ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa aaatatctgg
      241 tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt
      301 tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg gctttatgta
      361 tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg
      421 gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact atggcctgac
      481 gtatatcggc gtaaacccat actggcagta catcatcaaa ggggcgatta ttatcttcgc
      541 cgtagcgctg gattcactga aatacgcgcg taagaagtga tgtgattgcg gatattgccg
      601 gatgcgtctc tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac
      661 ttccccgagc tcaatacaac ccaccgggca gacgtgacca caaagcagac aacccacaca
      721 tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc
      781 gccgtcgtaa caggaaatat aacagcgtcc acagccaaca catttgtcaa gattgatacg
      841 gggatagaca atataactgc ggtctaaatc ttccgccggg acaatattgt tattcgccag
      901 acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg agataatgcg ataacccgct
      961 cgccatatct tctacatgcg atagccgtac tgcataatgc cggtggtgac ctgtaacgtt
     1021 gctgcgccga gcaataaaaa ctccgccgca tcttcctagg tttcaatgcc gccgataccg
     1081 ctgattggga aatcgcgcag ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg
     1141 atcggtttta ccgctttacc ggaatatccg gaaatactcg atttaccgtt aacgatcggc
     1201 ataccgattt tctgattaag atcgatattg gtgatggatt taacggtgtt aattgccgca
     1261 atgccatctg cgccgccgcg cttcgccgcc agcgccactt cgcacatatc accgatattc
     1321 ggcgtcatct tcgccagcat tggcagcgtg gaaccccgtt tcactgcccg acaatatttt
     1381 tctaccagct ccgggctttg cccgacatcg ctacccatcg catgagaagt catttgcgga
     1441 caggagaagt tacactcgat catatccgcg ccagctgctc ccattgctgc tcattttccc
     1501 ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg
     1561 tcagattctc ttccaacgga tgttcagcaa tctgctccat atttttgaag ccgataaaac
     1621 cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc
     1681 cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta ggctttggca cacatctcat
     1741 agcagttgcc taccggcgaa gaagagaggc agaacgggtt gggaaagttc acgccgcaaa
     1801 aagtaatcga aagatctttc gttaacatga gcaagctccc tctaaatagt gatgaatcgc
     1861 ctcggcggct tctttcccgg ttttcacggc atagaccacg gttttgccac cctcaacaat
     1921 atcgccagca gcaaagactt gcggatcgcg ggtctgataa ttttgtgttt tgatggtgtt
     1981 acgctgctgc tctaactccg caaaggcatc cagtctggca tgctgaccga cggcgagaat
     2041 aattttatct gccgccatcg tcagttcgcc tgataaccgt acatgcttaa acgtgacttt
     2101 attgccttcc acggctactg gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc
     2161 gctggcaaac tctttttcgc ttgccggaaa ctcatctaac tcttcacgtg ctacgcaagt
     2221 taccgcctga cagccgagaa ctttcagcgt gctggctacg tccatcgcga catcaccgcc
     2281 gccgataatt aatgcgcttt gtggaatgct gatatcgcct tgcgcctgtc gtacacgttg
     2341 caggaagtcg acggcaatct caacgtcgct atgctcaaac agcggtagcc cggaaccgct
     2401 tgataacccc acggtgacca gtaccgcgcg gttttccgct ttcagttgct ccagggtgag
     2461 tgttttaccg atttcgttat tgcacttaat ggtcacgccc attttttcaa tacgggcgat
     2521 ctctgcatcc agcactgatt gcggtaaccg gaattgcgga ataccgttac gcaaccagcc
     2581 accggggtgc gcttctttct cataaatcgt gacgtcataa ccctggtttg tcagtgtcac
     2641 actggcctgc aatccggcag gacctgcgcc aataatcgcg actttgccga gcgttttagt
     2701 accgggctga taaatttcca tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa
     2761 gcggccgata tcaatgggag catcaacacc ggcacgggta caaccgcttt ggcataattt
     2821 ctccgtcggg cacactctgg cacaaacggc accgagggca ttattttcgc gaattgtctc
     2881 ggcagcgcct ttaaaattac gaaagtagat tgagcgaata aatttccccg gatcggtctg
     2941 tgccgggcaa gcctgactac agggagcgtc gtgacataat aaacagcgag aggcttcttt
     3001 aatcgccagt aacggcgtaa aagccggagt gagttcatcc agataatttt gttgcggcat
     3061 gtgatcttcc ttaatgctat gagtaattat ggcgaacgct aaataaatat tgcgtgctga
     3121 tattaaggct atgcgcgtgg ggaaatttaa gctgtgaaag gattcacaaa tagctgcggt
     3181 tcagaatgtt atatccgcag cttattgagt gaagtaataa ttattctcaa taattaacag
     3241 aaaagagaaa atattaacga tagaaaataa gataaacaat catgccgata gataaaattc
     3301 cgaagccaat atatgccacc atataagcga agaagagtcc gggatttttt gctcgccaga
     3361 catacaccgg ttccggtgca tttttaaccc gtttatcaat cgcgcctgcg cgccagcctt
     3421 tccatgcttc gcggaaacaa aatatcggga taaggaaaaa aacggcaacg acaaaaaact
     3481 gctgtacatc cataactact ttccttgttt cttttgtaat tcaagtaact gttcgggtgt
     3541 aacggcggga tacccctgcg catcttccgg cacctggtgc atagccggaa tagggaccag
     3601 cggccctaaa aaacgcggtt cacgtttaaa aatataaagg tcagccagcg caccgaaacg
     3661 ggcggcaaat tcgcgaatac gtactgacag catatctttc ggtgacggta cggcataaca
     3721 ctgagcctga atccccatat gcagcgcaat aaataatgct cgctcacagt ggaaacgttg
     3781 ggtgataata atgaaatcat tagtatcgaa aactttgcgt gtacgcacga tggaatccag
     3841 cgtacgaaag cctgcgtaat cgagaacaat atctgatggg tcgacaccag cagcgattaa
     3901 atctttgcgc atggtcatcg gctcgttata actttgcaat gcgttatcgc cgctcagtaa
     3961 tagataattt accttaccgc tgttataggc attaatcgct ccttgaatgc ggtagcgata
     4021 atactgatta ataacgccgg tacgataata ttttgctgtg ccgagcacca cgccgacctg
     4081 gcggtagggg agatcctgca attcgtcgta gatataaggc gcggttttcc agctcatcca
     4141 gcgatcgagg ccgagcacag tcaacagcag caagccgatc aggactaaca ggctgaggaa
     4201 cacgcgcttt aacatgcagg agactcgagc taaggaaaat ttccatcagg ctactttacc
     4261 cgctggtttt gcgcaagaaa cagcaaactg tttgccggga atttcgacac caggagaggg
     4321 ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc aggcttaagc gagaagcacg
     4381 cggtcgatac tgtgacagcc gagagctttc agcgttgcgg aggtggcatc ccactgaatc
     4441 aacggcgcat cggctttttc tgccagaacc gcgcgctgga tatccgggta atcataaccc
     4501 ttgagattca acagaattaa cgccccctgc aacggcggca gagtcgggtt gaatgcggcg
     4561 ttttcagcgt agctgccgct gaaaatacga ccgtctttac attccagcgc gacaccgctt
     4621 ggtgacttac tgtaaggcat gtgcgaacgg tttgccgctg caatcgccgc ctgagaaagc
     4681 gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca tcagcagcgt tttaatctcc
     4741 agatctttcg gcccaaaggc atccggcaga tagtcacgca gcgcgtgtgc ctcgcggccc
     4801 ggcaaatgaa tacgcagatc cagaccgctg ttcagttcat tcataaactg acggcagtga
     4861 ccacaaggcg tatagttaac ggtgatggct gcaagcgctt tttcaccact caaccaagcg
     4921 tggctgattg cgctttgttc ggcatgaacg gtttgctgca ttgttgcacc aataaattcc
     4981 atattggcac caaaatacca ggttccgctc acaccgcgcg caattgcgcc aacattaaaa
     5041 ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa gtagtgcgaa tgccagcgcg
     5101 tcttcgtcca gtcccgttgc gctcttcagc gatgagactt gctccccggt caacaaagcg
     5161 gggaagtact tgtctgccag aataggttcc agtgcagatt gcaagttatc cgcaagttgg
     5221 gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc ataatcatgt aatgggataa
     5281 cagtttacga acccgttatg gctttatatg tgatctgaat ctcattaatt atgcaacacg
     5341 tgaaacttac atcaaaaatg cgcgacgtat cgcaaatttt agcccattac tgccagaata
     5401 atcgggaaaa ggaacggtgc gatcagcgaa gtaattatcc cgcataacac cagcgctagc
     5461 gaactaaatg caccttcctg ataatccagc tcggcgcagc gcgccgtacc gagggcgtgc
     5521 gaggcagtcc ccattgccag accgcgcgca gctttggtac gaatacgcat cgcattaagc
     5581 aatgtatggc caaataccgc gccgaggatg ccgacgaaaa tcacgcaaac tgcgctgatt
     5641 gccggaatac cgccaatact gccgccaacc gccattgcaa taggcgtggt gactgatttc
     5701 ggcaggattg acgcggcgat ttccggtgaa gcgcccatca acaatgccac ggaagtcccc
     5761 gtcaccatcg caaccacgct gccgataaaa cagatggtga tgatcgattt ccagcgcgcg
     5821 cggatctggt gtagctgctc atataaagga taggccagcg cgacgaccgc cggttgcagc
     5881 agatcgttta gcacttcgct acctttaaag taactgtcgt aagagatgcc ggtcagcatt
     5941 aaaaaaggaa tgatcaccac cattgctacc agcaacgggt taagcagggg aaacttatac
     6001 cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg tcagcggtaa tgaccaccag
     6061 atattcgcca tcattcttct gatcctttct gacctacgac tttacgttca ccgtgtacca
     6121 gttgtgaact ccagctcacc accagaaaaa ccaccagcgt actgacagca caggaaacca
     6181 ctaccgggcc aaactgcgcg cggagcaaat caaaatattg catgacgcct acgccaatcg
     6241 gcacaaacaa tagcgccata tagcgaatca gtacgtagca ccccggattg acccatttcg
     6301 ccggaagaat ttgcaaggcc agcaggacaa acaggatctg catcccgatg atgctgcccg
     6361 gaatggttac cggtagcagg gaagcaatga aaatgcctgc atacaggcag gcataaatca
     6421 ggatgaaagc gcgtaaatat tgccagataa tgttcagtgt cttactcata atcaacaccc
     6481 cagatgagat ggattcatca tacaattaat tcgtaaaacg tgctaccgat cacattaaga
     6541 aatcagcgct tattttttcg ctacccggta ttacgcaatg agcagcgaac tcaggcatac
     6601 atcaagcata gagagggatt gcagtttgca gcaatccctc tttgcagacc tttaatccgc
     6661 tgaccatagg cgctaacata gcgcctcatt tttttgcggg tgatgatatg cgtgtgttac
     6721 tggcaccgat ggagggagtg cttgactctc tggtgcgtga attgctgacc gaagttaacg
     6781 actacgatct gtgcatcacc gagtttgtcc gcgtggtgga tcaactgctg ccggtaaaag
     6841 tctttcatcg catttgccct gagctacaaa acgccagccg gacaccatct ggtacgctgg
     6901 tgcgcgtgca gttgttaggt cagttcccac aatggctggc agagaacgcc gcccgtgcgg
     6961 tggagttagg tttctggggc gtggatctca attgcggctg cccgtcgaaa acggttaacg
     7021 gtagcggcgg cggggcgacg ttactcaaag atcctgaact catctaccag ggggcaaaag
     7081 cgatgcgtga agctgtaccg gcgcatttgc ctgtcagcgt gaaagtgcgt ctgggctggg
     7141 acagcggtga gaagaaattt gaaatcgccg atgcggttca acaggctggc gctacggagc
     7201 tggtggtgca tgggcggacg aaagagcagg gttaccgcgc ggagcatatt gactggcagg
     7261 cgattggcga gattcgccag cggctgaata ttccggtgat tgccaacggt gaaatctggg
     7321 actggcagag cgcgcaacaa tgcatggcga tcagcggctg cgacgcagtg atgattggtc
     7381 gcggggcgct caatattccc aacctgagcc gggtggtaaa atataacgaa ccgcgaatgc
     7441 cgtggccgga ggtggttgct ttgctgcaaa aatatacccg tctggaaaag cagggcgata
     7501 ccgggttata tcacgttgcg cggattaaac agtggttgag ttatttgcgt aaagaatacg
     7561 atgaagcaac ggaattattt cagcatgttc gggtgttgaa taactcccct gatattgcaa
     7621 gggctattca ggcaattgat atcgagaaac tctaaaaacc atagtgatgt ttttgacaat
     7681 gtgttagctc tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat cgccgaggcg
     7741 ttccctgaag gccggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg
     7801 ttacctcgcg cccgacccgg tagccgtaag ggataagccg agggcaccgc gcagcggcga
     7861 ttttgttcgc cagagcccgg gggggcaggg ggcggcggcg attggtcgcc ccctgcgcgc
     7921 tccttgcgcc agtgattata tgaagcaatg cacatgacag gagcgcaaca ggatgagaga
     7981 ctgaaatatt ctcgttattt tcggatctga tgacattaat taattttaaa atatcatctt
     8041 aaatacgcca gtcaccacca gtagcccaat caggaaaata atcaatacag cccagagtat
     8101 aattttcatt ggcgttcctt tatttgtgag tgctgaataa gtataggaaa aaacgaagtc
     8161 ctttgccgtt atccataaac gattaagata aatcggaaga tatcatttac ttagtctata
     8221 tttttctggg tatgtcgttt tattgctact gatgtattgc cacgcgaaac taaaaaatat
     8281 atcgcaacac acggtaatct ccgcgcaccg tttcttacct gattattccc ccatgaatcg
     8341 tgattccttt tatccagcca tcgcctgttt tccgctgtta ctgatgctgg ccgggtgtgc
     8401 gcctatgcat gaaacccgcc aggcgttaag ccagcaaacg cccgctgcac aagttgacac
     8461 cgcattaccc acggcgctga aaaatggttg gccagacagc caatggtggc tggagtatca
     8521 cgataatcag ctcacttcat taattaacaa tgcactacag aacgcaccgg atatgcaggt
     8581 ggctgaacag cgcattcagc tagctgaagc ccaggcgaag gcagttgcca ctcaggatgg
     8641 tccgcagatc gacttttcgg cggatatgga gcggcaaaaa atgtcggcag aagggctaat
     8701 ggggccgttt gctctgaacg atccggccgc aggtacgacc ggtccgtggt acaccaacgg
     8761 aacttttggc ttaacggcgg gctggcatct cgatatctgg ggaaagaatc gggcggaggt
     8821 tactgcccgc ctgggtacgg ttaaagcacg ggcggcggaa cgcgagcaaa cccgccaatt
     8881 gctggctggc agcgtagccc gcctgtactg ggagtggcaa acccaggcgg cgttaaacac
     8941 ggtcttgcag caaatagaaa aagagcagaa caccattatc gcgaccgatc gccagctata
     9001 tcagaacggg attacttctt cagttgacgg tgtggaaacc gatattaatg ccagcaaaac
     9061 ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt attgaggcac ggttaaatgc
     9121 actcacaaat caccagacta agtcattgaa gcttaaaccg gtcgcgttgc cgaaagtggc
     9181 aagccagctt cctgatgaac tggggtactc cttactggcc cggcgggcag atttgcaggc
     9241 ggcgcactgg tacgttgagt catcgttaag cactattgat gcggcaaaag cggcatttta
     9301 tcctgacatc aacctgatgg ccttcctgca acaggatgcg ttgcacttaa gcgatctgtt
     9361 ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg acgctaccta ttttcgatag
     9421 tggtcgtctt aacgccaatc tcgatatcgc aaaagccgaa agcaacttgt ctatcgccag
     9481 ctacaacaaa gcggtggttg aagcagtgaa tgacgtggcg cgggcagcca gtcaggttca
     9541 gacactggcg gagaaaaacc agcatcaggc gcaaattgag cgcgatgcct tgcgtgtggt
     9601 aggtcttgcg caggcccgct ttaacgcggg catcattgct ggttcccgcg tcagcgaagc
     9661 cagaatcccg gcgctgcgtg agcgggccaa tggcctgtta ttgcaagggc agtggctgga
     9721 tgcctccatt cgactcaccg gtgcgctggg cgggggttac aaacgctgat gatatgcgcc
     9781 ttctatactt aacgtttatt cagcgttaag tggagaactc gatggcatag gtcgcgataa
     9841 ttaccgcttc cgattcgggg atcggcaaag agtgcgcgtt attactggcg cagcaggggt
     9901 ttgatattgg cattacctgg cactcagatg aagaaggggc aaaagatacc gcgcgtgagg
     9961 tggttaccca cggcgtacgt gcggagatcg tgcaactgga tctcggcaat ctgccagaag
    10021 gtgcgcaggc gctggagaca ctcatccagc gatttgggcg tattgatgtg ctggtgaata
    10081 atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc ttttgatgag tggcgcaaga
    10141 tttttaccgt tgatgttgat ggtgcatttt tatgctcgca aattgcggct cgtcagatgg
    10201 tgaaataagg gcagggcggt cgcatcatca acattacgtc ggtacatgaa catacgccgc
    10261 tgccggatgc cagcgcctac acagccgcta aacatgcgct cggcgggtta accaaaacga
    10321 tggcgctgga gttggtcagg cataagattt tggtgaacgc agtcgcgcct ggggcgatag
    10381 ccacgccaat gaacagcatg gatgatagcg acgtgaagcc cgacgcggag ccttcgattc
    10441 ctctacggcg ttttggcgca acgcatgaga ttgccagcct ggtggtgtgg ctttgttcgg
    10501 agggcgcaaa ttacacgaca gggcagtcgt tgatagtgga tggcggcttt atgttggcga
    10561 atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg atatttcaat gggttaggcc
    10621 agataagaca ccagcgtcgc atcaggcatg gaacacccgg tgccggatgt ggagtgaatg
    10681 cttatctgtg gcaactgctt tacgcctgat tatccggctt tttcttcccg cgccgtttca
    10741 gccaccagcg cacgcccacc accagaacca cgaccagaat caaccagacc cagtgcttca
    10801 aatgctggtc gagattgtgc aaccacggcg caatcacctg accaccagcg taaccaatag
    10861 tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt cagcggcaga aagattttcg
    10921 gcggcagctg gctggcacca atcagcgtcg ggccaatcac ccgaaagcca tacataaagc
    10981 gcgtaccaat gacaaacaga tacggatggc gttggataag tttctgtgcc cgctcaattt
    11041 tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc aaaccgccgc ccgcacagat
    11101 agagcacctg gtcgccaatc atgccaccaa gcgccacaga aagtaccacc agtgggaact
    11161 ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt cacggtttca ccttccgcca
    11221 ggctaccgat aaccagcgcg gcataaccat attgtgagat gagtgtattg agatccatag
    11281 gccagtttgt ctccctttgg ttgcctgtaa gcatacaccc tgtgaatcat tcacgctgaa
    11341 aaaggacata catcagacgg tgcaaattac atggatgaac aaaaaataat cacaagctgg
    11401 cttatacttg aactgtcagg ttcatgaact cacaacaagg gggcggtatg agccatgtct
    11461 ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat taatcgcgaa aacgaaacga
    11521 tttctcacca ttacacccac cacgttttgc tgatggcggc gattccggtg atttgcgcct
    11581 tcattggcac tacacagatt ggctggaatt ttggcgatgg cactatcctg aagttatcct
    11641 ggtttaccgg actggcgctg gctgtcttgt tttacggcgt tatgctggct ggggttgcgg
    11701 tgatggggcg ggtcatctgg tggatggcgc gcaattaccc gcagcgtccg tctcttgcgc
    11761 actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt tttaagcggt ctggtggcgc
    11821 tttatccact ggtctggttg tgtgcgctgg tcggcacggt ggcgctgttt tacaccggct
    11881 acctgcttta ccttggtatt ccgagcttcc tgaatatcaa caaagaggaa ggccttagct
    11941 tctccagttc gacgctggcg attggcgtgc tggtactgga agtgctgctc accctaaccg
    12001 ttatactatg gggctacggt tatcgtttgt tctagaaagt tctttgacgt tgcattgctg
    12061 gcgtaaatgt cagcaatgca gcatttactc acgattctcc tttgacgatc gcactttttg
    12121 ctcgttatga tggcgtcgct agcctcagta aatccttact ccggcggtgc gcaacccgtg
    12181 cgcgtgaacc actatctgaa tgctcatcat gccgaaattt cgagtttctt tatttagctt
    12241 ggccctgatg ctggctgtgc cttttgcacc gcaggccgtt gcgaaaacgg cagccgctac
    12301 caccgcttca caaccggaaa ttgcctccgg tagcgcgatg attgttgatc tgaataccaa
    12361 caaagtgatc tattcgaacc acccggatct ggtgcgtccg attgcgtcta tcagcaaatt
    12421 aatgaccgcg atggttgtgc tggatgcacg actgccgctg gatgaaaaac taaaagtgga
    12481 tatcagccag acgccggaga tgaaaggggt ctattcgcgc gtacgactga atagcgaaat
    12541 cagccgtaaa gatatgctgt tgctggcgct gatgtcttca gaaaaccgcg cggcggcaag
    12601 ccttgcgcac cattatcccg gtggttacaa agcctttatt aaggcaatga atgcgaaagc
    12661 gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct accggattgt cggtgcataa
    12721 cgtttcaact gcccgtgact taaccaaact gctcattgcc agcaaacaat atccgttgat
    12781 cgggcagtta agtactaccc gggaagatat ggcaaccttc tctaacccga cgtatacgct
    12841 gccgttccgc aatactaatc atctggtgta tcgcgataac tggaatattc agttaaccaa
    12901 aaccggcttt accaatgcgg cgggccattg tctggtgatg cgtacggtta tcaataataa
    12961 accggtggcg ctggtagtga tggacgcgtt tggcaaatat acccattttg ccgatgccag
    13021 ccgcctgcgt acctggattg aaaccggtaa agtgatgcct gtgccggcag cagcgttgag
    13081 ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg cagacggcac agaacgatta
    13141 agtgaattcg gatggcgata ctctgccatc cgtaattttt actccacttc ctgccagttt
    13201 ttccgtttac tggttttacc gatccccgga ttcatgctat tggtcggatc attctcgcga
    13261 tagaacttct gcaacgtctc cggtgcttta tacaaatgac cgacgttatg ctcggcaggg
    13321 tactgcgcgc cgcgctgctg tagcagttcc agcatctgtt cttttaacgc atgcacatcc
    13381 acgccttttt tcactatgta atcctgatgg aagacatagc acataaaatg gccgtaatag
    13441 agcttgtgca ccagctggct gtcgatctcc ggcggtaaat gctcatacca ctcggtgtcg
    13501 ttacgccgca gagcgatatc cagtgccaga atgtcttcga cttcatcgga atgcaccgcc
    13561 tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta aaaacgcttt gctaccttcc
    13621 tccggcgtac agacaaagaa atcgccctcg gcctgtttga aataatccac cagccacgat
    13681 ttggcttcac cgacgccatc gcctgccatt tttaacagca gatgatgctc gtatttatcg
    13741 cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca ggtgaccgaa tttttgcatt
    13801 gcacggtcag taaaatgcgg acggaagaat ttcactttct ccagcatcgc atcggtgcgt
    13861 cccttgagat taaagaagaa cggcatcttg tcggtgccta acttatcaat catcaggaag
    13921 gtgtctttgc cgtatttttc cgcaatatcg tagatatccc ggtgcatata ttccccggca
    13981 accggcagat tttcgaagtt agccagaata tgacggcgga tttcggtcag cacttccggc
    14041 tggttggtgc cgatataaaa cacctgctga tttttttccg cttcgaaggt atcaagacgt
    14101 accgcaaaga cggccagctt cccggcgcaa ccagaagatt caaataaccg atccggatcg
    14161 gcgttataac gtgcgggcgt gtcggcttca atatcacgaa cgcggtggac atagtcataa
    14221 tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt tgatgcgatc gtcatccagc
    14281 ttgctaagga tctgctccgg cgtttcgccc agatcaatcc ccagatggtt caccagcgtc
    14341 agtttgccat cttcatctat acgcgcgaat aacgacattt cggtatacgc cgggccacgt
    14401 tgcaccagcg agccgccgga gttgttacaa ataccgccga tgaccgatgc gcctatacac
    14461 gatgatccaa tcaccgaatg cggttcgcgt cccagcggtt tgagggcttt ttccagcgaa
    14521 tagagcgtgg tgcccggata ggccagcacc tgttcgccct tgccgagaac gtgcagcttg
    14581 tcgagacgca gggtgctgat gatcacgata tcgcgatcat agtcgttacc gtttggcgtc
    14641 gatccttcgg tcaggcctgt attggcagcc tgcatcagaa taattttgtc ggcggtgacg
    14701 caggctttca gcacccgcca caattccagt agtgagccag ggaaaacgac agccagcgcg
    14761 tcgccctgac cagaacggaa gcccttgcga tagcgggccg tttttgcggg atcggtgagc
    14821 aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa aggctttatt atcagttgtt
    14881 gtcatggaag acattttcca ctccttgtgg tggcgaaaaa atatcacaaa ctagaatagc
    14941 gccttgtatg acaattcagc gagtgaatat tttgaaaatt cagacaataa actgagcatt
    15001 ctttttctct ctccatcatg cttatggcaa actacggttt ttgcaccatt ttcgctgcca
    15061 cgtcgggcaa caaaggaaga aaaatccata tgaaatggct atgttcagta ggaatcgcgg
    15121 tgagtctggt cctgcagcca gcactggcgg atgatttatt cggtaaccat ccattaacgc
    15181 ctgaagcgcg ggatgcgttc gtcaccgatc tgctcaagaa aatgacggtt gatgagaaaa
    15241 ttggtcagct gcgcttaatc agcgtaggcc cggataatcc gaaagaggcg atccgcgaga
    15301 tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt aacccgtcag gatatccgcg
    15361 ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat tcctcttttc tttgcttacg
    15421 acgtgctgca cggtcagcgc acggtgttcc cgattagcct cggtctggcc tcgtctttta
    15481 acctcgatgc ggtgaaaacg gtcgggcgtg tctctgctta tgaagcggca gatgatggcc
    15541 tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga tccgcgctgg ggacgtgctt
    15601 ccgaaggttt tggcgaagat acgtatctca cctcaataat gggtaaaacc atggtggaag
    15661 cgatgcaggg taaaagcccg gcagatcgtt actcggtgat gaccagcgtc aaacactttg
    15721 ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac cgtcgatatg agtccgcagc
    15781 gcctgtttaa tgattatatg ccgccgtaca aagcggggct ggacgcaggc agcggcgcgg
    15841 tgatggtggc gctgaactcg ctgaacggca cgccagccac ctccgattcc tggctgctga
    15901 aagatgttct gcgcgaccag tggggcttta aaggcatcac cgtttccgat cacggtgcaa
    15961 tcaaagagct gattaaacat ggcacggcgg cagacccgga agatgcggtg cgcgtggcgc
    16021 tgaaatccgg aatcaacatg agtatgagcg acgagtacta ctcgaagtat ctgcctgggt
    16081 tgattaaatc cggcaaagtg accatggcag agctggacga tgctgcccgc catgtactga
    16141 acgttaaata tgatatgggg ttgtttaacg acccatacag ccatttgggg ccgaaagagt
    16201 ctgacccggt ggataccaat gccgaaagcc gcctgcaccg taaagaagcg cgtgaagtgg
    16261 cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac gttaccgctg aaaaaatcgg
    16321 ccaccattgc ggtggttggg ccactggcgg acagtaaacg tgacgtgatg ggcagctggt
    16381 ccgcagccgg tgttgccgat caatccgtga ccgtactgac cgggattaaa aattccgtcg
    16441 gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt taccagtgac aaaggcatta
    16501 tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga tccgcgttcg ccgcaagaga
    16561 tgattgatga agcggtgcag acggcgaaac aatctgatgt ggtggtggct gtagtcggtg
    16621 aagcacaggg gatggcgcac gaagcctcca gccggaccga tatcactatt ccgcaaggcc
    16681 aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc gctggtgctg gtgctgatga
    16741 acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc tgatgcgatt ctggaaacct
    16801 ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt attgtttggc gattacaacc
    16861 cgtccggcaa gctgccaatt tccttcccgc gttctgtcgg gcagatcccg gtgtactaca
    16921 gccatctgaa caccggtcgt ccgtataatg ccgacaagcc gaacaaatac acttcgcgtt
    16981 attttgatga agctaacgga gcgctttatc cgttcggcta tggtctgagc tataccactt
    17041 tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa gcgtgacggc aaagtgacgg
    17101 ccagcgtgca ggtgacgaac accggtaagc gcgagggggc cacggtagtg cagatgtact
    17161 tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca gctgaaaggc tttgagaaaa
    17221 tcaccctgaa accgggcgaa actcagactg tcagcttccc gatcgatatt gaggcgctga
    17281 agttctggaa tcaacagatg aaatatgacg ccgagcctgg caagttcaat gtatttatcg
    17341 gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt gctgtaatgc tttagtaagg
    17401 gcgcgacgtt tgcgcccttt gcctgatgtt catatcttaa cttgctgaat tttcgccctt
    17461 tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa acgccggacc gaatatgcct
    17521 cttaagtatt tatctgattc tgcaactgaa tccttccgct caggctaact ctgtcattat
    17581 caactatgct tttctcttaa ttcgctgaaa acaacagtaa aagaatgagg aaagcatcat
    17641 gccactctta aagctctggg caggttcact ggttatgttg gcagccgtga gcctgccgct
    17701 acaggcggct tcccccgtta aagtcggttc aaaaatcgat accgaaggcg cgctgctcgg
    17761 caacatcatc ttgcaggtac tcgaaagcca cggcgtaccg acggtgaata aagtgcaact
    17821 cggaacgact cctgtggggc gcggggcgat tacttccggt gaactggata tctatccgga
    17881 atataccggc aatggcgctt tcttctttaa agatgaaaac gatgcagcgt ggaaaaatgc
    17941 cgggcagggc tacgagaaag tcaaaaagct cgatgcggag cacaacaagt taatctggct
    18001 gacgcccgcg cctgcaaata acacctggac tatcgccgtg cgtcaggatg tggcagagaa
    18061 aaacaaactc acttcgcttg ccgacctgag tcgttatctg caagagggcg gcaccttcaa
    18121 actggcagcc tcggcagagt ttatcgaacg cgccgatgcg ttacccacgt ttgaaaaagc
    18181 ctatggcttt aagctcggtc aggatcagtt gctgtcactg gctggtggcg acacggcggt
    18241 gacgattaaa gccgctgccc agcaaacctc tggcgttaat gccgcaatgg cttacggcac
    18301 tgacggtccg gtcgcggcgc tggggctgca aaccttaagc gatccgcaag gcgtgcaacc
    18361 tatctacgcg cctgcaccag tggtgcgtga atcggtgttg aaggagtatc cgcaaatggc
    18421 acagtggcta cagccagtct tcgccagcct cgatgcaaaa acattgcagc aactgaatgc
    18481 cagcattgca gtggaaggac tggatgccaa aaaagtggct gccgactacc tgaaacaaaa
    18541 agggtgggcg aagtaatttc ccgtgactta tctacgtatt aatcctgttc tggcgctgct
    18601 gttgttgctg acggcaatcg cagcggcgct gccgtttatc agttacgcgc ctaatcgttt
    18661 agtctcgggt gaagggcttc atctctggca actgtggccg caaacgatct ggatgctggt
    18721 gggcgttggt tgcgcctggc tgacagcctg ttttgtcccc gctaaaaaag gcagcatttt
    18781 tgcactcatt ctggcgcaat tcgtcttcgt attgttggtg tggggagctg gaaaggcggc
    18841 gacccaactg gcgcaaaatg gcagtgcgct ggcgcgtacc agcctcggca gaggtttctg
    18901 gctggctgcg gcgctggcat tgctggcctg tagcgatgcc atccgccgaa tctccacgca
    18961 tccgctgtgg cgctggttgt tgcatatgca gattgccatt attccgctgt ggttgctgta
    19021 ctccggcacg cttaacgatc tctcactaat gaaagaatac gccaaccgtc aggatgtgtt
    19081 tgacgacgcg ctggcacaac atctgacgtt gctgtttggt gcggtgctgc ctgcgttagt
    19141 gattggtgtg ccgttgggca tctggtgcta cttttccact gcgcggcagg gggcaatttt
    19201 ttctctgctc aatgtcattc agaccgtgcc ttcggtggcg ctctttggcc tgttgattgc
    19261 gccgcttgcc gcgctggtta cggcctttcc gtggctgggg aagctcggca tagcaggaac
    19321 cggaatgaca cccgcactga ttgcgctggt gctctatgcc ttgctgccgc tggtgcgagg
    19381 cgtggtagtc ggtttgaacc agatcccgcg cgatgtgctg gagagcgcca gagcgatggg
    19441 gatgagcggg gcgcagcgtt tcctgcatgt tcagttaccg ctggcgttac tggtatttct
    19501 gcgcagcctg cgggtggtga tggtgcaaac tgtaggtatg gcggtgattg cggcgttaat
    19561 cggcgcaggc ggttttggtg cgctggtttt ccaggggctg ctaagcagcg ccattgattt
    19621 agtgttgctg ggggtgatcc cggtaattgt tctggcggtg cttaccgacg cgctgttcga
    19681 tttgcttatc gcactgctga aggtgaaacg taatgattga atttagccat gtcagcaaac
    19741 tgttcggcgc acaaaaagcc gttaacgatc tcaatctcaa ttttcaggaa gggagttttt
    19801 cggtgctgat tggcacatct ggctccggca aatccaccac cctgaaaatg attaaccgcc
    19861 tggtggagca tgacagcgga gtgatccgct ttgccggaga agaaattcgc tcgctgccag
    19921 tactggagtt gcgccgccgg atgggctatg ccattcaatc tattggcctg ttcccccact
    19981 ggagcgtggc gcaaaacatc gctaccgtgc cgcaattaca aaaatggtcg cgggcgcgga
    20041 ttgacgatcg tatcgacgaa ttaatggcgc tactggggct ggagtcaaat ttgcgtgagc
    20101 gttatccgca tcagctttcc ggtggtcagc agcaacgtgt gggagtggcg cgtgcactgg
    20161 ctgccgatcc gcaagtctta ctgatggatg aaccttttgg cgcactggac ccggtaacgc
    20221 gcggcgcgtt gcaacaagag atgacgcgca ttcaccgttt gctggggcgc actattgtgc
    20281 tggtcactca tgatattgat gaggcgctac ggctggcaga acatctggta ttgatggatc
    20341 acggtgaagt ggtgcagcag gggaatccgc tgacgatgct gactcgtccg gcgaatgatt
    20401 ttgtccgcca gttttttgga cgtagtgaac tgggggtgca cctgctttcg ttacgtagcg
    20461 tggcggatta cgtgcgtcgc gaagaacggg cagaaggtga ggcactggca gaagagatga
    20521 cgctacgcga tgcgctctcc ctgtttgtcg cgcggggatg cgaggtgctg ccggtggtga
    20581 acacgcaggg ccagccttgc ggcacgctgc attttcagga tctgctggtg gaggcgtaag
    20641 cgtatgaaga tgttgcgcga tccgctgttc tggctcattg ctctttttgt ggcgctgatt
    20701 ttctggctgc cttacagcca gccgctgttt gctgccttgt tcccacaact gccacgaccc
    20761 gtttatcagc aagaaagttt tgcagctctg gcactggctc atttctggct ggtgggaatt
    20821 tcgagtttgt ttgcggtgat cattggcaca ggtgccggaa ttgctgttac tcgcccgtgg
    20881 ggcgcggaat ttcgcccact ggtggaaact attgccgccg ttggacagac ttttccgcct
    20941 gtcgcagtgc tggcgattgc cgttccggtg atcggctttg gtctgcaacc agcgattatc
    21001 gccttgatcc tttacggtgt gctgcccatc ctgcaggtga cacttgccgg gctgggagcg
    21061 attgatccca gcgtgacaga agttgcgaaa ggtatgggaa tgagtcgagg tcagcgactg
    21121 cgtaaggtcg agctaccgct ggcggctccg gtgattctgg cgggcgtgcg aacttcggtg
    21181 attatcaaca ttggtacggc gacgatcgcc tcaacggtag gggccagtac gctgggtacg
    21241 cccatcatca tcgggcttag cggatttaat accgcgtatg tgatccaggg ggcgttactg
    21301 gtggcactgg cggcgatcat cgcagaccgc ctgtttgaaa ggctggtgca ggcgcttagc
    21361 cagcacgcaa aataaaggta taacctgcga gcatgacgcc accaattccg cctaacgcca
    21421 taaacaggaa cagggcgatg accccaattt tagctatgcg cataatgcac tccttatgtt
    21481 aacgaaagga ttgtacagta aagcgcattt gttaacgaat cattaaatac cgagtgggaa
    21541 aatatcatgg ccttgttctt gccaactggt gagttgctgc tgttgggcgg aggttcgatt
    21601 ttcaccgcac cacaccagca atgtacggcc ttcgaatagt tcagggcgta gttgattgag
    21661 cgagtgggcg agcgaggaca tcaatgcgcc atccttgttg actggcaatc cagccctcca
    21721 gccacagacg ggtggtatcc tgaatattcc agccaaccac cagcgcatct ttaccctgtt
    21781 ttttacgtgc cgaagccaga caaatggcga tgtagttgat cagtacgccg tcgaggatcg
    21841 ccagcagcgc ctggagagtc ggttgttggc actgaagccg tcggcgcaga ggaataaaca
    21901 gatgtgtggt gagtgtctgg gcggggtaat cctgaccgcg ctctttgatc cacgttcgca
    21961 ggctatgtag attgc
//
LOCUS       DAECOD010000062        21770 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.062, whole genome shotgun sequence.
ACCESSION   DAECOD010000062 DAECOD010000000
VERSION     DAECOD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.062"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..158)
                     /locus_tag="KKO15_002439"
     CDS             complement(<1..158)
                     /locus_tag="KKO15_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBD6361195.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLN"
     gene            complement(151..1041)
                     /locus_tag="KKO15_002440"
     CDS             complement(151..1041)
                     /locus_tag="KKO15_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBD6361196.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1071..2327)
                     /gene="wzx"
                     /locus_tag="KKO15_002441"
     CDS             complement(1071..2327)
                     /gene="wzx"
                     /locus_tag="KKO15_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBD6361197.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2343..2888)
                     /gene="rfbC"
                     /locus_tag="KKO15_002442"
     CDS             complement(2343..2888)
                     /gene="rfbC"
                     /locus_tag="KKO15_002442"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBD6361198.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2893..3771)
                     /gene="rfbA"
                     /locus_tag="KKO15_002443"
     CDS             complement(2893..3771)
                     /gene="rfbA"
                     /locus_tag="KKO15_002443"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD6361199.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3830..4729)
                     /gene="rfbD"
                     /locus_tag="KKO15_002444"
     CDS             complement(3830..4729)
                     /gene="rfbD"
                     /locus_tag="KKO15_002444"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBD6361200.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4729..5814)
                     /gene="rfbB"
                     /locus_tag="KKO15_002445"
     CDS             complement(4729..5814)
                     /gene="rfbB"
                     /locus_tag="KKO15_002445"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD6361201.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6187..7080)
                     /gene="galF"
                     /locus_tag="KKO15_002446"
     CDS             complement(6187..7080)
                     /gene="galF"
                     /locus_tag="KKO15_002446"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBD6361202.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7255..8649)
                     /gene="wcaM"
                     /locus_tag="KKO15_002447"
     CDS             complement(7255..8649)
                     /gene="wcaM"
                     /locus_tag="KKO15_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBD6361203.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8660..9880)
                     /gene="wcaL"
                     /locus_tag="KKO15_002448"
     CDS             complement(8660..9880)
                     /gene="wcaL"
                     /locus_tag="KKO15_002448"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBD6361204.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9877..11157)
                     /gene="wcaK"
                     /locus_tag="KKO15_002449"
     CDS             complement(9877..11157)
                     /gene="wcaK"
                     /locus_tag="KKO15_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBD6361205.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11337..12815)
                     /gene="wzxC"
                     /locus_tag="KKO15_002450"
     CDS             complement(11337..12815)
                     /gene="wzxC"
                     /locus_tag="KKO15_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBD6361206.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12817..14211)
                     /gene="wcaJ"
                     /locus_tag="KKO15_002451"
     CDS             complement(12817..14211)
                     /gene="wcaJ"
                     /locus_tag="KKO15_002451"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBD6361207.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14266..15636)
                     /gene="cpsG"
                     /locus_tag="KKO15_002452"
                     /gene_synonym="manB"
     CDS             complement(14266..15636)
                     /gene="cpsG"
                     /locus_tag="KKO15_002452"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBD6361208.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15829..17265)
                     /gene="cpsB"
                     /locus_tag="KKO15_002453"
     CDS             complement(15829..17265)
                     /gene="cpsB"
                     /locus_tag="KKO15_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBD6361209.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17268..18491)
                     /gene="wcaI"
                     /locus_tag="KKO15_002454"
     CDS             complement(17268..18491)
                     /gene="wcaI"
                     /locus_tag="KKO15_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBD6361210.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLVTAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18488..18967)
                     /gene="gmm"
                     /locus_tag="KKO15_002455"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18488..18967)
                     /gene="gmm"
                     /locus_tag="KKO15_002455"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBD6361211.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18967..19935)
                     /gene="fcl"
                     /locus_tag="KKO15_002456"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(18967..19935)
                     /gene="fcl"
                     /locus_tag="KKO15_002456"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(19938..21059)
                     /gene="gmd"
                     /locus_tag="KKO15_002457"
     CDS             complement(19938..21059)
                     /gene="gmd"
                     /locus_tag="KKO15_002457"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBD6361212.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(21085..21633)
                     /gene="wcaF"
                     /locus_tag="KKO15_002458"
     CDS             complement(21085..21633)
                     /gene="wcaF"
                     /locus_tag="KKO15_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBD6361213.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21649..>21770)
                     /locus_tag="KKO15_002459"
     CDS             complement(21649..>21770)
                     /locus_tag="KKO15_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBD6361214.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5435 a         5737 c         5016 g         5582 t
ORIGIN      
        1 atatttaatg tattatcagt tgataaatca tctgatataa aaattgagat gtctacatct
       61 ttttgttcaa taatactttt taattgttcc tcaatccagc attcgccatt atacgtagcc
      121 ataagtacag ctactctttt ttgatcagaa ctatgcatta taacgaccgc ccccagtaat
      181 tcctcttatt ccatccagcg tagccaaaat atgttttttc atatattcaa ttttattttt
      241 ttgagttgcg ataattaaag tcgtatgaat taaagaaaaa acaacttccc taatagagag
      301 taaaagaggc acatgatttt ttctaagcaa aagaaatgca tttcttactc gataataacg
      361 acgtactggc gagtgaattg gtatgttaat cccacagatt tttactgact tatccccaat
      421 agcatgaacc atttctatat caggaatgat atgaactaat atgccatagt ttaagcatct
      481 aagacaccat tctgtatcga cataatcaat aaaaagttcc tcatccatca ttccaacaat
      541 ctcaaaaact tcttttgaaa ccatggttcc ggatgagatt gttactgaac ttttaaacgg
      601 tgtctgtcct tcagtgacat gaattttctc tcgaagacca ttttttttga tattacaaat
      661 aggataataa tgtgacttat ctctatcaat aaaaactggg ccagctattt ttgcattttc
      721 attatccata tagcaggaaa gttttttgat aaactcattg cgaatacggc tatcttgatc
      781 aaaaaaaatt atcttctctg cccctttttc aagagaatat cgaatcccaa tattttgtgc
      841 ttttgctata ccttcattcc taccaagaca aattaaatga actttttcaa tgtctgctaa
      901 tttatctttc aaggtgaaat tagtcctatt atcgactact acaacgtaac aattttgctc
      961 ctttagttca gtgatcagtg cattcagatt tttaagttcg ggattatatg tcacaatgac
     1021 agcgtaaata ttactattca ttatccaagt gactcagtaa ttggttaatt ttagttttca
     1081 tatacagaac acattctagt ataaagccat tgtaataaca gagcatctaa aattacacgt
     1141 atgctccagg atattgcggc acccagtaac ccatattttt cgaccataaa atacaaaagg
     1201 gctaaataag gaaccaattc agcacaatga atcaaagcag taattttaga ttttcctaat
     1261 gcctggattg cagagaatgg tatcattgcc agcgcattga agaaaaaacc gatcaataac
     1321 acatttaata tattaaagga attaatttga taattctcac caaaccatat tttcaataca
     1381 agccctgaat ataacaagcc tatgattatc accggcaaac agattagaaa cattaaaagc
     1441 aatgatttat ttacattacg tttaaattct ttaaagtctt tgatgttact taaccttgga
     1501 aacactgccc tcccgattgc tgcaggtatt attcctaatt ttagaataac ctccgctggt
     1561 gcagaataaa atgcaacttt atctgccccc ataatatttg acactataaa tcgatcaaaa
     1621 tacaccatga ccggacttat gatattactt actgtcatcc aaccgccaaa gaaaaagagt
     1681 cgtttaaagg ttttataaca aaaatgaacc cccgaaattt taatatcatt cctgacataa
     1741 taggcagaga ctaaaatcga aataactctt gcaaatatta aaccagcaac cgctgccgac
     1801 aatgtagcac tataaaaaac aaatatggcc ggaattcccg cgatgcaaga gcttgatata
     1861 gatttttgaa tatttacaat gccaaatttt tcatccccct caagaatggc tgaccataat
     1921 tgatttagaa taaataatgg aatgcaaata gccaacagtt taaatgctag ttgtatatca
     1981 ctatgttcaa caccagaaat ttttagataa ttaacaattc catcagagaa aatcaataat
     2041 aaaaaagcac caaagcatga aaatagcacc aaaaaagatg tacttgttga aattaccttg
     2101 gttctttcat gatgattatc acgatgaatt gcaatttctc taataacaga gcgagtcagg
     2161 cctacatcaa aaattccagc atacccaaca agtgcaattg caattgtata aataccgaaa
     2221 ccttcaggcc ccaaacctcg agcgagaaat cctagcgagg ggatagcaat taaagttggt
     2281 attgcatagc caaaaaggtt ccagacacta ttttttatta tgctcattat tacatttctt
     2341 tatcatataa aataatcagc atctacaaat ttttgaccat taatatcttt atcagaaagc
     2401 aatggatttt gaaccggcca ttctatattt atacttttgt cgctccataa aatcccacgc
     2461 tcttgcattt ttgaatagaa attatttgtt ttgtagacaa attctgcttc atcactaaga
     2521 acgcaaaacc catgagcgaa tccttccggt atccataatt gacgcttatt ctccgccgac
     2581 aaatttacac caacccactt gccaaatgtt ggtgatgatt tccgaatatc aacagctacg
     2641 tcaaaaactt caccgacaac acagcgtacc agttttcctt gagcataagg ttccagctga
     2701 tagtgcagtc cgcgtaacac acccttagtt gatttagaat ggttatcctg aacaaactca
     2761 acctttcgtc ctacagcctc ttcgaaaact ttctgattga agctttccat aaaaaagccc
     2821 cgctcatcac caaatacttt tggttcaaaa attagcacat caggaatttc agttttaatt
     2881 acgttcattt tattaataac ctttaatcat tttcagcaga tactgaccat aagcattttt
     2941 cttcagcggc tcggctaata ccttcacttg ctcagcatca ataaagcctt tacggtaagc
     3001 aatttcttcc gggcaggaaa ctttcagccc ctgacgctct tcaattgttg caatgaagtt
     3061 gcttgcttca ataagacttt gatgtgtccc cgtgtccagc catgcataac cacgccccat
     3121 catagcgaca gacaaacgtc cttggtccat ataaatacgg ttaatatcgg taatttccag
     3181 ttcgccacgt gcagatggtt taaggttttt cgccatttct acaacgtcat tgtcatagaa
     3241 ataaagccca gtaaccgcat agttactttt tggttccagc ggtttttctt ccagactaat
     3301 tgccgtaccg ttatcatcaa attccaccac accgtagcgc tcaggatcat ttacgtgata
     3361 ggcaaaaacc gttgcgccac tctctctgtt aacagcggta tccattaatt tcggcaggtc
     3421 gtgaccatag aagatattat caccaagtac cagcgcacaa tcatcaccac ctataaattc
     3481 ttcaccaatg ataaatgctt gcgccagacc atccggactc ggttgtactt tatattgcag
     3541 attaagcccc cactggcttc catcaccaag caattgttga aaacgtggtg tatcctgcgg
     3601 cgtactgata atcagaatat ctcgaatacc cgccaacata agcgttgaaa gcggataata
     3661 aatcatcggt ttatcataaa tcggtagcag ttgtttgctc acagccattg tcacaggata
     3721 aagacgagtg ccggaaccac cagccagaat aataccttta cgcgttttca tttcaccatt
     3781 ccttttaatt catctcgctc tagcatcatg agcgagatgc aataatttgt taaattgccg
     3841 tggtcgtaaa taattcgttg agcatacgtt tcacgcccac ctgccagtca ggcaagacaa
     3901 gcgcaaagtt ctgctgaaat ttttcggtat tgaggcgaga attatgggga cgacgtgctg
     3961 gtgtgggata ggccgttgtc ggcacggcat taagtttgtt aagagcaagg ttgatccccg
     4021 ctctgcgcgc ttcttcaaat accagagcgg cataatcgtg ccaggttgtt gtaccaccag
     4081 caaccagatg gtacaaacca gcaacttctg gtttatttgc ggccacgcga atggcatgag
     4141 cggtgcaatc agccagtaat tcagcccccg ttggtgcgcc gaattgatcg tttattacag
     4201 ccaactcttc gcgttctttt gccaggcgca acatcgtttt ggcaaagtta tttcctttac
     4261 cagcgtatac ccagctggta cggaaaataa gatgctttgc gcaatgttct tgtaacgctt
     4321 tttccccagc tagcttggtt tcaccgtaga catttagcgg tgctgttgca tccgtttcca
     4381 gccatggcgt gtcaccgttg cctgggaaaa cataatcagt ggagtagtgt ataacccagg
     4441 ctccgacttc attagctgct tttgcaatcg cttcgacgca tgtcgcgtta agtaattgcg
     4501 cgaaattcgg ttctgattcc gctttgtcta ctgcagtgtg agcagcagca ttaacaataa
     4561 catcggggcg aatttttttg acggtttcag ccacaccttc ggggttgctg aaatctccac
     4621 aataatcagt ggagtgaaca tcgagcgcaa tcaagttacc cagaggagcc agagcacgct
     4681 gcagttccca acccacctgc cctgttttgc cgaaaagcag gatattcatt actgacggcc
     4741 ctcatagttt tgttcaatcc acgactgata agcaccgctt ttcacattct caacccaatt
     4801 tgtattagcc agataccatt ccaccgtttt acgaatcccg ctctcaaacg tttcttgcgg
     4861 tttccagccc aattcgcggc taattttatc ggcatcaatg gcgtaacggc ggtcatgccc
     4921 cgggcgatcg gcaacataag taatttgctc acgataagat ttctctttcg gtacaatctc
     4981 atccaacaaa tcacaaatag tgagcactac gtcgatgttt ttcttttcgt tatgtccacc
     5041 aatgttataa gtttcacccg ctttaccttc ggttacgacg gtatataacg cacgagcatg
     5101 atcctctaca tacaaccagt cgcgaatttg attcccttta ccataaatag gtaatgcctt
     5161 accttccaga gcattcagaa taaccaatgg aataagtttt tccgggaagt gataaggacc
     5221 ataattgttc gagcaattag tcacaatggt tggtaaacca taagtccgct tccaggcacg
     5281 taccaaatga tcgctggaag ctttagacgc agaatacggg ctacttggcg cgtaagctgt
     5341 cgtttccgta aatagcggta acgcttcgtt attatttgct tcatccggat ggggtaagtc
     5401 accataaact tcatcagtag aaatatgatg aaagcggaaa ctttttttct tttcatcatt
     5461 cagagcagac cagtaattgc gcgccgcttc taaaagaaca taagtcccca caatattggt
     5521 ttcaataaat gccgcagggc ccgttattga acggtcaacg tggctctctg ccgccaggtg
     5581 catcaccgcg tctggctggt gctgtgcgaa aatacgcgac atggctacag catcgcagat
     5641 atctgcatgc tcaaaagcat aacgttcaga atcagaaaca tcagcgagtg attccaggtt
     5701 cccggcgtat gttaatttat cgacattaac aacactatct tgcgtattat ttattatgtg
     5761 acgaacaaca gcagaaccaa taaaccctgc gccaccagta acaagtatct tcactttttt
     5821 attccatata gctatagagc atgctgtgaa atagactgct ccagatttga ttaatagagg
     5881 tattaataca cgctaccgcc cctggcttaa cagctaccag tgcactgcat gcatctcaac
     5941 gatgtggcga gcgttagcca ctcgcgccaa atccgaaaaa ttcaaacgtt aattgtctta
     6001 ccaaacagcc gtagaaacaa ggaaaatcct ggaaaaattt gaataatatg agctatgtaa
     6061 cttcttgttt ttataggctt tataaaaacg gcaacggcag gaaaattcaa tgagcaaaac
     6121 ttatgccacg aatcttcact gccgttataa ttttcttatc aaccgttaca tccggtcaga
     6181 ttttcattat tcgcttaaca gcttctcaat acctttacgg aacttcgccc cttctttcag
     6241 gttgcgtagg ccatacttca caaacgcctg catatagccc atttttttgc cgcagtcgta
     6301 actgtcgccg gtcatcagca ttgcatcaac ggattgtttt ttcgccagct cggcaatagc
     6361 atcagtcagc tgaatacgtc cccatgcacc aggctgagta cgttccagtt ccggccaaat
     6421 atcggcagaa agcacatagc gacctacggc catgatgtct gagtccagcg tctgcggctg
     6481 atccggtttt tcgataaatt caacaatgcg gctgacttta ccctcacggt ccagcggctc
     6541 tttagtctgg atgacggagt attcagagag gtcacccggc atacgttttg ccagcacctg
     6601 gctgcggccc gtttcgttga aacgtgcaat catggcagca aggttgtaac gtagcgggtc
     6661 ggcgctggca tcgtcgatca caacgtctgg cagtaccacg acaaatgggt tgtcaccaat
     6721 ggcaggtcgc gcacacaaaa tggagtggcc taaacctaaa ggttcgccct gacgcacgtt
     6781 cataatgatc acgcccggcg gacagatgga ctgtacttcc gccagcagtt gacgcttcac
     6841 gcgctgctca aggagtgatt ctaactcata agaggtgtcg aagtggtttt cgaccgcgtt
     6901 cttggacgcg tgagttacca ggaggatttc tttgatccct gcagccacaa tctcgtcaac
     6961 aatgtactga atcattggct tgtcgacgat tggtagcatc tctttgggta tcgccttagt
     7021 ggcaggcaac atatgcatcc cgagacccgc tacaggaata actgctttta aattcgtcat
     7081 tatttcatcc acctgtaaaa tggttgctga attatagctt gttcgatttt tttcgccagc
     7141 atcaattacc ctgaattgat tactgaatta cttgtgatgt tacgccgctt cgttgtggat
     7201 tgcagtagca ttgttcctaa gtatggctcc atttttccag gaatggtcgc aaatctactc
     7261 cctccgttcc ggcaatctaa agttaatctt ttccacatta acaatatggt gattaatcct
     7321 gtcgatatcg acggagcttt gccctctttc attcaccgca tgaacatttg caagagacag
     7381 cagtgtttct tttttcgcca taaagacgcc gcgaacgtct ttgcgcatgt cgaagttcat
     7441 gctcaatgct ggtccaactg aggattcctg catcacattg atatttcgca taaaaagatg
     7501 ttgcggcttg ttgtgtaact ccagcgaggc acgcttcatc tcaatgttag ttagcgacac
     7561 aaaggagacg gcattcccgg cggagatttg gatgccgcgc aatttataag caagatgggt
     7621 attatccagt tgaatattat tcactcggaa attttgcggt atcgagagat atttgccttt
     7681 aattacccca tagccgatta acatcccggc gctattaatc atttcaatat tatcaatcac
     7741 gaaattgtca caaccgtaaa tagcgactgt cgcgttatca atgcccgctt tcttactgaa
     7801 atccggcgtg atattgcggg cattgatatt acgaataaca aaatgtttac cattttcaac
     7861 atggatcaac tgccgacaat ccgatcccgt gatattcgcc acgacaaagt ttttcactgc
     7921 ctggtcttcc gggtagttgt tatcataagt gcttcccgca aggcctatgc cgatgcccca
     7981 gttgattttg ccgttggtac agttgatacg ctcgatgaca tggtcagata tcaaaatatc
     8041 actgtcgtta attgccacgt tccattcaat ggcgtcgccc tgtaagtcgc tgaacttaca
     8101 gttggtaatg ttggcaccga taatctggtt atgaaatccc tggcgtaaga tggcgtaatt
     8161 agcgtggctg acagtgaggt tatcgatggt caggttgcgc atgacccgtt tgtttttgcc
     8221 gccaatataa atctgcatta ccgggccaaa gccgctcatc gccagccctt tgatggtgca
     8281 gtcagaacca cgcacatcca gggtaatgtt atgcataccg ccgccctctc cccctgtcac
     8341 ctggctgccg tcctgtaaga caaatcgccc tctgccgttg ccgcgcaggc ttccaaggat
     8401 gtgtaacgtt ttgccgggag ggatgaagat gccggtgttg atattgtcac aaacgaatcc
     8461 ggcaggcacg acgaccgttt gcccttcgct gaaggcttgt ttaaatgagg cgatccagtc
     8521 atgtgggttg tagtcgttaa tgttaacgct ttgtcgggcg ggaagtgcgc gagcgaaagg
     8581 ggtatggagg aaggcaagcg ccgagcttgc cgtcaggaag gtgcgtcggg agagtgtttt
     8641 aaatggcata cgttctcctc tataaagcct gcagcaagct ggcgagttct cgattaatca
     8701 cctgctggtt aaaatcgtgt tcgacttttt cgcgcgcacg tttgacgacc ggagccagtt
     8761 cgtcggtatc cagttggcta aacgccgcca gacgttgtgc cagtgcgcga gcatcgttct
     8821 caggcagcag ccagccggat ttatcggcct ccaccagttc aggaattccg ctatgcagag
     8881 tagaaaccac cggaataccg accgccatcg cttccattag cgccaccgga atgccttcca
     8941 tatcgccatc cgcgcccgtt accgatggca acaggaagac atccgcgtcg tcgagcatct
     9001 ccttcacttc atggctcggt ttaaagcccg gcatctctac cacatcttcc agttgatatt
     9061 gttcgatgag ggtgcgcagg cgtcgttccc acaggccaat gccgaggatg cgatagcgaa
     9121 atgccacgcc ctgctctttc aactgacggc aggcttcaat cgccacatgc aggccttttt
     9181 tctcggttaa gcgtgcgaca gatatgattt ccagcggcgt tgcgggcgct ttcaccggac
     9241 gcgggctaaa gcgcgtcatg tccacgccca tacgcgatac ggcgattttt tctctggggc
     9301 agcccatttt ttgcagcctt ccggcccaca gatcgcttat cggtaacatc aggtcgccac
     9361 ggcgaaacag ttgttgatat tcgggagtgt agtggttgag cacttcccga ctggagatat
     9421 caataccgtg gaagatggtg gcaattttgc cgcgaatgat acccagttcg cgtagttttg
     9481 ctgcggttac ccccgccggg ccaaaatgag cgatgaacac atcggcgcga aacggtgttg
     9541 cgacctgacc acaaatggca gacaaaatca ggttccgcga ctcggcacca tagcgtttga
     9601 ggttgagcgc atgccaggta tttttacgat gaatgccacg taaggtctgg ctggcgcggt
     9661 ggcgcagttt cgctactttg cccgcaggtt cgtcctgtaa ccagcgggtt ctggcgacaa
     9721 ggttgtattt cgtccatgcc gcgtgggtgt tctctgtatc gcctttttgc aaagcgacaa
     9781 tctccacttc aaagcccata tcaataaacg cggtaatttg attgaggacg aaggtttccg
     9841 acgacagcgg gaatttcaat aagaagaagc cgaccttcat ttcacctccc cgatgcgctc
     9901 aagcacagac tgcaccatct gcattcccgt ctgacgttcg cgactgacgg cttcgttaaa
     9961 tcgagcgttc agcaccggaa gctggcctaa ggtatccgca accatcgctt gcaggctgcc
    10021 gtccagtaaa tgacgaatat caatcgccat ctctggcagt cccagctgct gcataatccc
    10081 ggcggattta tgttcatagt tgatggcaat cgccggagta gcaaagttca ttgagataat
    10141 ggcggagtgc aggcgcgtac cgacggtgag ttcacaggct ccgagaattt tgcccatttc
    10201 cagatcgttg agttcatcca tcactacgtg gtaacgggca ggatcgctga tgtgctggcg
    10261 cagattgagc gccaccatgc ggtcgtcttt gttatagctg tcgatacccg tacaggtgga
    10321 gagcgcaatc acctgatagc cttcatcgag aatgcgattg accaccccgg caaaggcttt
    10381 ttcatacgct tgttgagtgg tgccgagacg tttgtcgaac ggtgccagtt cgcgcagggt
    10441 aattgccact gttttctgtt gtgcggcaac gtccagccag tgttgaacgg catagctggc
    10501 ggtgaagtct tcggtgtggt gatcgaccag ccacgcggta tcgacgccat gttccacttt
    10561 tgcggtggtg atatttctgc gtttcatcaa atcaaggctg accgattcgc gcaggatcag
    10621 cgcgtcgcag tgaccaaaga catagttcgc cagttggtta aattgctcat cctggaaggg
    10681 gccgacgctg tgaccaatca taaacagcgg ctttttcgcc ataaatgtgc aaagcgcatg
    10741 ttcaaactgc ggcacgccgt agagatcgac aaaaaacgat ccgccgacct gaataatggc
    10801 gtcgtaacct gacagcaggc gcacgaagtc ggtgaatccc tgggcgatgg cgatattgcg
    10861 cagcttgcca gtgtcggtga cgcgtgagag caatacctga tgctggtagc ggcgacggag
    10921 gactttttta atgcgcccga caacgcccgc cgcgctattg tgttgtgtca tctgcaggaa
    10981 cagcggatcg cccattaccg ggcggttgag cagccaggaa gaactgaccg gatagcggct
    11041 catcacgtcc acttcggtat gtggattgag gatgttgatg gcatcaagta agccgcgcaa
    11101 gatggcgctg tcgccacgat tgccgcaagt gtggttgccc agaataagta atttcatttt
    11161 ttcctcataa atttgatgcc aggtgaggct gtgtttgttg tgttcgttgc cgttttttgc
    11221 ctgatgcgac gctggcgcgt cttatcaggc ctacaggtcc cgagcacaga accgtaggac
    11281 ggataaggcg ttttacgccg catccggcaa ccgttgtcgg aaccgaaaac agcaattcac
    11341 cccgcccgta aaagcatttt cattttttcg ctgcgacaaa actgacgctt cacttccacc
    11401 accagcggat ggcgtgacag cacaatcatc accacaaacg ccagcacgcc cgcggctatt
    11461 tgcaccgcca gcagcatccc cagcgccagt tgccctttca gcacaatgcc cagcgcataa
    11521 ctgaccacca gcgtcggcaa cgagagataa aacggcagcc acaaactcag gatgtactgg
    11581 cgataactgg aaccaagaac cggtttaatc atcacgaagt aactcagaat ggtgttgata
    11641 atctgcacca gcaggaaacc gagcgtgacg ccgattgcgc ccgccatctg cccaccaata
    11701 acaatcgccg gaataaacag aaatgttttg aatacgttga atttaaagct gatatcgacc
    11761 cgcgctttcg ccatcagcag cgaaccaatc gggttaccca cggagcgcag cagacccaca
    11821 atacacagca attgcagcac cggaataatg ctgttccact tttcgccgaa taccagcggt
    11881 acgaagttat tcgacaccac catcagcccc agcagcgccg gaaagttgat aatccccact
    11941 atcgacagca gcttgtagaa gttaacacgc agcttttcgg tgtcgtcctg aattttggca
    12001 aacgccggaa acaacacgcg ggtgatgatt gggttcagct tcatcggcgg tacgacggcc
    12061 acgttgtacg ccaggttgta tccccctgcc acgcccgcgc caagaatacg cgccagcacc
    12121 agcgttgaaa ggttggtgtt gagatagttg atgatgctgt ccgccgtcag ccaggcacca
    12181 aaacgtaagt tcggtgccac agacgccagc gagaagtgca gaccggggca ataaattttg
    12241 cgtccaaagt agccaaacag cagtgttctc accgcactat tgaccagata accgaggatc
    12301 gcggtcatcg ccagcggcca gaaatgggcg ctaactaccg taaaagtgaa gcccgccagc
    12361 accgcgctgg tttcgatcat gccgattttg ttgaactcca gctccttctg catcaacgcg
    12421 cggaactgtt gcccgtgggg gatcaccaca aacgccagcg acaatgtttt aatcaacggt
    12481 gccaggtccg ggttattcag cacgtcgccg atgacatcac tcaacaaaaa caccgccaca
    12541 cataccacaa gccccagccc gacgttcagc cagtacagcg tggtgagttc aaggtgactg
    12601 atttcttttc gctgaataat cgagttagcg ataccgaagt cagaaagcgt atctgccagc
    12661 gcgataatca ccagcgacac agtcagcagg ccgaactggt ggttgtcgat aatccgcgcc
    12721 agcacggtca tctgcaccag cccgaggccg atgatgatca ccgtggcaat agccgaccac
    12781 ttcgcgccgc tgatggtttt ttcacgtaag ctcatatcaa tatgccgctt tgttaacgaa
    12841 gcccttgaat accgtcagga aaacgatttt gatatcgaac cagacgctcc attcgcggat
    12901 gtactcaagg tcgaactcga cgcgtttttc cattttctcc agcgtgtcgg tttcgccgcg
    12961 ccagccatta atctgcgccc agccggtaat gcccggtttc actttatggc gcagcatgta
    13021 gccttcaatg agctggcgat actgttcgtt atgcgctact gcgtgcggac gtggaccgac
    13081 aatcgacatc cccccggtca gcacattgat aaactgcggc aattcgtcca gcgaggtgcg
    13141 gcgcagaaag ttccccactt tagtgacgcg cggatcgttc tgcgtcgcct gggtcaccac
    13201 tttgtcgttc tccatcactt tcatggaacg gaacttccac actttgatcg gcttgccatc
    13261 cataccatag cgagtctggc ggaaaataac cggccctggt gaactgagtt tcaccgccag
    13321 cgcaatacag cacagcaccg gggagatcag cagcagaata agcgtcgcca gcacaatgtc
    13381 ttccgcacgt tttagcaggc ggttaacccc cgaaagcggc gtgtcataca gcggcaccac
    13441 cggaacgccg ttcatctctt cgagacgtga atggagaatg ttgaaggtaa agacgtcggg
    13501 gatcagcagc accgaacagg tggtatccgc cagttgatgg accagttttt tcactcgtgc
    13561 gccgtcgcac atttgcatcg cgatatagac gttatgaatc ttgcccgctt ttgcatcctc
    13621 gaccagctgt tgcaggttac ccgcccagtc gttagaaacg ccgcccggtt tcgggtcgtg
    13681 gtaaacgccc accacttcaa accctaacca cggctgatta cggaaactct ccatcagcat
    13741 ttgtccggcg gctaaatccc ccgctacggc gaccatccgc ttgttgtagc catgattacg
    13801 cagccagccc gccccaatgc gaatacatga acggcaaacc accagtccga tgctggtcag
    13861 cccataccac gccagccaga ttttcagttg cgtgtcgaaa tcattgttga acgccaccag
    13921 tccggcgctg aaaatcacgc ttaaggtcca gttttgtagc aacagggcaa attctgtcgc
    13981 tgcccgaaca ccgcgccatg agcgataaaa atcggtgatg ccgcccagca tctggaacac
    14041 caccagcgta atcagcgcca ccaacaggtg catgtagagg aatgacagtc cgctgacttc
    14101 gcaaaccagc catagcccgg caaacatgat ggtgatatct gaaaagcgtt gcaccataga
    14161 gattaacgat gcattggttt tcgctcgctc gcgctttttt agatttgtca tcgttgttcc
    14221 tgttattagc cccttacccg cagtggggta agggaagatc cgacattact cgttcagcaa
    14281 cgtcagcaga gttcgcgttc gcgcttccat cagcggcaca tcaccgcgcg attccacatt
    14341 caggcgcacc accggttcgg tattggaggt gcgcaggtta aagcaccagt cggcaaaggt
    14401 catgctgatg ccatcgctgc gatccaccgc cagcgcctca cggctaaaat gctgttccac
    14461 gcggttaatc gcctcaacgg gttgcgccag tttgctgttg atctcaccgc ttgccggaaa
    14521 cgccgccatc cggtcgcgca ccagttcgcc cagcgttttt cctttcaggc acaccagttc
    14581 ggcgaccagc agccacggga tcatgccgct gtcgcagtaa gcgaaatcac ggaagtaatg
    14641 gtgggcgctc atttcgccac cgtagatggc gtcttccttg cgcatccgtt ctttaataaa
    14701 ggcgtgtccg gttttcgaca ttaccggcgt gccgcctgca gcagtcacca catcaatagt
    14761 gttccaagag agacgcggat catggatgat cttcgcgccg ggattttttt cgaggaacgc
    14821 ttctgccagc aggccgacaa tgtagtagcc ctcgataaac tgcccttttt cgtcaaacag
    14881 aaaacagcgg tcaaaatcgc catcaaaggc aatgcccata tccgcgccgt gtttgatgac
    14941 cgcattgcgg gtgtcgtcgc gacattccgg cagcagcggg ttaggaatac cgttggggaa
    15001 attgccgtcc ggtgtgttgt gtactttgat taattccacg ggcgcgccga gggctttaaa
    15061 gcgggcttca atggcgtcca ccaccggacc cgctgcgcca ttcccggagt tgatcaccag
    15121 cttgagcggc gtgagatttt tgacattgat ataaccgaac aggtgatcaa cgtaagcgtc
    15181 acgcaggttg atttgctgat agcgaccgcg tttggtttca tcgacgggag gaaagtcgtt
    15241 ggcttcagcc agacgctgga cgtcgcgcag tccggtatca ccgctgatcg tgcgagcccc
    15301 ctcgcgcacc agcttcatgc cgttgtaatc catcggatta tggctggcag taacttcaat
    15361 gccgccatcc acgccgagat ggaacgtggc gaaatagatc tcttcggtgc cggacatacc
    15421 gatatccagc acatcgacgc ccgcatcctg taaacctttt gccagcgcca gttttaaggt
    15481 ttcgctggtg agacggacat caccgcctaa cacaatggtt ttcggtttga gaaattcgcc
    15541 ataggcgcga ccaatgcgcc aggcgatatc ttcattcagt tcttcgccta atttcccgcg
    15601 aatatcatag gctttaaagc aggttaattt tttcatgtcg ttaccctttt tcagacaata
    15661 gttggccctg accgaaacgg ccaattttat ttttgtcgtt atttacaccg cggtttcgca
    15721 ttcattgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaaccga accgtaggtc
    15781 ggataaggcg ttcacgccgc atccgacaaa cagcgcctga tgcgacgttt acacccgtcc
    15841 gtagcgatcc gcgaagcgca ccacatcatc ctcttcgaga taagagccgg agcgcacttc
    15901 aattaaatcg agcggaattt tccccgggtt ttccaggcag tgcgtcgccc ccagcggaat
    15961 ataaatggac tcgttttcac caagcagttt gatatcacca tcaatggtga cttttgccgt
    16021 tcccgcgacc actacccagt gttccgcgcg gtgatggtgc atctgtaccg acaaaccctc
    16081 gcccggtttc acggtgatgc gtttcacctg gtagcggtcg cccgcgtcga tagagtcata
    16141 tttgccccac ggacgataca cttcgcgatg cacccgatgc tcatggcgac catcggcttt
    16201 gatctgctcg accacttttt tcacatcctg taccgcgtta cggtcggcaa tcagcaccgc
    16261 atctttggtc tgcaccacta ccagatcttt caccccgacg gtggtgacca ggcctgattc
    16321 ggcatacaca tagctgtttt cagttttgtg attaatcaca tcgccgtggc aaacgttgcc
    16381 ctcggcggtg tgggcgctga tctcccataa tgaagaccag gaaccaacat cgctccagcc
    16441 cgcatccatc ggcaccacaa cagcatctgc cgtaggttcc atgaccgcgt aatccaccga
    16501 ctcttccggg caggcgagaa acgcttcttc atccacacga ataaaatcga gatccggatc
    16561 gacggcgctc atcgcttttt cgcaggcatc gaggatatcc gggcgatatt ttttcagttc
    16621 ttcgagatag cgtccggcgc ggaacaggaa catgccgctg ttccagtaat attcgccgct
    16681 tgctacatag gcctgggcgg tttccagatt cggtttttcg acaaactgcg ccacttcaaa
    16741 ggccaccgta tcctgctcac ccacagacac ttcaccgcga cgaatatagc cataaccggt
    16801 ttctggtaga tccggcacaa tgccaaaggt caccagcttg cccgcttcgg cataaggcat
    16861 ggcattacgc acggcggcac ggaacgcgtc ttcatcggca atcacatgat ccgccgccaa
    16921 taccagcatt aacgggtcgc tctccgggct atgacgtttt gccgccagcg ccgccagtgc
    16981 aatggcaggt gcagtgttac gccctgccgg ttcgagaata atgttctcgg tgagtttgtt
    17041 cagttgacgc agctgttccg cgacaataaa gcggtgctgc tcattgcaaa tcaccaccgg
    17101 gctttcgcac tccacgccgt tcaggcggca gatggtggtt tgcagcatgg tgagatcgcc
    17161 tttcaggcat aaaaactgct tggggtaaag tacgcgggaa agcggccata aacggctacc
    17221 ggagccacct gccatcacaa ctggatagag tttcgactgc gccataatta tccccgaata
    17281 tcatttataa attgacgtaa cacgttctct ttatcgagcg tgcgttcggc atattcacgt
    17341 gccaccgtgt tatgtttggg cagcaggagc gcctgacgaa tccccgccac cagcgcctcg
    17401 accgattccg gttctacgca aaccgcaata cccggaaagg tttcgcaaag ctgccccagt
    17461 tctgtgtggg cctctgcggt aatcaccgcg ttaccgccta ccgccagaat attggtcagt
    17521 ttcgacggca atacggcatc tgccgcgccg cgtttttgca ccaccagatg gcaatcgccc
    17581 atcttcagca gtgcaggtaa agcgtcatac gattgcagcg ggaaaaattg catgttgcgc
    17641 agtccacgct gctgcgccat tttttccagc cgcgctttgc cgccgccctg cccgacaatg
    17701 gcaaaaatca gcggttcatc gcgcaggaga tcggcagctt caataacgtt ttccagcccc
    17761 tgcttttcac caatattgcc ggagtaaaga atgatttttt tgttatccgg caggccaagc
    17821 tggttacgaa gggcatcaac atcggcgtct gcaacatgct gaaaacgggc aatttccgac
    17881 cagttgggga agaagatgac gttatccgcc gccacgcctt tttcgatggc tttattcatc
    17941 atcgaacgcg aaatcgtgga aacgttatcg acgttatgca gtccgctacg ttcgaacgcc
    18001 gttaccagct gtgccacttt gccgcctttg ccttttccgg caaggcccag ccccagcatg
    18061 gcgtccactt cgtaatcctg aatatgcagc acggtacgcg caccagagag cttcgccagc
    18121 aggcgcattc ccggcgtgca aaacagcgtt ggcactacgc caataatgcg atccggcttc
    18181 cagcgacgtt gcgccatcag tggaaagaaa ctgctgacgg caaaactgcc gagatgcaac
    18241 aagcgtttca gggtgctcgg ctgttttggc acgtacagcg ggcagcgcca caccgtggca
    18301 gccccttctt ctcgtttgta gcgccaggcg gaatagttct cacccacctg ccattgcggg
    18361 tagtaaggcg gtgcggtaat gacccgcacc tcatgacctt gtgccgccaa ccattccacc
    18421 atctcgccgg tgtatttgcc gatgccggtt aactccggcg agtagttaat gccgtagacc
    18481 agtattttca taatccgggt actccggcac gcttctcagc gagaaaatag gctcggctgt
    18541 tagcgtgaac atcgttactg gcgagcagcg cgtccggcgt cagccagcgg taatcgtcat
    18601 gctgctcatc cggcagtaac agctcttctt ccgctactct gaagcgaaaa ccgagcacca
    18661 catagtgagt ggtgaaatcc gtgccggaga agttatcgtc ataaaaatgc tgccagacac
    18721 cgtaaaactg gcctgctgtt atcggcagac gcagccccag ttccgccatc gtcagccgct
    18781 caaatgcggc ttccagcgtt tcgtctttct gcacgcgccc tcccggcaca aaccagtaac
    18841 cctgcgccgg gcggttggtt cttttgccaa gcagaaactc gccgcgactg ttctcgacaa
    18901 taaagtcgag agagacaagc ggtgtggagc gcactacagt ggcaaagtct tcctgacgta
    18961 aaaacatcat tccccccgaa agcggtcttg attctcaagg aaccactggt aagtgctggc
    19021 aagccccgct tccagtgaga tttcgtgata ccagccaagc taatgcaggc gcgtcacatc
    19081 cagcagtttg cgcggcgtac catccggttt gctggcatca aaaaccaccc gacctttgta
    19141 acccaccact ttggcgatgg tttgcgccag ctcgcggata gtgcagtcaa cgcccgtgcc
    19201 gacgttaatg tgcgacagca tcggttgggt attctccagc cagacttcat gcgccagctc
    19261 catgacatga atgctcgccg ccgccatatc atcgacgtgc aggaattcac gcatcggtgt
    19321 accgctgccc cataccacca cgtccggcgc attctgtgcc gtcgcctcgt ggaagcgacg
    19381 cagcaatgct ggtatcacat gggagttact cgggtggaag ttgtcatgcg gcccgtacag
    19441 gttggtcggc atgacagtgc ggtaatcgcg tccatactgg cggttgtaag attcgcacag
    19501 tttgatcccg gcgattttgg caatggcgta aggctcgttg gtcggctcca gcgtgccctg
    19561 caataactcg ctttctgcca tcggctgttt tgccagtttc gggtagatgc aggacgatcc
    19621 gagaaacatc agtttgttca cgtcgttctg atgcgcggcg tgaatgatgt tgctctcaat
    19681 catcatgttc tggtagatga aatccgccgg ataggtgttg ttggcaacaa tgccgcccac
    19741 tttcgccgcc gccagataga cctggtcaat gcgttcgctg gcaaagaaat catgcacggc
    19801 gcggctgtcc agcaggttca gttcatcgcg ggtacgtaat accagctcca catcaccgcg
    19861 ctgttcgagc tgccgcttga tggcagaacc gaccatccca caatggccag cgataaaaat
    19921 gcgttgttta ctcatgctta tgactccagc gcgatcgcca cgtcgtagcc gtgagatttc
    19981 agcagagagt gttttttcgc cgcttcgagg tcattagcca ccatttcaga caccatctct
    20041 ctgagggtga tttccggttt ccagcccagt ttttcgtgcg ctttggtcgg gtcgccgagc
    20101 agcgtttcaa cttctgccgg acggaagtaa cgcggatcaa cggcgataat cacatcaccc
    20161 ggtttaacgc ccggcgcgtc atgcccggtg acggaaacca caatgccctt ctcttcaaca
    20221 cccgtgcctt caaagcgcag tttgatgccc agctgtgctg ccgccatttc cacaaactga
    20281 cgtacggagt actgaacgcc ggtcgcgata acgaaatctt ccggctgttc ctgttgcagc
    20341 atcatccact gcatttttac gtagtctttg gcgtggcccc agtcacgcag ggaatccata
    20401 ttgccgaggt acaggcacga ctccagcccc tgggcgatgt tggcgattgc gcgggtgatt
    20461 ttgcgggtaa cgaaggtttc gccgcggcgc ggggattcat ggttgaagag aattccgtta
    20521 caagcgtaca tgccgtagga ttcgcggtag ttaacggtga tccagtaggc gtacagtttg
    20581 gcgaccgcat acggagatcg cgggtagaac ggcgtggttt ctttctgcgg aatttcctgc
    20641 accagaccat acagttcaga ggtggaagcc tgatagaaac gagttttctt ttccagaccg
    20701 aggaagcgga tcgcctccag caggcgcagc gtacccatcg cgtcgacgtc agcggtatat
    20761 tctggtgact caaaagagac cgcaacgtgg ctcattgcgc ctaggttgta cacttcatcc
    20821 ggctgtactt cacgcaaaat gcgcgtcagg ttagaggtat cactcaggtc gccataatgc
    20881 agatggaatt tcgggttgca ggtgtgcgga tcctgataaa tgtgatccac gcgctcggtg
    20941 ttgaatgacg atgcgcgacg cttaatacca tgcacctcgt aacctttttc cagcagaaac
    21001 tctgccaggt aagaaccgtc ttgtccggtt acaccggtga tgagagcgac ttttgacatg
    21061 tcttattcct ctgtattttt gaatttattc agtttcaacg cgttcgcgta tcaccactgc
    21121 ggggttcccc cggcaaacca catttgccgg aagcgattta aaaacactgc ttcgtgcacc
    21181 cacgacggtg ccgtcgccga ttgtgacgcc tggggcaaca aagacatcgg ttgccagcca
    21241 gcatttctcg ccaatcacaa taggcgtggc gttaatggtg aaatgttgac ttgcatggtc
    21301 gtggctaccg gtgcataaat aacttttttg cgatatcacc gaatgtgcgc caatggttat
    21361 ttcaccgagg gtatataaat tgacgtcatc gccaacccac gcgtaatcac ctaaggttaa
    21421 tttccacgga taggtaattt ttactgacgg acgaataact acgttttttc ctatttttgc
    21481 tccgaataaa cgtaataaaa aagcccgcca gcgatacaat acttgtggcg accaggcaaa
    21541 tattgttgcc tgtactgccc accataattg cactttaata gcgttgccgc cccggaaccc
    21601 tttcggcacc gagaaaccgc ttaaatcttg cattggtttt ccttatattc agactttgtt
    21661 atataaggct ttagtcttac ctgtcgtacg ttggcgtaaa tgccaggata attctgccca
    21721 gaagccaggt acatgtaata tctgccgttg gacttttttg gcgtcagcac
//
LOCUS       DAECOD010000063        21518 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.063, whole genome shotgun sequence.
ACCESSION   DAECOD010000063 DAECOD010000000
VERSION     DAECOD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.063"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KKO15_002460"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KKO15_002460"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBD6361215.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="KKO15_002461"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="KKO15_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBD6361216.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="KKO15_002462"
     CDS             complement(2649..3200)
                     /locus_tag="KKO15_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBD6361217.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="KKO15_002463"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="KKO15_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBD6361218.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="KKO15_002464"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="KKO15_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBD6361219.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KKO15_002465"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KKO15_002465"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBD6361220.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KKO15_002466"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KKO15_002466"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBD6361221.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSIFTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KKO15_002467"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KKO15_002467"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBD6361222.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KKO15_002468"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KKO15_002468"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBD6361223.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KKO15_002469"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KKO15_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBD6361224.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="KKO15_002470"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="KKO15_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBD6361225.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="KKO15_002471"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="KKO15_002471"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBD6361226.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KKO15_002472"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KKO15_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBD6361227.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KKO15_002473"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KKO15_002473"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBD6361228.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KKO15_002474"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KKO15_002474"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBD6361229.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KKO15_002475"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KKO15_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBD6361230.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="KKO15_002476"
     CDS             complement(18174..18569)
                     /locus_tag="KKO15_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD6361231.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KKO15_002477"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KKO15_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6361232.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="KKO15_002478"
     CDS             19485..20078
                     /locus_tag="KKO15_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD6361233.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="KKO15_002479"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="KKO15_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD6361234.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21518
                     /locus_tag="KKO15_002480"
     CDS             20798..>21518
                     /locus_tag="KKO15_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6361235.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5202 a         5325 c         5665 g         5326 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctacggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtaaagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacatgttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc tgggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcgtc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaata
//
LOCUS       DAECOD010000064        21499 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.064, whole genome shotgun sequence.
ACCESSION   DAECOD010000064 DAECOD010000000
VERSION     DAECOD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.064"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KKO15_002481"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KKO15_002481"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBD6361236.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGIIV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KKO15_002482"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KKO15_002482"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBD6361237.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="KKO15_002483"
     CDS             complement(3122..3421)
                     /locus_tag="KKO15_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBD6361238.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="KKO15_002484"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="KKO15_002484"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBD6361239.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="KKO15_002485"
     CDS             complement(4633..5070)
                     /locus_tag="KKO15_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBD6361240.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="KKO15_002486"
     CDS             complement(5116..5457)
                     /locus_tag="KKO15_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361241.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KKO15_002487"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KKO15_002487"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBD6361242.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KKO15_002488"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KKO15_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="KKO15_002489"
                     /pseudo
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="KKO15_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="KKO15_002490"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="KKO15_002490"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBD6361243.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="KKO15_002491"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="KKO15_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBD6361244.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSTRNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13218
                     /locus_tag="KKO15_002492"
     CDS             12688..13218
                     /locus_tag="KKO15_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD6361245.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13330..13866
                     /locus_tag="KKO15_002493"
     CDS             13330..13866
                     /locus_tag="KKO15_002493"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD6361246.1"
                     /translation="MIGIDNEELTRYLSRIALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPDVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13974..14798)
                     /locus_tag="KKO15_002494"
     CDS             complement(13974..14798)
                     /locus_tag="KKO15_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBD6361247.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="KKO15_002495"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="KKO15_002495"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD6361248.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="KKO15_002496"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="KKO15_002496"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBD6361249.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="KKO15_002497"
     CDS             complement(18730..19203)
                     /locus_tag="KKO15_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBD6361250.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="KKO15_002498"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="KKO15_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBD6361251.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20354..21346
                     /gene="yiaK"
                     /locus_tag="KKO15_002499"
     CDS             20354..21346
                     /gene="yiaK"
                     /locus_tag="KKO15_002499"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBD6361252.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21358..>21499
                     /locus_tag="KKO15_002500"
     CDS             21358..>21499
                     /locus_tag="KKO15_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD6361253.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5402 a         5490 c         5277 g         5330 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacgataata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgagttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcacc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtatcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgacgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgaccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg ggcaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg acgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacgcctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggcaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaac
    20341 aaggaagcct aggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
    20401 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
    20461 caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
    20521 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
    20581 tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg
    20641 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca
    20701 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
    20761 ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcatcggcac
    20821 taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtt
    20881 ctcttacggc atgttagaag ttaaccgcct ggcaggccgt cagctcccgg tcgatggtgg
    20941 ctttgatgat gagggcaatt tgaccaaaga acctggcgtt atcgagaaga atcgccgcat
    21001 tttgccgatg ggctactgga aaggttctgg catgtcgatt gtgctggata tgatcgctac
    21061 tctcctttcc gacggcgcat cggttgccga agtcacccag gacaacagcg acgaatacgg
    21121 catttcacaa atttttattg ccattgaagt ggacaagctt atcgacggtc ccacccgcga
    21181 tgccaagctg caacgtatca tggattacgt tactaccgct gaacgcgctg acgaaaacca
    21241 ggccatccgc ttacccggcc acgaatttac taccctgctg gccgaaaacc gccgtaacgg
    21301 catcactgtt gatgacagcg tgtgggccaa aatccaggcg ttatgaggag ataagtcatg
    21361 atttttggac atatcgcgca gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg
    21421 ctcgattttc tgcgcgctac caacttcaac gtcctggagc cgggcgttgt cgaaatcgac
    21481 ggcaagaata tttatgcgc
//
LOCUS       DAECOD010000065        21256 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.065, whole genome shotgun sequence.
ACCESSION   DAECOD010000065 DAECOD010000000
VERSION     DAECOD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.065"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            21..794
                     /locus_tag="KKO15_002501"
                     /pseudo
     CDS             21..794
                     /locus_tag="KKO15_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="KKO15_002502"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="KKO15_002502"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBD6361254.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="KKO15_002503"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="KKO15_002503"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBD6361255.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="KKO15_002504"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="KKO15_002504"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBD6361256.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /locus_tag="KKO15_002505"
     CDS             complement(4992..7226)
                     /locus_tag="KKO15_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBD6361257.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="KKO15_002506"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="KKO15_002506"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBD6361258.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="KKO15_002507"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="KKO15_002507"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBD6361259.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="KKO15_002508"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="KKO15_002508"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD6361260.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="KKO15_002509"
     CDS             complement(12981..14321)
                     /locus_tag="KKO15_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD6361261.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NTGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="KKO15_002510"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="KKO15_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBD6361262.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="KKO15_002511"
     CDS             complement(16109..16558)
                     /locus_tag="KKO15_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBD6361263.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="KKO15_002512"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="KKO15_002512"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBD6361264.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="KKO15_002513"
     CDS             complement(17358..17687)
                     /locus_tag="KKO15_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBD6361265.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="KKO15_002514"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="KKO15_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBD6361266.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="KKO15_002515"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="KKO15_002515"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBD6361267.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="KKO15_002516"
                     /gene_synonym="ygdH"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="KKO15_002516"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBD6361268.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5669 c         5073 g         5288 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctgg tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctagtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagagct atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccgaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggtgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccataat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DAECOD010000066        21088 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.066, whole genome shotgun sequence.
ACCESSION   DAECOD010000066 DAECOD010000000
VERSION     DAECOD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.066"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(2..298)
                     /locus_tag="KKO15_002517"
     CDS             complement(2..298)
                     /locus_tag="KKO15_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBD6361269.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1229
                     /gene="tonB"
                     /locus_tag="KKO15_002518"
     CDS             513..1229
                     /gene="tonB"
                     /locus_tag="KKO15_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBD6361270.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPV
                     KKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(1269..1667)
                     /gene="yciA"
                     /locus_tag="KKO15_002519"
     CDS             complement(1269..1667)
                     /gene="yciA"
                     /locus_tag="KKO15_002519"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBD6361271.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1772..2311)
                     /locus_tag="KKO15_002520"
     CDS             complement(1772..2311)
                     /locus_tag="KKO15_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBD6361272.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2341..3084)
                     /locus_tag="KKO15_002521"
     CDS             complement(2341..3084)
                     /locus_tag="KKO15_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBD6361273.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3112..3201)
                     /locus_tag="KKO15_002522"
                     /pseudo
     CDS             complement(3112..3201)
                     /locus_tag="KKO15_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3441..4079
                     /gene="ompW"
                     /locus_tag="KKO15_002523"
     CDS             3441..4079
                     /gene="ompW"
                     /locus_tag="KKO15_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBD6361274.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4139..4645)
                     /locus_tag="KKO15_002524"
     CDS             complement(4139..4645)
                     /locus_tag="KKO15_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD6361275.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4691..5191)
                     /locus_tag="KKO15_002525"
     CDS             complement(4691..5191)
                     /locus_tag="KKO15_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD6361276.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5277..5456)
                     /locus_tag="KKO15_002526"
     CDS             complement(5277..5456)
                     /locus_tag="KKO15_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBD6361277.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5837..6643)
                     /gene="trpA"
                     /locus_tag="KKO15_002527"
     CDS             complement(5837..6643)
                     /gene="trpA"
                     /locus_tag="KKO15_002527"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBD6361278.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6643..7836)
                     /gene="trpB"
                     /locus_tag="KKO15_002528"
     CDS             complement(6643..7836)
                     /gene="trpB"
                     /locus_tag="KKO15_002528"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBD6361279.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7848..9209)
                     /gene="trpCF"
                     /locus_tag="KKO15_002529"
     CDS             complement(7848..9209)
                     /gene="trpCF"
                     /locus_tag="KKO15_002529"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBD6361280.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9210..10805)
                     /gene="trpD"
                     /locus_tag="KKO15_002530"
     CDS             complement(9210..10805)
                     /gene="trpD"
                     /locus_tag="KKO15_002530"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBD6361281.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10805..12367)
                     /gene="trpE"
                     /locus_tag="KKO15_002531"
     CDS             complement(10805..12367)
                     /gene="trpE"
                     /locus_tag="KKO15_002531"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBD6361282.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLC
                     AIATAHHAQETF"
     misc_feature    complement(12389..12482)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12459..12503)
                     /gene="trpL"
                     /locus_tag="KKO15_002532"
     CDS             complement(12459..12503)
                     /gene="trpL"
                     /locus_tag="KKO15_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBD6361283.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12641..13522
                     /gene="yciV"
                     /locus_tag="KKO15_002533"
     CDS             12641..13522
                     /gene="yciV"
                     /locus_tag="KKO15_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBD6361284.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13519..14139
                     /locus_tag="KKO15_002534"
     CDS             13519..14139
                     /locus_tag="KKO15_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBD6361285.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14240..15112
                     /gene="rluB"
                     /locus_tag="KKO15_002535"
     CDS             14240..15112
                     /gene="rluB"
                     /locus_tag="KKO15_002535"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBD6361286.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15152..15742)
                     /gene="cobO"
                     /locus_tag="KKO15_002536"
     CDS             complement(15152..15742)
                     /gene="cobO"
                     /locus_tag="KKO15_002536"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBD6361287.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15739..16497)
                     /locus_tag="KKO15_002537"
     CDS             complement(15739..16497)
                     /locus_tag="KKO15_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBD6361288.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16717..17766
                     /gene="sohB"
                     /locus_tag="KKO15_002538"
     CDS             16717..17766
                     /gene="sohB"
                     /locus_tag="KKO15_002538"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBD6361289.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17802..18053)
                     /locus_tag="KKO15_002539"
     CDS             complement(17802..18053)
                     /locus_tag="KKO15_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBD6361290.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18433..21030
                     /gene="topA"
                     /locus_tag="KKO15_002540"
     CDS             18433..21030
                     /gene="topA"
                     /locus_tag="KKO15_002540"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBD6361291.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         5289 a         5243 c         5439 g         5117 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccaaaaccga agccggtgaa aaaggtacag gagcagccaa
      841 aacgcgatgt caaacccgta gagtcgcgtc cggcatcacc gtttgaaaat acggcacccg
      901 cgcgccccac atcaagtacg gcaacggctg caaccagcaa gccggttacc agtgtggctt
      961 caggaccacg cgcattaagc cgtaatcagc cgcagtatcc ggcacgagca caggcattgc
     1021 gcattgaagg gcaggttaaa gttaaatttg acgtcacgcc ggatggtcgc gtggataacg
     1081 tacaaatcct ctcagccaag cctgcgaaca tgtttgagcg tgaggtgaaa aatgcgatgc
     1141 gcagatggcg ttatgagccg ggtaagccag gcagtgggat tgtggtgaat atcctgttta
     1201 aaattaacgg caccaccgaa attcagtaag cagaaagtca aaagcctccg accggaggct
     1261 tttgactatt actcaacagg taaggcgcga ggttttcctt caggatcaac cgctacatac
     1321 ttaaataatg cttctgtcgc tttatagcgt tgcccgattg gttcagacgc tacttttttc
     1381 acccatactt caatattaat gctgaccgat gtcgtccctt tctggacaca gcgtgcatag
     1441 cagcacacca catcgccgac cgcaaccggc cgtaagaaag tcattccttc aacccgcaca
     1501 gtcactacgc gaccgtgggc aatttctttt gccagaatag cgccgccaat atccatttgt
     1561 gacattaacc aaccaccaaa gatgtcacca ttggcattgg tatcggcggg catggctaaa
     1621 gtacgtaaaa caagatcgcc ctgagggacg ttatgtgttg tagacatggt aaaaccgact
     1681 taaaaggaaa atcacaggcg cgatgctact atgattttcc tgaggagaac agaggagata
     1741 cagcgccagc cccgaaggac tggcagtctg gttaggattt atcttcctgc ggcatgtggc
     1801 ggtagatata gataccgctt aacaatgtga agattaaggt aagggcggtc aggccaaaga
     1861 ctttaaagtt gacccaaata ttttgcggca gccagaatgc gatgtagatg tttgccagac
     1921 cgcaaaggat aaagaaaaca gcccaggcca gatttagctt cgaccatacc ggttgcggca
     1981 gcgtgagttc tttactcagc atccgctgaa ttagcggctt tttcatcacc cattggctga
     2041 ctaacagggc tcccgcaaac agagcataaa tgactgtaac cttccattta ataaactcat
     2101 cattgtggaa gaacagcgtc aagccaccga agacgaccac cagaacaaaa gtgatcaggg
     2161 ccatcttctc aaccttacga aagcgaaccc agctatagat aagcacaatc gccgtggcga
     2221 cgatcagcgc tgcagtagcc gcatagatgt cataaatctt gtaaaacgcg aaaaagacaa
     2281 ccagcggtaa aaaatcaaga aactgcttca ttttacgatt ccgtaatcaa atgcttaagg
     2341 ttattggcga atcaacatat acaggcggaa cagatagatg agcaatatgg cagaaatcag
     2401 gttgctcaag gtgttcgcca gtacggcacc aatttccggg gttaatgcgg caaaagaaga
     2461 ggcaaaaagc agcaacagtg tttttgccag caaccagctc agtactgcgg gtgccaccag
     2521 acgcatattc gcccaagtca gccgcatact gctacgcatc gaggcaaaaa tgcccatttt
     2581 gtcctgtacc agcatcaccg gtgccagggc cagtaaaatg gccatgataa ttcccggaac
     2641 gaccaccagc atgatgccaa tctgtactaa aagggtagtc agaaaaatca gaataaataa
     2701 cttgggcaat atcggcgcac tggcaccaat agctcgcagc gcactgactc tctgacccgc
     2761 agacaccagc tggataatta atattacgcc tccggcgaga atggcgttac cgattaattc
     2821 tgaaaaagtg gacgccgctg aagcctgcag caaaatttgt tgctgttccg gtgacatatt
     2881 ctgaaccagg tcgaacaacc cactactgcc gctaacgggc acgccgtcat tgagctgcgc
     2941 aagctgtgca tcactgggtg agaaaacatg ccctaacacc actgtgataa acgcacatag
     3001 caacgatacc aacagaatgg tcataaattg attacggaag aaatttccgg tgtcacggta
     3061 tacggactgc gccgtgatag acatgcactc tccttgagtt ttatgcaggt gttaattagc
     3121 gggcaattgt accctggtta agcctcgggt ggcagcatca ggtcttgtat ggaaaagcat
     3181 atctttgtaa agcggaggta attgctggcc ttcaggcgtg ggcaaaccgc ggcaagtggc
     3241 gatcgagtta atatttgcgt agagaaaata tttaaaaatt gatttaaatc acattaacca
     3301 ggattctcaa tgcaacttct aaattaatcc agatcaataa atggtgaatt atcatatgta
     3361 atgtgatcta tgtaggatca tttgttactc caatgtaggt atattcgtca cgtttttata
     3421 accataacga cggagcggat atgaaaaagt taacagtggc ggctttggca gtaacaactc
     3481 ttctttctgg cagtgccttt gcgcatgaag caggcgaatt ttttatgcgt gcaggttctg
     3541 caaccgtacg tccaacagaa ggtgctggtg gtacgttagg aagtctgggt ggattcagcg
     3601 tgaccaataa cactcaactg ggcttgacgt ttacttatat ggcgaccgac aacattggtg
     3661 tggaattact ggcagcgacg ccgttccgcc ataaaatcgg cacccgggcg accggcgata
     3721 ttgcaaccgt tcaccatctg ccgccaacac tgatggcgca gtggtatttt ggtgatgcca
     3781 gcagcaaatt ccgtccttac gttggggccg gtattaacta caccaccttc tttgataatg
     3841 gatttaacga tcatggcaaa gaggcggggc tttccgatct cagtctgaaa gattcctggg
     3901 gagctgccgg gcaggtgggg gttgattatc tgattaaccg tgactggttg gttaacatgt
     3961 cagtgtggta catggatatc gataccaccg ccaattataa gctaggcggt gcacagcaac
     4021 acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc agcaggatat cgtttttaat
     4081 tccgcacaaa aacgaccccg taatatacgg ggtcaataag gacatggtat aaagggtatt
     4141 atttcttcgc ttctacgcca tcagtttcag aacgaattaa aaatttctca gttgtttgcg
     4201 gaatatgctg aatcagccag tcggccattt gcttttcctc atttaaaatc gcttcgatga
     4261 tcggaattga gactgtatca ccggcatttt ttgctgctgc taatagtgag gtgtaacagg
     4321 tgatttcaaa ttgctcgaag acatatccgc caatagagcc tttaactatt tcatcagaag
     4381 ggaatatacc accgattgac tgcccaagcg cagccatttt actcatggaa tctttaatga
     4441 ctgaacgtga aatgtcatta cgatcaagaa tagtttccag ttgaacaatc tggtttttgg
     4501 tttcactaag atgttgttca atacgagcgc gtagttcagg ataattatct atacggctgg
     4561 ccatggattc aagcatagat tcggcttgct tttccattgc gtgggcgtca cgtaaccagt
     4621 catgataatg ttcaatacga ttcatgaaaa tgtcctcttt ataaaatgac taaaagttaa
     4681 aattcatatt tcaggcttta ttttcggctt tcttatttac gttattaagg gccagatcag
     4741 tcagtttgat gtcggtggcc ttttcttctt ccagggtttc tttcagaagc ttcgctgctt
     4801 tacggtaacc taattgttca gccagcgtcg ctaatgtccc gtaactggca atctcataat
     4861 gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg cacttcgttt ttctcggtac
     4921 tttcgatgac ctcattagct tcttcaataa gaccttccat tgccacacat ttcatgcgct
     4981 taattttcag atttgattct gattccacaa cttgatcaat tcgttcaatc tgtccatgag
     5041 tttcctcgag gtgcgcatga aaagcctgac ttaatttttc atttgatgtt gctcttgcga
     5101 gttttgccag tgcccgggtt aattgttttt ctgcgctgta ggtatctgaa agcagttgaa
     5161 taaatacatc ttcaatggtc ttcatattca tatttttctc cagtgaaatc actgcgagcc
     5221 atacggcccg cagatgcaat tatctttatc agctgatatt aataattaac ggtgaatcag
     5281 gatttgcctg atttattacc accgctttgt tgaccgcctt ttttacccgc ttcagatgcg
     5341 cgttgcggat catttttaaa attaccgccg ctatgctgac cgcctttacg gcctgcgtcg
     5401 gatgccttct cacggtcttc ggcgaaattt cctgaactac cacgatgttc ggccatgtta
     5461 tttctcccgt tgcgttgcat tgtttcatta atatgagtgt tgtgtgtcga cactcattaa
     5521 aattagtcgc taatgagaat tagtcaaatt aagcgcaacg agaagataga gggaaaatat
     5581 attttgagga acattctgga tatattaaca attacctgag gaatatgtga cttagagaaa
     5641 attattgatt ttactggtgt tatgttgcgg gattaatttg tttgattata ttactgttgg
     5701 gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc gaaacggtaa aaagataaat
     5761 attaaatgaa tttaggattt ttccggcttc attaaagaaa gttaaaatgc cgccagcgga
     5821 actggcggct gtgggattaa ctgcgcgtcg ccgctttcat cggttgtaca aaaactttca
     5881 gtgccgccag cattttctct ggctcattaa tatgttgctc gatgatttta acaatggccg
     5941 aaccagaaat cgcgcccgca gctcctgcat caatcgctgc ttttacctga tccggggcgg
     6001 aaataccaaa cccctgcaat ggaggtgcag cgttgtactc tttcagcttc gcaaccagat
     6061 gattgagggg taacgcggcg cggttttctg cgccggtcac gcccgctcgt gacagcaaat
     6121 atgtgtaacc acgaccgtaa gaggctatct ggcgcagcag gtcgtcatcg gcatttggcg
     6181 ggcagatgaa gataggtgcg acattatggc gcaacgcggc ctggcggaag ggcgcggact
     6241 cttcaactgg cacatcggca accagcaccg aatcgacgcc gactttctcg cactgggcat
     6301 aaaactcatc aatgcctttg ttaaacacca gattggcata catcaacagg ccaatgggaa
     6361 tggtcgggtg tttctggcga atcagtgcca gcatttcaaa acattgtgcc ggagtcacac
     6421 ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg gccatccgcc agtgggtcgg
     6481 agaaggggat acctaactcc agcgcgtcag caccggcttc aattagcgta tcgataattt
     6541 tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat gcgccttctt
     6601 tgcgctcctt caactgggca aacagagatt cgtagcgttc catcagattt cccctcgtgc
     6661 tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa ggttaaccac
     6721 cagtagctgc tctttatccg ggttttcgcg catcattttc agcgcatggg ccagggcgtg
     6781 ggaggattcc agcgccggga tgatcccttc gtgcaggcac agcgttttga aggcttcaag
     6841 ggcttcatca tcggtaatag acacgtaatc agcgcgtcca gtgctgttaa gatacgcgtg
     6901 ttgtggaccg acagacggga aatccagtcc ggcggagatg gagtaagatt cttcaatctg
     6961 cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa taaatgccca cgcgaccatg
     7021 ttttagcggt gcgccgtgct cgccagtttc gataccgtga ccaccaggct ccacaccaat
     7081 caggccgacg ttggtttcat tgatgaaatc agcaaacatg ccgatggcat tcgaaccgcc
     7141 gccaacacag gcgataacgg catccggcag gcgaccttct ctttccagaa tctgcgcttt
     7201 ggtttcttcg ccaatcatcc gctgaaactc acggacaatg gtcggataag gatgcgggcc
     7261 agccgcggtg cccagcatat agtgcgcggt ttcataactg ccggaccagt cgcgtagcgc
     7321 ctcattacag gcatctttca gcgtggcgga accgctatgt accgggatca cttccgcacc
     7381 cattaagcgc atacgaaaaa cgttaggcga ctggcgttca acgtctttgg cacccatata
     7441 aatacggcat ttcaggccga gcagggcgct ggcaagggcc gacgccacgc catgctgacc
     7501 ggccccggtt tcggcgatga tttcggtttt acccatccgc ttcgccagca acgcctgccc
     7561 cagcacctgg ttggttttat gtgcgccgcc gtgcagcaaa tcttcacgct tgagatacag
     7621 cgtggtgttc gtcccggctg taatgttctg gcatttggtc agtgcggttg gacgcccggc
     7681 atagtttttc agcaggtcgt tgaactgggc ttgaaattca ggatcttttt gcgcgctgac
     7741 aaaagcttct tccagctggc gcagagcagg catcaggatt tgtggcacgt acatgccgcc
     7801 aaactcacca aaataggggt taagtaatgt tgtcattgtt cctttcctta atatgcgcgc
     7861 agcgtctgga aaaccgaggc caaaagacgt gcgtctttga tgcccggttg cgactctaca
     7921 gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca gttatctgcg
     7981 cctaagcccc ccgccagcag aacgttgcca agcgattgac catttaatag tgaccagtca
     8041 aaacgttgcc cgcttccacc ctggccgttg tctaaaacat atttatcaac gtgctgaaac
     8101 tcgcgggcgg gcagggtttc accgacgctt aatgctttcc agatggcaac atgtgctggc
     8161 agagcttcac gcagcgtatc gatatacagc tgatcttcat taccatgcag ttgcactgcc
     8221 gccagcgata acaccttagc tttgtccacc acatcggcaa tatcgtgatt gcggaacacg
     8281 ccaacatact gcaacggtgc cgcagccatc acttcctgcg cctgttcaac gttgacgcaa
     8341 cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc ataagctgct
     8401 ttagcatctt gcccacgcgt caggccacat actttattct cacccagcaa cacccggcgc
     8461 acggcggcgt gcaaatcgtc atgggccatc aacgccgaac caatcagaaa accgttagcg
     8521 aagtggctta actcgcgcac ctgagcgtaa gtattgatgc cggattcgct gattaccgtt
     8581 acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat cgacaaatca
     8641 cgcagatcgc ggttgttgat gccaacgacc tttgccccta atgcaatggc gcgctccagt
     8701 tcctcttcat tactgacttc ggtcagcacc cccatctcca gactgtgagc gacggctgca
     8761 agctggcgat attggtcgtc atccagtact gaaagcatta ataagcaggc atcggcctgg
     8821 taatagcgcg ccagatagat ctggtaaggg tcgataatga agtctttaca taaaatcggc
     8881 tgcggggcga tttggctgac gatgaggagg aaatcaaagc tcccctgaaa atatttctca
     8941 tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa tggcggcaat gcgtgccgga
     9001 tcgaaatcat cacggatcac gccttttgac ggcgacgctt ttttacactc cagaataaac
     9061 gccgtgcgtg caccctgtag cgcatcataa aaatgtcgtg tgctcggctg aacctcattc
     9121 tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta cccaaatcgc cttgtctgcg
     9181 acgattttcg ctaaaacggt ttgcatcatt taccctcgtg ccgccagcgc ggtaactctg
     9241 tcgtaagcgg aaccactgcg cagtacctca agaacggttt gcgcattggc ttgcagatct
     9301 tcatggccat gcaggcgcat taacatggcg acgttcgcag cgacggctgc ttcatgggcg
     9361 gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac ggttttcttc aggtgttccg
     9421 cctgccagtt gctcctggtg gtagggagtc aggccaaaat cttcagcggt caattgatag
     9481 ctcttaattt tgccgtcatg cagctcggca acgattgtcg gcgcgtgtaa tgaaacttca
     9541 tccatcccgc cgctgtgcac caccgccgcg cgttgatacc ccagcacgcg caaggtttcg
     9601 gcaatcggca gcaccagttc cggactataa acaccaatta acgccagcgg cggatgcgcc
     9661 gggttaatca atggccccag cacattgaac agggtgcggg ttttcagttg ctggcgaacc
     9721 ggcatcgcgt ggcggaatcc ggtgtgatat ttcggcgcaa agaggaaaca tacgcctaac
     9781 tcgtccagcg cctggcgcga tttattggcg ttcatatcaa gattaatacc gaatgccgcc
     9841 agcagatccg acgagccgga tttactggag acgctgcggt taccgtgttt cgccactttc
     9901 agcccacagg ccgcggcgac aaacgcactg gcggtagaaa tattgatact gttgctgccg
     9961 tcaccgccag taccgacgat atcagcaaac agataatccg ggcgcgggaa cggcgctgcg
    10021 ttttccagta gcgcggttgc tgccccggcg atctcgttcg ggtgctcacc gcgaattttc
    10081 atgctcacca gcgccgccgc cagttgttcc ggcttcagct cgccacgcac caccgctgaa
    10141 aacagctggt ggctttcttg ttggctaagc gtctgcgcct gatacagttt ttccagaatc
    10201 ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc aggccagcgt ttgttccagc
    10261 aggcgagcgc cctgggtggt gagaatggat tccggatgga actggaatcc acaaacgcga
    10321 tccgcatcgt gacgtactgc catcaccatg ccattaaaat gggcgttgat ggttaaaccg
    10381 gccggaatgt tactgccaac cagcgagtga taacgcgcca ccggcagcgg gtttgttaat
    10441 ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg ctttaccgtg aagaatttcg
    10501 cccgcctgac cgacatagcc cccgtaagct tcgacaattg cctgatgtcc gaggcaaatg
    10561 ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt ccggcataca accggcttcg
    10621 ctcggcacac cggggccagg agaaagcatc agtaccggat tgctcatcgt cgccaggcgt
    10681 tcaattaagg tctgcgccgg aatatggttg cggtaaatca ccacgttatg accattgctg
    10741 cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat tatcgagcag cagaatgtca
    10801 gccatcagaa agtctcctgt gcatgatgcg cggtggcaat agcgcacagt acagcgcggg
    10861 ctttgttacg ggtttcgtcg gcttccgact gcggaacaga atcaaggact acaccagcac
    10921 ccgcttgcac ggtggcgata ccgttttcca ccagcgccga gcggatcaca atgcaggtgt
    10981 cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc gtagctgccg cggcgacgac
    11041 cttccgcctc ggcaattaac tgcatagcgc gtactttcgg cgcaccgctt aacgtcccca
    11101 tattcataca ggcgcgataa gcgtgcaggg cgtcaagatc gtgacgcagt tcgccgacta
    11161 cgcgagagac gaggtgcatc acataggaat aacggtcaac tttggtgaga tcggcgatgt
    11221 agcggctgcc aggggtgcaa atgcgtgcca gatcattacg ggcgagatca accagcatca
    11281 gatgttcaga cagctcttta tggtcagtac gcatttccag ttcaatacgg ctgtcgagat
    11341 ctctgtccag tgaaccatcg gcgcgacgac cgcgtgggcg tgttccggca atcgggtaga
    11401 tctcaatctg gcggctggtg gcatcatact tgagcgagct ttccggcgac gcgccaaata
    11461 gggtgaaatc attatcctgc ataaaaaaca tgtacgggct gggattactc tttttcagca
    11521 cgtaataggc cgccagcggt gacgggcagg gcagagagaa acggcgagat ggcaccacct
    11581 ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg cactacgcca ccgaactctt
    11641 catcgctctg attacattca caacgcatat gcggcacgga aaccactggc agcggcggcg
    11701 cggcttcggt cagttgctga cgtagttcgt tcaggcgagc agtgagacgt tgtttttctt
    11761 cttcattcgg agcaaacagg ctggcctgaa tacgggtgct ttttttctga tggtcaatca
    11821 ccatcagcgt ttcagcgaga taaaaacaga aatcagggca gttattttcc gctgacagtt
    11881 gcggtaaatc ttcaaatccc gccacaaggt cataagagaa caggccgccg aagaacattg
    11941 cttctcgttc ttccttcggt acattcaaca gattctgcaa taaacggaaa gcgtcaaaaa
    12001 ccgaaaggga gcataagcgg gcgtcttcat ccagcagtgg actgacaggg gggaagcgca
    12061 gcacacggca gtttggtgat tgttcacttt ccacacccgc aggcagggcg ttatccagta
    12121 gtgccagcag ggcttcgcca ttgccggaaa gtgcctgaat tgtgacggtg tcacctaatg
    12181 ctgtaatgcg cagcgcactg tctatcagca gcaggctttt taaatcatct ttgctgtcga
    12241 tatctgcgga ttccagcagc agcgttgccg gacgattccc acacaactgg tgaaaaagcg
    12301 cagtcgggtt gtcgcgataa gcgccttcgc aggttagcag ttcgagagtc ggtttttgtg
    12361 tttgcattgt tattctctaa ttttgttcaa aaaaaagccc gctcattagg cgggctgggt
    12421 atctgattgc tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct
    12481 ttcagtacga aaattgcttt cattgtcgat acccttttta cgtgaacttg cgcactagtt
    12541 aactggttcg atgattaatt gtcaacagct catttcagaa tatttgccag acccgttatg
    12601 atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc
    12661 agtgatttac gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc
    12721 attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac
    12781 cacagctgca atcgcgccag caagagagga aatttcacgt tcgggactgg cgctgaatct
    12841 tattcccggc gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct
    12901 gaatattgat attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg
    12961 caatcagcgg gcgcagcaaa ttgccgaaag acttgaaaaa gcgcaaatac ctggggcgct
    13021 ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg cgcggtcatt ttgcgcgttt
    13081 ccttgtggag tgcggcaaag ccagttcaat ggcagatgtc tttaaaaagt atctggcgcg
    13141 cgggaaaacc ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat
    13201 tcatcattct ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa
    13261 atggctgaaa agattggttg cgcattttgc cgaacaccac ggcgacgcga tggaagtcgc
    13321 gcagtgccag caatcgccca atgaacgcac ccagctggcc acacttgcgc gtcagcatca
    13381 tttatgggca tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg
    13441 taaactctgg ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa
    13501 caccacagag agggaattat gagccagttt ttttatattc atcctgataa cccgcagcaa
    13561 cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ctatccaact
    13621 gattccggct atgcgctcgg ctgtaaaatt gaagataaaa acgccatgga gcgtatttgt
    13681 cgtattcgcc agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgaa
    13741 ctctcgacct attcattcgt ggataacgtt gcgtttcgtt tgatgaaaaa caacacgccg
    13801 ggcaactata ccttcatcct gaaggggacg aaggaagtgc cacgccgtct gttgcaggag
    13861 aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gttgcttgaa
    13921 gcactgggcg aaccgatgct ttccacttcg ctaatgctgc caggcagcga atttaccgaa
    13981 tccgatcccg aagaaattaa agaccgtctg gaaaaacagg tagatttgat tattcacggc
    14041 ggctatctcg ggcagaaacc aacaacggtt attgatctta ccgatgatac gccggtcgtg
    14101 gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag tgcgtaactc tgtatactac
    14161 gcggccttaa aatggcgcta tacggcgcca ttgattctcc tgttcgacgc ctgtgaaggc
    14221 gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa gtgctggccc gcgctggcca
    14281 cggctcccgc cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa
    14341 aattgctaaa ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc gtatcgatgg
    14401 tcatctgatt tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa
    14461 accggaaggt gagttgtgta cccgtaacga tccggaaggg cgtccgacgg tgtttgaccg
    14521 tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg
    14581 tggtctgctg ctgttcacca ccgatggtga actggcaaac cgtttaatgc atccaagccg
    14641 tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg
    14701 tgatttgagt cgtggcgtgc aactggaaga tggtccggca gcttttaaaa ccatcaagtt
    14761 cagcggcggc gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg
    14821 tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga tccgtgttcg
    14881 ttacggtgat atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct
    14941 cgcccagact aactatctgc gcgaactggt ggagcttccg ccagaaacca gctctaaagt
    15001 cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg cggtgaaacg
    15061 tcacagtcag gtgagcggca gtcgtcgttc tggcgggcgt aataacggtt aatcaaagta
    15121 tgccggacgt catatccggc atttttacag attaataatc aatccctatt tgcgccttaa
    15181 caccggcatc aaacgcatgt ctgatggggc gcagctcact taccgtatcc gccagttcaa
    15241 gaatatcccg atgacagcct cgacccgtga taatcaccgt ctgttgatgt ggacgttcat
    15301 ttaacgcctg caccacttct tccagtggca aatagtcata cgccaccata tacgtcagtt
    15361 catcaagcaa aaccatatcc agtgaggaat cagcaagcat ccgctttgca tgttgccaga
    15421 cttcgcggca ggcggcggta tcagactcgc ggttttgtgt atcccaggta aagcccgttg
    15481 ccatcacctg aaactcaacg ccatgtggct ccagcagatt gcgttcgcca ttaggccagg
    15541 tgcctttaat aaactgcacg acgcccactt tttttccgtg accaactgcg cgtgttgccg
    15601 taccaaatgc cgcggtggtt ttgccttttc cattgccggt aaagacgatg ataataccgc
    15661 gttcatcctg ggcctgggcc acacgagcat ctactttttc tttcactcgc tgctgacgct
    15721 gttggtagcg ttcatcactc attgggaaat tcctggttta cggcccggtt gggcgtcaaa
    15781 ggtcatgccg gttttacggc ggctgtcatc gcccatcagc cagaggtaga gcggcatgat
    15841 atcagcgggt gttttaagtt tctgtggatc ttcggtcggg aaggcgcttg cgcgcattgc
    15901 ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat attcatcggc
    15961 cagtacctgc atcatccctt cggtggcaaa tttcgacgct gcatatgcac cccagttggc
    16021 tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt ccgatttgag
    16081 taataaagga agcagtgcct gggtgagcat aaaggtggca ttaacgttga cctgcatgac
    16141 gtcctgccag acctgcggat tttgttcgct cattgggcaa acatcgccga gcaatccggc
    16201 attatgcaaa acaccatcca gacgcggata attaacgaca atgcgctgtg ccagttgttg
    16261 gcaattttcg gacgtgcagg tcagcaaatc gagaataaac cactgtggct gacgcccagt
    16321 ttcttcgttt atgtggctgg ctacctgacg taatttttct tcattacggc ccaacagaat
    16381 cactgtcgca ccatagcgtg catacgtcat cgcggcttca cgaccaatcc catcgctggc
    16441 tcccgtcacc aggataatgc gatcattgag taaatcttgt tttggctggt aatgcatggc
    16501 tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt ggggctttat
    16561 gcctgaaaca gaagggcatt tcaatcagcc tggggcgata acaccgcaaa atggatactt
    16621 tgtcatactt tcgctgcaat aacatctctg cgagacggct taacatgcct gttgtaaact
    16681 gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg aattgttgtc tgaatatggt
    16741 ttgtttttgg ctaaaatcgt taccgttgtg ctagcgattg cggcgattgc cgccattatt
    16801 gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa caatctcagc
    16861 gaacagtata aggagatgaa agaagaactg gccgcggcgc tgatggactc acatcagcaa
    16921 aaacagtggc acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc agcaaaagcg
    16981 aaagccaaac tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt gctggatttt
    17041 aaaggcagca tggacgccca tgaagtgaac tcgctacgtg aagagataac ggcggtactc
    17101 gcagcattca aaccgcagga tcaggttgtg ctccgtctgg aaagccctgg tggcatggtg
    17161 catggttacg ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa cattccttta
    17221 actgttacgg tagacaaagt cgctgccagc ggcggttaca tgatggcctg tgtggcggac
    17281 aaaattgttt ccgcaccgtt tgctattgtg ggttccattg gggtggtggc gcaaatgccc
    17341 aactttaacc gcttcctgaa aagcaaagat attgatatcg aactgcacac cgccgggcag
    17401 tataagcgca cgctgacctt gctgggtgaa aataccgaag aagggcggga gaaattccgc
    17461 gaagagctga acgaaacgca tcagttattt aaagattttg tgaagcgtat gcgtccgtct
    17521 ctggatattg aacaggtggc aacgggtgaa cactggtacg gacaacaggc ggtagagaaa
    17581 ggcctggttg atgaaatcaa caccagtgat gaagttattc ttagcctgat ggaaggccgt
    17641 gaagtggtta atgtacgcta tatgcagcgt aaacgactca ttgaccgatt caccggcagc
    17701 gcggcagaga gcgccgatcg attgttgcta cgctggtggc agcggggtca aaagccattg
    17761 atgtaaaaga caaacgcgag gctaagacct cgcgttttgc tttaatcaac cagatgatat
    17821 ttttctgaaa gcacatgggc caggtgtttg aacatattaa acaccgcggt gcttttggct
    17881 gttggcaatc cttgttcatc taaaaagtag tcgccggtaa ataccagcac gccattacgc
    17941 tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct catgctcacg aatgattttg
    18001 ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt cattatttac
    18061 tccttaaaca aggacattag tctacgccag gcatggcttg cagacaaata taccacgctg
    18121 gtggcaagag cgccttactg gcaactttgg attttgcatg ctaataaagt tgcgtatcgg
    18181 attttatcag gtacagtgtg acgctttcgt caatctggca atagatttgc ttgacattcg
    18241 accaaaattc cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc agtcacctga
    18301 attttcgtga acagagtcac gacaaggggt tgatatccgc agagagcgag tccatatcgg
    18361 taactcgttg ccagtggaag gtttatcaac gtgcgacgca ttcctggaag aatcaaatta
    18421 ggtaaggtga atatgggtaa agctcttgtc atcgttgagt ccccggcaaa agccaaaacg
    18481 atcaacaagt atctgggttg tgactacgtg gtgaaatcca gcgtcggtca catccgcgat
    18541 ttgccgacca gtggctcagc tgccaaaaag agtgccgact ctacctccac caagacggct
    18601 aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg
    18661 cacaattggg aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg
    18721 aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg
    18781 gaagccattg catggcacct gcgggaagtg attggtggtg atgatgcgcg ctatagccga
    18841 gtggtgttta acgaaattac taaaaacgcg atccgccagg catttaacaa accgggtgag
    18901 ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg cgtggtgggg
    18961 tatatggttt cgccgctgct gtggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg
    19021 cagtcggtgg cggtccgcct ggtggtagag cgtgagcgtg aaattaaagc gttcgtaccg
    19081 gaagagttct gggaagtcga tgccagcacg actacgccat ctggtgaagc gttggcgttg
    19141 caggtgactc atcagaatga caaaccgttc cgtccggtca acaaagaaca aactcaggct
    19201 gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga caaaccgaca
    19261 accagtaaac ctggcgctcc ttttattacc tctacgctgc aacaagctgc cagcacccgt
    19321 cttggttttg gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga agcaggctat
    19381 atcacttaca tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa tatggttcgc
    19441 ggttatatca gcgataattt tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc
    19501 agcaaagaaa actcacagga agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg
    19561 gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt
    19621 cagttcgttg cctgccagat gaccccagcg aaatatgact ccacgacgct gaccgttggt
    19681 gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc gctttgatgg ctggacaaaa
    19741 gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct taccggcagt cgataaaggc
    19801 gatgctctga cgctcgttga actaacacca gcccagcact ttaccaagcc gccagcccgt
    19861 ttcagtgaag catcgctggt taaagaactg gaaaaacgtg gtatcggtcg tccgtctacc
    19921 tatgcgtcga tcatttcgac cattcaggat cgtggctatg tgcgagtaga aaatcgtcgt
    19981 ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag
    20041 ttaatgaact acgactttac cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac
    20101 gaagcagagt ggaaagctgt actggatcac ttcttctcgg atttcaccca gcagttagat
    20161 aaagctgaaa aagatccgga agagggtggt atgcgcccga accagatggt tctgaccagc
    20221 attgactgcc cgacttgtgg tcgcaaaatg gggattcgca cagcgagtac cggggtattc
    20281 cttggctgtt ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac cattaacctg
    20341 gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag atgctgaaac caacgcgctg
    20401 cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg acagctatct catcgatccg
    20461 aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg acggttacga gatcgaagag
    20521 ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct
    20581 gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt
    20641 aaaaacacac gtaagatttt acgtaacggc gaagtggcac caccgaaaga agatccggtg
    20701 ccattacctg agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg tgacggtgct
    20761 gccggtgtgt tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg tgcgccactg
    20821 gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat
    20881 gcgccacagc aggatccgga aggtaataag accatggttc gctttagccg taaaaccaaa
    20941 cagcaatatg tctcttcgga aaaagacgga aaggcgactg gctggtcagc attttatgtt
    21001 gatggcaaat gggttgaagg aaaaaaataa cctttaattc tgtcaggttt ttataaacaa
    21061 agggtcgcga aagcggccct tttttatt
//
LOCUS       DAECOD010000067        21084 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.067, whole genome shotgun sequence.
ACCESSION   DAECOD010000067 DAECOD010000000
VERSION     DAECOD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.067"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..60)
                     /locus_tag="KKO15_002541"
     CDS             complement(<1..60)
                     /locus_tag="KKO15_002541"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361292.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KKO15_002542"
     CDS             171..1661
                     /locus_tag="KKO15_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD6361293.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KKO15_002543"
     CDS             1713..2870
                     /locus_tag="KKO15_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD6361294.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KKO15_002544"
     CDS             complement(2836..2958)
                     /locus_tag="KKO15_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361295.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="KKO15_002545"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="KKO15_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="KKO15_002546"
     CDS             4884..7244
                     /locus_tag="KKO15_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD6361296.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..10084
                     /locus_tag="KKO15_002547"
     CDS             7301..10084
                     /locus_tag="KKO15_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD6361297.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSHLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10445..11845
                     /locus_tag="KKO15_002548"
     CDS             10445..11845
                     /locus_tag="KKO15_002548"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6361298.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12001..13536
                     /gene="gadC"
                     /locus_tag="KKO15_002549"
     CDS             12001..13536
                     /gene="gadC"
                     /locus_tag="KKO15_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBD6361299.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13667..14986
                     /locus_tag="KKO15_002550"
     CDS             13667..14986
                     /locus_tag="KKO15_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBD6361300.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15284..15325
                     /locus_tag="KKO15_002551"
     CDS             15284..15325
                     /locus_tag="KKO15_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361301.1"
                     /translation="MVQLVDLARCVSF"
     gene            15363..16498
                     /gene="dosC"
                     /locus_tag="KKO15_002552"
                     /pseudo
     CDS             15363..16498
                     /gene="dosC"
                     /locus_tag="KKO15_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            16423..17040
                     /locus_tag="KKO15_002553"
                     /pseudo
     CDS             16423..17040
                     /locus_tag="KKO15_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17298..17630
                     /gene="ddpX"
                     /locus_tag="KKO15_002554"
                     /pseudo
     CDS             17298..17630
                     /gene="ddpX"
                     /locus_tag="KKO15_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17644..18540
                     /locus_tag="KKO15_002555"
     CDS             17644..18540
                     /locus_tag="KKO15_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBD6361302.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18537..19523
                     /locus_tag="KKO15_002556"
     CDS             18537..19523
                     /locus_tag="KKO15_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6361303.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19516..20442
                     /locus_tag="KKO15_002557"
                     /pseudo
     CDS             19516..20442
                     /locus_tag="KKO15_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20498..20929)
                     /gene="osmC"
                     /locus_tag="KKO15_002558"
     CDS             complement(20498..20929)
                     /gene="osmC"
                     /locus_tag="KKO15_002558"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBD6361304.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5678 a         4936 c         4955 g         5515 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagcgcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggacgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcacct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
    10081 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10141 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10201 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10261 acaccttgcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10321 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10381 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10441 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10501 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10561 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10621 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10681 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10741 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10801 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10861 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10921 cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga
    10981 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
    11041 cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
    11101 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
    11161 catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt
    11221 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
    11281 ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact
    11341 ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg
    11401 cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta
    11461 taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa
    11521 actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg
    11581 cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
    11641 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
    11701 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
    11761 agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca
    11821 gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc
    11881 cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg
    11941 aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat
    12001 atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc
    12061 ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt
    12121 tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt
    12181 gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca
    12241 aatactctgg gtccgagatg gggatttgca gcgatctcat ttggctatct gcaaatcgcc
    12301 attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg
    12361 ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat tctttgggcg
    12421 ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc
    12481 ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttacctgcac
    12541 tccggtgccc ccgttgctat cgaaatggat tcgaagacct tcttccctga cttctctaaa
    12601 gtgggcacac tggttgtatt tgttgccttc attttgagtt atatgggcgt agaagcttcc
    12661 gcaactcacg tcaatgaaat gagcaacccc gggcgcgact atccactggc tatgttactg
    12721 ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc gatggtcatt
    12781 ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc
    12841 catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt
    12901 gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg
    12961 cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg
    13021 ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt
    13081 ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt
    13141 gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa
    13201 cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg
    13261 ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgat
    13321 tctaccgata tgtatgttga attactggtt gttagtttcc tggtggtact tgccctgccc
    13381 tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt cactctggag
    13441 ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca
    13501 ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagaca
    13561 agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga
    13621 taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc
    13681 ccgaaacaag aaattaacga ttagaagacc agcgatactg gttgcattgg cacttttact
    13741 gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag gttcaaagcc
    13801 accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtttc
    13861 tcgcctcgac tggccaccgg tttcctcggt taacattagt aatcccacca gccgagcccg
    13921 tgtacaacaa caggcgatga tcgacaaact ggatcatctc caacgtctcg gcataaacac
    13981 ggtctttttc caggtcaagc cggacggtac cgccctgtgg ccatcgaaaa ttttgccgtg
    14041 gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc tgcaattcat
    14101 gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt
    14161 atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt ctcaacaacc
    14221 ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc gctttgtcct
    14281 cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgctg aagtggtttc
    14341 ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgaat caccgggttc
    14401 acggctaaat gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga
    14461 ctggcggcgc aacaatactc agcagttaat tgcaaaggta tcacacacca ttaaaagcat
    14521 taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga
    14581 tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg cagacacccg
    14641 tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc
    14701 acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg ttaaaccaac
    14761 caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga
    14821 gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg atcttaacga
    14881 tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga ataaaccgca
    14941 gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaacta ccatgcttac
    15001 tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat atcccactag
    15061 taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt taaacagcta
    15121 taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa
    15181 aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcttaa
    15241 gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga
    15301 tctcgcacgt tgcgtttcgt tttgattccg tctgatttcc cagccttata agggtgtgaa
    15361 ttatggagat gtattttaaa agaatgaaag atgagtggac cggacttgtc gaacaggcag
    15421 atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat tatctgagta
    15481 tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga agaattcttg agtaatgaac
    15541 aagttgagcg gcagttgaaa agtgcgatgg aacgctggat tattaacgtg ctttctgccc
    15601 aggttgacga tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa gtgcatgccc
    15661 gcataggaat tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa aaaatcctct
    15721 atccggtaat cttctcttcg gattattccg ccgcagaaaa acttcaggtc taccatttct
    15781 cgattaacag tattgatatc gcgatggaag tgatgacccg cgcgtttacc tttagtgaca
    15841 gtagtgcctc aaaggaagat gaaaactatc gtatcttctc gttactggaa aacgccgaag
    15901 aagaaaaaga acggcaaata gcctcaatac tttcatggga aatagatatt atctataaag
    15961 tcctgctgga ttctgattta ggcagcagtt tgcctttaag ccaggctgat tttggcctgt
    16021 ggtttaacga cattatttta gcggcattgc cgaagtgggc catatctccc gtctgattca
    16081 ggatttcgac ggtattttca atcaaaccat gcgtaacacc aggattttga ataacagaag
    16141 tttgcgggtg aaatttctat tacagataag aaataccgta tcgcagatta ttaccttgct
    16201 gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt atggatgtac tgacgaaatt
    16261 acttaaccgc cgtttcctgc cgactatctt caaacgcgaa attgcccatg ccaaccggac
    16321 cggtacaccg ctgtcagtgc tgattattga cgttgataaa ttcaaagaga tcaacgatac
    16381 gtggggccat aacactggcg atgaaattct gcgtaaagtc tcattcctct cgcagaagag
    16441 attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca
    16501 gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac
    16561 tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg gggtattgac
    16621 ggccaccagc tgacggtgga aatcacggaa agcatgatga tggaacacga taccgaaatc
    16681 tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt tatcggtaga tgattttggc
    16741 acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat caaaattgac
    16801 aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc tggccttact tgaagccatt
    16861 accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga aaccaaagag
    16921 caatttgaga tgctacgtaa gatccactgt cgcgttattc agggatattt cttttcccgc
    16981 ccccttcccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct gaaaatctga
    17041 caaattcctc tcgcccgcac tcgcgggttt tcctttaagg tgacactgtc acttaaatac
    17101 tgtgatttca gctacgattc cgggagattc cttccttaac tttccctctt gcaccaaaac
    17161 tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa gccagcattc
    17221 tggcgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc aatttttgtc
    17281 ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc gtgatcttcc
    17341 ctgatctgga gatcgaattg aaatatgcct gcgttgataa catcacaggt aaagctattt
    17401 atcagcaagc gcgttgtctg ctacataaag atgcgattac cgcgctggcg aaaagtatca
    17461 gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc ccacaacaag
    17521 cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat gtgacggtcg
    17581 gttctaatca cagccgtggc acggcgctcg acctgacgct tcgtgatgac gtggaggtgg
    17641 tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac
    17701 ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc
    17761 attgtattaa tgcgactgat gatgattttt tcaccgtggc tggcgacgca tgatcccaac
    17821 gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat
    17881 gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc aattctcgcc
    17941 ggattagtgg tggtcgccat tgcgggtatg attggttcgc tactcggatg tctatccggt
    18001 gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca tggacattat gctgtcgatt
    18061 ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt atttaacgcc
    18121 atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcttggcgcg ggggcaagca
    18181 ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggcgc gtctcgttgg
    18241 catctgatca actggcatat tttacgtaac tccctaccgc cgctgatcgt gcaggcatcg
    18301 ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctaggtgct
    18361 caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat
    18421 cagtggtggt attgcgcatt tccgggggca gcgattttgc ttacctccgt cgggtttaat
    18481 ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga
    18541 cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt aacggtgatg
    18601 ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc ggtctggtgg
    18661 gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg
    18721 gcagttattg cgtacatcgg gggcagattt cactgctggg agaagatgtt cttaacgctc
    18781 gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga
    18841 tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgta atccgccatc
    18901 atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg gaagagatgc
    18961 aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc
    19021 gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccggcg
    19081 aaccgacgac ggcgctggac gtcacagtac agttgcaggt actgcgtttg cttaaacata
    19141 aagcacgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc
    19201 aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgggacgg
    19261 cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa tgcgcaccag
    19321 aacatggagt accacgccag ccattacccg ccattccagg gacggtacca aacctcaccc
    19381 atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa
    19441 acgtcccggc gctgacagcg tgtggtgaca acaaccagca ctgcgcctgt tggtatccgc
    19501 agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttt
    19561 ccggcccgta aaaactggct tggtaaatct acggaacatg ttcatgccat taatggtatt
    19621 gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa
    19681 agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca gtacatccgt
    19741 tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc gcttaatccg
    19801 cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg tagtagtgaa
    19861 caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg tccggagtat
    19921 ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccaga
    19981 gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc gctggatatc
    20041 tccgtgcagg cgcagatcct caatttgctg gtaacgctac aggaaaatca cgggctgact
    20101 tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg ggtggcggtg
    20161 atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac tgcttcggca
    20221 catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc gctggaggaa
    20281 gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca aggctgtttt
    20341 ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc attagcaata
    20401 agggaggacg gacgtgagct ccggtgctgg cgggcgctgt agagatgcta acaacgcatc
    20461 aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac tggtaatcca
    20521 gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct ttctggatga
    20581 tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc agtgcgattt
    20641 tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg gtatcaattg
    20701 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
    20761 catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
    20821 taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt cccttcccgc
    20881 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
    20941 tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata
    21001 ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc ccacaataga
    21061 taaccgggaa tatatatctc aaga
//
LOCUS       DAECOD010000068        20892 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.068, whole genome shotgun sequence.
ACCESSION   DAECOD010000068 DAECOD010000000
VERSION     DAECOD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.068"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="KKO15_002559"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="KKO15_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBD6361305.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="KKO15_002560"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="KKO15_002560"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBD6361306.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="KKO15_002561"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="KKO15_002561"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBD6361307.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="KKO15_002562"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="KKO15_002562"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBD6361308.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="KKO15_002563"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="KKO15_002563"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD6361309.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="KKO15_002564"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="KKO15_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBD6361310.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="KKO15_002565"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="KKO15_002565"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBD6361311.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="KKO15_002566"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="KKO15_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBD6361312.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="KKO15_002567"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="KKO15_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBD6361313.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="KKO15_002568"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="KKO15_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBD6361314.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLLLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11403)
                     /gene="rsxC"
                     /locus_tag="KKO15_002569"
     CDS             complement(9181..11403)
                     /gene="rsxC"
                     /locus_tag="KKO15_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBD6361315.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11396..11974)
                     /gene="rsxB"
                     /locus_tag="KKO15_002570"
     CDS             complement(11396..11974)
                     /gene="rsxB"
                     /locus_tag="KKO15_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBD6361316.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11974..12555)
                     /gene="rsxA"
                     /locus_tag="KKO15_002571"
     CDS             complement(11974..12555)
                     /gene="rsxA"
                     /locus_tag="KKO15_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBD6361317.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12632..13072)
                     /locus_tag="KKO15_002572"
     CDS             complement(12632..13072)
                     /locus_tag="KKO15_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBD6361318.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13158..13373)
                     /gene="ydgT"
                     /locus_tag="KKO15_002573"
     CDS             complement(13158..13373)
                     /gene="ydgT"
                     /locus_tag="KKO15_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBD6361319.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13646..13771)
                     /gene="blr"
                     /locus_tag="KKO15_002574"
     CDS             complement(13646..13771)
                     /gene="blr"
                     /locus_tag="KKO15_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBD6361320.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14014..15054
                     /locus_tag="KKO15_002575"
     CDS             14014..15054
                     /locus_tag="KKO15_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6361321.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15090..16091)
                     /gene="add"
                     /locus_tag="KKO15_002576"
     CDS             complement(15090..16091)
                     /gene="add"
                     /locus_tag="KKO15_002576"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBD6361322.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16195..17367)
                     /gene="malY"
                     /locus_tag="KKO15_002577"
     CDS             complement(16195..17367)
                     /gene="malY"
                     /locus_tag="KKO15_002577"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBD6361323.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17377..18969)
                     /gene="malX"
                     /locus_tag="KKO15_002578"
     CDS             complement(17377..18969)
                     /gene="malX"
                     /locus_tag="KKO15_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBD6361324.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19144..20172
                     /gene="malI"
                     /locus_tag="KKO15_002579"
     CDS             19144..20172
                     /gene="malI"
                     /locus_tag="KKO15_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBD6361325.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20284..>20892
                     /gene="hdhA"
                     /locus_tag="KKO15_002580"
     CDS             20284..>20892
                     /gene="hdhA"
                     /locus_tag="KKO15_002580"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBD6361326.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PSDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5322 a         5259 c         5362 g         4949 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgatg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttga
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgctggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag agtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gccgcttcga cggcagcttt gcgcggatca acctgttctt ctggctccgc
     9481 attggcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg cttcgacggc
     9541 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9601 gcgcgctttg gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgacctgttc
     9661 ttctggttcc gcattagcct gttgctgttc cagcttgcgc gctttggagc gggcgatagc
     9721 tgcttcaacg gcagttttac gtggttcagc aacggttgct gcgtcgttag tttgctccag
     9781 ttctgcctgt ttcgctctgg cttgcgcttt acgggcttcc cgttctgcaa taattgcact
     9841 gttatccggg cgttcgcccg ctttaatcac aataggctgt gtagcctggg cctgtttctc
     9901 tttcacccgc gccagagcgg cagcaatcgc atctttatct ttggctgcag gttgaacggc
     9961 tgcgctctta tgtcgttcaa ggcgagtcgc tttttcgcgc tccagacgag cctggcgcgc
    10021 ttcgaaacgc gctttggctt ctgcggcgcg cttttcttcc tgacgaatag ccgcaatttc
    10081 agctttttcc tgacggaaat attgcaccaa gggaatattg ctcgggcaaa cccacgcgca
    10141 agccccacat tcaatgcagt cggcaatgtt atgcgtggta gctttatcgt gttgctgacc
    10201 tttgctgaac cagtacaact gttgcggcaa aagatccaca gggcaggcgt cagcacaggc
    10261 gctacaccgg atgcagcttt gttcttcctg tggttcgcca agttcattgg cagagggagc
    10321 caacagacag ttggtaatct ttacgaccgg gacatccagc catggcaagg taaagcccat
    10381 tagcgggcca cccataatca ccatttgatc ggcagagggg cagaatccgg catcattcaa
    10441 taaatgacgc actggcgtcc ccagccgtgc ccagacgttg cccgggcgag cgattgcttc
    10501 gccagtcagg gttacaacac gctcggtaat cggctcgcca tcaataacgg cacgtttcac
    10561 tgcataagca gtgccgacgt tttgcattaa tacgccgata tcggatgaac gcccgccatg
    10621 tggaacctgc ttcccggtca gaatgtaggt taattgttta gcaccgccag aaggatattt
    10681 ggttggaatc acccgcagag aaatatcgtt agagtccgcc agcaccgcgc gcagcatgga
    10741 aatcgcctgc ggtttgttat cttcaatgcc gataagaatt tcgcgtggct gcagaatatg
    10801 cgcaagaatg cgaataccct ctacgacctg agccgcgcaa tcctgcatca aacggtcatc
    10861 ggcggtaatg tacggctcgc actcagcagc gttgataatc aacgtttcaa tcttatctcc
    10921 accaccctgc aatttaacgc ctgtcgggaa tccggcaccg cccagcccgg caacaccaaa
    10981 ctgatgtatg cgctcgatta actcttcgcg actgcgagag cgataatcga cccagccgtc
    11041 gcgcgggatc cagcagtctt caccatcggc atcaataatc acgcttaatt cagctaaagc
    11101 tgaaggatga gccgtagagt ggggcgcaat agccgtaacg gtacccgagg tgggcgcgtg
    11161 aacaggcagc attttgccgc gaccacgggt aagcggctgg ccgcgcaata ctttatcgcc
    11221 gacgctaacg cacaactcac cttcagcgcc aatatgctgt ttcagtggaa taacaaaatg
    11281 ctgcgccagt agtacctggc gcaggggggt accgttggtc tgggttttca tctccggtgg
    11341 atggatgccg ccgttgaaat cccagatttt attttttctg aatgcagaga ataacttaag
    11401 catggtgttc cacgggaatg atacgcacgg gaatggtgtt cagatcccat ttccaggagt
    11461 caggtgtttc tgcgaccggt tgcaacgaga tgcagtgcgt cgggcacgga tcaacacata
    11521 aattgcagcc cgtacagaga tcactcatta ccgtatgcat gacacgggta gcgccaacga
    11581 tggcgtctac cggacaagcc tgaatacatt tagtgcagcc aatacagtta ttttcatcaa
    11641 taaccgccac catccgcgca ggcgttagct cttgcgcttc gccatccagc ggctgcggct
    11701 cgacattaag caactcggca atttttagca tcacagcttc gccacctggg gcgcaacggt
    11761 tgattttttc accgttacag ctgatggctt ccgcgtaggg gcgacagccg ggataaccgc
    11821 actgaccaca ctggctctgc ggtaagattt cgtcaatttt ctcaacgacc ggatcgtctt
    11881 ccaccgcaaa acggcgggag gcataaccca gaatggcgcc aaacgccagg cccagcaggc
    11941 tcacggcggc aacggcaatc cagatagcat tcattacaac ttcaccaaac cactaaagcc
    12001 cataaaggcc agagacataa gacctgcggt aattaacgca atggcattac cgcgaaaagg
    12061 tgccgggaca tcagccacag caaggcgttc gcggatggcg gcgaagagca ccatcaccag
    12121 cgagaaaccg acagcggcgg aaaaaccgta cagcgccgac tgcaagaaat tgtgcccgag
    12181 attgatattc agcaacgcca cgccgagcac tgcacagttg gtggtgataa gcggcaaaaa
    12241 aatccccagc agacggtaaa gcaccgggct ggttttgcgc accaccatct cggtgaactg
    12301 cacgaccaca gcaatcacca gaataaatgc cagggtgcgc aggtaaatca gattaagtgg
    12361 gatcaaaatc cacgtatcga taagccaggc gcaaatagac gtcagcgtca tcacaaacgt
    12421 tgttgccagc cccatgccca tcgcggtttc cagttttttg gaaaccccca taaacggaca
    12481 gagaccgaga aacttgacca gtacaaagtt attgaccagt acagttccga caaacagtaa
    12541 caggtagtca gtcatttttc ggcctgaaat aaaaaagccg cctattatcc gttaatccag
    12601 agcaggcgac aacaggttaa ctgtaaggtt attacggatt cacaaacgtc gcttttaccc
    12661 gcgacgagcg tttgaaatag gggacgatca atgcggttgc caacaaagta aacagcaact
    12721 ggcgaacggc tatgccaact tccacgggtg aaaaagcgaa tgcttttact gccagcaata
    12781 cggaaataag cagccagata atatagtgtt taggaacaca acgccgacgt ttaaagaaag
    12841 cgatggtcaa ccacagtgtg taataccaca tggcgatagc ggtaataaaa gaaacgcccc
    12901 ataagatttg tgtcgtcagt gcttgtccgc cgagcgtttg aaatgtttgc ggagaagata
    12961 acgcagcggt atacagcaac aacgccagcg tcgtactcaa cagagcgact aaaagccagg
    13021 ccaacgggcc aagcaaccag cctccaatac gttgtggcgt tgtggtggtc ataggttctc
    13081 cagaatagcg acgcaaacag gcaaatctaa ttttaggggc gttattataa ggcttttata
    13141 accgatcgct actttcttta ttggacatag tgccagacgg acttcggtac ctggccgagg
    13201 tcaaacaaac gcccgccaga aaccagctct gcgcgacggt gatcggcagc acgatacata
    13261 ttgatcagtt cctgatcgtc cgtcagggtg taattaagat ggtcgtagag tttttccaga
    13321 ctttcgagtg aactgatttt gcgaaatttt aataagtagt cctgaacagt cataaaaggt
    13381 ccataaaata attaatacca aaggggtagg tattattagt gatgataaaa tttcaccaaa
    13441 agtgaacgct tgtgttatta caataataag aatgcgctga tggcaaatat tcagggatga
    13501 aaaactgctc tgaaaagagt aacgcgcttt actatccctg acaatcacca acaacatcga
    13561 acaagataat aaattcctgg tttaatatcc gacaagtgaa aacatgcacc cggacgggca
    13621 gcatgtcgct ccacaagtgc agagcttact tgtgttgtac cgaagcactc tgttcaggtg
    13681 gctgatagtt gtcaatgtga ctcgccacgc caagaagaat gactgaaacg acaagaacga
    13741 tccaacctgt taattcaata agacgattca ttacagccca catctctctt gattgatcca
    13801 ttaacttcag ggggtaaatg ttacttagca ataatagctc cagcaagatt tttactgagg
    13861 tttttgcgat atttagcttt tgtcgttgga aaattcgcta tttttttgac ttaagttaaa
    13921 caacatccct tattgctggc aggttattaa actgttgagc gtgggtaaag gatagtgtca
    13981 aatagccatc atacttcaat gagaggcaat gacatgagcg acaacatccg tgttgggttg
    14041 attgggtatg gttatgcgag caaaaccttc catgcgcccc tgattgcggg cacgcccggg
    14101 ctggaactgg cggtaatctc cagcagtgat gaaacaaaag taaaagccga ctggccaacg
    14161 gttacggttg tctctgagcc gaagcatctg tttaacgatc ccaacataga cctgattgtc
    14221 attcctacac ccaacgatac ccatttcccg ttagccaaag cggcgcttga ggcgggtaaa
    14281 tatgtggtcg ttgataaacc ctttaccgtg acactgtcac aagcgcgaga gctggatgcg
    14341 ctggcaaaaa gcctggggcg tgtgctgtct gtattccata accgtcgttg ggatagcgat
    14401 ttcttgacgc taaaaggttt gctcgcggaa ggcgtgctgg gtgaagttgc ttactttgag
    14461 tctcattttg accgcttccg tccgcaggtg cgcgatcgtt ggcgtgaaca gggcggccca
    14521 ggcagcggta tctggtacga tttagcaccg catcttcttg atcaggccat tacgctattt
    14581 ggtttaccgg tcagcatgac ggtagatttg gcacagttac ggcccggagc gcagtcgacc
    14641 gattatttcc acgccatctt gtcctatcca cagcggcgag tcattttaca cggtaccatg
    14701 ctggcagccg ctgagtcagc acggtatatc gtgcatggat cccgaggcag ttatgtgaaa
    14761 tatggcctcg atccacagga agaacgtctg aaaaatggcg agcgtctgcc gcaggaagac
    14821 tggggctacg atatgcgtga tggcgtactt acccgcgtgg aaggtgagga acgtgtcgaa
    14881 gaaacgctgt tgacggtgcc tgggaattat ccggcttact atgcggcgat tcgtgatgcg
    14941 ttaaatggcg atggtgaaaa tccggttccg gcaagccagg caatccaggt aatggagttg
    15001 attgagctgg gcatcgaatc cgccaaacat cgcgcgacgt tgtgccttgc gtgaaaaatg
    15061 gatggtgcaa tttgcaccat ccatttttgt tacttcgcgg cgactttttc tcgcagtgcg
    15121 cgtttttcct ctgcgctgag gaaagccatt tccagaccat taatctgtgc ctggcggatt
    15181 tgctcacggg ataacccagc agctggcgcg gcaacggtat attcgtgaat gatatccact
    15241 ccctgtacgc cgggatcgtc agtgttaatg ctggcacgaa tgccatgctc aaggaacgtt
    15301 ttcagcggat gtgcagccag ctctgctacg gtgctggtct gaatattgga ggtcagacag
    15361 gattcaatac caatttgttg ctcggcgaga aaatccatca gcgcccgatc ttcaatggct
    15421 tttacgccat gtccaatacg ctccgcaccc agttcacgaa tcgcctgcca gatgctttcc
    15481 ggcccggcag cttcgcctgc atggacggta atatgccagc ccgcatcacg cgcgcggttg
    15541 aagtgagaaa ggaacagact tcccgggaat ccaagttcat caccagctaa atcaagtgcg
    15601 gtaatctggt cacggtgggc taaaaaggcc tccagctctt gctgacaggc ggcttcgccg
    15661 aaggttcggc tcataatgcc gataagcttc gcctgcacac caaaggtgcg gcaaccttca
    15721 cgtacgccat cgatcaccgc ttcgacaaca cccgctacag gcagcttatg tgccattgcc
    15781 atgtagcctg gtgaaaaacg cagctcgaca tagcgcaggc cgttacgggc tgcatcttca
    15841 atgttttcaa atgccacgcg gcggcaggca tcaagagagg cgagaacttt aacgccccag
    15901 tcaagtttgg tcagaaagct caccagatcg ggttcgttgg caatgacctg aacgtgggga
    15961 atcagtgttt caagggattg tgcaggaagc gagatattat actggcggcc aagttcaaga
    16021 atggtctggg gacgaatgtt gccatcaagg tggcgatgga tatcagttaa tggcagggtg
    16081 gtatcaatca tggtcgcact ctttttctgg atataaaagt gcgcaccatt ataaaaacaa
    16141 agcagggtaa aaatccacga ttgcgcaacg attttttacg ttgcgcaatt ggggttagcg
    16201 aacagcgcgg atggcgttta ttaatccagc cacacctttt tccagtttcg aacgtgggca
    16261 gccggcattg agacggacaa aaccacgacc ttcttcacca taggtatacc ccggcatgat
    16321 cgcgactttt tcttgctcga taagcgcttt ttgcaacgcg ttgtcgtcaa tattcaacgg
    16381 acgtagatca agccaggcca gataagtgga ttgcgggatc tgccagttga gttcaggaaa
    16441 cgcggcgttc attttatctg agatatacgt caggttatct ttcagataga cgcgtaaggc
    16501 atccagccac ggtgcacctt gctgataggc ggcgatatgg gcagttaacg ccagtaccga
    16561 aggggaagaa agcccatcac gtcctttcag tgccgataaa taggcatcac ggctgctgct
    16621 attttctata atcccgtaag caccggtcag cgcgggaata ttgaaacttt tcgatcccga
    16681 tgtcagcaac gcccagtctc cccgcgccac attactccag ggaatatgcg gctgctcgcc
    16741 ccagaccata tccatatgga tttcatcgga aataacccgc acatcatgac gctcgcacag
    16801 gtcagccatg atctccagct catcgcacgt ccacactttc ccggtaggat tctgtgggtt
    16861 acacaggagc ataattttac attctggttt cgccaaaacg gcttccagct tgcccatatc
    16921 gcaaaaccag ccgtcagcct gcttctctaa agcaacgggc attactgtgc gctggttacc
    16981 ttcaatggcc ttgtaaaatg cgtcataggc gggcgtgtgg atcaccacgc cttcacctgt
    17041 ttcagaccac tgacgaatca gttctgaaac catatagatg acagaagggc catacaccac
    17101 cgtctgcgta tcaattgcgg tgtaatgctg ggtggaaaac cagtgggcaa tagccgcgag
    17161 aaactcatcg tttttccagc ggctgtagcc aaatacgccg tgcatcaggc gctgattcag
    17221 cgcctcgata atgcaggggg cagtggcaaa atccatgtct gaaatcgtga acggtaacag
    17281 gtcagcagtg ccgaaacggt cagcgacata atcccactgt gtacaccatg tgccatgacg
    17341 atccacgacc tttgaaaaat cgaacatatc ttatccttat gcctggacag tatgcatcag
    17401 accggccatt tcatctttta ctgactgaac ttgtggcccg ataacaacct gcaggttatg
    17461 ttgattaagt tgtactacgc caattgcccg attgtccttc agtgcctgca cattaacaag
    17521 cgacatatct tccacagaca aacgcagacg ggtaatgcag ttatcgaggc tgacaatatt
    17581 gtcggcaccg cctaatgctt cgaggattgc aggaacgttg taacctgatt tacccggcgc
    17641 accggcaacg gctttttcga ttgagctggc aacttcgcta tcgcgccccg gggttttcag
    17701 attgaagcgg gtgatagcga aacggaagat gacgtagtaa acgacaaacc agattgccgc
    17761 caccactggc accatgtacc acttggttga cagaccatgc aaaataccga acaccacgaa
    17821 gtcgatgata ttgccgtcgg tattaccgat ggtgacgccg agcacagaca tgacggtgaa
    17881 gccgaggccg gttaacagcg cgtggatgac atacagaact ggcgctacga acaggaacag
    17941 gaattccagc ggttcggtag tgccgccaac gacgcaggcg atcaggccag aaatcagcag
    18001 acctttaatt ttatggcgat tttccgggcg cgcgcagtga tacatagcta acgctgcacc
    18061 tggtagaccg ccgagaaacg caggcatttt accttgcgaa aggaaacgcg tgacgctttc
    18121 agaaaaaccg tgagtggtcg ggcaactcaa ttgcgcctgg aagatggtca gtgcgccgct
    18181 gacggtttga ccgcagacct cctgcgtgcc gcctgcgtcg gtaaagcgaa ttaatgccac
    18241 cagaatgtga tgcagaccaa acggcaacag cagacgttca ccggtaccaa acagcatcgg
    18301 tccgaaatca cccgcgctgt ttatcatatg gcccaagccg ctaataccca tggcgaaaat
    18361 cggccagact aatggaatca ccaggccgac aaggcccatc accagcgagg agataattgg
    18421 tacgaagcgc gtaccgccga agaatgccag cgcatccggc aggcggatat tatggaaacg
    18481 ctcatgcagc atccagacga taataccggc gatcaccgca ccgaggatcc cggtatcgat
    18541 cgactggatc ccaagaatgc tctggatgtt attggctttc agaacggcgg catctgtggt
    18601 tggcagaatg cctttattgg tcaaccagaa gtttaccgcg aggttcatta ccgcataacc
    18661 gacgaagcca gcgaatgccg ctacgccttt attttcgcgt gccaggccca gcgggatggc
    18721 gatacagaac atgacaggca ggaaactaaa agcaaacgag ccaatcttac tcatccaggt
    18781 aaagatagcc tgcaacacgg ggttgcccag gaccgggatc agggttatga catcatggct
    18841 gctaagagaa ctaccaatgc cgagcataat gccgcagaac gacaataatg ccacgggtaa
    18901 catgaaggtt ttgcctaact gctggaagaa ctcccacagc gtgactttcg gtgctgtttt
    18961 cgccgtcata aaacgactcc tcgtagagaa caatctggat tcagtaaatt gcgcgagaag
    19021 ataaaacgtt ttatcaaatt ttagtgagtc ataaatcaca ttacgcaacg ataatagcgg
    19081 gtataagata aataaaaggt aaaacgtttt atctgtcaca taatcaggga gtaggtcatc
    19141 tgcatggcta ccgccaaaaa aataaccatt catgatgttg cgctggctgc gggcgtgtcg
    19201 gtaagtaccg tttcgctggt gcttaatggc aaagggcgaa tctctaccgc cacaggagaa
    19261 cgcgttaacg ccgccattga agagctggga tttgtgcgca atcgacaggc gtcggcgctg
    19321 cgcggcgggc aaagcggcgt cattggtttg atcgtccgtg atttatctgc gccgttttac
    19381 gccgaattga cggccggatt gacggaagct ctggaagcac agggacggat ggtttttttg
    19441 cttcacggcg gtaaagacgg cgagcagctg gcacagcggt tttcactgtt actgaatcag
    19501 ggtgtcgatg gtgtggtaat tgccggggct gcaggaagca gcgatgacct gcggcggatg
    19561 gcagaagaaa aagctatccc ggtgattttc gcttcccgtg ccagttatct tgatgatgtt
    19621 gatacggttc gcccggacaa catgcaggct gcacagttgt tgacggagca tctcattcgc
    19681 aatgggcatc agcggatcgc ctggctggga gggcaaagtt cctcattaac ccgtgcagaa
    19741 cgggtggggg gctattgtgc aactctacta aaatttggcc tgccgtttca cagcgattgg
    19801 gtgttggagt gcacttccag ccagaagcaa gccgcggaag ctatcacggc gcttttacgt
    19861 cataacccga ccatcagtgc cgtggtttgc tataacgaaa ctattgcgat gggggcatgg
    19921 tttggtttgc tgaaagcagg gcggcaaagc ggggaaagcg gagtcgatcg ttactttgag
    19981 caacaggttt cgctggcggc atttaccgat gcgacaccaa ccacacttga tgatatacct
    20041 gttacctggg ccagcacgcc agcgcgggaa cttggtatca cacttgcgga tcgcatgatg
    20101 caaaaaatca cccatgaaga gacgcattca cgcaatctta ttattcccgc ccggctcatt
    20161 gcggcgaaat aatcctctct ttatctgcta tacttggtag tgtcctttcc tcaaggttaa
    20221 tgataaaaaa tgtggcacac aggcggtgcc ttttattttt actgaagttc acaggaggtt
    20281 tatgtgttta attctgacaa cctgagactc gacggaaaat gcgccatcat cacaggtgcg
    20341 ggtgcaggta ttggtaaaga aatcgccatt acattcgcga cagctggcgc atctgtggtg
    20401 gtcagtgata ttaacgccga cgcagctaac catgttgtag acgaaattca acaactggat
    20461 ggtcaggcat ttgcctggcg ttgtgatatt acttccgaac aggaactctc tgcactggca
    20521 gactttgccg tcagtaatct gggtaaagtt gatatcctgg ttaacaacgc cggtggcggt
    20581 ggtcctaaac cgtctgatat gccaatggca gattttcgcc gtgcttatga actgaatgtg
    20641 ttttcttttt tccatctgtc acaacttgtt gcgccagaaa tggaaaaaaa tggaggtggc
    20701 gttattctga ccatcacttc tatggcggca gaaaataaaa atataaacat gacttcctat
    20761 gcatcatcta aagctgcggc cagtcatctg gtcagaaata tggcatttga cctgggtgaa
    20821 aaaaatattc gggtgaatag cattgcgccg ggggcaatat taaccgatgc cctgaaatcc
    20881 gttattacac ca
//
LOCUS       DAECOD010000069        20807 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.069, whole genome shotgun sequence.
ACCESSION   DAECOD010000069 DAECOD010000000
VERSION     DAECOD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.069"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            89..670
                     /locus_tag="KKO15_002581"
                     /pseudo
     CDS             89..670
                     /locus_tag="KKO15_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            688..1326
                     /locus_tag="KKO15_002582"
     CDS             688..1326
                     /locus_tag="KKO15_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6361327.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1617..2345
                     /gene="mpaA"
                     /locus_tag="KKO15_002583"
     CDS             1617..2345
                     /gene="mpaA"
                     /locus_tag="KKO15_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBD6361328.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2320..3285)
                     /gene="ycjG"
                     /locus_tag="KKO15_002584"
     CDS             complement(2320..3285)
                     /gene="ycjG"
                     /locus_tag="KKO15_002584"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBD6361329.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3404..3910
                     /gene="tpx"
                     /locus_tag="KKO15_002585"
     CDS             3404..3910
                     /gene="tpx"
                     /locus_tag="KKO15_002585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBD6361330.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3954..5495)
                     /gene="tyrR"
                     /locus_tag="KKO15_002586"
     CDS             complement(3954..5495)
                     /gene="tyrR"
                     /locus_tag="KKO15_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBD6361331.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5643..6704)
                     /locus_tag="KKO15_002587"
     CDS             complement(5643..6704)
                     /locus_tag="KKO15_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBD6361332.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6701..8098)
                     /locus_tag="KKO15_002588"
     CDS             complement(6701..8098)
                     /locus_tag="KKO15_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBD6361333.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8254..9252
                     /locus_tag="KKO15_002589"
     CDS             8254..9252
                     /locus_tag="KKO15_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6361334.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9363..10235)
                     /gene="ompG"
                     /locus_tag="KKO15_002590"
                     /pseudo
     CDS             complement(9363..10235)
                     /gene="ompG"
                     /locus_tag="KKO15_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10308..11389)
                     /locus_tag="KKO15_002591"
                     /pseudo
     CDS             complement(10308..11389)
                     /locus_tag="KKO15_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11403..12062)
                     /gene="pgmB"
                     /locus_tag="KKO15_002592"
     CDS             complement(11403..12062)
                     /gene="pgmB"
                     /locus_tag="KKO15_002592"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBD6361335.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(12059..14326)
                     /gene="ycjT"
                     /locus_tag="KKO15_002593"
     CDS             complement(12059..14326)
                     /gene="ycjT"
                     /locus_tag="KKO15_002593"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBD6361336.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14323..14946)
                     /locus_tag="KKO15_002594"
     CDS             complement(14323..14946)
                     /locus_tag="KKO15_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6361337.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14943..15365)
                     /locus_tag="KKO15_002595"
     CDS             complement(14943..15365)
                     /locus_tag="KKO15_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6361338.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15387..16098)
                     /locus_tag="KKO15_002596"
                     /pseudo
     CDS             complement(15387..16098)
                     /locus_tag="KKO15_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16116..17168)
                     /locus_tag="KKO15_002597"
     CDS             complement(16116..17168)
                     /locus_tag="KKO15_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBD6361339.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17199..18041)
                     /locus_tag="KKO15_002598"
     CDS             complement(17199..18041)
                     /locus_tag="KKO15_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBD6361340.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18028..18909)
                     /locus_tag="KKO15_002599"
     CDS             complement(18028..18909)
                     /locus_tag="KKO15_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBD6361341.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18930..20213)
                     /locus_tag="KKO15_002600"
                     /pseudo
     CDS             complement(18930..20213)
                     /locus_tag="KKO15_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20227..>20807)
                     /gene="ycjM"
                     /locus_tag="KKO15_002601"
     CDS             complement(20227..>20807)
                     /gene="ycjM"
                     /locus_tag="KKO15_002601"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBD6361342.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5081 a         5667 c         5064 g         4995 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg tggctatgcc attaatgccg cgctgacaga
      121 aaaacgaatt aaagctgtag tgagtattac gggtgttaat atcgggcgtc tttttcggga
      181 aggtttcagc aattatgatc cgattggcgt attgaatgca atggcgtcac aacgagcaaa
      241 agaggctcgt ggtggagaat tacagatcaa cgaattactt cccgccagcc tggatgcagc
      301 aaaagcccat ggactgaccg aacgcgatgt ttatgaagca actgactact ataaaacacc
      361 acgcgggcaa caaccgggag gtgcgacaaa aatgttgttc tcacatgcac agaaaacgct
      421 ggcctgggat gccttcgcat ttactgaagt gttactgacc cagccggtga tggtagtagt
      481 tggtgaaaaa gtcggtgcct tcggtgctta tcgtgatgga ctggaagtct atggccgggc
      541 gatggtttcg caggatcgtc agttagtttc gttacccgat ttctcacatt atgaactata
      601 tgacaaaccg gaagcagttc aggaggcgct ggcaaaagtt attccttttt tcaacaccca
      661 tctgggataa gtttcatgag aaatattatg aaaaatgtac tgattcttgg tgctggcggt
      721 cagatagccc gccatgtgat taatcaactg gcagataaac aaactattaa acaaacttta
      781 tttgcccgtc agccagcaaa aatacacaaa ccctatccaa ctaatagcca gattattatg
      841 ggggatgtac tgaatcatgc agcactgaag caggccatgc agggacagga tattgtgtat
      901 gccaacctta cgggggaaga tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa
      961 gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg gtatttatga tgaagttcca
     1021 ggaaaatttg tcgaatggaa taatgcagtt attggtgaac ccctaaaacc attccgcagg
     1081 gctgcggatg caattgaggc ttctggactg gaatatacaa ttcttcgccc tgcctggctg
     1141 actgatgaag atattattga ttatgagctt acctctcgca atgaaccatt caaaggtacg
     1201 atcgtatcac gtaagagtgt cgcagcatta attacagata tcatcgataa acctgagaaa
     1261 cacattggtg agaatatagg catcaatcaa cccgggactg atggcgataa gccattcttc
     1321 atgtaaatca attatataga taccatcgta ctgtactatg tgcagtacga tttattaaac
     1381 ttattaaggt gaactgatca atcagatgcg attaacatgt gtagaaccag taagaataaa
     1441 atacgccatg tatataacat tacgacaatt ctaatatctg cacatcaatg aaaatgcaaa
     1501 caacttccgg caaaaaagtg atacaagatc gatgacatag cgttgaagaa aagtatttga
     1561 taatccgcaa aatttgcaca acactgcctg taatacttcc tgaaccagag acgatcatga
     1621 ccgtaacccg ctcacgcgcc gaacgcggcg catttccgcc cggaacagaa cattacggac
     1681 gttcattatt gggcgcgccg ttaatctggt ttcctgcccc tgcagccagc cgcgaaagtg
     1741 gtttgattct ggctggcact cacggtgatg aaaactcttc ggtcgtcacg ctctcctgtg
     1801 ctctgcggac tttgacacct tctttgcgcc gccatcatgt ggtgctgtgt gtgaatcctg
     1861 acggctgcca gctggggtta cgggccaatg ctaatggtgt ggatttaaac agaaactttc
     1921 cggcggcgaa ctggaaggaa ggtgaaacgg tttatcgctg gaacagcgcc gctgaagaac
     1981 gtgatgtcgt attactgaca ggtgacaaac ctggctccga acctgaaacc caggcgctgt
     2041 gccagcttat acaccgcatt caacctgcct gggtggtctc attccacgat ccactggctt
     2101 gtattgaaga tcccagacac agcgaattag gcgaatggct ggcccagtcg tttgaattgc
     2161 ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt cggcagttgg tgtgcagatc
     2221 tcaacttgca ttgcatcacc gcagagtttc cgcccatctc ctccgacgaa gccagcgaaa
     2281 aatatctctt tgcgatggca aacttgctgc gctggcatcc taaagatgca attcgcccgt
     2341 cgtgaactga agcgccggtt ccacatctac cgccagccag gtcggtccgt caagatcggc
     2401 gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg gcacgagagg tacacaacat
     2461 gcagcccagc atcagactga aaccttgtgc acgcgcttca gtcgccagcg ccagcgcttc
     2521 cgtcagacca ccggttttat cgagcttaat gttaaccatc tcatagcgcc ctttcagcgc
     2581 cttcagattg ctacgagtat gacaactttc atcagcacaa atcggcaacg gatgaataaa
     2641 attctccagc gccgcatcgt cctgcgcagg aagcggttgt tcaagcatcg caacgcctaa
     2701 atccgccaat agctggcaac gcgccgccaa cccttctgca cgccaggatt catttgcatc
     2761 aacgatcagc gtggcatcgg gcacagcggt gcgaattgcc accatccgct cactgataag
     2821 atggttatcc agcttcactt tcagtaattt cgcgcctgcc tgccagagtg ttgatgcact
     2881 attggccatc tgatcaggcg taccgatgac aaccgtctgt gcagtgataa ctgtctcggg
     2941 aagcgttatg ccgatcaaat cagccagcga ttgctgctgt tttcgcgccg cgagatccca
     3001 caatgcacaa tccagcgcat tacgtgctgc gccggcaggg agaatttttt gcaactcctc
     3061 ccgtgtcagc cctttctcta attgcggcac gacgctcata atttgcgcca ttaccgaggc
     3121 atcactttct ccataacgcg gatacggcgt gcattcgccg gtgcctttaa taccctcttc
     3181 ttccagttca accaccacca cgcgcgcttc actgcgactt ccccgggcaa tcacaaacgg
     3241 ggtatgtaag ggccaggctt cctcaaatac cttaacgatt ctcatcttta ctccttgttg
     3301 tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg tgttattgac agttagcata
     3361 aactaggtgt gacgttaact atatgtaaac aggaaagata atcatgtcac aaaccgttca
     3421 tttccagggc aacccggtta cagtcgccaa ttccatcccg caggcgggta gcaaagcgca
     3481 gacttttact ctcgtggcaa aagatctgtc tgacgtcacc ctcggtcagt ttgcgggtaa
     3541 acgcaaagtg ctgaacattt tcccgagtat tgataccggt gtttgcgccg catcagtacg
     3601 taagtttaac caactggcaa ctgagatcga caacaccgtt gtgctgtgta tctctgccga
     3661 tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct
     3721 ctccactttc cgtaacgctg aatttctgca agcctacggt gtggcaattg ctgatggccc
     3781 actgaaaggt ctggcagcgc gtgccgttgt ggttattgac gaaaatgaca atgtgatttt
     3841 cagccagctg gtggatgaaa tcaccaccga gccggattac gaagcagctc tggctgtact
     3901 gaaagcataa gttaatatgc ctgatggtgt tgcaccatca ggcatattcg cgcttactct
     3961 tcgttcttct tctgactcag accatattcc cgcaacttat tggcaatcgc ggtatgtgaa
     4021 acgccgagac gttttgccag tttgcgcgtg ctgggataat tgcgataaag ctgggttaat
     4081 accgagcgtt caaaacggct ggtgatttcg tccagcgaac cttccatcgc atcttcgccc
     4141 acggctaccg ttgcggcgtc ataatccggc aacaaaatat cctgtggacg cagctcataa
     4201 ccgtccagtt gtgtcagtgc gcgatagata gcgttcttta actgccgcac atttcccggc
     4261 cacgcataac gtgtaagtac agtattcagg tcagcggcca gtttcggacg cggcacgccc
     4321 tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg gcataatgtc ctgcggacag
     4381 tcacgtagcg gcggcagatt gagcgtcaac acgttcagac gataatagag atcttcacgg
     4441 aacacgcctt tttgcaccag ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca
     4501 tcgacatgca cctcatggtc ttcgccaacc cgacggaaag tgccatcatt aagaaaacgc
     4561 agtaatttcg cctgcatccg tggtgacatt tcccctattt catccaacag caccgaacca
     4621 ccgttcgcct gctcaaagaa ccctttcttc ccttccggag catgaccaaa cagttcactc
     4681 tcgaccgcat cttctggtat agacgcgcaa ttcagcgcca ggtaaggttt gcccgctctg
     4741 gggcttgcct gatggcaggc gtaggcaaaa agatctttac ctgtacctgt gtcacccgta
     4801 atcagcagcg gcgcgcttag catcgccagt ttctgcgcct gttcgacaac gtgcttcatt
     4861 ttcgggctga cggcgacaat ttggctgaag gcgctgacgt cctgggcggc gacattttgc
     4921 aactggcggc ccatacgaat cgttgatcgc aacatcacca ccgcaccggt caggacgtgt
     4981 tgatcatttt catcctgaag ataaacaggc gtaatctcca tcaggaaatt ctgcccatta
     5041 ataacgacat gctcgttatg cgaatcttgc ggttcgcttt ccagccaacg taaaaaatta
     5101 aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc gatccaattt ttgcccaaaa
     5161 agctgacagc tcgccgggtt cgccatatcc actttgcttt tcatatcgac agagagcaca
     5221 ggttcaggca acgcctccag taacgcgctc aacgccagat gctcacgttc ggaaggcatc
     5281 cacgggacag tacgcacatc ggtaacacct gcaatacggc gtatttcggc catcagactg
     5341 ctgaaactct caaactccag ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc
     5401 tcaataccgc gtaaatcaat gcctcttagc acgagtagat cgagtaattc gcgggtcaga
     5461 ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa ccttcacctg aaaaaagaac
     5521 aattaatatg atgatatgac actatttgga tagcaggaag gtttctgtca acaatcattg
     5581 acacaaagac ggaaaaacgg gcacttcggc gtaaagatat ccggtcagca cccgatattg
     5641 cattattttt cgctgggcgt tttgcctttt tgcagagttt ctttcacctg accgataagc
     5701 tgacgacgga aatccccaag gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa
     5761 agctccatag ctttaatccc gagtcgtacc gtcagaagtc cagcaccaat tccctgagct
     5821 gcgcgggtag acaaacgagc agcgagatct tgcgacatcc agtccatccc cacttcgcgc
     5881 accagttcgc tggctccggc aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa
     5941 cggctgtaat accccagttc aatgccatac agcgtggcaa tgcgattaat taaacgcaga
     6001 ttgcgccagg cgataaacgc catatcgacc aacgccagcg ggctgaccgc aatcatcaac
     6061 gttgattccg ccgccgaatg gctgatttcg cgccgcgcct gggcatctaa aactggctgg
     6121 accaaatgcg catacaggct gaccacttca cgatcgttct gcgtttcatg gattgaggca
     6181 taccagcgtt gcagcgccgg atgcgactga tcaatacccg cctgctgcgc cagtttttcg
     6241 caaaatgcgc gacctttgcc cgtgccgtgg ctatgcaata aatcacgcgc ttcgtcgcgt
     6301 tcatgggcgc gctgtcgcaa gcgccataag cgccgccact ctgttaccac agaacctacg
     6361 ccagcgccga taatcaatgc cccagcggca catccaccca gcgctaccca gtcctgggtt
     6421 tgccaggcat tcattgtcca ctgtataccc tgcccgacaa cgcttgcgcc aaacagagcc
     6481 agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc tgcgtccatt
     6541 accgcttcga cttgcccctc ttcttcctgc gcttcgtcga gcgtggccgg ggcaaaattt
     6601 tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg ttcgacctcc
     6661 agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat ttatctccta
     6721 ttaaaaattc cagcgcggca tcaagacgaa tatgcggcag cggtttgtcg acatccatca
     6781 cctgcgggcg aaacgcttca aactggaacc cttgcttgtc ccagaacgcc tgaccgggca
     6841 aacgcgcagg aacttcaccg gggtaaacgg tgagaggcgc accatcgctg agccggttac
     6901 cgcgcaatgc cggaattttc tcgccgttga cgtcaataat gccgctggtg gtcgcctgca
     6961 ccgaggccag ccccaggcaa tccatactaa tcctttcaaa cgccgcattt tgccaggcat
     7021 cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgacc gttacatggt
     7081 ccgctttcat cgcggcaaac agcagtttgt cgataaccgg cgagaataag cggcgaaaca
     7141 gcgtgcgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga cgcatatcat
     7201 taaatgcctg tggcccactg ttgagaggtt gcaggcaatc caccagcaca atctggcggt
     7261 caaagcgcag aaaatgattc ttatagaacc ccttcaccac tttctcgcag taataattaa
     7321 accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt ttggactcgt
     7381 cccaggcatc gacatccggc cacgggaaga attgcagcgc gggcgcacct gccatatctc
     7441 ccggcaagac aaagcgccca ggctgaataa agtgcagccc ctgctgttta cagtggtgga
     7501 gataatcggt ccacacggcg gcaatgtctg ccagccggtt ttcgtcggca ggcgctagcg
     7561 ggtcaagtcc ttcgcacatc attcgccatt tcgccgacca ttcgccgcgc tgaccattga
     7621 gtaagcccgt catctggcgc gaccagctta agtaatcctg cgccagcatc ggcaggtcga
     7681 gcaaccattc gccgggataa tccacaattt ccagatacag cgtggaggta tccttaaagt
     7741 ggcgcagcag cgaatcgttc gatttaaaac gtaacgccag gcgaatttca ctgacgccgc
     7801 gcgtaggcgt cggccaggcg ggaggatcac catataactg cgccagtcct tcatcatagg
     7861 tgaagcgcgg aatgccaaag tcacgctggg gaatgcgttt tacgcccagc aagcgctctt
     7921 cacgcaccgc gcttaacagc ggcaaacgtg ctccggcatg aatattgagc aactgattaa
     7981 ccatcgcggt gataaacgct gttttgccgc tgcggctaag tccggttaca gcgaggcgca
     8041 gatgtctgtc gacaccccga ttcaccagcg cattgagttc atttttaagt cgcttcattg
     8101 ctgtcctgtg ctgctctgaa gaccagaaaa aaaatcattc gtaaggatat gaatgtgggg
     8161 gccaggccat tgattatcaa ctgcccgtgt tgtcatcgac gggcgaactc cataagatgt
     8221 gcagcattca cgttcggacg gagttcctga cctatgtccc ctactattta tgatattgcc
     8281 agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc tgaataagca aactaatatc
     8341 tccccggaag cgcgcgaaaa agtgttacgg gccattgaag aattacagta tcagccaaac
     8401 aagctggccc gcgcgctgac ctcttcgggt tttgatgcca ttatggtgat ttctacccgt
     8461 tcgaccaaaa ctacggcggg taatccgttt ttctctgaag ttttgcatgc catcaccgcc
     8521 aaagcggaag aagaaggttt cgacgtgata ttacaaacgt cgcacaatcc ggcagaagat
     8581 ctgcaaaaat gcgaaagcaa aattaaacag aaaatgatta aaggcattat tatgctcagt
     8641 tcgccagcgg atgagtcatt ttttgcccaa ctcgataaat acgatattcc ggtggtggta
     8701 attggcaaag ttgaaggtca atatgcccat gtttattctg tcgataccga taattttggt
     8761 gacagcattg cgttgaccga tgcattaatt gaaagtgggc atcaaaatat tgcctgcctg
     8821 catgctccgc ttgatgtcca tgtttcggtt gatcgcgtta atggctataa gcaaagtctg
     8881 tccgcgcata atattgcagt gcgtgataaa tggattgttg acggcggtta tacccatgaa
     8941 acagcactgc aagcagcacg gcaattatta agtcagtcac cgttgcctga ggcagtgttt
     9001 gccactgaca gcctgaaatt aatgagcatt tatcgtgcgg cggcagagaa aaatattgct
     9061 attccgcagc agttagcggt ggtgggttat agtaatgaaa cgctgtcgtt tattttaacg
     9121 cctgcaccag gcggcatcga tgttccgacg caggagttag gtcaacaaag ctgcgagtta
     9181 ttattccgct taatttcggg aaaaccgtca ccacagaata ttaccgttgc cacgcatatg
     9241 acgctgaaat aaataccagc taccttgcct gaatgccgga tgcgatgctg gcgcatctta
     9301 tccggcctac atttatagat cggataaagt actcgcgccg catccggcaa tttagcccat
     9361 tatcagaacg agtaatttac gccgacacct gcataatgga atttatcgct gtcgccttcg
     9421 tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg aaagtccgtt ctggaaatca
     9481 taaccgtaaa ataaacccac acggttaaaa tcatggcctt cacgttcaat atcatcctgc
     9541 cagtcccagt tactctagcg atccagccca atgcgcgtat acggcgtcac cgagtggttg
     9601 ccgagcgtca gcggcaaata ggcgcgaatt tcttgtgtgg aaaactcacc gttattgcgg
     9661 ctgtcgtcca tattgaagcc gcgctcgaga taatagttca ctcgcaaagc aaccgtttcg
     9721 ttgaaggtat attgcagacc tgtttccgtt tcgacacggg tatcagcgta accggtcgtg
     9781 ttcaaatcgt tggcaaattt atacatcgac aaccaaccgt tgaaacgtaa atcatcagtc
     9841 agtttcacat cccagtctgg cgcgattttc cagcgctgca tattcgccgt gtctttgccc
     9901 ggttcatcaa cgtagtgata accataatta cggaaaccgc cggtcaggcc gaaactgaaa
     9961 tcatcgcttt cgaggaactg ataatgcacc tccagctccg ggcgatcaaa cctcgttcca
    10021 cgtttacccg cgctataatc taccggccct tcctgataat aggccagagc aattctccac
    10081 ggcccgttgg cggcattaaa atagactgaa ggctccgcca gcccatccat atcttcgcca
    10141 taaccctcga cgttttctat ttcgtacatc gcaccgatat taaagtgcca gtcgctcttt
    10201 tcctcggcct gtgcgcaggc cattcccgca cacatcacca gtgcggtaca gggtaataac
    10261 tttttttgtt ccttgtatta tcctggggga atgccttccc cctgtattta gcgaattgct
    10321 atttccgttt ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat agtgatattt
    10381 tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg cccccaaccg
    10441 tggtgtagag cataaattcc gcaccggtta attctgccac gctaattttg gcggaaatgt
    10501 tattttcctc ttgcgcgtcc ggatgaatat cttccgggcg tattcccatc acgatgggct
    10561 tatgcaaact ttcctgtgtt ttcagaaccg ctaatttctc ttcgagaatg gttaatttaa
    10621 gcgtttccgt aacgaattta tcgccatcga tcgtgccgcg aataaaattc atcgctggtg
    10681 atccaataaa tccggcaaca aacatattcg cgggttggtt ataaacggtt ttcggcgcac
    10741 ctacttgctg aacaatcccg tctttcataa tcacaatccg cgccgccatg gtcatcgctt
    10801 cggtctgatc gtgggtcacg tagatcatgg tggtgttcag tttctgatgc agcttgctga
    10861 tctctgcacg catttgtacg cgcagcttgg catcaaggtt agagagcggt tcatccatta
    10921 aaaacacgcc cgcttcgcgc acaatcgccc gcccgagcgc cacacgctgg cgttgcccgc
    10981 cggaaagcgc gcccggcttg cgtttcaggt actcacgcag gccgagaatt tgcgccgccc
    11041 agttcacccg ctcatcaatc acctctttgg cgattttttg catcttcagg ccaaacgcca
    11101 tgttgtcgta aaccgtcata tgcggataca acgcgtagtt ctggaacacc atcgcgatat
    11161 tgcgtgcttt ggctggaacg tcattcattc gtttgccgtc gatcaacaga tcgccgccgc
    11221 tgatctcctc aagcccggca atcatgcgca gggtggtcga tttaccgcag cccgacgggc
    11281 cgacaaatac gatgaactct ttatcggcaa tttccaggtt gaagtccttc accacatgca
    11341 cctggttatc gtagattttt tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt
    11401 tgctatacgt tttgccagaa ggccgataac cgcgaccagg tcagggagtc cgttgagggc
    11461 aacagtaatt gcgccccggt taagcccgca ccgatcccca ccgagcgcat accgctggcg
    11521 ttaatggcgt caatgcccgc ctgcgcatct tcaatgccga tacatgcctg cggcggcacg
    11581 cccagcccgg cacaggcagc gagaaagatt tccggatccg gtttcgagtt tttaagttgg
    11641 gaagcatccg cgcagaaggt gaaaaactcg cgcagctcca gcgccgctaa aatcgtcggc
    11701 gcattcaggg agacggaagc cagccctacc ggaatctgct gtgcacggag ctctgccagc
    11761 aaattgcgaa taccgggcag cacagcatta accgtcagct cgcgcagtga gtggacgtaa
    11821 agcagatttt tgcgatacgc cagttgcgcc cgctcctgcg ggttaaagtc gccctctttg
    11881 cccccgtgtt gcagaatgcg ccgcagagac tcatcgcggc tgatcccttt tagagattcg
    11941 ttaaactgcg catcaatact gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa
    12001 tgcagatgcg cggtatcggt gattacacca tccagatcga aaattacccc ttgcagtttc
    12061 attcatcctc ctgatgcgtg gtagcggtcc cattgaaggg caaaataaaa tcacccaaac
    12121 agaaaacaga ttcttcagcc acggatataa gctgaccgtt caaacgcagt gaaacgggcg
    12181 cagaagttcg aatcgcaata cgctgcgcgt cgagagtgac ctgtaattcg cagccctgcc
    12241 agaacagagg gaaagacaac tgttgccact gctcaagtaa cgccggattt agatgcaatt
    12301 caccgtcacg cacgctcacc ccggcaaaac cctgaatcgc ccccagccag atagcgccag
    12361 ttgcggcagc atggatacca tcatcacaac tatgcggatc agcaccaaga tcgatttcag
    12421 tcccctcgcg ccagaactga taactttggg tcagcaggcc acagcgtgca gcaacaatgc
    12481 cgtggattgc tttacttaat gacgagtcgt gaatagtgcg cggttcataa aattgcagat
    12541 tggcaagaca cgatgccgat gagaactgct ctggcagcat gtaattgagc atcaccacat
    12601 cagcttgttt gaggatctgc atctcgttca cttctgcgcg tgaataatcc agcagaatgg
    12661 tttgcttccc cgccgccgct ttgtatttcg ccagattaat cgccggctta gtcataaacg
    12721 aatcatcctg cggcaaaaca ccgtcgggct gaatttctgg cagccgcagt tctttaagga
    12781 acatttcggc acgatgaata aacgcgtcgt cactacagcc aaactggcgg gcaatactca
    12841 gcgcctgctg gacgttgtaa tacaccatat agctggtgaa ggcgttatta ttgacatgtt
    12901 cggtatattc gtctggccca ataacatcat gaatttccag acggtcgtta accctcaccg
    12961 cgcggctaat ccagaacttt gcagtttcca gaagtagcgc catgccttca tgcgcaatga
    13021 aactttcatc ccccgtggtc tgccagtatt gaataaccgc ccaggcgata tcggccacca
    13081 gatgatgttc cgcctgcgcc gaggccactt tttgccgcag cccggtgcga atgttaatgg
    13141 cggcaaattc cggcgtctct tcttcgccgc tgcgcgcgct ttcccacgga aatagcgcgc
    13201 cctgccagcc gttgcgtcgc gctttctcct gcgcgcctgg caagttgtgc cagcgataac
    13261 gcagtaaact tcgggcaacc gtcggatcgc taaacagatg gaacggcagc aaaaatactt
    13321 ctgtatccca gaaaacgtgg cctttgtagc cttcgccggt taagcctttt gccgcaatac
    13381 tgctgcgctc gccgtgagcc ggcgtcatga tgcgcagatg ataaagcgca taatctaacg
    13441 cttgctgatc gtgcgcatcg ccgccattta ccgtgatacg acgtttctgc caccattgac
    13501 gccagttttc tgttgatgct gcaagatgtt ggtcgtaact ctgttgcgcg cacatttcaa
    13561 gctggcgaag cgacacgctg ccccactcgt ctaaagcagc ttgcctgtca tcccgccagt
    13621 cgatccacac cagtttttgc aacgtcactg tctcgcctgc atgaagctgc gtactggtat
    13681 gttgcagtaa acggcgctct ttggcggtat agcattgctg cacatcaccg ctcacctggc
    13741 agcaacaact gatggccaca tcactgcgcc catcctgggt ggtgtagatc ccctgcatca
    13801 aatgctgacc aaacacccgc acctgggttt cgtcgagatg ctggcgaccg tggttggttt
    13861 gcgtggcgtc aatgcctgtt gaaatcagca ctgacgcgtc ggcgtccagt ggcataatag
    13921 tgatttccag cgcaatgagc ggcagttggt ctgccgaaac aaagcgacgg ctggcgatgg
    13981 tgtaacctgc gccgttgctg gtacgccaga caacgctacg gcgtaattct ccactggcaa
    14041 agtcaagttc acgctgccag gcttcgcggg ataacgagaa aacctcacca tttatggcaa
    14101 tctccatccc caagatatca ggcagattca ccagttcgtt gatttcacct tttcccgccc
    14161 gatgatacag ccccgccaga tacatccctc gcgtctggcg cgtgtaatct tcttcatggc
    14221 tggcgcgaag cccaagatag ccgttcccct gcgccatcag cgatgcatac ttgttcaggg
    14281 tatgctggct gaaatggggt tctgataacg ttactggcct ggtcataatt ccacacgcgt
    14341 ccctgtttct gcggattgat ataacgccgc caccagttgc tggatgatat acccctgctc
    14401 ggcgtcggct atcatcacgg gcttgccctg tacgtgattg ataaaggctt ccatgctgcg
    14461 caaatggcgg ttgtcgtctg ccatttcccg ttgcatcagc gtcattaatt caccgttgtt
    14521 atcggtgtag atatgtgctg gaaacagcgt cgcaccggct ttatcaccac aaaagctgac
    14581 gttcataatc aactgttcgc ggatgttgag tgcaaatgac gtttccagcc acagaatgcc
    14641 gccgttatga aattcaatgg tgccaaacag cgaatcttcg acgctgtaag ttgccggatc
    14701 ccactcgcca aattgaccac agctcttttg cgtgccgatc ttttgaaaac tatgcgcatt
    14761 cacgcttttc accgccggaa agcccagcac atacatcgca gcatccagca tatgaatgcc
    14821 gatgtcgatc agcaggccac caccctgcag ttctttattg gtaaagacac cccagccggg
    14881 aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa atctctccca aaacgccgtt
    14941 ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac gatggtgaaa gtcgtaggcc
    15001 agcaccttgc ccagtttgcg cgcggtatcg cacatttccc acgcctgttc tgtcgtcatg
    15061 gcgggcggtt tttcgcacat cacatggcag cctgcttcca gtgccatcag ggtatgttcg
    15121 taatgaaaac ggttaggtga gccgacgcta accacatcag gtttcaccgc cagcagcatg
    15181 gcctgcggat cgtcccacac ggatgcattc ccgtattttt ctgccagcgc ctgcgcctgg
    15241 gaaaggcggc tgtcacagac agccaccagt tccagatcgt tgcgggtgca gtagtacgaa
    15301 gcatgaacct tatccgccac ctggcccgcg cctattatcg cgacccgcag cggagagctt
    15361 gtcattgcac ttttcacaaa gacctcttag caggtacgca accaggccaa cgaatcacgg
    15421 tacgcctggg caggatcttc tgcccggata cgcccttcat acaccacata accctgatag
    15481 ttatcggcgc gcagctgttc aaacagcgcg tggaaatcga gggtgccact gcccggctgg
    15541 taacgatggt tatcggcaat atgcacatga ccgagcaagt cacggttgtc gtgcagcgcc
    15601 tgcgccaggt tatcttcttc gatgttcatg tgatagaaat cgccgataat ctgcacatgt
    15661 ttaagattgt tttcgacgat gtaacgacga gcatcggcga gggtgttgat catatgatcc
    15721 tgatagcggt ttaacggttc gagatacacc acggttccgg tacgcgcggc gaggtcatcg
    15781 gcggtaagcg gaaggtaaac atgccccacg ctgccggaaa ccccccactt ctgccagcgc
    15841 ctcgagaatg cgttcgatct gctttaagcc attaagacga cgctcttcga taaagtcacc
    15901 aatccagccg tcatagccgc cgcaggcggt ggtcactggt aaaccggttt ctttgatcgc
    15961 cgctttgact tcttcgatgt tgttgaccag cagtttgccg tcaatctcaa aaccatcgaa
    16021 gcccatctct ttgatataac gaaatctctc gcggatattt tccggaaaga atgcctgatt
    16081 ctgtgtgccg attttcattt tttcattcct ttgaattaaa acgtaacgcc cattttgatg
    16141 ctctgttccg gatgctgatc gacatactgc atgtagcttt ccgggctgtt ggcaaaggtc
    16201 actaccgggt cgattaaatc ttcgcaattg agataaccgt tcatcagcag ttcccagcag
    16261 gtttcttcaa tacgcttacg gctccagcgc ggataatccg ggttcggttc gctgcacgcg
    16321 cgggagaaaa caattttggc gttattgaaa tgcgcttcgc gtccgaggtt aaaaccttca
    16381 gcaaatggtt tggcaaacgc gacataggag atggtgccgc cgtaggccag gccgcgcagc
    16441 gccgattgca gcgcgtcggc gtaaccgctg gtttcgataa tcacatcagc cccctgcttg
    16501 ccggtcagcg ttttgatctc tttgcctacg tcagtgccaa tggggttaag gcagaaatca
    16561 gcaccgtggc gacgggcaat atcacagcga tgggcgatag gatcgacgcc aattaccacc
    16621 gatgcgccag cgcgtttcgc cagttggatg gcaatttgac cgatcgcgcc aagccctacc
    16681 accacgacaa aatcccctac gcgtacgttg gcatcgcgca cgccgctcat ggcaaactgc
    16741 gccgggtcgt aacagacggc gtttttccag gagctgcctt gcggcatttt gcgcagcttg
    16801 tagttattca ctgcgttaat gatgaccgtc tcggagagcg ggccgtagcc gcatacgctg
    16861 tcgcccaccg cgtagtctgt aacgtcgctg ccgcactcga taatgtcgcc aaccaccatg
    16921 ttgccaagct ggaatttgcc aaactcaatg ccgcgcggcg catctgccgg acgcggggtg
    16981 aacatctgcc actcgccgtt aaagtcttca tcgataaacg ggctggcggc gcggaagtcg
    17041 accacttccg ttccgtgttt cggtgcgccg aaacgcacgc ggatcttcac ttcattggct
    17101 aaaatcgccc gatcttcata ctcaaccagc gcagcaacac gcggtgctgt ggctactaac
    17161 tttttcatgt aaaactcctt gtaaagaaac tggccgcttt agcccttcac gccaccggcg
    17221 gtcaaaccac tcttaataaa acgttccgaa agcgcataca taatcaccac cggcaatgcg
    17281 gtcaccagcg aggccgccat catccgtccc cagatgtaat caggcgtact gaacagcgcg
    17341 ttcaggccca ctggtaaggt gaaattgctg gcgctggaga ggaaaatcga cgcaaacaga
    17401 tagtcgttcc acgccaccat aaagcagtag acgaacaccg atatcagccc ggacatcgcc
    17461 aggggcacgg tgatacggaa gatgatttgc agacggttga ggccatccat catcgccgct
    17521 tcttcgatct catcagggat ggtgtcgaag taacttttca gcataaacac ggcggtgggc
    17581 agggtttgcg tcaccatggt aatgatcagc gccatctcgg tgtcgtaaat accaagcgcg
    17641 gtgatgattt tgaacagagg caccaccagc aaaatgccag agaacatata caccgtgtaa
    17701 aagctggcgt tgatggtcat ccggccttta aagcgcaggc gggaaagcgc gtaagctccg
    17761 agaatgccga gaaataccgc caccacggag gaaaccaccg acaccaccat gctgttacgg
    17821 aagtagtcga caaacgggaa aatcatcggg ttaaaaatgt cgacgtaatg ctccagcgtc
    17881 cactgttgcg gcagcagcgt aggatgcagc gatatcgcct ctttcgcgct tttgaacgag
    17941 gtcatcagca tcacaaaaaa cggaaacagc gtgatgatga gaaacagcgc cagcccgcag
    18001 taaaaaccga tacgactgag cgtgcgttta tttgttgcca ttgaggttca cccgtttcct
    18061 ggtcagcaga atgacagcga aaataatgat gaagagcacc accgagatcg ccgccgcttt
    18121 ccctaagtca ttaaaggcaa aggcggtttt gtagagatag acaccaagaa tatcgacctt
    18181 ggttgtcagc agataaacat cagcgaacat gtagaacatc cagatggtgc gcagggtcac
    18241 caccgtcgcc aggaccggca taattgcggg cagcgtgacg atgcgaaacc gctgccaggc
    18301 attagcgcca tccatctctg ccgcttcgta cagcgatttg tcgatggtct gcaaaatcgc
    18361 cagaaacgag ataaaggcat acgggaagta gcgccagatg gcgaacagca ccaccagcac
    18421 aaagctactg cccggattat caaaccacag cggtgcctgc tcatagagat gcagaagatc
    18481 gacgccgagg tagttcacaa tgccgtagcc gttgttgaac atgtatttcc aggcaaacac
    18541 cagcgaaatg gacggcgtta cgtaggagag gatcaccagc gaacgcgcgg ttttgcgcag
    18601 gcggaactcg cggttgaaaa acatcgccac ggccagcccg agcacggtgc tgcccgccac
    18661 caccagcgcc gtataccaga ccgtcatcca cagcgagtgc cagaagccgg gatcggagag
    18721 aatgcgcaca tagttactca ccccaacaaa cgttgactcg atgttgggat tgagcggcag
    18781 acgtaaaaaa ctgatttcga tattcgacac catcggccac gccaccagac cgcccagcag
    18841 taataagctg ggcgcgagaa gcagcagcgc aaacggcata tcggaacgac ctgaaaaaag
    18901 cctgttcatt gcggattcct gtcctgggtt tagtgctgtt cgatcagttc atccagcttt
    18961 ttctggctcg cttgcagcgt gccagggaga tcggctttac ccacggtgac gttatgcacc
    19021 atggagctca ccacaccaga acccgtcaca tcgcccatgc gggtaaagtt tttatcccct
    19081 actgcgccaa agacctgaat atttggcagt tcaccgatta gctgattcgg tagttcaccc
    19141 agcgccttaa taacgtcgtt gtctttccag gtggcggtag tgacaaccgc tttattcacc
    19201 ggcagcgcag cacctggcga catcatcacc cagtcggcaa tgttgtccgc ctgctccata
    19261 aaggtgacaa atttttctgc tgcttcggtc ttttgtccgg cggtaatggt cagcgaggtc
    19321 aacatgccgt agaccgcaga gtttttctcg gttggcacca cgaaaccgac gtttttcggg
    19381 tcgccttctt taatcacagc cggaaggata taggtggagt aaatcgccat cggcgcggtg
    19441 ccgttcataa aggcgtcttt cacttccatg atgtcgttag aacccggcat ggtgttggca
    19501 gcaaggtcgc ggtaataggt cagcgcctgc atcatctctg gtgtatcaag ggtgattttg
    19561 ccttcggcgt taaagacgtt ggcctagttg gataacgcaa actgggagaa ggattgttcc
    19621 gtcagcacgc tttctgccgt aggcagcgca ataccgtatt ttttattcgc cgggtcattc
    19681 agtttctgtg caacgtccag cagctgttgc cagtttttcg gctcctccag ccccgctttt
    19741 gccagcacat ctttgcgata ccagatgccg ccaatccagg cgctgacagg aacaccggtc
    19801 catgcgctac catcttcggt acgcacaata cgcagtacgc catcgtaaaa cgcgccttca
    19861 ccaacgttgc tgatgaccgt ggcaaccgct ttgcgatcga taagctgctc tttatccata
    19921 actttggcgt agtcatggct ggtttcgatc acttccggca gcgagccgct acgtgaaagg
    19981 gtgatgactt tggtgttatg agcgtcttct tccacgggca cttgtttaac ggtgatacct
    20041 gggttttctt tttcaaaacg ggcaatcaat ttgctgataa ctgcctgacg ctcctgctcc
    20101 accgaagaat gcataaattc gatggataca tgggttttat tttcttcttt acagcctgaa
    20161 atcagggcgc aggaaaccag tgctgataac agcacgattt ttgatttaat catgagatgt
    20221 tcctttttat ttaatccaca taacctgcca ggggcgcagc gttatttcat tacccaatat
    20281 atcaacttca ctaatcagat cccgaccatg cagatcagta atatttacat gctgaatatt
    20341 tttactgaca ttaaacaatc cagtcagaca attaccatca gcgttacttc tttgaatacg
    20401 cattacggat gaattaaccg tatcgatggt aaaattatta tccggatgaa actcgttatg
    20461 gctgcgacga agtgtaatta aacgcgacaa ctcatgatat accgcatgcc ttaatgtagc
    20521 ttcatcgttc agttctcgtg ttatctcttc actgtgatat tttttacggt taatcgcacg
    20581 gttatatccg agtttttcga cacctgcgta atcattacgc gagccaagaa tactttgaat
    20641 atatatcgcg ggtacaccgg ggaaacttaa caaaatcgca tgggcaagga taaacctggt
    20701 gcaacgttct tcatcgctac tctcgcggcg gcttaacgca ttcatatagg tgacattcat
    20761 ctcataggga ctgcgcgtgc cgtcgggatt atttttccag tttacta
//
LOCUS       DAECOD010000070        20712 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.070, whole genome shotgun sequence.
ACCESSION   DAECOD010000070 DAECOD010000000
VERSION     DAECOD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.070"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KKO15_002602"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KKO15_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBD6361343.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKVGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KKO15_002603"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KKO15_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBD6361344.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KKO15_002604"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KKO15_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBD6361345.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KKO15_002605"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KKO15_002605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBD6361346.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="KKO15_002606"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="KKO15_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBD6361347.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGHIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="KKO15_002607"
     CDS             complement(8217..9110)
                     /locus_tag="KKO15_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBD6361348.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="KKO15_002608"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="KKO15_002608"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBD6361349.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="KKO15_002609"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="KKO15_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBD6361350.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="KKO15_002610"
     CDS             complement(10241..11473)
                     /locus_tag="KKO15_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBD6361351.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="KKO15_002611"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="KKO15_002611"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBD6361352.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="KKO15_002612"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="KKO15_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBD6361353.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="KKO15_002613"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="KKO15_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="KKO15_002614"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="KKO15_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBD6361354.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="KKO15_002615"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="KKO15_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBD6361355.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="KKO15_002616"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="KKO15_002616"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBD6361356.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="KKO15_002617"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="KKO15_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBD6361357.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4983 a         5726 c         5031 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccac tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc acatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAECOD010000071        20699 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.071, whole genome shotgun sequence.
ACCESSION   DAECOD010000071 DAECOD010000000
VERSION     DAECOD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.071"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            81..350
                     /locus_tag="KKO15_002618"
     CDS             81..350
                     /locus_tag="KKO15_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBD6361358.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KKO15_002619"
     CDS             344..709
                     /locus_tag="KKO15_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBD6361359.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KKO15_002620"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KKO15_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBD6361360.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KKO15_002621"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KKO15_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBD6361361.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KKO15_002622"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KKO15_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBD6361362.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KKO15_002623"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KKO15_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD6361363.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KKO15_002624"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KKO15_002624"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBD6361364.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVSNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KKO15_002625"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KKO15_002625"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBD6361365.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="KKO15_002626"
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="KKO15_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBD6361366.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMEQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALYLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNQDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KKO15_002627"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KKO15_002627"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBD6361367.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KKO15_002628"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KKO15_002628"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBD6361368.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVSWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="KKO15_002629"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="KKO15_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBD6361369.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="KKO15_002630"
     CDS             complement(16437..17195)
                     /locus_tag="KKO15_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBD6361370.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KKO15_002631"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KKO15_002631"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBD6361371.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KKO15_002632"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KKO15_002632"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBD6361372.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="KKO15_002633"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="KKO15_002633"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBD6361373.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="KKO15_002634"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="KKO15_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4891 a         5692 c         5217 g         4899 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgagac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat atctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccagga cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct agtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAECOD010000072        20521 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.072, whole genome shotgun sequence.
ACCESSION   DAECOD010000072 DAECOD010000000
VERSION     DAECOD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.072"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KKO15_002635"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KKO15_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBD6361374.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KKO15_002636"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KKO15_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBD6361375.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KKO15_002637"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KKO15_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBD6361376.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KKO15_002638"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KKO15_002638"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBD6361377.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KKO15_002639"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KKO15_002639"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBD6361378.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KKO15_002640"
     CDS             4449..4814
                     /locus_tag="KKO15_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBD6361379.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTNWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KKO15_002641"
     CDS             4964..5482
                     /locus_tag="KKO15_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBD6361380.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KKO15_002642"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KKO15_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KKO15_002643"
     CDS             6713..7195
                     /locus_tag="KKO15_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBD6361381.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KKO15_002644"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KKO15_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBD6361382.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KKO15_002645"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KKO15_002645"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBD6361383.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KKO15_002646"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KKO15_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBD6361384.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KKO15_002647"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KKO15_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBD6361385.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KKO15_002648"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KKO15_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBD6361386.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KKO15_002649"
     CDS             10939..11730
                     /locus_tag="KKO15_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBD6361387.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KKO15_002650"
     CDS             11751..13037
                     /locus_tag="KKO15_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD6361388.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KKO15_002651"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KKO15_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBD6361389.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KKO15_002652"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KKO15_002652"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBD6361390.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KKO15_002653"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KKO15_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBD6361391.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KKO15_002654"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KKO15_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBD6361392.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KKO15_002655"
     CDS             complement(16985..17275)
                     /locus_tag="KKO15_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBD6361393.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KKO15_002656"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KKO15_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBD6361394.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KKO15_002657"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KKO15_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBD6361395.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KKO15_002658"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KKO15_002658"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KKO15_002659"
     CDS             19137..20378
                     /locus_tag="KKO15_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD6361396.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5360 c         4812 g         5047 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atgaggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac caactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatta
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAECOD010000073        20287 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.073, whole genome shotgun sequence.
ACCESSION   DAECOD010000073 DAECOD010000000
VERSION     DAECOD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.073"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="KKO15_002660"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="KKO15_002660"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBD6361397.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="KKO15_002661"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="KKO15_002661"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBD6361398.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="KKO15_002662"
     CDS             complement(1728..2999)
                     /locus_tag="KKO15_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361399.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="KKO15_002663"
     CDS             complement(3182..4387)
                     /locus_tag="KKO15_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361400.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAEHFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="KKO15_002664"
     CDS             complement(4487..4843)
                     /locus_tag="KKO15_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBD6361401.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="KKO15_002665"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KKO15_002665"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="KKO15_002666"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="KKO15_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBD6361402.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="KKO15_002667"
     CDS             6773..9829
                     /locus_tag="KKO15_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD6361403.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="KKO15_002668"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="KKO15_002668"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBD6361404.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="KKO15_002669"
     CDS             10336..10524
                     /locus_tag="KKO15_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBD6361405.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="KKO15_002670"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="KKO15_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBD6361406.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="KKO15_002671"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="KKO15_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBD6361407.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="KKO15_002672"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="KKO15_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBD6361408.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="KKO15_002673"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="KKO15_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBD6361409.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="KKO15_002674"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="KKO15_002674"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBD6361410.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="KKO15_002675"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="KKO15_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBD6361411.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="KKO15_002676"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="KKO15_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBD6361412.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="KKO15_002677"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="KKO15_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBD6361413.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="KKO15_002678"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="KKO15_002678"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBD6361414.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5314 a         4860 c         5177 g         4936 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaatgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gactgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctga
//
LOCUS       DAECOD010000074        20231 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.074, whole genome shotgun sequence.
ACCESSION   DAECOD010000074 DAECOD010000000
VERSION     DAECOD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.074"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(435..641)
                     /locus_tag="KKO15_002679"
     CDS             complement(435..641)
                     /locus_tag="KKO15_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBD6361415.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="KKO15_002680"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="KKO15_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBD6361416.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="KKO15_002681"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="KKO15_002681"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBD6361417.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="KKO15_002682"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="KKO15_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="KKO15_002683"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="KKO15_002683"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBD6361418.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="KKO15_002684"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="KKO15_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="KKO15_002685"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="KKO15_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBD6361419.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="KKO15_002686"
     CDS             8989..9093
                     /locus_tag="KKO15_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBD6361420.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="KKO15_002687"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="KKO15_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="KKO15_002688"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="KKO15_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBD6361421.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="KKO15_002689"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="KKO15_002689"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBD6361422.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="KKO15_002690"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="KKO15_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBD6361423.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="KKO15_002691"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="KKO15_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6361424.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="KKO15_002692"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="KKO15_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBD6361425.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="KKO15_002693"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="KKO15_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBD6361426.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="KKO15_002694"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="KKO15_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBD6361427.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="KKO15_002695"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="KKO15_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBD6361428.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="KKO15_002696"
     CDS             complement(18055..18381)
                     /locus_tag="KKO15_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361429.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="KKO15_002697"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="KKO15_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBD6361430.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAISASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4701 a         5042 c         5350 g         5138 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggttgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttactcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacgatac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 ggggggtcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgctg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt atgtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg a
//
LOCUS       DAECOD010000075        19741 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.075, whole genome shotgun sequence.
ACCESSION   DAECOD010000075 DAECOD010000000
VERSION     DAECOD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.075"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            15..74
                     /gene="ibsE"
                     /locus_tag="KKO15_002698"
     CDS             15..74
                     /gene="ibsE"
                     /locus_tag="KKO15_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBD6361431.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(194..1627)
                     /gene="hldE"
                     /locus_tag="KKO15_002699"
     CDS             complement(194..1627)
                     /gene="hldE"
                     /locus_tag="KKO15_002699"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBD6361432.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1675..4515)
                     /gene="glnE"
                     /locus_tag="KKO15_002700"
     CDS             complement(1675..4515)
                     /gene="glnE"
                     /locus_tag="KKO15_002700"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBD6361433.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4538..5839)
                     /gene="ygiF"
                     /locus_tag="KKO15_002701"
     CDS             complement(4538..5839)
                     /gene="ygiF"
                     /locus_tag="KKO15_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBD6361434.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6081..6701
                     /locus_tag="KKO15_002702"
     CDS             6081..6701
                     /locus_tag="KKO15_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBD6361435.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6765..8003
                     /gene="cca"
                     /locus_tag="KKO15_002703"
     CDS             6765..8003
                     /gene="cca"
                     /locus_tag="KKO15_002703"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBD6361436.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8166..8987)
                     /gene="bacA"
                     /locus_tag="KKO15_002704"
     CDS             complement(8166..8987)
                     /gene="bacA"
                     /locus_tag="KKO15_002704"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD6361437.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9078..9446)
                     /gene="folB"
                     /locus_tag="KKO15_002705"
     CDS             complement(9078..9446)
                     /gene="folB"
                     /locus_tag="KKO15_002705"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBD6361438.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGACFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9551..10168
                     /gene="plsY"
                     /locus_tag="KKO15_002706"
     CDS             9551..10168
                     /gene="plsY"
                     /locus_tag="KKO15_002706"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBD6361439.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10181..11112)
                     /gene="ttdR"
                     /locus_tag="KKO15_002707"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10181..11112)
                     /gene="ttdR"
                     /locus_tag="KKO15_002707"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11319..12162
                     /gene="ttdA"
                     /locus_tag="KKO15_002708"
                     /pseudo
     CDS             11319..12162
                     /gene="ttdA"
                     /locus_tag="KKO15_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12159..12764
                     /gene="ttdB"
                     /locus_tag="KKO15_002709"
     CDS             12159..12764
                     /gene="ttdB"
                     /locus_tag="KKO15_002709"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBD6361440.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12812..14275
                     /gene="ttdT"
                     /locus_tag="KKO15_002710"
     CDS             12812..14275
                     /gene="ttdT"
                     /locus_tag="KKO15_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBD6361441.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14318..15331)
                     /gene="tsaD"
                     /locus_tag="KKO15_002711"
     CDS             complement(14318..15331)
                     /gene="tsaD"
                     /locus_tag="KKO15_002711"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBD6361442.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15569..15784
                     /gene="rpsU"
                     /locus_tag="KKO15_002712"
     CDS             15569..15784
                     /gene="rpsU"
                     /locus_tag="KKO15_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBD6361443.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15895..17640
                     /gene="dnaG"
                     /locus_tag="KKO15_002713"
     CDS             15895..17640
                     /gene="dnaG"
                     /locus_tag="KKO15_002713"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD6361444.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17835..19676
                     /gene="rpoD"
                     /locus_tag="KKO15_002714"
     CDS             17835..19676
                     /gene="rpoD"
                     /locus_tag="KKO15_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBD6361445.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4694 a         5510 c         5046 g         4491 t
ORIGIN      
        1 agaaggaaag ggttatgatg aagttcgtca tcatactggt tgtgttgtta ctgttaagtt
       61 tcccgactta ctaacaactg atcagagggg ggagaaatcc tcccttaccc ttgttccttt
      121 actttaggtt gaaaaaacaa cagcgtcaat aggcctgcct gctacgaagc gagatctgtg
      181 aaccgctttc cggttagcct tttttatcct gttggatctt cttgatgatg ttagtggtcg
      241 agcaaccgtc ttcgaagttg agcaccagca cttcgccgcc gttggcccag acttctttac
      301 tcccggcgat ctcttctggt ttatagtcgc cgcccttcac cagtaaatct ggcaggatcc
      361 cggcgatcaa gcgctgcggc gtgtcttctt caaacgacac cacccagtcg accgcttcca
      421 gtgcgcccag cacaatcata cgctgttcga gcgggttaac ggggcgagaa tcccctttca
      481 gccgtttggt ggaggcatcg ctgttcacag cgacaatcaa gcggtcaccc agtttgcggg
      541 catttgccag atagaagacg tgtccggcgt gcaggatgtc aaagacgcca ttggtcatca
      601 ccactttttc accacgttta cgcgctgccg ctacggccag cttcagttcc tcttcggtca
      661 tcacgccaaa gccggtatct gcgcgcccac gcactgcatt ttccagctcg atcggcgaaa
      721 ccgtggaggt tcccagtttg ccgaccacca cgccagccgc cgcattggca aagaagcagg
      781 cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc accgtgtcgc
      841 ccgcaccggt aacgtcatac acttcctgcg cctgggttgg catatgcagc ggcgctttac
      901 ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc gagagttcgt
      961 aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta catttaccga
     1021 caacagcttc aaattccgag agattcggtg tcagcagcgt agcgccgcgg tagcgctcaa
     1081 aatcggtacc ttttggatca atcagcaccg gaacgcccgc tttacgcgcc agttggatca
     1141 tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc gcgccaatcg
     1201 aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacacct tcgaaacctt
     1261 cttcaaaatc cagacggatc agctgttggt tgcgggaaag tacccgtaat ttggtgatgg
     1321 tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc agagatttac
     1381 tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac caggcgtgca ttagcaccga
     1441 gagaagcgat attcatcgcc acgttagccg cgccgcccgg acgttcttcg atggtattca
     1501 ctttaaccac gggcaccggc gcttccggcg agatacgact ggtggggccg taccagtaac
     1561 gatccagcat cacatcacca accaccatca ctcctgcacg ttcaaactct ggcagcgtta
     1621 ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca tacttcattc
     1681 ttccaccagc cacttctgcc agcttgcccg caccagatca cgctctgcgg tgaagcaatc
     1741 ctccgacaca tggcccggca attcctgtaa tgccagatga tgaagttcat cgcgcagcgt
     1801 cgtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt tttgcgccag
     1861 cagctccaga atacgcacgt tgtctgacca gcgcgttaac ttcggttttt catgagcgta
     1921 gcgcaacacc agatattggg taataaattc gatatcggta attccgcctt catcggcttt
     1981 gatatcaaag cgattacgat gtttattgcc gagatgagcg cgcattttct cgcgcatttc
     2041 ccgcacttcc gtttgcagag ttttaccttc tcgcggcagt gtcataatct cacgacgcac
     2101 cgcgtcaaag tgcgcagtga gctgcggatc gccgtacact acacgcgcac gcaccagcgc
     2161 ctgatgttcc cacgtccagg cctcgttttt ctgataatcg gcaaatgctt ctgcggatgt
     2221 caccagcatt cccgccgccc cggacggacg cagtcgagca tccacttcat acaaaatgcc
     2281 ggaagaggta cgcgtactga acagatgcat aatgcgttgc gccagacgca gataaaactg
     2341 ccgcccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat catggaggaa
     2401 gataaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc cgtagccgac
     2461 caccgcaaaa ccgcgccctt cacgttcgtt gaggtgattt ggcttaccgt agcgggccac
     2521 catttgaccc cacgcctgct gaacgacggc atctatcata gcttctgcca gccaggttaa
     2581 gtgatcgctc actttcatta ccggtagcgt accggcgata tccgctgccg caatgcgtaa
     2641 cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgttcttcgt catcttccgg
     2701 cacgcgcagc aaatactggc gcagttcatc acggtaggca tcggtcgccg tcggctgata
     2761 aagggtattt ggatcgagca attcatccag caataatgga taacgcgcta gctggctggc
     2821 aatcatcggc gatgcggcac acagagaaat caaatgttta agcgccgcag ggaattcact
     2881 gagcaattct aaataggttg tgcgggtaac aatccccacc agcaaggcgg taatgcgcga
     2941 cagcgtgaca gcagcgtctt cacgcgcaca gacatcactt agcagatgtg gcatcagatg
     3001 gtcgagcacc tgacgtcctc gcggcccgat agtacgctta tccagctctt tgcggaaatc
     3061 agcaatcagc gttaggacct gtttgcgatc atcctcgcta agatgcgcca gcactggcgt
     3121 agtgtcatct tcctgcaacg catcctgcca cagctcacgc cactgttccg acagcgactc
     3181 ttcctgagtt tcactttcgt cgtcgccaat caattcatta aacacccggc gcacattggt
     3241 catatgtgcg gtcagcgccc cggtcagttg cggccagtca gcaaagtcca tcgcccacgc
     3301 cagccgcgca cggttaagct catcagaagg aagcgtttgg gtttgttcgt cgttaatgct
     3361 ttgcagcagg ttttccagac gccgcaggaa cagatatgcc actcgcaatt gttcagcatc
     3421 gttttcagaa agcagatgca gcgcggcaat ggcgctgagc gttggcagta aagagcgcga
     3481 ctgcagcaac ggttcgcgcc caccgcgaat gagctggaac acctgaacga taaattcaat
     3541 ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc gacgcacttc
     3601 acgggcaatc atccctttca tgttgcgcag cgactgaatc acgctgaaat cgatgtaacg
     3661 acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc cttcgctatc
     3721 gcccataatc cgcgctttga ccatcgcgta acgctcccag tcgcgtccct gctcctggta
     3781 ataatcctcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa acggacgcag
     3841 tcgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccagca ctttaatcag
     3901 ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc cgccctgcgt
     3961 acaaccatgt tccggccagg caaaaatcag atcgatatca gaggagaaat tcagttcccc
     4021 gccgcccagc ttacccatgc ctaagatcag cagcggttgc gcttcgccct gcgcattgca
     4081 cggcgttccc cactcgcggc agcaggcgtc atacagccag tcacgcgccg caacaatcag
     4141 cgtctccgcc agataactga gctgctgcaa tatgctctct tcagtaacca gtgccagcgt
     4201 ttgcgcccat gcgatgcgca ccataatgcg ccgccggaat agccgcagct cgcgcattaa
     4261 cccggcttcg tcgctcacat tactgagcgc ctcctgcaac cacacgctgt aatgttgcca
     4321 ttcgtcagcc tgtggcggtt ggctttccag ttccgtcagc cactctggat gcgcaatcac
     4381 gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcac taagtgattc
     4441 ctcggctaaa agctctggca gccgctcaac aacggtctgc cagtactgct gtaacggtga
     4501 agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg ctgtgcaacc
     4561 agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcaatg cgttgcccgg
     4621 tcgcaatagc gtgacgcagc ccctgccagt tctccagcca ggcttgcgcg acgacaggat
     4681 catagtaacc cgccagcaac agtattgagt caatatcacg catcaggcgt ggcaactggt
     4741 cacggtagcg atcgcctaac ggctggcaga aaacgctttt cagttcagcg gcatggcggg
     4801 aaagatggat atcggcaaag cgtttgaagg agtcgctaat tttgccctgc gctttggcat
     4861 ctaaaaacgg ctgccatgct ttgcttacca gccattcggt caacgccagc ttcgccattg
     4921 ccgtttcggt agagtagacc gccgtcacgg cagaaaccgc agaagcgatg gtcgcttcgc
     4981 attgagtcag caaatcacgt aagtgagtac tcgctttacg cggcacaatg ccaccgaaca
     5041 gcatcagtgt atgacggact aggctaatgg ctgccagcac ctgctctttc gccgcatcgt
     5101 tgccgcgtac ccacagttct tcatgatact gccattgcgc taacaccagc tcgagcgccg
     5161 cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg gtcggtttga
     5221 tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg ctcaggctgc
     5281 cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc gcgcgcgtgt
     5341 cgccgctaag cagctccagt tccagctcgc agataggctc agcaaattca cccgctttca
     5401 cttccccctg gtcgagagcg atttcaattc ggctgccatc gatctctacc agccattttt
     5461 cgcgataaaa atcggtgcta aacagcgact gcacgcggga agcgagatcg gcaggcaatt
     5521 cgccgttcgg ccagatttcc gtcggtaact gcgccaggtc gagcgtcggt tcgctcaacg
     5581 ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca actttcatgg
     5641 tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc ctacgcagcc
     5701 agttatccgg cgtttcgtag taaatattca gcaactgcac ggggtcatgg tgctcgccgc
     5761 ccagcgtatt gagatggtca cgcaacgcct caacggcact gtgattaaca ataaacttta
     5821 attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt gacgatgaac
     5881 ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa ttgccatgca
     5941 acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca cagaggattc
     6001 cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt tccactttcc
     6061 atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact ttactcgcac
     6121 ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta tgtttctgac gaactgaata
     6181 cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt aacgccggcg
     6241 aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg aaagacagct
     6301 ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc ctgcgctccc
     6361 gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc aatatcgata
     6421 acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc gacagcgtga
     6481 tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga gctgattgtc gcgcagaaaa
     6541 aggtcgatgc cgccagcgta cagctggatg acaaacagcg caccatcatc atgcaatggt
     6601 ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct cggtctggta ctgccgcacc
     6661 tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta aatcgccttc ttcgttgcac
     6721 tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag atttatctgg
     6781 tcggtggtgc tgttcgggat gcattgttag gactatcggt caaagacaga gattgggtgg
     6841 tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta ggccgcgatt
     6901 ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt accgaacgga
     6961 aatccggttc cggttacacc ggttttactt gctatgccgc accggatgtc acgctggaag
     7021 atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat aacggcgaga
     7081 ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc catgtttccc
     7141 ccgcttttgg cgaagatccg ttacgcgtac tgcgcgtggc gcgttttgct gcgcgttatg
     7201 cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag atgacccatg
     7261 cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacggaa agcgccctca
     7321 ccacccgcaa tccccaggtg ttcttccagg tactgcgcga ttgcggcgca cttcgcgttt
     7381 tattcccgga aattgacgca ctgtttggcg ttccggcacc tgccaggtgg catccggaaa
     7441 tcgacacggg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg agtccgcagg
     7501 ttgatgtccg tttcgcaact ttatgccacg atctcggtaa aggactgacg ccgccagaac
     7561 tttggccgcg tcatcatggg catggcccgg cgggtgttaa gttagtggaa caactatgcc
     7621 agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct gagtttcacg
     7681 atctcatccg caccttccca atgctgaacc caaaaaccat cgtcaaatta tttgattcca
     7741 tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc gaggctgacg
     7801 tgcgcggcag aaccggtttt gaatcagcgg actacccgca aggccgttgg ttgcgcgaag
     7861 gctgggaagt ggcgcagtca gtgccgacaa aagcggtcgt tgaagcggga tttaaaggcg
     7921 tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagc tggaaggaac
     7981 aacgttgccc aaagcctgac tgagtataaa actaaacgtt agcacgaacc gtcccctcgc
     8041 cccttcgggg agagggttag ggtgagggga acagaccagc accggtgcga acaatcaccc
     8101 ccaccccggc cctctcccta cagagcgagg gagaaaaccc ggcctgctcc cgaacctttt
     8161 ctcaatcaaa agaacacgac atacaccgca gccgccacaa taaagcgata aatggcgaac
     8221 gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat cagcgccacc
     8281 acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggttgt caggaagccc
     8341 cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggtaccgc cagcaggaac
     8401 gagaactcgg aagcagcgta acggctcacg cccatcagca tcccacctga aatggtcgcc
     8461 ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat cataaacgcc
     8521 tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt caggcattcg
     8581 gcggcaatca gcaacaaacc gccaacgacc agcgcataca tcacatttat cgggttaaac
     8641 aatgacttaa tcgtgtcgtg gaataatagc cccagtacca ccgccggaat catccccagc
     8701 aaaatgtgga tcagcgttaa acgacctttg ctttcacctt cgtgctgcaa cgggcggcca
     8761 aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac cgccagaatt
     8821 gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag
     8881 tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt caatccttcg
     8941 accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa taaaccccta
     9001 aattattaaa atgtaccgct tgtccgaact actgcagtat gaccaggtta taaccgtttg
     9061 gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg
     9121 ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc
     9181 gagttgaagc gtgctaacag cagctccgcc acctcttcag ccacgcgttc caccagcgca
     9241 aaacacgccc cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta actgaggcaa
     9301 tccgccacat catcactttt cgccgcttta cggttatccc atgccatttc gatatcgaac
     9361 actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa
     9421 agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta accggatacc
     9481 acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa ttttcaaaac
     9541 ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc
     9601 catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc gaaccagcgg
     9661 ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc
     9721 agtactgatt ttcgacgttc tgaaaggaat gttgcctgtc tggggcgcgt atgaattagg
     9781 tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac acatctggcc
     9841 cgttttcttc ggttttaaag gaggaaaagg cgttgctacc gcttttggag caatcgcacc
     9901 aattagctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg tgctattgag
     9961 cggatactcg tcgctgggag cgattgtcag tgcactgatt gctccgtttt atgtctggtg
    10021 gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc tgctgcgtca
    10081 tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa
    10141 aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat ctacatgacc tgatagcctt
    10201 catcgggctt gcccagccgt tgctggcacc atgccgccag aaattccgcg cagacacgaa
    10261 tttcatgctg cgatagagca gctcccggta aacagcccag atattggcgc tttgtgcata
    10321 ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat cccactcgga
    10381 acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc cgctattgga
    10441 ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca actcccatat
    10501 tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta attcctgtaa
    10561 tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata ttcttttatt
    10621 tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac gaatatccag
    10681 atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat gcacctgtaa
    10741 ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc gcccaaaacc
    10801 aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg tgacgtcatc
    10861 gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac gttggccact
    10921 ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca gcgtattttc
    10981 aaggatttgg atccgcttgg tgacaaatgc aggcgtttgc ccaagcgtcg ccgctgcagc
    11041 actaaaactc ccgctgtgga cgatttctac cagtacctgc aagtctttgg ctaaaggcca
    11101 gctattcagc atcgttaatt atccgcagtt gtgataagcg cagtgtattt cgcaaaacat
    11161 tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc acgaactgtt
    11221 aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtgttg gtttgtggcg
    11281 gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa agtaataagc
    11341 aacaggcagt gaataagttg acagagattg tcgctaactt taccgccatg atttctaccc
    11401 gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa acgtcgtcga
    11461 tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt gacctgaatc
    11521 gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt tcccgcttcc
    11581 cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca accgtcaaag
    11641 cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc aaaaataccg
    11701 gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat gcggaaatcg
    11761 aagtttacat ggcaggcggc ggctgcacgc tacctggccg ctcgaaagtg ttaatgccgt
    11821 cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc ctcgccgtaa
    11881 acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa accgccgccg
    11941 tactctcgcg taaagccatt ttgcgcccga ttggcagccg ccatcccaat ccaaaagcgg
    12001 cagaactgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt ccacaagggc
    12061 tgaccggcaa cagttcagtg atgggcgcat cgtcgcggca cgctgctggt tcatgccgat
    12121 ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc ctgacaaccc
    12181 cgatcaaagc tgaagatctg caagatattc gcgtcggcga tgtgatctac ctgaccggta
    12241 cgctggtgac ctgccgcgac gtttgtcacc gccgtttgat cgaactgaaa cgtccgatcc
    12301 cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc aaaaacggtg
    12361 acaaatggga gatggtctcc gtcggcccga caaccagtat gcgtatggaa agttttgaac
    12421 gtgaatttat tgagcagacc ggcgtaaaac tggtggttgg caaaggtggt atggggccgc
    12481 tgaccgaaga aggctgccag aaattcaagg cgttacatgt gattttccca gcaggctgcg
    12541 cggtgctggc ggcaacccag gtagaagaga ttgaagaagt gcactggaca gaactcggga
    12601 tgccggagtc actgtgggtc tgccgggtca aagagttcgg cccgctgatt gtctctattg
    12661 atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa cgccgcgatc
    12721 ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc cggagaggct
    12781 cacccctctc ctttttcgca ggcataacac gatgaaacct tccactgaat ggtggcgata
    12841 tcttgcgccg ctggcggtca tcgccattat tgctctactt ccggttcccg cagggctgga
    12901 gaaccatacc tggctctact ttgccgtttt tactggcgtg atcgttggac tgatcctcga
    12961 acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc atcatcgcca tactctctcc
    13021 ctggctgctg ttcagccccg aacagctcgc ccagccaggc tttaagttca ccgccaaatc
    13081 cctctcctgg gcagtttctg gtttttctaa ttcggttatc tggctcattt tcgccgcctt
    13141 tatgtttggc acaggctatg aaaaaaccgg gcttggacgc cgcattgcgc tgattctggt
    13201 gaaaaagatg gggcatcgca cgctgtttct cggctatgcg gtgatgttct ccgagctgat
    13261 cctggcacct gtaacaccgt ccaactcggc gcgtggtgcg ggtattatct atcccatcat
    13321 ccgtaacctg ccaccgctct atcaatcaca accaaacgac agcagttcgc gcagcattgg
    13381 ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg accagcgcca ttttcctgac
    13441 ggcgatggca cctaacttgc tgttaattgg actgatgaaa agcgcatctc acgccacgct
    13501 gagttggggc gactggttcc tcgggatgtt gccgctcagc attttactgg ttctgctggt
    13561 tccctggctg gcttacgtgc tgtacccgcc ggtactgaag tctggtgatc aggtgccgcg
    13621 ctgggcagag acggaactgc aggcaatggg cccgctctgt tcgcgtgaaa aacggatgct
    13681 ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc ggtgattata tcgatgccgc
    13741 gatggtcggt tacagcgtgg tggcgctgat gctgcttctg cgcattatca gttgggacga
    13801 cattgtcagt aataaagcgg catggaacgt tttcttctgg ctggcctcgc ttatcaccct
    13861 cgcgaccgga ctcaacaata ccggttttat tagctggttt ggcaaactgt tagcaggcag
    13921 cttaagtggt tattcgccga cgatggtgat ggtgacgttg attgtggtgt tttatctact
    13981 gcgctacttt ttcgccagcg ccacggcgta tacctgcgcc ctcgcaccga tgatgatcgc
    14041 cgccgcgctg gcgatgccgg aaatcccgct gccggtgttc tgcctgatgg ttggcgcggc
    14101 aattggtctg gggagcattc ttacaccata cgccaccgga cccagtccga tttactacgg
    14161 tagtggttat ctgccaacgg cggattactg gcgactgggg gcgatttttg ggctgatatt
    14221 cctcgtgttg ctggtgatta ccggcttact gtggatgccc gtggtgttgc tttaagtgag
    14281 gacggtgccg gaaattgctc tccggcaccc aatagtttta cgcagccggt aactccgcca
    14341 gcggccagcg cggacgcacg ctaacgccga gatccgccgt cgcgcctgct ttaaaccgca
    14401 ccattccggc ataggcgatc atcgcgccgt tatcagtaca aaattccgga cgcgcgtaga
    14461 acacttcgcc gcggcgtttt ttcatcattt cagccagctt cgcccgtaac gtgcggttag
    14521 cactcacgcc gcccgccatg accagtcgct taaagcccgt ctgatccaac gctcgcttac
    14581 acttaatcat caacgtatcg accaccgcat cttcaaaggc gcgggcgata tcagcacgcg
    14641 tctggtcgtc ggtgccgttg tcacgaatcg tgttcgccgc aaaggttttc agaccagaaa
    14701 agctgaaatc cagccccgga cggtcggtca tcggacgcgg gaaaacaaag cgcccggcag
    14761 taccctgagc cgccattttc gacagtaacg gtccgccagg ataatccagc cccagcagct
    14821 tcgcggtttt atcaaacgct tcaccggcgg catcatcgat agactcgccg agcagctcgt
    14881 actgaccaat gccagtcacg ctgattaact gcgtatgacc gccggacacc agcagcgcga
    14941 caaacggaaa ttccggcggg ttatcttcca gcatcggcgc taacagatgc ccttccatat
    15001 ggtgtaccgg gattgccgga acgttccagg caaacgccag agaacgcccc acggttgcgc
    15061 caaccagcag tgcgccgact aatccagggc ctgcggtata ggccacagca tcaatgtctt
    15121 ttgccgttaa accagactcc tttagcgccg cctggatcaa cggtacggtt ttacgcacat
    15181 gatcgcggga ggccagttca ggcacgacgc cgccgtagtc agcgtgcaat ttcacctgac
    15241 tatacaattg gttggctaac aaaccttttt catcgtcgta aatggcgatg ccggtttcat
    15301 cgcaggaagt ttcaataccc agtacacgca tgacttgttt tacctcgctt tattaccgcg
    15361 cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg cccctggaga aagcctcgtg
    15421 tatactcctc acccttataa aagtcccttt caaaaaaggc cgcggtgctt tacaaagcag
    15481 cagcaattgc agtaaaattc cgcaccattt tgaaataagc tggcgttgat gccagcggca
    15541 aaccgaatta atcaaaggtg agaggcacat gccggtaatt aaagtacgtg aaaacgagcc
    15601 gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg ttctggcgga
    15661 agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg ctaaagcttc
    15721 tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca ctcgtctgta
    15781 ctaattcccc gagagcgttg ctctccgatc agaccgagtt gtagttgtaa ggccgtgctt
    15841 ccgaaaggaa tgcgcggctt attttcgttt atgaattgct aaaaatcggg gcctatggct
    15901 ggacgaatcc cacgcgtatt cattaatgat ctgctggcac gcactgacat cgtcgatctg
    15961 atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg ttgtccattc
    16021 cacaacgaga aaaccccgtc attcaccgtt aacggtgaga aacagtttta ccactgcttt
    16081 ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga actacgacaa gctcgagttc
    16141 gtcgaaacgg tcgaagagct ggcagcaatg cacaatcttg aagtgccatt tgaagcaggc
    16201 agcggcccca gccagatcga gcgccatcag aggcaaacgc tttatcagtt gatggacggt
    16261 ctgaatacgt tttaccaaca atctttacaa caacctgttg ccacgtctgc gcgccagtat
    16321 ctggaaaaac gcggattaag ccacgaggtt atcgctcgct ttgcgattgg ttttgcgccc
    16381 cccggctggg acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg ccagtcattg
    16441 attgatgcgg ggatgttggt cactaacgat cagggacgca gttacgatcg tttccgcgaa
    16501 cgggtgatgt tccccattcg cgataaacgc ggtcgggtga ttggttttgg cgggcgcgtg
    16561 ctgggcaacg atacccccaa atacctgaac tcgccggaaa cagacatttt ccataaaggc
    16621 cgccagcttt acggtcttta tgaagcgcag caggataacg ctgaacccaa tcgtctgctt
    16681 gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat acggcattaa ttacgccgtt
    16741 gcgtcgttag gtacgtcaac caccgccgat cacatacaac tgttgttccg cgcgaccaac
    16801 aatgtcattt gctgttatga cggcgaccgt gcaggccgcg atgccgcatg gcgagcgctg
    16861 gaaacggcgc tgccttacat gacagacggc cgtcagctac gctttatgtt tttgccggat
    16921 ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag aagcgtttga agcgcggatg
    16981 gagcaggcga tgccactctc cgcatttctg tttaacagtc tgatgccgca agttgatctg
    17041 agtacccctg acgggcgcgc acgtttgagt acgctggcac tgccattgat atcgcaagtg
    17101 ccgggcgaaa cgctgcgaat atatcttcgt caggaattag gcaacaaatt aggcatactt
    17161 gatgacagcc agcttgaacg attaatgcca aaagcggcag agagcggcgt ttctcgccct
    17221 gttccgcagc taaaacgcac gaccatgcgt atacttatag ggttgctggt gcaaaatcca
    17281 gaattagcga cgttggtccc gccgcttgag aatctggatg aaaataagct ccctggactt
    17341 ggcttattca gagaactggt caacacttgt ctctcccagc caggtctgac caccgggcaa
    17401 cttttagagc actatcgtgg tacaaataat gctgccaccc ttgaaaaact gtcgatgtgg
    17461 gacgatatag cagataagaa tattgctgag caaaccttca ccgactcact caaccatatg
    17521 tttgattcgc tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg cacgcatggt
    17581 ttaagcaacg aagaacgcct ggagctctgg acattaaacc aggagctggc gaaaaagtga
    17641 tttaacggct taagtgccga agagcgatcg ggaagccccc gacagccgca ctgagaggca
    17701 gcggcaaata tataagtacg ccctcgtaat tatcgttggc ggtaaacaac cgttggattt
    17761 cagcgttaac ggctgaagga catcgggtca atcgcccaac accaacctca tgaaataagt
    17821 gtggataccg tcttatggag caaaacccgc agtcacagct gaaacttctt gtcacccgtg
    17881 gtaaggagca aggctatctg acctatgccg aggtcaatga ccatctgccg gaagatatcg
    17941 tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc attcaggtga
    18001 tggaagaagc accggatgcc gatgatctga tgctggctga aaacaccgcg gacgaagatg
    18061 ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg cgcacgactg
    18121 acccggtacg catgtacatg cgtgaaatgg gcaccgttga actgttgacc cgcgaaggcg
    18181 aaattgacat cgctaagcgt attgaagacg ggatcaacca ggttcaatgc tccgttgctg
    18241 aatatccgga agcgatcacc tatctgctgg aacagtacga tcgtgttgaa gcagaagaag
    18301 cgcgtctgtc cgatctgatc accggctttg ttgacccgaa cgcagaagaa gatctggcac
    18361 ctaccgccac tcacgtcggt tctgagcttt cccaggaaga tctggacgat gacgaagatg
    18421 aagacgaaga agatggcgat gacgacagcg ccgatgatga caacagcatc gacccggaac
    18481 tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt tgtaacgcgt gacaccatca
    18541 aagcgaaagg tcgcagtcac gctgccgctc aggaagagat cctgaaactg tctgaagtat
    18601 tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc atgcgcgtca
    18661 tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt gagcagtgca
    18721 aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc gatacctggt
    18781 tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa actgcacgat gtctctgaag
    18841 aagtgcatcg cgccctgcaa aaactgcagc agattgaaga agaaaccggc ctgaccatcg
    18901 agcaggttaa agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc cgccgtgcga
    18961 agaaagagat ggttgaagcg aacttacgtc tggttatttc tatcgccaag aaatacacca
    19021 accgtggctt gcagttcctt gacctgattc aggaaggtaa catcggcctg atgaaagcgg
    19081 ttgataaatt cgaataccgc cgtggttaca agttctccac ctacgcaacc tggtggatcc
    19141 gtcaggcgat cacccgttct atcgcggatc aggcgcgcac catccgtatt ccggtgcata
    19201 tgattgagac tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa gagatgggcc
    19261 gtgaaccgac gccggaagaa ctggctgaac gtatgctgat gccggaagac aagatccgca
    19321 aagtgctgaa gatcgccaaa gagccaatct ccatggaaac gccgatcggt gatgatgaag
    19381 attcgcatct gggggatttc atcgaggata ccaccctcga gctgccgctg gattctgcga
    19441 ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc gcgcgtgaag
    19501 caaaagttct gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg ctggaagaag
    19561 tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg aaggcgctgc
    19621 gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag cttcctggac gattaatcgg
    19681 taggccggat caggcgttac gccgcacccg gcactagtct ctctgcacaa acgccacctt
    19741 t
//
LOCUS       DAECOD010000076        19711 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.076, whole genome shotgun sequence.
ACCESSION   DAECOD010000076 DAECOD010000000
VERSION     DAECOD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.076"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..358
                     /locus_tag="KKO15_002715"
     CDS             <1..358
                     /locus_tag="KKO15_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD6361446.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="KKO15_002716"
     CDS             complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="KKO15_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBD6361447.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVTDA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="KKO15_002717"
     CDS             complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="KKO15_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBD6361448.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3113..3265
                     /gene="mokC"
                     /locus_tag="KKO15_002718"
     CDS             3113..3265
                     /gene="mokC"
                     /locus_tag="KKO15_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBD6361449.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="KKO15_002719"
     CDS             complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="KKO15_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBD6361450.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="KKO15_002720"
     CDS             complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="KKO15_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBD6361451.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6881..7285
                     /locus_tag="KKO15_002721"
     CDS             6881..7285
                     /locus_tag="KKO15_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBD6361452.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7311..8024
                     /gene="msyB"
                     /locus_tag="KKO15_002722"
     CDS             7311..8024
                     /gene="msyB"
                     /locus_tag="KKO15_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBD6361453.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8173..8739
                     /gene="satP"
                     /locus_tag="KKO15_002723"
     CDS             8173..8739
                     /gene="satP"
                     /locus_tag="KKO15_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBD6361454.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8774..9361)
                     /gene="mog"
                     /locus_tag="KKO15_002724"
     CDS             complement(8774..9361)
                     /gene="mog"
                     /locus_tag="KKO15_002724"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBD6361455.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9476..10429)
                     /gene="tal"
                     /locus_tag="KKO15_002725"
     CDS             complement(9476..10429)
                     /gene="tal"
                     /locus_tag="KKO15_002725"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD6361456.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10708..12138
                     /locus_tag="KKO15_002726"
                     /pseudo
     CDS             10708..12138
                     /locus_tag="KKO15_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12208..12984
                     /gene="yaaA"
                     /locus_tag="KKO15_002727"
     CDS             12208..12984
                     /gene="yaaA"
                     /locus_tag="KKO15_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBD6361457.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13137..13433)
                     /locus_tag="KKO15_002728"
     CDS             complement(13137..13433)
                     /locus_tag="KKO15_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD6361458.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="KKO15_002729"
     CDS             complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="KKO15_002729"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBD6361459.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="KKO15_002730"
     CDS             complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="KKO15_002730"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBD6361460.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="KKO15_002731"
     CDS             complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="KKO15_002731"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBD6361461.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18356..18475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="KKO15_002732"
     CDS             complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="KKO15_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBD6361462.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18690..19376)
                     /locus_tag="KKO15_002733"
     CDS             complement(18690..19376)
                     /locus_tag="KKO15_002733"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD6361463.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4783 a         5171 c         4786 g         4971 t
ORIGIN      
        1 aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt
       61 ttttgaaccc agtgacgttg aatattactt tgaaccagtg acgatacagg agtcaggaac
      121 cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa taggctttca
      181 actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca
      241 gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa
      301 caatgtctca agcgggcaaa taatgagccg agtcaaaatc gtggtaatgt acaactaatc
      361 tatctccccc accttaactt tgggggagct gccgattaac gcaccgctgg actaaaaagc
      421 gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca
      481 aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta
      541 tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca
      601 ccaaaagctt tcatcaaagc ggcatcatca aactcgccga ggatttctac gtttaatccc
      661 tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa
      721 agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag
      781 aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct
      841 atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt
      901 tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac tgcggcgtta
      961 agtacgctac tgaccaggcg tttggaaagt gcatcagtca cgccaacgtc aaacaataaa
     1021 ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta
     1081 tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt
     1141 ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt
     1201 ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag
     1261 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     1321 gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac
     1381 gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga
     1441 gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga
     1501 tagacatagt aaaaccgata ccgcacagga tccccaccac cataatttgc tgataagtcg
     1561 ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc
     1621 ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc
     1681 catccagcgt gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
     1741 tcagatacgc cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat
     1801 gcttctcttt caaaggaata aagaagccga caattacccc cgccagagtt gcgtgaaccc
     1861 ccgatttcaa caccgcagtc cacaacacca cgccaacaag aatatagacg cccgtgcggc
     1921 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag
     1981 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
     2041 cgataatagc cagagccatc aaaaagatct tcagcactaa cggaacacga cttcccaaca
     2101 gcgccagtac accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc
     2161 gggtaatcgg atcggcatag ttaaaagcca gatagagtaa tgccggcaca atcatcccac
     2221 caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca
     2281 gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta
     2341 tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct
     2401 ccagaaagtc gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga
     2461 tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
     2521 gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcacg
     2581 aatcatcgtt aaaatcagat tatagtgtag gaataattcg ttttttacga gccattgaag
     2641 aatttgcgcc atcagcggtg agatgcgttt ttgacggctg taagcgctca cgagtgaccc
     2701 taagcccaga taatcatagc ttaaggtgag gattcctgag ggattaatta ggaaaagtaa
     2761 taattaattt gttgtcagtc ggcgatgctt gttaggccaa tatttcaaaa ataatccagc
     2821 ttgtcgtcaa taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa
     2881 attacgccac aaaaatgcga ggcgtcttca ggattagaat cgataaaaca aaatgggtta
     2941 aagagagcga tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac
     3001 ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggcct cctatatgct
     3061 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
     3121 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
     3181 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
     3241 tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca
     3301 ggtgctcgca tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt
     3361 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
     3421 gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc
     3481 agctgtttct gcttttcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
     3541 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaatagctta
     3601 ccggtctggg tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg
     3661 atttcgccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg
     3721 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
     3781 ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
     3841 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     3901 catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc
     3961 tgtacagcga agaatccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag
     4021 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     4081 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     4141 ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata
     4201 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accaccgccg
     4261 cccatgcctc cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc
     4321 gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg
     4381 tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttctgatt
     4441 tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc
     4501 ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt
     4561 cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc
     4621 gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg
     4681 ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg
     4741 cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag
     4801 cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg
     4861 cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa
     4921 cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc
     4981 gttcagacca gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt
     5041 cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat
     5101 gccgcgcggt gccgggttga taccatctag gttgaactga cccagagatt tgttatcagc
     5161 cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc
     5221 ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt
     5281 catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac
     5341 gtcttttacg tcgccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc
     5401 gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac
     5461 cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag
     5521 gccagcatcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac
     5581 caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc
     5641 tgcagtgatg tatggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc
     5701 tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc
     5761 ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga agtcttcacc
     5821 acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc
     5881 gtcgatttcg ataatagaaa tatcgaaagt accacccccc aggtcataaa ccgcgatagt
     5941 acggttgcca gtgcctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat
     6001 gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc
     6061 atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc
     6121 ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt
     6181 ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat
     6241 gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat
     6301 cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag
     6361 agtttcacca tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt
     6421 ctccagcacg cgaggaatgg tgccatccat aatcgctaca caagagttgg tagtacccag
     6481 gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg
     6541 ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc
     6601 agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag
     6661 atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga caagacgtaa
     6721 aattgacagc aattttattc atataaagaa tgaatcgcag ccaaggcgtt catcaaaaaa
     6781 ttgtaataaa aagaaaagat tacgtgcctg aatcttctct ttatcagcag taaactagtg
     6841 ggtattcatc cccctacctc ttcccactaa gagaatcctt atgaaatccg tttttacgat
     6901 ttccgccagc ctggcgatta gcctgatgct gtgctgcacg gcgcaggcaa acgaccataa
     6961 actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac gatctggcgc tgaaacttcc
     7021 tgtttgtcgc attgtgaaac gcatacaact ctctgccgac catggcgatt tacagttaag
     7081 tggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc
     7141 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa
     7201 acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac
     7261 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     7321 attacctgaa tgattcagat ctggattttc ttcagcattg tagtgaggaa cagttggcaa
     7381 atttcgcccg attgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcatactga
     7441 tgcgcaacga actgtttaaa tcgatggaag ggcatcccga gcaacatcgc cgcaactggc
     7501 agctgattgc cggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg
     7561 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaagcgattg aagctgaaag
     7621 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     7681 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     7741 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     7801 gtgtgtcgca tttgctttcc agccaactga cccgcatttt acgcacccac gcagcaatga
     7861 gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg aggccctgtg ggcgcagcat
     7921 taaatggggt taaagcggtc agcggcagca cctatcgcgt gacgattcca gccgtactgc
     7981 aaatcgcctg cctgcgccgg atggttagcg ccactcaggt ctgataccgg aaaccgggga
     8041 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     8101 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     8161 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     8221 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt
     8281 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     8341 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     8401 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     8461 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     8521 actctgaaag gcgcgcgcgt cctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     8581 ctgctggcga tcggtaacat tgccggtaat gccgcgatca tccactttgc cggctggatt
     8641 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtgct gaacgagcag
     8701 tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgtcttgaga tccccccgct
     8761 cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgcagcaa
     8821 ccacttctgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     8881 tggcaaaaat accatgcacc acaacgttac cctcagcgtc cttcacacct tccagcgtct
     8941 ctttaataga cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacgc
     9001 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     9061 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     9121 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     9181 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     9241 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgctggaatg cctttatcct
     9301 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     9361 tcaagatttt tccgtttaaa caatctcgtt aaacagaatg atacactgcg aagggagtga
     9421 cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     9481 gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt
     9541 ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag
     9601 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     9661 cttcgctctc cgccagctct ttcagcagtg ccggtgcgat ggtcagacgg tcacagcctg
     9721 ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac
     9781 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     9841 gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg
     9901 gcgagatcag gaacacgccc gcctccgcac aagcacgagc ctgagcgaag gagaacagca
     9961 gggtcaggtt acagttgata ccttcttttt ccagctgttc tgcagcacgg ataccctgcc
    10021 aggtagaagc cagtttgatc agaatacgat cgttgctaat acctgcatcg ttgtagagtt
    10081 tgatcaggcg ttttgctttc gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa
    10141 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
    10201 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
    10261 caacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag
    10321 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
    10381 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
    10441 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
    10501 gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca
    10561 ctgcgttgat atcgcccacc atttttatac aaaacctcat gtatgctacg cagaagttat
    10621 caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca
    10681 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
    10741 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
    10801 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
    10861 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
    10921 gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg accaggtgcc
    10981 gtcttttgga tgtgggttgc tgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
    11041 cttgcacaac tttataaaga acgtgacgtc aatgggcagt ttcgtggcgg accggcatgg
    11101 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc
    11161 atcgcctacg gcataatttt cagcggagtt caggcgaacg ccgttgcccg agccctgagt
    11221 ttttcttttg attttcctcc gctggtgacg ggcattattc tcgctgtctt tgctctgctg
    11281 gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
    11341 gcgataatct gggtactgac cagcctggtg atttgcgtaa taaatatcgg gcaacttccc
    11401 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
    11461 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
    11521 gaggtgggaa tgggatcgac gccaaacgcg gcagcggcag ctgcatcctg gcctccgcat
    11581 ccggcagcac aagggattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
    11641 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
    11701 ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctaggg tgctgaattt
    11761 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
    11821 gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc
    11881 tgcaccttcg caacggtcat cggcggcacc ttgctaagcc ttccgctgat gtggcaactg
    11941 gcagatatca taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
    12001 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
    12061 gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctccgga cgcgtgggat
    12121 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
    12181 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
    12241 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
    12301 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
    12361 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
    12421 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
    12481 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
    12541 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
    12601 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
    12661 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
    12721 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
    12781 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
    12841 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
    12901 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
    12961 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
    13021 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
    13081 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
    13141 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
    13201 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
    13261 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
    13321 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
    13381 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    13441 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
    13501 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
    13561 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
    13621 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
    13681 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
    13741 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
    13801 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
    13861 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
    13921 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
    13981 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
    14041 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
    14101 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
    14161 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
    14221 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
    14281 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
    14341 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
    14401 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
    14461 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
    14521 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
    14581 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
    14641 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
    14701 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
    14761 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
    14821 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
    14881 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
    14941 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
    15001 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
    15061 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
    15121 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
    15181 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
    15241 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
    15301 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
    15361 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
    15421 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
    15481 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
    15541 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
    15601 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
    15661 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
    15721 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
    15781 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
    15841 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
    15901 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
    15961 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
    16021 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
    16081 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
    16141 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
    16201 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
    16261 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
    16321 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
    16381 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
    16441 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
    16501 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
    16561 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc
    16621 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
    16681 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
    16741 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
    16801 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
    16861 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
    16921 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
    16981 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
    17041 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
    17101 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
    17161 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
    17221 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
    17281 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
    17341 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
    17401 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
    17461 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
    17521 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
    17581 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
    17641 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
    17701 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
    17761 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
    17821 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
    17881 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
    17941 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
    18001 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
    18061 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
    18121 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
    18181 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
    18241 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
    18301 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
    18361 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
    18421 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
    18481 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
    18541 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
    18601 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
    18661 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
    18721 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    18781 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
    18841 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    18901 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    18961 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    19021 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    19081 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    19141 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    19201 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    19261 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    19321 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    19381 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    19441 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    19501 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    19561 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    19621 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    19681 acgatgattt catcaaactg ttaacgtgct a
//
LOCUS       DAECOD010000077        19377 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.077, whole genome shotgun sequence.
ACCESSION   DAECOD010000077 DAECOD010000000
VERSION     DAECOD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.077"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..193
                     /locus_tag="KKO15_002734"
     CDS             <1..193
                     /locus_tag="KKO15_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD6361464.1"
                     /translation="ALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRR
                     CDVLFAMMRDRTFYTPQGS"
     gene            219..2164
                     /locus_tag="KKO15_002735"
                     /pseudo
     CDS             219..2164
                     /locus_tag="KKO15_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            2314..2745
                     /gene="ndk"
                     /locus_tag="KKO15_002736"
     CDS             2314..2745
                     /gene="ndk"
                     /locus_tag="KKO15_002736"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBD6361465.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2895..4049
                     /gene="trmG/rlmN"
                     /locus_tag="KKO15_002737"
     CDS             2895..4049
                     /gene="trmG/rlmN"
                     /locus_tag="KKO15_002737"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBD6361466.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4334..5347
                     /gene="rodZ"
                     /locus_tag="KKO15_002738"
     CDS             4334..5347
                     /gene="rodZ"
                     /locus_tag="KKO15_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBD6361467.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5374..6501
                     /gene="ispG"
                     /locus_tag="KKO15_002739"
                     /gene_synonym="gcpE"
     CDS             5374..6501
                     /gene="ispG"
                     /locus_tag="KKO15_002739"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBD6361468.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6604..7878
                     /gene="hisS"
                     /locus_tag="KKO15_002740"
     CDS             6604..7878
                     /gene="hisS"
                     /locus_tag="KKO15_002740"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBD6361469.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7896..8516
                     /gene="yfgM"
                     /locus_tag="KKO15_002741"
     CDS             7896..8516
                     /gene="yfgM"
                     /locus_tag="KKO15_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBD6361470.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8527..9705
                     /gene="bamB"
                     /locus_tag="KKO15_002742"
     CDS             8527..9705
                     /gene="bamB"
                     /locus_tag="KKO15_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBD6361471.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9823..11295
                     /gene="der"
                     /locus_tag="KKO15_002743"
     CDS             9823..11295
                     /gene="der"
                     /locus_tag="KKO15_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBD6361472.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11365..11580
                     /locus_tag="KKO15_002744"
     CDS             11365..11580
                     /locus_tag="KKO15_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBD6361473.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11577..12947)
                     /gene="xseA"
                     /locus_tag="KKO15_002745"
     CDS             complement(11577..12947)
                     /gene="xseA"
                     /locus_tag="KKO15_002745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBD6361474.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13109..14575
                     /gene="guaB"
                     /locus_tag="KKO15_002746"
     CDS             13109..14575
                     /gene="guaB"
                     /locus_tag="KKO15_002746"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBD6361475.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14644..16221
                     /gene="guaA"
                     /locus_tag="KKO15_002747"
     CDS             14644..16221
                     /gene="guaA"
                     /locus_tag="KKO15_002747"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBD6361476.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16316..16855)
                     /locus_tag="KKO15_002748"
     CDS             complement(16316..16855)
                     /locus_tag="KKO15_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBD6361477.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16871..17389)
                     /locus_tag="KKO15_002749"
     CDS             complement(16871..17389)
                     /locus_tag="KKO15_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD6361478.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17700..17891)
                     /locus_tag="KKO15_002750"
     CDS             complement(17700..17891)
                     /locus_tag="KKO15_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBD6361479.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17909..18061
                     /locus_tag="KKO15_002751"
     CDS             17909..18061
                     /locus_tag="KKO15_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361480.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18243..>19377
                     /gene="pdeF"
                     /locus_tag="KKO15_002752"
     CDS             18243..>19377
                     /gene="pdeF"
                     /locus_tag="KKO15_002752"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD6361481.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4566 a         4726 c         5265 g         4820 t
ORIGIN      
        1 agctctcaaa cgggcgttgt tcctgtcggc cttcgccgcg ctcagggatc cgctctccag
       61 ggcttactac acccgcaaaa tgagtcaggg aaaacgacac aatcaggcgc ttatcgccct
      121 ggcgagacga cgctgcgacg ttctgttcgc catgatgcgc gacaggactt tttatacccc
      181 gcaggggtca taacatgctt gacaacttaa taggggcacg ttaccttgaa gcgctgatca
      241 attatgaaga tcgctggttc tggaagcatc cgggggttaa tccattctcg gtggcgcgcg
      301 cagcatggca agatctcact tcgggacggg ttatttccgg tggcagcacg ctcactatgc
      361 aggttgctcg tctgcttgat cctcacccca aaacatttgg tggcaaaatt ctccagctct
      421 ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga aattctgatc ttgtatctta
      481 accgcgctcc gtttggcggt acgttgcagg ggatcggtgc ggcaagttgg gcttatctcg
      541 gaaaatcgcc tgcgaattta agctattccg aggcggcaat gctggcggtg ttaccgcaag
      601 tgcccagccg actgcgcccg gatcgttggc cggagcgtgc cgaagccgcg cgtaataaag
      661 tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga gcaggtaaaa gagtcaaggg
      721 aagaacccat ctggctggca ccccgacaaa tgccgcaact ggcaccgctg ttttcgcgca
      781 tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac gctggatgcc ggtcttcaac
      841 gacgtctgga agaactggcg caaaactgga aagggcggtt gccaccgcgc agctcactgg
      901 cgatgatcgt ggttaatcat accgatatgc gtgttcgcgg ctgggtggga tcggttgatc
      961 tcaacgatga ttcacgcttt ggtcatgttg atatggtcaa tgcgatccga tcgccaggat
     1021 cggtgctcaa accgtttgtt tatggtctgg cgctggatga aggcttgatc cacccggcat
     1081 cactgctgca agacgtcccc cggcgcaccg gtgattatcg accaggtaac tttgatagcg
     1141 gttttcatgg cccgatcaac atgagcgagg cgctggtgca aaactggaag atatggcggc
     1201 agcgtatacc gcgtttgctc gccacggcaa ggcaggcaaa ttgcgcttac agcctgacga
     1261 tccgctgctt gaacggcctt taatgtcgtc gggggcggcg tggatcattc gccggattat
     1321 ggctgatgaa gcgcaaccct tgccggatag tgccttgccg cgcgtcgccc cactggcatg
     1381 gaaaacgggc accagctatg gctatcgtga cgcctgggcg attggggtta acgctcgcta
     1441 tgtcattggg atctggactg gcagacctga cggcacgccc gttgttggtc agtttggctt
     1501 tgccagtgcc gtaccattgt tgaatcaggt caataatatc ttactgtcgc gcagtgcgaa
     1561 tctgccggaa gacccgcgcc cgaactcagt cactcgtggc gttatctgct ggccgggcgg
     1621 acagtctttg ccggaaggtg acggtaactg tcgccgccgc ctggcaacct ggctgctgga
     1681 tggcagccag ccgccaacgc tgttactgtc agagcaggaa gggattaatg gcattcgctt
     1741 ccccatctgg ctggatgaca aagggcagcg cgtcgccgct gactgcccgc aggcgaagca
     1801 agaaatggta aatgtctggc cgctaccgct ggaaccctgg ctgcccgcat cagaacgtcg
     1861 cgctgtgcgc ttaccaccgg cctcgacaat ttgcccgcca tacggtcacg atgcccagct
     1921 ccctctgcag ctgacaggcg ttcgcgatgg cgcgattatt aaacgtttac cgggcgcagc
     1981 ggaagcgact ttgccgttgc aatcaagtgg aggggcaggt gaacgctggt ggtttctgaa
     2041 tggcgaaccg ttaactgaac gcgggcgcaa cgtgaccctg catttgacgg ataaaggtga
     2101 ttatcaattg ctggtgatgg atgatgtggg gcaaatcgcg acagtgaaat ttgtcatgca
     2161 atagtctgtt ttgtccctga ttgttgctaa aactcatttt attttaaaaa aaatgttacc
     2221 ttcatcaata gtcaacggcc ctgttgctca ttataatccg cgccatctcg tacgctggta
     2281 cagacaacaa cagaacaatt tacagaggta aaaatggcta ttgaacgtac tttttccatc
     2341 atcaaaccga acgcggtagc aaaaaacgtc attggtaata tctttgcgcg ctttgaagct
     2401 gcagggttca aaattgtcgg caccaaaatg ctgcatctga ccgttgaaca ggcacgtggc
     2461 ttttatgctg aacacgatgg aaaaccgttc tttgatggtc tggttgaatt catgacctct
     2521 ggcccgatcg tggtttccgt gctggaaggt gaaaacgccg ttcagcgtca ccgcgatctg
     2581 ctgggcgcga ccaatccggc aaacgcactg gctggtactc tgcgcgctga ttacgctgac
     2641 agcctgaccg aaaacggtac ccacggttct gattccgtcg aatctgccgc tcgcgaaatc
     2701 gcttatttct tcggcgaagg cgaagtgtgc ccgcgcacct gttaataatt tcgtaaatgc
     2761 cgcgtgcaaa cgtggcatcc gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc
     2821 agccgctcac cggggcgttt cttttttcaa ccctccaggg gccataacgt gtaataacga
     2881 ggccggaata gcatatgtct gaacaattag tcacacctga aaacgtcacc acgaaagatg
     2941 gaaaaatcaa cctgctggat ctcaaccgtc agcagatgcg ggagtttttt aaagatttag
     3001 gtgaaaaaac cttccgcgcc gatcaggtga tgaagtggat gtatcactat tgctgcgaca
     3061 actttgatga gatgaccgac atcaacaaag tgttgcgcgg caaactgaaa gaggtggcgg
     3121 aaatccgcgc accggaagtg gttgaagaac agcgttcatc cgacggcacc attaaatggg
     3181 cgatcgccgt tggcgatcag cgcgtcgaaa cggtgtatat cccggaagac gaccgtgcca
     3241 cgctctgcgt ctcttcgcag gtggggtgtg cgctggagtg taaattctgt tccaccgccc
     3301 agcagggctt taaccgcaac ctgcgggtat cggaaattat tggccaggtg tggcgtgcgg
     3361 cgaaaatcgt cggcgcagca aaagtcaccg gtcagcgccc aatcactaac gtcgtgatga
     3421 tgggcatggg cgagccgctg ctcaacctga acaacgtcgt tccggcgatg gaaatcatgc
     3481 ttgatgattt cggttttggc ctgtctaaac gtcgcgtgac gctttccact tccggtgtag
     3541 ttccggcgct ggataaactg ggcgatatga tcgacgttgc actggcaatt tccctgcacg
     3601 cgccgaacga cgaaattcgt gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa
     3661 cgttccttgc tgcggtgcgc cgttatctgg agaaatccaa cgccaatcag ggccgagtca
     3721 ctattgaata cgtgatgctt gaccacgtta acgacggcac tgaacacgcg caccaactgg
     3781 cggaactgct gaaagatacg ccgtgtaaga tcaacctgat cccgtggaac ccgttcccgg
     3841 acgcgccgta tggacgcagt tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga
     3901 gctacggttt caccactatt gtgcgtaaaa cgcgtggtga tgatatcgat gctgcctgtg
     3961 gtcagttggc gggcgatgtt atcgaccgta cgaaacgtac tctgcgtaaa cggatgcagg
     4021 gtgaagccat cgatattaaa gcggtctgat agccattacg ccacggttac tttatgtacc
     4081 gtggcgtaat cttttatgaa tgaagtatta agttgcaatt gtctggtcaa tattaacggt
     4141 gcgtttttgc tgactttaag gcagtatgta acgatgcaac agtaagttag cctcagtgaa
     4201 tgtgggcttt gtcacgagca cacagacggt cttatactgt atgataacgg ttaacttaac
     4261 ggatgtttcg cggtgtgggt gagcatgatg gttcaccggc atctcaattc tcatttaaac
     4321 gtacctgcag cgaatgaata ctgaagccac gcacgaccaa aatgaagcac ttactaccgg
     4381 cgctcgcctg cgtaatgctc gcgaacaact aggacttagt cagcaggccg ttgccgagcg
     4441 actttgcctg aaggtttcca cggtacgcga cattgaagaa gataaggcac ccgccgatct
     4501 tgcttcaaca ttcctgcgcg gatatatccg ctcttatgcg cgtctggtac atattccaga
     4561 agaagaactg ctgccagggc tggaaaagca ggctccactt cgggctgcaa aagttgcgcc
     4621 aatgcagagt ttttccctcg gtaaacgccg caaaaaacgc gacggctggc taatgacctt
     4681 cacctggctg gtgttgtttg tggttatcgg cctgagcggt gcctggtggt ggcaagacca
     4741 caaagctcag caggaagaga tcaccactat ggccgatcaa tcttcggcgg aactgagcag
     4801 taatagcgag caggggcaga gtgttccgtt aaatacgtcg acaactacag acccggctac
     4861 aacgtccacg ccgccagcgt ctgtggatac taccgcaacc aacacgcaaa cacctgccgt
     4921 aactgcgcca gcaccagctg ttgatccgca acagaatgcg gttgtttcgc cttcgcaggc
     4981 aaatgttgat accgccgcga ccccggtacc aacggcagca acaacgccag atggtgctgc
     5041 gccgttgcca accgatcagg ctggcgtgac cacgccggcg gctgatccga atgcgctggt
     5101 gatgaacttt actgccgatt gctggctgga ggtcactgat gctaccggta aaaaattgtt
     5161 tagcgggatg cagcgtaaag acggtaattt gaatttaacc ggccaggcac cgtataaact
     5221 gaaaattggt gcgccagccg cagtacagat ccagtatcaa gggaaacctg tcgatctgag
     5281 tcgttttatc agaactaacc aggttgcgcg tctgaccctc aatgccgaac aatcaccggc
     5341 gcagtaacag acgggtaacg cgggagattt ttcatgcata accaggctcc aattcaacgt
     5401 agaaaatcaa cacgtattta cgttgggaat gtgccgattg gcgatggtgc tcccatcgcc
     5461 gtacagtcca tgaccaatac gcgtacgaca gatgtcgaag caacggtcaa tcaaatcaag
     5521 gcgctggaac gcgttggcgc tgatatcgtc cgtgtctccg taccgacgat ggacgcggca
     5581 gaagcgttca aactcatcaa acagcgggtt aacgtgccgc tggtggctga catccacttc
     5641 gactatcgca ttgcgctgaa agtagcggaa tacggcgtcg attgtctgcg tattaaccct
     5701 ggtaatatcg gtaatgaaga gcgtattcgc atggtggttg actgtgcgcg cgataaaaac
     5761 attccgatcc gtattggcgt taacgccgga tcgctggaaa aagatctgca agaaaagtat
     5821 ggcgaaccga cgccgcaggc gttgctggaa tccgccatgc gtcatgttga tcatctcgat
     5881 cgcctgaact tcgatcagtt caaagtcagc gtgaaagcgt ctgacgtctt cctcgctgtt
     5941 gagtcttatc gtttgctggc aaaacagatc gatcagccgc tgcatctggg gatcaccgaa
     6001 gccggtggtg cacgcagcgg ggcagtaaaa tccgccattg gtttaggtct gctgctgtcg
     6061 gaaggcatcg gcgacacgct gcgtgtatcg ctggcggccg atccggtcga agagatcaaa
     6121 gtcggtttcg atattttgaa atcgctgcgt atccgttcgc gagggattaa cttcatcgcc
     6181 tgcccgacct gttcgcgtca ggaatttgat gttatcggta cggttaacgc gctggagcaa
     6241 cgcctggaag atatcatcac tccgatggac gtttcgatta tcggctgcgt ggtgaatggc
     6301 ccaggtgagg cgctggtttc tacactcggc gtcaccggcg gcaacaagaa aagcggcctc
     6361 tatgaagatg gcgtgcgcaa agaccgtctg gacaacaacg atatgatcga ccagctggaa
     6421 gcacgcattc gtgcgaaagc cagtcagctg gacgaagcgc gtcgaattga cgttcagcag
     6481 gttggaaaaa taataacgtg atgggaagcg cctcgcttcc cgtgtatgat tgaacccgca
     6541 tggctcccga aacattgagg gaagcgttga gggttcattt ttatattcag aaagagaata
     6601 aacgtggcaa aaaacattca agccattcgc ggcatgaacg attacctgcc tggcgaaacg
     6661 gccatctggc agcgcattga aggcacactg aaaaacgtgc tcggcagcta cggttacagt
     6721 gaaatccgct tgccgattgt agagcagacc ccgctattca aacgtgcgat tggtgaagtc
     6781 accgacgtgg ttgaaaaaga gatgtacacc tttgaggatc gcaatggcga cagcctgact
     6841 ctgcgccctg aagggacggc gggctgtgta cgcgccggca tcgagcatgg tcttctgtac
     6901 aatcaggaac agcgtctgtg gtatatcggg ccgatgttcc gtcacgagcg tccgcagaaa
     6961 gggcgttatc gtcagttcca tcagttgggc tgcgaagttt tcggtctgca aggtccggat
     7021 atcgacgctg aactgattat gctcactgcc cgctggtggc gtgcgctggg tatcttcgaa
     7081 cacgtaactc ttgagctgaa ttctatcggt tcgctggaag cacgcgccaa ttaccgcgat
     7141 gcgctggtgg cattccttga gcagcataaa gaaaagctgg acgaagactg caaacgccgc
     7201 atgtacacta acccgctgcg cgtgctggat tcaaaaaatc cggaagtgca ggcgcttctc
     7261 aacgacgctc cggcattagg tgattatctg gacgaggaat ctcgtgagca ttttgccggt
     7321 ctgtgcaaac tgctggagag cgcggggatc gcttacaccg taaaccagcg tctggtgcgt
     7381 ggtctggatt actataaccg taccgttttc gagtgggtga ctaacagtct cggctcccag
     7441 ggcaccgtgt gtgcaggcgg tcgttatgac ggtcttgtgg aacaactggg cggtcgtgca
     7501 acaccggctg tcggttttgc gatgggcctc gaacgtcttg tattgttagt acaggccgtt
     7561 aatccggaat ttaaagccga tcctgttgtc gatatatacc tggtggcttc aggtgctgat
     7621 acacaatctg cggctatggc attagctgag cgtctgcgtg atgaattact gggcgtgaaa
     7681 ttgatgacca accacggcgg cggcaacttt aagaaacagt ttgcccgtgc tgataaatgg
     7741 ggtgcccgcg ttgctgtggt gctgggtgag tctgaagtgg ctaacggcac agcagtagtg
     7801 aaggatttgc gctctggtga gcaaacggca gttgcgcagg atagcgtagc cgcgcatttg
     7861 cgcacgttac tgggttaagg aaggagaagg acagcgtgga aatttacgag aacgaaaacg
     7921 accaggtaga agcggttaaa cgcttttttg ctgaaaatgg caaagcactg gctgttgggg
     7981 ggattttggg cgttggcgca ctgattggct ggcgctactg gaacagccat caggttgatt
     8041 ctgcacgctc cgcttctctt gcctatcaaa atgcggttac cgcagtgagc gaaggcaaac
     8101 cggatagcat cccggcggcg gaaaaatttg ctgctgaaaa taaaaatact tatggtgcgc
     8161 tggcttcttt ggaacttgcg cagcaatttg ttgacaaaaa tgaactagag aaagctgccg
     8221 cccagttaca acaggggctg gcagacacga gcgatgaaaa tctcaaagcc gtgataaatc
     8281 tgcgtcttgc tcgcgttcag gtacagctca agcaggctga tgccgcgctg aaaacccttg
     8341 ataccatcaa aggtgaaggg tgggctgcca ttgttgccga cttgcgtggt gaagcattgc
     8401 tgagcaaagg tgataagcaa ggtgcgcgta gtgcatggga agcaggcgtg aaaagcgatg
     8461 ttactccggc actgagcgaa atgatgcaga tgaaaattaa taatttgtcc atctgagagg
     8521 gacccgatgc aattgcgtaa attactgctg ccaggactgc tttccgttac ccttttaagc
     8581 ggctgttcgc tgtttaacag cgaagaagat gtggtaaaga tgtccccatt gccaaccgtt
     8641 gaaaaccagt ttacgccgac cacggcgtgg agcacttccg ttggtagcgg cattggcaac
     8701 ttctattcca atcttcatcc ggcactggcg gacaacgttg tctatgcagc ggaccgcgct
     8761 ggtttggtaa aagcgctgaa tgcggatgat ggcaaagaaa tctggtctgt cagcctggcc
     8821 gagaaagatg gctggttctc taaagagcct gcattacttt ctggcggtgt gaccgtgtct
     8881 ggtgggcatg tctacattgg cagcgaaaag gcgcaggttt acgcgctgaa taccagcgat
     8941 ggtactgtgg catggcaaac taaagtcgcg ggtgaagcac tttcgcgccc ggtggtcagc
     9001 gacggtctgg tgttaatcca caccagtaac ggtcagttac aagcgctgaa cgaagctgac
     9061 ggcgctgtca aatggacagt taacctcgat atgccttcgc tctctttgcg tggcgagtct
     9121 gcgccggcaa cggcttttgg tgcggccgtc gtgggtggcg ataatggtcg cgtcagcgca
     9181 gtgctgatgg aacagggcca gatgatttgg cagcagcgta tttcccaggc gaccggttct
     9241 accgaaattg accgtctgag cgatgttgac acgactcccg tcgttgttaa cggcgttgtt
     9301 ttcgcgctgg cctataatgg taacctgacg gcgcttgatc tgcgcagtgg tcagattatg
     9361 tggaaacgcg aactgggttc ggtgaatgat ttcatcgtcg atggcaatcg catctatctg
     9421 gtcgatcaaa atgaccgggt gatggcgttg accattgatg gcggcgttac gctgtgggca
     9481 caaagcgatc tgctgcatcg cctgctgact tctccggtgc tgtataatgg caacctggtg
     9541 gtcggtgaca gtgaaggtta tctgcactgg attaacgtcg aagatggtcg tttcgttgcc
     9601 cagcaaaaag ttgatagttc cggtttccag actgaaccgg ttgccgctga cggcaaactg
     9661 ctgatccagg caaaagacgg aaccgtgtac tctattacac gttaatcgtc tctgtcgttc
     9721 actttgaaaa cggctcctgg acaggggccg ttttcctgtt tttaacaacg acgcgaatat
     9781 agcgtgcgtt gtctgatgat ttataaaaat gaggctttaa acatggtacc tgtggtcgcg
     9841 cttgtcgggc gccctaacgt aggaaaatcc acgttattta accgtctaac tcgcacccga
     9901 gatgcgctgg ttgcggattt cccgggtctg actcgtgacc gtaagtacgg tcgtgcggaa
     9961 attgaaggcc gtgagtttat ctgtattgat accggcggga ttgatggcac agaagacggt
    10021 gtagaaaccc gcatggcgga acagtcgctg ctggcgattg aagaagcgga cgtcgtactg
    10081 tttatggtgg atgcgcgcgc gggcctgatg ccggcagatg aagcgattgc caaacatctg
    10141 cgctcccgtg aaaaaccgac cttcctggtg gtaaacaaaa ctgacggtct ggatcccgat
    10201 caggcagtgg ttgatttcta ctcgcttggt ttaggtgaaa tctacccgat cgccgcgtct
    10261 cacggtcgtg gcgtattaag tctgctggag catgtgctgc tgccgtggat ggaagatctc
    10321 gcaccgcaag aggaagtcga cgaagacgct gaatactggg cgcaatttga agcggaagag
    10381 aacggcgaag aagaagagga agacgacttc gacccgcaaa gtctgccgat caaactggcg
    10441 attgtgggtc gtccgaacgt aggtaagtct acactcacta accgtattct tggagaagag
    10501 cgcgttgttg tttacgacat gcctggcacg acgcgtgaca gcatctacat cccaatggaa
    10561 cgcgatggac gtgagtatgt gctcattgac accgctggcg tacgtaaacg cggcaaaatc
    10621 accgatgctg tagagaaatt ctccgtaatc aaaacgttgc aggccattga agacgccaac
    10681 gtggtgatgt tagtgattga tgcgcgcgaa ggtatttccg atcaggatct ctcgctgctg
    10741 ggctttattc tcaatagtgg gcgctcactt gtcattgtgg tgaataagtg ggatggcctg
    10801 agccaggaag tgaaagagca ggtgaaagaa acgctggact tccgtctggg ctttatcgat
    10861 tttgctcgtg tgcactttat ctctgccttg cacggcagtg gtgttggtaa cttgtttgaa
    10921 tcagtacgtg aagcgtatga cagctccacc cgtcgtgtgg ggacctctat gctgacgcgc
    10981 atcatgacga tggctgttga agatcaccaa ccgccgctgg tacgcggtcg tcgtgtgaag
    11041 ctgaaatatg cccacgctgg tggttataac ccgccgattg tggtgattca cggtaatcag
    11101 gtgaaagacc tgcctgattc ctacaagcgc tacttgatga actacttccg caaatcgctg
    11161 gacgtaatgg gatcgccgat tcgtattcag ttcaaagaag gggaaaaccc gtatgcgaac
    11221 aagcgtaaca ccctgacgcc aacccagatg cgtaaacgta agcgtctgat gaagcacatc
    11281 aagaaaaata aataattatc cgtaagtatt ttatctgcca ttccctctat gaatgtagag
    11341 ggaatggcta ccggaggaat tcatatggaa ctgcattgtc caaaatgtca gcatgtgctt
    11401 gatcaggata acggccatgc gcgttgccca tcctgcgggg aatttattga aatgaaagct
    11461 ctttgtccag actgccatca accgttgcag gtgttaaaag cctgcggtgc agtagattac
    11521 ttctgtcagc acgggcatgg cttgatatcg aaaaaacgcg ttgaattcgt gctggcttaa
    11581 tgaacctttt tacgcgattt ttttaccggc tggatgtttt ttacttcact ttctatccag
    11641 ccgtcttcca gacgtgtggt tagcatttca cccgttttaa cttgcttcac ttttttcagt
    11701 acattgccgt cagtagcagt agtaacgctg tatccacgcg ccagcgttga cagtgggctt
    11761 acggcttcga ggtgcgttac tgcattaccg aaacgttcac gcgtggcgct aagctgtgcg
    11821 cgcaggattt ctgctaaacg atattccagt tgctgaatgc gcgtttgcgt gcgatgaatc
    11881 ttcggctgtg gattttgctg attcagccgc tgtgttaatc gctgctgctg ttgcccggca
    11941 cgcttaagct gattttccag cgcaaagctc atccgttttt gcaggcgttc aagcatggtt
    12001 tgctggcgtg ccagccggag ctgcggatgc tgctgctgca agcgatgatg aatctgcgta
    12061 aagcgacgtg tacggttggc gagataataa tccatcgcca tctccagccg ttgacgggcc
    12121 gattgcacct ggcgcagtaa ctcttgctga ttacggctca ctacttcagc ggcggcagac
    12181 ggcgttggcg cacgcagatc ggcaacaaaa tcggcaatgg tcacatccgt ctcatgcccg
    12241 acggcgctga cgaccggaat gcggctggca aaaatcgccc gcgctacgcg ttcgtcgtta
    12301 aaactccata aatcttccag cgaaccgccg ccgcgcccaa cgatcaacac gtcgcactca
    12361 ttgcgctgat tcgccagttc aatggcgcga acaatttgcc ccggcgcgtc atcgccctga
    12421 acggaggtgg ggtagatgat caccggtaga gaaggatcgc gacgttttaa cacatgcaaa
    12481 atatcatgta gcgcagcacc ggtttttgag gtgatcacac caacgcaatg cgcaggggag
    12541 ggaagtggtt ttttgtattg ctgatcgaac aaaccttcag cctgcaactt cgctttgagc
    12601 tgttcgtact tctgttgcag cagcccttca ccggccggct gcatactctc aacgattatc
    12661 tggtagtcgc cgcgcggctc gtagagcgta atattggcgc gaactaaaac ttgttgccca
    12721 tgctgcggac ggaaggtgac ccggcggttg ctgttgcgga acatcgcgca gcgtacctgg
    12781 gcggtgtcgt ctttgagtgt aaagtaccag tgaccggaag ccggttgtgt gaaattagag
    12841 atttcgccgc tgatccaaac ctgtcccatc tcatgctcaa gcagcagacg aaccgtttga
    12901 ttcaggcgac taacggtaaa aattgcaggg gattgagaag gtaacatgtg agcgagatca
    12961 aattctaaat cagcaggtta ttcagtcgat agtaacccgc ccttcgggga tagcaagcat
    13021 tttttgcaaa aaggggtaga tgcaatcggt tacgctctgt ataatgccgc ggcaatattt
    13081 attaaccact ctggtcgaga tattgcccat gctacgtatc gctaaagaag ctctgacgtt
    13141 tgacgacgtt ctcctcgttc ctgctcattc taccgttctg ccgaatactg ctgacctcag
    13201 cacccagctg acgaaaacta ttcgtctgaa tatccctatg ctttccgcag caatggatac
    13261 cgtaacggaa gcgcgcctgg ctattgctct ggctcaggaa ggcggtatcg gctttatcca
    13321 caaaaacatg tccattgaac gccaggcaga agaagttcgc cgtgtgaaaa aacacgaatc
    13381 tggtgtggtg actgatccgc agactgttct gccaaccacg acgctgcgcg aagtgaaaga
    13441 actgaccgag cgtaacggtt ttgcgggcta tccggtcgtt accgaagaaa acgaactggt
    13501 gggtattatc accggtcgtg acgtgcgttt tgttaccgac ctgaaccagc cggttagcgt
    13561 ttacatgacg ccgaaagagc gtctggtcac cgtgcgtgaa ggtgaagccc gtgaagtggt
    13621 gctggcaaaa atgcacgaaa aacgcgttga aaaagcgctg gtggttgatg acgaattcca
    13681 cctgatcggc atgatcaccg tgaaagactt ccagaaagcg gaacgtaaac cgaacgcctg
    13741 taaagacgag caaggccgtc tgcgtgttgg tgctgcagtt ggcgcaggtg cgggtaacga
    13801 agagcgtgtt gacgcgctgg ttgccgctgg cgttgacgtt ctgctgatcg actcctccca
    13861 cggtcactca gaaggcgttt tgcagcgtat ccgtgaaacc cgtgctaaat atccggatct
    13921 gcaaatcatc ggcggcaacg tggcaacagc tgcaggtgca cgcgctttgg cagaagctgg
    13981 ttgcagcgcg gttaaagtcg gtatcggccc tggttctatc tgtacgactc gtatcgttac
    14041 tggcgtaggt gttccgcaga tcactgccgt tgctgacgca gttgaagccc tggaaggcac
    14101 cggtattccg gttatcgcag atggtggtat tcgcttctcc ggcgacatcg ccaaagctat
    14161 cgccgctggc gcaagcgcgg tgatggtagg ttccatgctg gcgggtactg aagaatctcc
    14221 gggtgaaatc gaactctacc agggccgttc ttataaatct taccgtggta tgggttccct
    14281 gggcgcgatg tccaaaggtt cctctgaccg ttatttccag agcgataacg ctgccgacaa
    14341 actggtgccg gaaggtatcg aaggtcgcgt agcttataaa ggtcgcctga aagagatcat
    14401 tcaccagcag atgggcggcc tgcgctcctg tatgggtctg accggctgtg gtactatcga
    14461 cgaactgcgt actaaagcgg agtttgtacg tatcagcggt gcgggcattc aggaaagcca
    14521 cgttcacgac gtgaccatta ctaaagagtc cccgaactac cgtctgggct cctgattctc
    14581 ttcgcccgat ttcatgtcgg gcgatttata ttatctgttt cacttgcctc ggaataagcg
    14641 tcaatgacgg aaaacattca taagcatcgc atcctcattc tggacttcgg ttctcagtac
    14701 actcaactgg ttgcgcgccg cgtgcgtgag ctgggtgttt actgcgaact gtgggcgtgg
    14761 gatgtgacag aagcacaaat tcgtgacttc aatccaagcg gaattattct ttccggcggc
    14821 ccggaaagta ctactgaaga aaacagtccg cgtgcgccgc agtatgtctt tgaagcaggc
    14881 gtaccgatat tcggcgtttg ctatggcatg cagaccatgg caatgcagtt gggcggtcac
    14941 gttgaagcct ctaacgaacg tgaatttggc tacgcgcagg tagaagtcgt aaacgacagc
    15001 gcactggttc gcggtatcga agatgcgctg accgcagacg gtaaaccgct gctcgatgtc
    15061 tggatgagcc acggcgataa agttaccgct atcccgtccg acttcgtcac cgtagccagc
    15121 accgaaagct gcccgtttgc cattatggct aacgaagaaa aacgcttcta tggcgtacag
    15181 ttccacccgg aagtgactca cacccgccag ggtatgcgca tgctggagcg ttttgtgcgt
    15241 gatatctgcc agtgtgaagc cctgtggacg ccagcgaaaa ttatcgacga tgctgtagct
    15301 cgcatccgcg agcaggtagg cgacgataaa gtcatcctcg gcctctctgg tggtgtggat
    15361 tcctccgtaa ccgcaatgct gctgcaccgt gctatcggta aaaacctgac ttgcgtattc
    15421 gtcgacaacg gcctgctgcg tctcaacgaa gcagagcagg ttctggatat gtttggcgat
    15481 cactttggtc ttaacattgt tcacgtaccg gcagaagatc gcttcctgtc agcgctggct
    15541 ggcgaaaacg atccggaagc aaaacgtaaa atcatcgggc gcgttttcgt tgaagtattc
    15601 gatgaagaag cgctgaaact ggaagacgtg aagtggctgg cgcagggcac catctaccct
    15661 gacgttatcg aatctgcggc ttctgcaacc ggtaaagcac acgtcatcaa atctcaccac
    15721 aacgtgggcg gcctgccgaa agagatgaag atgggcctgg ttgaaccgct gaaagagctg
    15781 ttcaaagacg aagtgcgtaa gattggtctg gagctgggcc tgccgtacga catgctgtac
    15841 cgtcatccgt tcccgggacc aggccttggc gttcgtgttc tgggtgaagt gaagaaagag
    15901 tactgtgacc tgctgcgccg tgctgacgcc atcttcattg aagaactgcg taaagcggac
    15961 ctgtacgaca aagtcagcca ggcgttcacc gtcttcctgc cagtacgttc cgttggcgta
    16021 atgggcgatg gtcgtaagta tgactgggtt gtctctctgc gtgctgtcga aaccatcgac
    16081 tttatgaccg cacactgggc gcatctgccg tacgatttcc tcggtcgcgt ttccaaccgc
    16141 attatcaatg aagtgaacgg tatttcccgc gtggtgtatg acatcagcgg caagccgcca
    16201 gctaccattg agtgggaatg attttgatcc ctgcactatg aatgaacaaa accctccgtt
    16261 actacagagg gttttttatc ttcaagaatc ataggcttga agttactaac atcgattaat
    16321 taaaccagct gtccgatttg ttctcttctg ctttgcccac gcttttcatc agatcgcgac
    16381 cgccttcagt catatttctg tttgcgtcag cttcagattg caccacatcg gtttgcgcag
    16441 ctttgtgctt cagttcctga tcgataaatt cgttttcgcg cttaacgcgg gcttcttctt
    16501 tcgccagcgc cagtttttgt ttctgaatct ctaagctgcg tagctcatct tcataacttt
    16561 gatcgcgttt tttgtccgca gtggcttcgg cgtccagttt atcctgacga gctttcttat
    16621 ttgccgctgc cgttgccgct cttttgttcg cggcggcctg ggcatttgcg cgacgttgct
    16681 tctcttgctg gatttccctg ttgcgctccg cgacccattc gtcatgctgc ctttgctctt
    16741 catttttacc ttgctgttcc gcttctgcga cagcagagag ttgatcctgc aatgatgagg
    16801 ctatagccgg atagcttaag gaggctaaga tggcgcaaag aaaaactttc ttcatgactc
    16861 ctcctgatta ttagctcttt tcaggacatt tggtatttgg ctgaatacgc gtttcgttat
    16921 acgttgtggt aataacaacg gctaaacctg tcgtaaactg gcactcttta ccctcctggg
    16981 tggaggtata cactttggtg ccttccttat atgttaaaga aacaccttcc actaaggttt
    17041 tatcattcac catagaaccc gctgccgcgc ctacagctcc gccgccaact gcccctgccg
    17101 tcgttccgga attgctgcca gaaccgacgt tgtggccgat aacaccgcca gcgactgcgc
    17161 caataagcgc gccgaaggct tgtgcgttcc gtttattttg ggagttgtct acggcaactt
    17221 ttgcgggaag aatggaaata atattaacgg ttttagtttc ttgtttggta ttcagttgat
    17281 cggtttgata aacatcggcg gcatgatcgt cagcatttga ctggcatcct gccagagtga
    17341 atgacgctaa cattgccaca ggcagaagac attttttaaa tttcatcact attccttgtt
    17401 atacacatta cgaaaaataa gacatttaag atcatcaaaa gaggaaagta aatgtattgc
    17461 gtataaaggg atagattaaa tttattaaaa ttcacgcagg gaataattta taaaaatata
    17521 tcattacaac atatatatta attttatatt tatttcgcag ccaaacagga taaacatcag
    17581 tcaggacaaa gacgtaaaat ttaagatgaa agaatatctg ccgctttgat aatcccaaag
    17641 agcaaaagca tccgacagcg aagtgccgtt atgttctgtg aaagtggaga aggtaaccgc
    17701 taacgttgta ccggtgattc gacggagagt gtggattgct gagcttcttt tttgctctga
    17761 tggtgctgcc aggcaccgac ggacgagtag ataaaacggc caaagaagaa gataaagctg
    17821 atgagcagta cgatacgggt catgcgactg ttaaatcggt gtcgttttcg catactagtt
    17881 gcctgactca caaaaggttc cttgaagtat gtcccacgct gtggacggta cttacattaa
    17941 ggcacaacag gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag tggatacata
    18001 ttttaatgtt atggaagtta atttaattat ttacaatgat gatgtaataa tgatgaatta
    18061 acatgataaa cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga
    18121 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
    18181 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
    18241 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
    18301 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
    18361 ccggtgaggt ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt
    18421 tcagctgggc ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc
    18481 tgggttttta cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt
    18541 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
    18601 taatttccca gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt
    18661 tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc
    18721 cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg
    18781 tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt
    18841 attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc
    18901 tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
    18961 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcaatgc
    19021 gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca
    19081 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
    19141 ccagttatct ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc
    19201 gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa
    19261 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
    19321 gcatccctgg catggaaatg ctggttaaga actatggcat catgctgcgg attcaat
//
LOCUS       DAECOD010000078        19375 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.078, whole genome shotgun sequence.
ACCESSION   DAECOD010000078 DAECOD010000000
VERSION     DAECOD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.078"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KKO15_002753"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KKO15_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBD6361482.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KKO15_002754"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KKO15_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBD6361483.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KKO15_002755"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KKO15_002755"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBD6361484.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KKO15_002756"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KKO15_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBD6361485.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KKO15_002757"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KKO15_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBD6361486.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KKO15_002758"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KKO15_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBD6361487.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KKO15_002759"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KKO15_002759"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBD6361488.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KKO15_002760"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KKO15_002760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBD6361489.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KKO15_002761"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KKO15_002761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBD6361490.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KKO15_002762"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KKO15_002762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBD6361491.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KKO15_002763"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KKO15_002763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBD6361492.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KKO15_002764"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KKO15_002764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBD6361493.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KKO15_002765"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KKO15_002765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBD6361494.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KKO15_002766"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KKO15_002766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBD6361495.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KKO15_002767"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KKO15_002767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBD6361496.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KKO15_002768"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KKO15_002768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBD6361497.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KKO15_002769"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KKO15_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBD6361498.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KKO15_002770"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KKO15_002770"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBD6361499.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5123 g         4626 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cataagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgta tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAECOD010000079        18048 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.079, whole genome shotgun sequence.
ACCESSION   DAECOD010000079 DAECOD010000000
VERSION     DAECOD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.079"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            32..1081
                     /locus_tag="KKO15_002771"
                     /pseudo
     CDS             32..1081
                     /locus_tag="KKO15_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1227..2264)
                     /gene="iap"
                     /locus_tag="KKO15_002772"
     CDS             complement(1227..2264)
                     /gene="iap"
                     /locus_tag="KKO15_002772"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBD6361500.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2516..3424
                     /gene="cysD"
                     /locus_tag="KKO15_002773"
     CDS             2516..3424
                     /gene="cysD"
                     /locus_tag="KKO15_002773"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBD6361501.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3426..4853
                     /gene="cysN"
                     /locus_tag="KKO15_002774"
     CDS             3426..4853
                     /gene="cysN"
                     /locus_tag="KKO15_002774"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBD6361502.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4853..5458
                     /gene="cysC"
                     /locus_tag="KKO15_002775"
     CDS             4853..5458
                     /gene="cysC"
                     /locus_tag="KKO15_002775"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBD6361503.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5508..5831
                     /locus_tag="KKO15_002776"
     CDS             5508..5831
                     /locus_tag="KKO15_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBD6361504.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6025..6336
                     /gene="ftsB"
                     /locus_tag="KKO15_002777"
     CDS             6025..6336
                     /gene="ftsB"
                     /locus_tag="KKO15_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBD6361505.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6355..7065
                     /gene="ispD"
                     /locus_tag="KKO15_002778"
     CDS             6355..7065
                     /gene="ispD"
                     /locus_tag="KKO15_002778"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBD6361506.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7065..7544
                     /gene="ispF"
                     /locus_tag="KKO15_002779"
     CDS             7065..7544
                     /gene="ispF"
                     /locus_tag="KKO15_002779"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBD6361507.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7541..8590
                     /gene="truD"
                     /locus_tag="KKO15_002780"
     CDS             7541..8590
                     /gene="truD"
                     /locus_tag="KKO15_002780"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBD6361508.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8571..9332
                     /gene="surE"
                     /locus_tag="KKO15_002781"
     CDS             8571..9332
                     /gene="surE"
                     /locus_tag="KKO15_002781"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBD6361509.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9326..9952
                     /gene="pcm"
                     /locus_tag="KKO15_002782"
     CDS             9326..9952
                     /gene="pcm"
                     /locus_tag="KKO15_002782"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBD6361510.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10092..11231
                     /gene="nlpD"
                     /locus_tag="KKO15_002783"
     CDS             10092..11231
                     /gene="nlpD"
                     /locus_tag="KKO15_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBD6361511.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11294..12287
                     /gene="rpoS"
                     /locus_tag="KKO15_002784"
                     /pseudo
     CDS             11294..12287
                     /gene="rpoS"
                     /locus_tag="KKO15_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(12407..12814)
                     /locus_tag="KKO15_002785"
     CDS             complement(12407..12814)
                     /locus_tag="KKO15_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD6361512.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12961..13554
                     /locus_tag="KKO15_002786"
     CDS             12961..13554
                     /locus_tag="KKO15_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBD6361513.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13554..14501
                     /locus_tag="KKO15_002787"
                     /pseudo
     CDS             13554..14501
                     /locus_tag="KKO15_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14774..15337)
                     /locus_tag="KKO15_002788"
     CDS             complement(14774..15337)
                     /locus_tag="KKO15_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361514.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15502..15753
                     /locus_tag="KKO15_002789"
     CDS             15502..15753
                     /locus_tag="KKO15_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6361515.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15755..17851
                     /locus_tag="KKO15_002790"
     CDS             15755..17851
                     /locus_tag="KKO15_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6361516.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4461 a         4407 c         4835 g         4345 t
ORIGIN      
        1 tcctttaagt ttactgaagg ataaaaatat tgtgactttc tttgatttct ggggaaaaaa
       61 ccgacgtggc gagaaagagg gtatcgacga ctatcacctt ctttgctggc atttgctgga
      121 tgtggccgca atgggctatt taatggttaa aagaaattgc ttcgggctgg ctgattattt
      181 tcgtcaatta gggatttctg acaaggaaca ggcagctcaa tttttcgcct ggttgctgtg
      241 ctggcacgat attggaaaat ttgcccgctc ttttcaacaa ctttacctgc cccctgaatt
      301 caagattcag gaaggtgcca gaaaaaatta cgaaaaaatc tctcattcaa cgctgggcta
      361 ctggttgtgg aatcattatt taagtgaatg tcaggagtta cttccttcat cttcgctctc
      421 tccgcgcaaa cttaggcgag tgatagaaat gtggatgccc gtgacgacag ggcatcatgg
      481 tcggccccct gatcgtatgg atgaattgga taattttctg cctgaagaca aagctgcagc
      541 acgagatttt ctccttgaaa tcaagccact gtttccgcga atagagatcc ccacattctg
      601 ggatgatgac gagggcgttg aacttataaa acaactttcc tggtatatct ctgcaaccgt
      661 cgtacttgca gactggacag ggtcgtcaac gcgatttttt ccacgcgtcg cacaggcaat
      721 ggatattgaa gattactggc aaaaggcttt agttcaggct caaaacgcat taaccgtctt
      781 tccgccacaa gcagaaaccg cgcctttcac cggaattaat acgctgtttc cttttattga
      841 gaatacgaca ccgttacagc aaaaggtgct ggatctggat atcaatcagc caggaccaca
      901 gttgtttatt ctggaagatg tgaccggcgc aggtaaaaca gaagcggcgc ttatcctggc
      961 gcacaggttg atggctgcgg gtaacgctca ggggctgttt tttggcctgc caacaatggc
     1021 aacggccaat gccatgtacg atcgactggt caaaacctgg cttgctttct attcgccaga
     1081 ggtgttcccc gctccagcgg agataaaccg gcgcaatacg gtgcggtttt cccggaagta
     1141 aaccggacgc actggatgcg atgatgggta tcacttagaa ttccccgccc ctgcggtaga
     1201 actcccagct cccattttca aacccatcaa gacgccttcg ccagctcctt caccaacggt
     1261 agcattatcc gcataacgtc acggcagcga cgttctattc ttccaggaag tgccttatca
     1321 atatgttgtt gattatccag tcttacgtca tgccagctat ttcccgccgg gaaggcaggt
     1381 gtttttgcgc gttgctgata accatcctta tttccaagat tccagttagt cgcttccacc
     1441 gaaagtacag caatgcccgc tttgtcgaat atttctgcgt cattacaaca cccagtgcct
     1501 ttcggataat ttttattcaa acccggattg gtcgttgcgg ctattccgtg actgcgtgca
     1561 attgccagcg ccctgtcgcg cgttaatttc cttactgctt caggggtttt tacaccgctg
     1621 ttgaaataca atttatcgcc aacaattaag ttatcgagat taatcaccag cagcgtattt
     1681 ttcttttcgg tgtcactcat ccgcttgagt aaattctcag cgcctaattt tccttcctct
     1741 tcgccgctgg tcgccacaaa tcgaatacca tactcggtag gcgtattttt caggcgttct
     1801 gccagttcca gcatgacacc taaacctgcg gcgttatcat ctattccttg taacgtcagt
     1861 ccgccgagat tggcatctgc gtcagcatcg ctcagcgggg cgtaagtatc cagatgcgcc
     1921 ataatgatga tctgctgcgg cgctttgcct tcatgagcgg caatcaccgt acttcctgtc
     1981 acgttatgcc agttcttgcg attatcgcgg gcggtataaa tataccgact attaaatgtc
     2041 cgaatatcac tgcgataacc catttgctga aactgttggc gaatataatc ggcagataac
     2101 atttctgctg gagttccggt catgcgtccc ggaaagaaag tagcaatatg tcgtgcctgg
     2161 gtattagcaa aatcgccagg tttaggtgac gaggcgtgta cggggagaat aaagcatacg
     2221 ccgagcgcca gggcagcggt acggtggcgc aatgcggaaa acatagtgag tccttaaata
     2281 ccatgcaaat ttttttaccg ccatagtatg aaactgccgc tgcgctaaaa caatttcaaa
     2341 tcttcctaaa cgcccgaaat ccggtgcctt aagcactttt tgatattagc tttgccaaat
     2401 cgttattccg ttaaggaact actcattcta attggtaatt tcattcgttc tcttacgctc
     2461 cctatagtcg aaacatctga tggcaagaaa atagcggtat tgcaaaggaa cggttatgga
     2521 tcaaaaacga cttactcacc tgcggcaact ggaggcggaa agcatccaca ttattcgcga
     2581 ggtggcggca gaattctcaa atccggtgat gctctactct atcggtaaag attccagcgt
     2641 catgctgcat ctggcgcgca aggcgtttta tccaggtacg ctgcctttcc cgttgctgca
     2701 tgtcgatacc ggctggaaat tccgcgagat gtatgagttc cgcgatcgta cggcgaaagc
     2761 ctacggctgc gagctgctgg tgcataaaaa cccggaaggc gtggcgatgg ggattaatcc
     2821 attcgtgcac ggcagcgcga aacataccga tattatgaaa actgaaggcc tgaaacaggc
     2881 gctgaacaaa tacggttttg atgccgcctt cggtggtgcg cgccgtgacg aagagaaatc
     2941 ccgcgctaaa gagcgaatct actctttccg tgaccgcttc catcgctggg atccgaaaaa
     3001 tcagcgcccg gagctgtggc acaactacaa cgggcaaatt aacaaaggcg aaagcatccg
     3061 cgtcttcccg ctctctaact ggaccgagca ggatatctgg caatacatct ggctggaaaa
     3121 tatcgacatt gtgccgctgt atctcgctgc ggaacgtccg gttctggaac gcgacggtat
     3181 gttgatgatg attgatgaca accgtatcga cctgcaaccg ggcgaagtga tcaaaaaacg
     3241 gatggtgcgt ttccgtacgc tgggctgctg gccgctgacc ggtgcggtgg agtcaaatgc
     3301 acaaacactg ccggaaatca tcgaagagat gctggtttcc accaccagtg aacgtcaggg
     3361 tcgcgtgatt gaccgcgacc aggcgggatc catggagctg aaaaaacgtc aggggtattt
     3421 ttaagatgaa caccgtactt gcacaacaaa tcgccaatga aggcggcgtc gaagcctgga
     3481 tgattgcgca acaacataaa agcctgctgc gttttctgac ctgtggtagc gtcgatgacg
     3541 gcaaaagtac cctgattggt cgtctgctgc acgatacccg ccaaatctac gaagatcagc
     3601 tctcatcgct gcataacgac agtaagcgtc acggcaccca gggcgaaaag ctggatctgg
     3661 ctctgctggt ggacggcctg caagctgagc gcgaacaggg catcactatt gatgtggcct
     3721 accgctattt ctctaccgag aagcgtaaat ttattatcgc cgacacccca gggcacgagc
     3781 agtacactcg caatatggcg actggcgcat cgacatgtga actggcgatc ttactgattg
     3841 atgcccgtaa aggcgtgctc gatcaaaccc gtcgtcacag ttttatctcc acactgttgg
     3901 ggatcaaaca tctggtcgtg gcgatcaaca aaatggatct ggtggattac agtgaagaga
     3961 cgttcacccg tattcgtgaa gattatctga cctttgccgg gcagctgccg gataatctgg
     4021 atatccgctt tgtgccgctc tccgcactgg aaggtgacaa cgtggcttcg caaagtgaaa
     4081 gtatgccgtg gtacagcggt ctgacactgc tcgaagtgct ggaaaccgtg gagatccagc
     4141 gagtggtgga tgcccagcca atgcgcttcc cggtgcagta cgttaaccgc ccaaatctcg
     4201 attttcgtgg ctacgccgga atgctggcat ccggtcgcgt ggaagtcggg caacgtgtaa
     4261 aagtgctgcc ctctggtgtg gaatcaaacg tcgcgcggat cgtgactttt gatggtgatc
     4321 gcgaagaagt ctttgccgga gaagcgatca ccctggtgct gacggatgag atcgatatca
     4381 gccgtggtga tctgctgctg gcggcagacg aagcgttacc ggcggtgcag agtgcgtcgg
     4441 tggatgtggt atggatggcg gaacagccgc tttccccggg ccagagttat gacatcaaaa
     4501 ttgccggtaa gaagacgcgt gctcgtgttg atggcattcg ttatcaggtt gatattaata
     4561 accttaccca acgcgaagtt gaaaacctgc cgctgaacgg catcggcctg gtggatctca
     4621 cttttgacga gccactggtg ttagatcgct atcagcaaaa tccggttacc ggtgggctga
     4681 tttttatcga tcgcctgagc aatgtgaccg tgggtgccgg tatggtgcac gagccagtta
     4741 gccaggcaac agctgcgtca tctgaattca gtgcattcga actggaattg aatgcactgg
     4801 ttcgtcgcca ctttccgcac tggggcgcgc gcgatttgct gggggataaa taatggcgct
     4861 gcatgacgaa aacgtcgtct ggcatagtca tccggtcact gtgcaacaac gcgaactaca
     4921 ccacggtcat cgtggtgtag tgctgtggtt taccggcctc tccgggtccg gtaaatcaac
     4981 ggtcgccggg gcgctggagg aggcgttaca taaactcagc gtcagtacgt atctgctgga
     5041 tggcgacaat gttcgccacg gattatgcag cgatctcggt tttagcgatg ccgatcgtaa
     5101 agagaatatc cgtcgcgtcg gtgaagtggc gaatttgatg gttgaagccg gactggtggt
     5161 gctgaccgca tttatctcgc cacaccgcgc cgaacgccag atggttcgcg aacgcgtagg
     5221 agaagggcgc tttattgaag tgtttgtcga tacgccgctg gcgatttgcg aagcccgcga
     5281 tccaaaaggc ttatataaga aagcgcgtgc cggtgaactg cgcaacttta cgggaataga
     5341 ttccgtttac gaagcgcctg aatcggcaga aattcatctc aatggtgaac aattagtaac
     5401 aaatttggta cagcaattat tagatctgtt gagacagaac gatattatca gatcctgaga
     5461 caccaccggg tttccatgcc cggttcgtca aagtaacagg atcaaatatg cgtaatagcc
     5521 ataacattac actaacaaat aacgacagcc ttactgagga tgaagaaact acatggtcac
     5581 tgcctggtgc cgtggtcggt tttatctcct ggttatttgc gctggcgatg ccgatgttga
     5641 tttatggctc taacacgctg ttcttctttc tctacacctg gcctttcttt ctggcactga
     5701 tgcctgtcgc ggtagtggtg gggattgcgc tgcattcatt gatggacgga aagctacgct
     5761 acagtattgt tttcaccctg gtgacagtag gcattatgtt tggcgcactg tttatgtggc
     5821 tactgggcta atttgtactt tcttgtctcc tctgttcata attcaaaccg taactaataa
     5881 tgagattatg ttctgcacgc cctgggtata cgtaacaatg gacaaatgtg gtacatttgc
     5941 ccgcgttgtc gcggtatccc caacagagga tgtagagtcg tcttcggatg catgggatga
     6001 tgatgcagtt tttcaggggg caggatgggt aaactaacgc tgctgttgct ggctattctg
     6061 gtctggctac agtattcgct gtggttcggt aagaacggta tacatgacta tacccgcgtc
     6121 aatgatgatg tggcggcact gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcaa
     6181 ctttttgccg aaattgacga tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt
     6241 aatgaactca gcatgaccag gccgggcgaa actttttatc gtctggtgcc tgacgcgtcg
     6301 aagcgcgcac agtctgcggg gcaaaacaat cgataaatca gcccgggaat taacatggca
     6361 accactcatt tggatgtttg cgccgtggtt ccggcggccg gatttggccg tcgaatgcaa
     6421 acggaatgtc ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg
     6481 catgcgctgc tggcgcatcc ccgggtgaca cgtgtcgtca ttgccataag tcctggcgat
     6541 agccgttttg cacaacttcc tctggcgaat catccgcaaa tcaccgttgt agatggcggt
     6601 gatgagcgtg ccgattccgt gctggcaggt ctgaaagccg ctggcgatgt gcagtgggta
     6661 ttggtgcatg acgccgctcg tccttgtttg catcaggatg acctcgcgcg attgttggcg
     6721 ttgagcgaaa ccagccgcac gggagggatc ctcgccgcac cagtgcgcga tactatgaaa
     6781 cgtgccgaac cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac
     6841 gcgctgacgc cgcaattttt ccctcgtgag ctgttacatg actgtctgac gcgcgctcta
     6901 aatgaaggcg ctaccattac cgacgaagcc tcggcgctgg aatattgcgg atttcatccg
     6961 cagttggtcg aaggccgcgc ggataacatt aaagtcacgc gcccggaaga tttggcactg
     7021 gccgagtttt acctcacccg aaccatccat caggagaata cataatgcga attggacacg
     7081 gttttgacgt acacgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc
     7141 cttacgaaaa aggattgctg gcgcattctg atggcgacgt ggcgcttcat gcgttgaccg
     7201 atgcattgct tggcgcggcg gcgctggggg atatcggcaa actgttcccg gataccgatc
     7261 cggcatttaa aggtgccgac agccgcgagc tgctacgcga agcctggcgt cgtattcagg
     7321 cgaagggtta tacccttggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt
     7381 tgccgcacat tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg
     7441 atgttaacgt gaaagccact actacggaaa aacttggatt taccggacgt ggggaaggga
     7501 ttgcctgtga agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct
     7561 cacttacctc cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt
     7621 tgtggtggtg gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag
     7681 aatcctcaaa aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa
     7741 aattcattcc cgtgaagtca gcttcgctgg gcaaaaagac aaacatgctg taacggaaca
     7801 gtggttatgt gctcgcgtgc cgggcaagga aatgcccgat ctgagcgcct ttcaactgga
     7861 aggctgccag gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gggcgctgaa
     7921 aggtaacgcc tttaccctgg ttctgcgcga agtgagcaat cgcgatgacg ttgaacaacg
     7981 tctgatcgat atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat
     8041 tggcggtagc aacttgcagg gcgcgctgcg ctgggcgcaa accaatactc cggtgcgcga
     8101 tcgcaataaa cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt
     8161 tgctgagcgc ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt
     8221 agccggacgt ggtagctggt ttgtagcaac caccgaagaa ctggcggaat tacagcgtcg
     8281 cgtcaacgat aaagagctga tgataaccgc cgcattgccg ggcagtggcg aatggggaac
     8341 tcagcgtgaa gcgctggcat tcgaacaagc agctgtcgcc gcagaaactg aattacaagc
     8401 tttactggtg cgcgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca
     8461 attaagctgg aactggtggg atgacgtcac cgtagagatc cgtttctggc ttccggcggg
     8521 tagttttgca accagcgttg tcagggaact tatcaacaca acaggtgatt atgcgcatat
     8581 tgctgagtaa tgatgacggg gtacatgcac ccggtataca aacgctggcg aaagccttgc
     8641 gtgagtttgc tgacgttcag gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt
     8701 ctctgacact ggaatcctcc ctgcgcacgt ttacctttga aaatggtgat attgctgtgc
     8761 aaatgggaac cccgaccgat tgtgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc
     8821 cggacattgt tgtgtccgga attaacgccg ggccgaatct gggggatgat gttatttatt
     8881 ccggtacggt agccgccgcg atggaaggcc gtcatttagg ttttccggcg ctggccgtct
     8941 cgcttgacgg gcataaacat tacgacactg ccgcggcggt aacctgttca attttgcgcg
     9001 cactgtgtaa agagccgctg cgcaccggac gtattcttaa tattaacgtt caggatttac
     9061 ccttggatca aatcaaaggt attcgcgtga cgcgctgcgg tacacgacat ccggcagatc
     9121 aggtgatccc gcagcaagat ccgcgcggca atacgctgta ctggattggc ccgccgggcg
     9181 gtaaatgtga tgccggtccg gggaccgatt ttgctgcggt agatgagggc tatgtctcca
     9241 tcacgccgct gcatgtggat ttaactgcgc atagcgcgca agatgtggtt tcagactggt
     9301 taaacagcgt gggagttggc acgcaatggt aagcagacgc gtacaagcac ttctggatca
     9361 attacgtgcg caaggtattc aggatgagca ggtgctgaat gcacttgccg ccgtgccgcg
     9421 tgaaaaattc gttgatgaag cgtttgaaca aaaagcctgg gacaatatcg ccttgccgat
     9481 aggtcagggg cagacaattt cgcagccgta tatggtggcg cgaatgaccg aattactcga
     9541 gctgacgccg cagtcgcggg tgctggaaat tggcactggt tcgggatatc aaacggcaat
     9601 cctggcgcat cttgtccagc atgtttgctc ggttgaacgg attaaaggct tgcagtggca
     9661 ggcacgtcgc cgcctgaaaa atcttgattt acataatgtt tcaacccgtc atggtgatgg
     9721 atggcaaggt tggcaggcac gtgcgccgtt tgacgctatc attgttacgg cggcaccgcc
     9781 ggaaattcca actgcgctaa tgacgcagct ggacgaaggc gggattctcg tcttacccgt
     9841 aggggaggag caccagtatt tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga
     9901 taccgtggag gccgtgcgct ttgtcccttt agtgaagggt gagctggctt aaaacgtgag
     9961 gaaatacctg gatttttcct ggttattttg ccgtaggtca gcgtatcgtg aacatctttt
    10021 ccagtgttca gtagggtgcc ttgcacggta attatgtcac tggttattaa ccaatttttc
    10081 ctgggggata aatgagcgcg ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt
    10141 cactggtttc gctatggctg gcaggctgtt ctgacacttc aaatccaccg gcaccggtca
    10201 gctccgttaa tggcaatgcg cctgcaaata ctaattctgg tatgttgatt acgccgccgc
    10261 cgaaaatggg gacgacgtct acagcgcagc aaccgcaaat tcagccggta cagcagccac
    10321 aaattcaggc tactcaacaa ccgcaaatcc agccagtgca gccagtagct cagcagccgg
    10381 tacagatgga aaacggacgc atcgtctata accgtcagta tgggaacatt ccgaaaggca
    10441 gttatagcgg cagtacctat accgtgaaaa aaggcgacac acttttctat atcgcctgga
    10501 ttactggcaa cgatttccgt gaccttgctc agcgcaacaa tattcaggca ccatacgcgc
    10561 tgaacgttgg tcagaccttg caggtgggta atgcttccgg tacgccaatc actggcggaa
    10621 atgccattac ccaggccgac gcagcagagc aaggagttgt gatcaagcct gcacaaaatt
    10681 ccaccgttgc tgttgcgtcg caaccgacaa ttacgtattc tgagtcttcg ggtgaacaga
    10741 gtgctaacaa aatgttgccg aacaacaagc caactgcgac cacggtcaca gcgcctgtaa
    10801 cggtaccaac agcaagcaca accgagccga ctgtcagcag tacatcaacc agtacgccta
    10861 tctccacctg gcgctggccg actgagggca aagtgatcga aacctttggc gcttctgagg
    10921 ggggcaacaa ggggattgat atcgcaggca gcaaaggaca ggcaattatc gcgaccgcag
    10981 atggccgcgt tgtttatgct ggtaacgcgc tgcgcggcta cggtaatctg attatcatca
    11041 aacataatga tgattacctg agtgcctacg cccataacga cacaatgctg gtccgggaac
    11101 aacaagaagt gaaggcgggg caaaaaatag caactatggg tagcaccgga accagttcaa
    11161 cacgcttgca ttttgaaatt cgttacaagg ggaaatccgt aaacccgctg cgttatttgc
    11221 cgcagcgata aatcggcgga accaggcttt tgcttgaatg ttccgtcaag ggatcacggg
    11281 taggagccac cttatgagtc agaatacgct gaaagttcat gatttaaatg aagatgcgga
    11341 atttgatgag aacggagttg aggtttttga cgaaaaggcc ttagtagaag aggaacccag
    11401 tgataacgat ttggccgaat gaggaactgt tatcgcaggg agccacacag cgtgtgttgg
    11461 acgcgactca gctttacctt ggtgagattg gttattcacc actgttaacg gccgaagaag
    11521 aagtttattt tgcgcgtcgc gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg
    11581 agagtaactt gcgtctggtg gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt
    11641 tgctggacct tatcgaagag ggcaacttgg ggctgatccg cgcggtagag aagtttgacc
    11701 cggaacgtgg tttccgcttc tcaacatacg caacctggtg gattcgccag acgattgaac
    11761 gggcgattat gaaccaaacc cgtactattc gtttgccgat tcacatcgta aaggagctga
    11821 acgtttacct gcgaaccgca cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg
    11881 aagagatcgc agagcaactg gataagccag ttgatgacgt cagccgtatg cttcgtctta
    11941 acgagcgcat tacctcggta gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg
    12001 acatcctggc cgatgaaaaa gagaacggtc cggaagatac cacgcaagat gacgatatga
    12061 agcagagcat cgtcaaatgg ctgttcgagc tgaacgccaa acagcgtgaa gtactggcac
    12121 gtcgattcgg tttgctgggg tatgaagcgg caacactgga agatgtaggt cgtgaaattg
    12181 gcctcacccg tgaacgtgtt cgccagattc aggttgaagg cctgcgccgt ttgcgcgaaa
    12241 tcctgcaaac gcaggggctg aatatcgaag cgctgttccg caagtaagta agcatctgtc
    12301 agaaaggcca gtctcaagcg aggctggcct ttttcttttg tttgggaagc ttgtgttttt
    12361 gtcgcctgga taagacacgt cagcgtccta cccggcaatc agcattctat gtgctcatca
    12421 tcttgcgcac caactgcata aacagctcct gctcttctcc actcaaacgc cccaaaaatt
    12481 cctcatccac gctgtcacca ataggtatcg ccgcagcaag tagcttttct ccttcggcag
    12541 tcagccagac aaagcgtcgc cgcttgtcag cgacgtcatg ttcacgtctg actaagccac
    12601 gattttccat tcttgccaac atttctgcta gcgttgcttt ggtgctgact gctgcttcta
    12661 tcagtgccac ctgttcaata ccaggtttat cagcaatggc gcgcatcacc gcgtactgtg
    12721 gtttggtcag gtcaggtaac tcgtgctgcc agcgagccgt gtgctgctga aaaagctgtc
    12781 gtaactgatg gaacgcttta tttcgtaacg ccatgtaaac tccgggctaa ttctctgtcg
    12841 ctatcatagc ggttttttag tgacgaagaa gatgttgtta cttttcaatc cttgccgctg
    12901 tggaaatgaa ggagtatgtt aataataacg ttcgcatacg aataattaag agaataagca
    12961 atgaaactga tcgtcgggat gacaggggct accggtgcgc ctcttggtgt ggcattactg
    13021 caagcgctgc gtgagatgcc gaatgtcgag actcatctgg tgatgtcgaa gtgggcgaaa
    13081 accaccattg aactggaaac gccttacagc gctcgcgatg ttgctgccct cgcagacttc
    13141 agccataacc cggcggatca ggcggcgatc atctcatccg gttcttttcg taccgacggc
    13201 atgatcgtta ttccgtgcag tatgaaaacg ctcgccggta tccgcgctgg ttacgccgat
    13261 ggcctggtag ggcgcgcggc ggacgtcgtg ctcaaagaag gccgcaaact ggtgctggtg
    13321 ccgcgtgaaa tgccgcttag caccatccat ctcgaaaata tgctcgcact ttcacgcatg
    13381 ggcgtggcga tggtgccgcc gatgcctgcc ttttataacc atcccgaaac ggtagatgac
    13441 attgtccacc atgtggtagc ccgcgtgctg gatcaatttg gcctcgaaca tccccacgcc
    13501 aggcgctggc aaggattacc gcaggcccgg aatttttctc aggagaatga ataatggcat
    13561 ttgatgattt acgcagcttt ttacaggcgc ttgatgacca cggccagtta ctgaaaatca
    13621 gcgaagaagt gaacgctgag ccggatctgg cagcagcagc taacgccacc gggcgtatcg
    13681 gcgacggcgc gcccgcgctg tggtttgata atattcgcgg ctttaccgat gcccgcgtgg
    13741 cgatgaatac catcggttcc tggcagaacc acgcgatttc ccttggcctg ccaccaaaca
    13801 ccccggtcaa aaagcagatt gatgagttta tccgccgctg ggataacttc ccgattgccc
    13861 cggagcgccg cgccaatcca gcctgggcgc agaacaccgt cgatggcgaa gagatcaacc
    13921 tgttcgatat tctgccgctg tttcgcctga atgatggcga tggcggtttc tatctcgaca
    13981 aagcgtgcgt ggtttcccgc gatccgctcg acccggataa cttcggcaag cagaacgtcg
    14041 gcatctaccg catggaagtg aagggcaagc gtaagctcgg cctgcaaccg gtgccgatgc
    14101 acgatatcgc cctgcatctg cataaagcag aagagcgcgg tgaagatctg ccgattgcga
    14161 tcacgctcgg taacgatccg atcatcacgc tgatgggggc cacgccgctg aaatacgatc
    14221 agtctgagta tgaaatggca ggcgcgctgc gtgaaagccc gtacccgatc gccaccgctc
    14281 cgctgaccgg ttttgatgtg ccgtggggtt cagaagtgat cctcgaaggg gttatcgaaa
    14341 gccgtaaacg cgaaatcgaa gggccgttcg gtgagtttac cgggcactac tccggtgggc
    14401 gcaacatgac cgtggtgcgc atcgataaag tctcttatcg cagcaaaccg attttcgaat
    14461 cactctatct cggtatgccg tggaccgaaa tcgactacct atgactaggt aatttattca
    14521 agataaccgg gaagagatta aaaaagacgg gaatagattg gaatcaacaa gttaggaact
    14581 caaggttttc aagataaagc gagtccaaaa atgttaaacg gttcgatatc aataaaacat
    14641 tcaagataac tcacaggttc ttacaatagc ctttaaagac cgttcaaaaa cgattcaggt
    14701 gtttattata gcgctgatgg gtgcaagcgc caaaaccaat gtaattttag cacagctagg
    14761 cacttgcact gggttatgcg ctggctgctt tctgaggtag atttaagtga tcgcccttag
    14821 cttcggacaa cgcgtaaatc tctctaacct tttcgtctgg caaattacca aggcttaacg
    14881 ctatggctct acgaactcca actagagcgt gcaactcttg gatgtgctca tccagaagaa
    14941 tgagtaattg ctccgctcct tttctcccaa gaacatcagc tacttgttgt ttttcttgtg
    15001 gtttaagaaa tgacaagaac attagaattt gagcatcaac attatccgca ttctcttttt
    15061 tgggttcttt ggaatctttt agttcaacat caccctctcc cgtcatgagc cattccaagg
    15121 taacattttc gctttctgct atctttttta actgatgcgg gcgtggtatc ggtgcgtaat
    15181 cccgcctgtt gtaataattt tttaaagtgc cttcattgat cccccatgcc cgagcagccg
    15241 catttcttga agggtatcgt tctactaacc taaagattct ttcgataatc ggttcttttc
    15301 catcggcagg aaaagaacct tggggtgctt ttctcattct tggttccttt ctatctaatt
    15361 cattgataaa taataaaatt aaaaaataaa ccataaaaac accaaagaac cctttacagg
    15421 gaatctttgg tatcttaatc tttatccata cggatatccg accggatatc cgcgcgggta
    15481 attttgtagg ttaaaggaaa gatgagacaa agagaagata gtgaccgcga ttggccttcg
    15541 ctgaagattc agctggcgct caaagataac gggtggtctt ccttccggga gctttcactc
    15601 catgctggtt atgctcagga ttcactgaaa gcggtagttc gcacaccaaa caaaaaatac
    15661 gaacgcgtaa tttctgacgc tataggtgta ccgccagaaa tcatctggcc cagccggtat
    15721 cgtaacgatg gatttatcag gaaggcgtcg tgatatgcag tcgtacgtta ccgtaaatga
    15781 cctgctcggc gtaccaggaa tgccagccac cacaaaaggc atccgacagg cgttacagcg
    15841 tttttctcgt gatcttggtg acgttagtcg ccgtcgggaa ggcacaaaag ccattgagta
    15901 ccacattgat tgtctgcctg aaataacacg aaaggcactg cgtgaacgct atgtggaaca
    15961 gctggtggct acagagaaca atgtttctga agtgaaagct gttaccagaa aaacacgcaa
    16021 tcctgacgct gtccaggcaa tcgaagcata tcgcggttct ccacaactga tggaagaacg
    16081 cctgaatgcg ctgaccgaaa accagcgctg ggtatctgaa gcaagagctg cgctggtggt
    16141 ggaagtgctg aagctggaaa gcgccggtaa ccccgggcga ctgaaagcca ttaactttct
    16201 tgttgaaaaa gcccgtaaag gtgagctgcc ggagcgcctg caacaggccg cagttaacgc
    16261 caatgcaaaa cgtggcgcta atcgtactat cagccgcgat ccactttacc agtgggttct
    16321 gaaatacaac cagtcgcaga acgccgccga acggctgctg ttactggcac cgggaaaacg
    16381 cgacgaaata aaaccggaag aaatcagctg gctgcctgaa tttctggcgc agtatcgcca
    16441 ggtgaacggc agacccatgt cagaagccta cgaggatttt gtcgcagaat ggcagcgtcg
    16501 ccatgcggac gaaccgtaca tgctggaggt gatgccttcc tatgatgttg tccggtatgc
    16561 catgaaaaaa ctgccggaag tggtgaaaca aaaagggcgc gttaccggca gtgaataccg
    16621 tcagcttgaa ggattcaccc gccgcgactg gacagctatg ccggtgaatt atgtctggat
    16681 tggtgacggt cacggtatga agctgaaatg tgcgcatccg atccacgggc gtccattctc
    16741 accggaagtc acgtttgtga tcgatggtgg aacacggttt gttgttggct ggagtcttga
    16801 ccttgccgaa aacgtgttcg ccgttgccgg tgcgatacag cacggcattc gcaaccacgg
    16861 aaaaccgttc ctgtattact cggataacgg ttccggtgaa acggctgaca tgctggacaa
    16921 agaggtcgtg ggtattctgc cacgcctggg gattaaacac cctaccggta ttgccggtaa
    16981 tccacaggga cgcggcatta ttgaacgcct gaaccggaca ctgccgatgc gtatagccag
    17041 gaggtatcgc acctattttg gcaagggtgc tgaccgggaa tcgttgcgtg tacttaaccg
    17101 tgatctgcgt tcagcgttta acgccctgca acaggacaag cctctgaacg accgccagaa
    17161 agcggcaatg cgcgagctgc catcgtgggc ggagcttatt gaggctattc gtgaaggcgt
    17221 ggaatggtac aacaaccgcc cgcattctga gctgccgatg aaaccgaacg gacggcatta
    17281 cagcccgaca gagttcagaa aaaaacgcca ggcagaagaa gacaccgaaa ttgagtggct
    17341 gagtgatctt gagctgcgcg acatgttccg cccaatggtg gaacgaccgg taagacgctg
    17401 cgaaattcag tggctgaaca atatttacta cgcgccggag ttgcgtgacg aacacggtcg
    17461 caaagtgctt atcagttatg acattcatga cgccgagcga attacggtac gtcgcaagga
    17521 cggcagcttt atctgtgagg cgatatggaa cggcaataaa cgcgcagcct ttgctgtcag
    17581 tgctgaatat cacaaacaac agcagcgtat taaaggaatg cgcaaacgcg ctgaggaaaa
    17641 aattcgtgat gctgaagatg aaggtattca gattctggaa cacaaacagg ctgaaccctg
    17701 gctgagtaat gtttatcgcc cggttggtaa tgtggtggca gttcagcaac cggaatatga
    17761 agaagagcat gacgaagaat ttgaacgcga tttccggctg gggatgcaaa aactttttgc
    17821 catgcaggaa gaagatgatc cgctggcctg aataaaaaaa cagtccgcgg gaacggactg
    17881 ttaaataaag gaaacaaagc tgatttgaaa taaccgagga aatactatga ctgatattga
    17941 tgatgtttga ggtgtactgg caatagcgga cactaccatt tgttcttttt ttaagcagcc
    18001 atctgatgat atttttccct gaaggctgcc ggggagatat tccccaga
//
LOCUS       DAECOD010000080        17483 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.080, whole genome shotgun sequence.
ACCESSION   DAECOD010000080 DAECOD010000000
VERSION     DAECOD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.080"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="KKO15_002791"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="KKO15_002791"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBD6361517.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="KKO15_002792"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="KKO15_002792"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBD6361518.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="KKO15_002793"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="KKO15_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBD6361519.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="KKO15_002794"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="KKO15_002794"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBD6361520.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="KKO15_002795"
     CDS             complement(5464..5661)
                     /locus_tag="KKO15_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBD6361521.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="KKO15_002796"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="KKO15_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBD6361522.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="KKO15_002797"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="KKO15_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBD6361523.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="KKO15_002798"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="KKO15_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBD6361524.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="KKO15_002799"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="KKO15_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBD6361525.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="KKO15_002800"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="KKO15_002800"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBD6361526.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="KKO15_002801"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="KKO15_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBD6361527.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="KKO15_002802"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="KKO15_002802"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBD6361528.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLCNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="KKO15_002803"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="KKO15_002803"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBD6361529.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="KKO15_002804"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="KKO15_002804"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBD6361530.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="KKO15_002805"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="KKO15_002805"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBD6361531.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="KKO15_002806"
     CDS             complement(17102..17155)
                     /locus_tag="KKO15_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361532.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17388..17463)
                     /locus_tag="KKO15_002807"
     tRNA            complement(17388..17463)
                     /locus_tag="KKO15_002807"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17428..17430),aa:Gly,seq:gcc)
BASE COUNT         3961 a         4989 c         4393 g         4140 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca cagtccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg ataaccatcc ctttgtaaat aagggcagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcacagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtac
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtaatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaataccatt caccgtgcga agtttctttg aaattaagat
    17281 tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca
    17341 gcaataacgc atcgcgtcgc tgtagatatt ttattgagag aagaatttgg agcgggaaac
    17401 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
    17461 cgcttgggtg gtctgtgcct tac
//
LOCUS       DAECOD010000081        17117 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.081, whole genome shotgun sequence.
ACCESSION   DAECOD010000081 DAECOD010000000
VERSION     DAECOD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.081"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..885)
                     /gene="tdcB"
                     /locus_tag="KKO15_002808"
     CDS             complement(<1..885)
                     /gene="tdcB"
                     /locus_tag="KKO15_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBD6361533.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSG"
     gene            complement(984..1922)
                     /gene="tdcA"
                     /locus_tag="KKO15_002809"
     CDS             complement(984..1922)
                     /gene="tdcA"
                     /locus_tag="KKO15_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBD6361534.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            2112..2456
                     /gene="tdcR"
                     /locus_tag="KKO15_002810"
     CDS             2112..2456
                     /gene="tdcR"
                     /locus_tag="KKO15_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBD6361535.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2712..3251
                     /locus_tag="KKO15_002811"
     CDS             2712..3251
                     /locus_tag="KKO15_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361536.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            3273..3620
                     /locus_tag="KKO15_002812"
     CDS             3273..3620
                     /locus_tag="KKO15_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361537.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            3789..4439
                     /locus_tag="KKO15_002813"
     CDS             3789..4439
                     /locus_tag="KKO15_002813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361538.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(5060..5436)
                     /gene="rnpB"
                     /locus_tag="KKO15_002814"
     ncRNA           complement(5060..5436)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KKO15_002814"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5469..6614)
                     /gene="garK"
                     /locus_tag="KKO15_002815"
     CDS             complement(5469..6614)
                     /gene="garK"
                     /locus_tag="KKO15_002815"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBD6361539.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(6711..7601)
                     /gene="garR"
                     /locus_tag="KKO15_002816"
     CDS             complement(6711..7601)
                     /gene="garR"
                     /locus_tag="KKO15_002816"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBD6361540.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(7631..8401)
                     /gene="garL"
                     /locus_tag="KKO15_002817"
                     /pseudo
     CDS             complement(7631..8401)
                     /gene="garL"
                     /locus_tag="KKO15_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(8417..9751)
                     /gene="garP"
                     /locus_tag="KKO15_002818"
     CDS             complement(8417..9751)
                     /gene="garP"
                     /locus_tag="KKO15_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBD6361541.1"
                     /translation="MILDTVDAKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            10126..11697
                     /gene="garD"
                     /locus_tag="KKO15_002819"
     CDS             10126..11697
                     /gene="garD"
                     /locus_tag="KKO15_002819"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBD6361542.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(11749..12573)
                     /gene="agaR"
                     /locus_tag="KKO15_002820"
     CDS             complement(11749..12573)
                     /gene="agaR"
                     /locus_tag="KKO15_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBD6361543.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            12822..14102
                     /gene="kbaZ"
                     /locus_tag="KKO15_002821"
     CDS             12822..14102
                     /gene="kbaZ"
                     /locus_tag="KKO15_002821"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBD6361544.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            14125..14598
                     /gene="agaV"
                     /locus_tag="KKO15_002822"
     CDS             14125..14598
                     /gene="agaV"
                     /locus_tag="KKO15_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBD6361545.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            14609..15388
                     /locus_tag="KKO15_002823"
     CDS             14609..15388
                     /locus_tag="KKO15_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBD6361546.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            15378..16187
                     /locus_tag="KKO15_002824"
     CDS             15378..16187
                     /locus_tag="KKO15_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBD6361547.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            16274..16708
                     /locus_tag="KKO15_002825"
     CDS             16274..16708
                     /locus_tag="KKO15_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD6361548.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDVLGRCHEECPVEEGI"
     gene            16705..>17117
                     /locus_tag="KKO15_002826"
     CDS             16705..>17117
                     /locus_tag="KKO15_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD6361549.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         4499 a         4304 c         3993 g         4321 t
ORIGIN      
        1 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
       61 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
      121 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
      181 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
      241 gccgtgaacg ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
      301 tgccaccgca ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac
      361 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
      421 atcatcgtaa ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc
      481 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
      541 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
      601 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
      661 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
      721 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
      781 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
      841 aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
      901 cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt
      961 tatgtttaaa taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca
     1021 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
     1081 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
     1141 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
     1201 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
     1261 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
     1321 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
     1381 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
     1441 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
     1501 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
     1561 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
     1621 aatgagactt ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc
     1681 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
     1741 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
     1801 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca
     1861 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
     1921 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca
     1981 ggatatgtgc gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa
     2041 ttaaatatct ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt
     2101 atcaaaaaaa aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg
     2161 ttaatacaaa aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa
     2221 aatttgagtt aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag
     2281 cgataaatac tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat
     2341 tttatcaata ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg
     2401 tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc
     2461 ttctcccctc ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc
     2521 tggtcgccgc gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc
     2581 agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg
     2641 atagtgcgta agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa
     2701 ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa
     2761 tgcacgcagt ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt
     2821 ttgcagaaat tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc
     2881 ggagagtatt atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca
     2941 tgatccgaat ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac
     3001 atgatgatta tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat
     3061 gtacttgctc agccgtcaat aaaaaagagc tggaatgtat taaggagacg atttctaagt
     3121 attgcgcaaa gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa
     3181 cctgcattac ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg
     3241 atttttattg attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg
     3301 atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa
     3361 ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg
     3421 tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa
     3481 aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt
     3541 ttttaggtta taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta
     3601 atgaaccgtt aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa
     3661 aagatattta caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata
     3721 tagttgatct atcctctctg gacttacgta atgtcgattt agattattat gatttcacag
     3781 ataaacatat ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta
     3841 atgccaacat gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg
     3901 attatttact aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa
     3961 aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa
     4021 aaattatgag atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag
     4081 aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca
     4141 gaattaaatt accagagatg aaagaattct catcaaaaaa aatatatgat tacatcgata
     4201 caatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta
     4261 attatactat ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa
     4321 agacgaaaat tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata
     4381 taactcagag aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa
     4441 tgcaatatag acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct
     4501 taccctgtgc accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca
     4561 aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg
     4621 cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc
     4681 aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag
     4741 ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc
     4801 aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa actgaccgat aagccgcttt
     4861 cttttgggta tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
     4921 cgccgaagcg ggtttttacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
     4981 gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
     5041 gcgggttttt acgtaaaaca ggtgaaactg accgataagc cgggttctgt cgtggacagt
     5101 cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg
     5161 ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact
     5221 gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc
     5281 acgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta
     5341 cctggcaccc tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga
     5401 ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg
     5461 cgcccttgtc accccgtgtt gcgcattcca atcgccagtg tcgcggcaat attacgtgaa
     5521 gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc
     5581 aatacgctga agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg
     5641 ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc
     5701 ggtaccttgc cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta
     5761 caatcgtgaa tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc
     5821 agttccgcac caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg
     5881 gggacatctt ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg
     5941 ttattgtcca gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc
     6001 gatgcgccgt tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa
     6061 tcttttaagc gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg
     6121 ccgccaaaac caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc
     6181 atgcctgcgc cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg
     6241 ccgctctcca gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga
     6301 tcgcgttttt ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg
     6361 gttttgccat cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta
     6421 acccaggcgt gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg
     6481 ccaccgtcgg caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct
     6541 ttttctatcg cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg
     6601 attacgattt tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc
     6661 ggcctggatt ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta
     6721 acttcgactt tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct
     6781 aaaccatctg ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc
     6841 gcgccgacgc cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata
     6901 cggaagcccg gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta
     6961 ctgcccgcca gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta
     7021 gttgccagcg ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc
     7081 gccagtttgg tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt
     7141 gctttcatca aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac
     7201 agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg
     7261 cctttcgctt tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata
     7321 tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc
     7381 tctttcacat gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc
     7441 gctttagccg tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg
     7501 ttacggtcag caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta
     7561 cccataatcc ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt
     7621 gcggtggtgg ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc
     7681 cgagatcgct gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg
     7741 cttcgaccgg cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt
     7801 gctgaattgc tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg
     7861 ccagatcgct ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga
     7921 cgttatctac gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact
     7981 gagcgaaata atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc
     8041 gaatgccttc cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt
     8101 ctacaaaagg aatcaggaag ttatagaaac cgatatccag aagacgctta ataatttccg
     8161 gctcgttggt cggcactcac actactggcg cgctggcgct gcctttcaag gccattaact
     8221 gcggaataaa cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa
     8281 acccagccaa accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa
     8341 tttgtacctg tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca
     8401 tcgcttatac ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga
     8461 ggtagcagac catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg
     8521 agtgcagttc acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc
     8581 caaagacgtt aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa
     8641 tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga
     8701 ccaccagcgt ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc
     8761 ccagcacaat cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat
     8821 ccgagaagac acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga
     8881 ccagacccac tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg
     8941 tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca
     9001 tgcggttaga gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc
     9061 ccggcttttt gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt
     9121 cctcagacat acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca
     9181 gcacaaaaca aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca
     9241 gccagccaag cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg
     9301 cggaggcagt accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg
     9361 ggaatgatgg agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg
     9421 cccaggccag cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa
     9481 agaggctgta ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga
     9541 tttgcatcag caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac
     9601 ttaactgcaa ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt
     9661 agttaacggc ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt
     9721 tcctttttgc gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt
     9781 tttattatta tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac
     9841 tcgggaatta gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta
     9901 atacttttta aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt
     9961 tgcgctttac cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt
    10021 ctgcgtttaa actcctgaca ttcttatttc acctaatgaa atcatttatt tttaaatgag
    10081 accaggtcct cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa
    10141 atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca
    10201 attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta
    10261 attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa
    10321 attattcgtt atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg
    10381 atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca
    10441 accaaagtcc cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat
    10501 gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg
    10561 gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg
    10621 aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca
    10681 ccggcagcag ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc
    10741 ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact
    10801 ggaacggatg atgtgcaagc tattccagta gaaatcgcca gcattgtcag tttgcaggat
    10861 gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat
    10921 ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc
    10981 atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat
    11041 gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg
    11101 cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg
    11161 ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc
    11221 aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc
    11281 tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg
    11341 actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa
    11401 caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc
    11461 ctgatggcgg tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt
    11521 gatttaatgg atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg
    11581 ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg
    11641 gatcaatggg gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa
    11701 ttcaattcgt attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga
    11761 cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
    11821 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
    11881 taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
    11941 gcacatccga cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag
    12001 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
    12061 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
    12121 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
    12181 tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag
    12241 gatcacccga tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
    12301 cgcggtgttc agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
    12361 gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
    12421 atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
    12481 actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac
    12541 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
    12601 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat
    12661 tggcgcaagc tactctcgtt tcagtgactt tcattacgtt tcttttgtga atcagatcag
    12721 aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa ttgaaacaaa
    12781 acgaaagatc aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
    12841 tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc
    12901 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
    12961 aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt
    13021 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc
    13081 tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg
    13141 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
    13201 attgcagcat gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac
    13261 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg
    13321 atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa
    13381 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
    13441 cctttgaaaa gcaaggtttg aatgccatct ggccacgcat tattgccctg gtggttcaac
    13501 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct
    13561 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
    13621 aaacgccgca atctctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc
    13681 cggcgctgac cttcgccctg cgtgaagccc ttttttctct ggcggcgatt gaagaagaac
    13741 tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct ggaaaacgtg atgctcgacc
    13801 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
    13861 gttacagcta ctcagatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
    13921 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt
    13981 atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac
    14041 tcattataaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
    14101 aagcaaaaca aagaggaaca tgctatgcca aatattgttt taagccggat tgatgaacgc
    14161 ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg
    14221 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
    14281 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc
    14341 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg
    14401 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
    14461 aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
    14521 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
    14581 ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt
    14641 gggcattatc gcctttatcg ctggcctaga tatgtttaac ggcttaaccc atatgcaccg
    14701 cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt
    14761 aaccggcgat acgctggaac tagtgtggat ggggctggcc ccgctggcgg gcgcacagcc
    14821 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
    14881 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
    14941 cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc
    15001 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
    15061 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
    15121 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
    15181 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacataccct acttcatcct
    15241 gggctttgtt gccgcaacct ggctcaagtt accggtgctg gctattgctg ccgccgcgct
    15301 ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
    15361 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg
    15421 aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcggagc attgccgcgg
    15481 agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
    15541 ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa
    15601 agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
    15661 atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta
    15721 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg cttggcggga
    15781 ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata ggcgcaagcc
    15841 tcgcgctaca aggctctatt cttggcgcag tcgtctttat tgtgctgttc aacgtggtac
    15901 acttggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc
    15961 cccttattaa agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt ggaatgacgg
    16021 taatcggcgc actggtggca acctatgttc gtttaagcac cacgctggaa atcacggcgg
    16081 gcgacgcagt agttaagtta caggctgatg ttatcgacaa actgatgcct gccttcttac
    16141 cgctggtcta caccctgacc atgttctggc tggtacgccg cggctagagt ccgctgcgcc
    16201 tgattgcggt taccgtggtt ctagggatcg tcggtaaatt ctgccatttc ctttaaatac
    16261 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagtggcat
    16321 ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag tttattgcca tcgattttcc
    16381 cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
    16441 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
    16501 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt accggcacca atttgcaact
    16561 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc gtgtacaggc
    16621 gctggagtgt ggacaccgag ggctgaccag tctggtggac gtgttaggcc gctgccatga
    16681 agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
    16741 gaagagggat ggttcgatga ccatcagttg cgtgttgctg acggtgtcat cgcagcaatc
    16801 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc
    16861 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtactc
    16921 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta
    16981 accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
    17041 ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
    17101 aataaaggcg cgcatcc
//
LOCUS       DAECOD010000082        17079 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.082, whole genome shotgun sequence.
ACCESSION   DAECOD010000082 DAECOD010000000
VERSION     DAECOD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.082"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..509
                     /locus_tag="KKO15_002827"
     CDS             <1..509
                     /locus_tag="KKO15_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBD6361550.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="KKO15_002828"
     CDS             complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="KKO15_002828"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBD6361551.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1779..2867
                     /gene="hcxA"
                     /locus_tag="KKO15_002829"
     CDS             1779..2867
                     /gene="hcxA"
                     /locus_tag="KKO15_002829"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBD6361552.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2877..3074)
                     /locus_tag="KKO15_002830"
     CDS             complement(2877..3074)
                     /locus_tag="KKO15_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD6361553.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="KKO15_002831"
     CDS             complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="KKO15_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBD6361554.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5542..5955)
                     /gene="entH"
                     /locus_tag="KKO15_002832"
     CDS             complement(5542..5955)
                     /gene="entH"
                     /locus_tag="KKO15_002832"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBD6361555.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5958..6704)
                     /gene="entA"
                     /locus_tag="KKO15_002833"
     CDS             complement(5958..6704)
                     /gene="entA"
                     /locus_tag="KKO15_002833"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBD6361556.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSLGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6704..7561)
                     /gene="entB"
                     /locus_tag="KKO15_002834"
     CDS             complement(6704..7561)
                     /gene="entB"
                     /locus_tag="KKO15_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBD6361557.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7575..9185)
                     /gene="entE"
                     /locus_tag="KKO15_002835"
     CDS             complement(7575..9185)
                     /gene="entE"
                     /locus_tag="KKO15_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBD6361558.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9195..10370)
                     /gene="entC"
                     /locus_tag="KKO15_002836"
                     /pseudo
     CDS             complement(9195..10370)
                     /gene="entC"
                     /locus_tag="KKO15_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10662..11618
                     /gene="fepB"
                     /locus_tag="KKO15_002837"
     CDS             10662..11618
                     /gene="fepB"
                     /locus_tag="KKO15_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBD6361559.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11622..12854)
                     /gene="entS"
                     /locus_tag="KKO15_002838"
     CDS             complement(11622..12854)
                     /gene="entS"
                     /locus_tag="KKO15_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBD6361560.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12965..13969
                     /gene="fepD"
                     /locus_tag="KKO15_002839"
     CDS             12965..13969
                     /gene="fepD"
                     /locus_tag="KKO15_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBD6361561.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13966..14958
                     /gene="fepG"
                     /locus_tag="KKO15_002840"
     CDS             13966..14958
                     /gene="fepG"
                     /locus_tag="KKO15_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBD6361562.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMCLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14955..15770
                     /gene="fepC"
                     /locus_tag="KKO15_002841"
     CDS             14955..15770
                     /gene="fepC"
                     /locus_tag="KKO15_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD6361563.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15767..16900)
                     /gene="fepE"
                     /locus_tag="KKO15_002842"
                     /pseudo
     CDS             complement(15767..16900)
                     /gene="fepE"
                     /locus_tag="KKO15_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3986 a         4867 c         4446 g         3780 t
ORIGIN      
        1 cctttcgtga aaagccagtg gattgcgttc tgtgggttaa aaacagccaa cttatgccta
       61 atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa aaatccattg
      121 aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat gccatggaaa
      181 tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta aaattacgct
      241 taaaaggtta tttgccggtg acctgtctgg aaggtacgcg taatcaacgc atcgccgcga
      301 ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg gaaatcgtcc
      361 gtgaacttag ccagttggga tgggatgata ataaaattgg caaagagctg ggaatggaca
      421 gcgacgaagt gctgcgactg aagcaaatta acggcctgca agagttgttt gccgaccgtc
      481 aatattcccg cgcctggacg gttaaatagc taaagctggc gcaggcgttc agctgctgcc
      541 agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgtgg gaagggatcg
      601 gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct cctgcgtcag ccactggcaa
      661 aactcaacat catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa gtatgtacct
      721 tcacacggta aaatctccag ccggctttca tttaaagcat tcaccagaat atcgcgcttc
      781 tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag catatcagca
      841 agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac cttgcgaatt
      901 tcggcgctga tgggcgctgg cgcaacacaa taacccactt tccagccggt catatgatag
      961 gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcggatg cgccagcaca
     1021 ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct aatgacaaaa
     1081 atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt
     1141 gcactggggt tatgcggagt gttgaggatc accagtctgg tgcgctcgct taataatgcg
     1201 gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg cttcactatt
     1261 cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact
     1321 tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt cgcccctgcc
     1381 gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc cgttttctga
     1441 gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt tgccccctgg
     1501 gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct
     1561 tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg
     1621 gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat ttttattgta
     1681 cctgacgaag ttgcgtgttg aaggcactat aacacgatgc cagtatttgg caatcaagac
     1741 gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat cctatccgcg
     1801 tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac ctgcacaatt
     1861 ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc gccattgctg
     1921 cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat attttgtttc
     1981 gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt gacgatcgca
     2041 gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc ctcgcccgcc
     2101 gtctcggtct gccatttgtt gccgctccga cgatcgccgc cacctgcgca gcctggacac
     2161 cgctctccgt ctggtataac gatgccggac aggcgctgca ttatgagatt ttcgacgacg
     2221 ccaattttat ggtgctggtg gaaccggaga ttatcctcaa tgcaccgcaa caatatctgc
     2281 tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac
     2341 cagaaacgtt gccgctaacc gtgcggctgg ggatcaataa tgcgcaggcc attcgcgacg
     2401 tcttgttaaa cagtagcgaa caggcacttg ccgatcagca aaatcagcag ttaacgcaat
     2461 cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt ggtctgggcg
     2521 atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact gtcctgccgc
     2581 aaaccgagaa gtttcttcac ggcaccaaag tcgcttacgg aattctggtg caaagcgcct
     2641 tgctgggtca ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt tttcatctgc
     2701 cgactacgct ggcggagctg gaggtggata tcaataatca ggcggagatc gacaaagtga
     2761 ttgcccacac cttgcgcccg gtggagtcca ttcattacct gccagtcacg ctgacaccag
     2821 atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt gcaaatttag
     2881 cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg gaaaaactcc
     2941 tcgtaggtca tcggcgtttg atcgggatga ttaacccgca tatgttcgac atagttgtcg
     3001 taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc ggcttttgcc
     3061 agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc ggatgcggcg
     3121 tgaacgcttt atccggccta caaatgctat caacgcactc ggtcagtccc ctcgccccat
     3181 cggggagagg gttagggtga gggggaaccc tcggcactat gccaacattc gccaacatcc
     3241 cccctcactc tgactttagt gcgcgccttt cgcctgcgct acgatctcct cgacattttc
     3301 cggcatcggc tcatacggcg tttctttcgc cgttggcttc ggatctttca atgccgcaag
     3361 tgccgtctta atcgagaaca gtgccaggac caccacgacc accataaaga agatggttaa
     3421 cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag tatactgcga
     3481 cggaatattg ccgctgtcga tcattgcctg gaacttgtta gcaatggcca ggaagccgac
     3541 tttcgcatcc gggctaaacg ctttctgcca gtcagcggtc agggtacaaa tcagcagcca
     3601 ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga acaacaccac
     3661 ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca
     3721 cagagtgtta ataccgccca gcggatcgac cacgctctga tggaggaagt agccccacgc
     3781 cagtacgcac agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc gcttcaggcc
     3841 aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc
     3901 taccgccgtc agaataaaca gagcttcaaa cagaatggcg aagtgatacc agaacgccac
     3961 atccatcata ccacccagcg caccgtgcag gatgtaagcc atccccaccg ccaacgtcgg
     4021 cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa tctggttgag
     4081 cgtatctggt gtaatgctaa agccccagct actcaccacc tgcgcggcag aagcgactac
     4141 atccgccgtc cctgccggag ccagcaccgc cattgggctg ttcatggcaa aatacacgcc
     4201 cggatcgatg atacaggcag aaactagcgc cataatcgcc acgaaggatt ccatcaacat
     4261 cccaccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc
     4321 ggaagagatc agcgcatgga agccagacac cgcaccacag gcgatggtga taaacaggaa
     4381 cgggaacagg ttaccggtcc ataccgggcc agtgccatcg acaaatttgg tcagcgcagg
     4441 catggtcagc gtcgggcgca taatcaaaat gcctaccgcc agaccgacga tagtcccgat
     4501 tttcaggaag gtcgagagat agtcacgcgg ggccagcagc aaccacaccg gcagcaccgc
     4561 cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta
     4621 cggtgcccag gtcggacttt ctgccaccca gccgccagag ataatggcga aaatcaggaa
     4681 caccaggcca atcactgaca cttcgccaat gcggcccgga cgcagatagc gcaggtagat
     4741 tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg
     4801 agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca tgatcataaa
     4861 gcaggctacc agcgcaatca ccccggcagt tggccccatc tcttctttga ccagctcacc
     4921 cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc
     4981 ggcgagaacc accccggcga gcagccagat catccccggc aggtagccca tttgcgctgc
     5041 cagcaccggc cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt gaccgaacag
     5101 cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat
     5161 acgcgtcggg tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc
     5221 gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg
     5281 atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag
     5341 gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt
     5401 ttgtttctat aacatctttg tacgatttaa caacacggta accatgtaaa tcggagttga
     5461 aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa
     5521 tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca
     5581 acgccgcccc tgttcatcga aaacgacgat ttcccagctc tgattttgcc gcccaagatg
     5641 cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat gggtggcgtt
     5701 aagttccgtg cccaccacac actgcccgtc acgggtcatc ataaatccgg ccatcgatcc
     5761 cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt
     5821 acgggtatca accggcattt cggcttccag cacatcatct cccagacggg tatacacaat
     5881 tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga gcgttaaatg
     5941 gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac aatatcctgt
     6001 agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt
     6061 ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa gccgcgaata
     6121 cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag
     6181 ccaagcgaaa ccacattaca gcgcacgccg ctacccgcca gttccagccc gacgctcaac
     6241 gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat acgcgccgtg
     6301 tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg aaactggttc
     6361 atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt ctgctgccag
     6421 tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgatcagc
     6481 acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcccggcg
     6541 tcggcaacat ccattacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca
     6601 aaacctgtaa ctttcgctcc cgcctcaaca aacgccagcg ccgtggcgta gccgatacct
     6661 ttacctgcgc cggttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg
     6721 agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat
     6781 caccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt
     6841 agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca
     6901 cctcacgcag cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca
     6961 ccacccggcc agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga
     7021 aatcagccag cgcatccgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg
     7081 cggtggtcat acagccaatg tgggcatata ctcctgtaat aatcagctgg ttacgtccac
     7141 tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc cacttcacca
     7201 gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt tccggcgagc
     7261 gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt
     7321 tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgcagcgcag
     7381 caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat
     7441 agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcaactt
     7501 tattctgcgg aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca
     7561 tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta
     7621 tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg atccggtaat
     7681 ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc
     7741 tcttttacca ccagataagc acagcttttt tcgcccatca gctcattttc catgctcacc
     7801 agtgcggcat agatcactgc cggatggctc agcagcaggt tttcgatatc ttcggcagcg
     7861 atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt gatgtacccc
     7921 tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcactg
     7981 gcattgtgct gcggactttt gtaatagcca cggaaggtgt acggcccgcg cgtcatcagg
     8041 cgtccaactt ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg
     8101 tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagacgg
     8161 gtgtagttca ccagcccttc cgccatgcca aacacctgct gcaactggca gccaatctca
     8221 gcgggaatac gcgctgcaag cgtggcagaa agacgtgcgc cgccgacctg taacagtttc
     8281 agcgaggcaa gctgcgcccg gctttcgcct tcagccagcg cctgcaacca caggctgact
     8341 gccggcggca ccagcgcggt aacgttaacc tgatgttttg caatcaacgg gaagcaaagc
     8401 gtggcgctgg gatcggccgc cagaacaacc gttcctccgg caagaaagac gcccagcgat
     8461 cctggcgaac tcatagcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt
     8521 tgtgtgaact gacaaatctc ggcgctacga cgcacgctgt agtaatagtc gttatgagtg
     8581 cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa gctggaaata ggccacttca
     8641 tcagcaggtg atggcgtggc agtaaaaccc tctgccggat ggttaatcgc atcctgcaag
     8701 ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa tggaagaatg ctctgcaaca
     8761 aatgtgttga ggaaatcatc cccgctaaat agcgcatgct ggcgatcggc aatcagtaac
     8821 gccggttcaa tctggctggc gtaggcgttc agttcactac gctgatggct gaacagcgcc
     8881 agcaccggcg cgacgcccag tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg
     8941 ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag
     9001 gcgaggttat ccgccgcctg attcagctcc cgataactca actgccgctc gccgtcgata
     9061 accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc
     9121 cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt gaatggaatg
     9181 ctcatcctcg ctccttaatg caatccaaaa acgttcaaca tggtagaaag tttgacgcct
     9241 gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc ggcaaacaga
     9301 cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc gccgttacct
     9361 tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca
     9421 gcaataacct gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc
     9481 agacaggcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg
     9541 agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta
     9601 ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca gttcatgttc atggcgatct
     9661 ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga
     9721 cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt
     9781 tccgggctgg cccccagcag gacgccacca tcagccagcg ggacatggaa gttgtaacta
     9841 accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg
     9901 gtgatatcaa tcaatcgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg
     9961 gcggcgcggg cacccatctg ttcaaacgtg gtttgttcag gaattgcctg gcgttccacc
    10021 acgttcagcg actggctgcg ggtaaaacag cgggctgagg tctgtttttc ctgacgggag
    10081 aacgactgct aggattcggg aatatacagc gacgaaggct gacgtggatc gaagggaatc
    10141 gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg
    10201 agtttttgct ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaaacgg
    10261 gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc
    10321 gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc
    10381 acaaaatgat aaaggcttta ataatgatta tcacttatat tttcggccgc taacctaaaa
    10441 gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg
    10501 gttgtggtta caatgaaaat gagaagcatt attgatgaat tcgcataagc gcaatgtgat
    10561 gtcctgcgcc gttttgcccc ctctccccaa agggacggat tgtgcgcttt gtcgaatttg
    10621 tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc gccccgctct
    10681 accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta gttcaggccg
    10741 ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc
    10801 agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt gatgctccgg
    10861 tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag ggctttttac
    10921 gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga
    10981 gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg
    11041 attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt
    11101 acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gggcatgaga
    11161 aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa
    11221 tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca
    11281 atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg
    11341 cgaaattgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc
    11401 agcttggtgg ggaaaatctg gctgcggggt taaacggtga atcgctattc ctgttcgctg
    11461 gtgatcagaa agatgccgat gcgatttatg ccaatccact gctcgcgcac ctgcctgcgg
    11521 tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt ccgcctggat tactacagcg
    11581 ccatgcaagt gctggaacgg cttaaggcgc tgttttaagc attaactgtc ggacgctgtc
    11641 acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg
    11701 ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgcg
    11761 cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac
    11821 aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc
    11881 agcaacgaac taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc
    11941 cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc
    12001 atcaataacc ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc
    12061 gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg ccgctgacat ctgccagttg
    12121 tcagccagcg ccggatacag taccagcacc gcgctcgcca tcgtcaataa accacccagc
    12181 agcgggctgg caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcaagc
    12241 ggctgcggtg gcggtggcaa cgccggaagg cttaataacg gtaacaaggt aataaacgtg
    12301 cccgccgccg ccagcccgta gttccaggct acgccgccgg tcgccagcaa taaaccgcca
    12361 atcatgggcg aaatcaccga ccccaggcgc acggtcaaca tggtgatcgc cccggcctgc
    12421 attaagtttt cacgccctac cagtgcagat gttgccgcca gtagcgccgt aacaccaagt
    12481 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc
    12541 agcagtgcat taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    12601 atcacttttt tgcgttcata gcgatccgcc agcacaccgc caaccatcag gccaacaaac
    12661 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg ggtcatcatc
    12721 tggatctgca ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga
    12781 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    12841 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    12901 cacatgttaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    12961 tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    13021 actgattatc gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt
    13081 agtgctggag gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    13141 gcggctgccg cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc
    13201 gttaatgcaa accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc
    13261 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcacagga
    13321 acaactggcg atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttaccgg
    13381 cagtcagggt ggagggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    13441 ggcggtgctg gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    13501 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt
    13561 gctgatcccg gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag
    13621 tttgagcctc ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt
    13681 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat
    13741 tgcctttatc ggcctgatga tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg
    13801 ctggtcgctg cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat
    13861 cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc
    13921 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct
    13981 cgccgattac tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg
    14041 ggattacgca gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gttgatgggc
    14101 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    14161 gcgctgttga ttggcgcggc tctgggcgtc agcggcgcga tttttcagtc gctgatgcgt
    14221 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg
    14281 gtggcgatgg tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggtgggc
    14341 ggcattatca cttcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt
    14401 cggttgatta ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg
    14461 ttgctgaaag cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc
    14521 aacggcctga cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt
    14581 gccgccacct tgctggtgcg gcgaatgtgc ttgctggaga tgggcgatga taccgcctgc
    14641 gcgctgggcg tcagcgtcga gcgttcgcgt ctgttaatga tgctggttgc agtggtgctt
    14701 accgctgcgg caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac
    14761 attgcccgac gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga
    14821 gcgctgttac tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt
    14881 ccggttggcg tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag
    14941 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg
    15001 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca
    15061 cggcaattat cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc
    15121 tgatgacgcc tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca
    15181 gtaaagaagt tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata
    15241 tcaccgtgca ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct
    15301 ggcgcaaaga ggatgaagaa gcggtaacga aagcgatgca ggccacggga ataactcatc
    15361 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatcgcga
    15421 tggtgctggc ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata
    15481 tcagtcatca gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata
    15541 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    15601 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    15661 tgattgagcg catttatggt ctgcgatgca tgatcattga cgatccggta gccggaatgc
    15721 cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt
    15781 ctgccatcat ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac
    15841 aagccaccat cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt
    15901 cttttttcac tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt
    15961 tgacatttgc ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca
    16021 gttccgcaac gtcagtaacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc
    16081 cgagagaaat agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct
    16141 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    16201 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    16261 tgatctccag tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat
    16321 aatcgatata cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta
    16381 gcgtccagga ggtatacagt gacggttcat cttttttctt actagcattg tcatcaaccg
    16441 ctttcatttt ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg
    16501 cctcttttaa ttagtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa
    16561 cagactgaaa cttcttgata aacaggttaa atgcaccgcc acggtcgata ttaacatcga
    16621 gatcaagaac gcggagtttg gtaaaggttt tctccaggtc ctgccactga atggcttcgg
    16681 cgggagtaat aacggctgag ctggtccatt tttgcggcag gatgaaagag atcagtaaac
    16741 ctgcgcaggc aaatgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt
    16801 cgattagatt aagtaaatca atttcattat tactgggcga cgccagagga tactcagaaa
    16861 aatgagcgtc acttccctgt ttaatattca atgatgacat ggggtataac ctgaaaatga
    16921 gtccaatagg ggaaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag
    16981 ctgaagtttg taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc
    17041 atcaaaccta taaatcaaac aaataaaaaa cccataatt
//
LOCUS       DAECOD010000083        16769 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.083, whole genome shotgun sequence.
ACCESSION   DAECOD010000083 DAECOD010000000
VERSION     DAECOD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.083"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..852
                     /locus_tag="KKO15_002843"
                     /pseudo
     CDS             1..852
                     /locus_tag="KKO15_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            825..2342
                     /locus_tag="KKO15_002844"
                     /pseudo
     CDS             825..2342
                     /locus_tag="KKO15_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2342..2995
                     /locus_tag="KKO15_002845"
     CDS             2342..2995
                     /locus_tag="KKO15_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD6361564.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3062..3586
                     /locus_tag="KKO15_002846"
     CDS             3062..3586
                     /locus_tag="KKO15_002846"
                     /inference="COORDINATES: protein motif:HMM:NF012790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361565.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAA"
     gene            3650..4369
                     /locus_tag="KKO15_002847"
     CDS             3650..4369
                     /locus_tag="KKO15_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBD6361566.1"
                     /translation="MESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAGARVA
                     QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQ
                     ARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFYNP
                     DGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRASETFMYARAMGWKNVSVYDGGWY
                     EWSSDPKNPVATGERGPGSSK"
     gene            complement(4378..4998)
                     /locus_tag="KKO15_002848"
     CDS             complement(4378..4998)
                     /locus_tag="KKO15_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD6361567.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLTFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            5085..5492
                     /gene="nudG"
                     /locus_tag="KKO15_002849"
     CDS             5085..5492
                     /gene="nudG"
                     /locus_tag="KKO15_002849"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBD6361568.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5458..5730)
                     /locus_tag="KKO15_002850"
     CDS             complement(5458..5730)
                     /locus_tag="KKO15_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBD6361569.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQRCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5966..7305
                     /gene="gdhA"
                     /locus_tag="KKO15_002851"
                     /pseudo
     CDS             5966..7305
                     /gene="gdhA"
                     /locus_tag="KKO15_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            complement(7422..8462)
                     /locus_tag="KKO15_002852"
     CDS             complement(7422..8462)
                     /locus_tag="KKO15_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6361570.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8589..10550)
                     /gene="topB"
                     /locus_tag="KKO15_002853"
     CDS             complement(8589..10550)
                     /gene="topB"
                     /locus_tag="KKO15_002853"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBD6361571.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(10555..11598)
                     /gene="selD"
                     /locus_tag="KKO15_002854"
     CDS             complement(10555..11598)
                     /gene="selD"
                     /locus_tag="KKO15_002854"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBD6361572.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11715..12266)
                     /locus_tag="KKO15_002855"
     CDS             complement(11715..12266)
                     /locus_tag="KKO15_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBD6361573.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12427..14283
                     /gene="sppA"
                     /locus_tag="KKO15_002856"
     CDS             12427..14283
                     /gene="sppA"
                     /locus_tag="KKO15_002856"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBD6361574.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14450..15466
                     /gene="ansA"
                     /locus_tag="KKO15_002857"
     CDS             14450..15466
                     /gene="ansA"
                     /locus_tag="KKO15_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBD6361575.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15477..16118
                     /gene="pncA"
                     /locus_tag="KKO15_002858"
     CDS             15477..16118
                     /gene="pncA"
                     /locus_tag="KKO15_002858"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBD6361576.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16211..>16769)
                     /locus_tag="KKO15_002859"
     CDS             complement(16211..>16769)
                     /locus_tag="KKO15_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361577.1"
                     /translation="TVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPV
                     GIFIAALIIDHFPRRLFGSALLIIIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCF
                     ASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLV
                     LCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         4006 a         4409 c         4307 g         4047 t
ORIGIN      
        1 accttaaaag aggccaattt attacaaacg ggctgggcgg agactctgcc caactggcgc
       61 tatgtcgaca cacagctgcc ggtgcgggga gatttttcag tgccgacaca aggtgcggaa
      121 tcgccctggg gcggcgcaca actgacgttt atcgcccgcc gcgatgttac gccacagcca
      181 ccacaaacgc cgcaagcctt actggagttt gctaaagcca atcccggcac ggttacttat
      241 ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat tatgctgacg
      301 ccagatcccg ccgcattaaa agaagcgccg gacgatgcga ctttcgcccg tgtcactgcg
      361 cccttgtagc aatatcttga tgcactgcat ccgtatttgt ggcgcgaagg aaaggatttc
      421 ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gcacattgcg cctgtcgctg
      481 acctttaacc ccgcacatgc gcagcaaaaa atcgccagcg gcgatttgcc ggcaagcagt
      541 tacagttttg gctttcgcga ggggatgatt ggcaacgtgc atttcgtcac cattccagcc
      601 aacgcgaatg ccagtgctgc ggcgaaggta gttgccaatt tcctgctctc acccgatgcg
      661 caactgcgta aagcagatcc cgttgtctgg ggcgatcctt ctgttctcga tccgcaaaaa
      721 ctgcctgacg ggcagcgcga aatattgcaa tcaagaatgc cgcaggatct gccgccggta
      781 ctggctgaac cgcacgcagg atgggtgaat gcactggaac aagaatggtt acgccgttac
      841 ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca ccgctgatcc
      901 cggcagctct cacgctgata tcgcctgcgt tgtcgttgac acgctggcag gcgttatttg
      961 ccgatccaca gttaccgcag gcattactgg caacgctggt gtcgacaacc atcgcggcgg
     1021 tcggggcatt gttgattgcc ctgctggtga ttgtggcgct atggcctggg ccgaaatggc
     1081 agcgtatgtg cgcccgtctg ccgtggctgc tcgccattcc ccatgtggct tttgccacca
     1141 gcgccctgct gctctttgct gacggagggc tgctatatga ctatttcccg tatttcactc
     1201 cgccaatgga ccgatttgga tcggtctggg cctcaccctt gcagtgaaag aaagcgcatt
     1261 tctgctgtgg atcttagtgg cagtattgag cgaaaaacgg ctgttgcagc aggtcattgt
     1321 gctggattcg ctgggctaca gccgctggca atgcctgaac tggctgctgt tgccctccgt
     1381 cgcgccttcg ctggcaatgg cgatgctggc gattgttgcc tggtcgcttt cggtcgtgga
     1441 tgtggcgatt attcttgggc caggtaatcc accgacgctg gcagtaatta gctggcagtg
     1501 gttaactcag ggcgacgcgg atcaacaaac gaaaggcgca ctcgccagtc tgctgctgat
     1561 gctgttactc gccgcctacg ttttggcgca gctggcggcg cactattccc cgcgtagatg
     1621 gcattcgcaa gcctgccaca cctttattac caggcactac gctggcgagt tttttaccct
     1681 taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg acgatcaata
     1741 gtgaagcact catcaacagt ctgacaatgg ggctggtagc aacattcatc gctttgctcc
     1801 tgttactgct gtggctggaa tggggggcac agcgtcgcca gttgtggcta tggttgccca
     1861 ttctattgcc cgctctgcca ctggtggcgg gccagtacac gctggcgcta tggctgaacc
     1921 tggatggaag ctggacggcg gtggtctggg ggcatctgct gtgggtaatg ccgtggatgc
     1981 tgtttatcct gcaacccgcc tggcagcgca ttgattcacg gttaattttg attgcgcaaa
     2041 cactgggctg gtcgcgggcc aaaatcttct tttacgtgaa atgcccactc atgttgcgcc
     2101 ctgtgctgac tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat atgccgacgc
     2161 tgtggctggg cgcggggcgt tttccgacgc tcaccactga ggcggtggca ttaagcagcg
     2221 gcggcagcaa cggtattctc gccgcccagg cgttatggca actgttgtta ccgcttatta
     2281 tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa ggaatccgct
     2341 aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc tgacaaacgt
     2401 taactttacg gttgataaag gtgacatcgt cacgttaatg gggccgtctg gctgtggaaa
     2461 atccactctg ttttcatgga tgattggtgc actggccgga cagttttctt gtacaggtga
     2521 gctatggctc aatgagcaac ggattgacat gctacccacc gcacagcgtc agattggcat
     2581 tctttttcag gatgcactgt tatttgacca gttcagcgtc gggcaaaatt tactgctggc
     2641 gctaccggcg acacttaagg ggaatgcccg acgtaatgcc gtgaatgatg cacttgagcg
     2701 ttcaggcctt gacggagctt tccatcagga tcctgccact ttgtctggcg gtcagcgagc
     2761 gcgcgttgct ctactacgcg cccttctcgc ccagccaaaa gcgttactcc tggatgagcc
     2821 attcagccgt cttgatgtgg ctctgcgcga taattttcgc cagtgggtgt tcagcgaagt
     2881 tcgcgccctg gcgatccccg tcgttcaggt aacgcacgat ctccaggatg ttcctgctga
     2941 tagttctgtt ctggatatgg cgcagtggtc agaaaattac aacaaactgc gataacgcaa
     3001 agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa actacaatgt cgggattttc
     3061 gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg cttgcgcttc
     3121 ttcgtgggcc gctgaactgg cgaagcctct tacacttgac caacttcagc aacaaaatgg
     3181 caaagcgata gatactcgcc ccagcgcgtt ttataacggc tggccacaaa ccttaaatgg
     3241 cccttctggt catgaacctg ccgccttaaa cctctctgct agctggctcg acaaaatgag
     3301 caccgaacag ctcaacgcgt ggatcaagca acataacctg aaagccgatg ccccggtggc
     3361 gctgtacggt aatggcaaag atgtcgacgc cgtcaaaacg cgactgcaaa aagcaggttt
     3421 tacgcatatc tccattctga gtgacgcgct aagcgaacct tcccgtctac aaaaactgcc
     3481 gcactttgag cagctggttt atccgcaatg gctgcatgac ctgcaacaag gtaaagaggt
     3541 tacggcgaaa cctgtcggtg actggaaagt cattgaagcg gcctagggcg ctcctaagct
     3601 ttaccttatc agccatattc ctggcgctga ctacatcgat accaacgaag tggaaagcga
     3661 accgctgtgg aacaaagttt ctgatgaaca actaaaagcg atgctggcaa aacacggcat
     3721 tcgccatgac accacggtca ttctgtacgg gcgtgacgta tacgctggag cgcgtgtggc
     3781 gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg ctggatggcg gctggcaaac
     3841 ctggtccgac gcgggactgc ctgttgagcg aggaacgcca ccgaaagtga aagcggaacc
     3901 ggatttcggc gtgaagatcc cggcacaacc gcagttgatg ctcgatatgg aacaggcccg
     3961 tggactgctg catcgccagg atgcatcgct ggtgagcatt cgttcgtggc cagaatttat
     4021 cggtacgacc agcggttaca gctatattaa accaaaaggt gaaatagccg gagcacgttg
     4081 gggacacgct ggtagcgact cgacgcatat ggaagatttc tataacccgg atggcaccat
     4141 gcgtagcgcc gatgatatta ccgctatgtg gaaagcatgg aatatcaaac cagatcagca
     4201 agtttcattc tactgcggca ccggctggcg cgcgtccgaa acctttatgt acgcacgcgc
     4261 catgggttgg aagaatgttt ccgtgtatga cggcggctgg tacgaatgga gcagcgatcc
     4321 aaaaaatccg gttgcaaccg gtgaacgcgg cccgggcagt agcaaataac attgcaatta
     4381 ctgacgctgg agcgacttca gcgtcagata cccactccac acccgcgtaa aggtcgtcat
     4441 ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac catgcaggga ataaacatcc
     4501 cagcacaaac agtaagatcg tttctgtgcc ttcggttaat ccccccagat agtaaaacga
     4561 cttatgtgca taaccggggt tatcaatctg atgttttgct gccagtgcag caaaggtgag
     4621 aaaactgctg cccgtgccga taaacgcaaa caacaaccag ccgcctgcca gcgcattttg
     4681 ctccggtgca gcaagaataa agccaaacgg caccagcgcg taaaagagaa aatcgagaga
     4741 aatatcaaga aagccgcccg cgtcggtaag ccctctgcgc cgcgccagcg caccgtccag
     4801 accatcaagc agcctgttca acaaaatgac gactagcgcc gccagatacc agcccagcgc
     4861 cagaaacggc agagccagca cgccgatggc aaatcccact aatgttaaac catctggcgt
     4921 aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa tccggggatg
     4981 aagatggcgg tctagcacag gcactcctta aatataaagc ctttctgatt gagcaacagt
     5041 gcggatatta tggcattttt cgcttatctg cccgtgtgta atttatgaaa atgattgaag
     5101 ttgttgccgc catcattgaa cgtgatggca aaattttact cgcgcaacgc cccgcccaga
     5161 gcgatcaggc gggattatgg gagtttgccg gaggtaaagt cgagctggat gaaagccagc
     5221 agcaggtgct ggtgcgtgag ttaaacgaag aattggacat cgaagcaact gtgggtgagt
     5281 atgttgccag ccatcagcga gaagtttcgg gccggattat ccatcttcat gcctggcacg
     5341 tacccgactt ccacgggacg ttacaggcac atgaacatca ggcgctggtc tggtgctcac
     5401 ctgaagaggc gctgcaatat ccgctggccc ctgctgacat tccattatta gaggcgttta
     5461 tggctttacg cgccgccaga gcagcggatt agtgctaagg gttttgtcat cacgctggca
     5521 ttgcagcagt attccttcgg ctttaattac cgccccttca gaataatttt gatcctgata
     5581 aacgcagcgc tgagtacagg gctgcgctga ctgcccgcct gaactgaaca cttctggcgg
     5641 tacgtttacc tccacgtccg gacgataatg cgggttagcc agtgcaatta atggaaatgc
     5701 taatactacg gcgaacaatg ctcgactcac agggaactcc ttaacgttat tgtctctgct
     5761 actgataacg gtagccgggt ggcaaaactt tagcgtctga gttatcgcat ttggttatga
     5821 gattactctc gttattaatt tgctttcctg ggtcattttt ttcttgctta ccgtcacatt
     5881 cttgatggta tagtcgaaaa ctgcaaaagc acatgacata aacaacataa gcacaatcgt
     5941 attaatatat aaggttttta tatctatgga tcagacatat tctctggagt cattcctcaa
     6001 ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc gtgaagtaat
     6061 gaccacactc tggccttttc ttgaacaaaa tccaaaatat cgccagatgt cattactgga
     6121 gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg atgatcgcaa
     6181 ccagatacag gtcaaccgtg catggcgtgt gcagttcagc tctgccatcg gcccgtacaa
     6241 aggcggtatg cgcttccatc cgtcagttaa cctttccatt ctcaaattcc tcggctttga
     6301 acaaaccttc aaaaatgccc tgactaccct gccgatgggc ggtggtaaag gcggcagcga
     6361 tttcgatccg aaaggaaaaa gcgaaggtaa agtgatgcgt ttttgccaga cgctgatgac
     6421 tgaactgtat cgccacctgg gcgcggatac cgacgttccg gcaggtgata tcggcgttgg
     6481 tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag ctctccaaca ataccgcctg
     6541 cgtcttcacc ggtaagggcc tttcatttgg cggtagtctt attcgcaagc taccggctac
     6601 ggtctggttt atttcacaga agcaatgtta aaacgccacg gtatgggttt tgaagggatg
     6661 agcgtttccg tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa
     6721 tttggtgctc gtgtgatcac tgcgtcagac tccagcggca ctgtagttga tgaaagcgga
     6781 ttcacgaaag agaaactggc acgtcttatc gaaatcaaag ccagccgcga tggtcgagtg
     6841 gcagattacg ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctgta
     6901 ccggttgata tcgccctgcc ttgcgccacc cagaacgaac tggatgttga cgccgcgcat
     6961 cagcttatcg ccaatggcgt taaagccgtc gctgaagggg caaatatgcc gaccaccatc
     7021 gaagcgactg aactgttcca gcaggcaggc gtactgtttg caccgggtaa agcggctaat
     7081 gctggcggtg tagcaacgtc gggcctggaa atggcacaaa acgctgcgcg cctgggctgg
     7141 aaagccgaga aagttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt
     7201 gttgagcatg gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa cattgccggt
     7261 tttgtgaagg ttgccgatgc gatgctggcg cagggtgtga tttaagttgt aaatgcctga
     7321 tggcgctacg cttatcaggc ctacagatgg gcacaattca ttgcagttac gctctaatgt
     7381 aggccggtca agcgcagcgc ccccggcaaa atttcaggcg tttatgagta tttaacggat
     7441 gatgctcccc agggaacatt tcttatgggc caacggcatt tcttactgta gtgctcccaa
     7501 aactgcttgt cgtaacgata acacgcttca agttcagcat ccgttaactt tctgcgatag
     7561 cagcagatat gccagtaaag aaatcccatt agactatttt tttgataatc ttcttcgctt
     7621 tcgaacaact cgtgcgcctt tcgagaagca agcattatat aatgccaggc cagttcttct
     7681 tcaattgtcc cgttttgaaa agctgtgctt gatatcgaga tcatccatga taattccgcc
     7741 gcccatatta gcttcgccga ggatttaccg gagcgattat tagcgcaatc agagatatag
     7801 tctgagggaa aaacagcaaa tttattcaac aaggcgataa cctgctctgg ggcttcctcc
     7861 atgtttgctt taaaggtatt ggctccatgg tcgccagaaa gaaaatgctc cattaaggca
     7921 caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg tcttttactg
     7981 atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt gccaggttca
     8041 ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa tcgataaggt
     8101 gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc tttagcctga
     8161 taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcata
     8221 attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt caaaccgttg
     8281 gatgttatcg ctgcgaatac aaacattaca gcgacaacac atgttaaata aaacccccac
     8341 ttacaaagca gcatggcctt attttcttta atcacccgtt caaaattact attaaatatt
     8401 ccccagccat taaaagaata cttctcgctc ccaggatggt tttgcaataa aacttttttc
     8461 atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaatat
     8521 ccaacgaatt ttcttgatcc gctggatatt tcagaatatg actcgaatag cacgaaagat
     8581 tcgctcgctt acgctatcgc cccgcttccg gcttcatctg ctggcggact ttttttcgca
     8641 ctacgtttac gcggtgcagc ctttttctta tcagcactgc caccactgcc cggagccaca
     8701 atgccgcgaa actgccgcac cggcgtacgt ttggcttgat caataagctg atatagcgtc
     8761 cccaccagcg gctgcataaa gtcctgatag cgacactgct tttcgctgat ttgcgtcagc
     8821 accgattccc agtgcgcggt catgtccggt cgcgtcgcca tttctggcag cgaatggaat
     8881 aacgcttttc cggcgtcggt ggagtggata tagcgccctt ttttggtcag aaaaccacgc
     8941 ttgaacagca gttcaataat cccggcacgg gttgcttctg tccccagacc atcggtcgca
     9001 cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg cgatcccggt catcgccgaa
     9061 agcagtgttg catcggtaaa atggcgcggc ggctgggttt gccgctctac cacttcacct
     9121 ttttcacaca gcaactcatc gcctttcgcc accacaggca atggcgtgcc gtcgttttct
     9181 tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg cttcagcaag aaaacgggct
     9241 ttagcgacaa atttgccttt ggcaatgtcc agttcgataa cacacttgcg gaacaccgca
     9301 tccgggcaga actgcatcag gtattgacgg gcaatcaggt tatagacctt cgcttcgttc
     9361 tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga tggcgtggtg cgcatcgacc
     9421 tttttgtcat cccaacagcg gttgcgtata tctggatcta ccactggctg cggcaacaga
     9481 tccggtgcat gaacgctgat ggcattcatc accgcgtggc gtccggcaaa atgttcttct
     9541 ggcaaatagc gacaatcgga acgcggataa gtgattagct tgtgcgtttc gtacagcttc
     9601 tggcagatat caagcacgtt ctgcgcgctc agaccgaagc gttttgccgc ttcaatctgc
     9661 aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt cccgtttatc gttatagctg
     9721 gtgacaatag ccggttgacc actaatgcgg ttaaccacat gctccgccag tggacgatgt
     9781 aacaagcgcc cttcttcatc ctggtacggt tcacacgctt cgctcggttg ccagatagcg
     9841 gtaaaccgct catcggcagg tgtcacgata tgtgctttga cttcaaagaa gtctttcgcc
     9901 acgaagtttt caatctcttc atcgcggcgc accaccagcc cgagcacggg tgtctgcacg
     9961 cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac cgagaatggt atacgcacgg
    10021 gtcatattga tgccgtacag ccagtcggca cgcgctcgcg ccagcgcaga aacgcacaat
    10081 ggcacaaact cactgttgga acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc
    10141 aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt ccggtgccag ttgcagatag
    10201 tccagcactt catccaccag caattgccct tcacgatccg ggtccccggc gtgaacgatt
    10261 tcgctggctt catgcaggaa gcgtttgatg acgttaagtt gtttggtcac ggagggtcgt
    10321 ggctgtaatt gccacttttc cgggacaatc ggcaaatccg caagattcca gcgcgcatag
    10381 cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt gaccgataca ccaagtcacc
    10441 acctgaccat taccgcactc gataaagcca tcgcctttcc ggtgcggttt gggcaggaca
    10501 tcagcaatgg cgcgcgccag actcggtttt tcggcaataa acaaccgcat taaattaacg
    10561 gatctcaacc atggcacgac cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc
    10621 aatgccaaac tcggcggctg tagttttaac ctcattttct gcttccggca tgaccgccag
    10681 cagcaaaccg ccagaagttt gcggatcgca cagcagatcg cgcacttcac gcggcatttc
    10741 acccatcaga tgaccgtagc tggcaaagtt acgttcagtg ccgccaggta ctgcgcccaa
    10801 cttaatgtac tcttcaacac cggggagttt cgggatcgct tcatagtcga cgcgtgcctg
    10861 cacaccagcc ccctgacaca tttcgctcaa gtggcccagc agaccaaagc ccgtaacgtc
    10921 ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg cctgcgatgt tcatccggca
    10981 catcacttcc gtcgccagtc cctgatgttc tggtttcaac agtgattttt tctcagccgt
    11041 ggtaagaacg ccgatcccca gcggtttcgt caggaacagt ttgcatccgg cttgtgcggt
    11101 actgtttttc ttcacccgct cggtcggtac gatccccgtt accgccagac caaaaatcgg
    11161 ctccggcgca tcgatggagt gaccgccagc cagcgcaata cccgcctgac ggcatgcata
    11221 gcgtccacct tcggttactt cgcgggcaat ttccggggaa agtttgttaa tcggccagcc
    11281 gaggatcgca atcgccataa tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt
    11341 agtcgccgca atgcggccaa aatcgaaagg attatcaacg atcggcataa agaagtcggt
    11401 ggtactgata acgctggtgc cattgcccag atcgtacacc gccgcatcgt cgcgggtttc
    11461 attacccaca agcaaattcg gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc
    11521 caacactttt ggggaaattt tacagccgca accagctccg tggctgtatt gggtcaaacg
    11581 aatcgagttc tcgctcatgg acatctcctg tcaatgcaat ccgggtatgg taaccctcat
    11641 tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc tttgctcaca atccagacag
    11701 tttcgcgaca attatcagaa ataagttaca aacggcgtcg ggtccgggac gttaatcgac
    11761 gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac cgacaatttt atcctgctca
    11821 cggcaaccga atgcttcacg cactaccgga ctttcagtta atgcgccact gcgccagatg
    11881 ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca tgaccgcgca tccggcagac
    11941 atttcctgtt cccatcgcgg gactttatga ttttctttgc atttcgccac caccgtgatg
    12001 atgagcggtg cgcggaacgg cgcattacgg gctttgtcga tagctttatc atcactaccg
    12061 gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct cgcacccttc cccttcaatc
    12121 acaaaaaaat gccacggttg catggactta tggtccggcg cacgcatacc cgcacgcagg
    12181 atgttttgca gttgttcacc cgttggcgcg ggttcagcca ggcgggaggc gctacggcga
    12241 ttgatcaata gttcgagtgc atccatttga ttaactcctg tcgtgatatt tattcacaaa
    12301 attaacacga gagtggattt tgttacagca cagtccgcaa ttcctgctga caagtgccgg
    12361 ttgggtcatt acgataacca catctattgc gcctgtgaca ggtgtgacct taagttggga
    12421 gaatacatgc gaaccctttg gcgatttatt gccggatttt ttaaatggac gtggcgtctg
    12481 ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta ttttcctcgt actggttggt
    12541 gtggggatct ggatgcaggt cagtggtggt gattcgaaag aaacggccag tcgtggcgca
    12601 ctgctgctgg acatttctgg tgtgatcgtc gataaaccag acagttctca gcggtttagt
    12661 aaattaagcc gccagctgct tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt
    12721 gatatcgtca acaccattcg ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg
    12781 gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc agtacatcgg caaagccctg
    12841 aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg gcgagaacta cagccagggg
    12901 caatattatc tcgccagttt cgccaataaa atttggctgt caccgcaagg cgtggttgat
    12961 ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt tgctggataa gctgaaagtt
    13021 tccacccatg tgttccgcgt gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat
    13081 gatatgtcac cggcagcccg cgaagctgac agccgctgga ttggtgagct gtggcaaaac
    13141 tatctgaata ctgttgccgc taaccggcag atccctgctc agcaggtatt ccctggcgcg
    13201 caagggttgc ttgagggttt aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa
    13261 aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg aaaaagctct gaccaaagag
    13321 ttcggctgga gtaagactga taaaaattat cgcgccatca gttattacga ttacgcattg
    13381 aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg
    13441 gatggcgagg aaactcaggg gaatgttggc ggtgatacca ctgcggcaca aatccgcgat
    13501 gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc
    13561 gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag cccgggcagc gggtaagtct
    13621 gtggttgtat cgatgggcgg catggcggca tctggtggtt actggatttc cacgccagct
    13681 aattacattg tggctaaccc cagcaccctg accggttcta tcggtatctt cggcgtgatc
    13741 accaccgtag aaaatagtct ggattcgatt ggtgttcata ctgatggtgt ctcaacttca
    13801 ccgctggcgg atgtttctat caccagggca ctgccgccgg aagcgcagca gatgatgcaa
    13861 ttaagcattg agaatggcta taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg
    13921 acgccggagc aaattgataa aatcgcccag ggccacgtct ggaccggtca ggatgcaaaa
    13981 gctaacgggc tggtcgatag tctcggggat ttcgatgatg cggttgccaa agcagcagag
    14041 ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg atgaaccgac cttcttcgac
    14101 aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt tgccagatgc gttccaggcc
    14161 atgttacctg caccgcttgc ctcggtagcc tctaccgtta aaagtgaaag cgacaagctg
    14221 gccgcgttta acgacccaca aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt
    14281 taagtcttgt actgagtggc cgacagatcg tcggccacat tattttttac ctcgacgaat
    14341 cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat agtatcaggt gcgctcctcc
    14401 tgcctcacgt atatactttt gctctttcga tatcattcat atcaatatca tgcaaaagaa
    14461 atcaatttac gtcgcctaca cgggcgggac catcgggatg cagcgttccg agcagggtta
    14521 tattccggtg tcaggtcatc tacaacgcca actggcgctg atgccggaat tccatcgccc
    14581 ggagatgcca gatttcacca ttcatgaata tacgccgttg atggattctt ctgatatgac
    14641 gccggaagac tggcagcata ttgctgaaga tattaaagcg cactatgacg actatgatgg
    14701 ttttgtcatt ctacacggta ccgacacgat ggcgtatacc gcctctgcgc tgtcgttcat
    14761 gctcgagaat ctcggtaaac cggtcattgt gacagggtca caaatcccgc tggctgagtt
    14821 acgctctgac ggacaaatta atctgctgaa tgcgttgtac gttgcggcga attatccgat
    14881 caacgaagta acgctctttt tcaataaccg attgtatcgc ggcaaccgca ctaccaaagc
    14941 ccatgccgat ggttttgatg cgtttgcctc tccaaacctt cctccgttac tggaagctgg
    15001 tatccatatt cgtcgtttga atacgccacc cgccccgcac ggtgaagggg aattaatcgt
    15061 tcatccaatc acgccacaac caattggcgt ggtgacgatt tatccaggga tttctgctga
    15121 cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg attctgcgct cctatggcgt
    15181 gggtaatgcg ccacaaaaca aagccttcct gcaggaatta caagaagcca gcgatcgcgg
    15241 tattgtggtg gtcaacctga cacaatgtat gtccggtaaa gtgaatatga gtggttatgc
    15301 caccggtaac gccctcgccc atgccggcgt tattgacggt gcagatatga ctgtagaagc
    15361 cacgctaacc aaactgcatt acctgctgag ccaggaactg gatactgaaa ccattcgcaa
    15421 ggccatgagc caaaacctgc gcggcgaact gacgccggat gattaaggag actgcaatgc
    15481 cccatcgcgc cctgttactg gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg
    15541 ccgtaccgga aggtgacagt acggtggagg ttgctaaccg ccttattgac tggtgccagt
    15601 cgcgcggaga agcggttatc gccagccagg actggcatcc ggcgaatcac ggcagctttg
    15661 ccagccagca cggcgtagag ccttatacgc caggccaact cggcggcctg cggcaaactt
    15721 tctggcctga acactgtgtg cagaacagtg aaggcgcaca attacatccg ttactgaatc
    15781 aaaaagagat cgcagcggtg ttccataaag gcgaaaatcc tttagttgac agttacagtg
    15841 ccttttttga taacggacgt cggcagaaaa ccgcgcttga tgactggttg cgcgatcatg
    15901 aaatcgatga attgatcgtt atgggcctgg ctactgacta ttgcgtgaag tttaccgtgc
    15961 tggacgcgtt acagttaggt tataaggtaa acgtgattac cgatggctgt cgtggcgtga
    16021 atatccagcc ccaggacagt gcacacgcgt ttatggagat gtcagcagct ggggcaacgc
    16081 tatatacgct ggcagactgg gaagagacgc aggggtaatt ttacgctggc ctacaattct
    16141 gtactggcat tgtaggccaa ataaaacacg tcagtggtac atctggcaat tgatgccatc
    16201 aacgaaagat ttaattcacc tcagaaatct cttccagcga caccttccgc gtttcgatgc
    16261 caaaaatgga gagaaccagc gcacagagca ctaacatgac accaagcacc ataaacaccg
    16321 tgatcgaccc ataatgtgtt aataatgccg caacgccata gggcgtgaaa actgcgacga
    16381 tccgtccgac ggcattaacg aaacccgaac cgcgcaggcg taaatgcgtt ggccaaagct
    16441 ccgggatata aaccgccgac gcgaagcaaa catacatgta taaaaagaag atcatcacca
    16501 gaccatagat taaaatcgcc cactctgtag tctgacttga atagatatag cctaacacgg
    16561 caataataat aagtaaggcg gagccaaata atcgacgagg aaaatgatca ataattaatg
    16621 ccgcaataaa tattcctacc ggagcaccaa tcataataac agcggtcatt aatattgatt
    16681 tatcgacatc aatgccggag ttaacaaata tggtcggtat ccatacggta atggtataaa
    16741 gcgaaatgtt cattgcaatt aaaacagtt
//
LOCUS       DAECOD010000084        16389 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.084, whole genome shotgun sequence.
ACCESSION   DAECOD010000084 DAECOD010000000
VERSION     DAECOD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.084"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="KKO15_002860"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="KKO15_002860"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBD6361578.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="KKO15_002861"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="KKO15_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBD6361579.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="KKO15_002862"
     CDS             2339..2554
                     /locus_tag="KKO15_002862"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361580.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="KKO15_002863"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="KKO15_002863"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBD6361581.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="KKO15_002864"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="KKO15_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBD6361582.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="KKO15_002865"
     CDS             5920..6417
                     /locus_tag="KKO15_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBD6361583.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="KKO15_002866"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="KKO15_002866"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBD6361584.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRARRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="KKO15_002867"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="KKO15_002867"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBD6361585.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="KKO15_002868"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="KKO15_002868"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBD6361586.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="KKO15_002869"
     CDS             9102..9665
                     /locus_tag="KKO15_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBD6361587.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="KKO15_002870"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="KKO15_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBD6361588.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="KKO15_002871"
     CDS             complement(10257..11237)
                     /locus_tag="KKO15_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBD6361589.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="KKO15_002872"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="KKO15_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBD6361590.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRLAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="KKO15_002873"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="KKO15_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBD6361591.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="KKO15_002874"
     CDS             12540..12830
                     /locus_tag="KKO15_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBD6361592.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="KKO15_002875"
     CDS             12838..13431
                     /locus_tag="KKO15_002875"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBD6361593.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="KKO15_002876"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="KKO15_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBD6361594.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="KKO15_002877"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="KKO15_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="KKO15_002878"
     CDS             15882..16385
                     /locus_tag="KKO15_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBD6361595.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4060 a         4218 c         4127 g         3984 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagacgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccacctgc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcctggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgggct
    12061 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtac gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccgcgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAECOD010000085        15931 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.085, whole genome shotgun sequence.
ACCESSION   DAECOD010000085 DAECOD010000000
VERSION     DAECOD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.085"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="KKO15_002879"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="KKO15_002879"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD6361596.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="KKO15_002880"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="KKO15_002880"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBD6361597.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="KKO15_002881"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="KKO15_002881"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBD6361598.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="KKO15_002882"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="KKO15_002882"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBD6361599.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="KKO15_002883"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="KKO15_002883"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBD6361600.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="KKO15_002884"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="KKO15_002884"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBD6361601.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="KKO15_002885"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="KKO15_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="KKO15_002886"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="KKO15_002886"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBD6361602.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="KKO15_002887"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="KKO15_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBD6361603.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..10212)
                     /locus_tag="KKO15_002888"
     CDS             complement(8974..10212)
                     /locus_tag="KKO15_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBD6361604.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGVTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10433..10861
                     /gene="uspG"
                     /locus_tag="KKO15_002889"
     CDS             10433..10861
                     /gene="uspG"
                     /locus_tag="KKO15_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBD6361605.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="KKO15_002890"
     CDS             complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="KKO15_002890"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBD6361606.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="KKO15_002891"
     CDS             complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="KKO15_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBD6361607.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13727..14473
                     /gene="dsbG"
                     /locus_tag="KKO15_002892"
     CDS             13727..14473
                     /gene="dsbG"
                     /locus_tag="KKO15_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBD6361608.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14683..15585
                     /locus_tag="KKO15_002893"
     CDS             14683..15585
                     /locus_tag="KKO15_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6361609.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEYPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15732..15931
                     /locus_tag="KKO15_002894"
                     /pseudo
     CDS             15732..15931
                     /locus_tag="KKO15_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3952 a         4118 c         3854 g         4007 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaccccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg
     9961 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt caacgacttc
    10021 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
    10081 aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa tatcatctgc
    10141 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
    10201 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10261 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt gttcagtgag
    10321 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10381 atattataat catggagaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10441 ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10501 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10561 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10621 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10681 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10741 actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga cccatctgtt
    10801 aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg tcgttcgcta
    10861 acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt tttaatgcca
    10921 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10981 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    11041 cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca
    11101 cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg cggttacgtt
    11161 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca aaaataccgg
    11221 ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga ccaacgactt
    11281 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11341 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattccagca
    11401 gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc
    11461 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11521 tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac
    11581 ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt
    11641 gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat
    11701 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc ccttcagtct
    11761 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11821 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11881 ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11941 cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    12001 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    12061 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc
    12121 tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc gagtaatagg
    12181 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12241 tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca
    12301 gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc aggaaagacg
    12361 gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12421 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta
    12481 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12541 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12601 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12661 aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga atccccggga
    12721 gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat ggcgtgaaag
    12781 acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    12841 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg agctgctttg
    12901 attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    12961 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    13021 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    13081 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    13141 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    13201 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    13261 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    13321 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    13381 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    13441 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    13501 aaaagtaaaa ttgccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    13561 cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    13621 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    13681 ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt taaaaaagat
    13741 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt ccccagtaaa
    13801 agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg gaggaatgaa
    13861 aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc ccgatggtaa
    13921 gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta acacacttat
    13981 cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg aacaatccca
    14041 ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct ccgatccgtt
    14101 ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt
    14161 gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga cagcagcggc
    14221 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggttgcaa
    14281 gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagtgacaa
    14341 tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt acatgagtaa
    14401 ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta atatcattat
    14461 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaaccatc
    14521 ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta gaaaaactta
    14581 atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa acattgatta
    14641 agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa tctctacgac
    14701 ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
    14761 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
    14821 cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg tatcgcgccg
    14881 acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa
    14941 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta cggccctcag
    15001 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga tgcctctgtc
    15061 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
    15121 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
    15181 ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg aattactgac
    15241 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc caaatcagca
    15301 ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa aatttcattt
    15361 cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt actgggaata
    15421 gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga gataaaacta
    15481 gaatatccgt taccttcaat aaagctttac atttcctata ataaatcttc tttaaataat
    15541 ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat aactatagac
    15601 agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa cactctgctt
    15661 tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta acgttcagag
    15721 agtgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg gaagctgcac
    15781 gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg
    15841 gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa atgggcaaaa
    15901 aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DAECOD010000086        15578 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.086, whole genome shotgun sequence.
ACCESSION   DAECOD010000086 DAECOD010000000
VERSION     DAECOD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.086"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..68
                     /locus_tag="KKO15_002895"
     CDS             <1..68
                     /locus_tag="KKO15_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361610.1"
                     /translation="RFAIREGGRTVGAGVVAKVLG"
     gene            251..445
                     /gene="bfd"
                     /locus_tag="KKO15_002896"
     CDS             251..445
                     /gene="bfd"
                     /locus_tag="KKO15_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBD6361611.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            518..994
                     /gene="bfr"
                     /locus_tag="KKO15_002897"
     CDS             518..994
                     /gene="bfr"
                     /locus_tag="KKO15_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBD6361612.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(991..1458)
                     /locus_tag="KKO15_002898"
     CDS             complement(991..1458)
                     /locus_tag="KKO15_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD6361613.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1839..2150
                     /gene="rpsJ"
                     /locus_tag="KKO15_002899"
     CDS             1839..2150
                     /gene="rpsJ"
                     /locus_tag="KKO15_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBD6361614.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2183..2812
                     /gene="rplC"
                     /locus_tag="KKO15_002900"
     CDS             2183..2812
                     /gene="rplC"
                     /locus_tag="KKO15_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBD6361615.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2823..3428
                     /gene="rplD"
                     /locus_tag="KKO15_002901"
     CDS             2823..3428
                     /gene="rplD"
                     /locus_tag="KKO15_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBD6361616.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3425..3727
                     /gene="rplW"
                     /locus_tag="KKO15_002902"
     CDS             3425..3727
                     /gene="rplW"
                     /locus_tag="KKO15_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBD6361617.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3745..4566
                     /gene="rplB"
                     /locus_tag="KKO15_002903"
     CDS             3745..4566
                     /gene="rplB"
                     /locus_tag="KKO15_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBD6361618.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4583..4861
                     /gene="rpsS"
                     /locus_tag="KKO15_002904"
     CDS             4583..4861
                     /gene="rpsS"
                     /locus_tag="KKO15_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBD6361619.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4876..5208
                     /gene="rplV"
                     /locus_tag="KKO15_002905"
     CDS             4876..5208
                     /gene="rplV"
                     /locus_tag="KKO15_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBD6361620.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5226..5927
                     /gene="rpsC"
                     /locus_tag="KKO15_002906"
     CDS             5226..5927
                     /gene="rpsC"
                     /locus_tag="KKO15_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBD6361621.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5940..6350
                     /gene="rplP"
                     /locus_tag="KKO15_002907"
     CDS             5940..6350
                     /gene="rplP"
                     /locus_tag="KKO15_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBD6361622.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6350..6541
                     /gene="rpmC"
                     /locus_tag="KKO15_002908"
     CDS             6350..6541
                     /gene="rpmC"
                     /locus_tag="KKO15_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBD6361623.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6541..6795
                     /gene="rpsQ"
                     /locus_tag="KKO15_002909"
     CDS             6541..6795
                     /gene="rpsQ"
                     /locus_tag="KKO15_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBD6361624.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6960..7331
                     /gene="rplN"
                     /locus_tag="KKO15_002910"
     CDS             6960..7331
                     /gene="rplN"
                     /locus_tag="KKO15_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBD6361625.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7342..7656
                     /gene="rplX"
                     /locus_tag="KKO15_002911"
     CDS             7342..7656
                     /gene="rplX"
                     /locus_tag="KKO15_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBD6361626.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7671..8210
                     /gene="rplE"
                     /locus_tag="KKO15_002912"
     CDS             7671..8210
                     /gene="rplE"
                     /locus_tag="KKO15_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBD6361627.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDSRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8225..8530
                     /gene="rpsN"
                     /locus_tag="KKO15_002913"
     CDS             8225..8530
                     /gene="rpsN"
                     /locus_tag="KKO15_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBD6361628.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8564..8956
                     /gene="rpsH"
                     /locus_tag="KKO15_002914"
     CDS             8564..8956
                     /gene="rpsH"
                     /locus_tag="KKO15_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBD6361629.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8969..9502
                     /gene="rplF"
                     /locus_tag="KKO15_002915"
     CDS             8969..9502
                     /gene="rplF"
                     /locus_tag="KKO15_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBD6361630.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9512..9865
                     /gene="rplR"
                     /locus_tag="KKO15_002916"
     CDS             9512..9865
                     /gene="rplR"
                     /locus_tag="KKO15_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBD6361631.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9880..10383
                     /gene="rpsE"
                     /locus_tag="KKO15_002917"
     CDS             9880..10383
                     /gene="rpsE"
                     /locus_tag="KKO15_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBD6361632.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10387..10566
                     /gene="rpmD"
                     /locus_tag="KKO15_002918"
     CDS             10387..10566
                     /gene="rpmD"
                     /locus_tag="KKO15_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBD6361633.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10570..11004
                     /gene="rplO"
                     /locus_tag="KKO15_002919"
     CDS             10570..11004
                     /gene="rplO"
                     /locus_tag="KKO15_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBD6361634.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11012..12343
                     /gene="secY"
                     /locus_tag="KKO15_002920"
     CDS             11012..12343
                     /gene="secY"
                     /locus_tag="KKO15_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBD6361635.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12375..12491
                     /gene="rpmJ"
                     /locus_tag="KKO15_002921"
     CDS             12375..12491
                     /gene="rpmJ"
                     /locus_tag="KKO15_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBD6361636.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12638..12994
                     /gene="rpsM"
                     /locus_tag="KKO15_002922"
     CDS             12638..12994
                     /gene="rpsM"
                     /locus_tag="KKO15_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBD6361637.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13011..13400
                     /gene="rpsK"
                     /locus_tag="KKO15_002923"
     CDS             13011..13400
                     /gene="rpsK"
                     /locus_tag="KKO15_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBD6361638.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13434..14054
                     /gene="rpsD"
                     /locus_tag="KKO15_002924"
     CDS             13434..14054
                     /gene="rpsD"
                     /locus_tag="KKO15_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBD6361639.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14080..15069
                     /gene="rpoA"
                     /locus_tag="KKO15_002925"
     CDS             14080..15069
                     /gene="rpoA"
                     /locus_tag="KKO15_002925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBD6361640.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPADDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15110..15493
                     /gene="rplQ"
                     /locus_tag="KKO15_002926"
     CDS             15110..15493
                     /gene="rplQ"
                     /locus_tag="KKO15_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBD6361641.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4013 a         3658 c         4148 g         3759 t
ORIGIN      
        1 tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc
       61 tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg ccttttttgc
      121 tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac tatggcgcaa
      181 ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca gaatttgaac
      241 gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc
      301 agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc ctgtgggaaa
      361 tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat
      421 gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc tgctagcccc
      481 atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg ttaaaatcat
      541 aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt attttctcca
      601 tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat accatgaatc
      661 catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt tagaaggaat
      721 ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg agatgctgca
      781 atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc
      841 cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg ctgatgaaga
      901 aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa
      961 ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac tatcccagcc
     1021 tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc
     1081 ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg cagcaaggaa
     1141 aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga gaaacttcac
     1201 atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt aaatgacagc
     1261 gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat caggatgaca
     1321 aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt caggtaacaa
     1381 gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca aaattagaaa
     1441 gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc gaataactcc
     1501 ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt gaaaaacgct
     1561 atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt tgcgattaat
     1621 ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct tgtcgtagtt
     1681 gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca atgctcccaa
     1741 tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt ctgaggagta
     1801 atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa agaatccgta
     1861 tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa atcgtcgaga
     1921 ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca cgcaaagagc
     1981 gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag tacgaaatcc
     2041 gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt gatgctctga
     2101 tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa tcaggtcatt
     2161 gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg gtatgacccg
     2221 tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg aagcaaaccg
     2281 cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg tgaccaccgg
     2341 tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta aagctggcgt
     2401 agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt tcactgtagg
     2461 tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa ctggcacctc
     2521 taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc aggacgctac
     2581 tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc agactccggg
     2641 caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg taaccgttca
     2701 gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta aaggtgctgt
     2761 cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt aaggagatag
     2821 caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa actaccttcg
     2881 gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca gctggtgctc
     2941 gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt aaaaaaccgt
     3001 ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg atctggcgtt
     3061 ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt aacaagaaga
     3121 tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat cgtctgatcg
     3181 ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag aaactgaaag
     3241 acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac ctgttcctgg
     3301 ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac ccggttagcc
     3361 tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt gaggagatgc
     3421 tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg tttctgaaaa
     3481 agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcta aagacgcgac
     3541 caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg aagtcgttaa
     3601 caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc gtcgtagcga
     3661 ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg ttggcggcgc
     3721 tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac atctccgggt
     3781 cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc ttttgctccg
     3841 ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat caccactcgt
     3901 catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg caacaaagac
     3961 ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc gaacatcgcg
     4021 ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg cctgaaagct
     4081 ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac cctgccgatg
     4141 cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg taaaggcggt
     4201 cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg tgcttatgtc
     4261 accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg tgcaactctg
     4321 ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg tgctgcacgc
     4381 tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga ccacccacat
     4441 ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg cgttcagacc
     4501 aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg tcgccgtagc
     4561 aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt ttattgacct
     4621 gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc ccctgcgcac
     4681 ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg ctgtccataa
     4741 tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca aactgggtga
     4801 attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga agaagaaata
     4861 aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc ttctgctcag
     4921 aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc tctggatatt
     4981 ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga atctgccatt
     5041 gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac gaaaattttc
     5101 gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg tgcagatcgc
     5161 atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga ctctggagac
     5221 tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta aaaccatgga
     5281 actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc gattttaaag
     5341 tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc gttatcgagc
     5401 gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc gttatcggta
     5461 aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct ggcgttcctg
     5521 cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg gttgctgaca
     5581 gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag cgtgctgtac
     5641 agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc cgtctgggcg
     5701 gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg cacactctgc
     5761 gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta atcggcgtta
     5821 aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa caaccggaaa
     5881 aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga gcgtcgctga
     5941 tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac cgcggtctgg
     6001 cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc cgtggtcgtc
     6061 tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt aagcgtcaag
     6121 gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg ctggcagtgc
     6181 gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag ccgggtaaag
     6241 tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc aagctggcag
     6301 cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa tgaaagcaaa
     6361 agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc tgctgcgtga
     6421 gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc acctgttgaa
     6481 gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg cgggtgcgta
     6541 atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat ggagaaatcc
     6601 attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt catcaagcgt
     6661 acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga cgtggttgaa
     6721 atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg cgttgtagag
     6781 aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca gaaatgagcc
     6841 gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc tcgatatggg
     6901 gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg agcactaaaa
     6961 tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt cgcgtaatgt
     7021 gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac atcatcaaga
     7081 tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg ctgaaggcgg
     7141 tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt cgcttcgatg
     7201 gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg cgtatttttg
     7261 ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct ctggcaccag
     7321 aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga agttatcgtg
     7381 ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc ttccggcaag
     7441 gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc ggccctgaac
     7501 caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt agcaatcttc
     7561 aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg caaaaaagtc
     7621 cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg atggcgaaac
     7681 tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt aactacaatt
     7741 ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt ggtgaagcga
     7801 tcgctgacaa aaaactgctg gataacgcag cagcagacct agcagcaatc tccggtcaaa
     7861 aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt cagggctatc
     7921 cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt gagcgcctga
     7981 tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag tctttcgaca
     8041 gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa atcgactacg
     8101 ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg aaatctgacg
     8161 aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa ggtagggtta
     8221 ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt tagctgataa
     8281 atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg cttccgacga
     8341 agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca gcccgtctcg
     8401 tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga agttcgggtt
     8461 gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc tgaaaaaggc
     8521 tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca tgcaagatcc
     8581 gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag ctgcggtcac
     8641 catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag aaggttttat
     8701 tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc tgaagtattt
     8761 ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc tgcgcatcta
     8821 taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag ttgtttctac
     8881 ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg gcgaaattat
     8941 ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac cggtcgttgt
     9001 tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag gtaaaaacgg
     9061 cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata ataccctgac
     9121 cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg cgcgtgccct
     9181 gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc agctggttgg
     9241 tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg gtttctctca
     9301 tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc agactgaaat
     9361 cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc tgcgcgccta
     9421 ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag tcgtgcgtac
     9481 caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc gtatccgtcg
     9541 tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg tggtacatcg
     9601 taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag ttctggtagc
     9661 tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta acaaagacgc
     9721 ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca tcaaagatgt
     9781 atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg cagatgctgc
     9841 ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat cgaaaaacaa
     9901 gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac cgttaaaggt
     9961 ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg tcgcgttggt
    10021 tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat ggaaaaagcc
    10081 cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc tgttaaaggt
    10141 gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg tatcatcgcc
    10201 ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct ggctaaagca
    10261 tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct ggaaaatatg
    10321 aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat tctggggaaa
    10381 taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg tcgtctgccg
    10441 aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac cgtagagcgc
    10501 gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt taaagttgag
    10561 gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa ggcgggtaaa
    10621 cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg tcacaaaggt
    10681 cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca gatgcctctg
    10741 taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac agccgaagtt
    10801 cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac gctgaaagcg
    10861 gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg cgaagtaacg
    10921 actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc tatcgaagct
    10981 gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat tagattttca
    11041 aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta tcggtgcgct
    11101 gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg ctgtacttgc
    11161 caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt tctctggtgg
    11221 tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt cggcgtcgat
    11281 cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga aagaagggga
    11341 gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc tggcaatatt
    11401 ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag gcctggtgat
    11461 taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag gaacgatgtt
    11521 cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta tttcaatcat
    11581 tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta tcgagcaagc
    11641 gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag tatttgcagt
    11701 gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa actacgcgaa
    11761 acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc tgaaagtgaa
    11821 tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc cggcgaccat
    11881 cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt cgctgtattt
    11941 gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct tcttctgttt
    12001 cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga agaagtccgg
    12061 tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg ataaagtaat
    12121 gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga tcccggagtt
    12181 catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc ttatcgttgt
    12241 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
    12301 gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg cccgagaagt
    12361 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
    12421 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
    12481 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
    12541 ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc
    12601 agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
    12661 cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg tcggcaagac
    12721 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga tcagtgagct
    12781 gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg ttgaaggtga
    12841 tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt gctatcgcgg
    12901 tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca acgcacgtac
    12961 ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata atggcaaagg
    13021 caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg gctcatatcc
    13081 atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac gcgttgggtt
    13141 gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg tttgcagctc
    13201 aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag aatctggaag
    13261 ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg aacgccgcag
    13321 gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt tgtcgtccgc
    13381 cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag aaaatggcaa
    13441 gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta ttccttaagt
    13501 ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc cagcacggtg
    13561 cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa aaagttcgcc
    13621 gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca gcacgtctga
    13681 aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac aacgttgtat
    13741 accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc cataaagcaa
    13801 ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg aatgacgttg
    13861 taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg gagctggctg
    13921 agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa ggtacgttta
    13981 agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg atcgtcgagc
    14041 tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc tgtgacagag
    14101 tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc caaggtgacc
    14161 cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg ccgtattctg
    14221 ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt actacatgag
    14281 tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg
    14341 ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa atctggcatt
    14401 ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt caagccgcag
    14461 cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat caaagttcag
    14521 cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga tgagcgccca
    14581 atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc ctacaatgtt
    14641 gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga aatggaaacc
    14701 aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa
    14761 ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga agagaaacca
    14821 gagttcgatc cgatcctgct gcgccctgct gacgatctgg aattgactgt ccgctctgct
    14881 aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg taccgaggtt
    14941 gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa agacgtgctg
    15001 gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc aagcatcgct
    15061 gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca tgcgccatcg
    15121 taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt tccgcaatat
    15181 ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag cgaaagagct
    15241 gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg ctaatcgtcg
    15301 tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta acgaactggg
    15361 cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg gcttccgtgc
    15421 aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga aagcagaagc
    15481 tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt ttttataccc
    15541 gtagtatccc cacttatcta caatagctgt actctttt
//
LOCUS       DAECOD010000087        15411 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.087, whole genome shotgun sequence.
ACCESSION   DAECOD010000087 DAECOD010000000
VERSION     DAECOD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.087"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1176)
                     /locus_tag="KKO15_002927"
     CDS             complement(<1..1176)
                     /locus_tag="KKO15_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD6361642.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="KKO15_002928"
     tRNA            complement(1375..1459)
                     /locus_tag="KKO15_002928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="KKO15_002929"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="KKO15_002929"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBD6361643.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="KKO15_002930"
     CDS             2803..4305
                     /locus_tag="KKO15_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBD6361644.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="KKO15_002931"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="KKO15_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD6361645.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="KKO15_002932"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="KKO15_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD6361646.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="KKO15_002933"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="KKO15_002933"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBD6361647.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="KKO15_002934"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="KKO15_002934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBD6361648.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="KKO15_002935"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="KKO15_002935"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBD6361649.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..13066)
                     /locus_tag="KKO15_002936"
     CDS             complement(11870..13066)
                     /locus_tag="KKO15_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBD6361650.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13259..13762
                     /locus_tag="KKO15_002937"
     CDS             13259..13762
                     /locus_tag="KKO15_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6361651.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="KKO15_002938"
     CDS             complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="KKO15_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBD6361652.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14386..15399
                     /gene="argF"
                     /locus_tag="KKO15_002939"
     CDS             14386..15399
                     /gene="argF"
                     /locus_tag="KKO15_002939"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBD6361653.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3936 c         3964 g         3535 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ctcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctatttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgccagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca cccccccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca caccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgt ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcaccccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ctgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttatcg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gccctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa
    12841 caaaaacatt cccaccggta attccataag aaaatcgttg tccgttaact tccatattag
    12901 cataaagata acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa
    12961 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
    13021 caaaagaatg ggacggaaca tccatttcat taataacttg agccataaaa atcccctgaa
    13081 ttaaaaaatc cattgatata atttatagat acagacagta aaatgttctg ccacgttaca
    13141 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagacg cttgcgcacg
    13201 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
    13261 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgcagg ataatcctgc
    13321 tatcgccagc gtcatccgtc aggtttacgc agagtatggt cttaccgccg ataaaggcta
    13381 caccgttgcc gatccgaatc tcgacgaact gtatcaagtg tacagccagc ctggccatgc
    13441 ttattgggtg gttgaataca atggcgaagt ggtcggcggc ggcgggattg cgccattaac
    13501 aggtagtgaa gcggacattt gcgaactgca gaaaatgtac tttcttcccg ctattcgcgg
    13561 taaagggcta gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt
    13621 caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca
    13681 tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg attgtgaagt
    13741 gcggatgcta cgcgaactct aaaaaaatgc ccgctagtat gtgctatcgg gcattttaat
    13801 caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat
    13861 cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct
    13921 ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact
    13981 cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat
    14041 cggtcacttc gtaaccgagt ttaaacgctt caactgccgc tttttccaga gtttccagat
    14101 cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca
    14161 gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg
    14221 ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt
    14281 gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa taatacacat
    14341 aaattgaatt ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat
    14401 cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag
    14461 ttagccgcga agctgaaagc tgataagaaa agcggtaaag aagaagccaa actcactggt
    14521 aaaaacatcg cgctcatctt cgaaaaagac tcgactcgta cccgatgctc tttcgaagtt
    14581 gccgcatatg accagggggc tcgcgttact tatcttggcc caagcggtag tcagattggt
    14641 cataaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga cggtattcag
    14701 tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta
    14761 tggaacggcc tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg
    14821 caggagcatt tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca
    14881 cgtaacaaca tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt
    14941 ctggtcgcgc cacaggcatg ctggccagaa gctgcgctgg ttacggaatg ccgcgccctg
    15001 gcacaacaaa atggcgggaa tattacgctg acggaagatg tagctaaagg agttgaaggt
    15061 gctgacttta tctataccga tgtatgggtg tcgatgggtg aagcaaaaga aaaatgggct
    15121 gaacgcattg cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt
    15181 aacccggagg tcaaattcct ccattgcctg cccgcgtttc atgacgacca aacgacgctt
    15241 ggcaaaaaaa tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc
    15301 ttcgaatctg ccgccagcat tgtatttgat caggcggaaa accgcatgca taccatcaaa
    15361 gcggtgatgg tcgcgacgct cagtaaatta aataattaat atctctataa a
//
LOCUS       DAECOD010000088        15126 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.088, whole genome shotgun sequence.
ACCESSION   DAECOD010000088 DAECOD010000000
VERSION     DAECOD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.088"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(97..1485)
                     /gene="yeaG"
                     /locus_tag="KKO15_002940"
                     /pseudo
     CDS             complement(97..1485)
                     /gene="yeaG"
                     /locus_tag="KKO15_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1921..2667
                     /gene="mipA"
                     /locus_tag="KKO15_002941"
     CDS             1921..2667
                     /gene="mipA"
                     /locus_tag="KKO15_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBD6361654.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2757..3518
                     /locus_tag="KKO15_002942"
     CDS             2757..3518
                     /locus_tag="KKO15_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBD6361655.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3569..4453)
                     /locus_tag="KKO15_002943"
     CDS             complement(3569..4453)
                     /locus_tag="KKO15_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBD6361656.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4537..5532)
                     /gene="gapA"
                     /locus_tag="KKO15_002944"
     CDS             complement(4537..5532)
                     /gene="gapA"
                     /locus_tag="KKO15_002944"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD6361657.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5874..6287
                     /gene="msrB"
                     /locus_tag="KKO15_002945"
     CDS             5874..6287
                     /gene="msrB"
                     /locus_tag="KKO15_002945"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBD6361658.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6329..6601
                     /locus_tag="KKO15_002946"
     CDS             6329..6601
                     /locus_tag="KKO15_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBD6361659.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6971..8047
                     /locus_tag="KKO15_002947"
     CDS             6971..8047
                     /locus_tag="KKO15_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6361660.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            8074..9453
                     /locus_tag="KKO15_002948"
     CDS             8074..9453
                     /locus_tag="KKO15_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361661.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSTNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9470..10515
                     /locus_tag="KKO15_002949"
                     /pseudo
     CDS             9470..10515
                     /locus_tag="KKO15_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10536..11372
                     /locus_tag="KKO15_002950"
     CDS             10536..11372
                     /locus_tag="KKO15_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBD6361662.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11377..12324
                     /locus_tag="KKO15_002951"
     CDS             11377..12324
                     /locus_tag="KKO15_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBD6361663.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12334..13314
                     /gene="ydjG"
                     /locus_tag="KKO15_002952"
     CDS             12334..13314
                     /gene="ydjG"
                     /locus_tag="KKO15_002952"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBD6361664.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13451..14209
                     /locus_tag="KKO15_002953"
     CDS             13451..14209
                     /locus_tag="KKO15_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6361665.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14327..>15126
                     /locus_tag="KKO15_002954"
     CDS             14327..>15126
                     /locus_tag="KKO15_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361666.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVA"
BASE COUNT         3952 a         3330 c         3858 g         3986 t
ORIGIN      
        1 cgtcactctc gtctggggaa tatctccccg gcagccttca gggaaaaata tcatcagatg
       61 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct caatctgctg
      121 ttccaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat cgaagttgaa
      181 cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc cttcatcgac
      241 accggcgtag tcacgatcgt agtcacgata ttcctgatac gacttggctt tcggatcggt
      301 gtctttcaga ctttcgccat cataaacccg catctttgaa taaatgctgg agttttctgg
      361 ctctttcagg cgcgaaagaa tggaaaaacg tgacagtgtt tcgagcgtgc caggggcgca
      421 tggggcgtga gtcaattcac tgtgattaag caatttctcg tagattttga tctcttcgga
      481 aatgcgcaag caatacggca ccttcacgat gtaaacacgg tcgaggaagg cttcgttgtt
      541 tttgttatta cggaaagtga cccattcgga cttgttcgag tgggcgagaa taatcccgtt
      601 gaacggcagg gcggagatac cttccgtccc gttgtagtta ccttcctgag tggcggttaa
      661 caagggatgc agcactttta tcggtgcttt aaacatctca acgaattcca tgatcccctg
      721 attggcgcgg cacagcgcac cggaatagcc gtaggcgtcc gggtcattct gcgcgtagtg
      781 ttcgagttta cgaatatcga ctttcccaac cagcgcggag atgtcctggt tgttctcatc
      841 accgggttcc gttttggcga tagcaatttg ttgcagaatt gacggccaga ccttcactac
      901 ccggaactta gtgatatcgc caccaaattc atgcaggcgt tttgccgccc atggcgacat
      961 gatggtgccg agataacggc gaggaatgcc atactctttt tccagaatct gagcatcttc
     1021 ctgcggattg aaaagacaga acggatgatc gttgaccggg ctacgctcac cgttggcgct
     1081 caatacataa atcggtacga gctgcattaa tgatttcagt cgctcagcaa gcgatgattt
     1141 acccccaccc acaggcccca gcagatacag gatttgtttc ttctcttcca gcccctgagc
     1201 cgcgtgtttc agataagaga caatctgttc aatcgcgtct tccatgccgt aaaactcttc
     1261 aaatgccgga taacgtgcaa tgacccggtt agaaaagagt cgagaaagtc tggggtcctg
     1321 ggctgtatcg accatgacag gctcaccgat agccatcaat agccgctcag ccgcgttggc
     1381 ataagcactg cgatcttgcc gacaagtggt aagaaactcc tgcagtgtga actcttcgtc
     1441 cttggcagct tcatatcgct ggcgatagtg atcgaatata ttcatggtat gccgtccttt
     1501 cgttttttag cacaggttaa gagccgttca tatgaatagt agaggctccc ggaagaggta
     1561 aactgaacga cgtctcttgc taaaacacag caactctcat gccattttat cgacgatgag
     1621 aacaacgtgc ccggtgatac accttcaaaa ttaagcgtag atggcaaatg gaaaacttgc
     1681 ctgcacggct ttcttaattt caatggcata tcaacaactc atccataaat tttgcataat
     1741 taatgtaaag accaggctcg ccagtaacgc taaatccatt tggctgtaag cgcggtgtca
     1801 tccgcggcag gaaaattaaa cagttacttt aaaaaatgaa aacgtaaaaa ggttgggttt
     1861 cgatgtattg acgggtaaac tttgtcgccc gctaaacatt tgtttaagga atgattaatt
     1921 gtgaccaaac tcaaacttct ggcacttgga gtgcttatcg caacgtctgc aggcgtagcg
     1981 cacgctgaag gtaaattttc cctgggcgca ggcgtaggtg tcgttgagca cccatataaa
     2041 gattacgata ccgatgttta cccagtaccg gtaatcaact atgaaggcga taacttctgg
     2101 ttccgtggct taggtggtgg ttactacctg tggaatgacg caacggataa actttcaatt
     2161 accgcttact ggtcgccgct ttacttcaaa gcgaaagaca gtggcgatca ccaaatgcgt
     2221 cacctggatg accgtaagag caccatgatg gctggtctgt cttatgctca ctttacccag
     2281 tacggttacc tgcgtaccac cctggctggc gataccctgg ataacagcaa cggcattgtc
     2341 tgggatatgg cctggttgta tcgttacacc aacggtggcc tgaccgtgac tccgggtatt
     2401 ggtgtgcagt ggaacagcga aaaccagaac gaatactatt atggcgtatc gcgcaaagag
     2461 tccgctcgca gcggtctgcg tggctataac ccgaatgaca gctggagccc ttaccttgag
     2521 ctgagcgcca gctacaactt cctcggcgac tggagtgttt acggtaccgc gcgctacacc
     2581 cgtctgtctg atgaagttac tgacagcccg atggtggata aatcctggac tggcctgatt
     2641 tctaccggga tcacctacaa attctgataa tgcatctttc cagggcattt tgtgcataaa
     2701 aacagggcgt cacgccctgt tttgcattac ggtggtgcaa tacctgggag gaaacaatgc
     2761 aacaaaaaat gattcaattt agtggcgatg tctcactgcc agccataggg cagggaacat
     2821 ggtatatggg cgaagatgcc agtcagcgca aaacagaagt tgctgcacta cgcgcaggca
     2881 ttgaactcgg tttaaccctc attgataccg ccgaaatgta tgccgatggc ggtgccgaaa
     2941 aggtggttgg ggaagcatta accggtctgc gagagaaggt ctttctcgtc tctaaagtct
     3001 atccgtggaa tgctggcggg caaaaagcga taaatgcatg cgaagccagt ttacgccgtc
     3061 tcaatactga ctatctcgat ctctacttat tacactggtc tggcagtttc gcttttgaag
     3121 agactgttgc tgcgatggaa aaattgatcg cccagggaaa aatccgtcgc tggggcgttt
     3181 ctaaccttgg ttatgccgat atgcaggaac tctggcagct gccgggggga aatcagtgtg
     3241 ccactaatca ggtgctttac catctcggtt cacgagggat tgagtacgat ctactcccct
     3301 ggtgccagca acagcagatg ccggtgatgg cttacagtcc attagcccag gccgggcggt
     3361 tgcgcaatgg actgttaaaa aacgcggtag tcaacgaaat tgcacatgtc caacaacatg
     3421 cagctgcgct tgaggtcgaa ctttcgtcag cggaattagc tatgctggat aaggcatatc
     3481 cggcaccaaa aggaaaaact gcgctggata tggtgtgatg ttgatacgtg ccggatgcat
     3541 cgcacccggc acacaacatt aacgcaaatt aacgtttggc aacgcgaatg gattgcgcca
     3601 gatgtgcagg tttctctttg gtgacttttt gcgtttctga agcgtaagcc gtttctacac
     3661 aaacaaaggt cttgtagcca tcgtccggca tgtcgcccat gctaactgaa agcgccggtc
     3721 ccgggttcca gccgacaacg ttcagatgat gctggtggcc tacggcgata atacgattca
     3781 gcgcttcatc attaatcacg ctgcaatctt gcggattcag atacacgcga tcggtacggt
     3841 cagggaaggt ctgaataccg tcggtcagta catcttcttt cgcgtcattc actttatcaa
     3901 tgaagcgatc gcccagccca ctgacgctta ccttagcgat atcacccacg ttaaagtagg
     3961 tatgcagggc agaggtggtt tcaaattcgc catgtgattc aagatcgatt tcacaggttt
     4021 tacccacgcg gaaatgcgct aacagcgtaa agtcgtgcgg ccagaatttt ttcgtctctt
     4081 cgctttgtgt caattcaaaa gtcagcgcta cgccatcagc atcttcatga tgtgatttca
     4141 gcgtccacgg caggttgcgg gcaaaaccgt gcgcaggcag accttgttgt gccgccggac
     4201 caaaccacgg ccagcaaacc ggtacgccac cgcgaatagc gacgccattt ttgaacggtg
     4261 tgttgttgct caaccacaga acttcttctt cacccgcagg tttccacgag agaaggtgtg
     4321 cgccctgtaa tgcaaaagag gcttttacct ggggatgatc gaccacaatg aggtccagtt
     4381 catccagttt acgacgggag aggacagggg agatttgttc gatgaccgga agggcaaaaa
     4441 ttttcttaat catgacgcag tcctttaact tcattttatc aggtaaaaaa aagagcgacc
     4501 gaagtcgctc tttttagatc acagtgtcat ctcaacttat ttggagatgt gagcgatcag
     4561 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
     4621 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
     4681 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
     4741 agcagcagct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
     4801 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
     4861 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
     4921 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
     4981 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
     5041 atcgttgata actttagcca gcggagctag gcagttggtg gtgcaggaag cgttggaaac
     5101 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
     5161 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
     5221 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccca cttcgtccca
     5281 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
     5341 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
     5401 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
     5461 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     5521 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     5581 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     5641 tgcctaagca ttacgcgact gactcgcctc acttttcctt tgggcttgag accacatggg
     5701 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     5761 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     5821 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag taaatggcga
     5881 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     5941 agaatcatgg gacagaaccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     6001 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     6061 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     6121 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     6181 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     6241 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     6301 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     6361 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     6421 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     6481 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     6541 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctatagcca tgtttaagta
     6601 agtggttcat cccctttttg ccgcggctat gaaggggata gccaacaatg accccacgac
     6661 tcgtctcaat tatctcgacc cgacctggct aagtcccagt ccgatctctt gcagtcgcgt
     6721 tcacgcctga atgacttctc ctgaacttaa gtctcagacc tatttggccg gtaatccctc
     6781 tcgaatttgc tctttttgcg tcccgccttc caccaacctg taacagaccc agccattttc
     6841 tgcgtaattg tgatctgagt tgtaaaatgc aggtcattat ctgtgttgtg cagatttaat
     6901 attgacctca aaaatcatgt gggtaagtat gatggccatc taagcaaaac aaaacacaga
     6961 ggatgacgca atgaaagcac tggctcggtt tggcaaggcc tttggcggct acaagatgat
     7021 tcatgtccca caacccattt gtggcccgga agacgtagtg attgaaatta aagccgcggc
     7081 aatctgcggc gcagacatga agcactacaa tgtcgatagc ggttctgatg agtttaactc
     7141 tatccgcggc catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg
     7201 gaaagtgggg caacgcgtcg tgtcggataa cagcggtcac gtttgcggcg tttgtccggc
     7261 ctgtgaacag ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa
     7321 tacctggggc ggtggttttt ccaaatattg tctggttcct ggtgaaattc tcaaaattca
     7381 tcgtcatgcg ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga
     7441 ccctatctgt aatgcctata aatccatcgc gcagcaatcg aaattccttc ctggtcagga
     7501 tgttgtcgtc attggcactg gcccactcgg gctgttttcc gtacaaatgg cgcggattat
     7561 gggggcggta aatatcgtcg tcgttggtct gcaagaagat gtggcggtcc gcttcccggt
     7621 tgcaaaagaa ctgggggcga cggcagtagt aaatggttct accgaagatg tggtggcgcg
     7681 ctgccagcaa atttgtggca aagataatct gggactggtg attgaatgct ccggtgccaa
     7741 tatcgcactg aaacaagcca tcgaaatgct ccgcccgaat ggggaagtgg tacgcgttgg
     7801 aatgggcttc aaacctcttg atttctcgat taatgacatt accgcctgga ataaaagcat
     7861 cattgggcat atggcctatg actccacctc atggcgtaac gctatccggc tattagccag
     7921 cggcgctatc aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga
     7981 agggtttgat gcgatggtcg ataaaaccgc aatcaaagtg atcatgactt acgactttga
     8041 tgaataacta ctctcctgaa ggaacgttat cgcatggaac agataacaaa accgcattgt
     8101 ggtgcacggc tggatcgctt accggattgc cgctggcatt cgtcaatgtt tgccatcgtc
     8161 gcgtttggct tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg
     8221 aaagcgttgg gctggacaga taattccact acagccacat tctcagcaat cacgaccgcc
     8281 ggaatgtttc tcggtgcgct ggtgggcggc atcattggcg acaaaaccgg tcgcagaaat
     8341 gcgttcatcc tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaacg
     8401 aatatggatt ttcttattgc ctgccgtttt gtgatggacg ttgggctggg agctttactg
     8461 gttacactgt ttgctggttt caccgaatat atgcccggta gaaatcgcgg aacgtggtcc
     8521 agtcgggttt cctttattgg caactggtca tatccgctct gctcactgat tgcgatggga
     8581 ctcacgccgc tgattagtgc agagtggaac tggcgggtac aactgcttat tcctgcaata
     8641 ttgtcgctta tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg
     8701 gaatcgcgcg gacggtatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc
     8761 atacgccaga cgggtaaacc tttgccgcct gtggttattg ctgatgacgg taaagcgcca
     8821 caagcggtgc cgtattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc
     8881 tcttgtgtgc tgattaccat gaacgttgtg cagtacacac taattaactg gttgccaaca
     8941 atattcatga cccaggggat taatttaaaa gactcgattg ttttaaatac catgagtatg
     9001 tttggcgcgc catttggtat ttttattgcc atgctggtga tggataaaat tccgcgtaaa
     9061 acaatgggtg tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa
     9121 accagtatgt tgctcataac gctgattggt ttcttcctga ttactttcgt ctatatgtac
     9181 gtttgctatg cctcggcagt gtatgtccct gaaatctggc cgacagaggc caaactccgt
     9241 ggctccggtc tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt
     9301 gcagtgctgc tcagtagtta tggtgtaacg ggagtcttta ttcttctggg ggctgtttca
     9361 aatattgtcg caattgctat cgccaccatt ggaattgaaa ccaaaggtgt ctccgttgaa
     9421 agtttaagta ttgatgcagt agccaataaa taatacgggt ggattcaaaa tgaaaaattc
     9481 aaaagcaata ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa taccagtgcc
     9541 taaagaagat gaagttttga ttaaagtaga atatgtcggt atttgtggtt cagatgtaca
     9601 tggttttgaa tcaggcccgt ttattccgcc taaagaccca aatcaagaaa ttggcctggg
     9661 tcatgaatgc gccgggacgg ttgtggctgt gggaagccgc gtgcgcaaat ttaaaccggg
     9721 ggatcgggta aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg
     9781 caaatataac atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc
     9841 attaacgcac tatctgtgtc atccggagag ctttacttac aaactgcccg acaatatgga
     9901 cacgatggaa ggggcgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc
     9961 agatgttaaa ccgggtaaga agataattat tctgggagca ggttgtattg gtttgatgac
    10021 gttgcaagcg tgcaaatgcc tgggcgcaac ggaaattgcc gtcgttgatg tactggaaaa
    10081 acgtctggca atgggggaac agcctggtgc gacagtggtt attaatggcg caaaagagga
    10141 cactattgca cgctgtcagc aatttaccga agacatgggc gcagatattg ttttcgaaac
    10201 agcgggttct gcggtcaccg ttaaacaggc acctttaatc tggtaatgcg cggcggtaaa
    10261 attatgattg ttggtactgt acccggcgat tcggcaatca atttcctcaa aatcaatcgc
    10321 gaagtcacta tccagacggt attccgctat gccaatcgtt atccggtcac gattgaagct
    10381 atttcttcag ggcgattcga tgtgaaatcg atggtgacgc atatttacga ttatcgggat
    10441 gtacaacagg catttgaaga gtcagttaac aacaaacgcg acattattaa aggcgttatt
    10501 aaaattagcg attaattaat acgggagaac agaacatgct cgcagatatc agatattggg
    10561 aaaacgatgc cacaaataaa tattatgcaa ttgcccattt taacgtatgg aatgcagaaa
    10621 tgttgatggg cgttaaagac gcagccgaag aagcgaaatc cccggttatt atttctttta
    10681 gtacaggttt tgtcggtaac acctcatttg aagatttctc tcacatgatg gtatcaatgg
    10741 cacaaaaagc aacggtgccg gtaataactc attgggatca tggtcggagt attgagatta
    10801 ttcataacgc ctggactcat ggtatgaatt cattaatgcg tgatgcttct gcatttgatt
    10861 tcgaagaaaa tattcgttta accaaagagg ctgttgattt ctttcatccg ctgggtattc
    10921 cggtagaggc ggaattaggg catgtcggta atgaaaccgt ttatgaggaa gcgctggcgg
    10981 gctatcacta taccgatcct gaccaggcgg ctgaatttgt tgaacgtacg ggctgtgact
    11041 cattggctgt cgccatcggc aaccagcatg gggtttatac gtcagagccg caattgaact
    11101 ttgaggtcgt caaacgcgta cgcgatgctg tttctgttcc gctggttttg catggcgcat
    11161 cggggatcag tgatgccgac attaaaactg caatttcgtt gggtattgcg aaaatcaaca
    11221 ttcatacgga gctctgtcag gccgcaatgg tagcagtgaa agaaaaccag gatcagcctt
    11281 tcctgcattt agaacgtgaa gtacgtaaag ctgtaaaaga acgggcattg gaaaaaattg
    11341 aactgttcgg ttctgatggc aaagcggaat aatgatatgg ataatctcga cgttatttgt
    11401 ataggtgccg ctattgttga tattccatta caaccggtca gtaaaaatat ctttgatgta
    11461 gattcttatc ctcttgaaag aatcgcaatg agcaccgggg gcgatgcaat aaatgaagca
    11521 acaattattt ctcgtctggg gcatcgtacc gcattaatga gccgcattgg taaagatgcc
    11581 tcagggcaat ttattctcga ccattgtcgc aaagagaata ttgatattca aagcctgaaa
    11641 caggacgtca gcatagatac atctattaac gttggactgg tgacggagga tggcgagcgg
    11701 acgtttgtca cgaaccgtaa cggcagtttg tggaaattaa atattgacga tgttgatttt
    11761 gcgcggtttc ctcaggcgaa attattatca ctggccagta ttttcaacag tcctctattg
    11821 gatggtaaag cgctaacaga aatttttacg caagcgaaag cccggcagat gatcatttgt
    11881 gccgatatga tcaaaccgcg gttgaatgaa acgctggatg atatttgcga agcattgagt
    11941 tatgtcgatt atctgtttcc aaattttgcc gaggaaaaat tactcacagg gaaagagaca
    12001 ctggatgaaa ttgctgactg ctttcttgcg tgcggcgtaa aaacggtggt gattaaaacg
    12061 ggtaaagacg gctgctttat caagcgtggt gacatgacga tgaaggtgcc ggcggtcgca
    12121 ggaataaccg ccatcgacac cattggcgcg ggcgataact ttgcttcagg ttttattgcg
    12181 gcactgttag aaggcaaaaa tctgcgtgaa tgcgcacgct ttgccaatgc aacggcggct
    12241 atctcggttc taagcgtcgg tgccaccacc ggcgtaaaaa acagaaagct ggtcgaacaa
    12301 ttgctggaag aatacgaagg ataatgaagg caaatgaaaa agataccttt aggcacgacg
    12361 gatataacgc tttcgcgaat ggggttgggg acatgggcca ttggcggcgg tcctgcatgg
    12421 aatggcgatc tcgatcggca aatatgtatt gatacgattc ttgaagccca tcgttgtggc
    12481 attaatctga ttgatactgc gctaggatat aactttggca atagtgaagt tatcgtcggt
    12541 caggcgttaa aaaaactgcc ccgtgaacag gttgtagtag aaaccaaatg cggcattgtc
    12601 tgggaacgaa aaggaagttt attcaacaaa gttggcgatc ggcagttgta taaaaacctt
    12661 tccccggaat ctatccgcga agaggtagaa gccagcttgc aacgtctggg tattgattac
    12721 atcgatatct atatgacgca ctggcagtcg gtgccgccat tttttacgcc gatagctgaa
    12781 actgtcgcag tgcttaatga gttaaaggct gaagggaaaa ttcgcgctat aggcgctgct
    12841 aacgtcgatg ctgaccatat ccgcgagtat ctgcaatatg gtgaactgga tattattcag
    12901 gcgaaataca gtattctcga ccgggcaatg gaaaacgaac tgctgccgct atgtcgtgat
    12961 aatggcattg tggttcaggt ttattccccg ctagagcagg gattgttgac cggcaccatc
    13021 actcgtgatt acgttccggg cggcgctcgg gcaaataaag tctggttcca gcgtgaaaac
    13081 atgctgaaag tgattgatat gcttgaacag tggcagccac tttgtgctcg ttatcagtgc
    13141 acaattccca ctctggcact ggcgtggata ttaaaacaga gtgatttaat ctccattctt
    13201 agtggggcta ctgcaccgga acaggtacgc gaaaatgtcg cggcactgaa tatcaactta
    13261 tcggatgcag acgcaacatt gatgagggaa atggcagagg ccctggagcg ttaaatactc
    13321 aacacgattc acctctgttt ctgtgaaaac tgtgatgaaa atctgtgatt caaataggtt
    13381 attttgaaag taaacatcgg ttatgcgata atcgcgctaa tgtgatgtaa aaagatacag
    13441 gggtgtaatc gtggcggcaa aagacaggat tcaggcaatt aagcaaatgg ttgccaacga
    13501 taaaaaggtg acagtctcaa atttgagtgg gatttttcag gtaaccgaag aaaccattcg
    13561 ccgcgatctt gagaagctgg aagatgaagg ctttttgacc agaacctatg gtggtgctgt
    13621 tttaaataca gcggtgttga cggagaatat ccatttttat aagcgcgctt catcgtttta
    13681 tgaagagaag ctgcttattg cacgcaaggc actacccttt atcgacaata aaaccaccat
    13741 ggcagccgat tccagtagta ccgtaatgga attgctcaaa ttgttacagg accgtagtga
    13801 cctgacgttg ctaaccaact cggcggaagc gattcatgtt ctggctcagt cagaaattaa
    13861 agtcgtttca acgggtgggg aactaaacaa aaatacactt tccctgcaag gaagaattac
    13921 taaagagatc atcagccgct accatgtcga tatcatggta atgagttgta aaggtcttga
    13981 tattaacagc ggcgcgctgg actctaatga agcggaagct gaaatcaaaa agacaatgat
    14041 ccgtcaggcg acagaagttg cgttattggt tgatcactct aagtttgatc gtaaagcttt
    14101 tgtccagtta gctgacttta gtcatattaa ttatataata actgataaat caccgggtgc
    14161 agagtggata gcattttgca aagacaataa tatccagctg gtgtggtaat ttaagcacac
    14221 gttgtcaata ttatttgttc ctgcgactgg aaataaaatg atgataaaga tgcggcaagt
    14281 gaatttgccg caattgctac cttttatagc aaatccgagg cacccaatgg aacaatatga
    14341 tcaaattggc gcaagactgg accgcttgcc tttggcccgg tttcattatc gtatatttgg
    14401 tataataagc tttagtctgt tattaacggg gtttttgagt tactcaggta atgtcgtctt
    14461 agcaaagctg gtaagcaatg gatggtcaaa taatttcctc tatgccgcct ttacctcggc
    14521 attaatgttt ggttatttca tcggctcact tactggtggg tttattggtg actactttgg
    14581 gcggcgcagg gcgtttcgca taaatcttct catcgtcggt attgctgcaa caggggccgc
    14641 ttttgtccct gatatgtact ggctcatttt ctttcgcttc ctgatgggaa caggaatggg
    14701 ggcgctgatt atggttggct atgcctcatt tacggagttt atccccgcga cggtgcgtgg
    14761 aaaatggtcc gcgcggctct catttgttgg taactggtcg cccatgctgt ctgcggcgat
    14821 aggcgtggtg gttatcgctt tttttagttg gcgaataatg tttcttctgg gtggtattgg
    14881 catactgtta gcctggtttc tctcaggtaa atactttatc gagtcgccac gatggctgga
    14941 agggaaaggg caaatcgccg gtgcagaaag ccaacttcgt gaagtagagc agcaaattga
    15001 aagggagaag agtattcgtt taccccagct tactttgaac cagagcaaca gcaaggttaa
    15061 ggtaatcaag ggtaccttca ggctcctgtt taaaggggaa atgttacgac gtacattagt
    15121 tgcgat
//
LOCUS       DAECOD010000089        15058 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.089, whole genome shotgun sequence.
ACCESSION   DAECOD010000089 DAECOD010000000
VERSION     DAECOD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.089"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="KKO15_002955"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="KKO15_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBD6361667.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="KKO15_002956"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="KKO15_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBD6361668.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="KKO15_002957"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="KKO15_002957"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBD6361669.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="KKO15_002958"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="KKO15_002958"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBD6361670.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="KKO15_002959"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="KKO15_002959"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBD6361671.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="KKO15_002960"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="KKO15_002960"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBD6361672.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="KKO15_002961"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="KKO15_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBD6361673.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="KKO15_002962"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="KKO15_002962"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBD6361674.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="KKO15_002963"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="KKO15_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBD6361675.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="KKO15_002964"
     CDS             7139..7750
                     /locus_tag="KKO15_002964"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBD6361676.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="KKO15_002965"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="KKO15_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD6361677.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="KKO15_002966"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="KKO15_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="KKO15_002967"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="KKO15_002967"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBD6361678.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="KKO15_002968"
     CDS             complement(11944..12303)
                     /locus_tag="KKO15_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361679.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRIYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12806)
                     /locus_tag="KKO15_002969"
     CDS             complement(12300..12806)
                     /locus_tag="KKO15_002969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361680.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="KKO15_002970"
     CDS             complement(12952..13515)
                     /locus_tag="KKO15_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361681.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="KKO15_002971"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="KKO15_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..>15058)
                     /locus_tag="KKO15_002972"
     CDS             complement(14723..>15058)
                     /locus_tag="KKO15_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD6361682.1"
                     /translation="SYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSF
                     FANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGY
                     CASKYFIFR"
BASE COUNT         4160 a         3651 c         3475 g         3772 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgagcgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggaccaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgaggtatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagat tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca gtttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataag gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataact
//
LOCUS       DAECOD010000090        15047 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.090, whole genome shotgun sequence.
ACCESSION   DAECOD010000090 DAECOD010000000
VERSION     DAECOD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.090"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..237
                     /locus_tag="KKO15_002973"
     CDS             <1..237
                     /locus_tag="KKO15_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBD6361683.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="KKO15_002974"
     CDS             247..885
                     /locus_tag="KKO15_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBD6361684.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="KKO15_002975"
     CDS             888..2051
                     /locus_tag="KKO15_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD6361685.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3760
                     /locus_tag="KKO15_002976"
     CDS             2135..3760
                     /locus_tag="KKO15_002976"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD6361686.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="KKO15_002977"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="KKO15_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBD6361687.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="KKO15_002978"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="KKO15_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBD6361688.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="KKO15_002979"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="KKO15_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6361689.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="KKO15_002980"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="KKO15_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBD6361690.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="KKO15_002981"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="KKO15_002981"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBD6361691.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="KKO15_002982"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="KKO15_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBD6361692.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="KKO15_002983"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="KKO15_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBD6361693.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="KKO15_002984"
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="KKO15_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBD6361694.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="KKO15_002985"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="KKO15_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD6361695.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11560
                     /gene="ulaE"
                     /locus_tag="KKO15_002986"
     CDS             10706..11560
                     /gene="ulaE"
                     /locus_tag="KKO15_002986"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBD6361696.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11560..12246
                     /gene="ulaF"
                     /locus_tag="KKO15_002987"
     CDS             11560..12246
                     /gene="ulaF"
                     /locus_tag="KKO15_002987"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBD6361697.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12375..12650)
                     /locus_tag="KKO15_002988"
     CDS             complement(12375..12650)
                     /locus_tag="KKO15_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6361698.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12977..13372
                     /gene="rpsF"
                     /locus_tag="KKO15_002989"
     CDS             12977..13372
                     /gene="rpsF"
                     /locus_tag="KKO15_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBD6361699.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13379..13693
                     /gene="priB"
                     /locus_tag="KKO15_002990"
     CDS             13379..13693
                     /gene="priB"
                     /locus_tag="KKO15_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBD6361700.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13698..13925
                     /gene="rpsR"
                     /locus_tag="KKO15_002991"
     CDS             13698..13925
                     /gene="rpsR"
                     /locus_tag="KKO15_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBD6361701.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13967..14416
                     /gene="rplI"
                     /locus_tag="KKO15_002992"
     CDS             13967..14416
                     /gene="rplI"
                     /locus_tag="KKO15_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBD6361702.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14487..>15047)
                     /locus_tag="KKO15_002993"
     CDS             complement(14487..>15047)
                     /locus_tag="KKO15_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD6361703.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3514 a         3667 c         4059 g         3807 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgtatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttgatctccc acagcacgcg
     6601 gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggagt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca acatgtgatt ggttttgatt aatcctgaca ctattccttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt
     7681 tcgttgtgtg aatcacactc tatccgtgtg gaattatttg cgggtcgcgt cacatttaat
     7741 cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     9901 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 agcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactaacgag cgcctgtcgc gcctcgactg
    10861 gagccgcgaa cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgtg attgggtgaa
    11521 agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag
    11581 gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac ttggggcaac
    11641 gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac
    11701 gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg
    11761 gagtatcgcc catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg
    11821 cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg
    11881 gcgatcccgg cattaggcac cacgcacgct gactacttct ttggtgatat cccgtgtacg
    11941 cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt
    12001 atcgaaacgc tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac
    12061 ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa
    12121 gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac
    12181 agctacctga tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag
    12241 aagtaaaaca cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag
    12301 ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt
    12361 ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc
    12421 ggttcccgca tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt
    12481 aatgcctgac gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag
    12541 ctttgcacat cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta
    12601 tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc
    12661 tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat
    12721 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga
    12781 atgtgatgaa cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct
    12841 gctccgccct tgagtttagc ggggtatgcc agtactataa cgcgtcattt ttcagccgac
    12901 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
    12961 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
    13021 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
    13081 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
    13141 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
    13201 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
    13261 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
    13321 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
    13381 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
    13441 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
    13501 ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc
    13561 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
    13621 caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
    13681 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
    13741 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
    13801 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
    13861 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
    13921 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
    13981 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
    14041 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
    14101 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc
    14161 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
    14221 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
    14281 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
    14341 tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
    14401 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg
    14461 ttttgctttc tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct
    14521 tgtgtccata agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct
    14581 cggtcctgcg gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag
    14641 gaacgtagac caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca
    14701 atgatgaatc cgagggcatt agaaacgcct gtaacagtgt gaagttggtc gatataaaga
    14761 tcgattgttg gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg
    14821 tatcctatcg tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg
    14881 tgtattgtgt atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt
    14941 tctgcggtta acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct
    15001 aatgtagctt catggttcca tccaaaagaa ttatcaccat tcattgt
//
LOCUS       DAECOD010000091        14974 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.091, whole genome shotgun sequence.
ACCESSION   DAECOD010000091 DAECOD010000000
VERSION     DAECOD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.091"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..176)
                     /locus_tag="KKO15_002994"
     CDS             complement(<1..176)
                     /locus_tag="KKO15_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361704.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="KKO15_002995"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="KKO15_002995"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBD6361705.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="KKO15_002996"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="KKO15_002996"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBD6361706.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="KKO15_002997"
     CDS             complement(2012..2884)
                     /locus_tag="KKO15_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBD6361707.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="KKO15_002998"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="KKO15_002998"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBD6361708.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="KKO15_002999"
     CDS             complement(3576..4361)
                     /locus_tag="KKO15_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6361709.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="KKO15_003000"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="KKO15_003000"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBD6361710.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5459..6279
                     /locus_tag="KKO15_003001"
                     /pseudo
     CDS             5459..6279
                     /locus_tag="KKO15_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            6303..7181
                     /gene="yihT"
                     /locus_tag="KKO15_003002"
     CDS             6303..7181
                     /gene="yihT"
                     /locus_tag="KKO15_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBD6361711.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7198..8439
                     /gene="yihS"
                     /locus_tag="KKO15_003003"
     CDS             7198..8439
                     /gene="yihS"
                     /locus_tag="KKO15_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBD6361712.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8577..9479
                     /locus_tag="KKO15_003004"
     CDS             8577..9479
                     /locus_tag="KKO15_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBD6361713.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9678..11714
                     /gene="yihQ"
                     /locus_tag="KKO15_003005"
     CDS             9678..11714
                     /gene="yihQ"
                     /locus_tag="KKO15_003005"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBD6361714.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11760..13134
                     /locus_tag="KKO15_003006"
                     /pseudo
     CDS             11760..13134
                     /locus_tag="KKO15_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13177..14580
                     /locus_tag="KKO15_003007"
     CDS             13177..14580
                     /locus_tag="KKO15_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361715.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14648..>14974
                     /gene="ompL"
                     /locus_tag="KKO15_003008"
     CDS             14648..>14974
                     /gene="ompL"
                     /locus_tag="KKO15_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBD6361716.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3677 a         3859 c         3725 g         3713 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatc tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 atcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aatgccatta ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt
     5821 gccacaccga tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg
     5881 gggatccgcg ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca
     5941 gaagccgccg ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg
     6001 agcggtaccg ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc
     6061 ggagatcttt ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt
     6121 gatgtcgcca accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat
     6181 agccaggcgc gcgcagcggg tcacggtcgc caggactggt ccgccattct ggaacaggtc
     6241 cgtgtcagtg ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat
     6301 gaatgaataa gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg
     6361 cggtcgatca gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg
     6421 tagccgatag cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg
     6481 cctcggcgat tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga
     6541 ttgccaaaag ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc
     6601 cggtcgatag cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg
     6661 gcaaagcctt aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg
     6721 atatggtgaa agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc
     6781 cagtcgtccg cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg
     6841 ccgccaaaga gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg
     6901 gcaaaggtcc gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata
     6961 tgccatgggt gatcctctct tccagtgtcg acgaaaaact gttcccgcgt gccgtacgcg
     7021 tggcaatgac ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg
     7081 tcggtttacc agacaacgag ctgatgctcc gtgacgtttg cgcaccgaaa ttacaacaac
     7141 ttggcgatat cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg
     7201 aaatggttta acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt
     7261 gattttggta aaaattccgt agtgccgact ggttttggct ggttaggcaa taaagggcaa
     7321 atcaaagaag agatgggcac ccatctgtgg atcacagctc gtatgttgca cgtttattcc
     7381 gttgctgcgg cgatgggtcg acctggcgct tacgcgttgg ttgatcacgg tattaaagcc
     7441 atgaacggcg cactgcgtga taaaaaatat ggcggctggt atgcctgcgt gaatgacgag
     7501 ggcgtggtgg atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc
     7561 agcgccgtca caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgagatt
     7621 atcgagaaat atttctggag cgaagaagag cagatgtgcc tggagtcctg ggacgaagcc
     7681 ttcagcaaaa ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc
     7741 ttgattgttt atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct
     7801 tccgtgatta tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat
     7861 acccagtgga atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg
     7921 ttcggaggta caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct
     7981 gccctggaag cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg
     8041 tttaacgcca ccgtgcgcga tgcctgggca cccgatggag cggacgggat tgtttatacc
     8101 gttgactggg aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca
     8161 atgggtacgg cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat
     8221 caaacatggt gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg
     8281 caggagctgg atgcggacaa taaggtcatc accaaagtct gggacggcaa acaggatatt
     8341 tatcacctgc tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca
     8401 gcggttgcag cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg
     8461 ggttttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt
     8521 ttgggtttga catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc
     8581 aaatcaccaa tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg
     8641 cgaccattgt gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag
     8701 tcatcccgca caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt
     8761 ggccgaaccg gatcgccaac ggctgttacc gttatcaggg gcaggaatat cagttaccca
     8821 ttaatgaaca tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa
     8881 ttagtgaatt aaccaccacc agcgttaccc tgacagcttt tctgccgccc agttacggct
     8941 atccgtttat gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg
     9001 ttgagatcgc aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcatc
     9061 cttatttaac ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc
     9121 aggtttatgc cgtcgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg
     9181 atttaaattt cacgcaggcg aaaaaaatag ccgcgacaaa aatcgaccac acctttaaaa
     9241 ccgctaatga tttgtgggaa atgaccatta cccatccaca gcaggcgctt tcggtaagcc
     9301 tttgttccga tcaaccctgg gtgcaggttt atagcggtga aaaattacag cgtcagggac
     9361 tggcagttga acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat
     9421 tactggaacc aggaaaaccg caccgcctgt tttttaatat ttacggacag agaaaataac
     9481 ttgtattgat tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat
     9541 gtatcttcaa tttattaagt taaaaacagc tcagcttctg taaagttttt atatatcagt
     9601 tttatgagaa tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa
     9661 ctctatggag attagttatg gatacgccac gtccacagtt attagatttt caatttcatc
     9721 agaataacga cagttttacc ctacattttc aacaacgtct tattttaacc cataacaaag
     9781 ataatccttg tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt
     9841 tcagcattaa agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt
     9901 caccggatgg ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata
     9961 tctctgccga cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca
    10021 accgtatctg gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac
    10081 agttttccta cttcgatctg cgcggcaaac cgttcccgct atggaccagt gaacaaggcg
    10141 ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg
    10201 gcgactatta ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact
    10261 gccatgttga taacagttgc tacatgaact tcgactttag cgccccggaa taccatgaac
    10321 tggcgctgtg ggaagacaaa gctacgctac gttttgaatg tgctgacaca tacatctccc
    10381 tgctggaaaa attaactgcc ctgctgggac gccagccaga actgcccgac tggatttatg
    10441 acggggtaac gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca
    10501 tgcgtaacgc gggcgtgaag gttaacggca tctgggcgca ggactggtct gggatccgta
    10561 taacctcttt tggcaagcgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc
    10621 aactggattc acgcattaag cagtggtatc aggagggcgt gcagttcctg gcctatatca
    10681 acccgtatgt tgccagcgat aaagatctct gcgaagaagc ggcacaacac ggctatctag
    10741 caaaagatgc ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg
    10801 tcgatctcac taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga
    10861 ttgaactcgg ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacacgt
    10921 acctgcataa cggcgtcagt gccgaaatta tgcataacgc ctggcctgcg ctgtgggcga
    10981 agtgtaacta cgaagccctt gaagaaacgg gcaagctcgg cgagatcctc ttctttatgc
    11041 gcgccggttc taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg
    11101 tggactggag tctcgacgat ggcctggcgt ccgtagtccc ggcggcgctg tcgctggcaa
    11161 tgaccggaca tggtctgcat cacagcgaca ttggcggtta caccaccctg tttgagatga
    11221 agcgcagcaa agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc
    11281 gcacccacga aggtaaccgt cctggcgaca actggcagtt tgacggcgat gcggaaacta
    11341 tcgcccattt tgcccgtatg actaccgttt tcaccaccct gaaaccttat ctgaaagagg
    11401 ccgtcgcgct gaatgcgaag tccggtctgc cggtcatgcg cccgctgttc ctgcattacg
    11461 aagacgatgc acatacctat tccctgaaat atcagtacct gttaggccgc gacattctgg
    11521 tcgctccggt acacgaagaa ggtcgcagcg actggacgct ctatctgccg gaggataact
    11581 gggtacatgc ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca
    11641 tcggcaagcc gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt
    11701 taaacagcat ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga
    11761 tgagtcacat cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct
    11821 cttacggtat tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt
    11881 tgaagtttta taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga
    11941 tttcaaaatt ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac
    12001 gcaagatcgg tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca
    12061 ccttactggc gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga
    12121 tggccactat tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg
    12181 gcgcaatggt cccggcaatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc
    12241 gtcagggcgg cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagttatga
    12301 atcttatcga aggtaatcag aaacttggct atatcttcgc cgccacgctg ttttcactgt
    12361 tcggcctgct gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc
    12421 aaccaaccaa tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta
    12481 accgcccact gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca
    12541 agctcgccat tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt
    12601 atatgggatt tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag
    12661 tcagacgttt tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg
    12721 atctgctcaa ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg
    12781 cgttcttcgg ctcagcgcct gaactgggcg ctggtttccg acaccgtcga gtacggcgag
    12841 tggcgcaccg gcgtgcgttc ggaaggcacg gtctacaccg gcttcacctt ctttcgcaaa
    12901 gtgtctcagg cgctggctgg tttcttcccc ggctggatgc tgacgcaaat tggctatgtg
    12961 ccgaacgtcg cacaggctga ccacaccatt gaagggttac gccagttgat cttcatctac
    13021 ccaagcgcac tggcggtagt caccattgtg gcgatgggtt gcttctacag cctgaacgag
    13081 aagatgtatg tccgcattgt ggaagagata gaagcccgta aacgcacggc gtaataataa
    13141 taaacgacgc cctgcggggc gttataagga gtgattatgt ctgaccataa tccactgaca
    13201 ttaaaactga atctgcggga aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta
    13261 atgctctgca tcggtactct gtatctcctc aaactttata ccgatgaatt agggatgcct
    13321 gcttactatg gcgggattat ctttctggtc gcgaagtttt ttaccgcgtt taccgatatg
    13381 ctcaccggat ttttactcga ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc
    13441 tttattttat atgcagcagt tccggcggcg ttaattgcaa cgctccagtt tatcgccacc
    13501 accttttgtt tgccggttaa aacgacgatt gccaccacgc tgtttatgat gtttggactt
    13561 tcatacagtc tgatgaactg ctcgtatggt gcgatgatcc cggcaattac caaaaacccg
    13621 aatgagcgcg cgcagctcgc ggcttaccgc cagggtggtg caaccatagg gctattgatt
    13681 tgtaccgtgg cgtttattcc gttgcagtcg cttttttctg actcaaccgt cggttatgcc
    13741 tgtgcggcac ttatgttctc cattggcggc tttattttta tgatgctgtg ctacagaggc
    13801 gtcaaagagc attatgtgga cacagcgcca accggacata aagccagtat tctcaaatct
    13861 ttttgcgcga tatttcgtaa tccaccattg ctggttttat gcattgctaa cctgtgtact
    13921 ctggcggcat ttaatatcaa actggcgatt caggtctatt acacccagta tgtgctgaat
    13981 gatattaatt tattgtcgtg gatgggattc ttcagtatgg gatgcatcct cgtcggcgta
    14041 ttactggtgc cagtaactgt aaaatgtttt ggtaaaaaac aggtttattt agctggcatg
    14101 gtgctatggg cggtgggtga tatactgaat tatttctggg gaagtaactc tttcactttc
    14161 gtcatgttct cttgtgtcgc cttttttggc acggcctttg taaacagcct gaactgggca
    14221 ctggtaccag ataccgtaga ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct
    14281 gtttataccg gttatacctt ctttcgtaaa atttctgccg cacttgctgg cttcttgcca
    14341 ggcattatgc tgacgcaaat tggttatgtt cccaacatcg cgcaaagcga tgcgacatta
    14401 cagggtttgc gtcagctcat ttttatctgg ccttgtgcac tggcaattat tgctgcattg
    14461 acgatgggat tcttttacac actcaatgaa aaacggtttg cattgattat tgaggaaatc
    14521 aaccaacgca aaaataaaga gatggcgaca gaaaaaaaaa cggcttccgt aacgttataa
    14581 atgtcaatag ccgctatttc catcctggtg ggtggcggcc tccctacgtt taaaaaatgg
    14641 acttattatg aaaaagatta atgcaataat tttattatct tctctgacct ctgcctcggt
    14701 atttgctggc gcatacgtcg aaaacaggga ggcatataat cttgcctccg atcagggcga
    14761 ggttatgcta cgtgtgggtt ataacttcga tatgggcgcg ggtattatgt taaccaatac
    14821 ctacaccttt cagcgagaag atgaactaaa acatggctat aatgaaattg aaggttggta
    14881 tcctttattc aaaccaaccg ataaattaac catccagccc ggtggcttaa ttaatgataa
    14941 gagtatcagt tcgggtggtg cagtgtatct ggac
//
LOCUS       DAECOD010000092        14867 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.092, whole genome shotgun sequence.
ACCESSION   DAECOD010000092 DAECOD010000000
VERSION     DAECOD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.092"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KKO15_003009"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KKO15_003009"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBD6361717.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KKO15_003010"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KKO15_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBD6361718.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KKO15_003011"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KKO15_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBD6361719.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KKO15_003012"
     CDS             2977..3060
                     /locus_tag="KKO15_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBD6361720.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KKO15_003013"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KKO15_003013"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBD6361721.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KKO15_003014"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KKO15_003014"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBD6361722.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWQLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KKO15_003015"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KKO15_003015"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBD6361723.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KKO15_003016"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KKO15_003016"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBD6361724.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KKO15_003017"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KKO15_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KKO15_003018"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KKO15_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBD6361725.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KKO15_003019"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KKO15_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBD6361726.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KKO15_003020"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KKO15_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBD6361727.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KKO15_003021"
     CDS             13186..14385
                     /locus_tag="KKO15_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBD6361728.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3701 a         3782 c         3917 g         3467 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagctgc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattatgcat catttttagg taatgct
//
LOCUS       DAECOD010000093        14566 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.093, whole genome shotgun sequence.
ACCESSION   DAECOD010000093 DAECOD010000000
VERSION     DAECOD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.093"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..572
                     /locus_tag="KKO15_003022"
     CDS             <1..572
                     /locus_tag="KKO15_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBD6361729.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(634..1356)
                     /locus_tag="KKO15_003023"
     CDS             complement(634..1356)
                     /locus_tag="KKO15_003023"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD6361730.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1358..2529)
                     /locus_tag="KKO15_003024"
                     /pseudo
     CDS             complement(1358..2529)
                     /locus_tag="KKO15_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2556..4172)
                     /gene="bglH"
                     /locus_tag="KKO15_003025"
     CDS             complement(2556..4172)
                     /gene="bglH"
                     /locus_tag="KKO15_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBD6361731.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4258..5652)
                     /gene="bglB"
                     /locus_tag="KKO15_003026"
     CDS             complement(4258..5652)
                     /gene="bglB"
                     /locus_tag="KKO15_003026"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBD6361732.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5671..7548)
                     /gene="bglF"
                     /locus_tag="KKO15_003027"
     CDS             complement(5671..7548)
                     /gene="bglF"
                     /locus_tag="KKO15_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBD6361733.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7682..8503)
                     /gene="bglG"
                     /locus_tag="KKO15_003028"
     CDS             complement(7682..8503)
                     /gene="bglG"
                     /locus_tag="KKO15_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBD6361734.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8790..9515)
                     /gene="phoU"
                     /locus_tag="KKO15_003029"
     CDS             complement(8790..9515)
                     /gene="phoU"
                     /locus_tag="KKO15_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBD6361735.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9530..10303)
                     /gene="pstB"
                     /locus_tag="KKO15_003030"
     CDS             complement(9530..10303)
                     /gene="pstB"
                     /locus_tag="KKO15_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBD6361736.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10395..11285)
                     /gene="pstA"
                     /locus_tag="KKO15_003031"
     CDS             complement(10395..11285)
                     /gene="pstA"
                     /locus_tag="KKO15_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBD6361737.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11285..12244)
                     /gene="pstC"
                     /locus_tag="KKO15_003032"
     CDS             complement(11285..12244)
                     /gene="pstC"
                     /locus_tag="KKO15_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBD6361738.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12331..13371)
                     /gene="pstS"
                     /locus_tag="KKO15_003033"
     CDS             complement(12331..13371)
                     /gene="pstS"
                     /locus_tag="KKO15_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBD6361739.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13619..>14566)
                     /locus_tag="KKO15_003034"
     CDS             complement(13619..>14566)
                     /locus_tag="KKO15_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6361740.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3754 a         3717 c         3320 g         3775 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggtgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag
      601 cgtaattcat acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc
      661 ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag ctggaacatc
      721 ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac
      781 aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc
      841 aggcactaac gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc
      901 ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca
      961 aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag
     1021 tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat
     1081 ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt
     1141 gccgcgaaag ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc
     1201 tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc
     1261 caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct
     1321 catttcctgg taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct
     1381 gtacgaaggc tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt
     1441 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
     1501 tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa
     1561 tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa
     1621 caccactgaa tgtggccagc caaagctttc cagatgattc attccggaaa ccagcgcctg
     1681 gtacccgcct tgtgaaagcc ctgccagcgc gcgaccatcg gcatctttac ggacactaaa
     1741 acgcttgcta atcagcggga taatatcgtt catcagttcg cgatctgccg ctttagcatt
     1801 cagcggataa aagactttac gtctttcctg aggcacgaaa tcttcgggaa taatgccctt
     1861 cgcatcggtt tcggtatccg ggatcaccac cagcatcggt ttaattttcc cttcagccag
     1921 caggttatcc atgatttgcg ggatacgccc ctgatcgata gcggaacgtc cggtatcacc
     1981 aaaaccgtga tagaaataga gcactggcaa aggctcgccc atgccggtgt atcctggcgg
     2041 ggtccagaca tacatctgac gttcagattg caatgcgttg gagtggtaag ttatggcgat
     2101 caaatcacca tgcgcaacag aacgcgtatc cagataactg cctggcacca gaatcatact
     2161 ggagttaacc tggcgctgag gcttagtcat tgcggtgcct gtatcaatgc tgcgtacacc
     2221 atcaacattg aaaaaatact cgtacaggtt gcctttcagg ataggtgtgc gccacgacca
     2281 gactcctgct tcgtctttgg tcatcgggtg aatattgtcc ggaaccggaa cacccaccac
     2341 tacagagaca ttttttgccc caggggcaaa gtagcgaaag gtcacgctgt tatcggcatt
     2401 gacctgagtc acatactgtt tcaccgggat agtgggtgcc gggctggctg gcatatccgc
     2461 tgctatggcc cactgagcag aaataccgct ggcaatagcc agcgttaaag cagcaatttt
     2521 tatattcatc gtaatattcc tttcattaag ttccattacc accagatttc agcctgggca
     2581 ccgacagcaa actgggcgtc tttattgtct tcgaaggtga agccatccag ttcgttttcc
     2641 agggctttga tatacgtggc gtagaaacga atttccagac gcgaggtcaa catactggta
     2701 ttgactttaa aggtatggaa gagcgtggtt ttataaccag actcattaaa tttattacta
     2761 ttcgcatctt tgttttgctg ggtgaaatag cccagttcaa cacctgtctg gttatattgg
     2821 tcccaaatat aggccgggcg aacaaccgca cgaatagatt cgaaatcaga gtgtgcgcct
     2881 gtttcgtagc tataaatatt gttaccgaag gagtaaacaa tcgcgttagc cacaataaaa
     2941 tgatcgccga tataggcttc gccctgcgag gtcagacgaa ctgctgttcc gccggtgtga
     3001 tcaccataat aacgaccatt aaaggtggta aatggacttg cgccagcata acggccaaag
     3061 ttcctggcga tagagttatt cgcaaccagg aaggagaatt cgttgaagcc acctttatca
     3121 aatttctgcg ttaaagatgt gccgaacatc caggtatctt tccagtcata atacccgtta
     3181 ttatcctgat tatctttttc cgatgcgctt tcgtttgccg tgacataacg accactcacc
     3241 attaaggtcg ctttatccca taacgggata tctttatagc gtaaatcaat agtatgggta
     3301 ttaatctgct gtttgttttg caggctgcga tcgtaatcat cgacatcttc gcgaaccagc
     3361 gcgatatcaa ttttacccgg accgactttc cagttttcca gacctacacc cgctgcagca
     3421 tcagtacgct gcgttttcca gtccagcatc tggatttcaa ttttcggcgc accgtgttta
     3481 cccacccaga aatcagcctc tggcgcaaag ggcaggaaac ctttggtggt aacgtacata
     3541 tcggagaact gcataaagtt ctcgccaccg gcattatcgc caaaccagcc ggtagagtac
     3601 tgctgaccaa cgttaccatc tatcatcaca acggcatcaa cccgtttgcc gttttcgttg
     3661 tagacacgtt gttttaactg caaatcaaac cagccggagt attcgttacc aaagcggccc
     3721 agagaaccaa tcgcccatga tttaggtgaa ccatgagagg cggtccccca accagaacgg
     3781 taatagccgt tgtaactaaa gccaatttca tctttcacaa acttactgaa atctttcaga
     3841 gtcatggaag aataaatcga gcctgtctca ctggcatttt tctcttcatt tttgaccagt
     3901 acagcatcag gttttgcggc actactggtc gggaacggat tggcggcata cccttgatca
     3961 ttcgtactta cgctacgatt caccgtcgct ggcgtatatt ttttcttctc ttcatcttta
     4021 tactttttga gttcgctttg cgtttctctt aacgcttttt ctaataactc aaggcgttgt
     4081 tccaccgtta atgattcagc caatgattgt gcagaaaagg ctaacggtgc cattaataag
     4141 atggcagagg taataagatt tcgtctaaac ataatatccc tttatggtgc aaagaaagaa
     4201 ttaacgtatc gcatccagac tgttctgaat gcgacgataa ttaaggtgct ttattggtta
     4261 tttttttaat gacagccccc gcgttttgat cacttctgca taccatccga agcttttctt
     4321 acgcgttctt gtgaggcttc cttcgccatt atcatcacga tccacataaa taaagccgta
     4381 gcgcttagac atttgtgaat gagatgcact gactaaatca attggccccc aactggtgta
     4441 ccccataata tccacaccat cggcaatcgc ttcatttacc tgtaccaggt gatcgtttaa
     4501 ataggcaatt cgataatcgt cctgtatcga accatccgct tcaacgctgt cttttacgcc
     4561 taatccattc tcgacaataa ataacggttt ttgataacga tcccaaagcg tatttaacag
     4621 aacccgtaat ccaaccggat caatttgcca cccccactct gaacttttca gatgcggatt
     4681 ggggatcata ttcagtatgt tgccctgcgc atttttatta atgctttcgt cgtgggaaac
     4741 acaaccagtc atgtaataac taaaagagat gaaatctaca gtatgtttta aatcttctgc
     4801 gtcactttca gtcatctcaa tggtgatatt gtggtcgcgg aagaaacgct gcatatagcc
     4861 gggatattgg ccacgcgcct gaacatcacc aaagaacatc cagcgccggt tctcttccat
     4921 ggcctgcaac atatcctgtg gctggcaggt gagggggtaa accagcccac cgagaagcat
     4981 attgccgatt ttcgcttcgg ggatcaggct atgataggct ttaactgccc gcgcactggc
     5041 aaccagttga tgatggatcg cctgataaac ttccgcctcg ccactctctt ctgccagccc
     5101 cacgcccgtg aatggcgcgt gtaacgacat attgatttca ttaaacgtca gccataacgc
     5161 tactttatgt tggtagcggg taaagaccgt gcgggcgtaa tgctcgaagt gatcgatgac
     5221 cgcccgatta gcccaaccgc cgtagttttt caccagccca tatagcattt cgtaatggga
     5281 taacgttacc agcggcttga tccccgcctg cgccatttca tcaaacagcc gatcgtaaaa
     5341 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
     5401 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
     5461 acggtaataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
     5521 catttttccc attacgccat gaggctgtaa atctgaggtc gtgatccctt tgccatcttc
     5581 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
     5641 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
     5701 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
     5761 taataccggc gtcgtcagat caaatccggc ctcgcgaata gcagggatat caaaagaaat
     5821 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
     5881 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
     5941 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
     6001 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
     6061 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
     6121 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
     6181 acgtttactg gtgatgaaat gaagcatcaa cgtaccaaca aatgcgcaac ctatggcaat
     6241 gacaccgcca ataacgctgg cccagacggt gaaatcaatc cccgttgacg ggatggtctg
     6301 catgaaggtg aaaatacttg gcaaaccaaa ggagtaaact ttcgtttgcg cgtagccaat
     6361 gatggtggcc cccaatgctc cactgataca ggcgataaca aaggggtact tacgcggcag
     6421 gttgacgcca tataccgctg gttcggtgat accaaacaga cccgtcaacg ccgctgatcc
     6481 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     6541 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     6601 gttattgata cacagcggca ccaggcccct gtgcagtccg aacatgacga agatttgcca
     6661 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     6721 ggcggcaatc atttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     6781 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     6841 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     6901 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccggcgcatc
     6961 cgctttttgc ccgttctcga aggcagtcag aattaatgga tgcactaacg ctccaccaat
     7021 caccatggca gtaaatggat taccgccgaa gcgtttgccc gcggtgtatc ccaggattat
     7081 cgggaagaac ccaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     7141 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     7201 cgccatcaga ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     7261 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     7321 gctgttaacc gccaggaaga catcggccac atggttacct atgaccacct gaaactgacc
     7381 accgctttcc accaccataa taataccggg ggtctttttc agtacctcag cttgcgcttt
     7441 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac tcacaatgtt
     7501 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     7561 accgctttgc ggcaaaactc caaaaaaaaa cctgaaaaaa acggcctgac gtgaatcaag
     7621 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     7681 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     7741 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     7801 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     7861 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     7921 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     7981 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     8041 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     8101 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     8161 gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat
     8221 aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataaga cgatcacagg
     8281 ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg ttcagttcat
     8341 gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg ccagcgcgtt
     8401 tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca tcaataacca
     8461 ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta
     8521 taaacaaaaa aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
     8581 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
     8641 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
     8701 gcagttatta actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat
     8761 aattaagtta ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt
     8821 ttatccagct catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata
     8881 aactcacaaa tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc
     8941 gcggtaagta cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga
     9001 acaatacctt cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg
     9061 tcaatgtcca tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg
     9121 cccagcgact ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca
     9181 gtacggcaga ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg
     9241 atcaccataa ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg
     9301 caggcttcat cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc
     9361 ttcgccagat cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc
     9421 accatgccgc ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg
     9481 ccggaaatat gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac
     9541 gaccggtgat gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt
     9601 tgctgaactc aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag
     9661 cctgctgcat gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca
     9721 gctcttcaat acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca
     9781 gcacttccgg gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag
     9841 agagagagta accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg
     9901 tcaatgccca ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa
     9961 cgccaaaggc gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca
    10021 tgcccacttt cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat
    10081 caagcagaat ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg
    10141 tacgcagcag cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt
    10201 tggcgatatc caagttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat
    10261 tacgaacctg aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat
    10321 cgtacctgat gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg
    10381 catccggcaa agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc
    10441 agcagcagga cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc
    10501 gcaaacgggc tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc
    10561 atgtccgtgc tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg
    10621 gcaatacggg caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc
    10681 gtaatcgcgg agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg
    10741 ctgtccggca ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac
    10801 agcgccagcg caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg
    10861 gtgtagacaa acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag
    10921 cgaatcactt ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata
    10981 atgcccagcg gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg
    11041 gcgttcgcca gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc
    11101 agcgacatac cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac
    11161 aggccgaagg ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga
    11221 cgcgcctgca ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata
    11281 gccattagcg tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga
    11341 ggacgatgaa ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca
    11401 gaccggattc cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca
    11461 tgtacagcga ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg
    11521 tttcaccgag cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga
    11581 acggaagaac gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact
    11641 ctttcatcat caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa
    11701 taatcatgat ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag
    11761 agaacagcgc gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt
    11821 aaacggcgaa cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa
    11881 tggctgccag cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct
    11941 cagtcaggaa cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg
    12001 aagtcaccaa cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc
    12061 actctttggt ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga
    12121 tcagagagac aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca
    12181 gcacgctgaa aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag
    12241 ccataagtta ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc
    12301 gtaaaacacc gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt
    12361 aatattggtc ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta
    12421 atccaggtcg ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac
    12481 ctctgtgcct tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt
    12541 aataggccat gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca
    12601 gtctgcacct tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc
    12661 agcggagatc agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata
    12721 accaattgca cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc
    12781 cagaccgatc ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt
    12841 cactttcgcc aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac
    12901 tacagcaatg ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc
    12961 atcccacttc ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag
    13021 ttcgccagac ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg
    13081 gaactggaac agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc
    13141 aaaatcaacg gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta
    13201 gttaacttta ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc
    13261 agggaaggtt gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat
    13321 cgataaggtc gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc
    13381 tgggaggatt cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg
    13441 acagttatat ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac
    13501 tgattttaaa tgtgaagatg aaaatcccac atgccatctt tttgtcatga tgcatttaaa
    13561 agtgataaag aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt
    13621 agcgcacaat ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag
    13681 ctccagtaag gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca
    13741 ctgttgattt atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat
    13801 ttttgtcgta aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg
    13861 tattgggttc ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt
    13921 tttcacactc aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa
    13981 cagatgaaaa atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat
    14041 ttatttcgca actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct
    14101 gtgagtaatc caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt
    14161 caggaataat caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag
    14221 ccccactgga tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg
    14281 ggttgttagc atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag
    14341 caatcccaac atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag
    14401 cataagttaa tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt
    14461 taccagtatc acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa
    14521 atgtcgttcc aaccttattt tcgttcgcgg taataagata tcccga
//
LOCUS       DAECOD010000094        14479 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.094, whole genome shotgun sequence.
ACCESSION   DAECOD010000094 DAECOD010000000
VERSION     DAECOD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.094"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(113..379)
                     /locus_tag="KKO15_003035"
     CDS             complement(113..379)
                     /locus_tag="KKO15_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBD6361741.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="KKO15_003036"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="KKO15_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBD6361742.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="KKO15_003037"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="KKO15_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="KKO15_003038"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="KKO15_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBD6361743.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="KKO15_003039"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="KKO15_003039"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBD6361744.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KKO15_003040"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KKO15_003040"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBD6361745.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KKO15_003041"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KKO15_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBD6361746.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="KKO15_003042"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="KKO15_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KKO15_003043"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KKO15_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBD6361747.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="KKO15_003044"
     CDS             complement(12641..14095)
                     /locus_tag="KKO15_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBD6361748.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="KKO15_003045"
     CDS             complement(14092..>14479)
                     /locus_tag="KKO15_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6361749.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3652 c         3421 g         3546 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAECOD010000095        14451 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.095, whole genome shotgun sequence.
ACCESSION   DAECOD010000095 DAECOD010000000
VERSION     DAECOD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.095"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(337..582)
                     /locus_tag="KKO15_003046"
     CDS             complement(337..582)
                     /locus_tag="KKO15_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361750.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="KKO15_003047"
     tRNA            complement(1227..1302)
                     /locus_tag="KKO15_003047"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="KKO15_003048"
     CDS             complement(1408..2115)
                     /locus_tag="KKO15_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBD6361751.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="KKO15_003049"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="KKO15_003049"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD6361752.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KKO15_003050"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KKO15_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBD6361753.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KKO15_003051"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KKO15_003051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBD6361754.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="KKO15_003052"
     CDS             complement(7299..7574)
                     /locus_tag="KKO15_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBD6361755.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KKO15_003053"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KKO15_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBD6361756.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="KKO15_003054"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="KKO15_003054"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBD6361757.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="KKO15_003055"
     CDS             9534..9860
                     /locus_tag="KKO15_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBD6361758.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="KKO15_003056"
     CDS             10044..10763
                     /locus_tag="KKO15_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBD6361759.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="KKO15_003057"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="KKO15_003057"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBD6361760.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13064
                     /locus_tag="KKO15_003058"
     CDS             12102..13064
                     /locus_tag="KKO15_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBD6361761.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13165..>14451)
                     /locus_tag="KKO15_003059"
     CDS             complement(13165..>14451)
                     /locus_tag="KKO15_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBD6361762.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTT
                     SIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMT
                     PAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQH
                     LIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3791 a         3465 c         3713 g         3482 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc ataccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg catccagaaa
    13801 cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac ggcgtttagc
    13861 aataatgccg cagactatca ttaccgcaag acccattaag atccctggca aatatcccgc
    13921 cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa ttaacacgtt
    13981 tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg tagaaaatgc
    14041 cggatcataa ccttctttgg tctgaatggg attcagtgtg ccaccaacgg cagcagctgc
    14101 cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt tcacatgacc
    14161 aagcgaacca ggaacgcggc caaccattac ctgggcgagg ttaatcaaac gtatcgcaat
    14221 accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca acagggcaaa
    14281 gttatccagt ccattagcca gtcgttgcga aacagtgatt gccgcgatat cccagggaaa
    14341 catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg gcacaccaat
    14401 aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg atataactat c
//
LOCUS       DAECOD010000096        14318 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.096, whole genome shotgun sequence.
ACCESSION   DAECOD010000096 DAECOD010000000
VERSION     DAECOD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.096"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..3051
                     /locus_tag="KKO15_003060"
                     /pseudo
     CDS             1..3051
                     /locus_tag="KKO15_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="KKO15_003061"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="KKO15_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="KKO15_003062"
     CDS             6768..7175
                     /locus_tag="KKO15_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361763.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="KKO15_003063"
     CDS             7717..8805
                     /locus_tag="KKO15_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBD6361764.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="KKO15_003064"
     CDS             8816..11095
                     /locus_tag="KKO15_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361765.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="KKO15_003065"
     CDS             11088..12224
                     /locus_tag="KKO15_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD6361766.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="KKO15_003066"
     CDS             12221..14224
                     /locus_tag="KKO15_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361767.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3755 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacttggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatga tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgccgcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tattattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactccagga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctgatta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcacca
//
LOCUS       DAECOD010000097        13963 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.097, whole genome shotgun sequence.
ACCESSION   DAECOD010000097 DAECOD010000000
VERSION     DAECOD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.097"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KKO15_003067"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KKO15_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6361768.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KKO15_003068"
     CDS             2219..2767
                     /locus_tag="KKO15_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBD6361769.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KKO15_003069"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KKO15_003069"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBD6361770.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KKO15_003070"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KKO15_003070"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBD6361771.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KKO15_003071"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KKO15_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBD6361772.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KKO15_003072"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KKO15_003072"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBD6361773.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KKO15_003073"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KKO15_003073"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBD6361774.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KKO15_003074"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KKO15_003074"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBD6361775.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KKO15_003075"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KKO15_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD6361776.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KKO15_003076"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KKO15_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBD6361777.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3564 c         3525 g         3322 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgctacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAECOD010000098        13956 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.098, whole genome shotgun sequence.
ACCESSION   DAECOD010000098 DAECOD010000000
VERSION     DAECOD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.098"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="KKO15_003077"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="KKO15_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBD6361778.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="KKO15_003078"
     CDS             complement(1604..1777)
                     /locus_tag="KKO15_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBD6361779.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="KKO15_003079"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="KKO15_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBD6361780.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="KKO15_003080"
     CDS             3415..3744
                     /locus_tag="KKO15_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBD6361781.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="KKO15_003081"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="KKO15_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBD6361782.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="KKO15_003082"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="KKO15_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBD6361783.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="KKO15_003083"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="KKO15_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBD6361784.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="KKO15_003084"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="KKO15_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBD6361785.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="KKO15_003085"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="KKO15_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBD6361786.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="KKO15_003086"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="KKO15_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBD6361787.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="KKO15_003087"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="KKO15_003087"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBD6361788.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3659 a         3455 c         3246 g         3596 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatgcc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAECOD010000099        13798 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.099, whole genome shotgun sequence.
ACCESSION   DAECOD010000099 DAECOD010000000
VERSION     DAECOD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.099"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="KKO15_003088"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="KKO15_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBD6361789.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="KKO15_003089"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="KKO15_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBD6361790.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="KKO15_003090"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="KKO15_003090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBD6361791.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="KKO15_003091"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="KKO15_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBD6361792.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="KKO15_003092"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="KKO15_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBD6361793.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="KKO15_003093"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="KKO15_003093"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBD6361794.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="KKO15_003094"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="KKO15_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBD6361795.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="KKO15_003095"
     CDS             7490..7639
                     /locus_tag="KKO15_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361796.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="KKO15_003096"
     CDS             7840..9423
                     /locus_tag="KKO15_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD6361797.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="KKO15_003097"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="KKO15_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBD6361798.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="KKO15_003098"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="KKO15_003098"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBD6361799.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="KKO15_003099"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="KKO15_003099"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBD6361800.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..>13798
                     /locus_tag="KKO15_003100"
     CDS             13382..>13798
                     /locus_tag="KKO15_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBD6361801.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3194 a         3641 c         3493 g         3470 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccaac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgttgggg
//
LOCUS       DAECOD010000100        13571 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.100, whole genome shotgun sequence.
ACCESSION   DAECOD010000100 DAECOD010000000
VERSION     DAECOD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.100"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..495
                     /locus_tag="KKO15_003101"
                     /pseudo
     CDS             1..495
                     /locus_tag="KKO15_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(792..2243)
                     /gene="uxaB"
                     /locus_tag="KKO15_003102"
     CDS             complement(792..2243)
                     /gene="uxaB"
                     /locus_tag="KKO15_003102"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBD6361802.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(2470..3888)
                     /locus_tag="KKO15_003103"
     CDS             complement(2470..3888)
                     /locus_tag="KKO15_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD6361803.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4042..4386)
                     /locus_tag="KKO15_003104"
     CDS             complement(4042..4386)
                     /locus_tag="KKO15_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBD6361804.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(4386..5312)
                     /gene="glsB"
                     /locus_tag="KKO15_003105"
     CDS             complement(4386..5312)
                     /gene="glsB"
                     /locus_tag="KKO15_003105"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBD6361805.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5376..6311)
                     /locus_tag="KKO15_003106"
     CDS             complement(5376..6311)
                     /locus_tag="KKO15_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBD6361806.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            6391..7506
                     /gene="yneK"
                     /locus_tag="KKO15_003107"
     CDS             6391..7506
                     /gene="yneK"
                     /locus_tag="KKO15_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBD6361807.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     ELQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRPLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            7656..8846
                     /gene="ydeA"
                     /locus_tag="KKO15_003108"
     CDS             7656..8846
                     /gene="ydeA"
                     /locus_tag="KKO15_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBD6361808.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8871..9536)
                     /gene="marC"
                     /locus_tag="KKO15_003109"
     CDS             complement(8871..9536)
                     /gene="marC"
                     /locus_tag="KKO15_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBD6361809.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9748..10182
                     /gene="marR"
                     /locus_tag="KKO15_003110"
     CDS             9748..10182
                     /gene="marR"
                     /locus_tag="KKO15_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBD6361810.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            10202..10585
                     /gene="marA"
                     /locus_tag="KKO15_003111"
     CDS             10202..10585
                     /gene="marA"
                     /locus_tag="KKO15_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBD6361811.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            10617..10835
                     /gene="marB"
                     /locus_tag="KKO15_003112"
     CDS             10617..10835
                     /gene="marB"
                     /locus_tag="KKO15_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBD6361812.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10866..11765)
                     /gene="eamA"
                     /locus_tag="KKO15_003113"
     CDS             complement(10866..11765)
                     /gene="eamA"
                     /locus_tag="KKO15_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBD6361813.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            12029..13147
                     /gene="ydeE"
                     /locus_tag="KKO15_003114"
     CDS             12029..13147
                     /gene="ydeE"
                     /locus_tag="KKO15_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBD6361814.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            13274..13369
                     /gene="mgtS"
                     /locus_tag="KKO15_003115"
     CDS             13274..13369
                     /gene="mgtS"
                     /locus_tag="KKO15_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBD6361815.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3573 a         3446 c         3171 g         3381 t
ORIGIN      
        1 atgtgtacat gtcgaaaaaa acggccggcg ggaggaatac ggcgcgctac ctgggtcgtt
       61 atctgaagaa gccgccgata gcggcctccc ggctggcaca ttacaacgga gggggagcct
      121 gagcttccgt tacctggacc acaaaacggg agaaacggcg acggaaacgc tgacacagcg
      181 tgagctggtc gcgaggctga aacagcacat cccggagaag tttttaagat ggtgaggtac
      241 ttcgggttcc ttgccaaccg ggtgtgtgga gagaagctgc cgcaggtgta ccgtgctctg
      301 gggatggata aaccggaacc agtggcgaaa gtgtgctatg cacaaatggt gaaacagttc
      361 ctgagtcgtg acccgttcga atgcgtgctg tgtggcggcc ggatggtata ccgccgggcc
      421 atcgcgggac tgaatgtgga ggggctgaag aaaaacgcgc gggatatcag tctgctgagg
      481 tatatgccgg cctgacacag gctaagtgtg cccgggaatc ggtgcaagta taaaaaacag
      541 cctgttgcgg tataagaatc gaccatgcag agagagaaaa caccggagtg gagggaaaag
      601 cagaaaagct cacgtggcat aaggcgtgga cagcggtatc gtctggattt tcaatttcct
      661 atatgtcaag cttacaacat taacaggcgc tgcatgtgac acctgttaat gataaaggga
      721 tataggatgt tgtaactaac atagtgaaat aagaaacccg gtcgaaaccg ggttcagaag
      781 taacggtgtt attagcacaa cggacgtaca gcttcgcgca tccctttttc aagaatcgca
      841 tccaggtcat tagcaacctg ctcgaccaga ccaggcactt gcgtcaggtc ctgctcccag
      901 tggtcttttt ctgccaacac aatcgctacc agttcctggg taccgataac acggtcacga
      961 tgctggctcc agagttgctg ataacgttcc agccagtgtg catcgtcctg taccggatat
     1021 gtttctccgt tacgctcacc acgatagaac gcaatcaatg ccgctaatgc gaaagtaagg
     1081 cgcgccggaa gtgtgccatt tgccttctgc cctgccagca gctgcggcag gatgcgggtg
     1141 cggaacttgg tcataccgtt gagcgcgata gacagcagct gatgcttaat gtacgggtta
     1201 cggaaacgcc cggtgactgc actggcgaaa gattccagtt catcacgagg caaatccagt
     1261 accggaataa tttcttcgta gatggctttt tcaacgaatg cgcaaatttc agcatcgttc
     1321 atcgcttcac ctacggtatc cagccctgcc tggaacgcca ccggcaccag cgcggtgtgc
     1381 gcaccgttga ggattgccac tttgcgttct ttatacggtt taatatcgtc aacaatcagc
     1441 acgttgagcg gatatttgtc caggcgcagc tcagtcgcta aggatttcgg cccctgaatc
     1501 acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat cgtgataacc cagttcttct
     1561 tccagtttag ccacttcatc gcgcggataa ccggtaacga tacggtccac cagcgtagaa
     1621 cagaagctgt tagcctgatc cagccattga ataaatgctt ctggcagtgc ccactcttgc
     1681 gcatagcgca gcaccagttc acgcaacgcg tcgccattgt agtcaatcaa ctcacacgga
     1741 atgatgatcc aacctttatc cagcgcaccg ttgaaatggc taaagcgttc gaacagcaga
     1801 cgggtcagtt ttgccggata gcttaccgct ggcgcgtcat cgaatttatc gcccgcatgg
     1861 tagctgatac ccgcttcggt ggtgttagag aaaacaaagc gcatttccgg gttgtgcgcc
     1921 agtttcagga attcatcgta ttcactgtag acgctgattt cacgattaac cgagcgaatc
     1981 agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc cacggataat ggtggtgtac
     2041 agaccatcct gcgtgctcag tgacggcggg aatgaagttt caatcggacg aacaacgacc
     2101 acgccagaat tcagatcggt gtgctcattc aggagatcga tttgccagtc aacaaaggcg
     2161 cgcaggaagt taccttcacc aaactgaatg atacgttctg gatactgtgc accgggaaaa
     2221 tcgcgacgat ttagtgtttt cacaatgggt tcccttctga ttagtcatac aacctgtttg
     2281 aattggtgcg acaggttagc aaactttaat acgccgaacc cctgttttga tcaactcctg
     2341 atgattaatg agcagtttta tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa
     2401 tacgcctttt cctggatcat aaagtggtag aacacattgc attcaaatcg cgcgaaatga
     2461 ataaagatgt cagacaactt cctcaccgta acgcatagtg ctggtacggt tgcgcccatc
     2521 tttcttcgag cgatacagac acgtatcggc ttcgaccatc aatttattaa aatcatcggt
     2581 cagcgttcgg tacgatgcgc gaccactacc gacgccaata cttaccgtga gataaagcgt
     2641 tttttgttgc caggtgaatg gttgcagttc aacgccttta cggatttttt ccgccattag
     2701 cagaccatcg acaggattca ccgacggcac tgcaacagca aattcttcgc cgcccatgcg
     2761 cgccaccagc cccttatcac cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac
     2821 tttatcgcca cattcatgtc catagttatc gttaatgctt ttgaaatagt cgatatcaag
     2881 cagcatgacc gtcagatgtt gtgtctgttt cagcgatgga cttttcagcg cctcataaag
     2941 accggaccgt gagtaaacct gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt
     3001 cattagcaaa ttgatcgctg ccacgctaaa agaaaccata attgggcata tcgccatcgt
     3061 ggcaataccg agacgtgcgg agaacatttc cggaatggag aacggcgaac cgaccgagat
     3121 atcaatcacc gaatttgcca ccagtacgat ttccaccgca ccggtgacaa aggtcaacag
     3181 acatgttacc tgcggcgtat agcgcactgc acaccagatt aatgcaggca gcgggaacgc
     3241 cagactcccc gctccgccaa tgactaccga ggcaataacc gacacaataa gcgcgatggc
     3301 gggcatcatc tgttctgctt taaagcgcgg cagcactcca ggaatagcca acgtcagcat
     3361 acaaggcacg atcaacacgc ccgttgagaa ttgctcactg aaccagtccg caagcaaagg
     3421 ccagaaatcc agactatcaa tactgaccga accaatcgcc ccgacaatag cgcataataa
     3481 tgcggcaatc agacagtaat taaatagccg taaagcactt actggctcat acttattttt
     3541 ccccagacgc ttatcccgca cgaccagtaa ggcgacggta acaataaaca tcatattgga
     3601 gaaattgata accagtgaaa ccagtcccca ttcggtggtt atggcatcat aaaccagcat
     3661 cgccacataa ctgatcgcat agtaatgcag tcgattaagc cagacatagc gggcaaacac
     3721 ccccgccatt acgccattca gcggccagaa aagagaaagt tcgtgaacca gccgtaactc
     3781 tgcaccaata aaatagaaca gcgtggttag cgcaaaaata gcgatgctat tacgtagcag
     3841 atgaccttcc tggaacaaac ggaaagttga aatggggtgt acatgcatta attatctgtc
     3901 catacaattg cgggtggcaa ttatctcaac gtgataagta aattctcata aaggatagca
     3961 tgttcgctaa cagcggcggc aaataaaggt accctgggaa tgctatttta tcgttttccg
     4021 caattctcat ggctgattca tctatacaac cagccagtga tagattaccg ccacgatata
     4081 acgttgttgt tcttcgctta acgaaacacc ctgcggaatg ttatgccact tagccagaca
     4141 accgcgacag caggtcgcgg tggcgtgctg agcgataaac accggatgcc cgcgcatggg
     4201 ggtttgctta ccgtcattag ccggtaatgc cggagcaaga cgtttagcga caaaatccgc
     4261 cgcatgttgt tcgatgactg gcgcgccttt ctccaggcaa tactgacgct ctttcatgcc
     4321 cagacgaaag cgagagcgaa attttgaacg ggataaacgc gcgaagagtg gatcgagaga
     4381 ctgcattaat aaaccgaacg ccctaactgt ttcgtcaatt gttcaaggac ggcgataccc
     4441 gcaagcgagt tacctgcatc atccagttcc ggactccaga ctgcgatggc catttcatgc
     4501 ggaacaatcg ccacaatacc gccaccaacg ccagatttcg ccggtagtcc cacccgccag
     4561 gcaaactccc ccgcgttctg gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc
     4621 gcctgcattg gcgtcaccac aggttcatca atatgaatag ctttcccctg attagccaga
     4681 aagacaaacg tccgggccag ctctacacag ctcattttca gagcgcagta atgaaagtag
     4741 ttttgcagaa cggttgtcac gtcatgatgg aaattgccaa acgacttcat cagccaggcg
     4801 atagccgcat ttcgcgcgga atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa
     4861 atatcagaca caccgcttaa gccgcgcacg acttccagca tacgttgccg tggtgcgctt
     4921 agtcgccctt gcaacatatc gcagaccacc agcgcaccgg tattaatgaa cggattacgc
     4981 ggaataccct gttccatttc cagttgcact aaagaattga acggtgatcc agacggatct
     5041 ttgccgacgc gttgccagat ttcctcttcg gagtaatgac gcatggcgac aacgagactc
     5101 agcactttgg aaatagactg aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc
     5161 tgtccgtcaa cggtacagat agcaatcccc aatcgggaac cgtctactgt agccagcgcc
     5221 ggaatataat ccgcgacttt accctgacca atgagcggcc gcacttgccg caagatgttt
     5281 tctaaaattg cattatccat ggcgactgcc actttctact cctggaccgc aggtctgaaa
     5341 agacctgcga gtatatcaga gctgaatatg tcgcgtcaga tccggtcttt ccacactgtc
     5401 tggatattac agaattcgtg taagccgaaa tgggaaagct cacgaccaaa gccactcttt
     5461 ttcacgccac caaaggccac tcgcgcgtcg ctggcacaat aaccattgat aaacacccca
     5521 ccgcattcca gacgtgccgc catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc
     5581 gctgaaaggc cgaactcact atcattagcc agttccagcg catgttctgc atctttcgca
     5641 atggtgattg cagcaacggg gccaaacatt tcttcccgaa acgcggtcat ttctggggta
     5701 acattcgcca gaaccgttgg cggatagtag ttacctgctc cagccatctt ttccccgccc
     5761 agtaacaaac gcgcaccctg cgccagggtt ttctccacct gatgatgcag ctcatcacgt
     5821 aaatcaaaac gagccattgg tccgagagcg ttctcttcat cacggggatc gcccattttc
     5881 aaggctgccg cagctgacac aaaacgttcg gtaaatgccg aagcaattcc ctcttcgata
     5941 ataaagcgtt ttgccgctgc acaaacctgt ccggtattct gataacgtcc ggctaccgcc
     6001 gctttaaccg ccagttccag atcggcatcg ttaagcacaa taaatggatc cgaaccgccc
     6061 agttccagta cgcatttttt cagtgccgct ccagcacgaa cacttccggt caccgtgaca
     6121 gcagcaatgc gcgagtcttt aatcatctga ctgacaccgt cgttgtcggc attcagccag
     6181 ccatatacgc cttgcgggat tcccgcatct ttaaacactt gggcaatgag ctgagcacag
     6241 cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaaca
     6301 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
     6361 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
     6421 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
     6481 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
     6541 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
     6601 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
     6661 tatcctgtca agaatggaag acacaaagaa aatattgaac ttcaggtagt aataaattta
     6721 agccctattt atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc
     6781 aacccaaagc ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt
     6841 ctgtttatga atgattttat tgaacaattt ccaatgatca accttggcgc ccccatcaaa
     6901 agagcgcata caccacatat tgaacccctg cctccggatc accataccgc tgcagattac
     6961 ttacgccagt ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc
     7021 ccccgattat ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg
     7081 atcaccgcca ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg
     7141 atggattcat taggttctaa caaacatgta caacccgaag tctgcatctg cttcttatcc
     7201 cttttagcag aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg
     7261 ttattgcact atcaccaatt tatgaatccc aatgaaagtg atctgaatga cgtgttgatg
     7321 ccagcatcat taagtgatga taaaattatc aaacatatgg cgcgcaggcc cctcaaactg
     7381 tttgtaaaaa atgaaacgcc gccaaaagtt actcacgaag acctggtgaa aaacaggcct
     7441 cgctcccctg tcagaccacc tatacccgca accgccaaaa cgccagacct ccctgaacgt
     7501 cattaaaccg tgatgttacc gactctctga ctcggaacag aatcagcgtc agagaaacgg
     7561 aaaacgcgat ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt
     7621 tcatcacttt ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca
     7681 aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg
     7741 aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc
     7801 aggtcggcat catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta
     7861 tgttaatgac cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta
     7921 ttgccagcca cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca
     7981 ttggtgtggc ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta
     8041 tggctccggc cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg
     8101 cgatggtctt aggtttaccg ctcgggcgca ttgtggggca gtatttcggc tggcgaatga
     8161 ccttcttcgc gattggtatt ggggcgcttg ttaccctttt gtgcctgatt aagttacttc
     8221 ccttactgcc tagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc
     8281 cggcattgat gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat
     8341 acagctatat cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg
     8401 cattactgtt attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg
     8461 gtaatcaata tgcgtctgcg ttggtaagta cggcgattgc gctgttgctg gtgtgtctgg
     8521 cactgctgct acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg
     8581 ggatcgcgat gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag
     8641 atgctaccga cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg
     8701 gtgcgttggt aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg
     8761 gcgcggtgcc tgcttttgct gcgttaatat ggtcaatcat tatatttcgc cgctggccag
     8821 tgacactcga agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac
     8881 gttttaatga tttccaggat gccgttaata ataaactgca cacccataca taccagcagg
     8941 aaccccatca ggcgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg
     9001 ccggagctgc gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc
     9061 gcaaccatca gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta
     9121 ataatcatcg ctatggttcc cggaccggcg gtacttggca ttgccagtgg tacaaaggca
     9181 atattggcac tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca
     9241 atcgctttct gttgcggaaa aagcatccga aaaccgataa acgcgacgat taagccgcct
     9301 gcaattcgca gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccagcgtaa
     9361 tacgccacca tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt
     9421 tcggcactgt tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta
     9481 gctaacggca gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt
     9541 ggttgttatc ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa
     9601 gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa
     9661 cgtggcatcg gtcaattcat tcatttgact tatacttgcc tgggcaatat taccccctac
     9721 aactaattac ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt
     9781 attccattgg gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag
     9841 tatctgtctc cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc
     9901 gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg
     9961 acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat
    10021 gataagcgcg gcgtactggt aaaacttacc accagcggcg cggaaatatg cgaacaatgc
    10081 catcaattag ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa
    10141 gttgcaacac ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac
    10201 aatgtccaga cgcaatactg acgctattac cattcatagc attttggact ggatcgagga
    10261 caacctggaa tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg
    10321 gcacctgcaa cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag
    10381 ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct
    10441 ggcagaacga tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt
    10501 tgatgttccg ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca
    10561 tccattaaat cattacaaca gctagttgat aacgtgacaa cgtcactgag gcaatcatga
    10621 aaccactttc atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg
    10681 aacaaaccac gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc
    10741 aggaacaacc accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc
    10801 tcggcgtgcc ctattataat caacacgcta tgtagtttgt tctggccccg acatctcggg
    10861 gcttattaac ttcccacctt taccgcttta cgccaccgca agccaaatac attgatatac
    10921 agcccggtca taatgagcac cgcacctaaa aattgcagac ccgttaagcg ttcatccaac
    10981 aatagtgccg cacttgccag tccgactacg ggcaccagta acgataacgg tgcaacccgc
    11041 caggtttcat agcgtcccag taacgtcccc cagatcccat aaccaacaat tgtcgccaca
    11101 aacgccagat acatcagcga caagatggtg gtcatatcaa tagtaaccag actgtgaatc
    11161 atggatgcgg aaccatcgaa aatcagcgag acaacaaaga agggaatgat tgggattaaa
    11221 gcgctccaga ttaccagcga catcaccgcc ggacgcgttg agtgcgacat gatcttttta
    11281 ttgaagatgt tgccacacgc ccaactaaat gctgcagcca gggtcaacat aaagccgagc
    11341 atcgccacat gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct
    11401 aaggcgatcc ccgccaattg tttgccatgc agtcgctccc cgaaagtaaa cgcgccaagc
    11461 acgatagtaa aaaacgcctg tgcctgtaac accagcgaag ccagtccagc aggcatacca
    11521 aagttaatgg cgcagaacag aaaggcaaac tgcgcaaaac tgatggttaa tccatacccc
    11581 aagagcaaat tcagtggtac tttcggtcgt gcaacaaaaa agatggccgg aaaagcgacc
    11641 agcataaagc gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg
    11701 accacaaaat ttagccccca tacgaccact accagtagcg ccaacacccc atcttttcgc
    11761 gacattctac cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt
    11821 ttctttaaca gttgattcgt tagtcgccgg ttacgacggc attaatgcgc aaataagtcg
    11881 ctatacttcg gatttttgcc atgcgtccga tggttaataa caacgacgag gcaagaaggg
    11941 cgctggtaga gcgtcgtagg gataagttca tttgtctgtc cggcagtgcg tttcgttatg
    12001 ttttatttta gagatgtaaa gaaatagcat ggctacgctg ccatttatga ccatttactt
    12061 gagtcgccag tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac
    12121 tattggcgtc gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg
    12181 ctatatgtta ctggcaatta ccgccttcgc cagcggtttt attgccattc ctttagtgaa
    12241 taacgtgacg ctggttgtgc tcttttttgc cctcattaac tgcgcctatt ctgtttttgc
    12301 taccgtgctg aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt
    12361 ctcaatcaac tacaccatgc taaacattgg ctggaccatc ggtccgccgc tcggcacgct
    12421 gttggtgatg cagagcatca atctgccctt ttggctggca gctatctgtt ccgcgtttcc
    12481 catgcttttc attcaaatat gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg
    12541 cagtgtctgg tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc
    12601 tggttttctg gcttcttttg taagcggcgc atttgcttca tgcatttcac aatatgtgat
    12661 ggtgattgct gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc cggtgaatgc
    12721 tgccatggtg gttacgttgc aatattccgt gggccgccga cttaacccag ctaacatccg
    12781 cgcgctgatg acagcaggca ccctctgttt cgtcatcgga ctggtcggtt ttattttttc
    12841 cggcaacaac ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat
    12901 ttatgcgccg ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag
    12961 ctatttttcc gcccagtctt taggctggct tggtgccgcg attaacccat tagtgagtgg
    13021 cgtagtgcta accagcctgc cgccttcctc gctgtttgtc atcttagcgt tggtgatcat
    13081 tgctgcgtgg gtgctgatgt taaaagggat tcgtgcaaga ccgtgggggc agcccgcgct
    13141 ttgttgattt aagtcgaaca caataaaaat ttaattcagc cttcgtttag gttatctctg
    13201 ctaatatctt tctcattgag atgaaaatta aggtaagcga ggaaacacac cacaccataa
    13261 acggaggcaa ataatgctgg gtaatatgaa tgtttttatg gccgtactgg gaataatttt
    13321 attttctggt tttctggccg cgtatttcag ccacaaatgg gatgactaat gaacggagat
    13381 aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac
    13441 ggcgaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
    13501 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
    13561 ttttctcaat g
//
LOCUS       DAECOD010000101        13549 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.101, whole genome shotgun sequence.
ACCESSION   DAECOD010000101 DAECOD010000000
VERSION     DAECOD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.101"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            34..435
                     /locus_tag="KKO15_003116"
     CDS             34..435
                     /locus_tag="KKO15_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD6361816.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="KKO15_003117"
     CDS             447..818
                     /locus_tag="KKO15_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6361817.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="KKO15_003118"
     CDS             805..1395
                     /locus_tag="KKO15_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6361818.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1793
                     /locus_tag="KKO15_003119"
     CDS             1392..1793
                     /locus_tag="KKO15_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6361819.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1804..2544
                     /locus_tag="KKO15_003120"
     CDS             1804..2544
                     /locus_tag="KKO15_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBD6361820.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2603..2989
                     /locus_tag="KKO15_003121"
     CDS             2603..2989
                     /locus_tag="KKO15_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBD6361821.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2998..3327
                     /locus_tag="KKO15_003122"
     CDS             2998..3327
                     /locus_tag="KKO15_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBD6361822.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3299..6340
                     /locus_tag="KKO15_003123"
     CDS             3299..6340
                     /locus_tag="KKO15_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD6361823.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            6340..6669
                     /locus_tag="KKO15_003124"
     CDS             6340..6669
                     /locus_tag="KKO15_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6361824.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6669..7367
                     /locus_tag="KKO15_003125"
     CDS             6669..7367
                     /locus_tag="KKO15_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD6361825.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7372..8115
                     /locus_tag="KKO15_003126"
     CDS             7372..8115
                     /locus_tag="KKO15_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6361826.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            8052..8654
                     /locus_tag="KKO15_003127"
     CDS             8052..8654
                     /locus_tag="KKO15_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD6361827.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8715..12194
                     /locus_tag="KKO15_003128"
     CDS             8715..12194
                     /locus_tag="KKO15_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBD6361828.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            12262..12861
                     /locus_tag="KKO15_003129"
     CDS             12262..12861
                     /locus_tag="KKO15_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBD6361829.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12925..>13549
                     /locus_tag="KKO15_003130"
     CDS             12925..>13549
                     /locus_tag="KKO15_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD6361830.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMA"
BASE COUNT         3192 a         3204 c         4325 g         2828 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcagg
     1501 aactgcctgc cgtggcggtg tggctgagtg atgctcagta caccggaaaa atgacggatg
     1561 aagatgactg gcaggctgtt ctgcatattg ccgtcttcat ccgggcacag gcaccggatt
     1621 cagagctgga tatgtggatg gagagcacca ttttcccggc cctgaatgat gtaccggcac
     1681 tttccggact catcgacacc ctgaacccac tcggttttaa ctatcaacgt gataatgaga
     1741 tggctacctg ggcgatggcg gaaatcacgt accagatcac gtacacgaat taaggaggtg
     1801 gtaatgacca caccaaatcc actggcaaag acgaaaggtg cggggacgac gttctggatg
     1861 tataccggca acggcgatgc gtttgcgaac cctttgtcgg acactgactg gctgcgtctt
     1921 gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga tgacacttat
     1981 ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc cgttggtgat
     2041 acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact ggttcagttg
     2101 ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac tgttgatgtt
     2161 ttccgcggct ggctgagctc actgggtaaa accattgcct caaaagacgt gatgacccgc
     2221 actgtgaaaa tcagcggtgt ggggcgtccg tatctggcag aggaaggcac tgaaaccgtg
     2281 ggtgttaccg gactgacggt ggcaccggca tctgtcagtg tcaaagcggg agcaaccacc
     2341 acactgacct ttacagtaaa acctgacggg gccagtgaca aagcgatcag tgtgcattcg
     2401 tcagatcctc agactgcttc ggtgaccctg agcgggcttg tggccacggt gaaaggcgtg
     2461 aagcagggca gtgtcagcat cgtgggcatg acctctgacg gagagtttgt ggcagtggct
     2521 gcggtgaccg tcagcgcacc ataacaggac gatactcatc attgccccgg ttatccgggg
     2581 ctttttgcat ccggagaaca tgatgtttct gaaacaggat acgtttaatt atgaaaaaca
     2641 gtccgtggtg ctcagtgagc tgtccgggct gcagagaatt gaatatctga cgtttgttca
     2701 gcagcgaacg gcaaagtttg atgcacagga gggagaactg ccggaggctg aacgacagat
     2761 tgcttttctg cgtatgggaa tggatatcaa tgcctggctg gtttcccgct cactgtggaa
     2821 tgctgagcag tctcaggatg ttgagacgct ttgcgcatcc attatgacaa catggtcgta
     2881 tgatgcgctg ggcgcggggg cggagagggt tctgtcgctg agcggtatgg ggaccattga
     2941 gaatgccggg gatgatgatc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg
     3001 cagtttgtca tgcggcttgc ccgggagttc cggcgggcag actggcggcg gatgctgtcg
     3061 gatatgtcgg ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt
     3121 gatgtgtgga tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc
     3181 ggcagcagcg atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg
     3241 gagcgaacgg acgaagaact gatgcatctt ggggaaggta tttccggagg tgtgcgttat
     3301 ggaccagata gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga
     3361 aattccccgt atcaaaaacc ttctgaatgg tgcagccagc gatgcagaac ggtcttctgc
     3421 ccgtatgcag cgttttatgg agcgtcagac tcaggcggcc cggcagacaa tgcaggcggc
     3481 ttcttcggct gcaacagcag catcagccca tgcgcagacg gtggagaaga atgcacgggc
     3541 tcatgaacgc atggcccgtg aggtggaaca aacccgtctg cgcgtggatg ccctgaatca
     3601 gaaaatgcgc gaggaacagg cgcaggccag ggcactggcg gaggcgcagg ataaagcggc
     3661 tgccgccttt tatcgccaga ttgacagtgt gaaacaggcc ggtgcggggc ttcaggaatt
     3721 acagcgtatt cagcagcaga tccgacaggc cagaaacagt ggcggggttg gtcagcagga
     3781 ttatctggcg ctgatttcgg agatcacggc gaaaacccgt gccctgacgc aggcagagga
     3841 acaggccacc cggcagaaag cggcgtttat ccgccagctt aaagagcagg caacccgcca
     3901 gaatctgtcg tcttctgagt tgcttcgcgc cagggcggct cagctggggg taagcagtgc
     3961 tgcagaagtg tatatccgca aaatggagcg ggcaggaaaa gccacacatt cgctggggct
     4021 gaaaagtgcg gcagcccggc gggagctggg ggtgttaatc agtcagatgg cgcgcggcaa
     4081 ttttggtgcg ctgaggggat ccgggataac gctggctaac cgtgccggat ggacaggcgc
     4141 actgatgtcg ccgaaaggca tgatgactgg cggcgttatt ggcggacttg tcgcggcggt
     4201 cctgggtctg ggtaaagcct ggcatgacgg ccggaaggag ggcgagaaat ttaaccgtca
     4261 gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga aactcagccg
     4321 ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg cacaggtggt
     4381 gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg ccgcacagat
     4441 ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc ggctgaagga
     4501 tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga atgccactca
     4561 gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg cccggatcgc
     4621 catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata ataatctcaa
     4681 tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct gggatgcggc
     4741 catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac aggaaaaaat
     4801 tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt acgatcagca
     4861 gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg caaaagcgca
     4921 ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg ccgcgctgaa
     4981 ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta ttaatgccat
     5041 gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg aacgttacga
     5101 aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc ggttattgct
     5161 gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca gacagtcagc
     5221 aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc tgcagcagcg
     5281 catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc tggcccgtaa
     5341 gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc tgcagaaaca
     5401 gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc tggcagacaa
     5461 agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta tgggggataa
     5521 gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc aacagttgca
     5581 acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact accggagggc
     5641 tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac gctactggca
     5701 ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt ttcagaattt
     5761 tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag tggcattcag
     5821 cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt ttaaatcctt
     5881 cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca tgatgcaggc
     5941 ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg gtgtttacca
     6001 gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt tttttgcttt
     6061 tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc cactgcgtcg
     6121 cggtgctgat ggtaagctgg gggttgtggc ggatactggt ggttcaggca tggtgatgtt
     6181 tgccccgcag tacaacattg agatcaataa cgacggcacg aacgggcaga taggtccggc
     6241 tgccctgaag gtggtttatg acctcgggaa aaaagcggcc gcggacttta tgcaacagca
     6301 ggcccgtgat ggtggccggt taagtggagc ataccggtaa tggagacgtt tcactggaaa
     6361 gtgcgcccgg atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat
     6421 ggttatgaac agcgtcgtgc ggcgggactg aataaccagt tgtcgactta cagcgtgacg
     6481 atacgtgttc gtaaaggtga acacccatct ttaaaagcct ttctggaacg gcacggtggc
     6541 gtccgcgcat ttcagtggac gccaccttat gactggaaac tgatcagggt ggtttgtcgt
     6601 aaatggtcgg caagcgtggg ggcgttatgg gtgactgtaa cggccgattt tgaacaggtg
     6661 gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt
     6721 cagcccgggt ggagctctgg gaaatcgatc tgacagaggt cggtggtgag cgttattttt
     6781 tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcgg cagtatcagg
     6841 cataccccat tcaggggtcg gggtttgaac tgaacggcag gggctgtgct gcccgtccga
     6901 cactgacggt ctctaacctg cacggcatgg tcaccgggat ggcggaagat ctgcagagtc
     6961 tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact
     7021 tcgtcaacgg aaacagcgac gccgatccgg aacaggaggt gatcagccgc tggcgcatcg
     7081 agcagtgcag cgaactgagc gcggtcagtg cctcttttgt gctggccaca ccaacggaga
     7141 cggatggcgc ggttttcccg gggcgtatca tgctggcgaa tacctgtatg tggacttacc
     7201 gttctgatga gtgtggttac acgggcaggg cagtggctga cgagttcgac aaaccaacaa
     7261 cggatatccg gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg cgcaacaata
     7321 ccggtaattt cggcggtttc ctttccatca ataaactttc tcagtaaatc catgacacag
     7381 acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgtggcttc
     7441 gtggtgagaa cgccggaggg agacaggtat cttcccagcg agaatatctc cggtgagccg
     7501 gaggaacggt tccggatggc tccggaggac tggctgcggg cacaaatgca gggtgagatt
     7561 gtggcactgg tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg
     7621 ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc ggggggcgat tcacaagttc
     7681 cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg
     7741 ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca tcgcggggat
     7801 gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat
     7861 caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg
     7921 gtgccgaatc atgccgccat ttactgcggc gatggtgagc tgctgcacca tattcctgaa
     7981 caactgagta aacgagagag gtataccgac aaatggcagc gacgcacaca ctccctctgg
     8041 cgtcaccggg aatggcacgc atctgccttt acggggattt gcaacgattt ggccgccgca
     8101 tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg
     8161 cgtttcgtca gaaactgagt gacggctggt atcaggtgcg cattgccggg cgtgatgcag
     8221 gtgaaaccga attgtctgcc cgtcttaatg agccgctggc aaatggtgca gtgatccaca
     8281 tagtaccgcg tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag
     8341 ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt
     8401 tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca
     8461 gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc tcctcactgg
     8521 ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt
     8581 cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg
     8641 tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt tttgtcgttt
     8701 atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg
     8761 acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa gggccggttg
     8821 acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc ctggatagcg
     8881 aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag caggagcaga
     8941 caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg gaagtgaaat
     9001 acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg cgcctgacct
     9061 tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg tcggaagtcc
     9121 gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac atcaccatta
     9181 agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc
     9241 cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag ctgcagaaca
     9301 aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
     9361 cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg agtcgcaatt
     9421 atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat
     9481 acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg gcctggtgtc
     9541 tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt gcggcagatg
     9601 tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg ccggacggat
     9661 ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag cgtaaggcgt
     9721 gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacgggcaga
     9781 cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac cgcagtaatg
     9841 tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc
     9901 ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg gcgacagagc
     9961 ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag atggatgcct
    10021 ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt aaaacggaac
    10081 tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc catgtaccgg
    10141 gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt ggtcgcgtac
    10201 tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccgtcct
    10261 ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc gtggaggttc
    10321 agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc gttgcagaat
    10381 acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta
    10441 tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag
    10501 aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg gtgaatggtg
    10561 tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc ggggaatatc
    10621 aggtgctggc gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc
    10681 tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacagccagg acgacggaaa
    10741 ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc cgggcggtaa
    10801 atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg gcaccggcag
    10861 cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc ccgaaactgg
    10921 ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg attgcggata
    10981 tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg atagccgcca
    11041 gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg
    11101 gcaaatcggc atttgtggag gccgtcggtc gggcgagcga tgatgcggaa ggttatctga
    11161 gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg acgcagattg
    11221 ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg aatgtcagca
    11281 atgaaatcac gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca atccagcaga
    11341 tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct gtgaagctgc
    11401 aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaatacgc
    11461 cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg
    11521 cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg
    11581 tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt
    11641 ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg
    11701 cgaactccgg gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat
    11761 tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg
    11821 actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc
    11881 agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc
    11941 ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa
    12001 tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg
    12061 ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt
    12121 atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc acagcaggta
    12181 tcagtatcag ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt
    12241 gcagaaaagc gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc cgcagccatc
    12301 tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac gctgagcgcg
    12361 gggtatcttc atgcctcgac gaacgctccc ggcagtgatg atctgaacgg gattaacgtg
    12421 aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag ctatgccaac
    12481 gctgaagatg agcaaaaaac gcattacagt gatacccgct ggcatgagga ttccgtgcgt
    12541 aaccgctggt tcagcgtgat ggcggggctg tctgtgcgcg tgaatgaatg gttcagcgcg
    12601 tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc
    12661 gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga caaccgccac
    12721 agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc
    12781 attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg tttcatcgtg
    12841 ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc ccgctggttc
    12901 aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg
    12961 gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc
    13021 gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag
    13081 gctgagccgg ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc
    13141 ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca
    13201 ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat ggtggcgcag
    13261 gcgcagcaga gtgccggggc tgcagccgga aacgcacagc agacggcgca ggatgtggcg
    13321 gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca
    13381 accgtcacag ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaat
    13441 gcagccgcag ccggacagag cgcacaggat gccgcaggtt atgcccgcgc agcagaacag
    13501 gccaaaaatg acattgatgc tgcgctgacc ggcactctga aaatggcta
//
LOCUS       DAECOD010000102        13532 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.102, whole genome shotgun sequence.
ACCESSION   DAECOD010000102 DAECOD010000000
VERSION     DAECOD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.102"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..893)
                     /locus_tag="KKO15_003131"
     CDS             complement(<1..893)
                     /locus_tag="KKO15_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6361831.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="KKO15_003132"
     CDS             1134..1934
                     /locus_tag="KKO15_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD6361832.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="KKO15_003133"
     CDS             2012..2782
                     /locus_tag="KKO15_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6361833.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="KKO15_003134"
     CDS             complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="KKO15_003134"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBD6361834.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="KKO15_003135"
     CDS             complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="KKO15_003135"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBD6361835.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5049..5771
                     /locus_tag="KKO15_003136"
     CDS             5049..5771
                     /locus_tag="KKO15_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6361836.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="KKO15_003137"
     CDS             complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="KKO15_003137"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBD6361837.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6300..7031
                     /gene="dnaQ"
                     /locus_tag="KKO15_003138"
     CDS             6300..7031
                     /gene="dnaQ"
                     /locus_tag="KKO15_003138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBD6361838.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7164..7240
                     /locus_tag="KKO15_003139"
     tRNA            7164..7240
                     /locus_tag="KKO15_003139"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7198..7200,aa:Asp,seq:gtc)
     gene            complement(7371..8417)
                     /locus_tag="KKO15_003140"
     CDS             complement(7371..8417)
                     /locus_tag="KKO15_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBD6361839.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8428..9413)
                     /gene="tssG"
                     /locus_tag="KKO15_003141"
                     /pseudo
     CDS             complement(8428..9413)
                     /gene="tssG"
                     /locus_tag="KKO15_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9410..11293)
                     /gene="tssF"
                     /locus_tag="KKO15_003142"
     CDS             complement(9410..11293)
                     /gene="tssF"
                     /locus_tag="KKO15_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBD6361840.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQLRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11309..11803)
                     /gene="tssE"
                     /locus_tag="KKO15_003143"
     CDS             complement(11309..11803)
                     /gene="tssE"
                     /locus_tag="KKO15_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBD6361841.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11800..12630)
                     /locus_tag="KKO15_003144"
                     /pseudo
     CDS             complement(11800..12630)
                     /locus_tag="KKO15_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12617..13531)
                     /gene="tagK"
                     /locus_tag="KKO15_003145"
                     /pseudo
     CDS             complement(12617..13531)
                     /gene="tagK"
                     /locus_tag="KKO15_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3102 a         3524 c         3609 g         3297 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg cgctactgcc tgcaccaatc gtcaaacttt ggcctggctt
     3061 cagcttagat ccgcgcagtt tgttccactg ctgcaaatct ttggtgctta cgccgagacg
     3121 tgaagcgatg cttgaaagcg tgtcgccaga gcgtacggtg taaacacggc tgttaagcgg
     3181 cgtattgttg gcaaccagcg tcgactgtac agcagcaatt tcgcctgaag cgagagattc
     3241 acgcagttga tctgcatgct tctttggcac catcacgtac tgcggcccac ttgcgcccag
     3301 cgtggagcct ttcacgccag cgttgaatgt cttcagcttg ctgacggaaa tccccgccat
     3361 atctgcaacc ttcgccattt caaccgggct actcaggtgc acacgcgcca gagcacggct
     3421 ttcatcggtc gttggcagac gtacgccata acgcttgctg tttttgagaa tatcactcaa
     3481 tgccagcatt ttaggcacgt actgcttcgt ttcctgcggc aacggtaacg accagaagtc
     3541 cgtggatttc ccacgcgctt tgttcgtttt aattgccttc atgacccgac cttcgccgct
     3601 gttataagcc gctacggtca gaagccagtc gtcgtcaaac attttgttca gacgctgcat
     3661 catattcagc gcggcagttg ttgaagcaac aacatcgcga cgcgcgtcat aattgcgggt
     3721 ctgtttcaaa ccataattgc gccccgtgct cggaatgatc tgccagatgc ctgcggcatt
     3781 ggcgccagac gttgcgtgag gatcaaaagc gctctccact atgggtagta gtaccagttc
     3841 cataggcatg ttacgttttt taacttgccc tgctatccag tacatatacg gctctgcccg
     3901 taaagttaca tcgtggagat agctcttatt gcgtaaatat ttctgtttct gttcgcgaat
     3961 ccggtcattt tccggaattc ccatctttag ctcgtcgcca atgaaagccc acaagtcacc
     4021 atctggcgcg atagacgtcc catcgtccat ccatcgtgcc tgacttgtaa actttgctgc
     4081 ttccccttga ccagctgcag aaaggctctg tgcgtgctgt tgaacgttgc cggtactctg
     4141 gcaacccacg agcaggacag aggcgagtaa tatcgctttt gccttcatgt gtgtgtcaat
     4201 agttgcttaa aagacgaccg atcataacgg cgaacggagc cgatgacaag aaagttttat
     4261 cagaacctat ctttctttga ccttaaccat gcaaaacgct cttcaggttg ttgcaataat
     4321 gtttcttcat taattccatt aattaaatca atatcttccg ttcttaaaaa aacattaatt
     4381 tgccgctcat ttttcagaat tacgggtagt gttatttgat tttttgcccg taactcctta
     4441 actttacgat aataatcatt tatggacaaa tcgtgcggaa aaatactcaa agcaaacttc
     4501 atatttgata acgtatattc atgagcacaa cataccaatg tatcgtcagg taacgcactt
     4561 agctttttaa gtgattgata catttgtaaa gctgtccctt caaacaaccg accacaccca
     4621 ccagaaaaca gcgtgtcgcc gcaaaataga taaggtttac tgaagtaaca gatatgtcct
     4681 aaagtgtgac ccggtgtggt aattacacta aattcatgcc ctaaaacgaa ggcagtttcg
     4741 ccatctttga ctacctgtgt tgttccctta tcttgtgtct cttgtggacc atacaccaca
     4801 atttgcggaa acttttccac cagttctttt acgccgccaa cgtgatcgtg gtgatggtgg
     4861 gttagaaata tggcctccgg ttgccagtta ttggcggtaa tggcgtttaa tactggctct
     4921 gcgtctccgg gatcgacaat caggcagcga cctgcctcat cattcaaaac ccagatgtaa
     4981 ttgtcatcaa aggcgggaat actgttaaga ttcatagatt acctctcagt gtaaaacgga
     5041 aggttgtgat gaaaccggca agagtccctc aaactgtcgt ggctcctgat tgctggggcg
     5101 atttgccctg gggagagctt tatcgcaagg cgctggagcg ccagctcaac ccgtggttca
     5161 ctaaaatgta tggttttcat ctgcttaaga ttggcaattt aagcgcagaa atcaattgcg
     5221 aagcgtgcgc ggtttctcat caagtgaatg tttctgcgca aggaatgccc gtccaggtgc
     5281 aggcggatcc acttcatctt ccttttgccg ataaatccgt tgatatttgt ctactggcac
     5341 ataccttgcc gtggtgcacc gatccgcatc gtttattacg tgaagccgat cgggtactga
     5401 ttgatgatgg ctggctggtg attagtggct tcaaccccat cagtttaatg ggattacgca
     5461 aacttgtgcc ggtattgcgc aaaacctcgc cctataatag ccggatgttt actctgatgc
     5521 ggcaactgga ctggctctct ttgttgaatt ttgaagtgct gcacgccagc cgtttccacg
     5581 ttctcccgtg gaacaaacac ggaggaaaac tattgaatgc gcatattcct gcgcttggtt
     5641 gcttacaact tattgttgcc cggaaacgga ctattccttt aacgctaaat ccgatgaaac
     5701 agagtaaaaa caagccacga attcgccagg cggttggagc cacccggcaa tgtcgtaaac
     5761 cacaggctta aacttcgact tggtagcccg tatcttccag tgtgggattc atcgccgcgg
     5821 cacgagccag ttcatcacag cgttcgtttt ccgggtgtcc ggcatggcct ttaacccatt
     5881 cccatttgat ttgatgctgc cccaatgcag catcaagacg ttgccagaga tcgacatttt
     5941 ttactggttt tttgtctgct gttttccagc cacgtttttt ccagttatgg atccactggg
     6001 tgataccctg gcggacatac tggctgtcgg tactcaaaat gacttcgcaa tgttctttta
     6061 acgcctccag cgcgacaata gcggccatca actccatacg gttgttggtg gtgcgggtgt
     6121 agcctgcgct aaaggttttc tcgcgtccgc gatagcgtaa aatagcgccg taacccccag
     6181 gtccaggatt acccagacac gaaccatcgg tgaaaatttc tacctgttta agcatctctg
     6241 gtagacttcc tgtaattgaa tcgaactgta aaacgacaag tctgacataa atgaccgcta
     6301 tgagcactgc aattacacgc cagatcgttc tcgataccga aaccaccggt atgaaccaga
     6361 ttggtgcaca ctatgaaggc cacaagatca ttgagattgg tgctgttgaa gtagtgaacc
     6421 gtcgcctgac gggcaataac ttccatgttt atctcaaacc cgatcggctg gtggatccgg
     6481 aagcctttgg cgtacatggt attgctgatg aatttttgct cgataagccc acgtttgccg
     6541 aagtagccga tgagtttatg gactatatcc gcggcgcgga gttggtgatc cataacgcag
     6601 cgttcgatat aggctttatg gactacgagt tttcgttgct taagcgcgat attccgaaga
     6661 ccaatacctt ctgtaaggtc accgatagcc ttgcggtggc gaggaaaatg tttcccggta
     6721 aacgcaacag cctcgatgcg ctatgtgccc gctacgaaat agataacagt aaacgaacgc
     6781 tgcacggggc attactcgat gcccagatcc ttgcggaagt ttatctggcg atgaccggtg
     6841 gtcaaacgtc gatggctttt gcgatggaag gagagacaca gcagcaacaa ggtgaagcaa
     6901 caattcagcg cattgtacgt caggcaagta agttacgcgt tgtttttgcg acagatgaag
     6961 agctggcagc acatgaagcc cgtctcgatc tggtgcagaa gaaaggcgga agctgcctct
     7021 ggcgggcata aatacatgta aaaggcgcta aaaatagcga cttgggcgat ttttgcagca
     7081 aacgattcaa aagatgagaa aaatcgttga cgaaggtcga ggcaatccgt aatattcgcc
     7141 tcgttcccaa cggaacacaa cgcggagcgg tagttcagtc ggttagaata cctgcctgtc
     7201 acgcaggggg tcgcgggttc gagtcccgtc cgttccgcca atattcagaa agcccgaatc
     7261 agtaatgatt cgggctttcg tcgttttggt gaagtgagaa tttttcactc cgaagtaaaa
     7321 cggatatgat ttaaaaaatg gccagacatt ataaattctg gccacttctg ttactccagc
     7381 gcatcccatt tctccagcag ttctactgga atttcattta ttacctccga gacgcgtttt
     7441 ccgaccaggc gttctgcctg gcgcagcaaa ggaatggtcg ggctgctcgg ttcgctgtgc
     7501 tcaaaccagc gacggatgac acgcaggcgt tccagcgcgt cgtttcggtc gcggatctgc
     7561 cggggttctg cgccggtcat cggtgatatt tcctccgcga tatgcaccgg tagcgcaatc
     7621 tgctctgcct gcatctgttc agtatgaacg atggtggcgt tttccggctg ctcagcctgt
     7681 ggttgtggca ggatctccgg cgtggtgctc tgcactgctc ccggtaacag ggccagcagt
     7741 tgcgtcagac gggagaagtc cggtgcctgg tcattcagcg tttcacgcgc atggcgttgc
     7801 agccgctcag tggtctctgc tgcctggcga aaggcatcca gcggcagtgt gccgcgcgct
     7861 tccaggtctg ccagttgctg gcgtaccgat tctggtgcca gcgcatccgc cgggcgtaat
     7921 gcagatagcg aacgctccac atcccgcacc tgaaggcgca tggcagcatt atttgacagt
     7981 gtaatgccac gaatatccgc cattacacct tcgtggtcca gcaacgccgc cagcgcgtta
     8041 ctgcgtgcca ccgcagcatc ttcggcggtg atatcttcag tcgcgagcag ttgaggatgg
     8101 atggcgtccg ggtacgtgga gcacaacgtt tccagcagcg tgagcgcctc cgacagtccc
     8161 ctggcaccgg cctgctggat acggcagcgc agcaggacaa ccagaatgcg gatatctttg
     8221 ctgcgcatca gcagacgatg ggcgtcccgc tcaagctccg cccagttaat gttttccggc
     8281 gtactgacaa aatcgccata ttgcgcttct tcacgcggtg cggcgcgggt gaacagcagc
     8341 aggtattccg gatcgtactc cagatccggg ccgcaggggc attccgccga aattgggtca
     8401 gacaggaata tttccattta attatcctca gtgcgccagg tactgttctg gttcgaaaat
     8461 catgcccgtc accgggttgt cgtgcatggc tttacccgcc catgcagtcc aacccagttg
     8521 gtacggacca ccggtaacag ccggaggggc ggcgtggggc tttagctgta gctcaatttc
     8581 ccagcagtat tcataaccac taaaggcgcg gaccagttct gtcaggacgg gcaggttatt
     8641 gctgccaggc agaaaacgtc ggtaatcctc cagcgtcagc gggccgataa ccagccggaa
     8701 cttatactgc atgtccggca cggcctggcc gatgagtgca ccattgccaa taacggaaga
     8761 gggggcaggc atccccagcc gggtgatctc atcgtccgcc acggctatcc agtgcaggac
     8821 atactcctgt atccggaaag gtacaccgaa atagtgcgcc agcgtggtgg ccagaccgtc
     8881 cggattgcgg gactcacgca ccagatgggc cgatgccgcc agacgagcgt gatctggcaa
     8941 cgggctgtca gcgctctccc gcaaatcctg cccgctgagg ctggcgacat aaaaggcaaa
     9001 gcggtcatgt tgcggtttat ccagaccgcc cccagcggac tgggcgctgc gccatgcctg
     9061 gtaaaactgc gtcagccagc ggtgatgaaa aagatcagaa aaacgggtca gcgtcggctt
     9121 tcagtccggt tacgcgccag ttcggtgtag tgcagcggca gtggaccgtt cggcccccac
     9181 agcccgaggc tgtacagact gatgtgcagg cgcccttcct gccagcccac actggcgatt
     9241 tctcgtggcg caaaggtcag cgcgggcgtt tgtccgaggc gaaatttttc catccgtggt
     9301 tgccaggtgt tgcccgccgg aacggtacac aactgcacat ccacgcggcg catcaggttc
     9361 agaaaaccgt aacgccaggg gggggcctgc gcctgagtga aatcgttgct catacgcttc
     9421 cccgctgtcc cgccctgaca ggccaggtca taattttgcc gcgctgcatg ctgtgcagcg
     9481 tcatctgcga gaaggtatta atggaaacat ggcgggcgat atagtgttcc agcaccaggc
     9541 cgaacagata cggactgatg ccggaaaaac cttcttcatc caccgtcagt tcacaactga
     9601 cgccacgccc atagaccagt agtccggaac cgggcagacg gcgggtgacc ggggtcgttt
     9661 tacaaccaat caggctgcgc acctggcgca attgcggact gtcatgagca ggaataaaca
     9721 ggttcagcag atctcgcaac gcctggccgc cggtacggtg atccagatcg gccagcggca
     9781 gataattaaa agataactgg cggatcaacc gccatgccat ctcacgttca gccagcgggg
     9841 gctgcggcga gcgtggcggg cggataagcc ctacgcccgc caccgggatg gctgcatcca
     9901 cggttagatc gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca
     9961 tggccgtgat ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca
    10021 gcgacaggaa cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt
    10081 ctgataagcg gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat
    10141 tattgcgggt gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc
    10201 cttccacttc ctggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca
    10261 gatgctgttc cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt
    10321 tgataaccgg ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagccagt
    10381 ccggcggcag gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta
    10441 atccggcaga caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt
    10501 catgcagcag gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg
    10561 gctccaggcc ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat
    10621 gccccggaat gccagccagg gtcgcgatgg cgctggtatg tagaagttct aacaggtgtg
    10681 aggccgtacg ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac
    10741 tgaacgttag ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt
    10801 tggccggcag gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct
    10861 cttcgatggc gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc
    10921 tttcgccttc cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg
    10981 ccagatcgcc ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg
    11041 tgacgtaatt ggggctgacc acttccagca ggcgctgggt gaaacggggg aattcagcgt
    11101 caatttttag ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctacat
    11161 acggatcggc aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg
    11221 cggcaaattc ggctcccgtt tcccgcagat agctcagttc gcggttgtag tattccagta
    11281 gccgtggatc catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat
    11341 ccagcgcgct acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa
    11401 tttcaaactg taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt
    11461 gcagagtgga cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc
    11521 ggatatcgtc ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt
    11581 taagtacgga cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga
    11641 cggtgttgag cagaaatgac aaatcccggc ggatgatctc cttaatctga accggactga
    11701 cgctgatacc gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg
    11761 tgggcagcag gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc
    11821 ggtgttaaag gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca
    11881 caccctctga cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc
    11941 ccggcacaga cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcta
    12001 ggcactgtgt gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat
    12061 cagatgcgca ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata
    12121 tgctccgccc gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca
    12181 gtcaaatgcg ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct
    12241 gcggtgagca tcagcttcct gcgcttcacc acgggcgtta caggcgaggg cggtcagcag
    12301 atcctccatc caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca
    12361 ggcgtggcgg tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata
    12421 atttgcgtcc atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc
    12481 cacgcacaat aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac
    12541 ctgttgctct gccatctgaa tgccctctac cacggaataa tcccgcagca gcgccgacaa
    12601 ggtaatcggt agctcgttat tgtttgtcat gatatatctc tccatgttgt gccgggttta
    12661 tccggtaatg gctgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta
    12721 cggtgttatg cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg
    12781 gagtcttttc gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa
    12841 tagcgtcaat gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacca
    12901 ggtcacgcaa tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag
    12961 catggcgatt aagatcttgt tcgggagata acaacgcctc gcggtattcc tgataaagtc
    13021 gggtcagggc atttcctttt tccgcacctt ctccggtgga ggaggctcca tgaacaggaa
    13081 tgatatcgaa tggattttgt gatgtcagat gctgctgcct gaaccaatcc agatccagat
    13141 attcggttat tgcttccgac gtgatggcag tctgcgatat cgtttttact gtgctcaggt
    13201 tcggagatga tggattgcca tgtacaaggc actatgaaga taacccccat tctatcaggt
    13261 cgccttcatt cagacgcata cggtgatgag gttccacaat ctggtcgttt accgtacagc
    13321 aatattcggt agactgattg ataatccacc atgcttcttc gtggcgggtc agagtgaggg
    13381 caatctttac atcatcattc accttaaaaa caggaatcca gggagaggta acggtgaaga
    13441 taatttgtgt tccttcagtc gcgtaaattg tgttatcggc gatcccgtgc gcctgtattt
    13501 tttggagtcc ccacgtaata tttggtttca tt
//
LOCUS       DAECOD010000103        13330 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.103, whole genome shotgun sequence.
ACCESSION   DAECOD010000103 DAECOD010000000
VERSION     DAECOD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.103"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            174..1673
                     /gene="pitB"
                     /locus_tag="KKO15_003146"
     CDS             174..1673
                     /gene="pitB"
                     /locus_tag="KKO15_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBD6361842.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1722..2414)
                     /locus_tag="KKO15_003147"
     CDS             complement(1722..2414)
                     /locus_tag="KKO15_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD6361843.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2588..3301
                     /locus_tag="KKO15_003148"
     CDS             2588..3301
                     /locus_tag="KKO15_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD6361844.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3333..4091
                     /locus_tag="KKO15_003149"
     CDS             3333..4091
                     /locus_tag="KKO15_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361845.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4137..5486
                     /locus_tag="KKO15_003150"
     CDS             4137..5486
                     /locus_tag="KKO15_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBD6361846.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5486..6310
                     /locus_tag="KKO15_003151"
     CDS             5486..6310
                     /locus_tag="KKO15_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361847.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6322..6882
                     /locus_tag="KKO15_003152"
     CDS             6322..6882
                     /locus_tag="KKO15_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD6361848.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6913..7983
                     /gene="lptF"
                     /locus_tag="KKO15_003153"
     CDS             6913..7983
                     /gene="lptF"
                     /locus_tag="KKO15_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD6361849.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7980..9059
                     /gene="lptG"
                     /locus_tag="KKO15_003154"
     CDS             7980..9059
                     /gene="lptG"
                     /locus_tag="KKO15_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD6361850.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9095..10266)
                     /locus_tag="KKO15_003155"
                     /pseudo
     CDS             complement(9095..10266)
                     /locus_tag="KKO15_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10266..10514)
                     /locus_tag="KKO15_003156"
     CDS             complement(10266..10514)
                     /locus_tag="KKO15_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD6361851.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10547..11461)
                     /locus_tag="KKO15_003157"
     CDS             complement(10547..11461)
                     /locus_tag="KKO15_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBD6361852.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11458..13188)
                     /locus_tag="KKO15_003158"
     CDS             complement(11458..13188)
                     /locus_tag="KKO15_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBD6361853.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            complement(13246..>13330)
                     /locus_tag="KKO15_003159"
     CDS             complement(13246..>13330)
                     /locus_tag="KKO15_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6361854.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         3198 a         3264 c         3461 g         3407 t
ORIGIN      
        1 gtggaatacc tgtgttttga ttatttctaa aggttttgaa tgaatgctta ttgtctgata
       61 cacaagaaat aacactcttt ttatcgttaa aaaatgatat ttcactttgc ccatgccgtt
      121 aaagtcactg ataatgcgtc cgttcgtaaa ttcaaaatgg cgtaatctaa tatatgctaa
      181 atttatttgt tggccttgat atatatacgg ggcttttgtt attacttgct ctggcatttg
      241 tgttgttcta cgaagcaatc aatggttttc atgacacggc gaatgcggtg gcaaccgtta
      301 tttatactcg tgccatgcag ccacaacttg ctgtggtgat ggcggcattt tttaactttt
      361 ttggcgtgtt attgggcgga cttagcgttg cctatgccat tgtccatatg ttaccaatcg
      421 atttgttgct gaatatgggg tcaactcacg gtttggcgat ggtcttttcc atgctgctgg
      481 cggcgattat ctggaacctg ggaacgtggt tctttggttt accggcctcc agttcgcaca
      541 ccttgattgg tgcgattatc ggcatcggtt taaccaacgc gctgttaacc ggctcatcgg
      601 tgatggatgc gttaaacctg cgtgaagtaa ccaaaatttt ctcctcgctg attgtttccc
      661 ctatcgtcgg cctggtcatt gcgggtggtc tgattttcct gctgcgccgt ttctggagtg
      721 ggacgaaaaa gcgtgaccgt attcaccgca ttccggaaga tcgcaaaaag aaaaagggca
      781 aacgtaaacc gccattctgg acgcgtattg cgctgattgt ttccgctgcg ggcgtggcgt
      841 tttcgcacgg cgcgaacgac gggcagaaag ggatcggcct ggtaatgctg gtactggtgg
      901 ggattgcccc tgctggcttc gtcgtcaata tgaatgcgtc cggctatgaa attacccgta
      961 cccgcgatgc cgttaccaac ttcgaacact acctgcaaca gcatcctgaa ctgccgcaga
     1021 agttgattgc gatggaacct ccattgcctg cagcatcgac tgatggcacg caagtaacag
     1081 agtttcactg tcatccggca aatacctttg atgcgattgc gcgcgttaaa acgatgctgc
     1141 caggcaatat ggaaagttac gagccgttaa gcgtgagtca gcgcagccag ctgcgccgca
     1201 ttatgctgtg catctccgat acctccgcga agctggcgaa actgccaggc gtcagtaaag
     1261 aagaccagaa cctgctgaaa aaactgcgca gcgatatgtt aagcaccatt gagtacgctc
     1321 cggtgtggat catcatggcg gtagcactgg cgctcggcat tggcaccatg attggctggc
     1381 gtcgtgtagc gatgaccatc ggtgagaaga ttggtaagcg cggcatgacg tatgcgcaag
     1441 gcatggcggc acaaatgacg gcggcagtgt ctatcggcct tgccagttat attgggatgc
     1501 ctgtctccac aacacacgtc ctctcgtctg cggtagcagg gacgatggtg gtggacggcg
     1561 gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc gtgggtgttt actttaccgg
     1621 cggcaatttt tctttctggt gggctgtact ggatagcatt gcagttgatt taaccttcct
     1681 gaaaatgccc ggtcctggcg atcgggcatt tccatttttg actagtgata accacgcgcg
     1741 gtcataaaat ccgtaatcgc tttttctgca tcaaccaaca cctgttccag tggctgattg
     1801 gcatcgatat caaccagttg tgcaccacca aaggttaact gtggcgttat ggcaatcttc
     1861 ctcgccagcg attcccgttt atggtcgggt ttacgtgcac aggcaacttc aaggtcaaca
     1921 ttgagtttga tgaccagatc aggcttatgg ctcgccatcc agtgaaacgc tttacgttcc
     1981 tggcttgcca gccatgagac aaaacgacca ccttcaacgt taggtgggaa caccgtacca
     2041 tcgtaagcgc caggaatttg gtcctgagga taacggtcgg ttagaacaat taacccgcga
     2101 cgacgacagg caagcatatg acgaaagcgc agtaagcgac gggcgacaaa cgctgtaatt
     2161 accagcgccg gaactggtcc aggcaatttt tttgctgttt tcacctgatt tcgttcaatt
     2221 gttttatgta aggattttcc catcaaaggt aattttgtca ctgcacgacc gacatttccg
     2281 gcctgttttc ctaaatgaac tctttcggca gcaccatatt tttcgacaac ggtaataaga
     2341 tgttcacaca ccgttgactt gcctgaacca tcgctaccaa taacggcaat taatggaggt
     2401 gttattgact gcatgaatat atcctttaat ttaaatatcc attaaaaata tttatttggt
     2461 taatatgttt ttatgaaagc gtaattcagg tcaatgtcat aattagccat tgtcacaata
     2521 aggttgaacg gatattctga gcacggacca cgtaatattc aatacattat ttactgccgg
     2581 gtttttcgtg aatcaacgta atatgtcaat aattaattcc acgccagtgc gtgttattgc
     2641 cattgtagga tgtgatggtt caggtaaatc gaccctcacg gcaagcctgg taaatgaact
     2701 ggcagcaaga atgccaacag aacacattta tctcgggcaa tcgtccgggc gaattggcga
     2761 atggatttca cagctccctg ttattggcgc accttgtggg tgttatctgc gaagtaaagc
     2821 ggcacatgta cacgaaaagc ccttaactcc gcctggcaat attactgcgc tggttatcta
     2881 tctgctttcc tgctggcggg cgtacaagtt tcgcaaaatg ttgtgtaaaa gccagcaagg
     2941 ctatctgctc atcaccgacc gctacccgca ggttgaagtg ccgggttttc gctttgatgg
     3001 cccgcaattg gcaaaaacca cgggcggtaa cggttggata aaaatgttaa ggcagcgcga
     3061 gttgaagctg taccaatgga tggcgtctta tttacccgta ttgttgattc gtcttggcat
     3121 tgatgaacaa accgcgtttg cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa
     3181 aatcgccgtt acgccgcaac tgacattcaa tggcgccaag atccttgaac tggatggtcg
     3241 gcaaccagcc gatgaaatta tgcaagcgtc actacgcgca attcacgccg cgctttcctg
     3301 atcaccaaaa tggatagggg atgatttaac gaatggatgc actacaaaca caaactgtta
     3361 atagtacaac cgcgccgcag cccaactaca ttccggggct gattgcggtg gtcgggtgtg
     3421 atggcaccgg taaatccacg ctgaccaccg acctggtgaa atcgctgcaa caacactggc
     3481 aaaccgagcg ccgctatctg gggctgctct ccggcgaaga cggcgacaaa atcaaacgat
     3541 tgccgttggt tggcgtctgg ctggaacggc gattggcggc caaatcctcg aaaacccaaa
     3601 gcatgaaaac caaatctccg gcgctatggg cggcggtgat tatgtactgc ttctcgctgc
     3661 gaagaatggc gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca
     3721 gcgatcgctt cccgcaggct gaaatttcgg gcttttatta tgacggaccg gggatcggcg
     3781 tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg agtttatacc
     3841 aaaaaatggc gcaataccgt cctgaattaa tcattcgttt ggggattgat attgatactg
     3901 ccatctcccg caagcctgac catgagtatg ccgagctgca ggacaaaatc ggcgtcatgt
     3961 cgacgattgg ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg
     4021 aagtgctgga acaggtacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa
     4081 gtttaactta aggctggcgg gatatttttt actatcaacg ataaggtcag gctgaattgg
     4141 cgggttttaa catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata
     4201 gcgcctggtt aggctccagt aatgtcgaga gcgccttgct ggggctgttg gcgctctcgt
     4261 gtgccggtaa agggatgacg cccgccatgt ttggtgtact ggtgattgtg caatcgtacg
     4321 ccaagtcgat cagcgatttt attaagtttc agacatggca gctggtggtt cagtacggaa
     4381 cgccagcctt gaccaacaat aatccgcagc aatttcgcaa tgtcgtctca ttttccttct
     4441 cgctggatat cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctgccattcc
     4501 tttcccattc attaggtctg gatgaccaaa gcttttggct ggcggcgctg tattgcacgc
     4561 tcattccttc aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgctttg
     4621 atttgattgc tgtacagcag gcgacgaaac cttttctgcg cgcagcgggg agcgtcgcag
     4681 cctggtattt tgactttggt tttgcgggtt ttgttattgc ctggtacgtg tcgaatctgg
     4741 ttggcggcac catgtactgg tggtttgccg cgcgcgaatt acgccgtaga aatatccata
     4801 acgccttcaa attgaatctg tttgagtctg cccgacacat taaaggcgcg tggagttttg
     4861 tctggtcaac caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgt
     4921 tggtggggat cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct
     4981 tcgacgccgc cggaacgcca gcgggtttgc tgggtaaaag tttttaccca gaggtgatgc
     5041 gtttagatcc gcgcaccacc agaccgtggt tgttaggtgt gaagtccggt ttactggcgg
     5101 gcggaatcgg tattctggtg gcgctcgcgg tgttgatcgt cggcaagccg ctcatttcgc
     5161 tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa
     5221 ttgtgatttc gatgctgggc ttcccgcagg agtcattgct attgatggcg ggaaaacagc
     5281 gcgcttttct cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt
     5341 tctgtcatct gtttggtgtg ctgggagcag cgtttgctta cttcggtggg caatgtctgg
     5401 acgtggcgct ctcgttaatt ccaaccttaa aggcattttt tcagcgccat tccttgcttt
     5461 ataacgcagc cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga
     5521 gcgtctattt gttgcgattg aagaagatga tgtcgttgat gcacaggtta gtttgccgcc
     5581 gactattgca ctaagttgta ccaccgaaat cattcatgat aattacgcgt tatgcctgca
     5641 attctggctc aatggcgttg atcgtcagga attactgcgt ttagttcgca aacaagccaa
     5701 aggcgatgag ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa
     5761 gcacctgcgt tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg
     5821 caaaacgacc gtttttctgg ggcgttttca ggacggcttt cgtaacggta agaaaaatat
     5881 tgtgtcgtat tacggcaacc tgttacggat ttatttaagc tctccggtct ggtcgttggt
     5941 gaactattca ttacgccata gccagttaga aagcgtaagc agttttattg cctatcggca
     6001 aaagcaaatg cataccctga aagagattat tgccaaaccg cgactaaccg gcagggaatt
     6061 tcatgatgtg cgaaaaatca tcagccagca ggtttcttat tacgacacct taagatcgct
     6121 tgatcctgaa aataaagagg cgttacagat ttcccgtttc ctcgccgcca ttaatggcct
     6181 gatgggagat aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatta
     6241 tgcaccgttg gcgctggata gcgatatttg tcagcgctta gagctgctga tttcgcgttt
     6301 tccgctatag gcggagcaat gatgtcatct cgtttgatta ttgcgttgat tatcatgctc
     6361 ttagccccgg gtgtgcaggc acacaatttc gtcactggca aaacggttac ccctgtttat
     6421 atccaggaag gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg
     6481 aacagcacac aattagcggg aaaagtccgc attattcagt atatcgccgg gcgtaagtcg
     6541 gcgaagaaaa agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat
     6601 cgttttcagc ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt
     6661 gttgttggca aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cgttattgac
     6721 ggcaatggac agggacgcgt tgcctggcgc ttgccggagc agagttcaac cattctggtg
     6781 ctgaataaag ccgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta
     6841 gatcatgtca ttgcgttggc gcagaaactg atcaatgaat aaaaaactca aaggattgag
     6901 ggcgttagtg agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg
     6961 ctgattatcg tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg
     7021 accgaagcgg caaatgggac attagctctt gatgttgtgc tggatatcgt tttctacaaa
     7081 gtactgattg cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg
     7141 ctagggcaga tgtataccga ctcggaaatt accgctatct ctgcggcggg gggcagtccg
     7201 ggacgcttgt acaaagccgt tctttatctg gcgataccgc taagtatttt tgtcaccctt
     7261 ctgtcgatgt atggtcgtcc gtgggcttat gcgcagattt atcaactgga gcagcagtcg
     7321 cagtcggagc tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga
     7381 cgaatgatcc tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt
     7441 tatacttcta ctgccaatcg aacccgtatt ttccgcgccg gttcggttga tgtggttgac
     7501 ccatcacccg agaaaccgac cgttatgttg cacaacggca ctgcctatct tctcgatcat
     7561 caggggcgtg acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg
     7621 gatcaaagcc cgaacgtcaa acgcaaagcg aaatcggtca cggagctggc gcgctccgcc
     7681 tttcctgccg atcatgccga actgcaatgg cgacaaagcc gtggcctgac cgcattgttg
     7741 atggcgctgc tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaacg
     7801 ttattgccac tgacgttgct gtttattgcc attttttatg gcggcgacgt ctgccgtaca
     7861 ctggtggcta acggcgcgat tcccctcatt cctggtttgt ggttagtgcc cggactcatg
     7921 ctaatgggcc tgctgatact ggtcgcacgt gacttctctt tgctgcagaa attttcccga
     7981 tgaatgtttt cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgctgcag
     8041 ggctattgct gccgcttttt accaccttca acctgattaa cgaactggat ggtgtcagcc
     8101 cgggcggtta tcgctggact caggcggtgc tggtggtgct aatgacctta ccgcgcacac
     8161 tggtcgaact ttcgccgttt atcgcgttat tgggtggtat tgttggactg gggcagttat
     8221 cgaaaaacag tgagcttacc gccattcgca gcacggggtt ttctatcttc cgtattgcac
     8281 tggtggcgct ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg
     8341 tggcgtcgcc attgcagcaa caggcgttgc aaatcaaatc gaccgccacc gcgttggggg
     8401 aggacgatga cattaccggc aatatgctgt gggccaggcg tggtaatgaa tttgtgacgg
     8461 tgaaatcgct gaacgagcag ggccagccgg tgggcgtgga gatatttcat tatcgcgacg
     8521 atctctcgct tgaatcctac atttatgcac gcagtgccac cattgaagac gacaaaacgt
     8581 gggtcctgca tggtgtgaat cataaaaaat ggctgaatgg caaagaaacg ctggaaacat
     8641 tagataatct tgcctggcaa tcggccttca ccagtatgaa tcttgaagag ttatcaatgc
     8701 cggggaatac tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg
     8761 ggcaacccag cagcgaatac cgccttgcac tgtgggaaaa actggggcaa cccatcctga
     8821 ccctggcgat gattttgctg gctgtgccgt ttacctttag tgccccgcgt tcgcctggga
     8881 tgggtagccg tctcgctgta ggcgtcatcg tgggcttact cacctggatc agctatcaaa
     8941 tcatggttaa tctgggattg ctatttgcgt taagcgcacc tgttaccgcg ctcggtttac
     9001 cggtagcgtt tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa
     9061 cccgccgtgg cgcaaacacc acggcgggct cacattactg cttcagagta gcaaatgcct
     9121 gaatgatggc atcgatttgc tcacgactgt gggcagcatt tacgctacag cgcagcagcg
     9181 tgatgcctgc aggcgcggca ggcggcagga taagattcac atacacgccc ggcgagataa
     9241 gctcgcgcca gatgcgtaaa ccttcctctt tcgaaccgat tattactggc actaccgggc
     9301 taatatgtga acttaactca taacccagtt tttgcagacc gtcatacaga tggttggcgt
     9361 tatccatcag cttttgccgc agctctggtt gtgtggcaat agttcttaac gacgagcgca
     9421 cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat atacgggcgg ctgccgtagc
     9481 gcagcacttc catcgcttcc gaaccgacag caaaaccgcc aatcgaggcc agacttttac
     9541 tgaaagtacc aacgataata tccacatcat cttcaacgcc gacagcttca gccagtccgc
     9601 gccccgttgc gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga
     9661 gacgacgttt gatatcaaca atttccgcca gcggcgcaac gtcaccgagc atgctgtaga
     9721 tgccctcaac gatgataatc gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt
     9781 ccagatcttt agcatcgtta tggcggaaac gaataatttc cgcgccaccg agagaacagg
     9841 cgtcataaat actggcgtgg ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa
     9901 gtgcgctgat gacgcctaaa ttagcggtat aaccggttga aaaacgatag cggtagggag
     9961 attgaaaaac tcggcaatct ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc
    10021 catacgagaa ccggtggtgc cggtgccttg ggccgctaac gcggcctgac cttcgctgat
    10081 ggcatcatga ttaaaggtta aaccgagata attattggtc ccggccagaa tcactttttt
    10141 attgccgatc cggccttcgg tggcggaata cacttcatca atacaggtgc caaacggtgt
    10201 taacccgttg tcactaaatt gcagacgctc gccagcaagg cgcgcatatt tatcgtatag
    10261 ccccattatt tattctccag ccatggaagt agagtttcca ttaattgcgc aggggttttg
    10321 acatccagca gaatattaat aggaatggaa atatcgaatc tgtcttccag catcattaag
    10381 agatccatca ctttgatcga ctccaggcca agatcgttga cgagatcgct gtcgggctta
    10441 atttccacac cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc cattactatt
    10501 tcacgattta ccatagaaac gttaacacac ctttaaaaca agacaatcaa aatagcccgt
    10561 tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt gatatcttca gaaatacggt
    10621 tattacttgc cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg gtcagcatag
    10681 gttctttacc tgccaggcga ctcaacgcgg tactgatatc cgcaaggcct gtgagtaccg
    10741 gcagaggaat gtcaaccatt cgcacggaac cacaacgggc tttggcggca agctgtcgta
    10801 cgcgttgcca gtcatagcca ccggcgacgc catcgcataa ttcataggtt tgcgtctgta
    10861 cagtttcggc gcttaaccac tgacccactg cttgcgcaaa atcggtgacg tgaagaaaag
    10921 agagttgtgt atctggcgca ccaagtcgtg gcagcaggcc gcgtagcatc cagtcaaaca
    10981 gcggttttaa ctctttatcg ccgggaccat acacggctgt cgggcgaaaa acgcccagcg
    11041 taatttcgtc cgccattgct gccagtcgtt gctcggcgac gtatttggat tttgcatacc
    11101 aggagagctc gggatggcgc gccgccagcg aagagataaa cagaaaacgt tggcaaaagc
    11161 cgctctcttt tgctgcttgc atcaggcgca ggctgccgtc aacgttacag cgggtgaaaa
    11221 tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac cactacgctg gctccggcaa
    11281 ccagctcgct aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg agattatcat
    11341 tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgcgg gcgagcaggt
    11401 tatcgataat atatttaccg ataaacccgg tagcgcccgt caccgcgaca gtttgattta
    11461 tgccagggat tcctgcacat taagactggc agcataagcc ttctgataac gttttttcgc
    11521 ttccgcacgg gcaggcttgc cggaggacgt tcggggaata ctgtggggcg gtaacagatc
    11581 gatagccgcg gtcacgccaa attcgctttg gatccgtgcc gccagcgcgt ggataagctg
    11641 cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt tttcctgggc
    11701 ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt tcctgttccg ctatatattc
    11761 aatatcctgc ggccagatat tacggccacg aataataatc agatctttaa tgcgtccggt
    11821 gacatacaga taaccgtcca gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc
    11881 ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcagac tgggaccgga
    11941 gatgcaaata tggcctacca cacgttccgc gaccggcata cctgcttcat tgcggatttc
    12001 aataccatgt tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg cgcgtgtctc
    12061 tgcacccggc gcgacggctt taccctgata ttcgaggatg tcgcgatcca cttcgttaac
    12121 cacaaccccg gaggcttcat cagagaagct gacagccagc gcattttctg ccagtccgta
    12181 gcacggcatg aaagttttat tgtcaaagtt aacctgacgg aaacattcag cgaattgatg
    12241 gagttgttct gcggagatcg gctcagcacc aataccagcg acgcgccagc aggaaagatc
    12301 cagttcagcg agatcttttt cattcacgcg gcgttggcac aattcatagc caaacggcgg
    12361 cgcaacggaa acggtgccgc gatttttact gatcaattta agccattgca aaggacgcat
    12421 ggcaaaatcc tgagtgcgca aataatctac tgaaagttgc gtggcgacgg ggggcaggag
    12481 aaagccgacc agtcccatat catggtagaa aggctgccag gagacgcagc ggtcgctagg
    12541 gcgtaattta atgccatcgt ggcttatagc acgtagatta gccatcactt cgcgatgggt
    12601 gataatgacg ccacggggaa aacgggtgct gccggaggtg tactggaggt aggcgatatc
    12661 gttcggaact ggacgctgga gcgcaacatc ggcttccggt aatgccttaa accaagcgtg
    12721 gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg gcaaccactc
    12781 atcaccagtg ataatggctg cgggctggca acttgccagt aaaccctgga gtttggcgct
    12841 ccaggaatcc cgctgaccga cgcccattgg aatcgccaac gggacggcga ctaagccagc
    12901 atactgacag gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa tcagcgccac
    12961 gcgttcgcct tttttcaggt tcagcgataa caaccgcttc gcaccagctt cggcacgcgt
    13021 ttttaatgtc tgatattcca gttgatcttc aagttggcaa cgtctgtcat aaaaattcat
    13081 tccggctcta ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa aatcggcata
    13141 gcgcataggt agggaatgcg taaagatttt attagacata tacaccacga aaaaattgga
    13201 tatcaaaaat aaaatgacaa taatgcggtg ggtaatgact ccaacttact gatagtgttt
    13261 tatgttcaga taatgcccga tgaccttgtc atgcagctcc accgattttg agaacgacag
    13321 tgacttccgt
//
LOCUS       DAECOD010000104        13327 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.104, whole genome shotgun sequence.
ACCESSION   DAECOD010000104 DAECOD010000000
VERSION     DAECOD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.104"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..246)
                     /locus_tag="KKO15_003160"
     CDS             complement(<1..246)
                     /locus_tag="KKO15_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBD6361855.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMF"
     gene            565..1146
                     /gene="mdaB"
                     /locus_tag="KKO15_003161"
     CDS             565..1146
                     /gene="mdaB"
                     /locus_tag="KKO15_003161"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBD6361856.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1177..1491
                     /locus_tag="KKO15_003162"
     CDS             1177..1491
                     /locus_tag="KKO15_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD6361857.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1539..3431)
                     /gene="parE"
                     /locus_tag="KKO15_003163"
     CDS             complement(1539..3431)
                     /gene="parE"
                     /locus_tag="KKO15_003163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBD6361858.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKSVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3460..4041)
                     /gene="yqiA"
                     /locus_tag="KKO15_003164"
     CDS             complement(3460..4041)
                     /gene="yqiA"
                     /locus_tag="KKO15_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBD6361859.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4041..4868)
                     /gene="cpdA"
                     /locus_tag="KKO15_003165"
     CDS             complement(4041..4868)
                     /gene="cpdA"
                     /locus_tag="KKO15_003165"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBD6361860.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4893..5315)
                     /locus_tag="KKO15_003166"
     CDS             complement(4893..5315)
                     /locus_tag="KKO15_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBD6361861.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5316..5945)
                     /gene="nudF"
                     /locus_tag="KKO15_003167"
     CDS             complement(5316..5945)
                     /gene="nudF"
                     /locus_tag="KKO15_003167"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBD6361862.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6150..7631
                     /gene="tolC"
                     /locus_tag="KKO15_003168"
     CDS             6150..7631
                     /gene="tolC"
                     /locus_tag="KKO15_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBD6361863.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALSTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7779..8450
                     /locus_tag="KKO15_003169"
     CDS             7779..8450
                     /locus_tag="KKO15_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBD6361864.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8456..9616
                     /locus_tag="KKO15_003170"
     CDS             8456..9616
                     /locus_tag="KKO15_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD6361865.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9654..10442)
                     /gene="ygiD"
                     /locus_tag="KKO15_003171"
     CDS             complement(9654..10442)
                     /gene="ygiD"
                     /locus_tag="KKO15_003171"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBD6361866.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10585..11346
                     /gene="zupT"
                     /locus_tag="KKO15_003172"
     CDS             10585..11346
                     /gene="zupT"
                     /locus_tag="KKO15_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBD6361867.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11404..11574)
                     /locus_tag="KKO15_003173"
     CDS             complement(11404..11574)
                     /locus_tag="KKO15_003173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361868.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11835..12488)
                     /gene="ribB"
                     /locus_tag="KKO15_003174"
     CDS             complement(11835..12488)
                     /gene="ribB"
                     /locus_tag="KKO15_003174"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBD6361869.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12592..12740)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12862..13152
                     /gene="ubiK"
                     /locus_tag="KKO15_003175"
     CDS             12862..13152
                     /gene="ubiK"
                     /locus_tag="KKO15_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBD6361870.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3418 a         3531 c         3206 g         3172 t
ORIGIN      
        1 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa
       61 aaatgctgca atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca
      121 aatattgcct cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagaatctat
      181 taaccaataa gtaagcatta tatatagcaa tgcttcaaat attgttttaa ttttctgctt
      241 taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata
      301 ttttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg
      361 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
      421 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
      481 aaagactttg cacattttgc taatttcaca gtaccgctct gtgacgtact atagtcgaca
      541 aacgtctcac cttgagatta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
      601 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
      661 cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg
      721 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
      781 ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcacgggact
      841 ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc
      901 ctggtacagg gcaaaaaata tatgctttct ctaacctgga acgcaccaat ggaagccttc
      961 accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat
     1021 aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata
     1081 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
     1141 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
     1201 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
     1261 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct
     1321 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
     1381 atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta
     1441 aaaggcgacg tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt
     1501 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
     1561 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
     1621 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
     1681 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
     1741 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
     1801 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
     1861 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
     1921 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
     1981 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
     2041 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
     2101 atcttacctt tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat
     2161 ccgcctgcgg agtcaccttc cacgaggaac agttcggtac ggttaaggcc ctgcgcggta
     2221 caatcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt
     2281 ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc
     2341 gcctgaacgt tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat
     2401 gccgcacact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
     2461 attttaaccg acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg
     2521 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
     2581 cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg
     2641 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
     2701 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
     2761 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
     2821 tctttaaagg taatctcaac gccggggcac agtactgatt tggccttcag cacatgcgtc
     2881 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
     2941 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
     3001 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
     3061 ttcgacaagg cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
     3121 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
     3181 actccttctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac
     3241 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
     3301 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
     3361 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
     3421 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
     3481 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
     3541 tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc
     3601 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
     3661 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
     3721 ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac
     3781 cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga
     3841 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc
     3901 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
     3961 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
     4021 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
     4081 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
     4141 cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
     4201 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
     4261 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
     4321 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
     4381 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
     4441 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
     4501 tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg
     4561 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
     4621 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
     4681 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
     4741 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
     4801 gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag ccagaggaag ggttaacagg
     4861 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
     4921 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
     4981 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
     5041 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
     5101 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
     5161 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
     5221 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
     5281 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
     5341 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
     5401 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
     5461 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
     5521 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
     5581 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
     5641 ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac
     5701 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
     5761 aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg
     5821 ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
     5881 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
     5941 agcattgtta atgtcctagc actaatagtg aattaaatgt gaatttcagc gacgtttgac
     6001 tgccgtttga gcagtcatat gttaaattga ggcacattaa cgccctatgg cacgtaacgc
     6061 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
     6121 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
     6181 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
     6241 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
     6301 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
     6361 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc
     6421 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
     6481 aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
     6541 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
     6601 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
     6661 tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
     6721 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
     6781 actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
     6841 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
     6901 gccaggacct ggagcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg
     6961 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
     7021 ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct
     7081 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
     7141 ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt
     7201 cctccttcaa caacattaat gcttctatca gtagtattaa cgcctacaaa caagccgtag
     7261 tttccgcgca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
     7321 ttgttgatgt gttggatgcg accaccacgc tgtacaacgc caagcaagag ctggcgaatg
     7381 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgagt acgttgaacg
     7441 agcaggattt gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
     7501 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
     7561 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
     7621 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
     7681 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
     7741 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
     7801 gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg
     7861 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
     7921 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
     7981 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
     8041 gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc agcccaggct ggcatggcac
     8101 cagaaaacca ggcgcaggct cagcaatcca gcgggagttt ctggatgccg ctgatggccg
     8161 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
     8221 cctcgaaaaa cccggccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
     8281 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac
     8341 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
     8401 ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga
     8461 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
     8521 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
     8581 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
     8641 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac
     8701 ctggagtttt gtgcgtcaat catggctgac tcaccagcca tcgctttatt cgcgtcttga
     8761 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
     8821 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
     8881 taacttgtcg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
     8941 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
     9001 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc
     9061 caccgagttc ctctacatcg acgagatagg tttaggcgaa aaaggccagt tcacggattt
     9121 acaggatcag gtgatttcta acctgttcaa gctttatccg tgggaattta tgctgcgcga
     9181 aatgttctcc accaagctgg aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag
     9241 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
     9301 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
     9361 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga
     9421 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
     9481 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg
     9541 aattggcatt cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca
     9601 tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct
     9661 atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt
     9721 ggctcctgcc catcccacac acctaacaca tacaacaacg gcagatagtg ctctggcgtt
     9781 gggttcgata acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc
     9841 ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac
     9901 ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg
     9961 accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag
    10021 gcggcaggtt tgctactgtc gatactcaac tgcaccatag ggatatcggc gtcaggatac
    10081 atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc
    10141 accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca
    10201 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    10261 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    10321 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    10381 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    10441 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    10501 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    10561 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    10621 gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc gaaccgctta
    10681 ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    10741 cttcctgccg cactagcagc tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc
    10801 ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca tccgcaggat
    10861 ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca agcgcacagc cattctgctc
    10921 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    10981 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa
    11041 ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg
    11101 tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    11161 ggtagcatga tttccccggt ggtcatggcg gcggttgcag gaattatggt ggcgctctcg
    11221 gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc cagctatggc
    11281 gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac ggcgggaatt
    11341 ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag cttcacgaaa
    11401 gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc
    11461 ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa
    11521 ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa acatcctttt
    11581 acccttaata tttaacaata ctaaacattt agcgcataaa tttcacatat cctttttcgg
    11641 atatattcat atggtcgcat aataacggaa caattatatg attttgatca taatgaaaaa
    11701 gaatgcctga tattaatgaa attttctcat agaaagagaa gcggaattaa catcttgcat
    11761 agcagggcaa aataaaaacc gaagccagct acaacttcgg tttcttgatt aaggcagtaa
    11821 ttaagcagcg gttttcagct ggctttacgc tcatgtgcct gacggtatgc caccaggtct
    11881 tcaatagtca cgagcgccat attgtgttta ttggcaaact caatgcactc tggtgcacgc
    11941 gccatcgtgc catcgtcatt agtcagctca cacagcacac cagccggttt aaagcctgcc
    12001 agcgtcatca aatcaatagt cgcttcagta tgaccgccac gcgtcagtac accacctgcc
    12061 tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat cagaaggttt tgcgccatcg
    12121 gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag aaacaccggt agtcacacct
    12181 tcagccgctt caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt attttctacc
    12241 atcattggca gatcaagttg tttacggcga tcttcagtga tgcacaggca aacaataccg
    12301 ctaccgtggc gaatggtcag cgccatctgc tcaacggtca tggtttctgc cgggaagatc
    12361 atatcgcctt cgttttcacg gtcttcatca tcaagcacca ttacaccgcg tccttcacgc
    12421 agcgcagcca gtgcattttc aacacgttcg aaaggcgtac caaaagagga aagtagcgtc
    12481 tgattcatgg taaaaaaacc tcactaaaat tatggttacc agaatcaggg cagtcttagg
    12541 agtggcggca tatagccaaa ataacgtgag cgggtccatg cccgacagaa tcgttactct
    12601 ctcccatccg gactctaacc gtcggccccg gaattacacc ggatctgctg tcctttgagt
    12661 tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt taccgccggt ggggaatttc
    12721 gccccgccct gagaataagc ggattcacta taacgctaat gattagcggc agcaacgcat
    12781 agcttcacat aattctggtt tatgacttac ccttatcgca ctacaatggt actcaacacc
    12841 tatcagtaca gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag
    12901 gttcacgaat caatgcctaa aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc
    12961 cgccaaaccc tacaagctca gctgacgcgc ctcgaccttg taagccgtga agagttcgac
    13021 gtccaaacgc aagtcctgtt acggactcgt gagaaactgg cgctgcttga acaaagaatc
    13081 agcgaactgg aaaatcgcag tactgagatc aaaaagcagc cagatccaga gactctcccc
    13141 ccaacgctgt aaacatttgt ctttaatgca ctgtaaatta tgaattaatc ctctaattta
    13201 cagtgttttc tattacatta tcgcttaatc ccattcagag tatcatcaca aaaatgaatg
    13261 attgcaattg aaaatataat tgttatgtat tctttaagtt ttacttaatc gatatttaga
    13321 ctggccc
//
LOCUS       DAECOD010000105        13247 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.105, whole genome shotgun sequence.
ACCESSION   DAECOD010000105 DAECOD010000000
VERSION     DAECOD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.105"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(13..996)
                     /gene="kdgT"
                     /locus_tag="KKO15_003176"
     CDS             complement(13..996)
                     /gene="kdgT"
                     /locus_tag="KKO15_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBD6361871.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1256..1876)
                     /gene="sodA"
                     /locus_tag="KKO15_003177"
     CDS             complement(1256..1876)
                     /gene="sodA"
                     /locus_tag="KKO15_003177"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBD6361872.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2161..3195
                     /gene="rhaT"
                     /locus_tag="KKO15_003178"
                     /pseudo
     CDS             2161..3195
                     /gene="rhaT"
                     /locus_tag="KKO15_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3192..4058)
                     /gene="rhaR"
                     /locus_tag="KKO15_003179"
     CDS             complement(3192..4058)
                     /gene="rhaR"
                     /locus_tag="KKO15_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBD6361873.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4114..4950)
                     /gene="rhaS"
                     /locus_tag="KKO15_003180"
     CDS             complement(4114..4950)
                     /gene="rhaS"
                     /locus_tag="KKO15_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBD6361874.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5238..6707
                     /gene="rhaB"
                     /locus_tag="KKO15_003181"
     CDS             5238..6707
                     /gene="rhaB"
                     /locus_tag="KKO15_003181"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBD6361875.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLATFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6704..7963
                     /gene="rhaA"
                     /locus_tag="KKO15_003182"
     CDS             6704..7963
                     /gene="rhaA"
                     /locus_tag="KKO15_003182"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBD6361876.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8205..9029
                     /gene="rhaD"
                     /locus_tag="KKO15_003183"
                     /pseudo
     CDS             8205..9029
                     /gene="rhaD"
                     /locus_tag="KKO15_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9039..9353
                     /gene="rhaM"
                     /locus_tag="KKO15_003184"
     CDS             9039..9353
                     /gene="rhaM"
                     /locus_tag="KKO15_003184"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBD6361877.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9654..10100
                     /locus_tag="KKO15_003185"
     CDS             9654..10100
                     /locus_tag="KKO15_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBD6361878.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10111..11562
                     /locus_tag="KKO15_003186"
     CDS             10111..11562
                     /locus_tag="KKO15_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBD6361879.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDISKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11552..12622
                     /locus_tag="KKO15_003187"
     CDS             11552..12622
                     /locus_tag="KKO15_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD6361880.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12622..>13247
                     /locus_tag="KKO15_003188"
     CDS             12622..>13247
                     /locus_tag="KKO15_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBD6361881.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3490 g         3141 t
ORIGIN      
        1 aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac
       61 tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt
      121 cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat
      181 cagcacaggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc
      241 atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac
      301 tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt
      361 attgcccagc gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc
      421 tcgtaattca gggtcaagat tccccagggc aaagcccacc aggaacggta atactgcgcc
      481 gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg cccagaataa tcatcgtcat
      541 gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt tcgtaccgta
      601 ctgctgcatg atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc
      661 cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga
      721 ggcaattgcc gcaaccaccc acgcgacggc aattttagtt accaccagcg taccggattt
      781 acgcagtacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac
      841 cgccagaatg ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc
      901 cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc
      961 ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt
     1021 taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca
     1081 ttcatttaat caataaaagt gatcaaaatc acatcaattg aaacgctgtt ttatttttat
     1141 aagaccattg tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt
     1201 cattttttaa gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt
     1261 ttttcgccgc aaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttga
     1321 tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc
     1381 ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt
     1441 tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac
     1501 caaagcggga agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt
     1561 cacgttcgat agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc
     1621 agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg
     1681 ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca
     1741 ggctttccag cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga
     1801 tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg
     1861 gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt
     1921 aagcagttgg ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact
     1981 tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta
     2041 atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca
     2101 cgatttttac actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag
     2161 atgagtaacg cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc
     2221 tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc
     2281 ggtgggattg tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat
     2341 ttctgggcgt attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc
     2401 gctatgtggg ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcatgtcg
     2461 atgggaattg gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt
     2521 atcaacggca atttcgatgt gctgattaac accgaaggcg gacgcatgac gttactcggc
     2581 gttctggtgg cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc
     2641 aagatgggca ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggtgatg
     2701 tgcggcattt tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa
     2761 gccgctgccg cacttggcgt cgatccactg tatgttgctc tgccaagcta tgttgtcatc
     2821 atgggcggcg gcgcgatcat taacctcggt ttctgtttta ttcgtctggc aaaagtgaag
     2881 gatttgtcgc taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta
     2941 ctctcggcac tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctagggccac
     3001 gcccgcattc cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta
     3061 ttgtgcggcg gtatcgtcgg gctggtgctg aaagagtgga acaatacagg acgccgtccg
     3121 gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc
     3181 ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg
     3241 gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa
     3301 atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc
     3361 tgccgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg
     3421 cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta
     3481 gccgccagcc gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa
     3541 tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc
     3601 atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc
     3661 cgcgcctgcg ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta
     3721 aatcccggaa tcgctccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata
     3781 atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaata
     3841 taaaagagat cgccacgggt aatgcgataa ggacgatcgt tgagtacatg cagaccatta
     3901 ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa gacatcttgc
     3961 ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga
     4021 agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca
     4081 atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg
     4141 gcgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact
     4201 gtcgctgaat ccacagcgat aggcgatgtc agtgacgctg gcctcgctgt ggcgtagtag
     4261 atgtcgggct ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg
     4321 ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc
     4381 ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga
     4441 tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat
     4501 aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat
     4561 ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg
     4621 atactgccca tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg
     4681 atccggcgag cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg
     4741 ccgatcatga tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt
     4801 aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg
     4861 ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt tacaagacgg
     4921 aaaaaaatcc acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga
     4981 aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaact acgtaaaaaa
     5041 cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca
     5101 caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt
     5161 ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa
     5221 ttcagcagga tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca
     5281 gtgggcgcgt gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa
     5341 tccatcgttt taacaatggg ctgcatagcc aaaacggtta tgtcacctgg gatgtggata
     5401 gcctggaaag tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg
     5461 atagcattgg gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc
     5521 gtgtgggcct gcccgttgct tatcgcgata gccgcaccaa tggcctgatg ccgcaggcac
     5581 aacagcaact cggcaaacgc gatatttatc agcgtagcgg catccagttt ctacccttca
     5641 atacgcttta tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg
     5701 ctcacgctct gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg
     5761 aatataccaa tgccaccacc acacaactgg tcaatatcaa tagcgacgac tgggacgagt
     5821 cgctactggc gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta
     5881 atgtcattgg gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca
     5941 gccatgatac cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc
     6001 tctcttctgg cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca
     6061 cggcgctggc agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga
     6121 aaaatattat gggcttatgg ctgcttcagc gagtgcttca ggaacggcaa atcaacgatc
     6181 tcccggcgct tatcgccgcg acacaggcac ttccggcctg ccgcttcatc atcaatccca
     6241 atgacgatcg ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg
     6301 aaacggcgca accgatcccg ggaagtgatg ctgaactggc gcgctgtatt ttcgacagtc
     6361 tggcgctgct gtatgccgat gtgttgcatg agctggcgca gctgcgcggt gaagatttct
     6421 cgcaactgca tattgtcggc ggcggctgcc agaacacgct gctcaaccag ctatgcgccg
     6481 atgcctgcgg tattcgggtg atcgccgggc ctgttgaagc ctcaacgctc ggcaatatcg
     6541 gcatcgagtt aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca
     6601 gcaccaccgc gaatctggcc acctttaccc ctaatcctga cagtgaaatt gcccactatg
     6661 tggcgcagat tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga
     6721 acaggcctgg gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc
     6781 gctgcgccaa cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc
     6841 cggttttgaa aacccggaag gttcgctgac cggggggatt caggccacag gcaattatcc
     6901 gggcaaagcg cgtaatgcca gtgagctacg tgccgatctg gaacaggcta tgcggctgat
     6961 tccggggccg aaacggctta atttacatgc catctatctg gaatcagata cgccagtctc
     7021 gcgcgaccag atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca
     7081 gctcggtctg gattttaacc cctcctgctt ttcgcatccg ctaagcgccg atggctttac
     7141 gctctcgcat cccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg
     7201 tcgcgtttcg gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat
     7261 cccggatggt atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc
     7321 agcactggat gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga
     7381 gagcaaattg tttggcattg gcgcagagag ctacacggtt ggctccaatg agttttacat
     7441 ggggtatgcc accagccgcc agactgcgct gtgcctggac gccgggcact tccacccgac
     7501 tgaagtgatt tccgacaaga tttccgccgc catgctgtat gtaccgcagt tgctgctgca
     7561 cgtcagccgt ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca
     7621 ggcaattgcc agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga
     7681 cttcttcgat gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa
     7741 aaaagccctg ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc tggaagcggc
     7801 gggcgattac actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgcggcaggc
     7861 ggtctgggaa atgtattgcc aacgtcacga tacgccagca ggtagcgaat ggctggagag
     7921 cgtgcgggct tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc
     7981 acgagcgcct tatccggcct acgggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg
     8041 ccctctccct gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca
     8101 ccgctccgga cagtcccctc gcccctacgg ggagagggtt agggtgaggg gaacaagccg
     8161 caccatgtct aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc
     8221 agtcctggtt tgtccaggga atgatcaaag ccaccaccga tgcctagctg aaaggctggg
     8281 acgagcgcaa cggcggcaac ctgacgctgc gcctggatga cgccgatatc gcgctttatc
     8341 acgacaactt ccacccacaa ccgcgctata ttccgctcag ccaacccatg cctttactgg
     8401 caaatacgcc gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagctcgatc
     8461 ctgcagctaa cttaggcgtc gtaaaagtcg acagcgatgg cgcgggctac catattctct
     8521 gggggttaac caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact
     8581 gcgagcgcat taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca
     8641 acctgatcgc cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt
     8701 gggaaggcag caccgaatgt ctggtagtat tcccggatgg cgttggcatt ctgccgtgga
     8761 tggtgcccgg cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc
     8821 tggtgttgtg gcccttccac ggtgtgttcg gttccggacc gacgctggat gaagcctttg
     8881 gtttaatcga caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg
     8941 gcatgaaaca gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta
     9001 caccactagc cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca
     9061 tgcaggtaaa ccccgacgcc cacgaagagt atcagcgtcg gcataatccc atctggccgg
     9121 aactggaagc agtgttgaaa tctcacggtg tgcataacta cgccatttac ctcgacaaag
     9181 cgcgtaatct gctgtttgcc atggtagaga ttgaatctga agaacggtgg aatgcggtag
     9241 ccagtactga tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc
     9301 cagataacag cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca
     9361 atgtgccgga gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat
     9421 tacatctccc ggtaagttat atttccctga tacattgtga gtaaatcaca aaaataatga
     9481 ataacccatt aatgattcat gtggtttatt taaataacca ttatgtgcat tactccgcaa
     9541 atctgacctt tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac
     9601 ttaatcgctc caccattaca attgattaaa atatatttta tggagtccga ttaatggcag
     9661 ctcttactgc aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccgttctga
     9721 agcagttggc gacaatagcg ttacaaaacg gttttatcac cgactcacac cagttcctgc
     9781 aaaccctgct gctgcgcgaa aaaatgcact ctactggatt tggttccggt gtcgcagtgc
     9841 cgcacggtaa aagcgcctgc gttaaacaac cgttcgtatt attcgcccgc aaagcgcagg
     9901 ctattgactg gaaagccagc gatggcgaag acgtcaattg ctggatctgc ctcggcgtgc
     9961 cgcaaagcgg cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc aaaattattc
    10021 accaggaatt tattcatcaa ctgcaacagg gcgataccga ccaggtgctg gccttgttaa
    10081 atcaaaccct cagctcataa ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc
    10141 accaactgcc ccgccgggat cgctcacacc tacatggtgg cggaagccct ggaacagaaa
    10201 gcccgttctc tcggccatac cataaaagtg gaaactcaag ggtccagtgg cgttgaaaac
    10261 cgcttatcca gcgaagagat tgccgctgcc gattacgtca ttcttgctac cgggcgtggc
    10321 ctgagcggtg atgatcgcgc gcggtttgcc gggaagaaag tttatgagat tgccatctcc
    10381 ctggcgttga aaaatatcga ccagattttc agcgaattac cgacaaactc gcagcttttt
    10441 gccgcagata gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc
    10501 cacctgatgg cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg
    10561 gcgcttgcca acatgctggt gcagttcggt ttgccgtata ccgatatttc gaaaggcgcg
    10621 ccgtcgttta cctgggtagt tgaatccatc ggttaccttg gcttcacctt tatgatcccc
    10681 atcatgggcg cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt
    10741 ctggtttgct atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc
    10801 ctcggcgcgg tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgcaaagtg
    10861 cgccttggca aagcattaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg
    10921 ctggttttcg gcgtgctcac ttactacgtt atcgggccgg tgatgtccga cctcatgggc
    10981 gggctgctcc acttcctgaa taccattcct ccatcaatga agtttgcggc agcgtttctg
    11041 gtcggtgcga tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc
    11101 tgcttctcac tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg
    11161 ttgatgccgc ccgttgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga
    11221 caggaaaaag aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg
    11281 gctatacctt acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggc
    11341 ggcattaccg gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc tggtttgggt
    11401 atctttgacc cgctgatcgg cctgatgtcg ccggtaggtt cgttttatct ggtgctggcg
    11461 attggcctcg cgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc
    11521 aaagcgaaag ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg
    11581 cgaagccagc gccgttagcg gcgatgaaca ggaagttcgc gacatcctga taaacacgct
    11641 ggaaccttgc gttaatgaaa tcacctttga tggtctgggc agctttgttg cccgtaaggg
    11701 aaataaaggt ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac
    11761 ccacatcgac gagagcggtt ttctgcgttt caccaccatt ggtggctggt ggaatcagtc
    11821 gatgctcaac catcgggtaa ccatacgcac acacaaggga gtgaaaatcc ctggtgtgat
    11881 tggtttcgtc gcgcctcatg cgttaacgga aaagcaaagg caacagccgc tgtcatttga
    11941 tgagatgttc attgatattg gcgcaaacag tcgcgatgag gtggaaaaac gcggtgtggc
    12001 gatgggcgat tttattagcc cggaagccaa ttttgcctgc tggggcgaag ataaagtggt
    12061 cggcaaggcg ctggataacc gcatcggctg cgcaatgatg gctgaactat tgcagacggt
    12121 gaataatccc gaaattacgc tgtatggcgt cggcagcgtg gaagaagaag ttgggctacg
    12181 cggggcacaa acctcggcgg aacacattaa accggatgta gtgatcgtgc tggataccgc
    12241 tgtcgcaggc gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg
    12301 gccggggctg atgctgtttg acaagcgcta cttccccaac cagaaactgg tagcagcgtt
    12361 aaaaaactgt gccgcacata acgatttacc gctgcaattt tccaccatga aaaccggagc
    12421 gacggatggc gggcgctaca acgtgatggg cggcgggcgt ccggttgttg cgctgtgcct
    12481 gccaacccgc tatctgcacg ctaacagcgg catgatttca aaagccgatt acgatgctct
    12541 gctcacgttg atacggggtt ttctgacgac cttaaccgcg gagaaagtca acgcgtttag
    12601 ccagttccgt caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg
    12661 gagcaacagc cgcgcacgcc tggtgagctg gcgcaacaga ctggcgtttc aggcaggacc
    12721 atcctgcgtg atattgacta tctcaacttt acccttaacg gcaaagcccg cattttcgcc
    12781 agtggcagtg cgggctatca gctggaaatc ttcgagcgcc gcagcttttt tcagttgctg
    12841 caaaagcacg ataacgacga tcggctgctg gcgctgttat tactgaatac tttcactccc
    12901 cgtgcgcaac tcgcctcggc gcttaatttg ccagaaacgt gggtagcaga gcgtctgccc
    12961 cagttaaaac agcgttatga acgcacttgt tgcctggcca gccgccctgg tttgggccat
    13021 ttcattgatg agacagaaga gaaactcgtt atcttgctgg cgaacttgct gcgcaaagat
    13081 ccgtttttaa ttccgctggc gggcataaca cgagacaacc ttcagcattt atccacggcc
    13141 tgcgacaacc aacaccgctg gccgctcata cagggtgatt atctctccag cctgattctg
    13201 gcgatttacg ccctgcgtaa tcaactgacc gatgagtggc cgcaata
//
LOCUS       DAECOD010000106        12733 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.106, whole genome shotgun sequence.
ACCESSION   DAECOD010000106 DAECOD010000000
VERSION     DAECOD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.106"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1038
                     /locus_tag="KKO15_003189"
     CDS             <1..1038
                     /locus_tag="KKO15_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD6361882.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="KKO15_003190"
                     /pseudo
     CDS             1039..3204
                     /locus_tag="KKO15_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            3218..4129
                     /locus_tag="KKO15_003191"
     CDS             3218..4129
                     /locus_tag="KKO15_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HBD6361883.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="KKO15_003192"
     CDS             4129..5424
                     /locus_tag="KKO15_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBD6361884.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="KKO15_003193"
     CDS             5469..5699
                     /locus_tag="KKO15_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361885.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="KKO15_003194"
     CDS             5677..6177
                     /locus_tag="KKO15_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HBD6361886.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLIATARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="KKO15_003195"
     CDS             6177..7595
                     /locus_tag="KKO15_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBD6361887.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIVSASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="KKO15_003196"
     CDS             7595..8443
                     /locus_tag="KKO15_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361888.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="KKO15_003197"
     CDS             8443..8898
                     /locus_tag="KKO15_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBD6361889.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSGTREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="KKO15_003198"
     CDS             8901..9593
                     /locus_tag="KKO15_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBD6361890.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="KKO15_003199"
                     /pseudo
     CDS             9603..10934
                     /locus_tag="KKO15_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            10935..12665
                     /locus_tag="KKO15_003200"
     CDS             10935..12665
                     /locus_tag="KKO15_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD6361891.1"
                     /translation="MVNYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPRTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQAPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3434 a         3141 c         3356 g         2802 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc gggggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatgag acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tcctgaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tatagccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg tcagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaagcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcggcacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taaggggtaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgactag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggtt aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctaggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga cgctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agcccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAECOD010000107        12695 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.107, whole genome shotgun sequence.
ACCESSION   DAECOD010000107 DAECOD010000000
VERSION     DAECOD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.107"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="KKO15_003201"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="KKO15_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBD6361892.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="KKO15_003202"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="KKO15_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBD6361893.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="KKO15_003203"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="KKO15_003203"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBD6361894.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="KKO15_003204"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="KKO15_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD6361895.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="KKO15_003205"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="KKO15_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBD6361896.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="KKO15_003206"
     CDS             4025..4615
                     /locus_tag="KKO15_003206"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBD6361897.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="KKO15_003207"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="KKO15_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBD6361898.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="KKO15_003208"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="KKO15_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBD6361899.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="KKO15_003209"
     CDS             complement(6551..6658)
                     /locus_tag="KKO15_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361900.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="KKO15_003210"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="KKO15_003210"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD6361901.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="KKO15_003211"
     CDS             7648..7875
                     /locus_tag="KKO15_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBD6361902.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KKO15_003212"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KKO15_003212"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBD6361903.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="KKO15_003213"
     CDS             complement(9447..10703)
                     /locus_tag="KKO15_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBD6361904.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="KKO15_003214"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="KKO15_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBD6361905.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="KKO15_003215"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="KKO15_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBD6361906.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KKO15_003216"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KKO15_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD6361907.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3244 c         3198 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgtcctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcattggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAECOD010000108        12219 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.108, whole genome shotgun sequence.
ACCESSION   DAECOD010000108 DAECOD010000000
VERSION     DAECOD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.108"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..492)
                     /locus_tag="KKO15_003217"
     CDS             complement(<1..492)
                     /locus_tag="KKO15_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6361908.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="KKO15_003218"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="KKO15_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBD6361909.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="KKO15_003219"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="KKO15_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD6361910.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="KKO15_003220"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="KKO15_003220"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBD6361911.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="KKO15_003221"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="KKO15_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBD6361912.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="KKO15_003222"
     CDS             3646..4155
                     /locus_tag="KKO15_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6361913.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="KKO15_003223"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="KKO15_003223"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBD6361914.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="KKO15_003224"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="KKO15_003224"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBD6361915.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="KKO15_003225"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="KKO15_003225"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBD6361916.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="KKO15_003226"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="KKO15_003226"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBD6361917.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="KKO15_003227"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="KKO15_003227"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBD6361918.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="KKO15_003228"
     CDS             complement(8797..9690)
                     /locus_tag="KKO15_003228"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD6361919.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="KKO15_003229"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="KKO15_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="KKO15_003230"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="KKO15_003230"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBD6361920.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="KKO15_003231"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="KKO15_003231"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBD6361921.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2967 a         3156 c         3117 g         2979 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggcctgg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atcgggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga taaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaagtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg cagaaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAECOD010000109        12153 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.109, whole genome shotgun sequence.
ACCESSION   DAECOD010000109 DAECOD010000000
VERSION     DAECOD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.109"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            126..995
                     /locus_tag="KKO15_003232"
     CDS             126..995
                     /locus_tag="KKO15_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBD6361922.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="KKO15_003233"
     CDS             complement(1198..1551)
                     /locus_tag="KKO15_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBD6361923.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KKO15_003234"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KKO15_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBD6361924.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="KKO15_003235"
     CDS             complement(3379..3672)
                     /locus_tag="KKO15_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBD6361925.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="KKO15_003236"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="KKO15_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBD6361926.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KKO15_003237"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KKO15_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBD6361927.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="KKO15_003238"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="KKO15_003238"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBD6361928.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="KKO15_003239"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="KKO15_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBD6361929.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="KKO15_003240"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="KKO15_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBD6361930.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="KKO15_003241"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="KKO15_003241"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBD6361931.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="KKO15_003242"
     CDS             11052..11627
                     /locus_tag="KKO15_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6361932.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="KKO15_003243"
     tRNA            11734..11809
                     /locus_tag="KKO15_003243"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2981 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tagagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tcttccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DAECOD010000110        12136 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.110, whole genome shotgun sequence.
ACCESSION   DAECOD010000110 DAECOD010000000
VERSION     DAECOD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.110"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..397)
                     /locus_tag="KKO15_003244"
                     /pseudo
     CDS             complement(1..397)
                     /locus_tag="KKO15_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            521..1261
                     /locus_tag="KKO15_003245"
     CDS             521..1261
                     /locus_tag="KKO15_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HBD6361933.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(1382..3361)
                     /locus_tag="KKO15_003246"
     CDS             complement(1382..3361)
                     /locus_tag="KKO15_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBD6361934.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAEYHELYCAGHLIEAGVAFFQATGRRRLLE
                     VVCRLADHIDSVFGPNEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SYASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(3372..4772)
                     /locus_tag="KKO15_003247"
     CDS             complement(3372..4772)
                     /locus_tag="KKO15_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361935.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGHGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4998..5813
                     /locus_tag="KKO15_003248"
     CDS             4998..5813
                     /locus_tag="KKO15_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6361936.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5845..6540)
                     /gene="araD"
                     /locus_tag="KKO15_003249"
     CDS             complement(5845..6540)
                     /gene="araD"
                     /locus_tag="KKO15_003249"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD6361937.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETYEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6534..7394)
                     /locus_tag="KKO15_003250"
     CDS             complement(6534..7394)
                     /locus_tag="KKO15_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBD6361938.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAAAQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKALHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(7387..8049)
                     /gene="ulaD"
                     /locus_tag="KKO15_003251"
     CDS             complement(7387..8049)
                     /gene="ulaD"
                     /locus_tag="KKO15_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD6361939.1"
                     /translation="MSRPLLQLAPDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(8046..9542)
                     /gene="lyxK"
                     /locus_tag="KKO15_003252"
     CDS             complement(8046..9542)
                     /gene="lyxK"
                     /locus_tag="KKO15_003252"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBD6361940.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVFTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTLAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9546..10532)
                     /gene="yiaO"
                     /locus_tag="KKO15_003253"
     CDS             complement(9546..10532)
                     /gene="yiaO"
                     /locus_tag="KKO15_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBD6361941.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEAITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(10545..11822)
                     /gene="yiaN"
                     /locus_tag="KKO15_003254"
     CDS             complement(10545..11822)
                     /gene="yiaN"
                     /locus_tag="KKO15_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBD6361942.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11825..>12136)
                     /locus_tag="KKO15_003255"
     CDS             complement(11825..>12136)
                     /locus_tag="KKO15_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBD6361943.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT         2892 a         3204 c         3250 g         2790 t
ORIGIN      
        1 agattgcagg tttttagcga taaggtgata tcaccgatat ctttaatttt atcgattaca
       61 tgtttggata aataggtaat tatgccggtt ggggcgtaga tggtgacccg accacgtttc
      121 tcatgcttat aatctgcaat aaaattatta agttgctcgt ttctgtccag catgtcgttg
      181 atgtacggta acagcgcggt gccaaaaggt gtgagcatca gctgccgggt ggttcggtca
      241 aagactttta aacccacttt tgattcaaaa tcagcaagat atttgctgac gttggcctgt
      301 gcgatgccag gtacagtccg gcatggctga tattttcatt ggcagcgatt accgagataa
      361 tttttaactc ccggtactta agttgtaatt ttgtcatcgc aacagcccaa ttatatataa
      421 ttttatatat tactatataa atatgaatct tgcaccggag aaattgtaag cattattatg
      481 ccgactcttt tttatcttat tttaaaatgg aataccggac atgttaatta atcgcaatat
      541 tgtggcgtta tttgcgttgc cttttatggc aagcgcaact gcttctgaat tatccattgg
      601 tgctggtgca gcttataatg aatcgcctta tcgcggttat aatgaaaata caaaggcaat
      661 tccgctgatt agttatgaag gtgattcttt ttatgttcgt cagaccacat taggttttat
      721 tctgtcgcaa agtgaaaaaa atgaacttag cctgaccgca tcctggatgc cgctggaatt
      781 tgaccctgcc gataatgacg attatgccat gcaacagctt gataagcgtg atagtacggc
      841 tatggcgggg gttgcctggt atcaccacga gcgttgggga accgtgaaag cctctgcggc
      901 tgcggacgtt ctggataaca gcaacggctg ggtgggggag ctatcggtat tccacaaaat
      961 gcagataggt cgcctgtcgc tgacacctgc gctgggcgtt ctctattatg acgagaattt
     1021 cagtgactat tactatggca tttcagagag tgagtcccgt cgtagcggtc tggcaagtta
     1081 ttccgcgcag gatgcatggg tgccctatgt cagcctgacg gcaaaatacc cgataggaga
     1141 gcacgtcgta ttgatggcga gcgccggata cagcgagctg ccggaagaga ttaccgacag
     1201 cccgatgatt gatcgtaatg agagttttac ctttgtcacc ggcgtgagct ggcgttttta
     1261 attcaccggt ggatgtcggt gcggctggcc tacgctctcg caccgactcg tatgcctgat
     1321 aagacgtgtt aagcgtcgca tcaggcaatg tgctccctac gttggatgcg gcgcgagcgc
     1381 cttatccaac ctccggatgg cgatgctttt cctcattcac ccagatccgc atttcgcctt
     1441 cgccccggtt agcccagcta aaccacggga taaaggtcag agtttgcggc tggcgtttgg
     1501 ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg attgctctgt tcataccggt
     1561 aacccggtgc ctggattaag atcttatggc tgaacaatcc cttgccttca aatgtcgtaa
     1621 atggcgcatc ggtgggcagc cacagattat gcagtgactc gccgttgtcg gcctgttcta
     1681 ggcaatacac cagcggaccg cgctgaatcg ccacttttcc ggcgacgtga cgcaccagcg
     1741 ggttaccgta aacgcggcgt accggcatcg gcaaagtcag attcagcgta tcgccctcct
     1801 gccattcgcg ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc tcttccccat
     1861 tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg taaagccagc gtatgacgta
     1921 ccggctgggg cgattcaacc gcaatcgtca cctgctcctg ccacggatag ttcccgctaa
     1981 cccgcaggcg cagcgtgcca ttttctaccg gcacttccat gctgtttcct gcgtatatgt
     2041 tgatatacaa cgcatcttca cgcggcgtgt agagataatg accaatcgag gtcagcacgc
     2101 gggcgatatt tggcggacaa caagcgcagc caaaccaacg ctggcggatc ggtttaacgt
     2161 gatcgtagat atggttgaat ttcagcgatt ttggatgtac ttccagcgga ttcacataga
     2221 agaaatgttt gccatccagc gccatgccgc cgagcacggt gttgtacagc gcgcgctcca
     2281 tcacatcggc atattgactg tcgccttcca tttccagcat tcgccgggcg aacatcatca
     2341 ggccgatgga agcataactt tcggcgtaaa ccgtgtcatt cggcagatcg taatcgctag
     2401 tgaacgcttc gccgctgctt tgcgagccaa tgccgccggt aatatataac tgacgctggg
     2461 ccatattgtt ccacagcctc aggcagtcct gacgcttgct gtcatcgtga cttaaacgcg
     2521 cgagatgcgc gacgccggtc atcaggtaga caaaacgtac cgcgtgaccg atggcggttt
     2581 gctgttgcgc aaggggcaaa tgtgcctggc tgtaggcttt gtctttcacc atccacgccg
     2641 ggccgtaggt gtgccagtgc gaggtctgcc cgcgcttttc atattcctgg tcgtaatagt
     2701 gcggttgcgc accacgctgt tcgacaaaat agttcgtcag cgccaggtag cgcggctctt
     2761 cggtgacttc gtagagacgc atcagcgcca gttcaatttc cggatgacca ggataaccct
     2821 gcagcttgtc ttcatttgga ccaaatacgc tgtcgatatg atcggccaga cggcaaacca
     2881 cttccagcaa gcgccgccta cccgtggcct ggaagaaggc gactccggct tcaatcagat
     2941 gaccggcgca gtaaagttca tgatactccg ccagattgct ccagcgttct tcgggtgctt
     3001 ttaccgtaaa gtaagtattg agatagccgt cttcacattg ggcggaggcg atcagttcga
     3061 ttacctcgtc ggcggttttt tccagttcgg cgtccggctt ctggcacagc gaccaggcta
     3121 ccgcttccag ccatttggcg acgtcgctgt cctgaaacac catcccgtaa aattcaccct
     3181 cctgaagtcc ggcagcaatg cgaaagtttt caatcgcatg gctgggttcc gcttctggga
     3241 tacgatcgtt caaggcatcc cactgataag gaatcaccac gtcgcggacc agttgttggt
     3301 actgaccgag gaacggatcg ctgaccgtca gtttacgcag atcgacttcc gaaatgttca
     3361 tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt cggtagctat gcgtgacatc
     3421 ataggattat tgagtttgca aaagcggatc gcccctgcca gcagcaggtg gaatagcgca
     3481 ggcagtaatg tttccatcgc cgcgatcccg ccaagcgaac tggcggtctg gttttccacg
     3541 ccaggctggt aggcgacaaa aataaacagc aggctgatga tcccggcgct ggaggcccag
     3601 gccagcttga tgaaaaacag attgaaggcg aagttcatgc cggaagaacg tacgcgggtt
     3661 ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga agtgcaatgg caatgtgaag
     3721 ccaagaatca cgccattacc gaggatcacc gccagccaca gagtttgata agccgggccg
     3781 gagggcagga accacatcaa caccgccagt gcagcgagga gcaggttggt gtagtaataa
     3841 attttgacgg tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc gataatggag
     3901 gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg tactgccttg taagacgtag
     3961 gtaatgaagt acatataccc accgccgcga atgttaaaga cgttaatcag caggaaagac
     4021 atgaccagca tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg ttcgcgcagg
     4081 gtaaatttcc ccattgtgga gagcggcacc cgttcacgaa cccagaagaa acagcacaga
     4141 aacatcacca cggcgatggc gcacagcacg ccgacgccca attgataacc gcgcgcggca
     4201 ttaccatgac cgaagagatc taccatccac ggtaagccta ccgaaaccag aaatcccgct
     4261 acgccacaga gaacgaatcg ccaggactgg caggagatca cttcattgtg gcgggtggtc
     4321 atggtgttga tcagcgcgca gtacggtacg ttgatggcgg tataaccgac tgaaaggagc
     4381 aggtaagtac cgaacgccca ggcgattttt actcccatgc tggcatctgg cacggtgaac
     4441 gtcaggatgc cgataatgcc gatgggaatg gcaatccata gctgccacgg acgaaagcgc
     4501 ccccaacgac tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga gatggcatca
     4561 aacacgcgca gtgcaataaa cagcgtgcca accagcgccg gagttaatcc aaaaacgtcg
     4621 gtgtagaaaa aggtgagaaa attcatgatc aggcaagtta ttacggtgcc gcctgcgtca
     4681 cccaggccgt agcctatttt ttcccgtaca gataacgcat cgtcaatttt accgttagcg
     4741 atatcagcgg tagtaatcgg tgtagaggtc atagatattc ctcgtgaata gggagcaatt
     4801 tttagtttta ttgaaatgac ctgattaatt ctgccgtgaa cgggaatatc aacaaggcga
     4861 gaaaagagta aaaaaaggtg acgattccga cctgaattta aaagtgtgaa atggatcagg
     4921 tgaaattaat gtttttgatt aataatcaaa aggtaatgta tttaatcatt aatgaataag
     4981 cgggaaatta catatcaatg ctcgaattat ccatgacgtt accgattaaa gtgcaaaatg
     5041 gtggattatt tatttcccgc ggcgtaggga ggcatcctgc aaggcggctg gagtcatggg
     5101 aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga agaagaacgg atatttctgg
     5161 tcgatgcggg agagagttta ttactctggc cgaatcgaca acatgttggg gtggaggatt
     5221 ttcccgccga tctcaaattt tactggctgc attttgaggt taaagcgccg gatagcgacc
     5281 cgcgctggct cactcatctt agcgtgccgc aacaaacgaa ggttgccgat ccgcaggcgc
     5341 tggtggccct gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc actccggcgc
     5401 tggagtttat tgtgctgctg attttgcagc agctcacccg tgacgcgcgt cttgatgaac
     5461 aggccgatgg cgcaggcacc acccttgcct ggaaagcgca gaaactgatc cgcacgcaat
     5521 atcatcgccc gctttctacg gcgcttctgg cccgcgagct gtactgcaat gccgattatc
     5581 ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac agaagcaatc catcgccagc
     5641 gagtgcgcga agccgaaaag ctgttaatta gcgaagcgcg atcgctcact gaagtggctg
     5701 cgttgtgcgg gtttaatgac gttggctatt tccggcagat ttttcagaaa catactggtt
     5761 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg
     5821 gcgatgaagc ccctgcgtga gggattactg cccgtaatag gcattggccc catgcttacg
     5881 caggtagtgc ttatccagca gttcgttttg catcgcaggg agctgcggcg caagctggcg
     5941 cgagaatagg cccatatagg cgcattcttc gagtactacg gcgttatgca cggcatcggc
     6001 ggcgttttta ccccatgcga acgggccgtg agaatgcacc agcaccgccg ggatttgtgc
     6061 cggactcctg ccacgttctt cgtaggtttc aatgatcact tcgccggtct gatattcata
     6121 ttcgccgtta atctcctctg cggtcatctg tcgcgtgcag gggatggcac catagaaata
     6181 atcagcgtgc gtggtgcccc aggcggggag atccagcccg gcctgcgacc agatggtggc
     6241 gtggcgcgag tgggtatgca caatgccacc gatttcggcg tagcgacggt aaagcgccag
     6301 atgcgttggc gtgtcggacg agggcttttt gctaccttcc accaccttgc cgctggctat
     6361 ctcaaccacc accatatcgt cggcggtcat cacgtcatac tcgacgccgg aaggtttgat
     6421 taccatccat tgccgcgttt cgtctaccgc gctgacattg ccccaggtga acgtcaccag
     6481 atggtgagcg ggaagcgcca gattcgccgc cagcacgtcg gctttcagtt gctctaacat
     6541 ataaatccag cctcctgcat acgcgcttca atccaacgcc gcgcctgaat aatctccagc
     6601 accggctctt tggctttttc ggtccacatc tcaatcagga aagaacctcg atagttcagt
     6661 tcatgcagcg ctttgaagat gccaacgaaa tcgacgcagc cttcgccaaa cggcacgtcg
     6721 cggaactgtc cgggactttg ctcggtaact ggctgggtgt ctttcaggtg gatcgcggcg
     6781 atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc gctgaggttg
     6841 ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca ctttttccat
     6901 ttactgatgg agttcataaa cgcggtgtcc ataatctcca ccgccagcat cacctgcgcg
     6961 gctgctgcct gttcaaccgt ccacgccagc ccttcagcaa aacgttgccg ggtgccttca
     7021 tcgtggtctt cgtaatagac gtcgtagccc gccagttgga tggtgcgtat gccgagatcg
     7081 cgtgccagac gaatcgcttt gctcatgatc tcccgtgctc gctggcgtac cgcgtcgtca
     7141 cggctaccaa aaggaaagcg acgatgtgcg gacaggcaca tcgacgggat cccaacaccc
     7201 gtttcgatca tcgcggcaac cagcgaagtc ctttgtgcgg tgctccagtc aagacgtgaa
     7261 agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaaaccgca gctttttgcc
     7321 agcaccagcc gctccggcca ggagagatct ttcgccagcg ccttttcata aatccctaac
     7381 ggatgattac gcacgcttgc ctccccagat agcgtcgatt tgcgcatgga aatcgccagc
     7441 cacctgcgcc ggatttgctg agcctgccag tgctcgcccg gcaataaacg ctttcacgcg
     7501 aatatcttta aacagcggca ggtcagcagg ggtaatcccg ccggtaatgg aaagctcaag
     7561 gccgatatcg gaaagcgcct tcatgcgtgc cagatcggct tcgccccact gttgcccgct
     7621 ggcctgtgca tcacgaccgc gatgataaat cgcctgccgc acgccaatac gatgccagtc
     7681 gcgggcgtcg tccagcgtcc agttaccgaa caactcgatc tgaatttcac caccgcagcg
     7741 ttgcgccatc gtgtggcctt tttccaccgt cgcgagcggc gcggcgcaga tgatggtcat
     7801 ccagttagcg cctgcgccaa acgcctgttg cgcgagcgtt tcaccagcgt cggcgacctt
     7861 ccagtcggca acgatgattt tgtccgggca ttgttcgcgc aaggctttca ccgcgctaag
     7921 cccttcgttt aaacagagaa tggtgcccgc ttcgacgata tcgacgctgt cttttaacag
     7981 cgtcacgtcg cgctgcgcgg cttcaagtga tgagtggtcg ggtgccagtt gcagaagtgg
     8041 tcggctcata atgtgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc ggcaatgaga
     8101 tgctgatagc gttggtattt ttgttggtaa agctgatgcg cggtcatatc cggcagcagg
     8161 gtgcgtaccg ggtgctgtaa gtcacgttga gcttcgctga aatcgcgata aactccggtg
     8221 ccgacgcggg cggcaagtgc cgcgccaaaa cagccggttt cttccacctg cggcagctcg
     8281 atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggagtg cgccgggccg
     8341 ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt gaggtgggtc
     8401 atatggctaa acaccacccc ttcatagatg gcctgcaaca ggtgcgcgag ggtgtgaatg
     8461 gcctgcatcc cgtagaaacc gctggtcatc tcgagtccgg cgttgctgcc gtacaggaac
     8521 ggcaggaaaa agagatcgcc accgaccttc ggcaagctgg caacggcctg attgatctca
     8581 tcaaacgaga tttctcccca ctgcgcggta aaccattcga ggttgccgga agaggtaggg
     8641 ctggcttcgt gaacgataaa ttgaccatcg ttaacgtagc gaccatagac atacggatgc
     8701 gcttcaccgt cacgtaaacc gcgggttatg ccgctggtca ccgcccaggt gcccatcact
     8761 gcgttgaggg tgaattcgtc ttcgatcccg gcgcagagtg cggtgaaaac cacatcaaac
     8821 aggccgccga ctacgggcgt acccgctttc agaccggtca gtgcggctgt ctgagcggtg
     8881 atttccccgc agatttcggc agaaccgaca acaggcggca gggcgtgatt gatttcagcg
     8941 atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt gtagaggttg
     9001 gactcggaaa tattgctctc ttcacagcct ttgacgccgg ttaaacacca gcgcaggtag
     9061 tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ctggttcgtg ctctttaagc
     9121 caacgtagca gcgacaccgg atgcccggtc cacaaggttt gtcgggtcag cgggtagagt
     9181 ttttccggga tgccatcttc ttgccagcga cgaacgattt ccatcgcccg gcgatccgag
     9241 gacaaaatgg cgttaccgag cggcttgttg tttttatcca gcaaaaacaa gccttttcct
     9301 tgtgcggaga taccgatacc gacaatttgt tccccgctaa cgccggaatg ggtaagcagt
     9361 tcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc tgcccaacct
     9421 ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg gccttcgcgg
     9481 tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca gtattgcgtc
     9541 atggtttatt gcacctcatc cacggctttc agtagatcag caccctgcgg aacatctttt
     9601 acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg atcgagatcg
     9661 gtgatggctt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg atcttcggca
     9721 accagtttgc gctggtagtt acccgcttcc tgcgcagatg aaattagcgc ttgctggaac
     9781 tccgggctta agccatcaaa cttcgctttg ttgatcacca ccagaagcgg ggaataggcg
     9841 tggtgcgtca gagaaaggta cttctgcact tcgaaaaatt ttgctgacca gacgacgttg
     9901 atcgggtgtt cctgagcgtc gatagtgcgg gtttccagcc cggtatagac ttcggcaaac
     9961 ggcatcggga tcgggttagc gccaaagact ttgaatgcgg ctatattcat cgggctattg
    10021 ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc gcgcgagttg
    10081 gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt accttcaagt
    10141 gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg agcggtatcg
    10201 cggaacagga aagggacatc aagcaagttc atcactggtg ataacccggc aaagttattc
    10261 gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat cgcctgcgcg
    10321 ttaccgaggg tgctatccgg aaacaatttt aatttcagct cgcctttggt tctctcctgc
    10381 aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga ggtttcataa
    10441 ccgaaacgta aagattgcgc cgccagagaa gatgtgctga atgcagccag gccagcaatg
    10501 aataatgcgt aggttacaga gcgtaatttc ataatatttt ccttttaatt aatccatttc
    10561 aaagggagga tgatgagatc gggaataaaa acaaatacga ctaataatga atataaaacc
    10621 aggacgtaag ggaaaacgcc tctgactgca tcatcgaatt tgagttttgc cacccctgaa
    10681 ataacgttaa gtacattacc gataggcggt gtaattaagc cgattgagca gttaatgata
    10741 aacatgacac cgaaataaat cggatcgatt cctgcctctt taactaaagg cattaatacc
    10801 ggggtaagaa ttaataccgt cggcgttaaa tccatgacca tgccgacaat caggatagcc
    10861 accataatga cgataaacag cagacgcggt gaatcgacca acggttgcag taaatcagaa
    10921 accatcatcg gcagttcagc aatggtaatc agccaggcgg aaacttgtgc cgaggccacc
    10981 agaaacataa ccactgaggt ggttttcgcc gcgccaatga gtacatgcca aagtgtggcg
    11041 aaggtcattt cacggtaaat aactgtggcg acaaacagcg catagaaagc agcaacggcc
    11101 ccggcttcgg taggggtaaa cagaccggag cgaaacccgc caataatgat gactggaaga
    11161 aacagcgccc agataccgga gacaaaagag tgccagattt cctgcatcgt tgctttttgc
    11221 tggcggggta aattaaggcg actggcctgc cgccaccagg taagcatcag cgttgcgccc
    11281 atcattatgc caggggcaat gcccgccata aacagcttac tgatggataa tccgctggaa
    11341 acaccgaaga taataaacgg aatggaaggg ggaataattg gcgcgataat gccgccagag
    11401 gcaatcagcc ccgccgcccg gttgaccggg tagttggcgc tgcgcatcat cggcaccagt
    11461 agagcggcaa cggcggcggt atccgcaacg gcagaaccag aaaggctcgc cataatcata
    11521 gctgccagca cgccgacgta gcccagcccg cctggtttat gccccaccag tttcatcggc
    11581 aggtcaacaa tgcgttttaa caagccgccc gcattcatga tttcacccgc cagcacaaag
    11641 aacggaatcg ccaggaggga gaagctatcg gctccgttca ccagcgtttg tgccatgatc
    11701 tggacatcaa acatgtccag ccagaacatt aacgccgccc cgcacaacaa cagtgcccag
    11761 gcaataggca aaccgatagc aataccaccc aacagacagc ccagaaaaat cagcacagcc
    11821 atgattaagc tccttgcggt ggcgacgtta gagaattgct acgcgtgatg agttgatata
    11881 aatgacgcag ttcaaagaat gcgataacga ggctggtggg cagacaagcg gcatacatca
    11941 ggccgatggg caaaccgagg atcggtgaat aatcgctcca gtcctgaatt gtttttagcg
    12001 ttgcgcccca ggccagtgcg ccacaaataa ataagattaa ggaatgggta accagagcga
    12061 ctcgtcgttg ccatgcgggg gagagtttct ccaccagaaa ggtgacctga acgtgggcgt
    12121 tatccataaa agccac
//
LOCUS       DAECOD010000111        12112 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.111, whole genome shotgun sequence.
ACCESSION   DAECOD010000111 DAECOD010000000
VERSION     DAECOD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.111"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KKO15_003256"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KKO15_003256"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBD6361944.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KKO15_003257"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KKO15_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBD6361945.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KKO15_003258"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KKO15_003258"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBD6361946.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KKO15_003259"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KKO15_003259"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBD6361947.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KKO15_003260"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KKO15_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBD6361948.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="KKO15_003261"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="KKO15_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBD6361949.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVVWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="KKO15_003262"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="KKO15_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBD6361950.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="KKO15_003263"
                     /pseudo
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="KKO15_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="KKO15_003264"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="KKO15_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBD6361951.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="KKO15_003265"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="KKO15_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBD6361952.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="KKO15_003266"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="KKO15_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBD6361953.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12112)
                     /locus_tag="KKO15_003267"
     CDS             complement(11912..>12112)
                     /locus_tag="KKO15_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBD6361954.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2781 a         3389 c         2932 g         3010 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacaccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctag cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga ag
//
LOCUS       DAECOD010000112        11937 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.112, whole genome shotgun sequence.
ACCESSION   DAECOD010000112 DAECOD010000000
VERSION     DAECOD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.112"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..340
                     /locus_tag="KKO15_003268"
     CDS             <1..340
                     /locus_tag="KKO15_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6361955.1"
                     /translation="LQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSA
                     KGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLN
                     PEQFENQNLA"
     gene            complement(588..663)
                     /locus_tag="KKO15_003269"
     tRNA            complement(588..663)
                     /locus_tag="KKO15_003269"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Ile,seq:cat)
     gene            1222..3474
                     /locus_tag="KKO15_003270"
     CDS             1222..3474
                     /locus_tag="KKO15_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361956.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3810..4787
                     /gene="csiD"
                     /locus_tag="KKO15_003271"
                     /pseudo
     CDS             3810..4787
                     /gene="csiD"
                     /locus_tag="KKO15_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
     gene            4807..6075
                     /gene="lhgO"
                     /locus_tag="KKO15_003272"
     CDS             4807..6075
                     /gene="lhgO"
                     /locus_tag="KKO15_003272"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBD6361957.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            6098..7546
                     /gene="gabD"
                     /locus_tag="KKO15_003273"
                     /pseudo
     CDS             6098..7546
                     /gene="gabD"
                     /locus_tag="KKO15_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7554..8840
                     /gene="gabT"
                     /locus_tag="KKO15_003274"
     CDS             7554..8840
                     /gene="gabT"
                     /locus_tag="KKO15_003274"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBD6361958.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAQDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            9078..10478
                     /gene="gabP"
                     /locus_tag="KKO15_003275"
     CDS             9078..10478
                     /gene="gabP"
                     /locus_tag="KKO15_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBD6361959.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10499..11161
                     /gene="csiR"
                     /locus_tag="KKO15_003276"
     CDS             10499..11161
                     /gene="csiR"
                     /locus_tag="KKO15_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBD6361960.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(11162..11611)
                     /gene="kbp"
                     /locus_tag="KKO15_003277"
     CDS             complement(11162..11611)
                     /gene="kbp"
                     /locus_tag="KKO15_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBD6361961.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11695..11853)
                     /locus_tag="KKO15_003278"
     CDS             complement(11695..11853)
                     /locus_tag="KKO15_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBD6361962.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         3070 a         2917 c         2995 g         2955 t
ORIGIN      
        1 cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc acacggaccg
       61 tggaggccag tactgttcag cagattatca ggcgcaactg aaacggcata atctgcgtgg
      121 aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct tctttcattc
      181 gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa tgcgggcaac
      241 ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt ggtgtggcgg
      301 cctcaatccg gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc atattacgtg
      361 ggtaggatca atttgcccgc ttggcatagc gggcattttt tccaggtact tttgaatgag
      421 tactgatgaa taaatacatt gcagtggcgt gccacgtacc aaaacaccag ccctcattcg
      481 aaaccaccca ccgcacttct tccttgaaat ggcgttagtc atgaaatata gaccgccatc
      541 gagtacccct tgtaccctta actcttcctg atacatgaat gatgatttgg tggcccttgc
      601 tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaagg
      661 gccttaagta tgcaataaca atacttataa accacgcaat aaacatgatg atcttatgat
      721 gtaaataaca gatttttatg cgttcccatt caccttgggt cgtgattaca cgcgacataa
      781 aacccgacac cgcctccatt cgcaaagtcg atactcgcag tcaacaagca aatgttaata
      841 attagcacta tctatagtta tcatcgattc aatgatactt tgtaatgatt ttgtatctaa
      901 taatataact ttattacatt agctgaagag ttttcgcatc attatgatat ctgttacttt
      961 tcactccata aaaataaact tcatatagca atatattctt tcatagatct tattctgcta
     1021 atcattagtt tcgtatgcgc gatttttgac agttgcctct ccagaccaca tcgataatta
     1081 aaaaacagat ttaaacatta tccttttcca tatagatatt ggataaaaat aggacatctg
     1141 tttgcaatta ctttcacaac aattaaacat ttttatgttt tcgtatacat catattactc
     1201 taccattaga ggaacgttat tatgttttct ataaaaccag gacctggaaa tttacctatc
     1261 gacaacccca cattgttatc atggaacatt actgatgggg atctaaattc caaatttaat
     1321 acattagaat atctaaactg tataacaaat attattaatg cttgtggagt ttatcctcag
     1381 gatttaaaag acagagaaat tatatcaact tttcacgcag aaaaagtcat taatgatctg
     1441 ttaaaaaacg attataaaat ttccctttct ccagatacaa cttaccgaga gctgaataaa
     1501 gctgcacaga gtagcattac agcgccagac aggataggag aagggaaaac atgggtttat
     1561 caacgagata caatggttga aagaggtgat aacagtggtg ttcatcagta tggtccagct
     1621 gaacatttca ctcacattat atctgacaaa ccgtccccaa aagataagta tgttgcatat
     1681 gctattaaca ttcctgacta tgagctagca gccgatgtat ataatattaa cgtgacgtca
     1741 ccttccggac agcaagaaac atttaaaata ctaatcaatc cagaacatct acggcaaaca
     1801 cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat cacccccaaa
     1861 aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat cagggaaaat
     1921 atgtctgagt ttgcacgttc ccattatggg tatatacaaa tccctccagt gacaactttc
     1981 cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa
     2041 cccgaagatc tttgtaccat ccataatcca atgggggatt tgcaggattt tatcgcattg
     2101 gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt taactttatg
     2161 ggaatcgggg ggagtggtaa aaatgacctg gattatccct ctgctgatat acgagcgaag
     2221 atcagtaaag atatagaaag tggtatccct ggctattggc aaggtcaggt tttgattcca
     2281 ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga tatacatctc
     2341 actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg gaaaaacctc
     2401 catgcattaa aagaaaagag tttaaatgga atgcctaaaa caacccccaa aagtgaccag
     2461 gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt acgaggttta
     2521 cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat aaccccaccg
     2581 cttaaaaatt ataatgagcg attacacaat actaacttat ttaacccaaa atatcatgaa
     2641 aaaaccgtta tgaattacat ggaatatctg gtaacttgtc agttgaatga agaacaaatg
     2701 tcatcgctgc tttatgaaag agatgattta agcgccattg atttttcatt gctcatgaag
     2761 acgataaaag ccttttcatt cgggggagat ctcctaaccc ttgcatcaaa accgggttcc
     2821 acaatctcaa gcatcccatc agaaagacgg atattgatta acattaacca cgattttcct
     2881 aacaatggca atcttttcaa tgactttcta tttaaccatc aacaagatga acaattagca
     2941 atggcatata tggccgctct cccgttcagc aggcctttag tttactggga tggccaggta
     3001 ttaaaatcaa cgactgaaat taaaaattat gatgggtcca cgcgtgtcgg tggtgaggcg
     3061 tggcttaata aaggttgctc tacctatcag cagctctaca atgaattcca cgcattatat
     3121 atagataaag caggaatatg gagcgcattt gagggtgtat ttgcaactaa aaacgttctg
     3181 gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg actagttata
     3241 ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca acctggaata
     3301 tataaaaaca tggggagtaa tagcgttaac attattatta ataatacccg aaaaattatc
     3361 cccccttgta aagtatttac gcttagaggc ggatctctaa atatcaatat tcctaggcgt
     3421 agcgctcttc ttttagggaa aacaggagaa ccgccgaact atctctattt ataatttaat
     3481 gttatatctg ccccgataaa acggggcaga taatatgttt agtttactaa cggtcatttt
     3541 gtagtgaagc catttactgt tttttatcga ccagataatc tgttctctaa tgttaactcc
     3601 ccctaacctg ttgctttagt tattcatttc ctgtctcact ttgccttaat accctacgtt
     3661 aaatgttact aatttgttgc ttttgatcac aataagaaag caatatgtcg cttttgtgcg
     3721 catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat cgccatgtca
     3781 ccctacatct cataagagga tcgcttctga tgaatgcact gaccgccgta caaaataacg
     3841 ctgtcgattc aggctaggac tatagcggat tcactctcat cccgtcggcg caatccccgc
     3901 gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa caggttgccg
     3961 aatggcccgt gcaggcgctg gagtacaaat tgtttctgcg ttttcgggta ggcaaaattc
     4021 ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg
     4081 ctgaaggtgc gctgttgatc aatgcggtag gtatcgatga tgtggcgcag gcagatgaga
     4141 tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga
     4201 gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac agctatctgc
     4261 gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa gagatcaccg
     4321 attacgtgct gatgatgaaa atcgatgagc aaaacatgca gggtggaaat tcgttgctgc
     4381 tgcatctcga tgactgggaa catctggacc actatttccg ccatccactg gcgcgtcgcc
     4441 cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc catccagtgt
     4501 tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc gtccagccaa
     4561 aagattttga agaaggcgtg tggttgagcg agctttccga tgccattgaa accagcaaag
     4621 gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac ctgttctggc
     4681 tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg atgcgtcagc
     4741 gtggctattt cgcttacgcc actcaccact accagacgca tcagtaagcg caaaggaatt
     4801 gagcggatgt atgattttgt gattattggc ggcggcatca tcggcatgtc gaccgccatg
     4861 caactgattg atgtttatcc ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg
     4921 gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggtcta ttacacgccc
     4981 ggcagcctga aggcgcagtt ttgcctggcg gggaaccgtg ccaccaaagc cttttgcgat
     5041 caaaacggca ttcgctatga caactgcggc aagatgctgg tcgccacgtc tcagctcgaa
     5101 atggaacgga tgcgcgcctt atgggaacgc acagcggcga acggtatcga gcgcgagtgg
     5161 ataaacgccg tggaactgcg cgagcgcgaa ccgaatatca ccgggcttgg tggcattttt
     5221 gtgccgtcca gcggcattgt cagctaccgc gaagtgacgg cggcaatggc aaaaatcttc
     5281 caggccagag gcggcgagat tatttataac gccgaagtca gcgccctcag tgagcataaa
     5341 aacggcgtgg tgatacgcac ccgtcagggc ggtgaatatg aagcatcgac gctgattagc
     5401 tgttccgggc tgatggctga ccggctggtg aaaatgctcg gcctcgaacc gggctttatt
     5461 atctgcccgt tccgtggcga gtatttccgc cttgcgccgg agcataacca gattgttaac
     5521 cacctgattt accccattcc cgaccccgcg atgccatttt tgggcgtgca tctcacccgc
     5581 atgatcgacg gcagcgtgac cgtcgggcca aacgcggtgc tggctttcaa acgcgaaggc
     5641 tatcgcaagc gcgacttctc atttagtgac acgctggaga ttttgagctc gtcggggatt
     5701 cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc
     5761 aaaagcggct atctgcggct ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc
     5821 cagccctggc ccgccggtgt gcgggcgcag gcagtatcgc cggacggcaa gctgattgac
     5881 gattttctgt ttgtcaccac cccacgcacg atccacacct gcaatgcgcc ctccccggca
     5941 gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac gctgttggca
     6001 agccagagta accccggacg cacgctgcga gcggcacgta gtgtggatgc cttacacgcc
     6061 gcatttaatc aataaccttt gaaaacagga cgtaacaatg aaacttaacg acagtaactt
     6121 attccgccag caggcgttga ttaacgggga atggctggac gccaacaatg gtgaagtcat
     6181 cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtgccgaaaa tgggcgcaga
     6241 tgaaacccgc gccgctatcg acgccgccaa ccgcgccctg cccgcctggc gtgcgctcac
     6301 cgccaaagaa cgcgccacca ttctgcgcaa ctggttcaat ttgatgatgc agcatcagga
     6361 cgatttagcg cgcctgatga ccctcgaaca gggtaaaccg ctggctgaag cgaaaggcga
     6421 aatcagctac gccgcctcct tcattgagtg gtttgctgaa gaaggcaaac gtatttatgg
     6481 cgacaccatt cctggtcatc aggccgataa gcgcctgatt gttatcaagc agccgattgg
     6541 cgtcaccgcc gccatcacgc cgtggaactt tccggcggcg atgattaccc gtaaagccgg
     6601 tccggcgctg gcggcaggct gcaccatggt gctgaagccc gccagtcaga cgccgttctc
     6661 tgcgctggcg ctggcggaac tggcgattcg cgcgggcatt ccggctggcg tgtttaacgt
     6721 ggtcaccggt tcggcaggcg cggtcggtaa tgaactgacc agcaacccgc tggtacgcaa
     6781 actgtcgttt accggttaga ccgagattgg ccgccagtta atggaacagt gcgcgaaaga
     6841 catcaaaaaa gtgtcgctgg agctgggcgg caacgcgcag tttatcgtct ttgacgatgc
     6901 cgatctcgat aaagccgtgg aaggcgcgct ggcctcgaaa ttccgcaacg ccgggcaaac
     6961 ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg tatgaccgct ttgccgaaaa
     7021 attacagcag gcggtgagca aactgcacat cggcgacggg ctggagaaag gcgtcaccat
     7081 cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa gagcatattg ccgatgcgct
     7141 ggagaaaggt gcgcgtgtgg tttgcggcgg taaagcgcac gaacgcggcg gcaacttctt
     7201 ccagccaacc attctggtgg acgttccggc caacgccaaa gtgtcgaaag aagagacgtt
     7261 cggccccctc gccccactgt tccgttttaa agatgaagcc gatgtgattg cacaagccaa
     7321 tgacaccgag tttggtcttg ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg
     7381 cgtcggcgag gcgctggagt acggcatcgt cggcatcaat accggcatta tttccaatga
     7441 agtggcccca ttcggcggca tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg
     7501 catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg agaatgcgaa
     7561 ggaacagcaa taaagagtta atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc
     7621 aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc
     7681 gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac ctgcatccga
     7741 aggtggtggc cgcggtggaa gcgcagttga aaaaactgtc gcacacctgc ttccaggtgc
     7801 tggcttacga gccgtatctg gagctgtgcg agattatgaa tcagaaggtg ccgggcgatt
     7861 tcgccaagaa aacgctgctg gttacgaccg gttccgaagc ggtggaaaac gcggtaaaaa
     7921 tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt tagcggcgag tatcacgggc
     7981 gcacgcatta caccctggcg ctgaccggca aggtgaatcc gtactctgcg ggcatggggc
     8041 tgatgccggg tcatgtttat cgcgcgcttt atccttgccc gctgcacggc ataagcgagg
     8101 atgacgctat cgccagcatc caccggatct tcaaaaatga tgccgcgccg gaagatatcg
     8161 ccgccatcat gattgagccg gttcagggcg aaggcggttt ctacgccgcg tcgccagcct
     8221 ttatgcagcg tttacgcgct ctgtgtgacg agcacgggat catgctgatt gccgatgaag
     8281 tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat ggagcagatg ggcgtggcgc
     8341 aggatcttac cacctttgcg aaatcgatcg caggcggttt cccgctggcg ggcgtcaccg
     8401 ggcgcgcgga agtgatggac gctgttgcac cgggcggtct gggcggcacc tatgccggta
     8461 acccgattgc ctgcgtggct gcgctggaag tgttgaaggt gttcgagcag gaaaatctgc
     8521 tgcaaaaagc caacgatctg gggcagaagc taaaagacgg attgttggcg atagccgaaa
     8581 aacacccgga gatcggcgac gtacgcgggc tgggatcgat gattgccatc gagctgtttg
     8641 aagatggcga tcacagcaaa ccggacgcaa aactcaccgc cgagatcgtg gcacgcgccc
     8701 gcgataaggg cctgattctt ctctcctgcg gcccgtatta caacgtgctg cgcatccttg
     8761 taccactcac cattgaagac gctcagatcc gtcagggtct ggagattatc ggtcagtgtt
     8821 ttgatgaggc gaagcagtag caccgccccc atgcaggagg cgacgctgcg cgtcttgtcc
     8881 ggcctacgag aatccaagtc ggataaggcg tttacgccgc atccgacaat ctgtacgtga
     8941 acaggaataa atctatgtcg gccggataag gcggagccgc tctccggcaa aaagaatcaa
     9001 taacaattaa acgcgtgacc cgacgcggga aatgtcgggg cgctctccca agtgacacac
     9061 tttcgagagg attcaggatg gggcaatcat cgcaaccaca cgagttaggc ggcgggctga
     9121 aatcacgcca cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg
     9181 gttccagcgt cgccatcgcc gaagcggacc cggcggtatt actggcctat ctgttcgccg
     9241 gattactggt ggttatgatt atgcggatgc tggcggaaat ggcagttgcc acgcccgata
     9301 ccggttcgtt ttccacctat gccgataaag ccattggccg ctgggcgggc tataccatcg
     9361 gctggttgta ctggtggttt tgggtactgg ttatcccact ggaagccaac atcgccgcca
     9421 tgatcctgca ctcgtgggtt ccaggcattc ccatctggtt attttccctc gtcattaccc
     9481 tcgccttaac tggcagtaat ttattaagcg ttaaaaacta cggcgaattt gagttctggc
     9541 tggcgctgtg caaagtcatc gctatcctgg cctttatttt ccttggtgca gtcgcaatta
     9601 gcggttttta cccttatgcc gaagtgagcg ggatctcaag attgtgggat agcggcggct
     9661 ttatgcccaa cggtttcggt gcggtattaa gcgcgatgtt gatcaccatg ttctcgttta
     9721 tgggcgcaga aattgtcacc attgccgccg cggaatccga catgccggaa aaacatattg
     9781 tccgcgccac caactcggtt atctggcgta tttctatctt ctatttgtgc tctatttttg
     9841 tcgtcgtggc attaataccg tggaatatgc cggggctgaa agccgttgga tcttatcgct
     9901 cggttctgga attgctcaat attccccatg cgaaattaat catggactgt gtgatattac
     9961 tttccgtaac cagttgcctg aactcggccc tgtataccgc ctcaaggatg ctctactcct
    10021 taagccgtcg cggtgatgct cccgcggtaa tgggtaaaat caaccgcagt aaaaccccgt
    10081 acgtggcggt gttactctcc accggcgcag cattcttaac ggtggtggtg aactattacg
    10141 cgcctgcgaa ggtatttaaa tttctgatcg acagctccgg cgctatcgcc ctgctggttt
    10201 atttagtcat cgccgtttca cagttgcgga tgcgcaaaat tctgcgagca gaaggaagcg
    10261 aaattcgctt gcgaatgtgg cttgatccct ggctcacctg gctggtcatc ggctttatta
    10321 cctttgtgtt ggtagtgatg ctattccgtc cggcgcaaca gttagaagtg atctccaccg
    10381 gcttattagc gatagggatt atctgtaccg tgccaattat ggctcgctgg aaaaagctgg
    10441 tattgtggca aaaaacaccc gttcataata cgcgctgata atgatcagga gtcacaccat
    10501 gaccattacg tctctggatg gctatcgctg gctgaagaac gatattattc gcggtaattt
    10561 tcaaccggat gaaaaattac ggatgagttt actgacatcg cgttatgcac ttggcgttgg
    10621 gccgttgcag gaagcacttt ctcaactggt ggcggaacgg ctggtcacgg tggtgaatca
    10681 aaaagggtat cgggtggcgt ctatgtcaga gcaggagctg ctcgatattt tcgacgcccg
    10741 cgccaatatg gaagcgatgc tggtcagtct ggcgattgcc cgcggtggcg atgaatggga
    10801 ggcagacgtt ctcgcaaaag cgcatctgct cagtaagctg gaagcctgtg acgccagcga
    10861 gaaaatgctt gatgagtggg atctgcgtca tcaggcgttt catacagcaa ttgtggcggg
    10921 ctgtggttct cactatttgc tgcaaatgcg tgaacggttg tttgatctgg cggcgcgtta
    10981 tcgatttatc tggctgcggc gaacggtgct ttcggtggaa atgctggagg ataaacacga
    11041 tcagcaccag accctgactg cggcggtact gccgcgagat acagcgcgcg ccagtgagtt
    11101 aatgcgccag catttactga cgccaattcc cattatccag caggcgatgg ctggcaatta
    11161 actactcttc cggaatacgc aacacttgcc ccggataaat tttatccggg ctttttagca
    11221 tcggtttatt cgcttcgaag attttattgt acagattagc gttaccgtag acctgtttgg
    11281 aaatggcact cagagtgtcg ccagacttaa cggtataaaa ctggctggca gtggctggtg
    11341 tcgccgtttt cacctgatca tcaacactgg caataccgga aatattcccc accgcaacaa
    11401 ggattttctc cttcgcctcc tgactcaggc cgtcaccagt gaccgtcgct ttgccgtcgg
    11461 caatttgaat gttcacttta tcggcatcag gtataccggt tttgctcaga tgctcctgca
    11521 ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac cgcatcccag agtttttctc
    11581 cggcatcttt aacaaaattg aacagaccca tagctacctc attcgttaac ggaaacactc
    11641 agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg gggtgagcac aggtttaatc
    11701 gcgggtttgc acccagaacg cgtgaatcaa accggggata tacccaagca gtgtcagcag
    11761 aatattaata atgaacgccc aaccgaaccc tttaccgagc agcacgccga tcggcggcag
    11821 aatgatggtg atgacgattc tccagaaacc catatgtact ccctataaga aaattactca
    11881 ttgtttaaaa agagatttta tctcttaagc gtagtaattt tagcggagcc tgccagt
//
LOCUS       DAECOD010000113        11923 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.113, whole genome shotgun sequence.
ACCESSION   DAECOD010000113 DAECOD010000000
VERSION     DAECOD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.113"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..2340)
                     /locus_tag="KKO15_003279"
     CDS             complement(<1..2340)
                     /locus_tag="KKO15_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD6361963.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEEMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="KKO15_003280"
     CDS             complement(2274..2897)
                     /locus_tag="KKO15_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBD6361964.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="KKO15_003281"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="KKO15_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KKO15_003282"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KKO15_003282"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBD6361965.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /locus_tag="KKO15_003283"
     CDS             7505..8227
                     /locus_tag="KKO15_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBD6361966.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..10289)
                     /gene="yfaL"
                     /locus_tag="KKO15_003284"
     CDS             complement(8355..10289)
                     /gene="yfaL"
                     /locus_tag="KKO15_003284"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBD6361967.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10299..11894)
                     /locus_tag="KKO15_003285"
     CDS             complement(10299..11894)
                     /locus_tag="KKO15_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBD6361968.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2756 a         3307 c         2946 g         2914 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccgtc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatctcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact caggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccggcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cgg
//
LOCUS       DAECOD010000114        11710 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.114, whole genome shotgun sequence.
ACCESSION   DAECOD010000114 DAECOD010000000
VERSION     DAECOD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.114"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            57..851
                     /locus_tag="KKO15_003286"
     CDS             57..851
                     /locus_tag="KKO15_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD6361969.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="KKO15_003287"
     CDS             873..2282
                     /locus_tag="KKO15_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361970.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVVAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="KKO15_003288"
     CDS             2260..3039
                     /locus_tag="KKO15_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBD6361971.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="KKO15_003289"
     CDS             3036..3896
                     /locus_tag="KKO15_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD6361972.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="KKO15_003290"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="KKO15_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="KKO15_003291"
     CDS             complement(4636..4896)
                     /locus_tag="KKO15_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD6361973.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="KKO15_003292"
     CDS             complement(4887..6158)
                     /locus_tag="KKO15_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6361974.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="KKO15_003293"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="KKO15_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBD6361975.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="KKO15_003294"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="KKO15_003294"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBD6361976.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTQAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="KKO15_003295"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="KKO15_003295"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBD6361977.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="KKO15_003296"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="KKO15_003296"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBD6361978.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="KKO15_003297"
     CDS             11500..11652
                     /locus_tag="KKO15_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD6361979.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2810 a         2989 c         3234 g         2677 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggcgtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggtggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggctg ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc agccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacactcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc caagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cttggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc
//
LOCUS       DAECOD010000115        11649 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.115, whole genome shotgun sequence.
ACCESSION   DAECOD010000115 DAECOD010000000
VERSION     DAECOD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.115"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            597..794
                     /locus_tag="KKO15_003298"
     CDS             597..794
                     /locus_tag="KKO15_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361980.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KKO15_003299"
     CDS             complement(744..1937)
                     /locus_tag="KKO15_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6361981.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KKO15_003300"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KKO15_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBD6361982.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KKO15_003301"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KKO15_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBD6361983.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHNRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KKO15_003302"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KKO15_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBD6361984.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KKO15_003303"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KKO15_003303"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBD6361985.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KKO15_003304"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KKO15_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBD6361986.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KKO15_003305"
     CDS             complement(10573..10716)
                     /locus_tag="KKO15_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361987.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11649
                     /locus_tag="KKO15_003306"
     CDS             10966..>11649
                     /locus_tag="KKO15_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6361988.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLF"
BASE COUNT         2955 a         2895 c         2987 g         2812 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataatcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctattt
//
LOCUS       DAECOD010000116        11456 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.116, whole genome shotgun sequence.
ACCESSION   DAECOD010000116 DAECOD010000000
VERSION     DAECOD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.116"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(58..1440)
                     /gene="cusC"
                     /locus_tag="KKO15_003307"
     CDS             complement(58..1440)
                     /gene="cusC"
                     /locus_tag="KKO15_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBD6361989.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRAWALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            1597..2280
                     /gene="cusR"
                     /locus_tag="KKO15_003308"
     CDS             1597..2280
                     /gene="cusR"
                     /locus_tag="KKO15_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBD6361990.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2270..3712
                     /gene="cusS"
                     /locus_tag="KKO15_003309"
     CDS             2270..3712
                     /gene="cusS"
                     /locus_tag="KKO15_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBD6361991.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3862..6099
                     /gene="nrfB"
                     /locus_tag="KKO15_003310"
     CDS             3862..6099
                     /gene="nrfB"
                     /locus_tag="KKO15_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBD6361992.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            6086..9054
                     /gene="nfrA"
                     /locus_tag="KKO15_003311"
                     /pseudo
     CDS             6086..9054
                     /gene="nfrA"
                     /locus_tag="KKO15_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            9055..9945
                     /locus_tag="KKO15_003312"
     CDS             9055..9945
                     /locus_tag="KKO15_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBD6361993.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10128..10889
                     /gene="envY"
                     /locus_tag="KKO15_003313"
     CDS             10128..10889
                     /gene="envY"
                     /locus_tag="KKO15_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBD6361994.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10935..11011)
                     /locus_tag="KKO15_003314"
     tRNA            complement(10935..11011)
                     /locus_tag="KKO15_003314"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10975..10977),aa:Arg,seq:tct)
     gene            11254..>11456
                     /locus_tag="KKO15_003315"
     CDS             11254..>11456
                     /locus_tag="KKO15_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBD6361995.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         2798 a         2986 c         2931 g         2741 t
ORIGIN      
        1 gactgaacat tgcgacttgc agtgcttttt tcatttattc tctcctggag ttaaaaatta
       61 ttgctgccaa ccgcccccta aggcggtata caaataaatt tcgttaacct gacgggcata
      121 attcaggtca agtaaagttt gtcgggttgc aaataaagaa cgctcggcat ccagcacttc
      181 cagataactt accgcgccgt gctgatataa tgcccacgcc cgttgcaggg taatttgcag
      241 cgacgccaga taacgctgct gggcgctgat ttgatcgtcc aggctttgac gtaatgccag
      301 cgcatctgcc acctctttaa aggcgttctg gattttctgt tcataattca ccaccgactg
      361 ctgctggcga atttcggcga tatccagatt ggcctggttg cgtccggcat taaaaatggg
      421 gatctcaatt ttaggaataa aattccacat cccgctgcta gcgttaaata atgacgacag
      481 atcgctgctg gcggttgata ttccgctggt caggctgata gacgggaaaa atgccgcacg
      541 cgcagcacca atattagcat tagccgccat taacgcgtgt tcagcttcca taatatccgg
      601 gcgctgcaat aagatttgcg acgacaagcc cgccggtaat ttaacgcttt gcaggctgcc
      661 gctgtttact gtctgcgctt gcggtagctt gccgtagctt cccaataaca gttgcaatgc
      721 attattcgcc tgcgccagtt ccccctgacg tttagcgatg tcactgcggg tactttctat
      781 caccccgcga gcctgttcca gcgccagaac attgctgcta ccggtcaaca gttgtttttc
      841 gacaaacgca tatgactgct gataattacg cagcgtttct tcggctattt gcaattgcgc
      901 atacgccagc tgctgattga aatagctttg cgcgacatta gaaaccagca aaatatgcac
      961 cgcgcgctgg gcttcctcag tggctaaata attttggcgc tcggcttcgc tcatgttctt
     1021 taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg ccagtcgaga actcccgcgt
     1081 cgtggctgaa tcgcctttaa gattgccgct ccagctgccg ctgccctcgc cattgagctg
     1141 tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt gcctcctgca ctttcagcgt
     1201 cgccatgcgc aaatcccggt tattcaccag cgcctcgcta atcagcgtct tcacctgatt
     1261 atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta tccgctgcgt taaccaggcc
     1321 gttctggctg agtgagaact gctgcggtac gggcattacc ggacgctgat aatccggtgc
     1381 cagtgaacaa ccagttagcg caagggccac acaaaatggc agaagtttac aaggagacat
     1441 aggctcataa tttctggtga ttttatgtcg ccaactttac tcgccaggct ctgattttcc
     1501 ggtgacagga aaatgacaaa attgtcattt tgccaataag cgattgccat ctgatcccgc
     1561 tactctagaa ttgcccgggc aacatgcgga ggaaatatga aactgttgat tgtcgaagat
     1621 gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg aagccggttt tgtggtcgat
     1681 ttggccgaca acgggctgaa tggctaccat ctggcgatga ccggtgatta tgacctgata
     1741 atcctcgata ttatgctgcc agacgtgaac ggctgggata tcgtgcgcat gttgcgctcc
     1801 gccaataaag ggatgccgat tctgttgctt accgcgcttg gcaccattga acatcgcgtc
     1861 aaggggctgg agttgggggc agatgactac ctggtgaaac cgttcgcttt tgctgaactg
     1921 ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg tgattatcga aagtcagttt
     1981 caggttgccg atctgatggt cgatctcgtc agccgcaaag tcaccctcag cggcacgcgc
     2041 atcactctga ccagtaaaga atttactctg ctggagtttt ttctccgcca tcagggcgaa
     2101 gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca tgaatttcga cagcgacact
     2161 aacgccattg atgtggcggt gaagcggcta cgcggcaaaa tcgacaacga ctttgagccg
     2221 aagctgattc aaaccgtgcg cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa
     2281 gccatttcag cgcccgtttt cgctggcaac ccgcctgacc ttttttatca gcctggccac
     2341 catcgcggcg tttttcgcct ttgcatggat cataatccac tcagtaaaag tgcattttgc
     2401 cgagcaggac attaatgatt taaaagagat tagcgccacc cttgaacggg tactaaatca
     2461 ccctgacgaa acgcaagccc gacgcttaat gacgctggaa gatatcgtca gtggttattc
     2521 caacgtgttg atttccctgg cagatagcca cggcaaaacg gtgtatcact cccccggtgc
     2581 gcctgatatc cgcgagtttg cacgtgatgc catacccgat aaagacgcgc ggggtggcga
     2641 ggtgtatctc ctttccggcc cgacgattat gatgccaggc cacggtcacg ggcatatgga
     2701 acacagcaac tggcggatga ttaacttgcc ggttggcccg ttggtggacg gcaaaccgat
     2761 ttatacgctc tacatcgcgc tgtcgatcga ttttcatctt cattacataa atgatttgat
     2821 gaataaactt attatgaccg catcggtaat cagcatcctg atcgtcttta tcgtgctgtt
     2881 ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc cgtcaaatcc agaatatcac
     2941 ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg ccgattgagc tggaacagct
     3001 ggtactgtcg ttcaaccata tgatcgagcg tattgaggat gtctttactc gccagtccaa
     3061 tttctcagcg gatatcgccc acgaaattcg cacaccaatt actaatctca ttacgcaaac
     3121 ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg gaagatgtgc tctactctaa
     3181 tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat atgctgtttc tcgctcaggc
     3241 cgataacaac cagctaatcc ccgaaaagaa aatgctcaac ctggcggatg aagtcggcaa
     3301 agtgttcgat tttttcgagg cgctagcgga agatcgcggc gtggagttgc ggtttgttgg
     3361 cgacaaacgt caggttgcgg gcgatccgtt gatgctgcgt cgggcgctaa gcaacctgct
     3421 ttctaatgcc cttcgttata cgccaaccag agagacaatt gtagtacgct gtcagacggt
     3481 cgatcaccag gtgcaagttt ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt
     3541 accgcgattg tttgaccgtt tctatcgcgt tgacccctcc cgccagcgaa aaggtgaagg
     3601 tagcggtatt gggctggcga tagtgaaatc gattgttgtc gcgcataaag gcacggttgc
     3661 ggtaacgtca gatgcgcggg ggacaaggtt tgttatcaca ttacccgctt aattattcag
     3721 caaacggcaa cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacatattaa
     3781 tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca
     3841 gcgttaatgt aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctac
     3901 ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat
     3961 ttttttattg atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc
     4021 cgctacccgc gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt
     4081 atggttccgg cgtggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc
     4141 acgctcgact acgaaaacta tcatatcttt gttggcacct accccaacga ccccgatact
     4201 caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg tgcataaggt agtctgcgcg
     4261 cgtcctggtc ccaccagcaa agccgactgc ctgaacaacg tgctggacgc catcacccag
     4321 tttgaacgta gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg
     4381 atttcaccga tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag
     4441 atcccagtgt atccgtttga acgcgaatgg acgcacttca ccagcatgac ttacattgat
     4501 gagttttcag aactgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg
     4561 cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg tgaccgcact gttagctgac
     4621 ggtaacggta ttgctttcga cgtgcagagt cttactgaag attacgacat tggcttccgc
     4681 ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa
     4741 gaacgcgagc agcgtaaatt tttacagcac gcacggacgt caaacatgat ctgcgtgcgc
     4801 gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc
     4861 attgttttcc agggctttaa aacccataaa tggacctcca gcctgacgct gaactacttt
     4921 ctctggcgcg accgcaaagg ggcaatcagt aactttgtca gcttcctcgc gatgctggtg
     4981 atgatccagc ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc
     5041 ctttctattt ttagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggcttg
     5101 atggttaacc gcatcgtgca gcgagtgatt ttcgtcactg gctactacgg cctcacgcag
     5161 ggcctgcttt ccgtcctgcg tcttttctgg ggcaacctga ttaactttat ggctaactgg
     5221 cgcgtgctga aacaggttct tcagcacggc gatccgcgtc gcgtcgcgtg ggataaaaca
     5281 acgcatgact tccccagcgt cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt
     5341 ctgctggaaa atcaggtcat cactgaagaa caactcgata cagcactgcg taatcgcgtc
     5401 gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgagcagctg
     5461 gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc
     5521 ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc tgcattatgc agtactgccg
     5581 ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag atggtattga cccggtttcg
     5641 ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt tctgcgagga
     5701 caaattgtca cagggttacg ccactggtat gcacgccgac gcggtcacga tccgcgggca
     5761 atgttgtaca atgcggttca gcatcagtgg ctcacggaac agcagaccgg tgaaatctgg
     5821 cggcaatatg tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt
     5881 aatcgttcag caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc
     5941 aagtttttgg tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt
     6001 caacgcgaat tacaagtttc gatgcaatca ctattactca aagcaggttt aaacacagaa
     6061 caggttgcgc aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg
     6121 gtctggttta ctgctgacgt ctttattgag taccagcgca ctcgcagaca atatcggcac
     6181 cagcgcagaa gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga
     6241 taaggcgctg aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata
     6301 tatacaccaa caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg
     6361 tcattttggt catgatgacc gggcgcggct gttgcttgag gatcaactga aacgtcaccc
     6421 aggagatgcc cgacttgagc gcagtctggc ggctattcca gttgaagtga aaagcgttac
     6481 gactgttgaa gaactgcttg cccagcaaaa agcgggcgac gctgcgccga ccctgcgttg
     6541 tcgcagtgaa gtcgggcaga atgccctgcg gctggcacaa ttacctgtcg ccagagcgca
     6601 actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct
     6661 gcaacgggca atctacctga aacaatgatc ccaggcagat acgctataca atgaagcacg
     6721 ccagcagaac acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg
     6781 gcagctggac gatcggatcc tggcactgca atcacagggg atcttcaccg atcctcagtc
     6841 atatattact tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc tccagcatta
     6901 tctcattgaa aataagccgc tgtttaccac gggcgcacaa gagaaaagtt ggctctatct
     6961 gttatctaaa tacagcgcca accccgttca ggcattggcg aattatacgg tgcagtttgc
     7021 cgataaccgc cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta
     7081 cgacgcagcg caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta
     7141 tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg cgtctggcac gattgctgta
     7201 tcagcaagaa ccgacaaatc ttccccgcct ggatcaactg acctggcaac tgatgcagaa
     7261 cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc
     7321 gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg ctggaaagtc atccttacct
     7381 agcaacgccg gcgaaggtgg cgattttatc gaaaccctta ccgctggcgg agcaacgtca
     7441 gtggcaaagt cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg
     7501 cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt ctggcaaagt gttatcggga
     7561 cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc gaacaacgac aaccgagcgc
     7621 ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt gaggactacg ccaccgcact
     7681 ggcggcctgg cagaaaatca gtcttcacga catgagcaat gatgatctgc tcgccgccgc
     7741 caacaccgct caggcagcgg gaaatggcgc ggctcgcgat cgctggcttc aacaggcaga
     7801 acaacgtgag ctgggaaaca atgctcaata ctggtggctg catgcgcgac gttatatccc
     7861 agctcagcca gaactcgcac tgaacgatct cacgcgctca atcaatattg ccccttctgc
     7921 caactcttac gttgcgcggg cgacaattta tcgccaacgt cataatgttc cggccgcggt
     7981 gagtgatttg cgcgccgcgc tggaactgga accaaataat agcaacaccc aggcagcgct
     8041 tggttacgcc ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc
     8101 gcataaaggg cttccggacg atccggcact gatccgacaa ctggcctacg tgaaccagcg
     8161 tctggatgac atgcctgcga cgcagcacta cgcccggctc gtgattgatg acattgataa
     8221 tcaggcataa ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg
     8281 catgaggagg tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc
     8341 ggcgcaatga gtaccgctaa caataatgtc ggcggtgcag cgcctgggaa aagctatcgt
     8401 agctacggac aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac
     8461 ctgctctcag tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg
     8521 gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt
     8581 ttcctcgccg tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg
     8641 ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac
     8701 ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat
     8761 attcaggcgt ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag
     8821 actattgagc cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc
     8881 gcgcagcttg gcggtgtcgg ggtccgctgg aatatttgga ccggcgagac gcactacgac
     8941 gcctggccgc acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat
     9001 caacgtaacg gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt
     9061 aagttcattt tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa
     9121 ggtattatct ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg
     9181 ctgatgagtc agttacgttt tcaaggcttc gataccctgg ttttgcaatg gacccgttac
     9241 ggcgatgcat ttacccagcc agaacagcgc acgttattgt ttaagcgtgc cgcagctgcg
     9301 caacagactg gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac
     9361 cagaaacagt cgtctgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc
     9421 cagcaagcca gattatggag cgccgcgcct ggcataacgc cggatggctg gtacatcagc
     9481 gcggaaattg acgacctgaa ctggcgcagc gaagccgccc gtcaaccttt gctaacatgg
     9541 ttaaacaacg cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt
     9601 tttttcgccg gaaacatgtc gcccgatggc tatcgccaac tgctggaaca agttaaagca
     9661 accggagtta atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag
     9721 cgtgaacgtt atctacaggc cagcgccgat tgccaaagct ccgccccagc aagcggcatt
     9781 gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac
     9841 gcagaaattg cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacat gctctatttc
     9901 tctctgcgct atttgcccgt cgcgcacggc attctcgagt attaaatccc ctccaggtaa
     9961 gtcgggtacg acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc
    10021 taaaaagcat gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg
    10081 aagcatagat cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca
    10141 gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc
    10201 atgctacgtt tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca
    10261 ttgaactctc aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg
    10321 attatctgtt gtttttaaat aagaaattaa cctgtgtaaa gccctggtcg cggctggcaa
    10381 ccccggttat cgcttgtcat agccgtacac cggaagtgtt ccggctagcc accaaccaca
    10441 gcaagcagca atccagcaaa ccctgcgaag cggagttgac gcgcgcatta ctttttaccg
    10501 tattgtctaa ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca
    10561 gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc
    10621 tgcgaattgt cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa
    10681 acgaaaatac cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga
    10741 tgttattgat ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca
    10801 cgtcgtactt tatctctgtt tttaaggcgt tttacagcct gacaccgttg aattatctcg
    10861 ccaaacagcg acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat
    10921 aaatgaagaa gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagg
    10981 tcgttgctct atccaactga gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt
    11041 gaattatacg gtcaaccctt gctgagtcaa tggcttttga tctggttgct gaacaagtga
    11101 acgaccgcgt ctgattttct gatttatttc gctatagcgg caaacaaacg cacaccgctg
    11161 cgcgtctgaa tcaagaaaac ccgtattttc atgtatcaaa gtacaattta ccgccctaac
    11221 ggaaaattgt ccgctcctat gagactggta actatgaaac caacgtcggt gatcattatg
    11281 gatactcatc ctatcatcag aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg
    11341 cagattgtcc tgaaaacgga tgattaccgc ataaccatcg attatctccg aacccgtcct
    11401 gttgatttaa tcattatgga tatagacttg cccggaacag acggttttac cttcct
//
LOCUS       DAECOD010000117        11289 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.117, whole genome shotgun sequence.
ACCESSION   DAECOD010000117 DAECOD010000000
VERSION     DAECOD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.117"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..458)
                     /gene="intS"
                     /locus_tag="KKO15_003316"
     CDS             complement(<1..458)
                     /gene="intS"
                     /locus_tag="KKO15_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBD6361996.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(620..694)
                     /locus_tag="KKO15_003317"
     tRNA            complement(620..694)
                     /locus_tag="KKO15_003317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Arg,seq:cct)
     gene            complement(768..1700)
                     /locus_tag="KKO15_003318"
     CDS             complement(768..1700)
                     /locus_tag="KKO15_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBD6361997.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1994..2749
                     /gene="mlaA"
                     /locus_tag="KKO15_003319"
     CDS             1994..2749
                     /gene="mlaA"
                     /locus_tag="KKO15_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBD6361998.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGVNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2931..3989)
                     /locus_tag="KKO15_003320"
     CDS             complement(2931..3989)
                     /locus_tag="KKO15_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6361999.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="KKO15_003321"
     CDS             complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="KKO15_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBD6362000.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6067..6351
                     /locus_tag="KKO15_003322"
     CDS             6067..6351
                     /locus_tag="KKO15_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBD6362001.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6531..7841
                     /gene="fadI"
                     /locus_tag="KKO15_003323"
     CDS             6531..7841
                     /gene="fadI"
                     /locus_tag="KKO15_003323"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBD6362002.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7841..9985
                     /gene="fadJ"
                     /locus_tag="KKO15_003324"
     CDS             7841..9985
                     /gene="fadJ"
                     /locus_tag="KKO15_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBD6362003.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10188..10673
                     /gene="sixA"
                     /locus_tag="KKO15_003325"
     CDS             10188..10673
                     /gene="sixA"
                     /locus_tag="KKO15_003325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBD6362004.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2825 a         2581 c         2780 g         3103 t
ORIGIN      
        1 gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga
       61 atctcaaggc cgccgatgat gggtaaaatg tcgtcatcaa acatttttgc aagttcagtc
      121 gcatacccta ctgaccagac ttgcttcttg tgttcgtacc attccttgta aatcgcacta
      181 aatgaattgt tgttagacga agcctttttc gcctttaccg gatcgatgcc agccgagatg
      241 tctttcctcg cagtccatgc tttatccctt gcctcctgca aagtcataag cggatatttt
      301 ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc
      361 ccggatacag ggacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct
      421 ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa
      481 attattttat ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc
      541 atgacgaact gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg
      601 atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag
      661 gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcgagtatac ccgctaatgg
      721 actgcggggt aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgctgggttt
      781 ttatcatttt tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc
      841 ttgttgctca tgtcgttacg aatctgtgca tgactcatta acgcgaagat aaaggtgccg
      901 ccgcagatgt tccccgctaa agtaggtagt gcgaagggcc agatgaaatc gctccagtgc
      961 agcgtaccgt taaacaccag atagaggatt tcaacagaac cgaccacgat atgggtggtg
     1021 tcacccaggg caataagcca ggtcatcaat ataatcacca caatctttgc cgcacctgct
     1081 gcaggaaaca tccataccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac
     1141 atctcgctgg gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt
     1201 tcttcattga agataggcat atattcaaat gcccacgcag caataccagt cccgagaata
     1261 ttacccagca gcacgactcc ccataaccgc ataagtaagc caacgttgct cattgtcggt
     1321 ttttgcatga cgggtagtac cgcagtcacg gtgttttcgg taaataattg ctggcgggcc
     1381 attatgacga taataaaacc aaaggtataa ccgagattct ccagcaagaa gctgcccggc
     1441 acaccttcca gttcggcatg aaatatccct tttgccagta acgaagcgcc catcgacaga
     1501 cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc gttctagctc tttttcacca
     1561 tcctggcgga tatgctcatg aattgccatc gctcgggagg ggagtcggtc ttcatctatt
     1621 tctatttttt tgccgcgctc tttttcttcg ctctcaactt caatttcgtc gctgtgttga
     1681 tcaattttgt cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag
     1741 cggctttttt gctcgcaact ggcgggagtt actctgaaaa tgtagaaaag gctgcgtttg
     1801 cctttttctg tttctataga atcaagtagc ctacagggcg gcgattacca ggctatgatc
     1861 aaatcagcaa atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct
     1921 aaacaggcgg atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa
     1981 cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt
     2041 ggggtgtgcg agttccggta cagatcagca agggcgttct gacccgttag aaggggtcaa
     2101 ccgcaccatg tacaacttca acttcaacgt attagacccg tatattgttc gaccggtcgc
     2161 tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaat ggtttgagca actttactgg
     2221 caaccttgaa gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg
     2281 gatggtccac tttacccgct ttttcctgaa caccattttg gggatgggcg gctttattga
     2341 tgttgcaggg atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac
     2401 gcttggtcat tatggcgtgg gttatgggcc ttacgttcag ttaccgttct acggtagctt
     2461 cacgctgcgt gatgacggtg gtgatatggc ggatgctctt tacccggttc tttcctggct
     2521 gacctggccg atgtctgtgg gtaaatggac gcttgaaggg atcgaaaccc gcgctcagct
     2581 gctggattcc gatggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc
     2641 gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc
     2701 gaacgcgcaa gcgattcagg atgatttaaa agatatcgat tctgaataag aaacaaataa
     2761 aaaaggtgag tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa
     2821 ggagataaac cgacaatgac ccggacgtaa tgatgtaaaa atactgatgt ttgtcactga
     2881 ctatttccga tattcaggcg ttgaaaaatc aaaaaactgg aaaaataatt ttaaatatca
     2941 tcaaaattaa cacctagtgc agatagttga ttaatttcat tttgatattt cgtctggcga
     3001 ttttgaagtc ttttttcaac tttatcttcg agattcttaa agtctttgta tttattttca
     3061 ggatggtcaa cgtcaacttt gttagcgaat aaagtagaaa ttatttcttt tacattatcg
     3121 cgtctaaatt ttgcatcgtc cacttgaaaa ttatatctta ttgctcgacc taaacgttta
     3181 aaaacttcgt taggttctct cagtctttcc ccgttgagct cgtttacaac ctgatgatcg
     3241 gaaccagttg caaatgattc tgccatgata gctttactgc tttcaaacca cgctgaagca
     3301 ctcctggcgt tttcactaca atattgggtg agactatcac cattaatacg aattcctctt
     3361 tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt tccgttttta
     3421 tttttaatta caatagcgag agagtcgcct tttttatctt taccgtacca ggtaaataag
     3481 tccgttggtt cgcttttccc gctctcatca aattccgtta ccgtatgtct gagtttgtgt
     3541 gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa tgcgctgaag
     3601 atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt aatgatagta
     3661 cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg gttgtcgata
     3721 ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac tgaggaatta
     3781 ttaatattag atctataagg atcgtcgttt actcgtatgg cgttttttat aagctcaagt
     3841 agatgtttaa tacaggtgct tattccttcc ttaccattaa aaaggttttt gaactcttgt
     3901 actaatccat taatttcatc attgttattt tcattgatgg attctgggta agctgcgctg
     3961 gtattgttga ttgaaataga tggtagcatg attttattcc atgttgattg atttgttaca
     4021 aatatatatt acctaatgaa atagactatc aaaaaacgct catttattta gaacctatct
     4081 ttaatttgag tgtataagcg cacaatagcc aggggggttg agtatcctgg aaatgcagaa
     4141 aagtaaactc tttgagaagg acgagcagaa cgcttgaaaa tatgcatttc ccttcaggtt
     4201 tgtctgtgga tactacttac tgatttgaaa gggcaagctg ccatcaggct gtttgcaaaa
     4261 ctcaggaggc aagcaatgag gaaactactt tcggtgaagt gggggagtta aagtcacctg
     4321 catagcaggt gactttatcc aggcgaacgc gttatcagaa cgcgtagtta aagttagtac
     4381 cgaacagcca ggctttacct tcagactcga actggtatgg gccttcgtta attttcacgc
     4441 tctgaccgtg catataagaa acaccaacgt cgactgaagc atctttatta aatgcgtaag
     4501 tcgtacctgc actcagccag aaacggtcct ggtccggaat ggagatagaa cgattctgag
     4561 ccggaactgg gctgtcatca aaggcgatac cggtacggaa ggtccagtta tcatcgtagt
     4621 aataagtggt acccaacgcg atgcggtaag catctttaaa gccttcatgt ttctggaaca
     4681 gcgtgtcgcc actggttgag gtcgctttca gctgctggaa ctgactccag ctggtgtaag
     4741 ccaggctata gtgaatcgcc cactgtggat caacacggtt ataacctgac acttcccaca
     4801 tttcaggcag gttcagcgtc agataacccg attgcgttgc gccacctgtc gcggtaggaa
     4861 ttggcaaacc gtagttatta aacgcacgat taagatcgct gctgtagtta cctttgaagt
     4921 caattttcac ttcagaacgg taggtcagtg catagcggtt atttttatcc agttcataca
     4981 ggattccggc gttccagcca aagccccact ggttaccgtt cagatgagcg attttggtat
     5041 tactgtcgat accgttggcg gtagctgcca atgcttgccc ttgaggagtt tgcccggcag
     5101 gagattgcat aatctgacca gcaaccaact gccccagatc gcctgcgaaa cgttcaattt
     5161 tcgcgcgagc gtagatggcg ttgaaaccaa gaccaaagct ccatgcatta tttaagcgat
     5221 acgcaccgct taagttcagg ttcatggttt caaggtcagt tgtacccccg acagagccgc
     5281 ctgcataagt atcattaaac tctgttgcca gaccatagtt agaggtaata gaagcgcccc
     5341 aaccaaattg gtcgttaatc ggtgcaacaa agtgcatgtt cggaacccat gccgtaggcg
     5401 cgatgttatc ggctttcagg ctacgaccag acggagacgt tccgctgata ttcacatccg
     5461 ggtcaatata aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta atcaatgcgg
     5521 ggttacggct aacgttacct gcatcatcgg caattgcgcc ttcccctgaa taagcccggc
     5581 ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc tgggtggaga
     5641 taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg ctcatgacca
     5701 taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg agcgcgaagt
     5761 gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg tcggaccagc
     5821 tagaatgttg caagtatgtt tccatctttt ttcaaatatg atctaagaaa cgcgatccag
     5881 ctggcaaaat ttggaggttg cgcactcgcg gattcgggtg aattttgttt tagatcattt
     5941 ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga gcagcacttt
     6001 ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat acagaggaaa
     6061 tcaacgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat ggatgttggc
     6121 accattatgg acaactccga ctgcaccgcg tcttacagcc gcgtgtttgc aaaccgcgca
     6181 gaagcagaac aaacgctggc ggcgctgact gaaaaatccc gtagcgtgga atccgaaccg
     6241 tgcaaaatct ccccgacttt cactgaagag tctgacggtg tgcgcctgga tatcgatttc
     6301 acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta attcctctcg
     6361 ggtacgtacg cccctgtcag ttgctggcag gggcgttttc ttttctttct tctgtcttca
     6421 ctgtgtcata gctcccactt tcctttttct ccgcttggct aaatgtaaaa aaatggttaa
     6481 gactatgatc aggtcagacc actttattta tttttttaca ggggagtgtt atgggtcagg
     6541 ttttaccact ggttacccgc cagggcgatc gtatcgccat tgttagcggt ttacgtacgc
     6601 cttttgcccg ccaggcgacg gcttttcatg gcattcccac ggttgattta gggaagatgg
     6661 tggtaggcga actgctggca cgcagcgaga tccccgctga agtgattgaa cagctggtct
     6721 ttggtcaggt cgtacaaatg cctgaagccc ccaacattgc gcgtgaaatt gttctcggta
     6781 ccggaatgaa tgtgcatacc gatgcttaca gcgtcagccg cgcttgcgct accagtttcc
     6841 aggcggttgc aaacgtcgca gaaagcctga tggcgggaac tatccgagcg gggattgccg
     6901 gtggggcaga ttcctcttcc gtattgccaa ttggcgtcag taaaaaactg gcgcacgtgc
     6961 tggttgatgt caacaaagct cgaaccatga gccagcgcct gaaactcttc tctcgcctgc
     7021 gtttgcgcga cttaatgccc gtaccgcctg cggtagcaga atattctact ggcttgcgga
     7081 tgggcgacac cgccgaacaa atggcgaaaa cctacggcat cacccgagaa cagcaagatg
     7141 cattagcgca ccgttcgcat cagcgtgccg ctcaggcatg gtcagacgga aaactcaaag
     7201 aagaggtgat gactgccttt atccctcctt ataaacaacc gcttgtcgaa gacaacaata
     7261 ttcgcggtaa ttcctcgctt gccgattacg caaagctgcg cccggcgttt gatcgcaaac
     7321 acggaacggt aacggcggca aacagtacgc cgctgaccga tggcgcggca gcggtgatcc
     7381 tgatgactga atcccgggcg aaagaattag ggctggtgcc gctggggtat ctgcgcagct
     7441 acgcatttac tgcgattgat gtctggcagg acatgttgct cggtccagcc tggtcaacac
     7501 cgctggcgct ggaacgtgcc ggtttgacga tgagcgaact gacattgatc gatatgcacg
     7561 aagcctttgc agctcagaca ctggcgaata ttcagttgct gggtagtgaa cgttttgctc
     7621 gtgaagtact ggggcgtgca catgccactg gcgaagtgga cgatagcaaa tttaacgtgc
     7681 ttggcggttc gattgcttac ggacatccct tcgcggcgac cggcgcgcgg atgattaccc
     7741 agacattgca tgaacttcgc cgtcgcggcg gtggatttgg tttagttacc gcctgtgctg
     7801 ccggtgggct tggcgcggca atggttctgg aggcggaata atggaaatgg catcagcgtt
     7861 tactcttaat gttcgtctgg acaacattgc tatcatcacc atcgacgtac cggatgagaa
     7921 aatgaatacc ctgaaggcgg aatttgcctc gcaggtgcgc gccattatta agcaactccg
     7981 tgaaaacaaa gagttgcgcg gcgtggtgtt tatctccgcg aaaccggaca attttatcgc
     8041 cggagcagac atcaacatga ttggcaactg caaaacggcg caagaagcgg aagcactggc
     8101 gcggcagggc caacagttga tggcggagat tcatgctttg cccattccgg ttatcgcggc
     8161 tattcatggc gcttgcctgg gcggtgggct ggagctggcg ctggcgtgcc acggtcgcgt
     8221 ttgtactgac gatcctaaaa cggtgctcgg tttgcctgaa gtacaacttg gattgttacc
     8281 cggttcaggc ggcacccagc gtttaccgcg tctgataggc gtcagtacag cattagagat
     8341 gattctcacc ggaaaacaac ttcgggcgaa acaggcatta aagctggggc tggtggatga
     8401 cgttgttccg cactccattt tgctggaagc cgctgttgag ctggcgaaga aggatcgccc
     8461 atcttcccgc cctctacctg tacgcgagcg tattctggcg gggccgttag gtcgtgcgct
     8521 gctgttcaaa atggtcggca agaaaacaga acacaaaact caaggcaatt atccggcgac
     8581 agaacgcatc ctggaggttg ttgaaacggg attagcgcag ggcaccagca gcggctatga
     8641 cgctgaagct cgcgcctttg gcgaactggc gatgacgccg caatcgcaag ctttgcgtag
     8701 tatctttttt gccagtacgg acgtgaagaa agatcccggt agtgatgcac cgcctgcgcc
     8761 attaaacagc gtgggaattt taggtggtgg cttgatgggc ggcggtattg cttatgtcac
     8821 tgcttgtaaa gcggggcttc cggtcagaat taaagatatc aacccgcagg gcataaatca
     8881 tgcgctgaag tacagttggg atcagctgga gggcaaagtt cgccgtcgtc atctcaaagc
     8941 cagcgaacgt gacaaacagc tggcattaat ctccggaacg acggactatc gcggctttgc
     9001 ccatcgcgat ctgattattg aagcggtgtt tgaaaatctc gaattaaaac aacagatggt
     9061 ggcggaagtg gagcaaaatt gcgccgctca taccattttt gcttcgaaca cgtcatcttt
     9121 accgattggt gatatcgccg ctcacgccgc gcgacctgag caagttattg gcctgcattt
     9181 cttcagtccg gtggaaaaaa tgccgctggt agagattatt cctcatgcgg gtacatcggc
     9241 gcaaaccatc gccactacgg taaaactggc gaaaaaacag ggcaaaacgc ccattgtcgt
     9301 gcgtgacaaa gccgggtttt acgtcaatcg catcttagcg ccttacatta atgaagctat
     9361 ccgcatgctg accgaaggtg aacgggtaga gcacattgat gccgcgctag tgaaatttgg
     9421 ttttccggta ggcccaatcc aacttttgga tgaggtagga atcgacaccg ggactaaaat
     9481 tattcctgta ctggaagccg cttatggaga acgttttagc gcgcctgcaa atgttgtttc
     9541 ttcaattttg aacgacgatc gcaaaggcag aaaaaatggt cggggtttct atctttatgg
     9601 tcagaaaggg cgtaaaagca aaaaacaggt cgatcctgcc atttacccgc tcattggcgc
     9661 acaagggcag gggcgactct ccgcaccgca ggttgctgaa cggtgtgtga tgttgatgct
     9721 gaatgaagca gtacgttgtg ttgatgagca ggttatccgt agcgtgcgtg acggggatat
     9781 tggtgcggta tttggcattg gttttccgcc atttctcggt ggaccgttcc gctatatcga
     9841 ttctctcggc gcgggcgaag tggttgcaat aatgcaacga ctggccacgc agtatggttc
     9901 ccgttttacc ccttgcgagc gtttggtcga gatgggcgcg cgtggggaaa gtttttggaa
     9961 aacaactgca actgacctgc aataagaagg tcaaagctat atgaatccgc gctgaatggc
    10021 ggagtgttgg tcaaaatgta aacgcatatt gactatactt acgccattga ggtaaaaaac
    10081 agcgtttcat tcggtgaatg gataaggcac aatgccggcc accgttttct ttctctggtt
    10141 tcagatgaaa gaaaacgggc gaatctggtt aacaaaagcg gtgcaatatg caagttttta
    10201 tcatgcgtca cggcgacgca gccctcgatt ccgccagtga ttcggttcgt cctctgacca
    10261 ctaatggttg tgacgaatct cgcctgatgg cgaactggct gaaaggtcaa aaagtggaaa
    10321 tcgaacgcgt tctggtgagc ccgttcctgc gagccgagca aacactggaa gaagtagggg
    10381 attgtctgaa cctgccttcc agtgcagaag ttctgccgga gttaacgccc tgcggcgatg
    10441 ttggtctggt cagtgcctat ttgcaggcgt tgaccaatga aggtgttgct tcggtactgg
    10501 tgatctctca cttaccgtta gtcgggtatc tggttgccga gttatgtccg ggcgaaacgc
    10561 cgcctatgtt taccacctcg gccattgcca gcgtaacgct tgatgaaagc ggaaacggta
    10621 cattcaactg gcagatgagc ccgtgtaatc tgaagatggc aaaagctatc tgatgtgttc
    10681 caaattatgc ccaatgaggt agattcattg ggcataattg ctcattaggc agcggatatc
    10741 tgtcaacctc actactatcg aagactctcc ttatctataa ataaaacact ttctacattc
    10801 attcggaaat tattcttctg cgcgtatctt gattttgccc aaagttacat gttgaaatga
    10861 ataatatctg tttgacactc attgtcattt attaatacag ataatagaga aaatagagtt
    10921 gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt gtttatatat
    10981 tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt gttttttaag
    11041 ctgttgatat tcattattga tatactttct attttttcta aatacttatt ccgtattttt
    11101 taaggatgtc cttaagataa cctttcttct gtcaatatat taattaaatc tacatttact
    11161 ctttagtaaa attaaattgg atcaggggtg tggaaacatc actatgcggc cccacaccaa
    11221 tgaatacaat tttctacgac ctgttctttt tgggtcgcat gccgtattca atggctgggt
    11281 ataaacatt
//
LOCUS       DAECOD010000118        11209 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.118, whole genome shotgun sequence.
ACCESSION   DAECOD010000118 DAECOD010000000
VERSION     DAECOD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.118"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1090
                     /locus_tag="KKO15_003326"
                     /pseudo
     CDS             1..1090
                     /locus_tag="KKO15_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(1214..1993)
                     /gene="glcC"
                     /locus_tag="KKO15_003327"
     CDS             complement(1214..1993)
                     /gene="glcC"
                     /locus_tag="KKO15_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBD6362005.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAI
                     CQASHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPH
                     VAQRAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2244..3743
                     /gene="glcD"
                     /locus_tag="KKO15_003328"
                     /pseudo
     CDS             2244..3743
                     /gene="glcD"
                     /locus_tag="KKO15_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3743..4795
                     /gene="glcE"
                     /locus_tag="KKO15_003329"
     CDS             3743..4795
                     /gene="glcE"
                     /locus_tag="KKO15_003329"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBD6362006.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4806..6025
                     /gene="glcF"
                     /locus_tag="KKO15_003330"
                     /pseudo
     CDS             4806..6025
                     /gene="glcF"
                     /locus_tag="KKO15_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6030..6434
                     /locus_tag="KKO15_003331"
     CDS             6030..6434
                     /locus_tag="KKO15_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBD6362007.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6456..8627
                     /gene="glcB"
                     /locus_tag="KKO15_003332"
     CDS             6456..8627
                     /gene="glcB"
                     /locus_tag="KKO15_003332"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBD6362008.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8982..10577
                     /locus_tag="KKO15_003333"
     CDS             8982..10577
                     /locus_tag="KKO15_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBD6362009.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2641 a         2773 c         3110 g         2685 t
ORIGIN      
        1 accttaaggc atttatcgct tttccgtcat cactttatcc cgacgatcca aactggatac
       61 cccctttatt cattgaacgc aacgagcatt tgtctgcgaa aaatccgggg acggaccata
      121 tcatctggca ggcgtgggtg gcaaaaaaag cggggcagat agttgggcgt attaccgcgc
      181 aaatagatac cttgcaccgt gagcgttacg gtgaagatac cggtcatttc ggcatgattg
      241 acgccattga tgatccgcag gattttgccg cgttgtttgg cgcagcggaa gcgtggttga
      301 agtcacaagg tgcaagtaag atcagcgggc ctttcagcct gaatatcaat caggaaagcg
      361 gattactgat tgaaggtttt gacacaccac cctgtgcgat gatgccacac ggcaaaccgt
      421 ggtatgccgc gcatattgaa caactgggtt atcacaaagg cattgattta ctggcgtggt
      481 ggatgcagcg aaccgatctc actttctctc cggcgctaaa aaaactgatg gatcaggtgc
      541 gcaaaaaggt gaccattcgc tgcatcaatc gtcagcggtt tgccgaagag atgcagatcc
      601 tgcgtgagat tttcaactct ggctggcagc acaactgggg atttgtgccg tttaccgaac
      661 atgaattcgc gaccatgggc gaccaactta aatatctggt gccggacgat atgatctaca
      721 tcgctgagat tgattctgca ccctgcgcgt ttattgtggc ttgcctaaca tcaacgaggc
      781 gattgccgat ctgaacggat cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt
      841 gaaagtcagc ggtgtgcgaa ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca
      901 gttcagccgc atcggcccgg tgattgcgct gttattgatt gaagccttac gcgatccgtt
      961 tgcccgccgg aagatcgatg cgctggagat gtcatggatc ctcgaaacca atacaggcat
     1021 gaataacatg ctggaacgca ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa
     1081 gcaaatttaa taccagatcc tgtttagcgc aggggcggtc attcgggaga gggtatagat
     1141 gaacagattt aacaccagat cctgttaagt gcaggggcgg tcccctcgcc ttggggatag
     1201 ggtaaaactc tgctcacatt cccaccttgt tcatctccag cggcaccgcc gagcgaatca
     1261 aatggtggcc ttccagctcg atatcgtgga gattcttttt cacggtccgt acatgatcgc
     1321 gcgctgcgcg ctgagcgacg tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat
     1381 gctggcgatc gatctgcttt ttttgctgtg gtcgatggta gagattattt acgctggcaa
     1441 acactgaatt aaacatcaga tcggtcaatg attgcagcgt aaacaccagc acctgattgt
     1501 gagaagcctg acaaatggcg agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg
     1561 aaatctcttt gtggttctca ctggcggcga gcattttttc ataacagcgg gttatcacaa
     1621 caaaatcagc ctgcgttccc agcgttccca gcgttgccgc cagccttgcc gattcgccct
     1681 ccagtaatgc gcgaacgtcg agcagatcgt acagcgttcg cggctgcgta ctgaacagat
     1741 ggatcagcgg gctggtgtcc tgctcccgat taagccgtgc gacatgagaa tcgcgaccct
     1801 gcgccgtttc aataatcccg cgcccgcgca gcacggtcag cccttcacgc agtgcggagc
     1861 gtgagaagcc gagcttttca cacagtcgac gttccgaggg aagcggctga ccgaccttca
     1921 gtacgccgtc gataattaac cgttcgatac tctctgcaac cacttcgcaa atagggcgac
     1981 gttcatcttt cattcccgga cctcgtgcac aggtaggacc aatttttctg ttcttaactc
     2041 gcaaaacacg cacatcacgc aagtgattat gttacatcaa tttaacatta agttaaccaa
     2101 gacaaggtca cagagctgga aaaaaaatgg tctgaccggt agctaaagag atagacgaaa
     2161 acgaaaagcc cgcttaataa ctgttcacag aagcagcgcg caaaaatcag ctgccacaca
     2221 acacaacaaa gcgaagccta ctcatgagca tcttgtacga agagcgtctt gatggcgctt
     2281 tacccgatgt cgaccgcaca tcggtactga tggcactgcg tgagcatgtc cctggacttg
     2341 agatcctgca taccgatgag gagatcattc cttacgagtg tgacgggttg agcgcgtatc
     2401 gcacgcgtcc attactggtt gttctgccta agcaaatgga acaggtgaca gcgattctgg
     2461 ctgtctgcca tcgcctgcgt gtaccggtgg tgacccgtgg tgcaggcacc gggctttctg
     2521 gtggcgcgct gccgctggaa aaaggtgtgt tgttggtgat ggcgcgcttt aaagagagcc
     2581 tcgacattaa ccccgttagt cgccgcgcgc gcgtgcagcc gggcgtatgt aacctggcga
     2641 tctcctaggc cgttgcgccg cataatctct attacgcgcc ggatccttcc tcacaaatcg
     2701 cctgttcaat tggcggcaat gtggcggaaa atgccggtgg cgtccactgc ctgaaatatg
     2761 gtctgaccgt acataacctg ctgaaaattg aagtgcaaac gctggacggc gaggcgctga
     2821 cgctgggatc ggacgcgctg gattcacctg gttttgacct gctggcgctg ttcaccggat
     2881 cggaaggtat gctcggcgtg accaccgaag tgacggtaaa actgctgccg aagccgcccg
     2941 tggcgcgggt tctgttagcc agctttgact cggtagaaaa agccggactt gcggttggtg
     3001 acatcatcgc caatggcatt atccccggcg ggctggagat gatggataac ctgtcgatcc
     3061 gcgcggcgga agattttatt catgccggtt atcccgtcga cgccgaagcg attttgttat
     3121 gcgaactgga cggcgtggag tctgacgtac aggaagactg cgagcgggtt aactacatct
     3181 tgttgaaagc gggcgcgact gacgtccgtt tggcacagga cgaagcagag cgcgtacgtt
     3241 tctgggccgg tcgcaaaaat gcgttcccgg cggtaggacg tatctccccg gattactact
     3301 gcatggatgg caccatcccg cgtcgcgccc tgcctggcgt actggaaggc attgcccgtt
     3361 tatcgcagca atatgattta cgtgttgcca acgtctttca tgccggagat ggcaacatgc
     3421 acccgttaat ccttttcgat gccaacgaac ctggtgaatt tgcccgcgcg gaagagctgg
     3481 gcgggaagat cctcgaactc tgcgttgaag ttggcggcag catcagtggg gaacatggca
     3541 tcgggcgaga aaaaatcaat caaatgtgcg cccagttcaa cagcgatgaa atcacgacct
     3601 tccatgcggt caaggcggcg tttgaccccg atggtttgct gaaccctggg aaaaacattc
     3661 ccacgctaca ccgctgtgct gaatttggtg ccatgcatgt gcatcacggt catttacctt
     3721 ttcctgaact ggagcgcttt tgatgctacg cgagtgtgat tacagtcagg cgctgctgga
     3781 gcaggtgaat caggcaatta gcgataaaac gccgctggtg attcagggca gcaatagcaa
     3841 agccttttta ggtcgcccag tcaccgggca aacgctggat gtgcgttgtc atcgcggcat
     3901 tgttaattac gacccgaccg agctggtgat aaccgctcgt gccggaacgc cgctggtggc
     3961 gattgaagcg gcgctggaga gcgccgggca aatgcttccc tgcgagccgc cgcattatgg
     4021 tgaagaagcc acctggggcg ggatggtcgc ctgcgggctg gcggggccgc gtcgcccgtg
     4081 gagcggttcg gtccgcgatt ttgtcctcgg cacgcgcatc attaccggca ctggaaaaca
     4141 tctgcgtttt ggtggcgaag tgatgaaaaa cgttgccgga tacgatctct cacggttaat
     4201 ggccggaagc tatggttgtc ttggcgtgct cactgaaatc tcaatgaaag tgttaccgcg
     4261 accgcgtgcc tctctgagcc tgcgtcggga aatcagcctg caagaagcca tgagtgaaat
     4321 cgccgagtgg caactccagc cattacccat tagtggctta tgttacttcg acaatgcgtt
     4381 gtggatccgc cttgagggcg gcgaaggatc ggtaaaagca gcgcgtgaac tgctgggtgg
     4441 cgaagaggtt gcgggtcagt tctggcagca attgcgtgaa caacaactgc cgttcttctc
     4501 gttaccaggt accttatggc gcatttcatt acccagcgat gcgccgatga tggatttacc
     4561 cggcgagcaa ctgatcgact ggggcggggc gttacgctgg ctgaaatcga cagccgatga
     4621 caatcaaatc catcgcatcg cccgcaacgc tggcggtcat gcgacccgct ttagtgccgg
     4681 agatggtggc tttgccccgc tatcggctcc tttattccgc tatcaccagc agcttaaaca
     4741 gcagctcgac ccttgcggcg tgtttaaccc cggtcgcatg tacgcggaac tttgaggagc
     4801 aggctatgca aacccgatta accgaagaga tgcggcagaa cgcgcgctgg aagccgacag
     4861 catcctgtgc gcctgtgttc actgcggatt ttgtaccgca acctgcccaa cctatcagct
     4921 tctgggcgat gaactggacg ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga
     4981 aggcaacgaa gtcacgctta aaacacagga gcatctcgat cgctgcctca cttgccgcaa
     5041 ttgtgaaacc acctgtcctt ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga
     5101 tattgtcgag cagaaagtga aacgcccact gccggagcga atgctgcgcg aaggattgcg
     5161 ccaggtagtg ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc tggtgctgcg
     5221 accgttttta ccggaacagg tcagagcaaa actgcctgct gaaacggtga aagctaaacc
     5281 gcgtccgccg ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg cccagcctac
     5341 gctttcgccc aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt
     5401 catgtcagct aacgaagcag gctgttgtgg cgcggtggac tatcatctta atgcgcagga
     5461 gaaagggttg gcacgggcgc gcaataatat tgatgcctgg tggcccgcga ttgaagcagg
     5521 tgccgaggca attttgcaaa ccgccagcgg ttgcggcgcg ttcgtcaaag agtatgggca
     5581 gatgctgaaa aacgatgcgt tatatgccga taaagcgcgt caggtcagtg aactggcggt
     5641 cgatttagtc gaacttctgc gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa
     5701 aaaactggcc ttccactgcc cgtgtaccct acaacatgcg caaaagctga acggcgaagt
     5761 ggaaaaagtg ttacttcgtc ttggatttac cttaacggac gttcccgaca gccatctgtg
     5821 ctgcggttca gcgggaacat atgcgttaac gcatcccgat ctggcacgcc agctgcggga
     5881 taacaaaatg aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg ccaacattgg
     5941 ttgccagacg catctggcga gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt
     6001 agaacaagcc cttgaaaagg aataaaaaaa tgaaaactaa agtcattctt agccagcaaa
     6061 tggcgagtgc aattattgcc gcaggtcagg aagaggcgca gaaaaataac tggtctgttt
     6121 ccattgctgt tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg
     6181 cgccgattgc ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg
     6241 aaactaaggg ctatgaagag atggtgaaca acggacgtac cgcgttcgtg actgcgccgt
     6301 tactgacgtc gctggaaggc ggcgtaccgg ttgttgttga tgggcaaatt attggtgccg
     6361 tgggcgtttc tggtttaacc ggagcacagg atgcgcaggt cgcgaaagtg gcagcagcgg
     6421 tgttggcgaa ataagcgaaa acgaggagag aaacaatgag tcaaaccata acccagggcc
     6481 gtttacgcat tgacgccaat tttaaacgtt ttgtggatgg agaagttttg ccgggcgtgg
     6541 aactggacgc tgctgcattc tggcacaatg ttgatgagat cgttcacgat ctggcaccag
     6601 aaaatcgtca gttgctggca gaacgcgatc gcattcaggc ggcgcttgat gagtggcatc
     6661 gcagcaatcc ggggccggta aaagataaag cggcctataa atctttcctt cgtgaactgg
     6721 gctacctggt gccgcaaccg gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa
     6781 tcaccagtca ggcggggcca cagctggtgg ttccggcaat gaacgcccgc tacgcgctga
     6841 acgcggcgaa cgctcgctgg ggctcactgt acgatgcgtt atacggcagc gacatcatcc
     6901 cgcaggaagg ggcgatggtc agtggctacg atccgcagcg cggtgagcag gttatcgcct
     6961 gggttcgacg tttcctcgat gaatcactac cgctggaaaa cggcagctat caggatgtgg
     7021 tggcgtttaa ggtggtcgat aaacaattac gcatccagtt gaaaaatggt aaagaaacca
     7081 cgttacgtac tccagcacag tttgtcggtt accgtggcga tgccgctgcg ccgacctgca
     7141 ttttgctgaa aaataacggc ctgcatattg aactgcaaat tgatgccaac gggcggattg
     7201 gcaaagacga tccggcgcac atcaacgatg ttatcgtcga agctgctatc agtaccattc
     7261 tcgactgcga agattcggtc gcggcggttg atgcggaaga taaaatcctg ctgtaccgca
     7321 atctgctggg cctgatgcag gggactctgc aagagaaaat ggagaaaaac ggtcggcaaa
     7381 tcgtacgtaa actgaatgac gatcgtcagt acaccgccgc cgatggctct gaaatttctc
     7441 tgcacggacg ctcgttgctg tttatccgca acgtgggtca tttgatgacc attcctgtga
     7501 tttgggacag cgaaggcaat gaaatcccgg aaggtattct tgatggcgtc atgactggcg
     7561 cgattgccct ctatgattta aaagtgcaga aaaactcgcg cactggcagc gtctatattg
     7621 tgaaaccgaa aatgcacggt ccgcaggaag tggcgttcgc caacaaactg tttacccgca
     7681 ttgagacaat gctcggtatg gcaccgaata ccctgaaaat gggcattatg gatgaagaac
     7741 gccggacctc gctgaacttg cgtagctgta tcgctcaggc gcgcaaccgc gtggcgttca
     7801 tcaataccgg tttcctcgac cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc
     7861 cgatgctgcg taaaaaccag atgaaatcga cgccgtggat caaagcctac gaacgtaata
     7921 acgtgctttc cggtctgttc tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt
     7981 gggcaatgcc ggacctgatg gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg
     8041 gggcaaacac agcctgggtt ccgtcaccaa ccgctgctac gctccatgcg ctgcactacc
     8101 accataccaa cgtacagagc gtgcaagcca acattgccca gaccgagttc agtgctgaat
     8161 ttgaaccgct gctggacgat ctgctgacta ttccggtggc tgaaaacgct aactggtcgg
     8221 cgcaagagat ccaacaagag ctggataaca acgtgcaggg gattctgggg tacgtggtgc
     8281 gttgggtgga gcaggggatt ggttgttcaa aagtgccgga tattcacaac gtggcgctga
     8341 tggaagaccg cgcaacgctg cgtatctcca gccagcatat cgccaactgg ttacgtcacg
     8401 gtattctgac caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc
     8461 agcaaaatgc tggcgatccg gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg
     8521 cttttaaagc tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac ggttataccg
     8581 aaccgttatt acacgcctgg cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa
     8641 gccccggcaa gttttattgt cggggctatg atagtaactg cggtagtaca tttatcccgg
     8701 aaataaccgg agcgattatc agggtttaac aatattaaat acccgtagcg cattactagc
     8761 gggcctaaaa caacaatatg gacatcttaa cgatactctg cgcctgcgtg tgcggaacag
     8821 aaacccttac ccgatatact tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt
     8881 tgctgtaaag tataacgcct atattttaca gttcagtcct cttttgctta ttacaacctt
     8941 aagtatatca agcatataaa gataataaga gactgaacaa tatggttacc tggacccaaa
     9001 tgtatatgcc gatgggagga ctggggctat ccgctctggt cgccctgatc ccgataatat
     9061 tcttcttcgt tgcactcgcg gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc
     9121 ttatattatc tatcctgatt gcgatattcg cctttaaaat gccgattgat atggcatttg
     9181 ctgctgcggg ctatggcttt atttatggat tatggccaat tgcgtggatt attgtcgcgg
     9241 cggtgttcct gtataaatta accgttgcca gcgggcagtt cgatattatc cgcagctcgg
     9301 ttatctccat caccgacgat cagcgtttgc aggtgttact gattggtttc tcctttggtg
     9361 cgttgctgga aggagctgct ggctttggtg cgacggtggc gtttggtgcg ttgggcgtgc
     9421 cgattctggt cgccggtcag gtaacgggaa tcgatccgtt ccacattggc gcaatggcgg
     9481 gacgtcagtt accgttcctg tcggttcttg tgccgttctg gctggtagca atgatggacg
     9541 gctggaaagg ggtgaaagag acgtggccag cggcgctggt tgctggggga agcttcgctg
     9601 tcactcagtt ctttacctct aactatattg gtccggaact gccggatatt acttcggcgc
     9661 tggtgagtat cgtctcactc gctttatttc ttaaagtctg gcggccgaaa aataccgaaa
     9721 cggcaatcag catggggcaa tccgcaggtg cgatggtggt aaataagcca tcttctggcg
     9781 gtcccgtgcc ttcagaatat agtctggggc aaatcattcg tgcgtggtca ccgtttttaa
     9841 tcttaacggt gctggtcacc atctggacca tgaagccgtt taaagcgtta tttgctccgg
     9901 gcggcgcgtt ttattcactg gtgattaatt tccagatccc tcatttgcat caacaagtgt
     9961 tgaaagcggc acccattgtc gcccaaccaa cgccaatgga tgcggtgttt aaattcgacc
    10021 ctctctcggc tggcggcacc gctattttta ttgcggcgat tatctctatc ttcatcctcg
    10081 gcgtgggcat caagaaaggg attggcgtct ttgccgaaat gctaattagt ctgaagtggc
    10141 cgatattgtc gattggcatg gtgctggcat tcgccttcgt caccaactat tccggcatgt
    10201 ccaccacgct ggcgctggta ctggcaggta caggcgtgat gttcccgttt ttctcaccgt
    10261 ttctcggctg gctgggcgta ttccttaccg ggtcggacac ttcttcaaac gccctgtttg
    10321 gttcactgca atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg ctggtggcag
    10381 caaataccag cggcggcgta actggcaaga tgatctcccc gcaatctatc gccgtggcct
    10441 gcgccgcgac gggcatggtg ggccgagaat ctgaactgtt ccgctacacc gtgaagcaca
    10501 gtctgatttt tgccagcgtt atcggcgtta tcaccctgct gcaggcgtat gtgtttaccg
    10561 ggatgttagt ctcgtaaatc aataaagccg gatgaaggta aatatcaccc ggctttccat
    10621 ttgtctgtcg tttatctcgc cagggaaccg tttttacgat tggcttcttt atacgtagtc
    10681 tccgttttac gatgcgaatt gtttaatatt tatacaactt ggcttctgtg ctggttataa
    10741 agtacccact ttcatgagat caattttagt atgttattac attgcttcat ctgttgcttt
    10801 catgacgaat ggcgtttttt ctcttattta tatttttcac tagaacaatg agtagatcaa
    10861 aattcccaaa actcccaaaa acataagatt attcgcaaag ctgattttgc agaataatga
    10921 gaacttttaa gatttgtcga ggatcaattt taagcaattt atctccactt gttacataaa
    10981 agatgccgtt aggttaataa gttaatcttg ctataacagt gttcgttact gcctattaat
    11041 aaagatattt gggatagggt aacgtggatg aggattcgaa atcatccgtc ttgcggaaag
    11101 tgaaatgttg gcagatgtta atattatgta tgtattttaa ctccaacaac acaccaatca
    11161 ttttgttaga aataagaaaa tttatggtta aataatttgg tgattatga
//
LOCUS       DAECOD010000119        11036 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.119, whole genome shotgun sequence.
ACCESSION   DAECOD010000119 DAECOD010000000
VERSION     DAECOD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.119"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            155..505
                     /locus_tag="KKO15_003334"
     CDS             155..505
                     /locus_tag="KKO15_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBD6362010.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            558..953
                     /locus_tag="KKO15_003335"
     CDS             558..953
                     /locus_tag="KKO15_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBD6362011.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="KKO15_003336"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="KKO15_003336"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBD6362012.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="KKO15_003337"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="KKO15_003337"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBD6362013.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="KKO15_003338"
     CDS             complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="KKO15_003338"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBD6362014.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLSAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="KKO15_003339"
     CDS             complement(5324..6424)
                     /locus_tag="KKO15_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBD6362015.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="KKO15_003340"
     CDS             complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="KKO15_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBD6362016.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7572..8138)
                     /locus_tag="KKO15_003341"
     CDS             complement(7572..8138)
                     /locus_tag="KKO15_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6362017.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8354..10087
                     /gene="argS"
                     /locus_tag="KKO15_003342"
     CDS             8354..10087
                     /gene="argS"
                     /locus_tag="KKO15_003342"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBD6362018.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10264..10752
                     /locus_tag="KKO15_003343"
     CDS             10264..10752
                     /locus_tag="KKO15_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBD6362019.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2692 a         2816 c         2632 g         2896 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcgct cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gtatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
     6481 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
     6541 atttattaca atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat
     6601 atttatcagt tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag
     6661 cacaacgcta tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata
     6721 ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
     6781 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
     6841 ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt
     6901 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
     6961 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
     7021 ggactcctgc tccagccata atgattggag catcacgatg ggcaataagt tccataattt
     7081 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
     7141 attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa
     7201 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat
     7261 tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc
     7321 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
     7381 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca
     7441 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
     7501 tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt
     7561 ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga
     7621 aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc
     7681 tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     7741 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata
     7801 tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa
     7861 aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     7921 ttgatcattt tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca
     7981 gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc
     8041 aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     8101 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     8161 aaagcgttaa tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg
     8221 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     8281 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     8341 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     8401 tgcggcaggc gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt
     8461 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     8521 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     8581 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     8641 tcagcaggcg ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt
     8701 ggttgactac tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac
     8761 cattattggt gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     8821 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     8881 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     8941 gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa
     9001 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     9061 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat
     9121 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     9181 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga
     9241 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     9301 tgatatcgcc tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta
     9361 catcgactcc cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg
     9421 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     9481 cggcaaaccg ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga
     9541 agccctggag cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga
     9601 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     9661 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     9721 cgcgccatac atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat
     9781 taacgaagag caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact
     9841 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     9901 gcatgtaatg tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca
     9961 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
    10021 actgacagcg aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg
    10081 tatgtaatcg atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt
    10141 attgccatct aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag
    10201 aggccagaag cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc
    10261 gaagtgttta aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa
    10321 gatacggtta tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat
    10381 tcagatgatg atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc
    10441 gtcgatgcgc aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca
    10501 cgcacaggtg caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga
    10561 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag
    10621 gatttaagtg acccctattc tgaatcagaa tggctatcat gtatgatcat acagaccaat
    10681 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
    10741 acaagaggat aattcacatc tttttggcat gttttgttgc aagctattcc tgataaatga
    10801 ttgcaacaaa acatccagcc ttttgactga gttattaaac atactcgcga gcgcgtaatt
    10861 ttttgtcttt cagcgataat tcacaatcac ttcattccgt tgtaccttta gcgtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAECOD010000120        10983 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.120, whole genome shotgun sequence.
ACCESSION   DAECOD010000120 DAECOD010000000
VERSION     DAECOD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.120"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..177)
                     /locus_tag="KKO15_003344"
     CDS             complement(<1..177)
                     /locus_tag="KKO15_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD6362020.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="KKO15_003345"
     CDS             complement(380..847)
                     /locus_tag="KKO15_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD6362021.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="KKO15_003346"
     CDS             complement(844..1377)
                     /locus_tag="KKO15_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD6362022.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="KKO15_003347"
     CDS             complement(1443..2072)
                     /locus_tag="KKO15_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362023.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="KKO15_003348"
     CDS             complement(2076..2291)
                     /locus_tag="KKO15_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6362024.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2584..2658)
                     /locus_tag="KKO15_003349"
     tRNA            complement(2584..2658)
                     /locus_tag="KKO15_003349"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Gly,seq:tcc)
     gene            complement(2662..2737)
                     /locus_tag="KKO15_003350"
     tRNA            complement(2662..2737)
                     /locus_tag="KKO15_003350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2702..2704),aa:Thr,seq:tgt)
     gene            complement(2740..2816)
                     /locus_tag="KKO15_003351"
     tRNA            complement(2740..2816)
                     /locus_tag="KKO15_003351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2780..2782),aa:Arg,seq:tct)
     gene            complement(2818..2894)
                     /locus_tag="KKO15_003352"
     tRNA            complement(2818..2894)
                     /locus_tag="KKO15_003352"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Ile,seq:cat)
     gene            complement(3094..3474)
                     /locus_tag="KKO15_003353"
     CDS             complement(3094..3474)
                     /locus_tag="KKO15_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD6362025.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3467..3667)
                     /locus_tag="KKO15_003354"
     CDS             complement(3467..3667)
                     /locus_tag="KKO15_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBD6362026.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3762..4019)
                     /locus_tag="KKO15_003355"
     CDS             complement(3762..4019)
                     /locus_tag="KKO15_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362027.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4016..5407)
                     /locus_tag="KKO15_003356"
     CDS             complement(4016..5407)
                     /locus_tag="KKO15_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD6362028.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5404..6282)
                     /locus_tag="KKO15_003357"
     CDS             complement(5404..6282)
                     /locus_tag="KKO15_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD6362029.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6293..7201)
                     /locus_tag="KKO15_003358"
     CDS             complement(6293..7201)
                     /locus_tag="KKO15_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6362030.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7188..7421)
                     /locus_tag="KKO15_003359"
     CDS             complement(7188..7421)
                     /locus_tag="KKO15_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362031.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7418..8434)
                     /locus_tag="KKO15_003360"
                     /pseudo
     CDS             complement(7418..8434)
                     /locus_tag="KKO15_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8366..8629
                     /locus_tag="KKO15_003361"
     CDS             8366..8629
                     /locus_tag="KKO15_003361"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD6362032.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8619..8927
                     /locus_tag="KKO15_003362"
     CDS             8619..8927
                     /locus_tag="KKO15_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6362033.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8969..9145)
                     /locus_tag="KKO15_003363"
     CDS             complement(8969..9145)
                     /locus_tag="KKO15_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBD6362034.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(9142..9552)
                     /locus_tag="KKO15_003364"
     CDS             complement(9142..9552)
                     /locus_tag="KKO15_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBD6362035.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9549..9827)
                     /locus_tag="KKO15_003365"
     CDS             complement(9549..9827)
                     /locus_tag="KKO15_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362036.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9902..10726)
                     /locus_tag="KKO15_003366"
     CDS             complement(9902..10726)
                     /locus_tag="KKO15_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362037.1"
                     /translation="MVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLEL
                     KPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARR
                     RYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKP
                     TGKMDVKGSGAITGLTDKLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLML
                     EKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2631 a         2955 c         2512 g         2885 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg cactgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     2521 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     2581 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     2641 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     2701 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     2761 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     2821 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac
     2881 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     2941 gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg
     3001 cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac
     3061 gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat
     3121 ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag
     3181 cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat
     3241 cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg
     3301 ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac
     3361 ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg
     3421 attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt
     3481 ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt
     3541 tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg
     3601 aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga
     3661 cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata
     3721 atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc
     3781 accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     3841 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     3901 tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc
     3961 atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa
     4021 agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg
     4081 gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt
     4141 tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt
     4201 ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa
     4261 tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt
     4321 tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac
     4381 cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc
     4441 ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat
     4501 atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt
     4561 ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt
     4621 cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag
     4681 acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat
     4741 gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag
     4801 gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt
     4861 catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac
     4921 gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg
     4981 tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg
     5041 tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg
     5101 cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac
     5161 gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt
     5221 caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc
     5281 gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc
     5341 cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag
     5401 ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca
     5461 gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc
     5521 ttccggtgag aggttactga tcagaatggt gggcaagata ctttcgtacc tggcgttgat
     5581 aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat
     5641 gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc
     5701 attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact
     5761 ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt
     5821 accacaactg ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc
     5881 cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt
     5941 atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc
     6001 cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca
     6061 gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg
     6121 atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg
     6181 ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc
     6241 ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg
     6301 tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat
     6361 cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag tcttcgaagt
     6421 gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg gtatttcctg
     6481 tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca ggttttattc
     6541 cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta ccaccagcac
     6601 gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt cgggaagaac
     6661 gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct
     6721 ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc
     6781 gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag
     6841 tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat
     6901 taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa
     6961 ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc
     7021 cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat
     7081 cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa
     7141 cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca gacggacgca
     7201 tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag cggataactc
     7261 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt actgaccagg
     7321 caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     7381 ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc tccttgtcag
     7441 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     7501 acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc tggcttgtcc
     7561 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt gcgacagatg
     7621 acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag gcattgatgt
     7681 atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg aaggcgaagc
     7741 cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc tggcgtacca
     7801 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     7861 tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc agggatgaag
     7921 taacagccta accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     7981 tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca atcacagagg
     8041 tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca tcttgcgatg
     8101 tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa aaaaccgcat
     8161 gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc gctttataag
     8221 gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca gatataacga
     8281 ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag aggtccgact
     8341 ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg ttgaacgcat
     8401 gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg acgggctttt
     8461 cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca ctctgacagg
     8521 gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac cgccagccga
     8581 acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa cgcactctcc
     8641 ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct tttaaagcag
     8701 aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca gcacgcaaag
     8761 aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg accgcgatca
     8821 ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc acactaaaaa
     8881 aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca caccgcgccg
     8941 tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt agccaccgga
     9001 tatcccacag gtgagccgtg tagttgaagg tttttacgtc agattctttt gggattggct
     9061 tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg
     9121 tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga caccctgacc
     9181 aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc tcatctgcgc
     9241 gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat cagcttcctg
     9301 ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg gttttaacca
     9361 tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa attttgcgtt
     9421 ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt ctttatcgag
     9481 aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc ggaggaagat
     9541 tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc tttcattact
     9601 gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc ttcttcacca
     9661 tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat agctgctcgc
     9721 gatgcctgcc atatcaccca agcaaactct tttaattcat cgtctcctgt gaactggctt
     9781 ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc cctctccccc
     9841 aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg tagatggtca
     9901 tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat ttcggcatat
     9961 tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc gattgctcgt
    10021 caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt tcaagcatca
    10081 aaacagatgc cggagatgcc agtagctgtt cgtccttctc tgacatcttt tcaccactct
    10141 gaactctctg taacgctctc tcgcgagcct tgttacgcca gatgatgaaa agtttgtctg
    10201 tcaggcctgt tatcgctcca gagcctttta cgtccatttt cccggttggt ttttcttcgc
    10261 tgtctccttt tcgcgagtga gtaacgagaa tgacgtggga gtttgttttg tttttgaagt
    10321 cgcaaatcga gtcaacaaac gccttctgcc cgttatagtc atcgtcgcct atgccacatt
    10381 tcatcaggct gtcgatgatg aataactgga tcccgtatcg gcggcgagcg tagtcgaata
    10441 tttcgatcag cctgtcggct ttcgccgttc cagtcaggcc aaacacccaa agtctttcgt
    10501 cataaaattt aaatgcagag tcaatttcca gcactggcgg catcttgcag cacgttgcct
    10561 gacgggtaag tcgcttaagg agaataccag gcttcagctc aagtgacgcg atgcacgtct
    10621 tcacaccctg acgcattgcc tcaagtgcca tatgcccgac aacctcggtg ttatgcgtcg
    10681 ccacatatcc cctggttaca tacaggtggc gagggtgatc gaccataata cacaagcatt
    10741 ctgcattgcc gattttttca acattcctga cgaatacccc acagccctcg aatcgatgcg
    10801 cagttaatct gccattgagt cgtggtgaac ggatggcctc cctgatttct cttgtcagtc
    10861 tgattcttgc ctcgtatgaa tccatcccat gccgcttatc gtccttgtat gtgtatgtca
    10921 cgccagtttt aactcgcgtc cggcatgacc cgccgagtga gttaaccagt tgaacaaccc
    10981 cat
//
LOCUS       DAECOD010000121        10698 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.121, whole genome shotgun sequence.
ACCESSION   DAECOD010000121 DAECOD010000000
VERSION     DAECOD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.121"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..192)
                     /locus_tag="KKO15_003367"
     CDS             complement(<1..192)
                     /locus_tag="KKO15_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD6362038.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDL"
     gene            683..814
                     /locus_tag="KKO15_003368"
     CDS             683..814
                     /locus_tag="KKO15_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBD6362039.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1074..2069
                     /gene="pgl"
                     /locus_tag="KKO15_003369"
     CDS             1074..2069
                     /gene="pgl"
                     /locus_tag="KKO15_003369"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD6362040.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSMLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2110..3063)
                     /locus_tag="KKO15_003370"
                     /pseudo
     CDS             complement(2110..3063)
                     /locus_tag="KKO15_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3247..4299
                     /locus_tag="KKO15_003371"
     CDS             3247..4299
                     /locus_tag="KKO15_003371"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBD6362041.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4375..5808
                     /locus_tag="KKO15_003372"
     CDS             4375..5808
                     /locus_tag="KKO15_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBD6362042.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5991..8171
                     /locus_tag="KKO15_003373"
     CDS             5991..8171
                     /locus_tag="KKO15_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBD6362043.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8312..9595)
                     /locus_tag="KKO15_003374"
     CDS             complement(8312..9595)
                     /locus_tag="KKO15_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBD6362044.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9730..10611)
                     /locus_tag="KKO15_003375"
     CDS             complement(9730..10611)
                     /locus_tag="KKO15_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6362045.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2628 a         2462 c         2664 g         2944 t
ORIGIN      
        1 caggtccagt ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt
       61 acgctgttct tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt
      121 gggggatgta attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt
      181 aatttctcgc atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc
      241 ctaatgagga gggtgtttca gaatatttgc cataagggaa tgagtgttat atcatgcttt
      301 ctaccctggt aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt
      361 acatattcac aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca
      421 acatctccaa ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa
      481 tcatgtataa aataagctta tatacatagt aacttcagca aaggatttag taatgatcaa
      541 aatcatatct caatgtactg tttttttctt taaaaaaaca gtacattcta ttttataatg
      601 actgtgtatt cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt
      661 cttgttaaca gggggattga ccatgcttat tggctatgta cgcgtatcaa caaatgacca
      721 gaataccgat ctacaacgta atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg
      781 aatgggaagt tggccagaag ttgttatggc atagtgttta cggtcgttga tacaggtaaa
      841 caaaagcggg gagagtcata ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa
      901 ttcgtgtcgg tgactaatat tttagtaata tgccattcga aatggagtgc taacttattg
      961 atttaattga tgtgcgatat tgtattttat cgtgttttgt tgtctgtttt ttatccagta
     1021 agtgttgtta tatgtatgaa agataaggga tttatcattt ttgaggtgtg ttcatgaagc
     1081 aaacagttta tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg
     1141 aaggcgcact gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg
     1201 tcagcccgga caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt
     1261 atcgtattgc cccggacgat ggcgcactga cctttgccgc agagtctgtg ctgccgggta
     1321 gtccgacgca tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg
     1381 cgggtaacgt gagcgtaacg cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag
     1441 tcgaggggct ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg
     1501 ttccggcatt aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg
     1561 tggcgcagga ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat
     1621 tccatccaaa cgaacaatat gcgtattgcg tcaatgagtt aaacagctca gtggatgtct
     1681 gggaactgaa agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg
     1741 aaaacttctc cgacacccgt tgggcggctg atattcatat caccccggat ggtcgccatt
     1801 tatacgcctg cgaccgtacc gccagtctga ttaccgtttt cagcgtttcg gaagatggca
     1861 gcatgttgag taaagaaggc ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg
     1921 attacagcgg caagtatctg attgccgctg ggcaaaaatc tcaccacatc tcggtatacg
     1981 aaattgttgg cgagcagggg ctactgcatg aaaaaggccg ctatgcggtc gggcagggac
     2041 caatgtgggt ggtggttaac gcacactaac cgctgattta accggcgcag tctctcctgc
     2101 gccggtgtat taacttatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca
     2161 caaattgtct ttatcgtcag tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc
     2221 cggagaaagc acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc
     2281 caccagtggg ataccaataa tcgaagaata gggcgcatgg ggcattgcca gaccaggaag
     2341 cgccgaaatc cccagtcctg cctccactag tcctaatgac gtcgaaagat gacgcacttc
     2401 gtaaaaccag tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt
     2461 gccgctggag gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag
     2521 ctactttttg gccaatggat ggtcacgccg acaggccagc acgaatggct cgttgaccag
     2581 tggggtaaaa tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc
     2641 gttacacagc accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat
     2701 attgggatac agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt
     2761 cggaatacac gctaatgtca ccataccacg atgataagca ttcatatcac gaatattaaa
     2821 aagcgtttca tcgaatttct ttattaattc ccgcgcctcc gggagcaacc tttttcctgc
     2881 ttttgttaac gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc
     2941 catctttttt attcttcgcg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt
     3001 gttaaatgaa ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt
     3061 cattattact ccggaaaatg gaagcgacga ttttgggggg ctggtcgtta aaaattttaa
     3121 ctgcatttag ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt
     3181 agagatgctt tgtccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc
     3241 attattatga aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc
     3301 ctgttagcgg aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt
     3361 atgggttccg gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt
     3421 aaagtcgcca ttattagccg ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct
     3481 caagttatcg tccatgagca acgtgtcgat accacgccta actgcggcaa tatgctgtct
     3541 ggcgtagggg cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgt
     3601 gtacgtatcc gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat
     3661 ggtgttgtcg agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg
     3721 gttgcgctca ctttcctgaa tgccgctgga acgaaaaccg gaaaagtttt cccgactgat
     3781 aatcagattg attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc
     3841 gtcattattc cggctgaata tctgggaaaa acaggttatg aattaccggc ggaactggat
     3901 gccgacaaag cattattagc ccgcattgaa tctatccggc tacaagcggg taaagcaatg
     3961 ggcttaggtg atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag
     4021 aaaggcgggg caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg
     4081 ataaccggtg ctattgctat ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa
     4141 atcgtccctt ctgtaggata tggcaatatc aatattgaac accccagtgg tgcgctcgac
     4201 gttcatttaa gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca
     4261 accagaaaaa tattttccgg tgaagtttat cttccctgaa aaaattcgtt gtcaggataa
     4321 ggacaatcaa taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat
     4381 aagaaatcgt tatggaagct aattctgata ttagcgatcc catgtattat tggttttatg
     4441 ccagctccgg caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc
     4501 attgtgggtc tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct
     4561 gcctcaatgg tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta
     4621 agcggttact cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca
     4681 tttgtgacca ccggtttagg taaacttatt gcctatctgc tgattggtaa aatcggtaac
     4741 accatgctgg gtctgggtta cgttacggta ttcctcgatc tggtactggc tccggcaaca
     4801 ccgtctaaca ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcggtg
     4861 gctttggggt ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc
     4921 atttacatgg tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt
     4981 ctggcgctga aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg
     5041 ctggccgccg gattgccggg catcattatg ctgctggtca ccccgctggt aatttacacc
     5101 atgtatccac cagaaattaa aaaggtggat aacaaaacca tcgctaaagc gggccttgcc
     5161 gaactgggac cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta
     5221 ctgggctgga ttttcagtaa gtctctgggg gttgatgaat ccactgtggc aatcgttgtt
     5281 atggcgacca tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc
     5341 ggctggaata ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa
     5401 gttaaatttt tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac
     5461 ggtaacgttg ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct
     5521 tccggtagtg cctatatcgt tgctatgtta cctgtatttg ccatgctggc gaacgtctcc
     5581 ggcgcaccgt taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg
     5641 gttactcact atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta caacgatatt
     5701 aaatcctggt ggttggtcgg tgcggtactg acgatattaa ccttcttggt gcatatcacc
     5761 ctcggcgtgt ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca
     5821 tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag
     5881 ctcctgggga ttcactcact tgtcgccttc ctgcaactcg aattatttag agtatatcca
     5941 tttattatct ttctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt
     6001 tatctgaaaa aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca
     6061 ccggcgaaat taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt
     6121 ctcataatac gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc
     6181 acgatattac ctttgttggt attgtacaga ccgctaaagc gtccggaatg gaacgtttcc
     6241 cgctgccgta tgtgctgacc aactgccata actcactctg cgccgtcggc ggcaccatta
     6301 acggtgatga ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc
     6361 ctccgcatat tgcggtcatc catcaatata tgcgtgagat gatggctggc gggggcaaaa
     6421 tgatcctcgg gtcagacagc cacacccgtt acggtgcatt agggacaatg gcagtcggtg
     6481 agggtggcgg tgagttggta aaacagctgc ttaatgacac ctgggatatc gactatccgg
     6541 gcgtggttgc ggtgcatctg accggaaaac ctgcgccgta tgtggggcca caggatgtgg
     6601 cgctggctat cattggcgcg gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt
     6661 tcgttggacc gggcgttagc gcgctctcta ccgatttccg taacagcgtt gacgtgatga
     6721 ccacggaaac cacctgttta agctccgtct ggcaaactga tgaagaagtg cataactggc
     6781 tggcgctgca cggtcgcggc caggattact gccagcttaa ccctcaaccg atggcgtact
     6841 acgatggctg catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc
     6901 acccgagcaa cgtgtatgaa atcgacacac tgaaccagaa cctgaccgac attctgcgtg
     6961 agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggata
     7021 aagtggaaaa tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta
     7081 actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca
     7141 ccttctcgct ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag
     7201 gtgtggtagc agatttgatt ggcgcaggcg caatcatcag aaccgcattc tgtggcccat
     7261 gctttggcgc gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta
     7321 acttcccgaa ccgcgaaggc tctaagccag ccaatgggca gatgtcagcg gtggcgttga
     7381 tggacgctcg ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg
     7441 aacttgattg ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaacc
     7501 gtgtttatca gggctttgtg aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca
     7561 ttaaagactg gccggaattg ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga
     7621 agatcctcga cgaagtcacc accaccgacg aactgattcc ttccggtgaa acctcttctt
     7681 atcgttcaaa tccgattggt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc
     7741 gcattaagca gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca
     7801 tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc
     7861 gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc taccgttcta
     7921 acgtcatcaa ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg
     7981 gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta
     8041 aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctttac atggaaagcc
     8101 tgacggctga agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc
     8161 gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg
     8221 atgctggcgc atcttatccg acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt
     8281 tacgccgcat ccggcaaata gttaatagct cttacttctt cgcctctgca accactttgc
     8341 tgcccacgcc gcggttattg tattcccaca tgcggttgta gttagtgtca ttcagattgc
     8401 gctgtatttc gtcgttatca tctacgctgc cggtattacc cgcaaacgga cgattagaga
     8461 tcaccgcatc ggcccacggt ttagccgtgt taaaaccttc gttgatggcg ctatcacgga
     8521 tcaccacctg accgttggta ttagcatcaa catccagcga gcggcccagt tgcgccacgc
     8581 catcaccgga agcattgaaa cggctgttta cggcgaggaa accgtaatag atgttagaca
     8641 gcgtagccgg tgcaaacaca tatgcttctt gctgggtacg tgagttcacc acgcggaatt
     8701 cggtgttatc gaacaccact gcgccgcgac cagaaacgat atccacatcc ccttcaatat
     8761 agctgttggt caccagcgta cgcggctgac ggttggtttc cagacggttc tgcacaccgc
     8821 tgttggtgac aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta atctgtactt
     8881 ggtcaccgtc agtacgcagt gctaccgccg gatggttacc tgcatctacg ctatcgccca
     8941 gcgtgttttc gatggtcaga ttttgcagtt gcagtccatt gttttgtgac cagaagaccg
     9001 cagaacacag aacaccgata ctgtcgctgc gtttgctctg gcagctatcg tacatatacc
     9061 acgctggttt acctggcata tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag
     9121 ggctcatgcc cccatcaagg gaaagcccaa tcttcacatc aatcggtttt tcacctgtac
     9181 cgtacagagt gattccacct ggagcagcag ggacatacac cgttccctga tactcaccag
     9241 gcatcacggc aatatactgg cgcttgttgg tgcgcttgat aattgccgca tctaccgccg
     9301 cctgaatcgt ggtatgcgtt acaccttgag tacccgccgg gccgacaaca aagtcaggtt
     9361 gcgcaggcag ggttatcggg gacggattcc acgctgcagc acctggtgtc agggatgcaa
     9421 aatagtgttg cgcatcgaaa ttcagcgctt cttttgccga cagaatcggg cgagaagagg
     9481 taccaggcgc agtttgatca gaaggacgtt gatcgggtgg tgttgagcta caggcggtca
     9541 gcgtcacgcc aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt
     9601 gctccgggct atgaaataga aaaatgaatc cgttgaagcc tgctttttta tactaagttg
     9661 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcatt atcagtcaaa
     9721 ataaaatcat tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg
     9781 tgatgccatg gtgtccgact tgtgcccgag aagatgttga gcaaacttat cgcttatctg
     9841 cttctcatag agtcttgcag acaaactgcg caactcgtga aaggtaggtg gatccccttc
     9901 gaaggaaaga cctgatgctt ttcgtgcgcg cataaaatac cttgatactg tgccggatga
     9961 aagcggttca cgacgagtag atgcaattat ggtttctccg ccaagaatct ctttgcattt
    10021 atcaagtgtt tccttcattg atatcccgag agcatcaaca tgcaatgttg tagggatggc
    10081 aatttttacg cctgttttgc tttgctcgac ataaagatat ccatctacga tatcagacca
    10141 cttcatttcg cataaatcac caactcgttg cccggtaaca acagccagtt ccattgcaag
    10201 tctgagccaa catggtgatg attctgctgc ttgataaatt ttcaggtatt cgtcagccgt
    10261 aagtcttgat ctccttacct ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt
    10321 tatatggcct tcagctattg cctctcggaa tgcatcgctc agtgttgatc tgattaactt
    10381 ggctgatgcc gccttgccct cgtctatgta tccattgagc attgccgcaa tttcttttgt
    10441 ggtgatgtct tcaagtggag catcaggcag ccccctcctt attgctttaa ttttgctcat
    10501 gtaatttatg agtgtcttct gcttgattcc tctgctggcg aggatttttt cgtagcgatc
    10561 aagccatgaa tgtaacgtaa cagaattatc actgttgatt ctcgctgtca gaggcttgtg
    10621 tttgtgtcct gaaaataact caatgttggc ctgtatagct tcagtgattg aggtgtactg
    10681 gcaatagcgg acactacc
//
LOCUS       DAECOD010000122        10663 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.122, whole genome shotgun sequence.
ACCESSION   DAECOD010000122 DAECOD010000000
VERSION     DAECOD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.122"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            24..935
                     /locus_tag="KKO15_003376"
     CDS             24..935
                     /locus_tag="KKO15_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6362046.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            1070..2290
                     /gene="mlc"
                     /locus_tag="KKO15_003377"
                     /gene_synonym="dgsA"
     CDS             1070..2290
                     /gene="mlc"
                     /locus_tag="KKO15_003377"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBD6362047.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSCIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2415..3110
                     /gene="bioD"
                     /locus_tag="KKO15_003378"
     CDS             2415..3110
                     /gene="bioD"
                     /locus_tag="KKO15_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD6362048.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3063..4355)
                     /gene="clcB"
                     /locus_tag="KKO15_003379"
     CDS             complement(3063..4355)
                     /gene="clcB"
                     /locus_tag="KKO15_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBD6362049.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4514..5128)
                     /gene="dmsD"
                     /locus_tag="KKO15_003380"
     CDS             complement(4514..5128)
                     /gene="dmsD"
                     /locus_tag="KKO15_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBD6362050.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5171..6025)
                     /locus_tag="KKO15_003381"
     CDS             complement(5171..6025)
                     /locus_tag="KKO15_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD6362051.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(6027..6644)
                     /gene="dmsB"
                     /locus_tag="KKO15_003382"
     CDS             complement(6027..6644)
                     /gene="dmsB"
                     /locus_tag="KKO15_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD6362052.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6655..8716)
                     /locus_tag="KKO15_003383"
                     /pseudo
     CDS             complement(6655..8716)
                     /locus_tag="KKO15_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(8777..9886)
                     /locus_tag="KKO15_003384"
     CDS             complement(8777..9886)
                     /locus_tag="KKO15_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD6362053.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            9869..>10663
                     /locus_tag="KKO15_003385"
     CDS             9869..>10663
                     /locus_tag="KKO15_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD6362054.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT         2645 a         2720 c         2756 g         2542 t
ORIGIN      
        1 atctcaaata agatgtttta aatatgaata ttgaacttcg tcatctgcgt tactttgttg
       61 ctgttgcgga agagctgcat tttgggcgcg cggctgcccg cctgaatatt tcgcaaccgc
      121 cgctaagtca gcagattcag gcactggagc aacaaatcgg tgcccgactg ctggcacgaa
      181 ctaatcgcag tgtattgctg acggcagcag gaaaacagtt tcttgcagat agtcggcaaa
      241 tcctgtctat ggtggatgac gctgccgctc gcgctgaaag actgcatcag ggtgaagcgg
      301 gggagttgcg tattggtttt acttcgtccg ctccttttat tcgggcggtg tccgatacgt
      361 tatcgctgtt tcgccgtgat tatcctgatg tccatttaca aacccgcgaa atgaacactc
      421 gcgagcaaat cgctccgctc attgaaggaa cgctggatat gggattgctg cgtaacacag
      481 cgttaccgga gacgcttgaa cacgcagtca tcgtccatga accgcttatg gcgatgatcc
      541 cgtacgatca tcccctggca aataatccga gtgtaacact ggctgaactg gctgaactgg
      601 ctgaactggc gaaagaaccc tttgtctttt ttgatccgca cgtcgggact gggctgtatg
      661 acgatattct cggactgatg cgacgttacc atttgacgcc cgtcatcact caggaggtgg
      721 gcgaggcaat gaccatcatc ggtctggttt ccgccggtct gggtgtttca attttgcctg
      781 cgtcatttaa acgtgttcag ctcaacgaaa tgcgctgggt gccgattgct gaagaggatg
      841 cggtttctga aatgtggttg gtctggccga aacatcatga acaaagtcca gctgcgcgta
      901 actttcgtat tcatctgctg aatgctctca ggtgagggaa atttcagcga aaaagcccga
      961 aaaatgtgct gttaatcaca tgcctaagta aaaatttgac gacacgtatt gaagtgcttc
     1021 accatagcct acagattatt tcggagcgcg aaaatatagg gagtatgcgg tggttgctga
     1081 aaaccagcct gggcacattg atcaaataaa gcagaccaac gcgggcgcgg tttatcgcct
     1141 gattgatcag cttggtccag tctcgtgtat cgatctttcc cgtctggcgc agctggctcc
     1201 tgccagtatc actaaaattg tccgtgagat gctcgaagca cacctggtgc gagagctgga
     1261 aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg ctggtggttg aaactgaagc
     1321 ctggcactat ctttctctgc gcattagtcg cggggagatt ttccttgctc tgcgcgatct
     1381 gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg ttaaaagatg acttgccatt
     1441 gctggatcgt atcatttccc atatcgatca gttttttatc cgccaccaga aaaaacttga
     1501 gcgtctaact tcgattgcca taaccctgcc gggaattatt gatacggaaa atggtattgt
     1561 acatcgcatg ccgttctacg aggatgtaaa agagatgccg ctcggcgagg cgctggagca
     1621 gcataccggc gtaccggttt atattcagca tgatatcagc gcatggacga tggcagaggc
     1681 cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag gtggttatcg atcacaacgt
     1741 gggggtgggc gtcattaccg atggtcatct gctacacgca ggcagcagta gtctcgtgga
     1801 aataggccac acacaggtcg acccatatgg taaacgctgt tattgcggga accacggctg
     1861 cctcgaaacc atcgccagtg tggacagtat tcttgagctg gcacagctgc gtctcaatca
     1921 atccatgagc tcgatgttac atgggcaacc gttaaccgtg gactcattgt gtcaggcggc
     1981 attgcgcggc gatctactgg caaaagacat cattatcggg gtgggcgcgc atgtagggcg
     2041 cattcttgcc atcatggtga atttatttaa cccacaaaaa atactgattg gctcaccgtt
     2101 aagtaaagcg gcagatatcc tcttcccggt catctcagac agcatccgtc agcaggccct
     2161 tcctgcgtat agtcagcaca ttagcgttga gagtactcag ttttctaacc agggcacgat
     2221 ggcaggcgct gcactggtaa aagacgcgat gtataacggt tctttgttga ttcgtctgtt
     2281 gcagggttaa cattttttaa ctgttctacc aaaatttgcg ctatctcaat ttgggccagg
     2341 aaagcataac ttagactttc aaggttaatt attttcctag tttatatttg tgaagcataa
     2401 cggtggagtt agtgatgctg aagcgtttct ttattaccgg tacagacact tctgtaggga
     2461 aaacggtggt ttcccgcgca ttgctacaag cgttagcctc ccagggaaaa agggttgcgg
     2521 gatataaacc cgtagcgaag gggagcaaag agacacccga agggctgcgt aataaagatg
     2581 ccttggtgtt gcagagtgtt tcaaccatcg aactgcctta tgaagcagtt aatcctatcg
     2641 cgttaagcga agaagaaagt agcgtggcgc acagttgccc aatcaattac accctcattt
     2701 caaacggcct ggcaaacctg accgataaag tcgatcatgt cgtggtagaa gggactggcg
     2761 gctggcgcag tctgatgaat gatttgcgtc cactctctga atgggtagtg caggaacaac
     2821 tgccggtgtt gatggttgtc ggtattcagg aaggttgcat taaccatgca ctgctaacag
     2881 ctcaggcgat cgccaacgac gggctgccgc tcattggctg ggtggctaac cgaatcaacc
     2941 caggactggc gcattatgcg gaaatcattg atgtgctggg taaaaaactt ccggcaccgc
     3001 tcattggtga actgccttat ctgccgcgcg ccgaacagcg tgaactgggg caatacatcc
     3061 gcttagctat gctgcgcagt gtgctggcgg tagatagagt cacggtgtaa cgtccgcgaa
     3121 attaccgacg caattacgca ggcaatcaat aaaccgggga gtagctgata ctccccggtc
     3181 atttcacata tcatcaacgt cgacataatc ggcgcgtgcg tggttgccgc caacagtgtc
     3241 gccatcccgg tcaatccgag taaaagcgta atttcttcgc catcagggaa ccataatccc
     3301 aggctacgac catacaacat gccaatggca agaccgataa atagcgtcgg tgtaaagaca
     3361 ccaccgggtg cgccggaacc gctactcgcc agcacggcac acagtttaca aaggaagatc
     3421 ccggcaatga tcattaacag tggtggggcg gttaaaaagg attgtacggt gctatagccg
     3481 ttgccccaca ctgcaggtgt gaacagggaa agcagaccca cgatcaaccc gcctagtgcc
     3541 agttgccagg gcggcgcaag tttgagactc acaaatccac gatgacaggc gttcattaac
     3601 gttaacaaca gtggtccgca cagacctgcc agcacacctg tactgataat cagcgcatag
     3661 tcacgagcct gaaccgtcac tgagagttgt acgttgtaga gtaacgcgtc gctatgatta
     3721 atcagattgc taaccagcca tgccacgatg gcggaaataa tcaccgggcc gagagaggcc
     3781 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     3841 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     3901 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     3961 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     4021 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     4081 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     4141 ccgagcgccg gagttagcag ccgtcgccag gaggaaaggt ttgtcgctgc attgaccaga
     4201 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     4261 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     4321 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     4381 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     4441 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     4501 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     4561 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     4621 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     4681 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     4741 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     4801 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     4861 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     4921 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     4981 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     5041 cttccgcact ctctggagcg taataaaaca gcgcgcccaa cacgcgcgcc gcgacagaaa
     5101 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     5161 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     5221 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     5281 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     5341 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     5401 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     5461 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     5521 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     5581 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     5641 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     5701 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     5761 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     5821 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     5881 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     5941 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     6001 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     6061 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     6121 tgcgcgcgcg gcagtggcgc gacggcagcc agagtgccgt gcttctggcg cagctcttca
     6181 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     6241 tcggcgacgc gcgaataaca gccatcgcac ttcgtcatgt gccctttttc agcattgtac
     6301 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     6361 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     6421 catgcggggt catcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     6481 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     6541 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     6601 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     6661 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgccagcg gtgaagggcg
     6721 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     6781 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     6841 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     6901 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     6961 cacatcaata ttgccgtagc tggagtgggt acgcgctttg gtttggcgac ttcactcagc
     7021 atccagtaga tgggtttgcg ttcaaatttt gctgaggttg cgggttgggc gaggatcaca
     7081 tagcccatat tccctgcaga ttcatgagag ataaggtctt cttgctctgt tggcatcagg
     7141 tcaggcaaca ggatatcgca atacttagcc gaggccgtca tgaagtggtc aatgccaaca
     7201 atcatctcgc acttgctgtc atcctgaagc acctcatggg tgtgattgat gtcgccatgt
     7261 tgattgatca atgtgttact ggcgtagcac cataaaaact tgatggggac atccagtttt
     7321 tcttttccac gaacaccatc acgggtcgcg gtcattttcg taccatggtc gatggcatct
     7381 gtccatgtaa aaacggaaat ctgcgtttta acaggattct cgagcatcgg gaaccattct
     7441 acccccagat cccagctacc ttcgcgtaca cctgagttgc cgccgtttat gccgacgtta
     7501 ccggtaagaa cggaaagcat ggcaatagcg cgggacgttt gttcgccgtt ggagtgtcgt
     7561 tgtggccccc aaccctgaca aatataagca ggttttgctg aaccgatctc tcgtgccaac
     7621 tggataattt tttctgccgg gatgctggtg atttttgctg cccattccgg cgttttagct
     7681 atgccgtcag gcccttcgcc cagaatatag gctttataat gcgcgttacg tggtgcgttg
     7741 gcgggcagcg ttttttcatc gtaaccaaca caatatttgt cgagaaatgg ctgatcgacc
     7801 atgttttcag taatcagtac ccaggcaatc gcacaggcca gtgcgccatc ggtgccaggg
     7861 cgaatgggca gccattcatc ttcacgcccg gcagccattc atcttcacgc ccggcagctg
     7921 tgtcgttata gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct
     7981 cgacgtagta agtgacacca ccgccgctca tccgcgtttc tgccgggtta tttccgaaca
     8041 taacgaccag tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca
     8101 tataactcat tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac
     8161 ccccgcaaga gttcatcaga cggtacggga cgttcgagtt ggtgatattt ccgccatcta
     8221 cgcctgttcc gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa
     8281 gattatcact gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac
     8341 cgcgctcgcc gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc
     8401 ggatagagcg cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg
     8461 tcgtatcaga ctccacccag tacacggtgt catctttcac atgcaaacgt agcagacagc
     8521 ggctcccgca gttaacggtg caggaactcc agaccgtttt ctcttctacc ggagcctctg
     8581 ccgcccggac catttgggaa aatggcagag tgaaagcact gcttgccagc gcgagactgc
     8641 caagtgcgga ggttttcatc agacttctat ggctgatttc agccttcatg agcgcctctg
     8701 tggtatggat tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat
     8761 ttatggttgt agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc
     8821 ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga
     8881 ttttatcgcc agacatattg ggctcgtgcc aggctccctg gcccatagcg ctaactccag
     8941 ggagaatgcg tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc
     9001 gcaccatatc gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacacct
     9061 cctgacggca ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt
     9121 tgtaatggaa accaaacagt tgcagtggga aggttctacg ttcaggggag tcccagcctt
     9181 caaaggttga ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg
     9241 tacgggcaat ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa
     9301 gtggatttgc ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat
     9361 ctttacgctt atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt
     9421 tggcaagcat tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttctgtga
     9481 acttttgata gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg
     9541 gtttacgttc gaatttttcg ctggtgactg gctggaggaa aatgagatat cccatgttac
     9601 cggcgtagtc gttaggaata atatcttcct gctcaacggt catcaggtct gccagcagaa
     9661 tgtcggcata ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt
     9721 tcgattcgtc ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg
     9781 tatttcccgc gtagttccag atggactcca ctgtcagacg gatggacgga cttgaagtag
     9841 ttgactcaac gctacttaag gtcctgatat gactgacatt aaccatgacg atactgcata
     9901 tccatccagt tctttctctg ggtctcccta tctgtatcag caagtctaca cctcaagtcc
     9961 tgacacaagg ctgacacaaa tcaaggacct tgctacgcca cagctagtcc ggagaaccaa
    10021 cgggcgttac actgtccgct tcagacttaa aggagctacc agcccgtttc tatcagtctc
    10081 aacccgcagc acagaccgga ggaccgctac aatgcgtcag agagagctcg cagccacagc
    10141 taaggcattc ctactggaca atcctgaagt cacactacag gagcttacag cgcacatacg
    10201 gtccatggct gagcagttcc tgactgatgc cagtgatgac tactggaatg gcgtagaggt
    10261 ggcgacactg gttgacgaga agtccaacct cagggaactt gcagcgacaa gagcgttgag
    10321 tgtggaccag cagaggggca ttaagttagc gctggaagtc ctgaccgcag cacagcagag
    10381 agtcgacact ggtgatacct ctggtctgat taagctgatt gatggtggcg taagccagct
    10441 tactgatgat aataaccata ctgattactc aactataggc gactctacgt cgattctgaa
    10501 gaatgagcag ggggatagac cagcagtttt cacacaggaa cgtcagttac tcactccaga
    10561 gtcacgagaa tcatctgtac tttttcagga attggtgaca tcactgctgg ctgagaaggt
    10621 ccagacactg aaacaatcca gctttaagga cttgtcagcg tcc
//
LOCUS       DAECOD010000123        10615 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.123, whole genome shotgun sequence.
ACCESSION   DAECOD010000123 DAECOD010000000
VERSION     DAECOD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.123"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="KKO15_003386"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="KKO15_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBD6362055.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="KKO15_003387"
     CDS             1207..1869
                     /locus_tag="KKO15_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6362056.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="KKO15_003388"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="KKO15_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBD6362057.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="KKO15_003389"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="KKO15_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="KKO15_003390"
     CDS             2795..3100
                     /locus_tag="KKO15_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD6362058.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="KKO15_003391"
     CDS             3103..3507
                     /locus_tag="KKO15_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBD6362059.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="KKO15_003392"
     CDS             3497..3796
                     /locus_tag="KKO15_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBD6362060.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="KKO15_003393"
     CDS             3982..4278
                     /locus_tag="KKO15_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD6362061.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="KKO15_003394"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="KKO15_003394"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBD6362062.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="KKO15_003395"
     CDS             5627..5995
                     /locus_tag="KKO15_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD6362063.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="KKO15_003396"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="KKO15_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBD6362064.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="KKO15_003397"
     CDS             7203..7904
                     /locus_tag="KKO15_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBD6362065.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="KKO15_003398"
     CDS             7927..8091
                     /locus_tag="KKO15_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362066.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="KKO15_003399"
     CDS             complement(8303..9613)
                     /locus_tag="KKO15_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBD6362067.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..>10615)
                     /locus_tag="KKO15_003400"
     CDS             complement(9641..>10615)
                     /locus_tag="KKO15_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362068.1"
                     /translation="FVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGLVLI
                     CILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLVKNA
                     IITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFFYAV
                     SFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYL
                     GFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICS
                     PIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         2687 a         2789 c         2632 g         2507 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa tatattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaa
//
LOCUS       DAECOD010000124        10564 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.124, whole genome shotgun sequence.
ACCESSION   DAECOD010000124 DAECOD010000000
VERSION     DAECOD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.124"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="KKO15_003401"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="KKO15_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBD6362069.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="KKO15_003402"
     CDS             complement(2005..2928)
                     /locus_tag="KKO15_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6362070.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="KKO15_003403"
     CDS             3145..4488
                     /locus_tag="KKO15_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6362071.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="KKO15_003404"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="KKO15_003404"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBD6362072.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="KKO15_003405"
     CDS             6508..6732
                     /locus_tag="KKO15_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBD6362073.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="KKO15_003406"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="KKO15_003406"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBD6362074.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8293)
                     /locus_tag="KKO15_003407"
                     /pseudo
     CDS             complement(7326..8293)
                     /locus_tag="KKO15_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8417..9409
                     /gene="tehA"
                     /locus_tag="KKO15_003408"
     CDS             8417..9409
                     /gene="tehA"
                     /locus_tag="KKO15_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBD6362075.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9406..9999
                     /gene="tehB"
                     /locus_tag="KKO15_003409"
     CDS             9406..9999
                     /gene="tehB"
                     /locus_tag="KKO15_003409"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBD6362076.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10302..>10564
                     /locus_tag="KKO15_003410"
     CDS             10302..>10564
                     /locus_tag="KKO15_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HBD6362077.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQ"
BASE COUNT         2717 a         2471 c         2654 g         2722 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaata attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 taatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgctatc acatcgttaa aatcgctaat ggcgataatc tgccgtaata
     8221 acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttaggc
     8281 gatatttgcg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
     8341 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
     8401 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
     8461 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
     8521 ggtcagccac tggttagggg atgggctggt gattctggcg atgatcatct ggggattatt
     8581 gactagcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cggaagttcg
     8641 ccatccagtg ctgagcagtt ttgttagttt gtttccggca acgacgatgc tggtggcgat
     8701 tggttttgtt ccgtggtttc gcccactggc ggtgtgcatg ttcagttttg gtgtcgtggt
     8761 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgcggatctc accctgaaga
     8821 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
     8881 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
     8941 atggctaagc cttgaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac
     9001 ggcactgcgg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
     9061 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
     9121 gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc
     9181 ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg
     9241 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
     9301 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
     9361 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgac
     9421 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt actggaagcg
     9481 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
     9541 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
     9601 gccaacgtcg agcgcattaa atccattgaa aatctggata atttacacac ccgggtcgtt
     9661 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
     9721 atgttccttg aggctaaaac catcccaggg ttgattgcca atatgcaacg ttgcactaaa
     9781 cctggtggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
     9841 ggcttcacgt ttgccttcaa agagggagaa ttacgtcgat attacgaagg ctgggagagg
     9901 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
     9961 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgac ccggtaagca caaaacgcgt
    10021 gaaaattccc cacgctgaga tgatttactg ttcttctttt cggtaagcat attttttatc
    10081 gaagggatgt gaaattaatc acagtagtcg aagtttttta gcagcttaac ttactgaaat
    10141 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
    10201 gctgaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
    10261 tataacttag aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
    10321 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
    10381 agccagataa atattctggt tttttaaaca attactctga tttaaaagaa acaacctcag
    10441 caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc aaatatgaca
    10501 gcatcgtctg gaacccaatc acttattatc cggtaccaaa accttctacc caggtagggc
    10561 agaa
//
LOCUS       DAECOD010000125         9924 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.125, whole genome shotgun sequence.
ACCESSION   DAECOD010000125 DAECOD010000000
VERSION     DAECOD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.125"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..712
                     /locus_tag="KKO15_003411"
                     /pseudo
     CDS             1..712
                     /locus_tag="KKO15_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            725..1638
                     /locus_tag="KKO15_003412"
                     /pseudo
     CDS             725..1638
                     /locus_tag="KKO15_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2317..2535
                     /locus_tag="KKO15_003413"
     CDS             2317..2535
                     /locus_tag="KKO15_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBD6362078.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2753..2995
                     /locus_tag="KKO15_003414"
     CDS             2753..2995
                     /locus_tag="KKO15_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362079.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3325..4254)
                     /gene="fdhE"
                     /locus_tag="KKO15_003415"
     CDS             complement(3325..4254)
                     /gene="fdhE"
                     /locus_tag="KKO15_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBD6362080.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4251..4886)
                     /gene="fdoI"
                     /locus_tag="KKO15_003416"
     CDS             complement(4251..4886)
                     /gene="fdoI"
                     /locus_tag="KKO15_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD6362081.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4883..5785)
                     /gene="fdoH"
                     /locus_tag="KKO15_003417"
     CDS             complement(4883..5785)
                     /gene="fdoH"
                     /locus_tag="KKO15_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBD6362082.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5798..8848)
                     /gene="fdnG"
                     /locus_tag="KKO15_003418"
     CDS             complement(5798..8848)
                     /gene="fdnG"
                     /locus_tag="KKO15_003418"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8261..8263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD6362083.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9042..9875
                     /gene="fdhD"
                     /locus_tag="KKO15_003419"
     CDS             9042..9875
                     /gene="fdhD"
                     /locus_tag="KKO15_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBD6362084.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2542 g         2497 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag ttttttgggc agtgaaaaac cgttgtttgt aaagcatacc
      901 gcaatgatga gggaggcatg taaggaatta ccgcagtcag tggaatatgt tcctttagcc
      961 tgtgaagcgc atagccatcg cagcgtcgcc cttgccaaaa ccgtggctgc gcccatcatg
     1021 gcgacaggtg tgggcttggc attaatgtat gcaacatcat caggaccgta ttatgcgcaa
     1081 agtctttctg gttcgccgga tatcgagatg ataaaaaatc gcatggctga attgtttaac
     1141 cagtcggata tttctgtgcg caacaaatat agcccggctt ttgataaatg gaatgaactg
     1201 gtagataaat taaatcacga aagaaataca gttccgtttg cgtgtttgac caccatttta
     1261 gcgacggcga taaatgaaac tcttgagggg gatacgaatg ttgccgttgt gatggggtgt
     1321 aaatctgcga aagatcgcac gatatccatt gtccttggca actcgatgtt gcaaacactc
     1381 cttgaaaaac gcctcgccga tagtggagaa atagaaaatc tttttgatca acagggatat
     1441 tttagttgcg attcattaac acccgaagaa ttaatgatgt taaaagacct ttttgatatt
     1501 cgagttcttc atgtctctaa caaattcaat gttggattgc aaggtaatat caacactgat
     1561 gttttacaag attcgttttt taaaaatgtc gatttcattc atgaatataa ctcgtatacc
     1621 gtagggatgg gagtctgacg agtaaaactc attaggatta tcagaattcg gacaatgcat
     1681 tgcaaaaatg acatacaaag agagatgtgt gcaggtactt agccgcgagg ggcatggcga
     1741 agccatacag aaagtaccag tattcagaac tccagtgaaa gcggcatagt ggccgcctgc
     1801 tcaggagttt tgctatgacc cgtaatcgtg ctgaacgtcg ccaccctatg atgcgattaa
     1861 aaaaactcat cgtcgcgctg atgattgacg tcaatgctat cggatgatgc cgggggtgtt
     1921 ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga tgagcaagta
     1981 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
     2041 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
     2101 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
     2161 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
     2221 aacaaaatga catgcaaaaa tccgcatttt tttaaatgct tttccggcgt aattttcgca
     2281 catacattaa tgagtaatat atgtaaggtg gtggtaatgg cgatgaatac ggtttttctt
     2341 catttatcag aagaggcaat taaacggctg aacaagcttc ggggctggcg taaggtttca
     2401 cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca atttccggtg
     2461 cgtaaagcaa agggaggcag gcaaaagggg gaggtagttg gtgttgatga tcagtgtaag
     2521 gagcataagg aatgaaaaaa atttttcatt cttgaatata aaaaacagat gcccttattc
     2581 tggtattaat acaaggctgt tttacttgaa cttataataa ctgcaactgt tacatcgtat
     2641 ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt taaagattcg
     2701 gcaagggtac ttttctctcc atagattcaa aaatgatatt ggcgagatat ttatgaactc
     2761 tctggcaggt atagatatgg gcagaatttt actcgattta tcgaatgagg tgattaagca
     2821 acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa gggaagcggt
     2881 agatcaatac ctgataaatc aatcgcaaac agcaagaacc agtgttcctg gcatctggca
     2941 agggtgtgag gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat ggtaatggga
     3001 aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg atgcggcgtg
     3061 aacgccttat ccggcctaca aagtcatgca aattcaatat attgaagttt attggtaggc
     3121 ctgataagcg cagcgcatca ggcaatcata cgcttgtcat cagtgacata cccataacct
     3181 tatcgatgtg ttggagggaa gaacgaggca aacagacaat tgcggcatga tcttgaatgg
     3241 gtaacgcgga atacgagaga tggtgtcgtc acaccctatg agctgtaatc cgggcgacct
     3301 gcgccacccg gaaaacgtca gagattactc tccttcaccc ggaaacagga acgggttaat
     3361 ggaactacgg gcgtagcctt cttgctccat tcgcgcgtcc agtaccagag aggcgaggtc
     3421 atctgccacg gcttcaactt tcggttcttt ttcctgataa agaatcttca ggtaagtgcc
     3481 gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg accagtaatg
     3541 cagtttgccg ctctgctcgc agttgctgca ttttacgcgc actacgtgcc attcggtttc
     3601 acacaggttg cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca ccatgctgga
     3661 caccggcata gagccacata ccgggcaata ttgacgttgt tcgccgtatt cagcgcgggc
     3721 tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagcgcag cccagataaa
     3781 cggtgcttta tcgctgctga cggacgagaa atcagaggca aacagcgcgc tggccatatc
     3841 ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg ccgggccgct
     3901 catttcaggt ttcagctcag caattagcgc catcagcagc ttttgccagt gcttatcacg
     3961 cggcagaacg tgaatatcca gcgggggctt gccctgtgcg ctggcttctt taatgcgcgc
     4021 ggtcagatcc atctccagcg gatggtcgta cagcaccact ttctgggcgt gggcgataag
     4081 cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc gcagacgctc
     4141 ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa tcatatccgc
     4201 cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac tcattcagct
     4261 ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt cttcgcccag
     4321 gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca aagggcggcg
     4381 tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg cagcattaac
     4441 gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca gataatcacg
     4501 ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca tttctgaccg
     4561 aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg aatattcttc
     4621 gcccaaaaga tatcatcccg attgattagg ttttgatgcc agtaacggaa aaacatgatg
     4681 atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc cagctgcggt
     4741 gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag cccgctcacc
     4801 gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg cgcggtgtag
     4861 cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg tgcagattat
     4921 tctcttcctc atccgcacgg ttcggaccga cacccacgta gtggaagata ctggccgcaa
     4981 aggtagccgc aaagccaaca gccgcgagcg gtttccagat gcctttccag aatttcacgg
     5041 tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg tcagcatggt
     5101 gcagcacgta catgacgtgt gtaccaccga cgcctgccgg atcgtacaga cccgcattgt
     5161 cgtaaccgcg ggttttcagc tcagcaacgc gctcgctcgc cagcgttttc atcgactctt
     5221 tcgtgccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt tcttgcccaa
     5281 ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc gggttgaggc
     5341 gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac tgctcggact
     5401 ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg cacgctttca
     5461 ggcagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat tccagtttgt
     5521 cgttctgctc cacttccgag aagcgcatta ccgtccacga tttggcgctt aaatcattgg
     5581 ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc cactctgaac
     5641 acgccacctg acaggcttta cagccgatac aggtggtaac gtcgatgagt ttcgccactt
     5701 cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg gaacgacgaa
     5761 tgatatcttg agattgataa gccatatgtc gtctccgtta caccttttcc acattcacaa
     5821 ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc gttaaagtat
     5881 tggcaataaa gcctttttta gctacgcctt catagcccca gtgaatcgga ataccgatgg
     5941 tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc accgctttgg
     6001 ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca atgccaagtt
     6061 tattcgccag cgactccccg atttccacaa actgctctgg ctgcaaaatc gcgttcaaca
     6121 gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt ccgacatacg
     6181 ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt gcagctgggt
     6241 tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac ggctcgtagt
     6301 gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc atgccttcct
     6361 gctgcatgat aaacggcccg acgccgctac ccggaggcgc tgcgctgtag tccggaatat
     6421 cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga tcccacgggt
     6481 taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc ggccatgccc
     6541 atgcccagcc cagcgtgtta ccgaggccag acggatcggc gttatcacga cgcgccatct
     6601 ggttgccttc cggcgtccag ctaccggcga aaatccagca gccacaggag gttgtaccgt
     6661 catcgcgcaa ttgggcgaac gagctaagtt gttggccttt cttgacgata accgcaccgg
     6721 ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgct acttcttccg
     6781 acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctga tccgggttcg
     6841 cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag aggatctcac
     6901 cgtcagtcag cgcaatcccc ggggcgtccg cacctttcca gtgccactgc aaccagcgac
     6961 ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga cggaacactt
     7021 cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc cagaagttag
     7081 aggtttcagt gttcagtggg tcgatggtga cgaggaactt cagtttcgac agacaaccga
     7141 tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag atatagccat
     7201 tgaccttgcc ctctttcatc atctcgaagt attgcaggac gtcgtagcct ttatcccact
     7261 tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc tttatcacca aagaaggcct
     7321 tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttacctgg ccttccagca
     7381 gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc tcgcttggca
     7441 gcgtcatgta acctggcaga ctctgcgaca gtagccccag gtccgtcagc ccctgaatat
     7501 tggagtgacc gcgcagggcg ttaacgccgc cgccggccat ccccatattg ccgagcagca
     7561 gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc gtccagccga
     7621 gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg tattcgcaga
     7681 ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct ggcgtgtagc
     7741 gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc gtggtatcgc
     7801 gtttggcgaa gccgttttcg tccagttcat aagtccagga ggatttatcg tacttgcgtt
     7861 tttccgcgtc gtagccggtg aacaggccat cttcaaagcc gtaatcctca cgcacgatca
     7921 ggctggcgtt ggtataggct tcggtgtatt cgtggttgaa tttttcattg ttcagcaggt
     7981 acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg gcatagtagt
     8041 cagctaccgc cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc gcgccgttgt
     8101 gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg gcgttaccgc
     8161 ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc atcgcaccgc
     8221 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc gcctggttgt
     8281 cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg gtttcgttac
     8341 tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc acgccttcgg
     8401 cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg atgcgatcaa
     8461 acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg tattccggaa
     8521 acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc ggacaaagtg
     8581 caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat gctttggcgt
     8641 tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa caataggtgc
     8701 aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc gcgagtgcta
     8761 cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca gcgcagatct
     8821 taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat tgtcacgtcc
     8881 catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt gcggaagggg
     8941 ccgcaaaggt ccagaattgg ctcaatttcc ctccatctag gaagggtttg taacagaata
     9001 ccataatgtt ggtgtgtatg ttcttatctg gttaagagaa agtgaaaaaa acacagcgaa
     9061 aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta tggcggcgtg
     9121 atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt gcgctggtct
     9181 acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag tactttgcgc
     9241 tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc ggcatggatg
     9301 tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc cgctttatgg
     9361 ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta tgcggcgtgg
     9421 agcaacttaa tgacatcgga aaaccggtgc agccgctacc gttcacccag acgtttgatc
     9481 tcaacaaact ggatgatgca ttacgtcatc tcaacgattt tcagccagtg gggcgactga
     9541 ctggttgtac tcacgccgct gcctggatgt tgccatctgg cgaactggtc ggcgggcatg
     9601 aagacgtggg tcgccatgtg gcgctggaca aactgttagg ccgccggtca caagaagggg
     9661 aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa atggtgcaaa
     9721 agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc acgcttgctg
     9781 tagaagtggc tgagcgctgt aatctgacac tggtaggttt ttgtaaaccg ggtcgggcaa
     9841 cggtttatac ccatccgcag cgtttgagca attaattaaa ataagtcctg cgatatatat
     9901 taaattgcag gacttattca tttc
//
LOCUS       DAECOD010000126         9857 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.126, whole genome shotgun sequence.
ACCESSION   DAECOD010000126 DAECOD010000000
VERSION     DAECOD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.126"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            383..1501
                     /locus_tag="KKO15_003420"
     CDS             383..1501
                     /locus_tag="KKO15_003420"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD6362085.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="KKO15_003421"
     CDS             1567..1815
                     /locus_tag="KKO15_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBD6362086.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="KKO15_003422"
     CDS             1880..2248
                     /locus_tag="KKO15_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD6362087.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="KKO15_003423"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="KKO15_003423"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBD6362088.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="KKO15_003424"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="KKO15_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBD6362089.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="KKO15_003425"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="KKO15_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBD6362090.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="KKO15_003426"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="KKO15_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBD6362091.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="KKO15_003427"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="KKO15_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2416 a         2725 c         2386 g         2330 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgct
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatactagt ttttagactt
     9841 gtcattggag aacagat
//
LOCUS       DAECOD010000127         9784 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.127, whole genome shotgun sequence.
ACCESSION   DAECOD010000127 DAECOD010000000
VERSION     DAECOD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.127"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(37..381)
                     /locus_tag="KKO15_003428"
     CDS             complement(37..381)
                     /locus_tag="KKO15_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD6362092.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            513..2423
                     /locus_tag="KKO15_003429"
     CDS             513..2423
                     /locus_tag="KKO15_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBD6362093.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2481..3176
                     /gene="tsaB"
                     /locus_tag="KKO15_003430"
     CDS             2481..3176
                     /gene="tsaB"
                     /locus_tag="KKO15_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBD6362094.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3216..3797
                     /gene="yeaY"
                     /locus_tag="KKO15_003431"
     CDS             3216..3797
                     /gene="yeaY"
                     /locus_tag="KKO15_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBD6362095.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4002..5687
                     /gene="fadD"
                     /locus_tag="KKO15_003432"
     CDS             4002..5687
                     /gene="fadD"
                     /locus_tag="KKO15_003432"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBD6362096.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPELVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5769..6884
                     /gene="rnd"
                     /locus_tag="KKO15_003433"
     CDS             5769..6884
                     /gene="rnd"
                     /locus_tag="KKO15_003433"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBD6362097.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6938..7903)
                     /gene="yeaX"
                     /locus_tag="KKO15_003434"
     CDS             complement(6938..7903)
                     /gene="yeaX"
                     /locus_tag="KKO15_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBD6362098.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7959..8576)
                     /gene="yeaW"
                     /locus_tag="KKO15_003435"
                     /pseudo
     CDS             complement(7959..8576)
                     /gene="yeaW"
                     /locus_tag="KKO15_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(8918..9484)
                     /locus_tag="KKO15_003436"
     CDS             complement(8918..9484)
                     /locus_tag="KKO15_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6362099.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9696..>9784
                     /locus_tag="KKO15_003437"
     CDS             9696..>9784
                     /locus_tag="KKO15_003437"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6362100.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2370 a         2406 c         2576 g         2432 t
ORIGIN      
        1 taaattatcg tctgacttat tttgcttcga taaagcttac accgcagcca caatcttaat
       61 ttcaacttta tacttcgggt tcatcaaacc cgcttgtacc gtgcagcgca ccggcgcatg
      121 acccgcgaca acccaggcat cccacgcttt attcatcgcc gcgaagtcgt ttttatcggc
      181 caggaaaatg gtggcatcca gaatgctcga tttattgctg ccctgttttt ccagcacggc
      241 gtcaatctgt gccagcgtgt tggcggtttg ctcaaaggca tcggcgtcga ggttttccgg
      301 tacaccagtg tagtagagcg tgttgttgtg gattactaca tcagaccagc gggcttcagc
      361 atcgatacga acgatagtca tcattttgtc ctcattatac ttccattttc gtgctgcaaa
      421 gactgccaca atgtgaggtt gccgtcacca cttcaactgg cgaaagcgcc ctcatcctga
      481 cataatccct gttcaaatca acagggggta gtgtgacgga cgattttgca ccagacggtc
      541 agctggcgaa agcgatacca ggctttaagc cgcgagaacc acagcgacag atggcggtag
      601 ccgtcaccca ggcgatagaa aaaggccagc cgctggtggt ggaagcagga accggtacgg
      661 gcaaaaccta cgcttacctg gctcctgcgc tgcgggcgaa aaagaaagtc attatctcga
      721 ccggctcaaa agcgttgcag gatcagctct acagccgcga tttgccaaca gtctcaaagg
      781 cattgaaata tacgggcaac gtggcgctgc tgaaagggcg ctcaaactac ctctgcctcg
      841 aacgtctcga acagcaggcg ctggcggggg gcgatctgcc ggtacaaatc ttaagcgatg
      901 tgatcctgct gcgctcctgg tctaatcaaa cagtcgatgg tgatatcagc acctgcgtca
      961 gcgtggcgga agattcacag gcgtggccgc tggtcaccag caccaacgac aactgtcttg
     1021 gcagcgactg cccgatgtat aaagattgct ttgtggtcaa agcacgtaaa aaagcgatgg
     1081 acgccgatgt ggtggtggta aaccatcatc tctttctggc ggatatggtg gttaaagaga
     1141 gtggatttgg cgagttgatc cctgaagcgg acgtcatgat cttcgacgaa gcccaccagc
     1201 taccggacat tgccagccag tattttggtc agtcactctc cagtcgacaa ctgctcgacc
     1261 tggcaaaaga catcaccatc gcctaccgca ccgaattaaa agacacccag cagttacaaa
     1321 agtgcgctga tcgtcttgcc cagagtgcgc aggattttcg tctgcaactc ggtgagccag
     1381 gttatcgcgg taacctgcgt gagctgttag ctaatccgca aattcagcgg gcatttttac
     1441 tgctcgatga caccttggaa ctttgttatg atgtggcgaa actgtcactg gggcgttccg
     1501 ccttgctgga tgcggcattt gagcgcgcca cgttgtatcg cacacggctg aagcggctaa
     1561 aagagatcaa tcagccgggc tacagctact ggtacgaatg cacttcgcgc cattttactc
     1621 tggctctcac gccgctcagc gtggcggata aattcaaaga gttaatggcg caaaagcccg
     1681 gtagctggat cttcacctca gcaacgctgt cggtgaacga cgatctgcat catttcacct
     1741 cgcggcttgg gatagaacag gcggagtcgt tgctattgcc aagcccgttt gattacagcc
     1801 gccaggcgtt actctgtgtg ccgcgcaatc tgccgcaaac caaccagcca ggttctgctc
     1861 gccagttagc ggcaatgctg cgaccgatca tcgaagctaa caacggtcgt tgttttatgc
     1921 tttgtacctc gcacgccatg atgcgcgatc tggccgagca gttccgcgct accatgacgc
     1981 ttcccgtttt gttgcagggg gaaaccagta aaggccaact gttgcagcag tttgtcagtg
     2041 ccggtaatgc gcttcttgtg gcaaccagta gtttctggga aggggtggac gtgcgtggcg
     2101 atacattgtc attggtaatt atcgacaaat tgccgtttac ctcgccggat gatccactgt
     2161 taaaagcgcg catggaagat tgtcgtttgc gcggtggcga cccgttcgat gaagtgcaac
     2221 taccagatgc cgtcattact ctcaaacagg gggtaggtcg actgattcgc gacgccgacg
     2281 atcgtggcgt gctggtgatt tgtgactatc ggctggtgat gcgtccttac ggcgcgacgt
     2341 ttctcgccag tctgccgccc gcgccacgca cccgtgacat tgcccgtgcg gttcgtttcc
     2401 ttgcgatacc atcctccagg taatttgtta cggagtatgg tatgatgcgc gcctttttat
     2461 tgatctaagc acgaagaccc atgcgaattc tggctatcga taccgcgaca gaggcctgct
     2521 ctgtcgccct gtggaacgac ggtactgtca acgctcattt tgagctttgc cctcgtgaac
     2581 atactcaacg aatcttaccg atggtgcagg atatcctgac caccagcgga acttccctga
     2641 ctgatattaa cgctctggct tacgggcgcg gccccggtag ctttaccggc gtgcgcattg
     2701 gtattggcat cgcgcaaggg ctggcgcttg gcgcggaatt accgatgatt ggcgtctcca
     2761 cgctaatgac gatggcgcaa ggggcgtggc gcaaaaacgg cgcaacacgc gtgctgtcag
     2821 ccattgacgc gcgaatgggc gaagtttact gggccgaata tcagcgtgat gaaaacggta
     2881 tctggcacgg tgaagaaacc gaagccgtac tcaaacccga actcgttcat gaacgaatgc
     2941 aacagctttc cggcgaatgg gtgacggtag ggacgggctg gcaagcctgg ccggatctcg
     3001 gtaaagagag cgggctggtt ttgcgcgatg gcgaagtgtt actccccgct gcagaagata
     3061 tgctgccgat tgcttgtcag atgtttgccg agggtaaaac ggtggcggtg gaacatgccg
     3121 agccggttta tttgcgtaac aacgtcgcat ggaagaaact tccgggcaaa gaatgaatct
     3181 taagagtatg ccctgagaaa aaaaggagtc gcatcatggc ggttcaaaag aatgttatca
     3241 aaggcatact ggcaggtacg tttgcgctaa tgctgagcgg ttgtgtcact gtgccggacg
     3301 ccattaaagg cagcagtccc acgccgcaac aagatttagt tcgggtgatg agtgcgccgc
     3361 agctgtacgt tggtcaggag gcacgctttg gtggcaaagt ggttgcggta caaaaccagc
     3421 aagggaaaac ccgcctggaa attgctaccg taccgctgga cagcggagct aggccgacgc
     3481 tgggagaacc ttctcgcggt cgcatttatg ccgatgtgaa cggttttctg gacccggtgg
     3541 atttccgtgg acaactggtt acggtagtcg ggccaatcac cggtgcggtt gacggcaaaa
     3601 tcggcaatac gccctataaa tttatggtga tgcaagtaac gggttacaaa cgttggcatt
     3661 taacccagca ggtgattatg ccgcctcagc cgattgatcc atggttttat ggcggtcgtg
     3721 gctggcccta tggctacggc ggatggggct ggtataatcc cggccccgcg agagtacaaa
     3781 cagttgtaac cgaataattg cttgttttta aagaaaaaga aacagcggct ggtccgctgt
     3841 ttctgcattc ttacggtaaa gataaaaata aatagtgacg cgcttcgcaa ccttttcgtt
     3901 gggtaattat caagctggta tgatgagtta atattatgtt aacagcgtgt atatcatttg
     3961 gggttgcgat gacgacgaac acgcatttta gaggtgaaga attgaagaag gtttggctta
     4021 accgttatcc cgcggacgtt ccgacggaga tcaaccctga ccgttatcaa tctctggtag
     4081 atatgtttga gcagtcggtc gcgcgctacg ccgatcagcc tgcgtttgtg aatatggggg
     4141 aggtgatgac cttccgcaag ctggaagaac gcagtcgcgc gtttgccgct tatttgcaac
     4201 aagggttggg gctgaagaaa ggcgatcgcg ttgcgttgat gatgcctaac ttattgcaat
     4261 atccggtggc gctatttggc attttgcgtg ccgggataat cgtcgtaaac gttaacccgt
     4321 tgtatacccc gcgtgagctt gagcatcagc ttaacgatag cggcgcatcg gcgattgtta
     4381 tcgtgtctaa ctttgcccac actctggaaa aagtggttga taaaaccgcc gttcagcacg
     4441 taattctgac ccgtatgggc gatcagctat ctacggcaaa aggcacggta gtcaatttcg
     4501 ttgttaaata catcaagcgc ttggtgccga aataccatct gccagatgcc atttcatttc
     4561 gtagcgcact gcataacggc taccggatgc agtacgtcaa acccgaactg gtgccggaag
     4621 atttagcctt cctgcaatac accggcggca ccacaggtgt ggcgaaaggc gcgatgctga
     4681 ctcaccgcaa tatgctggcg aacctggaac aggtcaacgc cacctatggg ccgttgctgc
     4741 atccaggtaa agaactggta gtaacggcac tgccgctgta tcacattttt gccctgacca
     4801 ttaactgcct gctgtttatc gaactgggtg ggcagaacct gcttatcact aacccgcgtg
     4861 atattccaga gttggtaaaa gagttagcga aatatccgtt taccgctatc actggcgtta
     4921 acaccttgtt caatgcgttg ctgaacaata aagagttcca gcagctggat ttctccagtc
     4981 tgcatctttc cgcaggcggt gggatgcctg ttcagcaagt ggtggcagaa cgctgggtga
     5041 aactgaccgg acagtatctg ctggaaggtt atggcctgac cgagtgcgcg ccgctggtga
     5101 gcgttaaccc gtatgatatt gattatcata gcggtagcat cggtttgccg gtgccatcga
     5161 cggaagccaa actggtggat gacgacgaca acgaagtacc accgggtcaa ccgggtgagc
     5221 tttgtgtcaa aggaccgcag gtgatgctgg gttactggca gcgtccggat gctacagatg
     5281 agatcatcaa aaatggctgg ttacacaccg gcgacatcgc ggtgatggat gaagaaggat
     5341 tcctgcgcat tgtcgatcgt aaaaaagaca tgattttggt ttccggtttt aacgtctatc
     5401 ccaacgagat tgaagatgtc gtcatgcagc atcctggcgt acaggaagtc gcggctgttg
     5461 gcgtaccttc cggctccagt ggtgaagcgg tgaaaatctt cgtagtgaaa aaagatccat
     5521 cgcttaccga agagtcactg gtgacttttt gccgccgtca gctcacggga tacaaagtac
     5581 cgaagctggt ggagtttcgt gatgagttac cgaaatctaa cgtcggaaaa attttgcgac
     5641 gagaattacg tgacgaagcg cgcggcaaag tggacaataa agcctgagcg ttaagtcagt
     5701 cgtcagacgc cggttaatcc ggcgtttttt ttgacgccca ctaaagagaa aacaatttga
     5761 attaccaaat gattaccacg gacgatgcgc tggcttcttt gtgtgaagcc gtccgtgtct
     5821 ttccggcgat agccctggat actgaatttg ttcgtacgcg cacttattac ccgcagctgg
     5881 ggttgattca acttttcgat ggcgagcatc tggcgctaat tgatcctctc gggatcaccg
     5941 actggtcacc gctgaaagcg atcctgcgcg atccgtccat cacaaaattt ctccatgcag
     6001 gcagtgaaga tctggaagtg ttcctcaatg tctttggcga attaccacaa cccttgattg
     6061 acacgcaaat ccttgctgcc ttctgcggac gcccgatgtc atggggtttc gcttccatgg
     6121 tggaagagta ttccggcgtt acgctggaca agagtgaatc gcgcaccgac tggctggcca
     6181 gaccgctgac cgaacgtcag tgtgaatacg cagcggcgga tgtctggtat ctgttaccga
     6241 tcaccgccaa gcttatggta gaaacggagg cctccggctg gctacctgcg gcgctggatg
     6301 aatgccgcct gatgcaaatg cgtcgtcagg aagtcgttgc gccggaagat gcctggcgtg
     6361 atatcaccaa tgcctggcag ttacgcacac gccaactggc ctgtctgcaa ctgttagccg
     6421 actggcgact gcgcaaggcg cgagagcgtg atctggcggt gaactttgtc gtgcgtgaag
     6481 agcatttgtg gtcggtcgcg cgttatatgc cgggaagttt aggcgaactg gacagcctgg
     6541 gtttatccgg tagcgaaatc cgctttcacg gtaaaacgct gctggcgctg gtggaaaaag
     6601 cgcagacatt gccggaagat gccttaccgc agccgatgct taacctgatg gacatgcctg
     6661 gttatcgtaa agcgtttaaa gcgattaagt cgctgattac tgacgtgagc gaaacgcata
     6721 agatcagcgc cgaattgctg gcatcgcgtc ggcaaatcaa ccaactgctg aactggcact
     6781 ggaaactgaa accgcagaac aatttgccgg agctgatttc cggctggcgt ggtgagctga
     6841 tggcggaagc attacacaat ttattgcagg aatatccgca gtaaaatctt ccgaagccgg
     6901 actgggcgcg ctcagcgcca catccggctt tggcaaacta caaatccaac accaggcgtt
     6961 tacccttcgc acgcgaacaa cagatcaaca tactttgctg gctggcacgc tcttcatcgc
     7021 taaaatactg atcccgatgg tcagcttcac cttccagtat tgccgtttcg caggttccgc
     7081 ataccccttc acgacataaa cattccactt tcgcggcttt attattttca ataacctgca
     7141 aaatagtcat ctcttccggc accacaaact cttttccgga acgggcaagc accagggtaa
     7201 atgcatcgcc ggttttgtct tcgatagcaa attgctcaaa gtgcagtgta tcggcggcga
     7261 tgtccagacg cgcagcttca cttcttaccg cttcaattag cgcctcgggg ccacaggtat
     7321 aaacgtgtgt gccaggttcg atatccgcca ataatcgcgc taattccagt cgtgttccgg
     7381 ttgatgaatg atgcaaatgg actttctcag cctgcggatg ctggactagc tcagcacgaa
     7441 atgcgcaact ttctggattt cgcgagcagt aatgtagctg ccagtcgatg tcgctgtgtt
     7501 gcaactctgc catgtgcgac aggaaagggg tgataccaat accgcccgcg ataaacagat
     7561 gctttctggc tgagggaatt agcgcaaaca ggttattagg cgttgagatc gttaaccgat
     7621 cgcccacttt tacctgctga tgcaaaaagc gggaaccgcc gcgcgagttt tcttccagtc
     7681 gaacggcaat ctgataacta gaggtgttat gcggtgaact cagtagtgaa tacgcattgc
     7741 tgtactggtt atcaccatcg ctcatctgca ctaggatgtg acttcctccg gtgaacgcag
     7801 gcagcggttt gccatcggtt gccaccagcg tgaagcgttt cacctgttcg gtaaggggtt
     7861 caacctggct cacctgtact tcaaacattt gatagtctga cattgctgta ctccatcaaa
     7921 aatcagccca gcggcggaaa ataccgccgc cgatgtcatt agtccttaaa cacctgcgcc
     7981 agcagattat ggaaatgggc gataccatgt tcggaaatgc cactaccgct actgtcggcc
     8041 atgatgcgcc cctgaccacg atagccacgc gatttcagcc ctttctgtac gctttcaacc
     8101 agacgtaaat cttccggatg gaacacatcg cgataccact caatcagcga tttttgctcg
     8161 tcggttaact cttcattggt gaagtaaata tcgtagtttt gcagggtagt ttcagaatcc
     8221 accgggaatt cataaatgac cgtcatcatc cctttgatcg gggtgacgtt cagcatcgtg
     8281 cacggccaca gccagaaacc gtggaatgcc gcatccgtac cctcttcaaa tttaaacgac
     8341 tgttcggacg gtttggcgaa accgtattgc agcgtccagt taccgtgcat ggtgtgccag
     8401 taacgatcaa cctgtacgga gtcggagaaa cctagatgcg ccggaccaca gtgatagcac
     8461 tcgagatagt tatcgacaat gttcttccag ttggcaggcg tgcgggtggt aaagcgggcc
     8521 gctagtttca gattgtggac ttccgggcag gcttccagca ctttcgcgcc cagaccggtg
     8581 tattgattca cttgaagtac gaaaaaaaac cgggaggaca ttggattatt cgggttctga
     8641 tgggattaga tttggtgggg cttgcaagcc tgtagcgcaa attttagtcg ttaatcaatg
     8701 aaacgcgaaa gatagtaaaa aattgctttt gtttcattga aaatacgaaa aacaaaaaca
     8761 ctgcaaatca tttcaataac agcttcaaaa aacgttcaaa accgataaca accaggctgt
     8821 aaccaaatga ctcatatcac aaatcagctc atgccgttta ggtatgttac atgtgtgatt
     8881 atgtgaggtg aagtatgttt tagctggttc atggttgtta tacggctttt tttacatctt
     8941 gtggttcttg tgaaggagct acaagacctt cctgttcata aataccatac tttgacaaaa
     9001 tctttttacg agtatctgtt ggtaatgatt caagcatttt ggctgtttgt aaggtttgct
     9061 gcgaaatacc ttgcaaatca gcctggatgg aatcatcgga agaaaaaatc tcaggcataa
     9121 gagcttcaaa tcctttcttt ctgaacaact caatcgcaga atgcagttcc ttgtcctcta
     9181 acaattcgac aattgcgatc agtttagttt tcaatgtgct gcggtcaggt tgagaatttt
     9241 tattctcttg attattttta tgctcaacca ttggcatgct ctgactttgg ataaaccact
     9301 ctattggacg ccctgttgcg taggctattt gtgctaatcg atctaaagac gggtatgtct
     9361 tgccagacag gtagtttctg ataaccccat cagaaagtcc gcacttagct gcaaacgccc
     9421 ggcttgattc tccattcatt gcctcttcaa ggcgttcctt gaaacgcatt atttccgggg
     9481 gcataaaaat gcgctctgca tttttgcttt ctttttcacc tgtcatgccc aatctctctg
     9541 ttatataaag aattttacag aattctttga tttgcactgg ttaaaataaa aatgcgcata
     9601 gctcttgaat tgagcatttt tctgcgatat cgtttttccc gatagcggta gccggtatca
     9661 gttaccgaaa acgttaagag agtaaggatc gcagaatgtc agcaaaaaac caagactggc
     9721 acgcagagca gataaaggct gctgttcgta tgaaggggaa gacactatct caattatcca
     9781 gaga
//
LOCUS       DAECOD010000128         9726 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.128, whole genome shotgun sequence.
ACCESSION   DAECOD010000128 DAECOD010000000
VERSION     DAECOD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.128"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..287
                     /locus_tag="KKO15_003438"
     CDS             <1..287
                     /locus_tag="KKO15_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6362101.1"
                     /translation="GWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSE
                     CFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            384..536
                     /gene="hokE"
                     /locus_tag="KKO15_003439"
     CDS             384..536
                     /gene="hokE"
                     /locus_tag="KKO15_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD6362102.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            887..1039
                     /gene="hokE"
                     /locus_tag="KKO15_003440"
     CDS             887..1039
                     /gene="hokE"
                     /locus_tag="KKO15_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD6362103.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1161..1781)
                     /gene="entD"
                     /locus_tag="KKO15_003441"
     CDS             complement(1161..1781)
                     /gene="entD"
                     /locus_tag="KKO15_003441"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBD6362104.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1956..4196)
                     /gene="fepA"
                     /locus_tag="KKO15_003442"
     CDS             complement(1956..4196)
                     /gene="fepA"
                     /locus_tag="KKO15_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBD6362105.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKNENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4439..5641
                     /gene="fes"
                     /locus_tag="KKO15_003443"
     CDS             4439..5641
                     /gene="fes"
                     /locus_tag="KKO15_003443"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBD6362106.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5644..5862
                     /gene="ybdZ"
                     /locus_tag="KKO15_003444"
     CDS             5644..5862
                     /gene="ybdZ"
                     /locus_tag="KKO15_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBD6362107.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5859..9704
                     /gene="entF"
                     /locus_tag="KKO15_003445"
     CDS             5859..9704
                     /gene="entF"
                     /locus_tag="KKO15_003445"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBD6362108.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2155 a         2523 c         2669 g         2379 t
ORIGIN      
        1 agggatggca gtatcgtatg agaagatatc aaaatatcct taaagaacat ggtattaaac
       61 aaagcatgtc cagaaaaggc aattgtctgg ataatgctgt ggtggagtgt ttctttggaa
      121 ccttaaagtc ggagtgtttt tatcttgatg agttcagtaa tataagcgaa ctgaaggatg
      181 ctgttacgga atatattgaa tactataaca gcagaagaat tagcctgaaa ttaaaaggtc
      241 tgactccaat tgaatatcgg aatcagacct atatgcctcg tgtataactg tccaactttt
      301 tggggtcagt acaccctggg gctgccactc caaaccagac aatttggatg gtagtccctt
      361 cttcgcatgg aggcaatata aacatgctga cgaaatatgc ccttgtggca gtcatagtgc
      421 tgtgtttgac ggtactggga tttacgcttc tggccggaga ctcgctgtgt gagtttacgg
      481 tgaaggaacg taatattgag ttcagggctg ttctcgctta cgaaccgaag aagtagccgt
      541 tgtgcgggga gtaatccccg catatccggt tgtcaggtca ggatggtaag gcacctgctt
      601 tacactttcg cccgcggtca gtgatggctg cgggcgattt cgttttaaga gcaatgatga
      661 gttttgccga cagaagtccg aaacaggact cactgtataa ataaacagct attttgctaa
      721 ggaagggtaa gataacggcg ggtgcctgaa gctttccggt ttcaggttaa ctctgaggtt
      781 tggaaagatg aagccccaag agatatttct atcaatcctg gggctgccac tccgaacctc
      841 aacaactcgg atggtagtcc cttcttcgca tggaggcaat ataaacatgc tgacgaaata
      901 tgcccttgtg gcagtcatag tgctgtgttt gacggtgctg ggatttacgc ttctggtcgg
      961 agactcgctg tgtgagttca ccgtaaagga acgtaatatt gagtttaagg ctgttcttgc
     1021 ttacgaaccg aagaagtagc cgttgtgcgg ggagtaatcc ccgcatatcc ggttgtcagg
     1081 tcagagtggt aaggcacctg ctttacactt tcgcccgcag tcagtgatgg ctgcgggcga
     1141 atcgtaccag atgttgtcaa ttaatcgtgt tggcacagag ttatgactat tttttctttt
     1201 atctgccagt gcacagcaaa catctcattc tcacgatgaa tgatgacctg ctgtttattc
     1261 cagctaatta tctgatagtc cagaaaacct gcatcagttt ggatctcact tgccttaaat
     1321 gcgctctctt tggcggaaaa tgcaagtgtc agcgccagag aaaaggctaa accgcagtct
     1381 gcgagtcgct cgtgttctgc tggtgtaata atgttgtctg tcaattcggt cgctgtttgc
     1441 gcagaaaaaa tttcttcgat atcaacgcca atcggttgac gggaaaccac ggccaatgcc
     1501 gtagccccgc agtggctaat actgccgtat acctccgcag gccacaccgg ttggcgtagc
     1561 tcgccgattg cgggcacaca tttatagcca tattcccgca aggcataaac agcagcgatc
     1621 cgtccggcta aatgctctgt tttacgttta cgtccagcgt gttgcagttg tgcgtagtgc
     1681 ggcagccaga gtaaatcctg ctcacaaaaa ttcgccggat cgaactcaac aaaatgcagc
     1741 gtatgtccgg caaaggggag ggaggtatgc gtagttttca tatcgaccat attcgagact
     1801 gatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg atcctgcaat
     1861 atattgaatt ggcaagatct ttgtaggccg gataaggcgt tcacgccgca tccggcgtga
     1921 acgacgcgca ctttgtcaac aatctgacgt tagcatcaga agtgagtatt aatgctcata
     1981 taccacgtac gtcccggctc gttataggta tacgcgccgg caccggcgat atagttggct
     2041 cctgccagat cgcccgtggt ctgggcatta cctgcacgcc acaaacgttt gtcgaacagg
     2101 ttgtccacgc cgccggtcag actgacattc ttcgtcacat cccaggtcgc gctcagaccc
     2161 acaatgctgt aaggactgat ttctttggtt tccgggccaa ccgctggctg acctttatag
     2221 ttgtacttct tcggctgctg cttgccgtac caggtgaagg tcgtttgcat cgacaaatct
     2281 tcccgtgcct gccagctcag cgttgagttc aacgtgtact ccgggatgat cgacaaacgg
     2341 tcgcccgtgg ttttgttttc attcttcagc atataagtga tgttattggt ccacatcacc
     2401 gtttcgctaa ctggtacgtt taacgatcct tccagacctt caaccactgc tttcggcacg
     2461 ttatcccact gatagagatc agtgccgact gcgttttgcc ctacagccac atagcccgct
     2521 tcaatcttat tgcgataatc gttacggaac caggtgacgc ccgccagcca gccgtcgcgt
     2581 ttgaactcca gaccaatctc tttgttgatg ctggtttcta ctttcaggtc atcgttacct
     2641 tgcagatagc agccgcccgc gctggcatag cagccctgac ctttactgta gagaatgtag
     2701 ttcgggttag tctggtatag gctcggcgct ttataggcgc gggcaatgcc tattttcagc
     2761 gtgaagtcat cgcctaaacc ttgcgatatg ttcagcgccg ggctccagtt attgccgaca
     2821 atactgtgat gatcgaaacg cagccccggc gttacgatgg tgctgtcagt cagctccatg
     2881 ttgttttcgg caaacagcga gaaaatttct gcttttgaat acgggctacg gtcggtggca
     2941 ctcacgccat caatagcgcc accggtattc gttccggtca gtgcttgggt gttggaactt
     3001 aagtccttca tccgttgctg attccactcc gtacccagcg tcagcgtctg gttaacgagg
     3061 aaatcaatcg gcaggttaac ttcgctgtgc agcatcacgt catcgagatc gatatcgacg
     3121 aaatcctgtg tcgctttttc gttaaatttc ccttcggtac cgcccgccag accttccgga
     3181 atacgcgagt tacgggtgtg ttcatactgc acccagttgc tggtggtcac gccgttatcc
     3241 cagccaccgt tccaggtcag cgcgtagttc tggcgataca ggcggttggt ttcatcgcca
     3301 tatttcgagc gagtgtaagc gtcagagttg gtgttctggg tatcgcccgc atacaggtta
     3361 ccctggcggc tgtaacccgc ttccagttcc agcgattgca atggtgcgaa gtcccagcgc
     3421 accacgccgt taatatcttt attgatcacc ccttcgcgcc cggctggtaa cgtcgtggca
     3481 tacgttccgg cacgcgcgga ctgatggccc tggttgatat cccacgcgtc agcctgagtt
     3541 ttgtcgaggt tgccatacaa gcggaagctg aattcgtcgc ccagcggacc ggtcaggcta
     3601 aagttagtgc gtttggtggc accttcctct ttatgttctg gcgcattgaa atatgcgtcc
     3661 caggagccgt gccactcgcc gctgcctttt ttggtaatga tgttaaccac gccgcccgcc
     3721 gcgccgttgc cataacgcgc agctgccgga ccacgcagaa cttcaatacg ttcaatcatt
     3781 tcaggtggca cccaggaggt atcaccacgg gtatcgcgct cgccacgcca gccctgacgc
     3841 accgagttac ggctgcttac cggcttgccg tcaatcaaaa tcagcgtgtt ttccggaccc
     3901 ataccgcgaa tatcaatctg tcggttattc ccacgctgac cactggtgga gttaccggtc
     3961 aggttaacgc ctggcatggt acggatgatc tccgacacat cgcgggcaac cgggtttttg
     4021 cggatttcat ctgcggtgat ggtcgaaacg ccaggagcct gcaagttctg ctcggcggcg
     4081 gtaacgacaa tggtatcgtc atgtgaaaca ggtgtatcgg tcggctcttg tgcctgcgct
     4141 accccataaa tccccagatt gaccaacaag gccagggaat gaatcttctt gttcattgtt
     4201 ttattcctgc atttttgcca cgaattgcaa ctgtcgggca tggtcgtcat caacacgacg
     4261 catcccgtta ccgcgaaaac ctttgatcct gaaagacacg cagtgcagtt ggttaattaa
     4321 tgtccgcgct tcccacggcg cgccattacg ctattgcaaa tgcaaatagt tattaataac
     4381 attatcaata tatttctgca atcaatgaaa aattgcacag taaacatggg gttatggtgt
     4441 gacggcgtta aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc cggaatggca
     4501 gcgtctgaat gacgaaatgt ttgaggtcac tttctggtgg cgtgatcccc aaggttctga
     4561 agaatactcg acgataaagc gcgtatgggt ctacatcact ggtgtgaccg atcatcatca
     4621 gaacagccag ccccagtcga tgcagcgaat tgcaggtact gatgtctggc agtggacgac
     4681 acaactcaat gccaactggc gcggcagcta ctgctttatt cccaccgaac gcgatgacat
     4741 tttttctgca ccatcccccg atcgcctcga attacgtgaa ggctggcgaa aactattacc
     4801 ccaggcaata gccgatccgc tgaatccaca aagctggaaa ggcgggctag ggcacgctgt
     4861 ttctgcactc gaaatgccgc aagcgcctct gcaaccggga tgggattgtc cgcaagcgcc
     4921 agaaatacca gccaaagaaa ttatctggaa aagtgaacgg ttgaaaaatt cacgtcgtgt
     4981 atggattttt accaccggcg atgtaacagc agaagaacgc ccgctggcag ttttgctcga
     5041 tggcgaattt tgggcgcaaa gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg
     5101 tcagcaactt cctcccgccg tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc
     5161 ccacgaactg ccgtgtaatg cggatttctg gctggcagta cagcaagagt tattacccct
     5221 ggtgaaagtt attgcccctt ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag
     5281 ttttggtggg ctgtccgcgc tgtatgccgg actgcactgg cctgaacgct ttggctgtgt
     5341 attaagccag tcaggatcgt actggtggcc gcatcggggc gggcagcaag agggcgtgtt
     5401 acttgaaaag ctaaaagctg gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc
     5461 gggtattcgc gagccgatga tcatgcgggc caatcaggcg ctgtatgcgc aattacaccc
     5521 tataaaagaa tccattttct ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg
     5581 cggtggcttg atgcaggggt taatcgacct ctggcaacca cttttccatg acaggagttg
     5641 aatatggcat tcagtaatcc cttcgatgat ccacagggag cgttttacat tttgcgcaat
     5701 gcgcaggggc aattcagtct gtggccgcaa caatgcgcct taccggcagg ctgggacatt
     5761 gtgtgtcagc cgcagtcaca agagtcctgc cagcagtggc ttgaagccca ctggcgtact
     5821 ctgacaccgg cgaatttcac ccagttgcag gaggcacaat gagccagcat ttacctttgg
     5881 tcgccgcaca gcccggcatc tggatggcag aaaaactgtc ggatttaccc tccgcctgga
     5941 gcgtggcgca ttacgttgag ttaaccggag aggttgatgc gccattactg gcccgcgcgg
     6001 tggttgccgg attagcgcaa gcagatactt tgcggatgcg ttttacggaa gataacggcg
     6061 aagtctggca atgggtcgat gatgcgctga tattcgaact gccagaaatt atcgacctgc
     6121 gaaccaatat tgatccgcac ggtactgcgc aggcattaat gctggcggat ttgcaacaag
     6181 atttgcgcgt cgatagcggt aaaccactgg tctttcatca actgatacag gtggcagata
     6241 accgctggta ctggtatcag cgttatcacc atttactggt cgatggcttc agttttccgg
     6301 ccattacccg ccagatcgcc aacatttact gcgcattgct gcgtggcgaa ccaacgcctg
     6361 cttcgccgtt tacgcctttc gctgatgtag tggaagagta ccagcaatac cgcgaaagcg
     6421 aagcctggca gcgtgatgcg gcattctggg tggaacagcg tcgccaactg ccaccgcccg
     6481 cgtcactttc tccggcacct ttagcggggc gcagcgcctc ggcagatatt ttgcgtctga
     6541 aaatggaatt taccgacggg gaattccgcc agctggctac gcaactttca ggtgtgcagc
     6601 gtaccgattt agcccttgcg ctgacggctt tgtggctggg gcgattgtgc aatcgcatgg
     6661 actacgccgc cggatttatc tttatgcgtc gactgggctc ggcggcgctg acggctaccg
     6721 gacccgtgct caacgttttg ccgctgggta ttcatattgc ggcgcaagaa acgctgccgg
     6781 aactggcaac ccgactggca acccgactgg cagcacaact gaaaaaaatg cgtcgtcatc
     6841 aacgttacga tgccgaacaa attgtccgtg acagcgggcg agcggcaggt gatgaaccgc
     6901 tgtttggtcc ggtactcaat attaaggtat ttgattacca actggatatt cctggtgttc
     6961 aggcgcaaac ccataccctg gcaaccggtc cggttaatga ccttgaactg gccctgttcc
     7021 cggatgaaca cggtgatttg agtattgaga tcctcgccaa taaacagcgt tacgatgagc
     7081 caacgttaat ccagcatgct gaacgcctga aaatgctgat tgcccagttc gccgcggatc
     7141 cggcgctgtt gtgcggcgat gtcgatatta tgctgccagg tgagtatgcg cagctggcgc
     7201 agatcaacgc cactcaggtt gagattccag aaaccacgct gagcgcgctg gtggcagaac
     7261 aagcggcaaa aacaccggat gctccggcgc tggcagatgc gcgttacctg ttcagctatc
     7321 gggaaatgca cgagcaggtg gtggcactgg cgaatctgct gcgtgagcgc ggcgttaaac
     7381 caggggacag cgtggcggtg gcactaccgc gctcggtctt tttgaccctg gcactccatg
     7441 caatagttga agctggcgcg gcctggctac cgctggatac cggctatccg gacgatcgcc
     7501 tgaaaatgat gctggaagat gcgcgtccgt cgctgttaat taccaccgac gatcaactgc
     7561 cgcgctttag cgatgttccc aatttaacca acctttgcta taacgccccg cttacccccc
     7621 agggcagtgc gccgctgcaa ctttcacaac cgcaccacac ggcttatatc atctttacct
     7681 ctggctccac cggcaggccg aaaggggtaa tggtcgggca gacggctatc gtcaaccgcc
     7741 tgttgtggat gcaaaatcat tatccgctca caggcgaaga tgtcgttgcc caaaaaacgc
     7801 cgtgcagttt tgatgtctcg gtgtgggagt ttttctggcc gtttattgcc ggggctaaac
     7861 tggtgatggc tgaaccggaa gcgcaccgcg acccgctcgc tatgcagcaa ttctttgccg
     7921 aatatggcgt aacgaccacg cactttgtgc cgtcgatgct ggcagcattt gttgcctcgc
     7981 tgactccgca aaccgctcgc cagagttgcg tgacgttgaa acaggttttc tgtagtggtg
     8041 aggccttacc ggctgattta tgccgcgaat ggcaacagtt aaccggcgcg ccgttgcata
     8101 acctgtatgg cccgacggaa gcggcggtag atgtcagctg gtatccggct tttggcgagg
     8161 aactggcaca ggtgcgcggc agcagtgtgc cgattggtta tccggtatgg aatacgggtc
     8221 tgcgtattct tgatgcgatg atgcatccgg tgccgccggg tgtggcgggt gatctctatc
     8281 tcaccggtat tcaactggcg caggggtatc ttggacgacc cgatctgacc gccagccgct
     8341 ttattgccga tccttttgct cctggtgaac ggatgtatcg taccggagac gttgcccgct
     8401 ggctggataa cggcgcggtg gagtacctcg ggcgcagtga cgatcagcta aaaattcgcg
     8461 ggcagcgtat cgaactgggc gaaatcgatc gcgtgatgca ggcgctgccg gatgtcaaac
     8521 aagccgttac ccacgcctgt gtgattaacc aggcggcagc cactggtggt gatgcgcgtc
     8581 agttggtggg ctatctggta tcgcaatcgg gcctgccgtt ggataccagc gcattgcagg
     8641 cgcagcttcg cgaaacattg ccgccgcata tggtgccagt cgttctgctg caactgccgc
     8701 agttgccact tagcgccaac ggcaagctgg atcgcaaagc cttaccgttg cctgaactga
     8761 aggcacaagc gccagggcgt gcgccgaaag cgggcagtga aacgattatc gccgcggcat
     8821 tcgcgtcgtt gctgggttgt gacgtgcagg atgccgatgc tgatttcttt gcgctgggcg
     8881 gtcattcgct actggcaatg aaactggcag cgcagttaag tcgacagttt gctcgtcagg
     8941 tgacgccggg gcaggtgatg gtcgcgtcaa ccgtcgccaa actggcaacg attattgatg
     9001 gtgaagagga cagctcccgg cgcatgggat tcgaaaccat tctgccgttg cgtgaaggta
     9061 atggcccgac gctgttttgt ttccatccgg cgtccggttt tgcctggcag tttagcgtgc
     9121 tctcgcgtta tctcgatcca caatggtcga ttattggcat tcagtcgccg cgccctcatg
     9181 gacccatgca gacagcgaca aacctggatg aagtctgcga agcgcatctg gcaacgctac
     9241 ttgagcaaca accgcacggc attgcggcgc ggctgcgtgc ccgtggcgaa caggtggcat
     9301 ttcttggttt gctggatacc tggccgccag agacgcaaaa ctggcaggaa aaagaggcta
     9361 atggtctgga cccggaagtg ctggcggaga ttaaccgcga acgcgaggcc ttcctggcgg
     9421 cacagcaggg aagtacttca acggagttgt ttaccaccat tgaaggcaac tacgctgatg
     9481 ctgtgcgcct gctgacgact gctcatagcg taccgtttga tggtaaagcg acgctgtttg
     9541 ttgctgaacg cacgcttcag gaaggtatga gccccgaacg cgcctggtcg ccgtggattg
     9601 cggagctgga tatctatcgt caggattgtg cgcatgtgga tattatctct ccaggggcgt
     9661 ttgtaaaaat tgggccgatt attcgcgcaa cgctaaacag gtaaattaat attatctata
     9721 aaccca
//
LOCUS       DAECOD010000129         9683 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.129, whole genome shotgun sequence.
ACCESSION   DAECOD010000129 DAECOD010000000
VERSION     DAECOD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.129"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..85
                     /locus_tag="KKO15_003446"
     CDS             <1..85
                     /locus_tag="KKO15_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6362109.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            805..1233
                     /gene="uspC"
                     /locus_tag="KKO15_003447"
     CDS             805..1233
                     /gene="uspC"
                     /locus_tag="KKO15_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBD6362110.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1240..2654)
                     /gene="otsA"
                     /locus_tag="KKO15_003448"
                     /pseudo
     CDS             complement(1240..2654)
                     /gene="otsA"
                     /locus_tag="KKO15_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2629..3429)
                     /gene="otsB"
                     /locus_tag="KKO15_003449"
     CDS             complement(2629..3429)
                     /gene="otsB"
                     /locus_tag="KKO15_003449"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBD6362111.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3596..4582)
                     /gene="araH"
                     /locus_tag="KKO15_003450"
     CDS             complement(3596..4582)
                     /gene="araH"
                     /locus_tag="KKO15_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD6362112.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4597..6111)
                     /gene="araG"
                     /locus_tag="KKO15_003451"
     CDS             complement(4597..6111)
                     /gene="araG"
                     /locus_tag="KKO15_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBD6362113.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6181..7170)
                     /gene="araF"
                     /locus_tag="KKO15_003452"
                     /pseudo
     CDS             complement(6181..7170)
                     /gene="araF"
                     /locus_tag="KKO15_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7967..8470
                     /locus_tag="KKO15_003453"
     CDS             7967..8470
                     /locus_tag="KKO15_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBD6362114.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8550..8801)
                     /locus_tag="KKO15_003454"
     CDS             complement(8550..8801)
                     /locus_tag="KKO15_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBD6362115.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8916..9002)
                     /gene="azuC"
                     /locus_tag="KKO15_003455"
     CDS             complement(8916..9002)
                     /gene="azuC"
                     /locus_tag="KKO15_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBD6362116.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVLPGNMFR"
     gene            9265..9588
                     /locus_tag="KKO15_003456"
     CDS             9265..9588
                     /locus_tag="KKO15_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBD6362117.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2573 a         2396 c         2161 g         2553 t
ORIGIN      
        1 gcggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
       61 tctgaacata aaacactatc agtaagttgg agtcattacc aaaataatta gcattagaat
      121 agttgcgata agctgcaata agcagagcca cctttttggt ttaataagtc cttacaaata
      181 gaaggggggc tttacactta tctaagattt ttcctaaatc gacgcaactg tactcgtcac
      241 tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg cagatcacac
      301 aaaacactca attacttaac ataaatgtat aagtcatact tttgttttgg gtgtatttca
      361 atctgttaaa aagtttttcg ctacgctagc aagcaaaaat gaaacaggaa taatcgaaat
      421 gggatgttgc gcacggttaa aataactcac cgtaaataat catctgctat aaataatcac
      481 tttcatgcaa taccagataa gctattttta aacagacact taccgcacaa caaactaatt
      541 aaatacaaag aaaggcaaca atcaacttaa tgttaataaa atgtttttca tatattaacc
      601 acttgtttga tgcggggaat cagtacatta ttttcttata attacagttg aaatcatatg
      661 atcaccgaga gtgattgatt aattgtcttt actaataatc agactaatat ttacctgttt
      721 gactgagttg ggattgcgtc gtttctccat taggagtaaa gctttaatgt cacctgaagt
      781 tcacagcaga ataaggaaca ggttattagc tatagcaata ttcttgtcgc tgttgcggtt
      841 acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc agtaaaggga
      901 cacatcagtt taattactct cgcttccaac ccggaaatgt acaatcaatt agccgcgccg
      961 atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct tgataagtta
     1021 attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga gttaagcgaa
     1081 catattctgg aagtatgtca caaacatcat ttcaatttgg tgatttgcgg taatcacaat
     1141 cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc aagtgaggtc
     1201 gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct ttggaaaggt
     1261 agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct ttaggtcgct
     1321 aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca gcatttctgc
     1381 atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg cagctgcaac
     1441 ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg ccgctcccgc
     1501 aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa catactcttt
     1561 tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag agtagcggaa
     1621 tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg gcgtccagcc
     1681 taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat gacgaatatc
     1741 ctggtaggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac gaattttacc
     1801 atgatgctgc ggatattttt ccagcaatgc ctcataggcg agaaaacgct ccggcaaacc
     1861 tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca attccgcttt
     1921 aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt ctttcggttc
     1981 aatgccgatc gggtagactt ctgttcgaaa tgcttttccc caggctgtat ggctttttgc
     2041 gctacgtgtc gtgacgcggg tcaggttaga aaggcaatcc aggaacgcca gacgatcgtt
     2101 ttctgtctgt aaacccagca aatcataatc acaaagctgt tcaagcaagg tgtcatatgt
     2161 cggcagcgcg ttgaaaattt ccggtgtcgg gaaaggaata tgcaagaaaa aaccaatgcg
     2221 attattcacc ccacgtttgc gtaattcatg cgcaaagggc aacaggtgat aatcgtggat
     2281 ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca acgcatttac
     2341 gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc gataatgaaa
     2401 agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt cctgttcgct
     2461 gaggttaaaa gaggcccacg taatgttacc ttttttagcg gctgatcctc attccctgtt
     2521 tcaccactcc agccaaacca cagtccgcct gcggctttca gtgcccccag tatgccaacg
     2581 gcaaggccac cggcactggc ggcgtgctcg tctggtggtg caatccggtt agatactacg
     2641 actaaacgac tcatagtcat cactcctgtt attttctctt ttttgttgta atgcggcggt
     2701 tatcatttca agccagctcc agacatccgg cacacccgcc agtcgccatg atgcctgagt
     2761 tgcacctgtg ccaattttta ctgacattcc gcccagtcgg ttaacgactg cgaagccaga
     2821 ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc ccgataaaag gagcttcctg
     2881 cataaaggct gcaatcgctt cacctttact ggtacctctc ggtttgatct cgacaacaca
     2941 ctttccctgc tgtaacgcca tttgtggcca gatctgagta atacgttgcg ctaatgtcat
     3001 taatgcgtct tcatgctgcg gagcctgacg ataatgcagc gcaaaagcca tccctttcgc
     3061 ctccagctcc gcgccgggat actgagcgat gaccgtatgc agttgcacgc taatatcacg
     3121 cgcaatcgca tccggcagat gaacgatatg tgttttacca ttgatgtcac ggcgctccgc
     3181 cccatgcacg cccgctaacg ggaagcgata aggttttgcc agtgcgtcaa gctccaccat
     3241 tgagcgccct gatatcaatg ccaatgcacc atcacttgcg gttgccagta gctgtagtcc
     3301 ttgcagaata ttgtcaggca cgacgacctg atcgggatgc ggtttgattt ccgccagcgt
     3361 tccatcaaga tcaaaaaacc aggcatattt cgcggatagt tcaggggttt cggttaacgg
     3421 ttctgtcacc cggctctcct ccttctcttt tcttattgtg ttgcgcagac cgcgctttac
     3481 agacatgtaa gtatagacag tgtgacggag gtcgccattt gcaacgaaga acagccaacc
     3541 gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg ttgtggaaaa aagcatcaga
     3601 cagtgcgctt cgctttttgc ttgtaacggt cgaagatcac cgctgccagc aggattaagc
     3661 cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag gttcatggcg ttttccacgg
     3721 tgcctaaaat taagataccc gccaccacat atgagatttt tccgatgcca cctttcagag
     3781 aaacgccacc taaaacgcag gcggagataa caataagctc ataaccaatc gacgtcattg
     3841 gctgcccact agtcatacgt gaagccagaa taattccggc tatcgcagat accaggccag
     3901 agagaacaaa gataataatt ttggtgcgaa caaccggtac acccgccaga cgcgcagcct
     3961 cttcgttccc gccaatcgcc agggtattac gaccaaaggt ggttttattc agcagcaaac
     4021 caaagataat cagacacgcg acggtgagcc agattggcgc aggcagaccg aaccagttag
     4081 cgtaaccaag ggcaaagaag ctttcatctt cgataccgac cgctttaccg tctgaaatga
     4141 tgtacgccag acctcgaaca atctgcatcg ttgccagtgt cgtgatcaga gcatttattt
     4201 tcagtttggc gataacaaag ccattgacca ggccacagag aacgcccagc aacaaacccg
     4261 ctgccacgcc aatccacagg ctttcagtca ggttgataac caccgccgtg gtgacacccg
     4321 cacaggcaat tacggaggcg acagaaaggt caaagtcacc ggaagcaagg cagaacaaca
     4381 tgccacacgc caccatcccc gacatggaaa ttgccaggcc caaccctttc atattaatga
     4441 aggtggcaaa atttgggaca aaaatggcac aggcgataaa gagcaccgca aacaccacca
     4501 gcatgccgta ctgatcccag atacgcccga agctgaatga cgacttaggt gcgccagacc
     4561 ccgatgtaga aacagaagac atcatactct ccttactcag gcaactgcct ggctgacttt
     4621 aggcatcgca aggctcagtg cctgacgctc atctgcctgc tcgtgtaaca attcaccggc
     4681 gatttcgcct tcccgcatca ccacaatccg gtcggcaacg ccgaggactt caggtaagtc
     4741 gctggaggca aacagcaccg ccacgccctg cgccgccagc gcataaatta cgttatatat
     4801 ttcgtgctta gcgccaacat caatgccgcg cgtaggttca tccagcaaaa tgaccttcat
     4861 ctcttccgat aaccagcggc ccagaatggc tttttgctga tttccgcctg agagattcat
     4921 gatcagttgc tccgcgcccg gcgttttgat gttgagcgaa cgaatgtgct gatcggcatt
     4981 gttttcttcc caactgttgt tgattacaca accgccgagc acatgtttgc gtctggcact
     5041 gatgttgata ttgtcgcgaa cggagtgcac gggaataatg ccttccgctt tgcgagcttc
     5101 cgggcagagc atcatgcctg cggcaatggc gtggctcggt ttacgaatat cgatcggctg
     5161 ttggtcgata taaacctgac cggcggtgat ttgcgtcccg ccaaacaagc ctttcattaa
     5221 ttcgctacgc cccgccccta ccagaccaaa cagcccaaca atttcaccac tgcgaaccgc
     5281 cagacttatt ggcgtacgca cgcctagtgc tttcacagca tcaagacgca ggcgctcctc
     5341 gccataacta cgaggttgcc agccgtagat atcgccaatg tcgcgcccga ccatcgcctg
     5401 caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag gttttgacat aacgtccatc
     5461 tttaaagaca gtgatggcat cgctgagggc aaatatttct tccatacggt gagaaacgta
     5521 taagatcacc cgcccctctt ttcgcagttc acgaataacg cggaaaagat tgccgatttc
     5581 gcgggcagaa agggagctgg ttggctcatc aaaggcgata attttagcgt tacgcgccag
     5641 cgctttggcg atttcaacca tctgccactg accaatggcg agatatttca gcggcgtgtc
     5701 cgggtcaata tccataccaa gatgtttaag ttgtaaaccc gcctcataat tcagcaatga
     5761 gcgattcaca atgccgcctt tatgcggcag ctggccgaga tagatgtttt ccgcgacggt
     5821 catttccggc acgagatgca gttcctggta aataatcgcc acacccgcgt taagtgctgc
     5881 ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact acagaacccg tggttggcgc
     5941 atagttgccg ctgaggattt ttaagagagt tgattttcct gcgccatttt cacccatcaa
     6001 cgcatgaacc tgaccggcat agcagtcaaa actaatatcc gtcagcgcct taacgccggg
     6061 aaacgtttta ccgatgccgc gaaatgagag atacggggta gactgttgca taacgtctcc
     6121 gtgaatcact ggtcgttcac accccctctg catcatgcag aggggaattt ttccggcaaa
     6181 ttacttaccg cctaaacctt ttttctccag ttcttcttta aagttgtcac gcgtgatcag
     6241 taccacgtct gtaacttcgg taaattttgg cgtttcaacg tcttttgcta cccagttgta
     6301 aagcatttcg ctggatttat agccatgtac gtccgggctt ggcagcaggg aaccgtagaa
     6361 gccggttgcc tgtgctttag acagttcgct tactgcatcc acaccgttaa taccaatacc
     6421 gatgatatcg gctgctttaa agccctgacc ttcggttgca cgtacgccgc caagtacggt
     6481 gctgtcgttc ataccgacga tcagccaatg tttaacttcc ggatgttgaa ccagcattga
     6541 gttggcagcg tcaaatgccc ccgggatgtc gttagatttg gtaggtacct gataaatttg
     6601 tttttccggg aatcctgcag ctttcagtgc gtccatagaa cccgttgtac ggcggcgggc
     6661 ggtatccagt tcgttggcgg taatcgccat cactgcgctt tctttgacat cccagccacg
     6721 tttctgcatc tctttataca gttcctagcc ctgacgttcg ccaattttag tcgccgccat
     6781 catcaccaga ggaacggtat ccattggctt acctttggcg ttaacaaact ggtcatccac
     6841 tgcaatgact ttcatatcgt agccacgcgc tttcgcgacg atggcagagc cgagtttcgg
     6901 gtccggagta caaataacga aaccttttgc gccactggct gccaggctgt cgatcgcgtt
     6961 caatgttttt tcgccatccg gcacggcaat cttaataacc tcaaacccta aatccttccc
     7021 ggctttatcg gcaaacttcc attcggtctg gaaccacggc tcttccggtt gcttcaccag
     7081 aaaaccgagc ttcaggttct ccgccatagc ggattgtgac ataacggctg ccagaccaat
     7141 ggctgccagg gctttagtaa atttgtgcat ggttctctcc agctttagtg tcgttttgtg
     7201 tagtgcaaaa acgaatgaca ttcgttaaat taatcggaaa acaaagcatt accttttatc
     7261 taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa attaattctc tataggagag
     7321 caatatcaca tcgcagaatt acagtgagaa cgtgcataaa tttagcggga aaagacataa
     7381 ggaaaagcca atttgtcaga caaattgttg aatgcacagc agattaatcc ataagattag
     7441 cctggaaatc cttgttgtct tcggtaccca tgcgggatgt cttcttttta accagtcaat
     7501 aggccgcatt acctggcgtt gagtttttga aatggtgtaa taaccacaac tcaaagatgt
     7561 ggaaaatgcg cgtcattcat ttcgccatta attatcactg tgctcattaa ttaacagaac
     7621 acgtataatg agagccatct cgcaaaaatg aaaaaacgtt ttataaaatc atcacttcat
     7681 catgaattca aattcatcga ttaatatcaa caagatacaa aaagcactat cattaaaatt
     7741 cattgcattt acattgattt caccaatgaa atgtaaaaat atataaactt gatgatttaa
     7801 gcattttctt ataaccgttc agacgttatt cttatttcag atcatcgtca gaattgactc
     7861 cacgatcaca tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt
     7921 tgatttatct tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg
     7981 aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct
     8041 tcacctgagt aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc
     8101 ccaggcacag agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg
     8161 cgctaccccc atcgttaaag ccattgatgt tccaggtgaa aaactgaact ctctggaaga
     8221 actgttccaa aaaacgatgg aagaatacga gcaacgttca agcactctgg cgcagttagc
     8281 cgatgaagcg aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa
     8341 agaacagcag catgatggcc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa
     8401 acttgcgggt atgtgccctg tgcagaccga ccaacatgtt ctgaatgtcg tgtcacacca
     8461 gctgcattga tcatacatcg gcgctaatac attgcgccga tgaagttttt gagaaaccgc
     8521 tgcctcatct gtttgaagca gcggtttttt taatgggatt caccctgtgg ggtaaacttg
     8581 agttcaatca gcgcgatggc tttttggatt gcccgcatgg tgaccgggtc tgcggcagcg
     8641 ggatgatgag taaagtcgat attcttcagc tgactggaca ttttttcacg aacttcaacg
     8701 ggcgcgatta catcgagaac atccagaatt tgtttgataa ccaactggca agctaccaca
     8761 tcagaaacca gttcctgatc ggcattcagc ggctgggaca tcgtaaactc ctgatagcat
     8821 tttgaaagcc gttatagtag cggcttcaca tcttcagcga tagtcacatc caccgtcatc
     8881 aggacacaaa aaaacctgcc ggagcaggtt ttttgttatc ggaacatatt gcctggcagt
     8941 acgtctttga acgtcttgca atagttattg aacatacttt tcaggatttt gcgcagtttc
     9001 atcgcggcac tccgaccatt tgttatacag gtgttattgt ctttgcgctt ataatatgac
     9061 aaccatcaca aaaatcaatc tttatgtgat acaaatcaca taaatacccc tttaatgtta
     9121 taaaaatgat aatcaaaaaa cagcccccct atttctgaca cctacagatg gcaagaaata
     9181 gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct gtgacgcgta aaatagtgct
     9241 ttctcttact cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg
     9301 ctggcggggt gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt
     9361 attgtacggt tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc
     9421 agtcgcggga tcgccgatcg cgcatgccag caggaaggct atacccacgc gatccccttt
     9481 ggtcagccag taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc
     9541 actttgtcat atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca
     9601 gcgaggggat cttcgctgat taaagaaata gcggtaatgc gttttattcc cattcgtatt
     9661 tttaataatt gaagtttata ttt
//
LOCUS       DAECOD010000130         9643 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.130, whole genome shotgun sequence.
ACCESSION   DAECOD010000130 DAECOD010000000
VERSION     DAECOD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.130"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KKO15_003457"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KKO15_003457"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBD6362118.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KKO15_003458"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KKO15_003458"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD6362119.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KKO15_003459"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KKO15_003459"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD6362120.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KKO15_003460"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KKO15_003460"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD6362121.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KKO15_003461"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KKO15_003461"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD6362122.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KKO15_003462"
     CDS             5060..6193
                     /locus_tag="KKO15_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD6362123.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KKO15_003463"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KKO15_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD6362124.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KKO15_003464"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KKO15_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD6362125.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KKO15_003465"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KKO15_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD6362126.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KKO15_003466"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KKO15_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD6362127.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KKO15_003467"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KKO15_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD6362128.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KKO15_003468"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KKO15_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD6362129.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2450 a         2472 c         2459 g         2262 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtcccct gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tct
//
LOCUS       DAECOD010000131         9596 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.131, whole genome shotgun sequence.
ACCESSION   DAECOD010000131 DAECOD010000000
VERSION     DAECOD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.131"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="KKO15_003469"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="KKO15_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBD6362130.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KKO15_003470"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KKO15_003470"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBD6362131.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KKO15_003471"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KKO15_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBD6362132.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="KKO15_003472"
     CDS             complement(2405..2608)
                     /locus_tag="KKO15_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362133.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3780
                     /gene="ddlA"
                     /locus_tag="KKO15_003473"
     CDS             2686..3780
                     /gene="ddlA"
                     /locus_tag="KKO15_003473"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD6362134.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVRDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3804..4016)
                     /locus_tag="KKO15_003474"
     CDS             complement(3804..4016)
                     /locus_tag="KKO15_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBD6362135.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4276..4584
                     /locus_tag="KKO15_003475"
     CDS             4276..4584
                     /locus_tag="KKO15_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBD6362136.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="KKO15_003476"
     CDS             complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="KKO15_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBD6362137.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="KKO15_003477"
     CDS             complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="KKO15_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBD6362138.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7322..8479
                     /gene="ampH"
                     /locus_tag="KKO15_003478"
     CDS             7322..8479
                     /gene="ampH"
                     /locus_tag="KKO15_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBD6362139.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8480..8983)
                     /locus_tag="KKO15_003479"
     CDS             complement(8480..8983)
                     /locus_tag="KKO15_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6362140.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9071..>9596)
                     /locus_tag="KKO15_003480"
     CDS             complement(9071..>9596)
                     /locus_tag="KKO15_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6362141.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2450 c         2284 g         2481 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtccg cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt
     3541 gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta
     3601 accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc
     3661 agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt
     3721 ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa
     3781 tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga
     3841 ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc
     3901 tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg
     3961 ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag atttatggca
     4021 gctccgttaa cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac
     4081 aacattttcg taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga
     4141 agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc
     4201 taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga
     4261 cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat
     4321 cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt
     4381 tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta
     4441 atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt
     4501 ttcgggctga tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg
     4561 cactggcgac atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat
     4621 ctggcctgac attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc
     4681 gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc
     4741 caggcaacat ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt
     4801 tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa
     4861 tttcgctgtc gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc
     4921 cgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg
     4981 ccttactgac tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat
     5041 cagcaaagcg gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac
     5101 caaaccacaa cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc
     5161 acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg
     5221 tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat
     5281 cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac
     5341 ttttgccgtt tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca
     5401 tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg
     5461 gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca
     5521 gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc
     5581 ggtctttcac gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt
     5641 cgcccttttt caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca
     5701 acaatgacag cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg
     5761 tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt
     5821 tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac
     5881 cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg
     5941 caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa
     6001 tgcgggcgat gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg
     6061 cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa
     6121 ccagctcttt acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt
     6181 tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca
     6241 ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta
     6301 ccagcaccgg caggaaggcg atcaacgtca tgatggcgtt gataaaactg acgcccatat
     6361 tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga
     6421 tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga
     6481 acacgtagtg actgacaaag aagttgttca gtacactgat caccaccgcg atcagcgcaa
     6541 tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac
     6601 ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga
     6661 cttccaccaa aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt
     6721 gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt
     6781 agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct
     6841 gcccggaagc gccggtgata cgtgccaccc agtcaccccc accggcttgc cagaagataa
     6901 cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag
     6961 acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc
     7021 gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg
     7081 cttagttcgc cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt
     7141 ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa
     7201 gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca
     7261 atccatcaat gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc
     7321 gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca
     7381 tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga
     7441 tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca
     7501 gcgcgtcttt cgtagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga
     7561 ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt
     7621 gctcgaccag gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc
     7681 acgcgtgccg acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag
     7741 cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac
     7801 gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca
     7861 ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg
     7921 caagccttat acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac
     7981 cacctacacc ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag
     8041 tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga
     8101 tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc
     8161 agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga
     8221 tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg
     8281 tcgtccgagg attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat
     8341 gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt
     8401 taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt
     8461 ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc
     8521 ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag
     8581 aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt
     8641 cttcattcca gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat
     8701 atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat
     8761 cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac
     8821 gcagagagat aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa
     8881 atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat
     8941 cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag
     9001 taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga
     9061 gtgaagaata ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt
     9121 caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact
     9181 gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt
     9241 tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca
     9301 gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca
     9361 tgctgtcgta aggctgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag
     9421 actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga
     9481 agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt
     9541 aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaag
//
LOCUS       DAECOD010000132         9493 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.132, whole genome shotgun sequence.
ACCESSION   DAECOD010000132 DAECOD010000000
VERSION     DAECOD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.132"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..190)
                     /locus_tag="KKO15_003481"
     CDS             complement(<1..190)
                     /locus_tag="KKO15_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBD6362142.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KKO15_003482"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KKO15_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBD6362143.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KKO15_003483"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KKO15_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBD6362144.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KKO15_003484"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KKO15_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBD6362145.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KKO15_003485"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KKO15_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KKO15_003486"
     CDS             complement(3262..3381)
                     /locus_tag="KKO15_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD6362146.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KKO15_003487"
     CDS             complement(3342..3527)
                     /locus_tag="KKO15_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362147.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KKO15_003488"
     CDS             3628..3801
                     /locus_tag="KKO15_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362148.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KKO15_003489"
     CDS             3803..4147
                     /locus_tag="KKO15_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBD6362149.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KKO15_003490"
     CDS             4151..4486
                     /locus_tag="KKO15_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBD6362150.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KKO15_003491"
     CDS             complement(4543..6864)
                     /locus_tag="KKO15_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6362151.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KKO15_003492"
     CDS             complement(6981..7190)
                     /locus_tag="KKO15_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362152.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KKO15_003493"
     CDS             complement(7941..9464)
                     /locus_tag="KKO15_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6362153.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2204 c         2079 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccagtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAECOD010000133         9403 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.133, whole genome shotgun sequence.
ACCESSION   DAECOD010000133 DAECOD010000000
VERSION     DAECOD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.133"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KKO15_003494"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KKO15_003494"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD6362154.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KKO15_003495"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KKO15_003495"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBD6362155.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASMAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKLVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KKO15_003496"
     CDS             3115..3399
                     /locus_tag="KKO15_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD6362156.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KKO15_003497"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KKO15_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBD6362157.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KKO15_003498"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KKO15_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBD6362158.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KKO15_003499"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KKO15_003499"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD6362159.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="KKO15_003500"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="KKO15_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBD6362160.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2250 a         2480 c         2389 g         2284 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag catggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg cagccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa actggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga gcttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ata
//
LOCUS       DAECOD010000134         9378 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.134, whole genome shotgun sequence.
ACCESSION   DAECOD010000134 DAECOD010000000
VERSION     DAECOD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.134"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KKO15_003501"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KKO15_003501"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBD6362161.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KKO15_003502"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KKO15_003502"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBD6362162.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KKO15_003503"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KKO15_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBD6362163.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="KKO15_003504"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="KKO15_003504"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBD6362164.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="KKO15_003505"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="KKO15_003505"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBD6362165.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="KKO15_003506"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="KKO15_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBD6362166.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYTIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="KKO15_003507"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="KKO15_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBD6362167.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="KKO15_003508"
     CDS             complement(8064..9206)
                     /locus_tag="KKO15_003508"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBD6362168.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..>9378)
                     /locus_tag="KKO15_003509"
     CDS             complement(9215..>9378)
                     /locus_tag="KKO15_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBD6362169.1"
                     /translation="YFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFT
                     AIKYNVMKG"
BASE COUNT         2601 a         2170 c         2052 g         2555 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattata ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataat
//
LOCUS       DAECOD010000135         9213 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.135, whole genome shotgun sequence.
ACCESSION   DAECOD010000135 DAECOD010000000
VERSION     DAECOD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.135"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..458)
                     /gene="pgaB"
                     /locus_tag="KKO15_003510"
                     /pseudo
     CDS             complement(1..458)
                     /gene="pgaB"
                     /locus_tag="KKO15_003510"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
     gene            complement(462..1955)
                     /gene="pgaB"
                     /locus_tag="KKO15_003511"
     CDS             complement(462..1955)
                     /gene="pgaB"
                     /locus_tag="KKO15_003511"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBD6362170.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYLSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            complement(1964..4387)
                     /gene="pgaA"
                     /locus_tag="KKO15_003512"
                     /pseudo
     CDS             complement(1964..4387)
                     /gene="pgaA"
                     /locus_tag="KKO15_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            4902..5462
                     /locus_tag="KKO15_003513"
     CDS             4902..5462
                     /locus_tag="KKO15_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBD6362171.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            5405..5794
                     /locus_tag="KKO15_003514"
                     /pseudo
     CDS             5405..5794
                     /locus_tag="KKO15_003514"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            5774..6331
                     /locus_tag="KKO15_003515"
                     /pseudo
     CDS             5774..6331
                     /locus_tag="KKO15_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            6429..8038
                     /locus_tag="KKO15_003516"
                     /pseudo
     CDS             6429..8038
                     /locus_tag="KKO15_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8139..8630)
                     /locus_tag="KKO15_003517"
     CDS             complement(8139..8630)
                     /locus_tag="KKO15_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBD6362172.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(8635..8811)
                     /locus_tag="KKO15_003518"
     CDS             complement(8635..8811)
                     /locus_tag="KKO15_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6362173.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         2608 a         1803 c         1947 g         2855 t
ORIGIN      
        1 ttatgcagaa aattgtcggg ataataacca tagtttttca ctccattcag ttgtaatagg
       61 ctcatccagt gagcgagttg ttgcgaagga atagcctgat gctgaccatt tttctgccag
      121 ttttgtgcct gtaattctaa aatagatttg tctttagcct gagggatgtt tttaatttga
      181 ttggtcaatt gtattaacca ttggtcagcc gatttttctg cgacaccttc catataaggc
      241 atagccataa tagcggtcca gtcatagctt tttaggaaat cagcataatt ctgtgcaaac
      301 caggcttcac tttcaggttg tattaccgga agtgcaaaaa tatttcgtgc agttttaata
      361 tgtggaccgc gaatggcttt tacgcgcgca ctaagttcta aagtgaagtc agttaacgca
      421 cgacttttaa agcgggccca ctgtttaaat tgctccgggt tttatcgaat ttcgctcaga
      481 ctcccgctaa agcctgcttg ctgataagcc gtgatagccg gtgcactggc atcttcataa
      541 tctgaaagca aagcatcatc gtggaacaat atgccatcaa aagcagcatg tccggcaaga
      601 tcttcatata acatgccaac ttgtgctctg actctgtcat cgaaaggaga gagacggtga
      661 tattgttcag gatgaatttg tgcttttttc tcccctgttg gtaagtattt tactcgcgtt
      721 aatgtgggat ctaaatccca gcttaatacc ggcatccacg catagatgtt tacacctgag
      781 cgggtacgta attgccaggc aacccgacta aaaatatctg ctttcattgg tagcaaatga
      841 tttggaaacc agacctcttt gaccagccca tcaccatcgg gatcagcaaa tgcctgcaaa
      901 tacacggttg atatttgcat atctttcacc cgctgaatta gcacatcaat attgcgatcc
      961 atttgctggc ggttttcgtc ataaacgtaa tcaagatcga tatgcattat ccgttgtggt
     1021 gatttttcct gtacggtaat aattttctgg gcaaactctt ttaatgaggg attattggcg
     1081 attaatactc gcggaatgga atccaattgt gacgcatttg ccaaacctga ttcaagggtg
     1141 aaaaacatgt cataaccgag tttttttaat tcctctatcg ctatgccatt cgcttcgcca
     1201 taaggccaaa caaaaacgtg tggatttacc tcagcctttg tacgcaggta ttccgtcatt
     1261 tttacagcat ccagacgaat tctttcccgg tattctgctg cggtttcata ccgtgcgtgg
     1321 tcagtaaaat atgcacgatt tacatataca ggcattaagc tgccggtgga attagcctga
     1381 ataccgtagt gagaattcca tgtatgagaa gcgacctcaa cgagccggga acgcgcaact
     1441 tctcgcactt gttgccacgt ggcaaaatat tctcgatcga ccatctcatc gccaaatttt
     1501 acttgttcat ccactggcgt atcgacccaa ctgccgacgg gggcccatac agcaggccac
     1561 tggaaggcct gaagaattgg gaagacgcgg gtataaaaac tcaggtagcc gtcatcaaaa
     1621 gtcagcacta ctgctttttc cggtagcggt tttcctcctc gatgtgcttc acgaatttga
     1681 gcaatactga ccggttgata accgttctcg cgcagccagg caaattgttc acgcagtgct
     1741 gatgtccgca ctgacataaa acgctggtcg gcagcttcgt cttcaacgtt atgccatgaa
     1801 atcgccacaa aaccattatg cggccacggt tgctcggcga gtaaagattc gcgatcctgt
     1861 ggcggtataa atgatgttct tgactggctg atgcacgcgg tgagcataat tatactcacc
     1921 agcatcagga gatatttatt tccattacgt aacatattta tccttaaaat ctgaatgtca
     1981 tatcgaattc aacgtataag ttgtgttctc tgtcaccgtc ataaggtcgt ttttcccagc
     2041 gtagcgttgc gccagcatca atcacgtcat tccagctaat gcgttgcccg tagccgagtt
     2101 gggtgacgac atccgtgcca taatgttttt gccaggaggc accaacacct gcgctgaaga
     2161 tttgctccca gctattttca tagcttcgcc ataacaaatg acttgcctca aatgccggaa
     2221 caatatcgaa cgtttttata gggttgtagt atggggtatc gtgttctgta ttttgttcgt
     2281 aatacagact gggtaggaaa tcgacaatca aatatggtga agaccagatg cgttcctgac
     2341 cctcaattga gacttcatga cgctggttac tgtcggaaaa atcagtgaaa gcccaggaga
     2401 caccgtactt acgccgctca ttttgatacc agcgaacata agcctgagca ctgttgcctg
     2461 taacaccatt tttcattgcc cgtaatggaa cgcggtgaga gaggcgttcc agttgcgaac
     2521 caatacgcca gttatcatta aaatcatacc agccagacag gcgcgcgccg ggtttatgct
     2581 catgattgaa aaagtgttca gcgtactccg cctcgagcca gatattacgt gaccgccact
     2641 caacacccgc cagccagtcg cgaacaatcc cttttccttc gctaaattgt ccatcggcat
     2701 aaccgaatcc agcaaaaccg cgccagttat ccttcagcgg tggtgaataa acgatggtgg
     2761 ttaagtcgac atcatgttta ccactatccg ggccttcggc atcaattcct gttgaaccag
     2821 ctatacgaag ctctgcaaga ttatgtacat caaccgcacg ttttaaacgt acaacgccgg
     2881 gatcttgcgg ttcacgttcg acaacatcgt gcgttaagac agctgcctgc tgccattctt
     2941 gtaacgttaa tgctgtctag gcttgttcaa cctccagatt aatattacgt ggctcgatca
     3001 cttctgcttt ttttaattca ttttctgctg cacgaggcca accgcgggct tgtaacacac
     3061 tcgcgtaatc aatgcgcagt ccctgatttc ctggtgcgtt ataagcaagc tctctggctg
     3121 tcatttcagc ctgaggaaga tcattactat attttgctac ggttgagaga aacgaatgcc
     3181 cctgtaacta ggtatcattc gggatgctcg taggcgtgcc cattaaccga aggaaaggcg
     3241 gcgaagtatt aatggtatgt tgggtgacag ttagcgcgcc cggataattt tcactctcta
     3301 ggtggctgta aaagagatcc gcaagttctt catcggataa atccggggca atggtctcct
     3361 tgtgataaaa gagctcggtc attattgact gtgctttttt cggctgatga tctttgagat
     3421 aagccgatgc aacccaatat tgcccctagg gcggaataat ttgccccgtc ttttttaatc
     3481 gctgatagtg agaaatgacg tctttataac gatcgcgagt taataacgcg ccaagatgat
     3541 caacctgaat acgctggtac tgggcagtgc ggtctgggtt atcgtgccac agaatttcta
     3601 atgcagcgta ttgggcgagg gcgcgattgg caatggcata acgttcactt tcactgcgcg
     3661 taggcataaa cgacagtctg accagttcgg catgaatatc agcgcgaata tctggcgtta
     3721 aattggcatc gtcaatcgcg gcagcaagtt gattattacg taatgcctgc acgtattctg
     3781 tgggatattg ttgcgtagat gcattttcag gtaatgactc tgtcatcgcc cgtaattcat
     3841 cctgatgacg ccccgccagt ttatagatat aggcttctgt gagtaaattg gctttgtccg
     3901 gtgctccaga gttaagctgc ttaagtttaa ccagcgcagt atcatagtga cccgcatctg
     3961 ccagggttaa aatttgtccc cgttgataat ccttattttg cggctccaga gagagcgcct
     4021 tttgccacag tgtaagcgag ttttgccatt gttgcaggtt acgataagcg acggcgacag
     4081 ctgcataacc acgcgctggt aattgctgat gacggtagcg gttgtaaacg gtaataacct
     4141 gtttatcttg cccggcccat aaggcaatct gtaaccagtc agcaatttgg ttattgctga
     4201 gtgctgattt tagtgcaaac catgacaaag ctggctgagt attacccttg cgagcttcaa
     4261 taatcaatgc atcataggcg ttattaacag cactcttcgc cgcgggactc gctgctaaaa
     4321 atgcggcagt aagaagtttc aaagcccatt tggttttcgg gcaccttttt ctgctacttg
     4381 aatacatcct gtattactcc atgtattgcc aaaatctctc tctgtatcta attacaggta
     4441 actgaaaaga aagatatttt tgcacctcat aatccgttat taaacgcgga agagagacgt
     4501 gaattgttga tgatgagaag aagaaatgat gagcagagtg tccatataaa atccttttct
     4561 cgcccgaaaa tccattccaa tgatgaggat cttcaggaat acggcataaa tcccaatgcc
     4621 tttttcaaaa taaattagga ttaaaataat taaatcagta aattccgatg catgatttcg
     4681 gatttttcga aagtcctgat gaaaggctgc gctacggcac tcacggattt aattgttatt
     4741 cctaatgtat ctatgaatta atgttttata aggattttct ataaccattt ataagtattt
     4801 tcaagcctgg cttgttgcaa acaatgtata aagcacttag gcaataataa ttacattcag
     4861 caactatcat catcggtatt gtttgtgggc ggaatttcat attgacaaca gtacagttct
     4921 tatatctatt aataatagaa agagatctac aacctacaga ttggtgtagc tttatggaaa
     4981 aagactattt gagaattagt agtactgtat tagtgagctt attgtttggg cttgctttgg
     5041 ttttggtgaa tagttggttt aatcagccag gcgttgagga agttgttcca cgatcaacct
     5101 atctgatggt aatgattgct ttatttttta tcgatactgt tgcatttatt tttatgcagt
     5161 tgtatttcat ttatgaccgt aggcaatttt caaactgtat acttagtttg gcttttctga
     5221 gttgtttgat ttactttgtt aaaaccgtca ttatcattca gcaaattatt gaggggcgtt
     5281 tgacaagcag tgttgtccaa aatgacattg caatctatta tttgtttcgt cagatgagtt
     5341 tgtgcatatt aatatttctg gcattggtga ataaagttag tgaaaacaca aaacagcgca
     5401 atttattttc caaaaaaatg actttgtgca taagtttgtt tttgtttttg gggggccgat
     5461 agttgctcac atactttcta gtcattatga gagctacaat ttacacattg ccgagttaac
     5521 caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga ttttcatgtg
     5581 gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata tctggaatgg
     5641 agtgacggtg attgcttttt gtgccgtact gtacaatatc tctctgttat ttatgagcag
     5701 gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta agttaaccgt
     5761 tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga acattgcaca
     5821 tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac tgacagttca
     5881 atcagcatca gccaaaaaaa cgccttattg tgtcatgatt atggatatcg accacttcaa
     5941 agaagtcaac gacacctgag ggcatccggt tggcgatcag gtgataaaaa cagtggtgaa
     6001 tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg gcgaagagtt
     6061 tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg aaaggattcg
     6121 ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac aaaaagtgac
     6181 gattagtatt ggcgctgttg tgactcagga aaatgcgtta aacccgaatg agatctatcg
     6241 actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg tggttgtgag
     6301 ggatgtggtg agtttttgtg agtcaccata aagcggcatt ttagcgggtg attttggtaa
     6361 gcgctaaata gctattcgca gacttgcgta acaattttca gaaatatcat agtactatat
     6421 gatcttaaat gcttatcatt gatcaatctg ctatcacccg cttttactgg caattgtact
     6481 ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta ttcaacaaaa
     6541 aaatgtttgt actcattata ataccaggca ttctgggggt gttactatcg tttgctatgt
     6601 caatatttca aatgaataga gatacgacaa ttaccgccgg tattctgtta aaacagcttg
     6661 ataatgttac gcaaattgtc cagcatacga caaagcttac ctctattctg gtgatgaagc
     6721 cctgcaaaga cattcttgaa cagttgattg ctaacggagc attgactccc tatgttcgaa
     6781 caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt ttcaaaaaaa
     6841 tgaatgttaa cgatgtttac ggtacgagct ttcacaataa aaataaagaa tcacgtatag
     6901 tatcaattag cggtacaagt tttgtgccag gtaaaacagc aatagtgttc cttatgccaa
     6961 tagggaatga tatgacagct tttagtattg tagagtcacg atatatctat gatctaatgg
     7021 atgttcttga tgatgagaat gatgattcat tttctcttcg ttttactgaa gggcctgcaa
     7081 ttattagcgg tgtaaataat aatgacaggt tatatatgtt aaaaagagat tttaattctg
     7141 cgatcagtca ggctcgtctt acagttacta cgccgatgat atcgttatat ccctatatca
     7201 ttagcaatgt gctttatatt ttgccgttat ctattattct ctcttttatt ctctatttct
     7261 tatggcagca ctggatgtca cggaaaatgt cgctggcaga agaaattaaa aaaggaatgt
     7321 catccggaga attttctgtg cattatcaac cagtgtgcga cacgacaacc aaagcgtgtc
     7381 tgggggttga agcgttaatg cgctggcaac gcgaagatgg taaaaatata tctccggtag
     7441 tttttatccg cgctgcagaa gaggaaaatt taataatacc gttaactaaa catctctttg
     7501 aactgattat tcaggatgta caaagctgga aagtgaaaaa gccgtttcac cttgggataa
     7561 atattgccgc ggaacatctt gcacatcctg actttgttgg tgatgtttta catattaaaa
     7621 atgcaatatc tgataaattt aatattgtcc ttgagattac cgaacgcaat ctggttgaag
     7681 acactgatct tgcattacag aaaataaatg aattaaggat ccatggctgt gaatttgcag
     7741 tcgatgattt tggcacggga tattgttccc ttggccttct gcaaaagttg tctgttgatt
     7801 atcttaaaat agataaaagc tttatagaca cacttactac agcggaagac gaaacgccag
     7861 ttttagatat tattattaag ctgagtaatc ggctcaattt gataacgatt gcagaaggtg
     7921 tcagtactcc tcaccagcag aatatcttat tcataataac gtgacattag ttcagggata
     7981 cttatatgaa aaaccaatga aagcgacaga gttttatcag tggtatgtaa acagataaat
     8041 aattaaaaat attggaatgg ttttgtattg tcttaaaata taaacaaagc aatcagaatg
     8101 ttattaactg atgacaaacg ccaggatcaa caatgctgtt agtgagtaat acatatgaat
     8161 cgtggtgtcc tgcctgtaga gaatatgtag ttataatcat tttgacgcgt aacataataa
     8221 ttaattgaag tgtctgcttt tatgaagaaa tcagaagtga tattttcaag gatttcatcg
     8281 ctgatgttat catctttttg tattttttgg attttaactg tggtgagata aacggtatca
     8341 cggaatttct tgtatgtaaa actcacatat cggcggatga agcccatttt ttttccatct
     8401 accgtgtatg cgcccgtgag atacatgttt cctgtgttat tttcagaaat catatttaac
     8461 gaaaaatatt gatccatttt tgtatgatcg gtgaaattaa taacatgggc attacatgat
     8521 atgttttgct ctgctggttc attggaatga tgaaacgcaa tatggctcag aattgctgca
     8581 ataaaagcac ataaaattat tcccgaaaac agaaaatatc tttgagtcat atgattactc
     8641 cgctttataa tagagagagt tgcaattatt tggtgattca gagtttaaca tttgactaca
     8701 ggtgaaagca taaaacccgc tcatgctatc atttgttgca atgtaaatgt aaggatgtat
     8761 tttgcagttg acttccattt tctgcaattt ttcaataatt ggtgatagca tgtctacgga
     8821 attaaagtaa ttattatcat agaaataact gcatccttcc tgagtagggt gttgagcata
     8881 atttgagaaa gcatctattt tttttatagt gtgagatttg ctttgaaaag ccatatcgaa
     8941 aaaataagaa gacatataaa ctatccccca tgccgcaaaa aaaaactata agtaatgtat
     9001 tctttgtggt aaatatacct tttgcaacgt aatttttatt tatattttca gtgtgggtgc
     9061 cattctcttc actctcaaca ctttcattct cattgtgatt attgtcgaga acagttatgt
     9121 tgatttcttc attgattttg aagcctttcc gtggcaccgt tctgataact gatcctaccc
     9181 acgtaatatg gacacaggcc taagcgaggt tct
//
LOCUS       DAECOD010000136         9170 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.136, whole genome shotgun sequence.
ACCESSION   DAECOD010000136 DAECOD010000000
VERSION     DAECOD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.136"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="KKO15_003519"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="KKO15_003519"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBD6362174.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="KKO15_003520"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="KKO15_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBD6362175.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="KKO15_003521"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="KKO15_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBD6362176.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="KKO15_003522"
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="KKO15_003522"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBD6362177.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="KKO15_003523"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="KKO15_003523"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBD6362178.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /locus_tag="KKO15_003524"
     CDS             complement(7908..8219)
                     /locus_tag="KKO15_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6362179.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="KKO15_003525"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="KKO15_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBD6362180.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="KKO15_003526"
     CDS             complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="KKO15_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBD6362181.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQCGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT         2297 a         2368 c         2180 g         2325 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtgattttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgttgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctaaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aaccagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacatcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtggctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgcaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc tgccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taactataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcccca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacattgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattattac tgaggtgtac tggcaatagc ggacactacc atttgttctt
     9121 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat
//
LOCUS       DAECOD010000137         9130 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.137, whole genome shotgun sequence.
ACCESSION   DAECOD010000137 DAECOD010000000
VERSION     DAECOD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.137"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            24..840
                     /locus_tag="KKO15_003527"
                     /pseudo
     CDS             24..840
                     /locus_tag="KKO15_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(844..2234)
                     /gene="tcyP"
                     /locus_tag="KKO15_003528"
                     /pseudo
     CDS             complement(844..2234)
                     /gene="tcyP"
                     /locus_tag="KKO15_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(2367..2957)
                     /locus_tag="KKO15_003529"
     CDS             complement(2367..2957)
                     /locus_tag="KKO15_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6362182.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3120..3788)
                     /gene="hxpB"
                     /locus_tag="KKO15_003530"
     CDS             complement(3120..3788)
                     /gene="hxpB"
                     /locus_tag="KKO15_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBD6362183.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3935..4471
                     /locus_tag="KKO15_003531"
     CDS             3935..4471
                     /locus_tag="KKO15_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBD6362184.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4512..5372)
                     /locus_tag="KKO15_003532"
     CDS             complement(4512..5372)
                     /locus_tag="KKO15_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBD6362185.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5478..5768)
                     /gene="ghoS"
                     /locus_tag="KKO15_003533"
     CDS             complement(5478..5768)
                     /gene="ghoS"
                     /locus_tag="KKO15_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD6362186.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5869..6798)
                     /gene="pfkB"
                     /locus_tag="KKO15_003534"
     CDS             complement(5869..6798)
                     /gene="pfkB"
                     /locus_tag="KKO15_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBD6362187.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7085..7843
                     /locus_tag="KKO15_003535"
     CDS             7085..7843
                     /locus_tag="KKO15_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBD6362188.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7896..8003)
                     /locus_tag="KKO15_003536"
     CDS             complement(7896..8003)
                     /locus_tag="KKO15_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362189.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8175..9130)
                     /gene="espL1"
                     /locus_tag="KKO15_003537"
                     /pseudo
     CDS             complement(8175..9130)
                     /gene="espL1"
                     /locus_tag="KKO15_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT         2433 a         2122 c         2153 g         2422 t
ORIGIN      
        1 aagataataa ttttgttaac aatatgaggc gggagatgtt ttctacaaac ttaatacaga
       61 gtaattatgg tgatttaaat attaaaagcc tggctttcga ctcttttaaa gaaaggcttg
      121 agtctaccat ggcggcactc actttcttta tatcaacggg tcaatgtgac tgcgatgaaa
      181 tagcagagtc gaacttcaat cgtatgattg cctatatgtc taacattaac tatgatgcct
      241 caaagctagg agcgccagca cttagttttg atacgtatct gcaggacaat gttaaatata
      301 gagtgataat taataatctt tatggcagtg agtttcggat aagagatatt aatgagaatt
      361 ttgttgggat ggattttacc tgtgaattcc gacccgaaaa aatgacgaat ttgatcaata
      421 taaaaaatag gttggttatt cattatttaa ggactatata ctatgaaaaa aattatatac
      481 atcctgccgg gagcattttt gctgcgattc aaaaaaaatg agagtctcct aaagtttcct
      541 tcgataatat caatgcttaa tatcaatctt ctttttaatc cactaaatct tccgggaatg
      601 ggaagtggtg tgcttgaaga tattatgagt attccagata gttcacttag aaaacgtctc
      661 gggtatgagg ttttaagctt ctcactacag gcacattcat tgagccagga atgtattgat
      721 aagttggaca tttttttcgc tgacgatctg tttaaatatg agtcagtatg catcgccgcg
      781 atggaacatt taaaaagtaa agcaacggcc cctatacaaa acggaccgtt gccagcataa
      841 gaattaacgg tgtgccagtt cggcgtcgtc ttcgctatcc agaatggctt tatcggtttg
      901 cttcagccac tggctggtca gcgtgccagc tgtcatcgag ccactaacgt ttagcgccgt
      961 acggcccatg tcgataagcg gttcaacgga gattaacagc gccaccaggg ttactggcag
     1021 gcccatcgca ggcagcacaa tcagtgcggc gaaagttgca ccaccaccga caccggcaac
     1081 gcctgcggaa ctaacggtaa caataccgac cagcgtcgca atccacatcg ggtccagcgg
     1141 gttaatgcca accgtaggcg caaccatcac cgcagcattg ccggatacaa accggcgcag
     1201 ccgttctgac caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaacg
     1261 cccagacgac gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg
     1321 gtaaaggcaa acgtcagcac aggccatacc ttacggaagt acttcagcgg actcacgcca
     1381 ttaatgccca gcagaatgcc atgcacagca aacataatca gcagaccgag gtaggacgcg
     1441 acaacgaaac ttcccagttt gatgatgtct tgcaagttag aacctgcaac cactttggtc
     1501 attagagcca gaacgccgta aggggtcaac tgcatgacaa ggcgaaccag tttcatcacc
     1561 cagctttgta gggtatcgat agcggttaag acgcgttccc ctttcggcgc atcatccttc
     1621 agcagtttca atgcagctac gccgaggaat gcggcaaaaa ttaccacgct gataattgac
     1681 gtcggattgg ctccggtaag atcggcaaac gggtttttcg ggataaagga caagaccagc
     1741 tgcggaacgc tctgatcaga gactttacca acatagttac tttcaatggc gttcagacgt
     1801 gcagtttctg caccaccctg aaccagacct tcagccgtca aaccaaacag gttggtgacc
     1861 agcacaccga ccagcgccgc aatcagcgtg gtaaacaaaa gcgtaccgat ggtcagaaaa
     1921 ctgattttgc ctaactgaga tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg
     1981 aagactaacg gcataacgat catttgcagc agttgaacat agccgttacc aacgatgtta
     2041 aaccactgta cagaatcttt aagtacctgg ctgtcagaac cataaatggt atgcagggca
     2101 aggccaaaaa ccacacccat caccagaccc accagcactt ttttcgccag actccactgt
     2161 ttatggcggg tctgagccag cgcaaacagc agtacaacga acaccacgat gttcgcaatt
     2221 aatggaaagt tcatccccgt tctcctgatc ttattatttc tatcactgcg tagcagtcat
     2281 agtgttggcg caagattaac agaactgtga gctggctctt atattcaaat ggaattgctt
     2341 attccagaaa ggtgattttt gcctttttag tattcaacct gatgcttaat aagccgatga
     2401 aactggatct gcaaggtatt gatcatttgc gatgaccaac gaacagcgct gttctcgggt
     2461 aatgaatggg gcatccatac cgaccagaca aataatgcca tgcagataaa acgttccagt
     2521 tggttgccct tttggggaac caggataggc aagcggaaac gccagcggca tggccagagc
     2581 aggggaacac cggcgggtgt cagcatatcg gcaagtatgt ggctcaaata acccagcacc
     2641 attccttgta gcgcatcagc cggaatgaac cagccttccg gaaccttaag gtaaaaggtt
     2701 gccagcagcg caaataccgc cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg
     2761 gcgatcggtt ttgatatcca ttttaatcgc tgcccaagaa acgactttgg gtgatcgatg
     2821 tccggtaaca aacacgtcag gattgcggaa gggacaatat gccaccagtc accctgtgcc
     2881 agcacgggcg tcagctcggc atttttggca aataccgcac aagcaataga aaagagaagg
     2941 tgaccttccg ccgtcatgat aaaactcact aaactgtcga tacgtacagt ataggatttt
     3001 tatacagtgc acggaagtgg tgacggtgta actctttctc ttaaattgac gattaatccg
     3061 tcagttgaat gcaaattcag ataaagaacc aggggcagcg tgatcactgc ccctggtcat
     3121 taaccgagaa ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat
     3181 acaaaacgtg gatcattttg cgcttctggc gcaggaacga cgatggaacg catgcgggct
     3241 gctttagaag cgatcatgcc atttaccgag tcttccagcg ccacgcaggt cagtgggtca
     3301 acgcccagtt ttgctgcgca gtcgagatat acttgcggat gcggcttgct gtaaggcagt
     3361 ttttcagccg aggcgagggc atcgaaactg tcgcgtaagt caaacatggt caacactttt
     3421 tccagcatat gtagtggtga cgcggaggcc agtcccacca ataaaccttg ttctttgcat
     3481 aacgcaacag cttcgcgcac gcctggtaat aatggacgtg tctcttcaac cagtgaaatg
     3541 gcacgggcaa taacccgttc tactacttcc tgacggcttg gcccattcca cggttgccgg
     3601 gcgtaccaaa gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta
     3661 cgacgggaga tatctacccc caggcttgcc atcacatcca gttcggctcg atcccataaa
     3721 ggttctgagt cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg
     3781 gttgacatta caacctctcc tttagagcat tatgtgtaag gtattaagcc gtgcttatga
     3841 ttaagcgcct atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact
     3901 aagtgaaacg acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat
     3961 tgctgttttg aaaaggattt tgggttgggt gatttttata cctgcattga tctctacatt
     4021 gatttctttg ctgaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt
     4081 catgctcgac tttgctcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa
     4141 tctgttctgg tataactccc cgacccctaa ttttaatggt ggtgtgaacg ttatgttctg
     4201 ggtgattttc atcctgattt ttgtcggact ggcgttgcag gattccggtg cccggatgag
     4261 tcgccaggcg cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa
     4321 aggggaagag gggctaacgc gtgaacaaat tgagtcccgt attgttgttc cgcaccatac
     4381 tattttcctg cagtttttct cactatatat cctgccggtt atttgtattg ctgcaggtta
     4441 tgtgttcttt tctctgcttg ggtttattta atcaaaaggc cgcacggagc ggccattatc
     4501 ctcaacccat atcatgctgc taataatcta tccaatgact gctgagcaat aaccaaatgc
     4561 tgaccgccaa ataatcttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt
     4621 tcaaggaaat cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc
     4681 tgttcagtat gcagcggtaa catcgctagg tcgcactctc ggtcacccca gtcgcaggca
     4741 gggtcgaaaa tgtacgggcc atccggaccc agcgcacagt tgccggacca taaatcgccg
     4801 tgcaacagag aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctcgacgatg
     4861 gcgtcgatat tgccgaaagc gatccctttc tctgctgcca gttccaactg ccagccaatc
     4921 cgttgttcag caaaaaacgt tgaccagcga cgttgccagg tgttgggctg tggagttgtg
     4981 gagagcgagt tatcaaaatc gaggccaaat tgtggttggt cactccattg atgtaaacgc
     5041 gcaatttgct gaccaagaat aaatgcgcta tgcgcatcca gcggacgagg tgggagataa
     5101 tccatcacca gaaaactgta gtcacggtca gcgcctactg cccaaacctt aggcacggtg
     5161 acggttttac tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc
     5221 agttcccttt catcacattt gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg
     5281 gcatggactt ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc
     5341 tcgctcaaaa gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat
     5401 cataaggtta gcgcaagaat gcggtgtaaa aacacgagct aacgcacacc acagactgat
     5461 tattgcccaa aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggtg
     5521 atttcaggat ctttgccggt tgcactttgg gtaagccccg aaaccagttc tctaatttct
     5581 tcttcacttt gggtactgat aagtccaaaa gtgttagtcc ccagctcatg gatatttccc
     5641 tcatcatccg tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta
     5701 atttcagtca atgtatcctc atgcaacatg acggttatga cataacggac aagatcgcca
     5761 ctggccatag ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt
     5821 tgccgacagg ttggtggtga ttcccccaat gctgggggaa tgtttttgtt agcgggaaag
     5881 gtaagcgtaa attttttgcg tatcgtcatg ggagcacaga cgtgttccct gattgagtgt
     5941 ggctgcactc cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc
     6001 cagtttcagt gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtac tctggctttt
     6061 caccggtggt ggcaccacct gaatgcagtt ttcactatcg acacccagcg ctccttgtgg
     6121 accaagggaa acgacaaccc gtttggcctt gccgctatta acgatttcct gcgcggcttt
     6181 gcggacatcg tctggctggg tgagttcgcg attcaccagc gcactgagtt ctttttggtt
     6241 aggcttaacc aactcgatgt taccaatcgc cagtgctgca cttaacgctt cgccagaact
     6301 gtcgatgatg cagcggatcc cttggttttg cgcagcggaa atcagttggg ttaatttttc
     6361 cagcttcaca cctggcggca ggcttccgct tatgaccagg atggccccgg attcaatttc
     6421 cagaacttgc tcttcaagct ggcgaaactc atcttcattt aatgccgcgc ctggcataac
     6481 aaaacgatac tgttcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc
     6541 tttggcttct acagtagcga cggggacatt ttcatccgcc aacagtgaaa ccaggtgttc
     6601 gccggtcgcg ccacccgccg ggaagatcgc tgtggcactg cctccaagat gggcaatggc
     6661 gcgggcgacg ttgatgccgc cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt
     6721 tccttcggga taaatttgcg gggtaattgt tgcgctatcg agagagggcg caagtgtcaa
     6781 cgtatagata cgtaccatca tttcctccta taggctgatt tcagtctggc accgaatcag
     6841 cggaaagtga agaaattaac aatatgattt attagagctt tatttaaaat tttgcagata
     6901 aatatatata aataaaaatc tgttttcgcc acatgcttat tcaaaaaatg aaataagaat
     6961 acattgctaa gttattcgcg cctttttata attcgttacc tttccaggga cgctaatctt
     7021 tgatccttaa gaaagtttcc gggaccgcga tggtctcttt ttttgttgta cggactcttg
     7081 ataaatgaag cttttgaaga cagttcctgc catcgttatg ttggcggggg gcatgtttgc
     7141 gtcactgaat gcagctgccg atgattccgt ttttactgtc atggacgatc ctgccagcgc
     7201 taaaaaacct ttcgaaggta atctgaacgc aggatatctt gcacaatcag gcaatacgaa
     7261 aagctcctca cttacggctg acaccaccat gacctggtat ggacaaacca ctgcctggtc
     7321 gctgtggggg aacgccagta atacttcttc taatgatgag cgttcttcag aaaaatatgc
     7381 ggcgggcggg cgtagccgct ttaatttaac tgattatgac tatttatttg gtcaggcaag
     7441 ctggctgaca gaccgttata acggctatcg agagcgcgat gtgttgaccg cgggttatgg
     7501 tcgtcagttt ttgaatggcc cggtacacag tttccgcttt gaattcggtc caggtgtgcg
     7561 ttacgacaaa tataccgaca atgccagtga aacccagccg ctgggctatg cctccggggc
     7621 ttatgcatgg cagttgactg acaacgctaa atttacgcag ggtgtttcgg tctttggcgc
     7681 ggaagacaca acgctgaact ccgaaagcgc attaaatgtt gctattaatg aacattttgg
     7741 actgaaggtg gcttacaacg tcacatggaa ctctgaacct ccagagtctg caccggaaca
     7801 taccgaccgt cggacaacct tatcgctggg ctacagcatg taaaattatc tgccggaccg
     7861 tgttatgcgg tctggctgtc ttcgtttgtc actgctcagc cccaaatcat tgctgcccaa
     7921 cgtagcaaca acatagcgcc acagattgcc agtagcacca ccaccatctt ccagtgtttt
     7981 ttatcaaagc gtttagtcgg cataccagtg tcctgcatat ctcttgtgcg tgataacgga
     8041 gtgtaacaaa agggacaggc atcaagggat tttttgttat tacagccgtc gggtggcgtt
     8101 gtgcacatgg tcactgtttc tgcgttttat gaaaaaaata aaattatgta tcgatatata
     8161 tcgtaaatag ctggttataa atacgtcttt atttcatcag cactaaaact caggctttgg
     8221 ctgatgacat ttatagcagc ataatatgtt tctacagttt tataatgctt atgatgaatg
     8281 gctatatgaa cagctgacat gttgtcatga tttttagcgg ccaatagtgt aaataactga
     8341 tgttgactaa aagaatgttt ttttgcaaaa gcacccagcg tcgatatata agataacacg
     8401 acgtcttcat tacccttgct catggcgatg tataatgcag gggttccctg tgctgtagct
     8461 ccttttaata aatccatgat gttagctttg ctcaacttgt atttaaaggc gatgccatta
     8521 attttagaga ggaaccgtgt gacacacaaa gggtgattat tttccattgc ggcgtataat
     8581 cctggctcac catcatgtat tttcatggct aaaatactca tcaccagttt tttgtttaaa
     8641 agctcttagt gctattggag tggttaatgt attcagaaag atatcgatta tttcatcgaa
     8701 tccatacaac atggagatat aaagtccagg tgtttcgtta gcacttttag tttgcagtaa
     8761 tttaaccaga ttatcttcat gaatgatgtg attatctacc agtgactgaa tttcttgcat
     8821 aaatattttc acgttgtcag tatgtccgtt catcatagcc aaaaataaag caggtgtacc
     8881 aacgctattc tgcgcagtta ttatttcaat gatttctttt tcagggagta tgggcaactg
     8941 tttgaataat gtaataagct gttgagtctg gcctgtagcc attattaaca acattgccga
     9001 tggatgtata ggggcaaatg gcattcgtga tgcaataccc gcgaaaatag cccctggaac
     9061 tcccacaggt aataggggca gaatagcaat tgcacagttg cgaacatacc tatcagccca
     9121 ggtctgagca
//
LOCUS       DAECOD010000138         9096 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.138, whole genome shotgun sequence.
ACCESSION   DAECOD010000138 DAECOD010000000
VERSION     DAECOD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.138"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KKO15_003538"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KKO15_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBD6362190.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KKO15_003539"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KKO15_003539"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBD6362191.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KKO15_003540"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KKO15_003540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBD6362192.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KKO15_003541"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KKO15_003541"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBD6362193.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="KKO15_003542"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="KKO15_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD6362194.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="KKO15_003543"
     CDS             5545..6018
                     /locus_tag="KKO15_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBD6362195.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="KKO15_003544"
     CDS             6047..6589
                     /locus_tag="KKO15_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBD6362196.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="KKO15_003545"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="KKO15_003545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBD6362197.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="KKO15_003546"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="KKO15_003546"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBD6362198.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="KKO15_003547"
     CDS             7986..8243
                     /locus_tag="KKO15_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBD6362199.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="KKO15_003548"
     CDS             complement(8219..8773)
                     /locus_tag="KKO15_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBD6362200.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2282 a         2209 c         2270 g         2335 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
     9061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAECOD010000139         9086 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.139, whole genome shotgun sequence.
ACCESSION   DAECOD010000139 DAECOD010000000
VERSION     DAECOD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.139"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..923
                     /locus_tag="KKO15_003549"
     CDS             <1..923
                     /locus_tag="KKO15_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6362201.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="KKO15_003550"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="KKO15_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBD6362202.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KKO15_003551"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KKO15_003551"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBD6362203.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="KKO15_003552"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="KKO15_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBD6362204.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="KKO15_003553"
     CDS             4446..4805
                     /locus_tag="KKO15_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBD6362205.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="KKO15_003554"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="KKO15_003554"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBD6362206.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="KKO15_003555"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="KKO15_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBD6362207.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="KKO15_003556"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="KKO15_003556"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD6362208.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2068 a         2203 c         2455 g         2360 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcacc
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gcgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg ccgggtcagc ggggtt
//
LOCUS       DAECOD010000140         8940 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.140, whole genome shotgun sequence.
ACCESSION   DAECOD010000140 DAECOD010000000
VERSION     DAECOD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.140"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..2887
                     /locus_tag="KKO15_003557"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KKO15_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KKO15_003558"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KKO15_003558"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBD6362209.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KKO15_003559"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KKO15_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KKO15_003560"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KKO15_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KKO15_003561"
     CDS             complement(7280..8041)
                     /locus_tag="KKO15_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBD6362210.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KKO15_003562"
     CDS             complement(8038..8697)
                     /locus_tag="KKO15_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD6362211.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2180 a         2369 c         2329 g         2062 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg aggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtttcc cccgttactt tattcgtacc ccttataatg gggtgttagc
//
LOCUS       DAECOD010000141         8762 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.141, whole genome shotgun sequence.
ACCESSION   DAECOD010000141 DAECOD010000000
VERSION     DAECOD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.141"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            5..436
                     /locus_tag="KKO15_003563"
     CDS             5..436
                     /locus_tag="KKO15_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBD6362212.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KKO15_003564"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KKO15_003564"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBD6362213.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KKO15_003565"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KKO15_003565"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBD6362214.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KKO15_003566"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KKO15_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBD6362215.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="KKO15_003567"
     CDS             complement(4438..5145)
                     /locus_tag="KKO15_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBD6362216.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KKO15_003568"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KKO15_003568"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBD6362217.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="KKO15_003569"
     CDS             complement(6003..6206)
                     /locus_tag="KKO15_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6362218.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="KKO15_003570"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="KKO15_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8762
                     /locus_tag="KKO15_003571"
     CDS             7870..>8762
                     /locus_tag="KKO15_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD6362219.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2033 a         2389 c         2251 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtga
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgcgaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 ca
//
LOCUS       DAECOD010000142         8623 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.142, whole genome shotgun sequence.
ACCESSION   DAECOD010000142 DAECOD010000000
VERSION     DAECOD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.142"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KKO15_003572"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KKO15_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBD6362220.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3161)
                     /gene="hemX"
                     /locus_tag="KKO15_003573"
     CDS             complement(1968..3161)
                     /gene="hemX"
                     /locus_tag="KKO15_003573"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD6362221.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQGDTPAA
                     APQGE"
     gene            complement(3183..3923)
                     /gene="hemD"
                     /locus_tag="KKO15_003574"
     CDS             complement(3183..3923)
                     /gene="hemD"
                     /locus_tag="KKO15_003574"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBD6362222.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3920..4861)
                     /gene="hemC"
                     /locus_tag="KKO15_003575"
     CDS             complement(3920..4861)
                     /gene="hemC"
                     /locus_tag="KKO15_003575"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBD6362223.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5248..7794
                     /gene="cyaA"
                     /locus_tag="KKO15_003576"
     CDS             5248..7794
                     /gene="cyaA"
                     /locus_tag="KKO15_003576"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBD6362224.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7834..8154)
                     /gene="cyaY"
                     /locus_tag="KKO15_003577"
     CDS             complement(7834..8154)
                     /gene="cyaY"
                     /locus_tag="KKO15_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBD6362225.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8202..>8623)
                     /locus_tag="KKO15_003578"
     CDS             complement(8202..>8623)
                     /locus_tag="KKO15_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD6362226.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1964 a         2230 c         2213 g         2216 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgt agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtttagct
     2041 tccgttgtcc ccgctgccgg ttgtgccagc aggttacgca cgcgagtctg catcagtttt
     2101 tccagcatcg cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt
     2161 aactggtcca cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagca
     2221 cgtacccagg tggagacgtt ctccagcgcc tggcgataag tctcttcctg atgacgcggt
     2281 acagcttgtg ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga
     2341 tttggcgcta acagcggtac ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc
     2401 ataaagttct gccagctttt ttgcagattg atacgccatt cgctgatgga actggaaagc
     2461 tcttcaccgt ctgaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta
     2521 tctacctgat ttgaaagctg attaagctta aggatgatgc cgtcataatc cacctgcgat
     2581 actgcagaaa ggctggcgat atcatcggta attgcccgac gaacggtaat cagactcggg
     2641 tcattcatat ccgccaggct ggcgtctgca cttttcagca acgctgcaga ggtcgtgacg
     2701 tcctgatcgc tccacagctt ccgtccggcg agtttcacca gaaaatcggc ctgagccagc
     2761 agccaggttt tagcatcgct gccggaaatg gtggcgatct tttgttggac ttcatccaac
     2821 tgttttgcca gcgtttcttg ctgacgattc gcctgcttaa gctgcgcagc ttgttgctta
     2881 ataatgcctt ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg
     2941 ttagccaggg catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac
     3001 aaaccgacac ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcagta
     3061 ttgttcttac tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg
     3121 gtctcttcaa ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag
     3181 agttattgta atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct
     3241 tgccagccca gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag
     3301 tgtagtaacc agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt
     3361 tcaccgctgg taacaacgac catcgtcacc tcgcgggatt gccagcgcat cgcttcttct
     3421 gcaccatcgt aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg
     3481 cgcaccgtca gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca
     3541 cgtttgcccg caatattttg taattcaggt aattgtagca agacttcgct gatttcccga
     3601 tcctgtgggt agagaatctt ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt
     3661 ccaatggcga aataagtagg tagttggggc catttacgat cttgctgatg cagctgtgat
     3721 tgggcaaaag caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct
     3781 gccagttgat cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa
     3841 tgccaggcca cctgccccag tgtgcgcaga cggctcacta actcttctcc agcgggagac
     3901 gggcgggtga ccaggatact catgccgggg cctctccgtt atagacttca gcgaggatct
     3961 cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatctt
     4021 gcggcgcacc gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg
     4081 cacatagcca gatttcgcca tcaataagct cggcgtagct accaattggc acctgacatc
     4141 cgccttcgag acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt
     4201 gattcagcgc ggcaagcagc tcgcgagtgc gtgtatcatc aaggcggcat tcaataccga
     4261 ctgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg
     4321 actccagacc taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt
     4381 tatctagttt gctcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg
     4441 ggcggcgttc agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg
     4501 ctggtaacgc atccagactg tcatagttat tggacacaaa ggcatcgcga ggatcttcac
     4561 gctcgcaaat agtgaccagc cccagacctt gcgggaattc aaccggcaca tctttcattg
     4621 aatgtacggc gatatcagcg cgattctcca ggagcgcgac ttccagctct tttacaaata
     4681 agccttttcc gcctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca
     4741 tcggtaccag ttcaacgacc aggcccggat ggctcgccat caacttgtct ttgacatagt
     4801 gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca
     4861 tgcttgttac cgtcattatc atccgtggtc catcctaaca tccttgccag agtgatgtca
     4921 gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc
     4981 tgacaggcgt ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt
     5041 gtatgtagcg catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag
     5101 accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt
     5161 gacatacgaa atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat
     5221 gacgggtagc aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga
     5281 ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc
     5341 caacaggtct acagtctact gccgacattg ttgcactatc accatccgct aatgccgggt
     5401 taccttgatg gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc
     5461 cactacctga acgagcttga actgtatcgt ggaatgtcag tacaggatcc accgaaaggt
     5521 gagcttccaa ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt
     5581 tcctctgacc tggatatctg ggtctgtcat caatcctggc tcgatagcga agagcgccag
     5641 ttactacaac gtaaatgcag cctgctggaa agctgggccg cctcgctggg tgtggaagtc
     5701 agcttcttcc tgattgatga aaaccgcttc cgtcataatg aaagcggcag cctggggggc
     5761 gaagattgtg gctccaccca gcatatactg ctgcttgacg aattttatcg tactgccgtg
     5821 cgtctcgccg gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agagcattac
     5881 gacgactatg tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat
     5941 ctcggtggct taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc
     6001 tacaagagta tcgattcccc atacaaagcg gtgctgaaaa cactgctgct ggaagcctat
     6061 tcctgggaat acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac
     6121 ggcgagattg tatcgtttgg cctcgatcca tactgcatga tgctggagcg tgttactgaa
     6181 tacctgacgg cgattgaaga tttcacccgt ctggatttag tacgtcgctg cttctattta
     6241 aaagtgtgcg aaaaactcag ccgtgaacgc gcctgtgtag gctggcgtcg cgcagtgttg
     6301 agccagttag tgagcgagtg gggttgggac gaagctcgtc tggcaatgct cgataaccgt
     6361 gctaactgga agattgatca ggtgcgtgag gcgcacaacg agctgctcga cgcgatgatg
     6421 cagagctacc gtaatctgat ccgctttgcg cgtcgcaata accttagcgt ctccgccagt
     6481 ccgcaggata tcggcgtact gacgcgtaaa ctgtatgccg cgtttgaagc attgccaggt
     6541 aaagtgacgc tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt
     6601 atttatgtgc cgccgggccg ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca
     6661 aatattgagt cgatcatcag ccatcagccg ctggaatata accgttacct gaataaactg
     6721 gtggcgtggg catggtttaa cggcctgctg acctcgcgca cccgtttgta tattaaaggt
     6781 aacggcattg tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc gcaccatttc
     6841 ccgctgcgct tacctgcacc gacaccgaag gcgctctaca gcccgtgtga gattcgccat
     6901 ctggcgatta tcgttaacct ggaatatgac ccgacagctg cgttccgcaa tcaggtggtg
     6961 cattttgatt tccgtaagct ggatgtcttc agctttggcg agaatcaaaa ttgcctggta
     7021 ggtagcgttg acctgctgta ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac
     7081 ggcgagcaat cgatgatcga agccctgaaa actattctcg gcaaaatgca tcaggacgcc
     7141 gcaccgccag atagcgtgga agtcttctgt tatagccagc atctgcgcgg cttaatccgt
     7201 actcgcgtgc agcaactggt ttctgagtgt attgaactgc gcctttccag cacccgccag
     7261 gaaaccgggc gtttcaaggc gctgcgcgtt tctggtcaaa cctggggctt gttcttcgaa
     7321 cgcctgaatg tatcggtaca gaagctggag aacgccatcg agttttatgg cgctatttcg
     7381 cataacaaac tgcacggctt gtcagtgcag gttgaaacca atcacgtcaa attaccggcg
     7441 gtggtggacg gctttgccag cgaagggatc atccagttct ttttcgaaga aacgcaagac
     7501 gagaatggct ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac
     7561 tgcgaaggca gcaaagagga gctggtacgt gacgtcagtc gcttctactc gtcatcgcac
     7621 gaccgcttca cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt
     7681 gtgaaggttg atggtcgcga acaggtgatt ccgttccgaa caaaatcgat tggcaacctg
     7741 ccgcccgcca atcaggatca cgatacgccg ctattacagc agtatttttc gtgatgagcg
     7801 tgccggaaag cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc
     7861 ctgttgcgtc gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat
     7921 ccattcatcg cctttcaggt caaaatggta gccgccctgt ttggttgcca gccacacctg
     7981 gtgcagcggc tcctggcggt taataatgat tttgctgccg ttttcaaagg taatggtcag
     8041 tacgccgccg ttgatttcgc agtcgatatc gctgtcgcca tccctgtcgt ccagacgctc
     8101 ttcaatggtc agccacagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt
     8161 atcctgtttt taagtgatgg cggcagtata gcggcatggg gtcagggctt caaagtttgc
     8221 acctctgctg ctgcgttccg gcatgattca tccgtcaccg gaataatgat gtctctgttt
     8281 tgaacttcac gtacgttctc ctctctgaca cgagttttcg ccgggcaggg ggagggcgta
     8341 atccttacct ctgtatcggt ccatgtataa acattgcgat cgtcataagc gaagcgatcg
     8401 tttttagtta gcgaaccgtt aactggaata atttgcaagc tctttgaccg taattggcgg
     8461 ataaagtaag catgctgttt gccgctatta aatgagcgat caatgaccag gtctggcgta
     8521 tccagcggga ttatctcaag ctggtcttca ccaaatgtcg ataattttgt tacatacagc
     8581 cgatctttat attgcgcaat attgccagta atcagatgga att
//
LOCUS       DAECOD010000143         8425 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.143, whole genome shotgun sequence.
ACCESSION   DAECOD010000143 DAECOD010000000
VERSION     DAECOD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.143"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            44..397
                     /locus_tag="KKO15_003579"
     CDS             44..397
                     /locus_tag="KKO15_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBD6362227.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="KKO15_003580"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="KKO15_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBD6362228.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="KKO15_003581"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="KKO15_003581"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBD6362229.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="KKO15_003582"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="KKO15_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBD6362230.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="KKO15_003583"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="KKO15_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBD6362231.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="KKO15_003584"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="KKO15_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBD6362232.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="KKO15_003585"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="KKO15_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD6362233.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="KKO15_003586"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="KKO15_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBD6362234.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="KKO15_003587"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="KKO15_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBD6362235.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8425
                     /locus_tag="KKO15_003588"
     CDS             7649..>8425
                     /locus_tag="KKO15_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD6362236.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVG"
BASE COUNT         1885 a         2288 c         2271 g         1981 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgctg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgtgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctggggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggt
//
LOCUS       DAECOD010000144         8225 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.144, whole genome shotgun sequence.
ACCESSION   DAECOD010000144 DAECOD010000000
VERSION     DAECOD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.144"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..305
                     /locus_tag="KKO15_003589"
     CDS             <1..305
                     /locus_tag="KKO15_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD6362237.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2205
                     /locus_tag="KKO15_003590"
     CDS             277..2205
                     /locus_tag="KKO15_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBD6362238.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2189..2392
                     /locus_tag="KKO15_003591"
     CDS             2189..2392
                     /locus_tag="KKO15_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6362239.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="KKO15_003592"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="KKO15_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="KKO15_003593"
     CDS             3972..5477
                     /locus_tag="KKO15_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBD6362240.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASDRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="KKO15_003594"
     CDS             5514..5861
                     /locus_tag="KKO15_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBD6362241.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="KKO15_003595"
     CDS             5919..6947
                     /locus_tag="KKO15_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBD6362242.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="KKO15_003596"
     CDS             6999..7385
                     /locus_tag="KKO15_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD6362243.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="KKO15_003597"
     CDS             7397..7774
                     /locus_tag="KKO15_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6362244.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..>8225
                     /locus_tag="KKO15_003598"
     CDS             7761..>8225
                     /locus_tag="KKO15_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6362245.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1903 a         1935 c         2543 g         1844 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgcc gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgct cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgcgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac ctgggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtgttttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgctggcggc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tggctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagtcctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca gacagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggcagcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacgacccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccg
//
LOCUS       DAECOD010000145         7987 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.145, whole genome shotgun sequence.
ACCESSION   DAECOD010000145 DAECOD010000000
VERSION     DAECOD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.145"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(336..411)
                     /locus_tag="KKO15_003599"
     tRNA            complement(336..411)
                     /locus_tag="KKO15_003599"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Asn,seq:gtt)
     gene            complement(512..2139)
                     /gene="yeeO"
                     /locus_tag="KKO15_003600"
                     /pseudo
     CDS             complement(512..2139)
                     /gene="yeeO"
                     /locus_tag="KKO15_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2144..2219
                     /locus_tag="KKO15_003601"
     tRNA            2144..2219
                     /locus_tag="KKO15_003601"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2177..2179,aa:Asn,seq:gtt)
     gene            complement(2257..3207)
                     /gene="cbl"
                     /locus_tag="KKO15_003602"
     CDS             complement(2257..3207)
                     /gene="cbl"
                     /locus_tag="KKO15_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBD6362246.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3309..4226)
                     /gene="nac"
                     /locus_tag="KKO15_003603"
     CDS             complement(3309..4226)
                     /gene="nac"
                     /locus_tag="KKO15_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBD6362247.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4554..4629
                     /locus_tag="KKO15_003604"
     tRNA            4554..4629
                     /locus_tag="KKO15_003604"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Asn,seq:gtt)
     gene            complement(4685..5620)
                     /gene="ldtA"
                     /locus_tag="KKO15_003605"
     CDS             complement(4685..5620)
                     /gene="ldtA"
                     /locus_tag="KKO15_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6362248.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5682..6761)
                     /gene="cobT"
                     /locus_tag="KKO15_003606"
     CDS             complement(5682..6761)
                     /gene="cobT"
                     /locus_tag="KKO15_003606"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBD6362249.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6773..7516)
                     /gene="cobS"
                     /locus_tag="KKO15_003607"
     CDS             complement(6773..7516)
                     /gene="cobS"
                     /locus_tag="KKO15_003607"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBD6362250.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7513..>7987)
                     /gene="cobU"
                     /locus_tag="KKO15_003608"
     CDS             complement(7513..>7987)
                     /gene="cobU"
                     /locus_tag="KKO15_003608"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBD6362251.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2210 a         2138 c         1727 g         1912 t
ORIGIN      
        1 atatttatta aaatggcagg tcttcttttt ctgattaccg aagacaaagt ggcaattacg
       61 tatagtttcc cgataaagac gcgacagcta cgtcccgcat aaggcatttt tctctttatc
      121 tttgtacggt acttcatgga acagaatttt tgaccttgtg aatcgtgata tctgttgggg
      181 aggggcaatt tgctcactga agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa
      241 aaaacccggc cactttttta caaagtggcc gggtaaaaat aatttttatt ttttaactgt
      301 cctgagtctc atagagatgt ctcaaaacta aaatttggct cctctgactg gactcgaacc
      361 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga
      421 acgaggcgaa tattagcgat gcccacccac aatgtcaaag cctgtttttt aaatttgaaa
      481 tcgtttgctg aaataatctg cattttgtcg tttattccga cacaactggc tttttttcac
      541 acttttgcgg ctcggatcga gggtatttcc acagccaacg tccagtaacc attcgccagt
      601 aaaacagcac ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac
      661 cccagccaag catgattccc tgcacataac cgactacaac ccggcaaccc cacatgctca
      721 acatagaaac ccacatggcg taacgggcat cacgagcacc cttaaatcca gcgggtagca
      781 cccatgaggc ggaccaaata ggcataaata aagcatttag ctaaatcaga atcacaacga
      841 cgtgtttaac ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg
      901 ttagccaggc gatggccgtt aatccaagag tggacagcca gaacacatgc cgcaactgaa
      961 tctctgcttg cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag
     1021 aaccgagcgc acttccgggt aagttgataa gagccgcaat tgagaacgcg ataaaatttc
     1081 cggcaataac gctggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg
     1141 taaataacac tgattcgaca ctcgctggaa taccaatccc catgacttcc cagataatgc
     1201 taaaattcag cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa
     1261 tcgccagcac ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccaacc
     1321 cagccccgac caggagaaaa ggccgtaaat caatatgccg ctaataataa tattaagaat
     1381 attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac gaagtgcccc
     1441 gctaccaata agagtgatgg cagcggctgg ataactgagt acggtcagct ccagataagt
     1501 caacgccagt gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc
     1561 gccaaaatga tgaattagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga
     1621 ctgccgcgtc gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa atgctacaac
     1681 gacagtagta ccaagatcga tagctgcaaa aaaagccata atgaccatat tgaagctgtc
     1741 cgccaacccc acgccggcca tcgcatcttt tcccagccaa ctgaccagaa aagtgctcag
     1801 gacccccatc aacaggacac aggcgttctc catgaagata ggaacagcaa gcggggttat
     1861 ctcgcgctag aacaacactt tgtagctctt gcgtttagag tgccagcgag tgccgtgaac
     1921 aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa agtgaaacca
     1981 catttcaaat gatgagggag aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct
     2041 gcaaatgcaa caaactgttg atagaaacgg caaacagttg ggaaatttaa aaatcgggtt
     2101 tgacaaaata tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc
     2161 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
     2221 atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc
     2281 ttcactgttt tccatcacct ggcgcttgat atcctcaact gacagtcctg cattacaaag
     2341 ttccagaaag cgccaaacat agttacgctg aagttgtcct cgcttcagtc ccaaccagac
     2401 agtattagca tcgaaaagat gccgcgtatc caggcggatt aaattctctt cctcttgttc
     2461 gccgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat
     2521 gacatcagaa tcctgtgcac ttaatacaat atctgccggc aaacctttgc gggtaaatgc
     2581 gtcatcagtg cgtgagcgcc ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc
     2641 tattgattcc agcgtcaatg gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa
     2701 actatggtgc caacaaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc
     2761 gctggcgata ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga
     2821 accctggatt agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc
     2881 tggcaagcta taacgtgcct gagtatgcgt cgttgcaata gtgagaacac cagacgtatc
     2941 gttggtaaac aggtctgcaa gccgacgaac attactggct tcattcagaa tacgttctgc
     3001 aatgaccagt aatgctttgc ccggttcagt catccccagc agtcgcttac ctcgtcgaac
     3061 aaatatttcg atgccaaatt catcctccag ttcccgaata tgacggctaa cgcctgactg
     3121 tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcctcgcg
     3181 gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat atcccgttat
     3241 tgttaaagtc taatgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga
     3301 tataacgctt agctcaccaa ttgccactgc cttttttcca tcactggaga actaatcact
     3361 gacattaaca actctttcac tgcctgtgcc tgtggtgaca agtttgctct ggcgggtaaa
     3421 tttaaggaca aagagagact cattgaagga gtggtaatgc gtgacatcca cccatttact
     3481 gcgccacata acgaacgcgc agccgattca ggtaatactg caacgcccat gccgctggca
     3541 atcgctgcgg taagcgtggc aatagactca atttcaccaa tgacttttgc cgtgagccgc
     3601 cgtagggaaa aagcctcatc aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa
     3661 aggttcattt gcgcaatagc attaacatca acgttttgcc ccgggcaatc ttgagttcct
     3721 accaggaaaa gatcttcttt cagcaaagcc tgactggata ccccagccac aggggaatgc
     3781 tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag cactgcacca
     3841 ctattttcat gaagatagat aacgacctcc gggaattcag cgcgaaccgc ctgtaataag
     3901 ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac ttgccccgat
     3961 aatgactgac caacgttatg caccgccagt tgggcctgct cacactgacg taaaatggcc
     4021 cgtgcatggg tatagagaat ttttccggcg tctgttggcg taacgccccg ttttgtgcga
     4081 atcaaaagct gttgatttaa ctcaccttcc agtatggcaa cctgctggct gagcgctggt
     4141 tgtgcgatat gcaatacttc agcagcctgg gtcaggctac caatatctac aatttttacg
     4201 aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttaagaatcg gcccaagtgc
     4261 cgccattact tacaaccagt atgcaagatg cctgccagtt ctattttggc gttgatgcgc
     4321 aagctaacca actgtcaaat aagagattat gatagattcg tcattggccc tttttatcag
     4381 cagtcgcatt cacctgccct ataaaaggag ggtatgcacc acgatggttc attacccaat
     4441 aagattgaaa gctcaccact ttgttgaaat tggcagcaaa caaacaaaaa aaatgcattt
     4501 caccctttga catcaccatg cactgccatt aatatgcgcc ccgttcacac gattcctctg
     4561 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     4621 gaggagccaa attcaaaaaa gcctgctttc gagcaggctt tttactttct aattaccaac
     4681 gctcttaaaa catctgtctt gaaccagaac taatttgcac aggcattccc gatcgatgtt
     4741 gcaacgcagc atttgcgcga tttacatcaa cttcttgccc gttgataaac gcccgcaaag
     4801 atggggttac cggcaatggc acttttcggt cagactcata ttctgcacga ttgcgcgaca
     4861 gcggctcatg aacttccagc cactttgaac catccggttc cgttgtgtat ttcactggct
     4921 gatcaataat ttgtacacga gtgcctacag gaacattatc aaacagatat ttgatatcgt
     4981 cattacgcag acgaatacag ccctgactta cacggagccc aataccaaaa ttggcattgg
     5041 taccgtggat ggcatacaac ctgccaatat aaatcgcgta cagccccatg gggttatcgg
     5101 gccccgcagg aacaaatgcg ggcaaactct cccctcgttt cgcatattcg cgccgggtgt
     5161 tcggcgttgg cgtccaggtt ggtgcttctt gtttacgttc aacggtagtc acccagttac
     5221 gcggggtttc tcgcccagcc tggccgatac caataggaaa gacttccact gtattactgt
     5281 ctggcggata gtaataaaga cgcatttcag cgacgttaac cacaatccct ttacgaacag
     5341 tggcgggcaa aatcagttgc tgcggaatgg tgagttgtga gccaggcttc ggcaaaaaaa
     5401 catcagcgcc cggattcgct tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa
     5461 aagtctccag cggctgagta ttgtgatcag gaacagttac agtaagcgac tgccccacta
     5521 aacggctacc ctctggaggt aatggataag ttaccgccag gctattttgg ctggcaaaaa
     5581 gcagagcaaa tgagcaaaga atatttacac gacgcatcat gtccctttcc taagtcgcga
     5641 atactatccg ataagtatag cttttatcag acttttcgtt tttagctgtt caaatcagaa
     5701 gtcgtattcc ccggtagaac aatattactg gcagcaagtt cgcccatgtt gttgtatatc
     5761 gcacaagcag cttcgatgat ggacatcgcc agagctgcac cactcccctc acctaaacgc
     5821 atatccatat tgagataagg ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt
     5881 tctgccgaca agtgagaagg aatgagatac ggtttgattg caggagacat ctggcaggct
     5941 gcgagcgcag cagcataaga aagaaatcca tccagcaaca cgggtaaacc gcaggaagca
     6001 gcacctaaca tcactccagc tattccgacc aaatcaaatc cacccacttt tgccaggaca
     6061 tcaacaccat cctgaggatt tggttgattc aacgtaatcg cccgacgcac aacatcaatt
     6121 ttattagcca gtttatctgt cggcaggttt gcgccaatcc caaccacttc ttcaggatcc
     6181 cggccagtga ttgtgctgac tattgccgct gccggtgtcg tgtttgccat ccccagttca
     6241 cctacaccaa acagcgtgac accgtttttc gccagctccc gcgtataaca tatgacgtcc
     6301 aaaagcaact tttcagcctg acggcgactc attgccggag ctgaagcaat attgccgcta
     6361 cctcgtgcga cacgcatgtt gataagcccg gggataggct cagcagtatc aataccaaca
     6421 tcaattacgt ggacgttagc gcccgcttgt gctgccagca cacacacgcc ggttgttcca
     6481 cgggtcatat tttcagcctg tatggctgtc acttcttttg gagaaatagc gaccccttcc
     6541 tcccagacgc cgtgatcggc acacataacc agtaccgctt ttttgcccac atgcggtatg
     6601 ccattcaccc ccggcattcc tgccagttgt atggcaagcg cctccagctt tcctaggcta
     6661 ccaacaggtt tgaataaccc gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg
     6721 atggctggga tcgtattcag taaatcggca attatttgca tgtcacgttt ctttatagca
     6781 gtgccagcag gaagaccagt tcaccaagct ctatggctgc tcccagcgta tcgcccgttt
     6841 gcccgcccag cgtacgtttg agaagctgac cgaggatgaa aatcgccacc atcgtcacca
     6901 ccatagcagc cacaccatgc atacccagca ataatacggc agcaaaaata gcggctaagc
     6961 cgagtgtgac gcaggtttgt cgcccatcaa ttttgccaat gaacacattg ccaagccctt
     7021 cttcccgcgc ataacgatga cgatacatca ataatgcggc agtcccgcgg ctgaccgcac
     7081 atgctgccgc cagcgatgca aggatcggtt cgccacgcag agccaactcg cttaacacca
     7141 gaatctttgc cagtaccaca aaaatcaatg ccagaccgcc gtgggtgcct aaacggctat
     7201 cacgcatgat ttccaacatg cgatcgcgac tacgtgcaga aaatacgcca tcacaggtat
     7261 cagcgaggcc gtccagatgg aattccccgg tcatcagcgc cagcacaagt acgctaaaca
     7321 gtgccgccag tggtgcgcca caccatgcct gcagcaccat gaagaccagc ccgctaatcg
     7381 cgccaagcaa taatccaatc aaaggaaaag taataatacc gcgagaataa tgctcgaaat
     7441 ccagtccctg ggaccagcga cgcggtacag gcagacgcgt aataaatgag agcatcgccc
     7501 aaaataattt gctcatttga tttttactcc aatacccgaa accaccagcc atacttcatt
     7561 tgccgcagcg gccaactgct gatttacccg cccggcaata tcacgaaaat gtcgtgccag
     7621 acgactctcc ggcacaatcc ccattcccac ttcgttagtc actaatacaa cctttgcggg
     7681 gcaacgttgg caggcagcaa tcaacgactg aatctcagca ttaatcgcct gttccatcgc
     7741 ctgataatcc cattcatcag ggtctttatc gccgccataa tcaaacaaca gattagtcac
     7801 cattgtggta acgcattcca gcaacacagc ctcattcggg ttaatgtctg catgaattaa
     7861 ttcatcaaga tgttgccagc gctccactgt gcgccagtgc tccgggcggc tttgccgatg
     7921 atgttctatc cgtgcagcca tctcatcatc aaggatttgc gaggtagcga tatacagaac
     7981 ctgtgaa
//
LOCUS       DAECOD010000146         7814 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.146, whole genome shotgun sequence.
ACCESSION   DAECOD010000146 DAECOD010000000
VERSION     DAECOD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.146"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..830)
                     /locus_tag="KKO15_003609"
     CDS             complement(<1..830)
                     /locus_tag="KKO15_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBD6362252.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="KKO15_003610"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="KKO15_003610"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBD6362253.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="KKO15_003611"
     CDS             complement(2479..2706)
                     /locus_tag="KKO15_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBD6362254.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KKO15_003612"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KKO15_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBD6362255.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KKO15_003613"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KKO15_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBD6362256.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KKO15_003614"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KKO15_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBD6362257.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="KKO15_003615"
     CDS             complement(5528..6511)
                     /locus_tag="KKO15_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6362258.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="KKO15_003616"
     CDS             complement(6557..7381)
                     /locus_tag="KKO15_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6362259.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KKO15_003617"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KKO15_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBD6362260.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(7740..>7814)
                     /locus_tag="KKO15_003618"
     CDS             complement(7740..>7814)
                     /locus_tag="KKO15_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6362261.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2094 a         2033 c         1830 g         1857 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc aataggtaat gactccaact
     7741 tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatgcag ctccaccgat
     7801 tttgagaacg acag
//
LOCUS       DAECOD010000147         7782 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.147, whole genome shotgun sequence.
ACCESSION   DAECOD010000147 DAECOD010000000
VERSION     DAECOD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.147"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="KKO15_003619"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="KKO15_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="KKO15_003620"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="KKO15_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBD6362262.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="KKO15_003621"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="KKO15_003621"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBD6362263.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="KKO15_003622"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="KKO15_003622"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBD6362264.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="KKO15_003623"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="KKO15_003623"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBD6362265.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1761 a         2022 c         2104 g         1895 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgacgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc cgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DAECOD010000148         7741 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.148, whole genome shotgun sequence.
ACCESSION   DAECOD010000148 DAECOD010000000
VERSION     DAECOD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.148"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="KKO15_003624"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="KKO15_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBD6362266.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMLEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="KKO15_003625"
     CDS             1102..2562
                     /locus_tag="KKO15_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD6362267.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KKO15_003626"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KKO15_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBD6362268.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KKO15_003627"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KKO15_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBD6362269.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDADAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KKO15_003628"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KKO15_003628"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBD6362270.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="KKO15_003629"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="KKO15_003629"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBD6362271.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="KKO15_003630"
     CDS             complement(6727..7245)
                     /locus_tag="KKO15_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBD6362272.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7741
                     /gene="ydeI"
                     /locus_tag="KKO15_003631"
     CDS             7521..>7741
                     /gene="ydeI"
                     /locus_tag="KKO15_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBD6362273.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISH"
BASE COUNT         1939 a         1810 c         1956 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcta gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcggc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 a
//
LOCUS       DAECOD010000149         7536 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.149, whole genome shotgun sequence.
ACCESSION   DAECOD010000149 DAECOD010000000
VERSION     DAECOD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.149"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KKO15_003632"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KKO15_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KKO15_003633"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KKO15_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBD6362274.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KKO15_003634"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KKO15_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KKO15_003635"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KKO15_003635"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD6362275.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KKO15_003636"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KKO15_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBD6362276.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KKO15_003637"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KKO15_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBD6362277.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KKO15_003638"
     CDS             complement(6510..7202)
                     /locus_tag="KKO15_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBD6362278.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1825 c         1984 g         1809 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatggacgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tctcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcg
//
LOCUS       DAECOD010000150         7199 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.150, whole genome shotgun sequence.
ACCESSION   DAECOD010000150 DAECOD010000000
VERSION     DAECOD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.150"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(274..361)
                     /locus_tag="KKO15_003639"
     tRNA            complement(274..361)
                     /locus_tag="KKO15_003639"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Ser,seq:gga)
     gene            596..1534
                     /gene="ghrA"
                     /locus_tag="KKO15_003640"
                     /pseudo
     CDS             596..1534
                     /gene="ghrA"
                     /locus_tag="KKO15_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            1589..2326
                     /gene="ycdX"
                     /locus_tag="KKO15_003641"
     CDS             1589..2326
                     /gene="ycdX"
                     /locus_tag="KKO15_003641"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBD6362279.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2350..2904
                     /gene="ycdY"
                     /locus_tag="KKO15_003642"
     CDS             2350..2904
                     /gene="ycdY"
                     /locus_tag="KKO15_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBD6362280.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3006..3497
                     /locus_tag="KKO15_003643"
     CDS             3006..3497
                     /locus_tag="KKO15_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBD6362281.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3630..4336)
                     /locus_tag="KKO15_003644"
                     /pseudo
     CDS             complement(3630..4336)
                     /locus_tag="KKO15_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(4363..4779)
                     /gene="csgF"
                     /locus_tag="KKO15_003645"
     CDS             complement(4363..4779)
                     /gene="csgF"
                     /locus_tag="KKO15_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBD6362282.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(4804..5193)
                     /gene="csgE"
                     /locus_tag="KKO15_003646"
     CDS             complement(4804..5193)
                     /gene="csgE"
                     /locus_tag="KKO15_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBD6362283.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(5198..5848)
                     /gene="csgD"
                     /locus_tag="KKO15_003647"
     CDS             complement(5198..5848)
                     /gene="csgD"
                     /locus_tag="KKO15_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD6362284.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            6600..7055
                     /gene="csgB"
                     /locus_tag="KKO15_003648"
     CDS             6600..7055
                     /gene="csgB"
                     /locus_tag="KKO15_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBD6362285.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            7096..>7199
                     /locus_tag="KKO15_003649"
     CDS             7096..>7199
                     /locus_tag="KKO15_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBD6362286.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         1912 a         1567 c         1728 g         1992 t
ORIGIN      
        1 ttccattgtc gattcctctg gcataacgat tttatgatgg catgtgacat gtatttccgt
       61 tgggggcatt ttaataagtg aggaagttac aggaagtgac cagataacgc atatatgttc
      121 tgtactatct tacgcatttt gattgttgac tgagtaacca gacagttgat gtgcacaatt
      181 tcccctcgcc ctaacagacg tgggcggggg cacccggtgt gtacaatata cgaaaaaaaa
      241 gcccgtactt tcgtacgcgc tcttctttaa atatggcggt gaggggggga ttcgaacccc
      301 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
      361 caaattgtcg ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg
      421 tcaacaaact ttttgaaaaa atcgcgcgtt tattcaaaca ttcaatcaat gtgtggtttt
      481 aataagcgaa atctgctttt tttgccaccg accacggatt tgttatgctg gtggcctttg
      541 tagatcataa cgataagtgc gaataaattt cgcacaacgc ttttcgggag tcagtatgga
      601 tatcatcttt tatcacccaa cgttcgatac ccaatggtgg attgaggcac tgcgcaaagc
      661 tattcctcag gcaagagtca gggcatggaa aagcggagat aatgactctg ctgattatgt
      721 tttagtctgg catcctcctg ttgaaatgct ggcagggcgc gatcttaaag cggtgttcgc
      781 actcggggcc ggtgttgatt ctattttgag caagctacag gcacaccctg aaatgctgaa
      841 cccttctgtt ccactttttc gcctggaaga taccggtatg ggcgagcaaa tgcaggaata
      901 tgctgtcagt caggtgctgc attggtttcg acgttttgac gattatcgca tccagcaaaa
      961 tagttcgcat tggcaaccgc tgcctgaata tcatcgggaa gattttacca tcggcatttt
     1021 gggcgcaggc gtactgggct gtaaagttgc tcagagtctg caaacctagc gctttccgct
     1081 gcgttgctgg agtcgaaccc gtaaatcgtg gcctggcgtg caaagctttg ccggacggga
     1141 agaactgtct gcatttctga gccaatgtcg ggtattgatt aatttgttac cgaatacccc
     1201 tgaaaccgtc ggcattatta atcaacaatt actcgaaaaa ttacctgatg gcgcgtatct
     1261 cctcaacctg gcgcgtggtg ttcatgttgt ggaagatgac ctgctcgcgg cgctggatag
     1321 cgggaaagtt aaaggcgcaa tgctggatgt ttttaatcgt gaacccttac cgcctgaaag
     1381 tccgctctgg caacatccac gcgtgacgat aacaccacat gtcgccgcga ttacccgtcc
     1441 cgctgaagct gtggagtaca tttctcgcac tattgcccag ctcgaaaaag gggagagggt
     1501 ctgcgggcaa gtcgaccgcg cacgtggcta ctaataaagc atcaggattc ctgctatcct
     1561 tggcgggaat tgaatacagg agagagttat gtatcccgtc gaccttcata tgcataccgt
     1621 tgccagcaca catgcatata gcacattgag tgattacatt gcccaggcca aacaaaaggg
     1681 cattaaactt tttgcgatca ccgatcatgg cccggatatg gaagatgcgc cgcatcactg
     1741 gcacttcatt aacatgcgta tctggccgcg agtggttgat ggggtaggga tcctgcgcgg
     1801 cattgaagct aacattaaaa atgttgatgg tgaaattgac tgcagcggta aaatgtttga
     1861 ctcgctggat ctgattattg ccggttttca tgagccggtt tttgcgccac atgacaaagc
     1921 gaccaataca caagcgatga tctccactat cgccagcggc aatgtgcata taataagcca
     1981 tcccggaaat ccgaaatatg aaatagatgt taaagccgtt gctgaagcag ccgcgaaaca
     2041 tcaggtggcg ctggaaatca ataattcctc atttttacac tcacgtaagg gcagtgaaga
     2101 caactgtcgt gcggtagctg cagcggtacg tgatgctgga ggttgggtgg cattaggctc
     2161 ggattctcac accgcgttta ccatggggga atttgaagag tgtcttaaaa tcctcgacgc
     2221 ggtagatttt ccgctagagc gcattttgaa tgtttctccg cgccgcttac tgaacttcct
     2281 tgaatctcgc ggtatggcac cgattgcgga atttgcagac ctttaattac tagtaatgga
     2341 aatatattaa tgaacgagtt ttctatcctc tgtcgtgtgc tgggttcgct ctattaccgc
     2401 caaccgcaag atcctttact ggtgccgctg tttactctga ttcgtgaggg gaaactggct
     2461 gcgaactggc cactggagca ggatgagtta ctgacgcgtt tacagaaaag ctgtgatatg
     2521 acgcaagtct ctgccgatta caatgcgttg tttatcggcg atgaatgtgc cgtgccgcca
     2581 tatcgtagcg catgggttga ggacgcaacg gaagcggaag tgcgcgcttt tctttccgaa
     2641 cgagggatgc cactggcgga tacgccagcc gatcacatcg gcacattgct gcttgcggct
     2701 tcctggctgg aagatcagtc aacggaagat gagagcgaag cactggaaac actgttcagt
     2761 gagtatctgt taccctggtg tggtgcgttc ctcggcaaag tggaggccca tgcaaccacg
     2821 cctttctggc gcaccatggc accgctaacc cgtgatgcca ttagtgcaat gtgggacgag
     2881 ctggaagaag attctgaaga gtaaatgtga tcatcgtcac tcataactgc aacagataat
     2941 ttgttgttgc ataaaatgtg tgctcgatct cattcatggc cgcgttttct gctatcatgc
     3001 gcggcatgaa catacttctc tctattgcaa tcacaacagg cattctctcc ggtatctggg
     3061 gatgggtggc tgtttccctg ggcttactaa gctgggcggg cttcctaggc tgtacggcct
     3121 actttgcttg cccgcaaggt gggctgaaag ggctggcgat ctccgctgca accctgctta
     3181 gtggcgtggt gtgggcgatg gtcattattt acggtagtgc actggcacca catctggaaa
     3241 ttctcggtta tgtcataacc ggtattgtcg cgtttctgat gtgtattcag gccaaacagc
     3301 tgctgctttc atttgttccg gggacgttta taggcgcatg cgcgacgttt gccgggcagg
     3361 gtgactggaa actggtgtta ccttcgctgg cgcttgggct ggtatttggt tacgcaatga
     3421 aaaacagcgg cctgtggctg gcggcgcgta gtgcaaagac ggctcaccgt gagcaggaaa
     3481 tcaaaaataa agcgtgaggg gcactcacgc tttcgcttaa acagtaaatg ccggattatt
     3541 aattccggca tttttatctg tcaggattcc ggtggaaccg acatatggcg gtatttcacc
     3601 agaatgtcat tctgccgttc tgctttattt tacaaatccc acagaccacg gtcgatacca
     3661 tcattaatca ggaaaatgac ccctgtttcg atagccgaca tcagacacag cataacgggt
     3721 tcgtttgagg tgtaacccac ttccccttcc agcaagcgct ggtagtcaat aaagcggaaa
     3781 accccggcct gaacttcata ggaaagtatc gtcttactgg tgttcaccga ggagaggatc
     3841 tcaccggtac tcacattgac gacacgcagg ttcacggcaa tctgatcgag ctggtattgc
     3901 gtgtctgcac cgatcccaaa atatcttgcc ccaacaccgc ccgatttaac gttgctttca
     3961 taaccgataa tcgaaccttc aaccataata tttgccgccg ttaaagattg cagcgggatt
     4021 cggttattaa tggcaaccgt gccgttttct tgtgccgcac gaataacctt gcgctcgtta
     4081 agcaggtttt gtaagccctg gcgctttgcg gaacagcagt ggagaagtta cttgccgggt
     4141 agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac cgaaacaaag atcttacccg
     4201 tcggcgctgg cagatgggtc aaatctttgt agctctgagc acgaggcatt aatgtcggtc
     4261 tggcggcttc tttaggcggg gcggttaagc atccgctcag taacatgacg gcaaccaaaa
     4321 gaaataagcg ctgcatgatt attttcctta tgaagctggg gcttaaaaat cggttgagtt
     4381 attttgtaaa cccgaaacct ggatggtcga ggtttgtccg gttttacgat ctgtcacgtt
     4441 caactgcaat tgaccatcgc ggttagcaat atcaacaata taatcgttgg tcaccatgcg
     4501 gcccggttta ccggtattaa tattcgacag tagcccacct aaaatttgtg actggatggc
     4561 ctgagtaaag ttatctaacg ctgagggtgt ttcaatacca aagtcatcgt tatagctcgg
     4621 atctttataa gagttttggg cctgagcgct atttaataaa aaagcgccat tatttgggtt
     4681 accaccaaag tttggattac ggaactggaa agtcatggtt ccagcccaac ttaatggcga
     4741 aataagcatg agtagaacta ctgcatgttt gacacgcatt gcagcctccg aacaattttt
     4801 tatttagaat tcatcatgcg ccaaatcgtc cgtacttaat aacgcctgat ttatctggcg
     4861 acgatttagt gcttcttcag tttgaatcag tgcaaagacg acagttttct cgaagtctct
     4921 tttcaacggg aataaaaaag tctggaaaat aacatcctga ttgaccgtta ttgtgatcca
     4981 gcttccccat cgtgcactgg gcctttcatt aatcgttaag ttacccgtat agtcactttc
     5041 ccatttatca ctaaaggctc ggtaaaaatc atggccaata gatgaaacag tatggtcagt
     5101 taacaatccc gggacttcta cctcaacggc gtgaagattc cctgcggcga acagaaattc
     5161 tgccgccaca atccagcgta aataacgttt catggcttta tcgcctgagg ttatcgtttg
     5221 cccaggaaac cgcttgtgtc cggtttttta cggctatctt cttgaaaaga ttataaagat
     5281 gcgttttaac cgtattttcg ctgatgaaca acgaacgagc gatctcgtta ttagacgcgc
     5341 cgatacgcag cttattcagg atctcttttt cccgatgagt aaggagggct gattccgtgc
     5401 tgttataacg atagttacca gaatgtgtaa tcaggtaact ggcaagcttt tgcgtaaagt
     5461 agcattcgcc acgcaggacg ccttgcaacc cattgacaac acgttcttga tcctccatgg
     5521 cataaaaaac gccgttgata tgaggccagt tttcaatgtc gcggtacggg taatcttcag
     5581 gcgtatttag caacaatatt ttgatattgt tgtttttcct gctcaaagta tcctgccaat
     5641 aatggataag ctttttatcc gcttccatca tatccagaag aataatagag cctgaagaga
     5701 tatcgtccag agaacgttga atattatgta attttcctgt aattgctagc gattgtttaa
     5761 ggtgctgcaa gagagctgtc gcctgcaaag aaggtttagt gatcaacaat aatgtatgac
     5821 catgaatact atggacttca ttaaacatga tgaaacccca cttttttatt gatcgcacac
     5881 ctgacagctg cctctaaaat agaagcacca gaagtactga cagatgttgc actgctgtgt
     5941 gtagtaataa atcagcccta aatgggtaaa atataaaact aatggattac atctgatttc
     6001 aatctagcca ttacaaatct taaatcaagt gttaaacatg taacaaaatg taactcatta
     6061 tattaaaatg ttaacattaa ggttttatta agtttagaaa tgatagaaaa gttgtacatt
     6121 tggtttttat tgcacaattt taaaaaatca tacaaatggt gctaacttac taataataca
     6181 tataaaaata tttcggtgta gtcctttcgt catgtaaaac gtttttgttt tttctccaca
     6241 cctccgtgga caatttttta ctgcaaaaag acgaggtttg tcacggcttg tgcgcaagac
     6301 atatcgcagc aatcagcgac gggcaagaag aatgactgtc tggtgatttt tgatagcgga
     6361 aaacggagat ttaagagaaa acaaaatatt ttttgcgtag ataacagcgt atttacgcgg
     6421 gttttaatac tttggtatga acaaaaaaag aaaaatacaa cgcgcgggtg agttattaaa
     6481 aatatttccg cagacatact ttccatcgta acgcagcgtt aacaaaatac aggttgcgtt
     6541 aacaaccaag ttgaaatgat ttaatttctt aaatgtacga ccaggtccag ggtgacaaca
     6601 tgaaaaacaa attgttattt atgatgttaa caatactggg tgcgcctggg attgcagccg
     6661 cagcaggtta tgatttagct aattcagaat ataacttcgc ggtaaatgaa ttgagtaagt
     6721 cttcatttaa tcaggcagcc ataattggtc aagctgggac taataatagt gctcagttac
     6781 ggcagggagg ctcaaaactt ttggcggttg ttgcgcaaga aggtagtagc aatcgggcaa
     6841 agattgacca gacaggagat tataaccttg catatattga tcaggcgggc agtgccaacg
     6901 atgccagtat ttcgcaaggt gcttatggta atactgcgat gattatccag aaaggttctg
     6961 gtaataaagc aaatattaca cagtatggta ctcaaaaaac ggcaattgta gtgcagagac
     7021 agtcgcaaat ggcaattcgc gtgacacaac gttaattacc attcgacttt taaatcaatc
     7081 cgatgggggt tttacatgaa acttttaaaa gtagcagcaa ttgcagcaat cgtattctcc
     7141 ggtagcgctc tggcaggtgt tgttcctcag tacggcggcg gcggtaacca cggtggtgg
//
LOCUS       DAECOD010000151         7184 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.151, whole genome shotgun sequence.
ACCESSION   DAECOD010000151 DAECOD010000000
VERSION     DAECOD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.151"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(296..1198)
                     /locus_tag="KKO15_003650"
     CDS             complement(296..1198)
                     /locus_tag="KKO15_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBD6362287.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KKO15_003651"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KKO15_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBD6362288.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KKO15_003652"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KKO15_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBD6362289.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KKO15_003653"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KKO15_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBD6362290.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KKO15_003654"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KKO15_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBD6362291.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKELLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KKO15_003655"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KKO15_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="KKO15_003656"
     CDS             7090..>7184
                     /locus_tag="KKO15_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBD6362292.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1673 a         1813 c         1877 g         1821 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacacgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgag catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgaaccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca ttaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agttgctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAECOD010000152         7158 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.152, whole genome shotgun sequence.
ACCESSION   DAECOD010000152 DAECOD010000000
VERSION     DAECOD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.152"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            18..1076
                     /locus_tag="KKO15_003657"
     CDS             18..1076
                     /locus_tag="KKO15_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD6362293.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="KKO15_003658"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="KKO15_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBD6362294.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGTKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="KKO15_003659"
     CDS             complement(1623..1727)
                     /locus_tag="KKO15_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362295.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="KKO15_003660"
     CDS             1897..2343
                     /locus_tag="KKO15_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBD6362296.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="KKO15_003661"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="KKO15_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="KKO15_003662"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="KKO15_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="KKO15_003663"
     CDS             4450..4797
                     /locus_tag="KKO15_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBD6362297.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="KKO15_003664"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="KKO15_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBD6362298.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="KKO15_003665"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="KKO15_003665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBD6362299.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIIFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="KKO15_003666"
     CDS             6314..6412
                     /locus_tag="KKO15_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBD6362300.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="KKO15_003667"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="KKO15_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBD6362301.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6841)
                     /locus_tag="KKO15_003668"
                     /pseudo
     CDS             complement(6650..6841)
                     /locus_tag="KKO15_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7021..>7158)
                     /locus_tag="KKO15_003669"
     CDS             complement(7021..>7158)
                     /locus_tag="KKO15_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6362302.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
BASE COUNT         1731 a         1741 c         1742 g         1944 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtac taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc agtcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgctagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattattttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgcgaaagcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg cggcgaatac
     6961 tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt acccaatggg
     7021 ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt ccatatccaa
     7081 atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt tacgcatagt
     7141 gtcaggcttt aagcctgc
//
LOCUS       DAECOD010000153         6795 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.153, whole genome shotgun sequence.
ACCESSION   DAECOD010000153 DAECOD010000000
VERSION     DAECOD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.153"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            240..2672
                     /locus_tag="KKO15_003670"
     CDS             240..2672
                     /locus_tag="KKO15_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBD6362303.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="KKO15_003671"
     CDS             2739..4208
                     /locus_tag="KKO15_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBD6362304.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="KKO15_003672"
     CDS             4208..5869
                     /locus_tag="KKO15_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBD6362305.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="KKO15_003673"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="KKO15_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBD6362306.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="KKO15_003674"
     CDS             complement(6478..>6795)
                     /locus_tag="KKO15_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362307.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1611 c         1554 g         1627 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagt caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAECOD010000154         6710 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.154, whole genome shotgun sequence.
ACCESSION   DAECOD010000154 DAECOD010000000
VERSION     DAECOD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.154"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..357)
                     /locus_tag="KKO15_003675"
     CDS             complement(<1..357)
                     /locus_tag="KKO15_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362308.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALY"
     gene            complement(632..1996)
                     /gene="tdcG"
                     /locus_tag="KKO15_003676"
     CDS             complement(632..1996)
                     /gene="tdcG"
                     /locus_tag="KKO15_003676"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD6362309.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(2068..2457)
                     /locus_tag="KKO15_003677"
     CDS             complement(2068..2457)
                     /locus_tag="KKO15_003677"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBD6362310.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2471..4660)
                     /gene="tdcE"
                     /locus_tag="KKO15_003678"
     CDS             complement(2471..4660)
                     /gene="tdcE"
                     /locus_tag="KKO15_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBD6362311.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIACCVSP
                     MVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLD
                     HFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSA
                     IKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRN
                     AVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKL
                     PFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNR
                     EMLLDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4694..5902)
                     /gene="tdcD"
                     /locus_tag="KKO15_003679"
     CDS             complement(4694..5902)
                     /gene="tdcD"
                     /locus_tag="KKO15_003679"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBD6362312.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5928..>6710)
                     /locus_tag="KKO15_003680"
     CDS             complement(5928..>6710)
                     /locus_tag="KKO15_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD6362313.1"
                     /translation="PYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIMVFSFNFSPI
                     VSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLFTLSPANMAE
                     AKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGHYLGTLEGLN
                     GLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAMGAPIIASLL
                     CLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
BASE COUNT         1689 a         1809 c         1604 g         1608 t
ORIGIN      
        1 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
       61 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
      121 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
      181 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
      241 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
      301 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
      361 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
      421 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
      481 aaaatcttca caatcaagac tttaaaataa cggagatgga taagaatttt ctactaaatt
      541 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
      601 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
      661 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
      721 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
      781 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
      841 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
      901 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgctgcc attgaacagg
      961 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
     1021 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
     1081 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
     1141 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
     1201 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
     1261 gacgacgcag cgctacggca cgacacggca cattgagcgg accaggcagc acgccttcag
     1321 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     1381 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     1441 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     1501 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     1561 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
     1621 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     1681 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     1741 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
     1801 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
     1861 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     1921 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     1981 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     2041 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     2101 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
     2161 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     2221 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     2281 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     2341 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     2401 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     2461 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
     2521 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     2581 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
     2641 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccaacaaggt
     2701 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     2761 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     2821 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     2881 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     2941 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     3001 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     3061 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     3121 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     3181 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     3241 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     3301 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     3361 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     3421 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     3481 ccattgggct gacgcagcag gcaatcggta attcttccga ccaaagaatg gtcaggttag
     3541 gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca
     3601 ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag attgggtcgc
     3661 cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg atcttcatga
     3721 tgaagtgatc gatcagttcc tgtgcctgct gctcattgag gacgccagct ttaaagtcgc
     3781 gctcaatgta gatatccagg aacgatgccg tgcgccccag cgacatcgcg ccgccatttt
     3841 gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc tgtgcattct
     3901 gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc tgcaacagcg
     3961 catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga tcctcgcctt
     4021 tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgcacc agataactga
     4081 tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacgacca tagccatctg
     4141 gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag taaacatcaa
     4201 atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat tcactgtcca
     4261 tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg aacgggtgta
     4321 gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc ggctggttaa
     4381 tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg
     4441 cgtgggttgc gttttcgata cggatgcctt ccattacctt ttcccacaat tccgtggtgg
     4501 caggcgtcgc ttcggcgaga aaagattcat cgccttcata cggtgtatag ttatgttgaa
     4561 taaaatcgcg gacattaatt tcgcttttcc agtccgtacc tttaaagcca agccatgcgt
     4621 cagcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat ctctctacaa
     4681 tacttcaact aaattatgca aattctgcgg gcgcgttaac tttacctaaa tgaatggcat
     4741 ccaaagcaat cattttttct tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt
     4801 cactggaaac aattcgctcg ccaaaagagt tagatcgatt attcatttct gtatcaatct
     4861 ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaaactt gaattctctc
     4921 ctattccgcc ggtgaatata attccatcca gccgacgtaa tgaagctgca tgtccggcaa
     4981 tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt
     5041 catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata cctaataatc
     5101 ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt tggctggcga
     5161 cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc aagccttcca
     5221 gcggcgtcat tcccattgag gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg
     5281 cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga ttcagcagcg
     5341 aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac
     5401 ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga gccatcgtct
     5461 ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat tgctgcgccg
     5521 attcaatacc acttaaattt gcgtaattat gcaggggtgc cagtggagaa acgcgacgga
     5581 tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg ccgccgtgag
     5641 cgatgcggtg gccaattaag gccacgctgt catttaaatt ccgtttttcc agttcaaatg
     5701 caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc tctcccccat
     5761 ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac attaatactt
     5821 cacaatcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag ttaataacca
     5881 aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta aaacagtttg
     5941 tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt atccagacga
     6001 ccacggtatt tcgccagaga cggcgcttta cggatagcat acatcggcaa caggcacagc
     6061 agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt cgggttggcg
     6121 taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgagagtatt cagtttaccc
     6181 agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc attcaagcct
     6241 tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag tgcgatgatg
     6301 gaagccgcat attccagtgt aatcgcgaac gttgttttcg taccggtcat ggacgcaaag
     6361 tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc catgttggcc
     6421 ggagacagag taaacaggca gctaaaggca aagaacatca ccactgcaac catcagcatg
     6481 ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc gaagtctttc
     6541 tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa gttaaaggag
     6601 aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg accggttaac
     6661 gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
//
LOCUS       DAECOD010000155         6407 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.155, whole genome shotgun sequence.
ACCESSION   DAECOD010000155 DAECOD010000000
VERSION     DAECOD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.155"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KKO15_003681"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KKO15_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBD6362314.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="KKO15_003682"
     CDS             complement(1433..1759)
                     /locus_tag="KKO15_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBD6362315.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="KKO15_003683"
     CDS             1894..2235
                     /locus_tag="KKO15_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBD6362316.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="KKO15_003684"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="KKO15_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="KKO15_003685"
     CDS             complement(2833..3543)
                     /locus_tag="KKO15_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBD6362317.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="KKO15_003686"
     CDS             3651..3956
                     /locus_tag="KKO15_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBD6362318.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="KKO15_003687"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="KKO15_003687"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="KKO15_003688"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="KKO15_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1709 a         1628 c         1499 g         1571 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtacac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatgttata ttattatatt
     6001 aacaacaggt ccagaccatt ggtccagacc atagttagtt aatcacagtg atagcccacc
     6061 acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct tacctccctc
     6121 ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag gagctacagg
     6181 aggcgggacc gaagccccac cttgagtccc accaccacct gtcaggccct tggcgatgga
     6241 cctgaagcct ttactaatgc tcttacccat tggtcttacg cttcggtctt ggtgaacttc
     6301 aggcgtttga ctacaggcag gacggcagta gctgagccag tgcccatctt gacggtcgta
     6361 gcagcttcag cgacaacgaa cactttaccg aggatttcca agtcgtg
//
LOCUS       DAECOD010000156         6375 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.156, whole genome shotgun sequence.
ACCESSION   DAECOD010000156 DAECOD010000000
VERSION     DAECOD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.156"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="KKO15_003689"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="KKO15_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="KKO15_003690"
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="KKO15_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HBD6362319.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="KKO15_003691"
     CDS             complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="KKO15_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBD6362320.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="KKO15_003692"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="KKO15_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBD6362321.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="KKO15_003693"
     CDS             complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="KKO15_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBD6362322.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5702..>6375)
                     /locus_tag="KKO15_003694"
     CDS             complement(5702..>6375)
                     /locus_tag="KKO15_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBD6362323.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1532 a         1680 c         1536 g         1627 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggagtgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggacgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtggacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttatatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     5641 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     5701 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     5761 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
     5821 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
     5881 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     5941 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     6001 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
     6061 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     6121 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     6181 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
     6241 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     6301 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     6361 cgttaatgaa gatcc
//
LOCUS       DAECOD010000157         6321 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.157, whole genome shotgun sequence.
ACCESSION   DAECOD010000157 DAECOD010000000
VERSION     DAECOD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.157"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..378
                     /locus_tag="KKO15_003695"
     CDS             <1..378
                     /locus_tag="KKO15_003695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362324.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KKO15_003696"
     CDS             complement(454..918)
                     /locus_tag="KKO15_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD6362325.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KKO15_003697"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KKO15_003697"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBD6362326.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KKO15_003698"
     CDS             complement(1787..2476)
                     /locus_tag="KKO15_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBD6362327.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KKO15_003699"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KKO15_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBD6362328.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KKO15_003700"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KKO15_003700"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBD6362329.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KKO15_003701"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KKO15_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBD6362330.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAECOD010000158         6270 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.158, whole genome shotgun sequence.
ACCESSION   DAECOD010000158 DAECOD010000000
VERSION     DAECOD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.158"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..666)
                     /locus_tag="KKO15_003702"
     CDS             complement(<1..666)
                     /locus_tag="KKO15_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBD6362331.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="KKO15_003703"
     CDS             complement(656..2413)
                     /locus_tag="KKO15_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBD6362332.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFYKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="KKO15_003704"
     CDS             complement(2429..3313)
                     /locus_tag="KKO15_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD6362333.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="KKO15_003705"
     CDS             complement(3324..3929)
                     /locus_tag="KKO15_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD6362334.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(4100..>6270)
                     /locus_tag="KKO15_003706"
     CDS             complement(4100..>6270)
                     /locus_tag="KKO15_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBD6362335.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1567 c         1317 g         1550 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttataaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aagcagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgtttat
//
LOCUS       DAECOD010000159         6254 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.159, whole genome shotgun sequence.
ACCESSION   DAECOD010000159 DAECOD010000000
VERSION     DAECOD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.159"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="KKO15_003707"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="KKO15_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBD6362336.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="KKO15_003708"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="KKO15_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBD6362337.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KKO15_003709"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KKO15_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="KKO15_003710"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="KKO15_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1456 c         1476 g         1663 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgaggac
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gaggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DAECOD010000160         6175 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.160, whole genome shotgun sequence.
ACCESSION   DAECOD010000160 DAECOD010000000
VERSION     DAECOD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.160"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KKO15_003711"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KKO15_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBD6362338.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KKO15_003712"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KKO15_003712"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBD6362339.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KKO15_003713"
     CDS             complement(3736..4092)
                     /locus_tag="KKO15_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD6362340.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KKO15_003714"
     CDS             complement(4096..4512)
                     /locus_tag="KKO15_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBD6362341.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KKO15_003715"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KKO15_003715"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBD6362342.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KKO15_003716"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KKO15_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1533 a         1550 c         1555 g         1537 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttg
//
LOCUS       DAECOD010000161         6113 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.161, whole genome shotgun sequence.
ACCESSION   DAECOD010000161 DAECOD010000000
VERSION     DAECOD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.161"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            437..1465
                     /gene="torT"
                     /locus_tag="KKO15_003717"
     CDS             437..1465
                     /gene="torT"
                     /locus_tag="KKO15_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBD6362343.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1438..2130)
                     /gene="torR"
                     /locus_tag="KKO15_003718"
     CDS             complement(1438..2130)
                     /gene="torR"
                     /locus_tag="KKO15_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBD6362344.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2217..3389
                     /gene="torC"
                     /locus_tag="KKO15_003719"
     CDS             2217..3389
                     /gene="torC"
                     /locus_tag="KKO15_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBD6362345.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3389..5929
                     /gene="torA"
                     /locus_tag="KKO15_003720"
     CDS             3389..5929
                     /gene="torA"
                     /locus_tag="KKO15_003720"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBD6362346.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5926..>6113
                     /gene="torD"
                     /locus_tag="KKO15_003721"
     CDS             5926..>6113
                     /gene="torD"
                     /locus_tag="KKO15_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD6362347.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1490 a         1527 c         1689 g         1407 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgtccat
       61 ttcggtccca gttaggacgc tttctcatca agccagtccg cccaccactg catcatctcc
      121 ctacgcttat cgagatactg agcatggttg taaattcaat taacccagtg cgttgacaaa
      181 aaattagcgc aagaagacaa aaatcacctt gcgctaatgc tctgttacag gtcactaata
      241 ccatctaagt ggttgattca tagtgactgg atatgttgtg ttttgtcgcg tttttcagtc
      301 tattatttaa tcgttaagta atctaatgct atgattttta aattatttat gtgattttgt
      361 catcctttag gtgaaaaagg ttgagtcgca aagtggaatg catctagcat aaagccttat
      421 tattgatgag gctatcatgc gcgtactgct atttttactt ctttcccttt tcatgttgtc
      481 ggcattttcg gctgataacc tgttgcgctg gcatgatgcg cagcatttca cggtgcaagc
      541 ctctatgccg cttaaagcca aacgcgtatg gaaactgtgc gcgctttatc ccagcctgaa
      601 agattcatac tggttatcgt tgaactatgg tatgcaggag gctgctcgcc gctacggtgt
      661 ggatttaaaa gtgctggagg caggcggcta cagccagttg gctacccagc aagcacaaat
      721 cgaccagtgt aaacagtggg gcgcagaggc cattttgctc ggtagtagca cgacgtcatt
      781 tcccgacctg caaaagcagg tagcaaatct gccggtgatc gaactggtaa atgctcttga
      841 tgatccccag gtgaaaagcc gcgttggtgt accctggttt cagatgggct atcaacctgg
      901 gcgatatttg gtgcaatggg cgcacggtaa accactgaac gtgctgctga tgcccggacc
      961 cgataacgcc gggggcagta aggagatggt cgagggtttt cgcgcagcca ttgccggaag
     1021 cccggtgcgt attgttgata ttgcgcttgg tgataacgat attgaaatcc agcgcaacct
     1081 gttgcaggag atgctggaac gccacccaga aatcgacgtc gttgccggaa cggccattgc
     1141 ggcagaggcg gcaatgggtg aagggcgtaa cctgaaaacg ccgcttaccg tggtgtcgtt
     1201 ttatctttca catcaggtgt atcgcgggct gaagcgggga agagtgatta tggctgccag
     1261 cgatcaaatg gtctggcagg gggaactggc agttgagcag gccatcaggc aattacaggg
     1321 gcaatcggtg tctgataatg tcagcccacc gattttagtt ctgacgccga aaaatgccga
     1381 ccgagaacat atccgccgct cgctgtcacc ggggggattt cgtccggtct atttttatca
     1441 gcacacatca gcggctaaga aataaccttc accatgttgc gtcaccagta aatccgcgct
     1501 gagtttatga cgtaaacgac gaattaacac atcgacggtg cgcaggtcag ggttttccac
     1561 ccgacgcgca gaaagcatac gcagcagacg ctcacggctg agaatttcgc ccggattagt
     1621 cacaaatgcc accaacattt catactctgc gcgggtcagt ttaatcggct cgccatcccg
     1681 ctccagcgta tggcgcgaca cattcaggca ataacccgca aagcgatagc agtagtcctg
     1741 agtgtgtggt tgagcttgtc gcgcgaggtc gattcgccag agcagatttt tcacccgtac
     1801 taccagttcg cgcagttcca gcggtttggt gacgtaatcg tctgcgccca tttccagccc
     1861 aacaatacgg tcaatccgat cgctgcgtcc ggtaaccaga ataatcccca ccgttgagcg
     1921 ttctcgcagg gcgcgggtta acatcaggcc attttcatcg ggtaagttga tatccagcag
     1981 aattaaatct accggctgat tctgcataat ttcccgtagc ccggcaccgc tcgccgtaac
     2041 ggaaacggta tacccctcct gagtgaagta ggattgtaat cgcgcctggg taaccggctt
     2101 atcttcaaca ataacaatgt gatgtggcat cagagggttt tactcattct gttcatatct
     2161 gttcatattt gctcattaag atcgcttcac taaaccataa ttctacaggg gttattatgc
     2221 ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg
     2281 caattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca
     2341 ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata
     2401 aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat gactgtcata
     2461 tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaac gacatttacc
     2521 agacctttat tgcccactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac
     2581 ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgt cgctcctgcc
     2641 ataactacga tgcgatggat catgccaggc agcatcctga agcggcacgt cagatgaagg
     2701 tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac
     2761 cggatatgag tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca
     2821 gtggtgacac gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag
     2881 aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact
     2941 ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc
     3001 aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga
     3061 aaacgctgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca
     3121 ccgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg gcctatgcgg
     3181 attcgttgta caacggcacc tgtaaccagt gccacggcgc accggaaatc gcccactttg
     3241 acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagt ctcgataaac
     3301 gtgaagaacg cactttgttg aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg
     3361 ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc aggcatcacg
     3421 tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt
     3481 gttaacgccg cgccgtgcaa ctgcggcgca agcggcgact gaggctgtca tctcgaaaga
     3541 gggcattctt accgggtcgc actggggggc tatccgcgcg atggtgaagg atggtcgctt
     3601 tgtggcggca aaaccgttcg aactggataa atatccgtcg aaaatgatcg ccggattgcc
     3661 ggatcacgta cacaacgcgg cacgtattcg ttatccgatg gtacgcgtgg actggctgcg
     3721 taagcgccat ctgagcgaca cctcccagcg cggtgataac cgttttgtgc gcgtgagctg
     3781 ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtacagaaaa ctcacgggcc
     3841 gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg
     3901 gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc
     3961 taccggtgct gcgcaggtga tcctgccgcg cgtagtcggt tcaatggaag tgtatgaaca
     4021 gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctccga
     4081 tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta
     4141 cgagcagcta aaagcgaaag tcgccgccgg tgaagttgag gtcatcagca tcgatccggt
     4201 tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc
     4261 gcaaactgac gtgccgctgc aactggcgct ggcacatacg ctgtacagtg aaaacctgta
     4321 cgacaaaaac ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtatctgct
     4381 gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga
     4441 tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc
     4501 tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct
     4561 ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggt tttggctggc actacaacgg
     4621 cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct ctacgtcgat
     4681 tccgcctgtt cacgacaaca gtgactacaa aggctacagc agcactattc cgattgcccg
     4741 ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta aatcggtaaa
     4801 actgccgccg ctgaaaatgt gtatttttgc cggaactaac ccattccatc gccatcagca
     4861 gatcaaccgc attattgaag gctggcgcaa gctggaaacg gttatcgcca tagataacca
     4921 gtggacctca acctgccgct ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg
     4981 taacgatctc gaccagtacg gcaaccactc caaccgtggc attatcgcca tgaaacaggt
     5041 ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc tgtgccgtcg
     5101 ctttaatcgt gaagaagcct ttaccgaagg gctggacgaa atgggctggc tgaaacgcat
     5161 ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt catctgccag cgtttgatga
     5221 cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg ttcgccacca
     5281 ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc tgattgagat
     5341 ctactcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc cgatgtggtt
     5401 tgagaaaatc gaacgctccc acggtgggcc cggctcgcag aagtatccgt tgcatctgca
     5461 atctgtgcat ccggatttcc gacttcactc gcagctatgt gagtcggaaa cgctgcgtca
     5521 gcaatatacg gtagcgggta aagagccagt gttcattaac ccgcaggatg ccagcgcgcg
     5581 cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg
     5641 agcagtagtt tctgaccgct atgcacccgg cgtggcgcga attcacgaag gggcatggta
     5701 cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgct
     5761 gaccatcgac atcgggactt cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt
     5821 ggaaattgag aagtacaatg gaacagtgga gcaggtgacg gcgtttaacg gccccgtgga
     5881 gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca
     5941 gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt tgttctcccg tgaactggac
     6001 gatgaacaac tgacgcaaat cgccagtgcg cagatggctg aatggttttc gttgctgaaa
     6061 agcgaaccgc cgctcactgc ggcggtgaat gatctggaaa acagtattgc cac
//
LOCUS       DAECOD010000162         6052 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.162, whole genome shotgun sequence.
ACCESSION   DAECOD010000162 DAECOD010000000
VERSION     DAECOD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.162"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            430..1338
                     /locus_tag="KKO15_003722"
     CDS             430..1338
                     /locus_tag="KKO15_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBD6362348.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KKO15_003723"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KKO15_003723"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD6362349.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKQAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="KKO15_003724"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="KKO15_003724"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBD6362350.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KKO15_003725"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KKO15_003725"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBD6362351.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWSFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="KKO15_003726"
     CDS             complement(5673..>6052)
                     /locus_tag="KKO15_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362352.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1577 c         1562 g         1422 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatat tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttgtttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ctccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAECOD010000163         5891 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.163, whole genome shotgun sequence.
ACCESSION   DAECOD010000163 DAECOD010000000
VERSION     DAECOD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.163"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            51..245
                     /locus_tag="KKO15_003727"
     CDS             51..245
                     /locus_tag="KKO15_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBD6362353.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            512..1438
                     /gene="tauA"
                     /locus_tag="KKO15_003728"
     CDS             512..1438
                     /gene="tauA"
                     /locus_tag="KKO15_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6362354.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1451..2218
                     /gene="tauB"
                     /locus_tag="KKO15_003729"
     CDS             1451..2218
                     /gene="tauB"
                     /locus_tag="KKO15_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBD6362355.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2215..3042
                     /gene="tauC"
                     /locus_tag="KKO15_003730"
     CDS             2215..3042
                     /gene="tauC"
                     /locus_tag="KKO15_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBD6362356.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3039..3890
                     /gene="tauD"
                     /locus_tag="KKO15_003731"
     CDS             3039..3890
                     /gene="tauD"
                     /locus_tag="KKO15_003731"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBD6362357.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3996..4970)
                     /gene="hemB"
                     /locus_tag="KKO15_003732"
     CDS             complement(3996..4970)
                     /gene="hemB"
                     /locus_tag="KKO15_003732"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBD6362358.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5494..5862
                     /locus_tag="KKO15_003733"
                     /pseudo
     CDS             5494..5862
                     /locus_tag="KKO15_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1456 c         1598 g         1438 t
ORIGIN      
        1 cagaaatgtt gtcgtttctt accagtgagg aacgagaagc ccttacaaac atgctcgcaa
       61 ggaaaggtgt tgagactgtg ctacgcttgt tagataagga aactcaagag ctactgcaac
      121 tagagggccg taaacgcctg gcagcgttga tgctcgttga tatgcccgac gagcaagtca
      181 gagagatttt ggaagagatt gaagctagta ttctctcaga gagtgtaaac gcgaaggcgg
      241 gttaacttca ttgacctgcc tttagggtgc ctttaaaatt cgtttttaaa ggctacgatg
      301 acatttttga agagtcttaa aagaattaaa atacacggcg cgcctatctt gaatgtttta
      361 tcgatatcga agcattgagc atttttaact cgttttatct tgaatgcaat cggcgcataa
      421 cttattgaat cccatcaaat cccgtctttt tttacccatt cccggttatc ttgaataaat
      481 tacctagtca tataacacac ttcttgccgc actggcattc atcgcttttc aggcacaggc
      541 ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga
      601 caacaccttt gctaaagaga gcggggcgag cgtggactgg cgtaagtttg acagcggagc
      661 cagcatcgtg cgggcgctgg cttctggcga cgtgcaaatc ggcaacctcg gttccagccc
      721 attagcggtt gcagccagcc aacaggtgcc gattgaagtc ttcttgctgg cgtcaaaact
      781 gggtaactcc gaagcgctgg tggtaaagaa aactatcagc aaacccgaag atttgattgg
      841 taaacgcatt gccgtaccgt ttatctccac cacccactac agcctgctgg cggcgctgaa
      901 acactggggg attaaaccag ggctagtgga gattgtgaac ctgcagccgc ccgcgattat
      961 cgccgcatgg cagcggggtg atattgatgg tgcttatgtc tgggcaccgg cggttaacgc
     1021 cctggaaaaa gacggcaagg tgctgaccga ttctgaacag gtcgggcagt ggggtgcgcc
     1081 aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa
     1141 agcgttcgcc aaaagcgcca tcgatgcaca gcaaccgtac attgctaacc ctgacgcgtg
     1201 gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga
     1261 cgttccgggg ctggtgaagg gaaataccta tctgacgccg cagcaacaaa cggcagaact
     1321 gaccggaccg gtgaacaaag caatcatcga caccgcgcag tttttgaaag agcagggcaa
     1381 ggtcccggct gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa
     1441 ggaggcgcag atgctgcaaa tctctcatct ctatgccgat tatggcggca aaccggcact
     1501 ggaagatatc aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg
     1561 ctgcggtaaa accaccctgc tgaatctgat tgccggtttt gtgccttatc agcatggcag
     1621 cattcaactg gcgggtaagc gtattgaggg accgggagca gagcgtggcg tagtttttca
     1681 gaatgaaggg ctgctaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt
     1741 ggcaggtata gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg
     1801 gctggaaggc gcagaaaaac gctatatctg gcagctttcc gatggtcaac gtcagcgggt
     1861 ggggattgcc cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg
     1921 tgcgctggac gccttcaccc gcgaccagat gcaaactctg ctgctgaaac tctggcagga
     1981 gacgggcaag caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac
     2041 tgaactggtt ctgctttcat ccggccctgg ccgtgtgctg gagcggctgc ggctcaactt
     2101 tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat
     2161 cgccatgcgc gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt
     2221 gtgctcatta atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt
     2281 caggtgacct taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg
     2341 gcgctgcaac tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg
     2401 ctcaccattg ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc
     2461 agcctgacgc gcattgtgct ggcgctactc gcggcggtgg tgatcggtat tccggtcggg
     2521 atcgccatgg ggctaagccc gacggtgcgc ggcattctgg atccggtaat cgaactttat
     2581 cgtccggtgc caccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa
     2641 acctcgaaga tcttactgat ctatttagcg atttttgcgc cggtggcgat gtcggcgctg
     2701 gcaggggtga aaagcgcgca gcaggttcgc attcgtgccg cccagtcgct gggtgccagc
     2761 cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga
     2821 ttacgcattg gtctgggtgt gggctggtct acgctggtag cggcggagct gattgccgcg
     2881 acacgcggtt taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggta
     2941 ctggcgggga tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg
     3001 ttacagcgcc gtctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta
     3061 ccccgctggg gccgtatatc ggcgcacaaa tttcgggtgc cgacctgacg cgcccgttaa
     3121 gcgataatca gtttgaacag ctttaccatg cggtgctgcg ccatcaggtg gtatttctgc
     3181 gcgatcaagc tattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc
     3241 atattcaccc tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc
     3301 ataacgataa tccgccggat aacgacaact ggcataccga tgtgacattt attgaaacgc
     3361 cacccgcagg agcgattctg gcagctaaag agttaccttc gaccggcggt gatacgctct
     3421 ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagct
     3481 ggctgcgtgc ggagcatgat ttccgtaaat cattcccgga atacaaatac cgcaaaaccg
     3541 aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa tcctccgttg ctacatccgg
     3601 tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta
     3661 cgcgaattgt cgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc
     3721 atatcactaa accggagttt caggtgcgtt ggcgctggca accgaatgat attgcgattt
     3781 gggataaccg cgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta
     3841 tgcatcgggc gacgatcctt ggggataaac cgttttatcg ggcggggtga tacgagtgcg
     3901 gacggttccc tcgccccctt ggggagaggg ttagggtgag ggggcgttca ctgtactttc
     3961 aacaggttaa ctcccccttt ctgagaggaa aaaaattaac gcagaatctt cttctcagcc
     4021 aaatccagcg caaagtagct gaaaatcaga tccgcacccg cacgcttaat tgaacctaag
     4081 ctttcgagca cgactttctc ttcatctata gcacccgcca gcgcggcgaa cttaatcatc
     4141 gcgtactcac cgctcacctg atacgcgcca atcggcaatt cagtacgttc acgcagctca
     4201 cgcacgatgt cgaggtacgc tccagcaggt ttaaccatca ggcagtctgc gccctgggct
     4261 tcatccagca gtgactcacg aatcgcctcg cgacggttca ttgggttcat ctgatagctt
     4321 ttgcggtcgc cttttaatgc gcttcctgca gcttcacgga acgggccata aaaggaagag
     4381 gcgaacttgg tcgaatacga cataatcgcc gtatctttaa agcctgcagc gtccagcgcc
     4441 tgacgaatcg cctgtacctg gccgtccatc gcggcggaag gggcgatgaa gtctgcgcct
     4501 gcagcagctg caaccacggc ttgcttgcct aaattttcca gagtcgcgtc gttgtcgacg
     4561 ccatgctcgc acagcacacc gcagtgaccg tgagaagtgt attcacagaa acaagtgtct
     4621 gacatgacga tcatttctgg cacagtctgc ttgcagatgc gcgacatacg cgccaccagt
     4681 ccatcttccc gccaggcgtc gctgccggtt tcatcggtat ggtgagagat gccaaaagtc
     4741 attacggaac ggataccggc gttggcgatg cgttcaattt cgcgtgccag atgtttctct
     4801 ggaatgcgca tcacgcctgg catggcttca acggctttgt agtcgtcaat ttcttcttca
     4861 acaaagatcg gcaacaccag gtcgttaagg ctaagtgttg tctcttcaaa catagcgcgc
     4921 agcgcaggag atttgcgcag gcgacgaggg cgttggatta agtctgtcat ggtctgcctg
     4981 atgtttgtgg aattaaggtg cacagtatac ctgaagcggg gcaggagtgt tttacgaaag
     5041 ttgtcgcgat gttgacaaga agagaatgaa agagagacca ggacatcttt gtaatagaaa
     5101 tagcaataat ttcatattca ctgaaaatat ttttaatctt tatttatagc tcttggttat
     5161 tattttttgg cgtttggttg tgctgctata aattgaataa ttcaaacttt gttgtatatt
     5221 gataagatat ttatctggtt tgtgtttttg atatttataa cgcgtatgca tcaaaaataa
     5281 cattatctgc aacatactga aatatcgtcc ttttatcaat ttgatgcgtg tttttctcca
     5341 taaattatat atgtccacat tcggacttag gggaaagaat aattgaacca ttcgtctgta
     5401 acgcagcata atcgttagcg cgaaacataa tatgttttct atgcattgat aattgatgga
     5461 tcaacttatt acgtccctga ggagggatga caaatgcact cctggaaaaa gaaacttgta
     5521 gtatcacaat tagcattggc ttgcactctg gctatcacct ctcaggctaa tgcagcgacc
     5581 aacgatattt ctggtcaaac ttacaatact ttccatcact acaacgacgc cacctatgct
     5641 gatgacgttt actatgatgg ttatgtaggc tggaacaact atgccgctga tagctattac
     5701 aacggcgata tctacccggt cattaataac gctaccgtta acggcgtgat ttctacctac
     5761 tatctggacg acggtatttc taccaatacc aacgccaata gtctgacaat caaaaacagc
     5821 actattcacg gtatgattta ttccgagtgc atgacaactg attagactgg ccccctgaat
     5881 ctccagacaa c
//
LOCUS       DAECOD010000164         5785 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.164, whole genome shotgun sequence.
ACCESSION   DAECOD010000164 DAECOD010000000
VERSION     DAECOD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.164"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..942)
                     /gene="hprS"
                     /locus_tag="KKO15_003734"
     CDS             complement(<1..942)
                     /gene="hprS"
                     /locus_tag="KKO15_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBD6362359.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNK"
     gene            complement(942..1613)
                     /gene="hprR"
                     /locus_tag="KKO15_003735"
     CDS             complement(942..1613)
                     /gene="hprR"
                     /locus_tag="KKO15_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBD6362360.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1746..2159
                     /gene="hiuH"
                     /locus_tag="KKO15_003736"
     CDS             1746..2159
                     /gene="hiuH"
                     /locus_tag="KKO15_003736"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD6362361.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2268..3272
                     /gene="msrP"
                     /locus_tag="KKO15_003737"
                     /pseudo
     CDS             2268..3272
                     /gene="msrP"
                     /locus_tag="KKO15_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3273..3908
                     /gene="msrQ"
                     /locus_tag="KKO15_003738"
     CDS             3273..3908
                     /gene="msrQ"
                     /locus_tag="KKO15_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBD6362362.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4166..4816
                     /gene="zinT"
                     /locus_tag="KKO15_003739"
     CDS             4166..4816
                     /gene="zinT"
                     /locus_tag="KKO15_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBD6362363.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1306 c         1195 g         1641 t
ORIGIN      
        1 cttattgaga gaaagcgaat ccagtttcac caaaacattg tttttgtctg ccctggcaag
       61 aaacagtata ttctctgtta accgcgaaat attttccagc tcttcaatgt ttccggcaat
      121 tgttttttga tattcagcga tacttctgat ttgactgagc gtaacctgat tctgacccag
      181 taatgcatta attggcgttc taagttcatg agcgagatcg tcagcaaatt gactaaggcg
      241 ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa gaggttttag
      301 ttctctcggt aatgcgttaa cctcaaccgg cactcggcta tcgttataat tcagcgcttc
      361 cgttacgcca ctcaactttt tgatctctcg caaccccgtc ctgattaaca acggacttaa
      421 tactgagcac agtataatgg cgacaatgca aattataatg ctattaattt tatactgttc
      481 aagcatatta tgtctggctg aagccaattt agccacagta accgttaatg atggcgaaac
      541 ttcatcaatg ttaattcgta aagcatctat ctctgtatca ttaataatgc ttctgtaaat
      601 gcctgctgcg ctgattgtct cactagcagg tatgttattt aacatgtcat cactgacatt
      661 tgtccggtta acaattttat tgatgccatc actatgaatg atcaaaatat cctgactaac
      721 atccatcatc cggttaaagt acacaggtaa cgtatctgga tttaccccat caattaacaa
      781 ctgcttgatc tgcgctgtcc ggttaatgag tgttgtatca tcgcgccatt tcaactcact
      841 tgccaggcca ttatagagag tccatacaat tccagcgcca gcaacagact gcagaaatat
      901 aaaaagcaag gttaaacgga cggttataga taatcttttc attatttttt taccgctacg
      961 aatgaatagc ccatcccccg gattgtggcg attagctttt caggaaaagg atcatcaact
     1021 tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc aaagttgatt
     1081 ccccaaattt cactcgcaat aaccgttctg ggtataatct cgccagctct ggaggccagt
     1141 agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtc cctgctgaca
     1201 ctttgactga cagagtccat ttttaagcca ctgatttcta atgttgaatt cagagcgtga
     1261 tgttgcctta attgtgcccg aacccttgct agcaactcag aaaatgaaaa aggttttacc
     1321 agataatcat ttgccccact gtccaaccct ctgactctgt catcgacaga atcccttgca
     1381 gtaaggcaaa taacaggggt ttgctttgcc gttcttaacg tttgtaagat ctgccagcca
     1441 tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt cagcgcaaga
     1501 taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc ggaaagcccc
     1561 tgcgttaccc attcctgggt cctttgatta tcttcaataa gtagaatctt catgaaaata
     1621 atatgccata aatcatatgt tatagaaaca acctggttca ttacaaaatt gtaatgctgc
     1681 tgtaaggtta ccctggacgt tttttcgcta tcctcaaaac tcattcacat gacaaggata
     1741 taaacatgtt aaagcgttat ttagtactct cggtagcaac ggcagcattt tcattacctt
     1801 ctttggttta tgccgcacaa caaaacattc ttagcgtgca cattttgaac cagcaaacag
     1861 gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggataac ggctggttac
     1921 aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg cccgagcaaa
     1981 ctgcgactac tggcgattac cgtgtcgtat ttaaaaccgg ggactatttc aagaaacaaa
     2041 atcttgaaag tttcttccct gagattcccg ttgaatttca tattaataaa gtgaacgagc
     2101 attatcatgt gcctttgtta ctcagccaat atgggtattc aacctatcgt ggcagttaat
     2161 gcgtaaatat gtaaaagctg atctctgcta ctgttaggga ggtgactccc tgacagatta
     2221 acagtaaaca gctcttgttg gttaacgaca aaaaagtgtg atggcttatg aaaaagaagc
     2281 aatttttaaa agaatcagat gttacggccg agtcggtatt ctttatgaag cgtcggcagg
     2341 tgttaaaagc actgggcatc agcgcagctg cattttcttt gcctcacgct gcgcatgccg
     2401 atctgcttag ctggtttaaa gggaacgatc gtccgcccgc ccccgccgga aaaccgctgg
     2461 agttcagcaa gcctgccgcc tagcaaaata acctgccact gacgccagca gataaagtct
     2521 ccggttataa caacttctat gaattcgggc tggataaaga cgatcccgcg gctaatgctg
     2581 gtagcctgaa aaccgatcca tggacactga aaatcaacgg cgaagtggca aaaccattga
     2641 ccctcgatca tgatgattta acccgtcgct tcccgctgga agagcgtatt tatcgtatgc
     2701 gctacgtgga agcgtggtcg atggtggtgc cgtggattgg ttttccgcta cacaaattgc
     2761 tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaacg atttatgcgc
     2821 cggaacagat gcctggccag caggaccgct ttatcggcgg cgggctgaaa tatccttatg
     2881 tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc gtaggtgttt
     2941 atggcaaggc gttaccaccg caaaatggcg cgccggtgcg actgattgtg ccgtggaaat
     3001 atggctttaa agggattaaa tcgatcgtca gtattaagct gacccgcgag cgtccgccaa
     3061 ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt aatccgcatg
     3121 ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc ggcattctcg
     3181 atgttcagcg ccagccaaca ctactgttta atggttacgc cgaccaggtg gcatcgctgt
     3241 atcgtggcct ggatttgcgg gagaatttct aaatgcgtct gacagcaaaa caagtgatat
     3301 ggctgaaagt ttgcctgcat cttgccggat tgttgccgtt tctctggctg gtctgggcga
     3361 tcaatcacgg tggactgggt gccgatccgg tgaaagatat tcagcatttt accggtcgca
     3421 ctgcactgaa atttttgctt gcggccttgt taatcacacc tctggcacgc tacgcaaaac
     3481 agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc tgggcgacac
     3541 tgcatttaac cagttacgca ttgctggagt tgggcgtgaa taatctggcg ttattaggta
     3601 aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt attttgcttg
     3661 ctttagcgtt cacttcaacc cagtcgatgc agcgaaaact cggcaaacac tggcaacagt
     3721 tgcataactt cgtctatctt gtcgcgatcc tggccccaat acattatctg tggtcagtga
     3781 agattatttc accgcagcca ctcatctacg ctgggctggc tgtactgctt ttagccttac
     3841 ggtataagaa gttgctttcc cttttcaacc ggttacgcaa gcaggcccac aacaaattat
     3901 ctctataact tcatattgcc gacaaacttc gcgttatccc ggccggatgc ggcgcgagcg
     3961 ccctatccgg cccacacaga tcgcatcaag ttatgtttcg tttgtcatca acctttcatc
     4021 accagaataa tttattttat ttcaataaga tagttcaata cacctgagaa taccatgctc
     4081 tcatttccta atgagttgtt gcattttttc atatgttaca atataacatt acacataatg
     4141 tacattaact ctggaggaaa ttgttttggc gattcgtctt cataaactgg ctgttgcttt
     4201 aggtgtcttt attgttagcg ctcctgcctt ttcgcatggt catcattctc atggcaaacc
     4261 cttaacagag gtcgaacaaa aagctgctaa tggtgttttt gatgatacca atgtacaaaa
     4321 ccgaacgctc agtgactggg atggagtctg gcaatccgtt taccctttac tgcaaagtgg
     4381 caaacttgat cccgtctttc agaagaaagc ggatgcagat aaaaccaaaa catttgctga
     4441 aattaaagat tattatcgca aaggttatcc gacagatatc gaaatgattg gcattgagga
     4501 cggcattgtc gaattccata gaaataatga aacaacatcc tgtaaatatg attacgatgg
     4561 atacaaaata ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa
     4621 agatcctgaa agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg
     4681 aaaatcatct cattttcaca tttttatggg taatgattcc cagcaatctt tattgaatga
     4741 aatggaaaac tggccaacgt attatcccta tcagttgagt agcgaagaag tggtcgagga
     4801 gatgatgtct cattgatttt tttgcgctga cttcagttat ttaacagaac agtgcctggt
     4861 agtaacaaca ataatttacc aagacaaata agtaagacaa actcagacac gctcatactt
     4921 gagtcgtgtc ttttttatta tgtctgtaga aaaaaactga gaaaaaggct aacaataaat
     4981 acacaactca gccatacaga ctggcagcgc aaatgtttcc aggagcaagt catctgtcat
     5041 ggtaaagcta accagaaata taggttaatt ttggtgttta aacctttttt acttgttagt
     5101 atgatacaaa ggctttcaaa aaagctggga aattcaacaa ctcttggaaa ttatggactt
     5161 tgtcccgcgg ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc
     5221 tctacgcaac ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa
     5281 aagaataatg gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct
     5341 tcctgtcagc tccatagcga atcaagcgct gaatgtcaca gtatcgaaca gaaaacagtg
     5401 acgatctaac ccttcaagaa tattctacga ttgttctgtt taggaaaagc aaggcgggaa
     5461 gtcgggagat aagtcattga taaaatggcg gagagagggg gatttgaccc cccggtagag
     5521 ttgcccctac tccggttttc gagaccgatg atacagagtc gaaaaatcaa agagttaaaa
     5581 atttttcttg gaaaaaatgt cttaaaatcg tacacgaaaa atcaatgaat tacataacat
     5641 taaagaagaa tattccaagc cttcttgagt ccaccacctg ttttcaggtg tcattcagac
     5701 gtgatttgcc ccagattggg aaagcacgct tttttctcag cttattccgt tttgcctgtg
     5761 tgttgaataa ttccactgca gttat
//
LOCUS       DAECOD010000165         5702 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.165, whole genome shotgun sequence.
ACCESSION   DAECOD010000165 DAECOD010000000
VERSION     DAECOD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.165"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..170)
                     /locus_tag="KKO15_003740"
     CDS             complement(<1..170)
                     /locus_tag="KKO15_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362364.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="KKO15_003741"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="KKO15_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBD6362365.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="KKO15_003742"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="KKO15_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="KKO15_003743"
     CDS             complement(3616..4383)
                     /locus_tag="KKO15_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6362366.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="KKO15_003744"
     CDS             4579..5487
                     /locus_tag="KKO15_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD6362367.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            5484..>5702
                     /locus_tag="KKO15_003745"
     CDS             5484..>5702
                     /locus_tag="KKO15_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBD6362368.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1411 a         1470 c         1482 g         1339 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gatctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggattgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttattgca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcccaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggca
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAECOD010000166         5655 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.166, whole genome shotgun sequence.
ACCESSION   DAECOD010000166 DAECOD010000000
VERSION     DAECOD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.166"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            190..447
                     /locus_tag="KKO15_003746"
                     /pseudo
     CDS             190..447
                     /locus_tag="KKO15_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="KKO15_003747"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="KKO15_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBD6362369.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="KKO15_003748"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="KKO15_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBD6362370.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="KKO15_003749"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="KKO15_003749"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBD6362371.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="KKO15_003750"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="KKO15_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="KKO15_003751"
     CDS             complement(3605..4420)
                     /locus_tag="KKO15_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6362372.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..5164
                     /locus_tag="KKO15_003752"
     CDS             4517..5164
                     /locus_tag="KKO15_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6362373.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVHV"
     gene            complement(5328..>5655)
                     /locus_tag="KKO15_003753"
     CDS             complement(5328..>5655)
                     /locus_tag="KKO15_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6362374.1"
                     /translation="LTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLLQVYRAL
                     GMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISL
                     LRYMPA"
BASE COUNT         1478 a         1342 c         1330 g         1505 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc ttgttcacgt
     5161 ataggaaatt gaaaaaccag acgataccgc tgtccacgcc ttatgccacg tgagcttttc
     5221 tgcttttccc tccactccgg tgttttctct ctctgcatgg tcgattctta taccgcaaca
     5281 ggctgttttt tatacttgca ccgattcccg ggcacactta gcctgtgtca ggccggcata
     5341 tacctcagca gactgatatc ccgcgcgttt ttcttcagcc cctccacatt cagtcccgcg
     5401 atggcccggc ggtataccat ccggccgcca cacagcacgc attcgaacgg gtcacgactc
     5461 aggaactgtt tcaccatttg tgcatagcac actttcgcca ctggttccgg tttatccatc
     5521 cccagagcac ggtacacctg cagcagcttc tctccacaca cccggttggc aaggaacccg
     5581 aagtacctca ccatcttaaa aaacttctcc gggatgtgct gtttcagcct cgcgaccagc
     5641 tcacgctgtg tcagc
//
LOCUS       DAECOD010000167         5599 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.167, whole genome shotgun sequence.
ACCESSION   DAECOD010000167 DAECOD010000000
VERSION     DAECOD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.167"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="KKO15_003754"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="KKO15_003754"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBD6362375.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="KKO15_003755"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="KKO15_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBD6362376.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="KKO15_003756"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="KKO15_003756"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBD6362377.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="KKO15_003757"
     CDS             4129..4884
                     /locus_tag="KKO15_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBD6362378.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="KKO15_003758"
     tRNA            4963..5036
                     /locus_tag="KKO15_003758"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
BASE COUNT         1381 a         1411 c         1456 g         1351 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 ctttcttaaa gtactccaac ctactgaatc ctaagggata tggctttaag tctccttgca
     5101 gagttactta ctgtaaccca ctgtgtaacc ctcagggcat acagacatat gattcagcta
     5161 tccagtagtc acaaacttcc agcagtctac tatctctacc agcgtaacgg cgtctactac
     5221 ttccgtctta gggtcagaca atcaaacaat gatagaatga cctcaatatc attacggacc
     5281 aaggatagac gcaccgctat ggctcactca aggcacataa aggcagcact caaggcaata
     5341 cacgcagaca gacccaacgc tacctatgag gagatgaggg agcaccttaa ggacatcgct
     5401 gagtgtgaac tgtctatggg ccgttcagat ctctttgagc ctgacatgag ggatatctac
     5461 cgggaccaat acggagaact tggtgagagc cttaccgatg ctctggctag tgagccgcta
     5521 agcattgacc agcatcgtta catcaatgag gccctgaagg tgctaaaagc ctgtatgagg
     5581 cgtatcgaag cgggagata
//
LOCUS       DAECOD010000168         5591 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.168, whole genome shotgun sequence.
ACCESSION   DAECOD010000168 DAECOD010000000
VERSION     DAECOD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.168"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..279)
                     /locus_tag="KKO15_003759"
     CDS             complement(1..279)
                     /locus_tag="KKO15_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362379.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KKO15_003760"
     CDS             220..405
                     /locus_tag="KKO15_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362380.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KKO15_003761"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KKO15_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6362381.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KKO15_003762"
     CDS             complement(1254..1670)
                     /locus_tag="KKO15_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362382.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KKO15_003763"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KKO15_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="KKO15_003764"
     CDS             1837..2862
                     /locus_tag="KKO15_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6362383.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="KKO15_003765"
     tRNA            complement(2915..3004)
                     /locus_tag="KKO15_003765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="KKO15_003766"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="KKO15_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBD6362384.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="KKO15_003767"
     tRNA            3996..4071
                     /locus_tag="KKO15_003767"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="KKO15_003768"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="KKO15_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1327 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgcc
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct a
//
LOCUS       DAECOD010000169         5557 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.169, whole genome shotgun sequence.
ACCESSION   DAECOD010000169 DAECOD010000000
VERSION     DAECOD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.169"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..641
                     /locus_tag="KKO15_003769"
     CDS             <1..641
                     /locus_tag="KKO15_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBD6362385.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KKO15_003770"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KKO15_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBD6362386.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="KKO15_003771"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="KKO15_003771"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBD6362387.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAECOD010000170         5399 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.170, whole genome shotgun sequence.
ACCESSION   DAECOD010000170 DAECOD010000000
VERSION     DAECOD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.170"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..497
                     /locus_tag="KKO15_003772"
     CDS             <1..497
                     /locus_tag="KKO15_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBD6362388.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="KKO15_003773"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="KKO15_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="KKO15_003774"
     CDS             2500..3114
                     /locus_tag="KKO15_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBD6362389.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="KKO15_003775"
     CDS             3114..3443
                     /locus_tag="KKO15_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBD6362390.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="KKO15_003776"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="KKO15_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBD6362391.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="KKO15_003777"
     CDS             4530..4841
                     /locus_tag="KKO15_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD6362392.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..4983
                     /locus_tag="KKO15_003778"
     CDS             4825..4983
                     /locus_tag="KKO15_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD6362393.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4976..5375
                     /locus_tag="KKO15_003779"
                     /pseudo
     CDS             4976..5375
                     /locus_tag="KKO15_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1343 c         1425 g         1454 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcata cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tcttggcgtc cttcagggtt
     5101 cacaaccttc agaaattggt gttgtcagat tagtcatgtt ggggggagcg cggaagtatc
     5161 aaaagcgggg ctttggtcag gacctactat gtgatttttt tgaacatgta aaaaaaattc
     5221 acccggcatt accaattaaa ggggtttatc ttgatgctga ccctgccgcc attaactttt
     5281 atgctcgtct cggctttgtt cagctttcag cgacaccaaa tgcttttggt gctgtaccta
     5341 tgtttttggc gattcagcat attctcgcgg cttagattat tatcgccacc cgccagggc
//
LOCUS       DAECOD010000171         5339 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.171, whole genome shotgun sequence.
ACCESSION   DAECOD010000171 DAECOD010000000
VERSION     DAECOD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.171"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="KKO15_003780"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="KKO15_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBD6362394.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="KKO15_003781"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="KKO15_003781"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBD6362395.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="KKO15_003782"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="KKO15_003782"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBD6362396.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     AENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="KKO15_003783"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="KKO15_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBD6362397.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1309 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatgcg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAECOD010000172         5315 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.172, whole genome shotgun sequence.
ACCESSION   DAECOD010000172 DAECOD010000000
VERSION     DAECOD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.172"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(102..1862)
                     /locus_tag="KKO15_003784"
     CDS             complement(102..1862)
                     /locus_tag="KKO15_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBD6362398.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSKWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1992..2618
                     /locus_tag="KKO15_003785"
                     /pseudo
     CDS             1992..2618
                     /locus_tag="KKO15_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2817..3905
                     /gene="serC"
                     /locus_tag="KKO15_003786"
     CDS             2817..3905
                     /gene="serC"
                     /locus_tag="KKO15_003786"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBD6362399.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVSSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3976..5259
                     /gene="aroA"
                     /locus_tag="KKO15_003787"
     CDS             3976..5259
                     /gene="aroA"
                     /locus_tag="KKO15_003787"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBD6362400.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1328 a         1263 c         1364 g         1360 t
ORIGIN      
        1 attttattaa tgcaaatata tgtaaagcgg ggcattaaaa aaacgccccg taatataact
       61 cagactaatc aaatagccta cattgcgtag gctatttgat tttattttgc ccagaatgct
      121 gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg actgatgtgc agcaaacgcg
      181 tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg cttcgccgct cattgccgca
      241 ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa acgcattcag atattgcagc
      301 ggctggcgat cttcttcctg tgccagtaat aacaacgttt gcaggcagcg ataatagttg
      361 gcgcgttccg gactaaatac tgatgagtta aactccatcg tccattcggt ccagaccaga
      421 gcctgttcca gatcgccacc agccagcgcc agcatggctt ttaattcacc gatacgcagg
      481 gtgtaccaac cgttatccga cccggtcgcc agacccaaca gctcacgcac gcgggtaaag
      541 tcatcaaaat cttcttcatc cagttgctcg atgaggttca ggtaatcttc tttttcccac
      601 ttgctgcctg gtagcgaaag aatcgtttca cgtaaatggc tgcccatact gttattcgcg
      661 agccacagat cttcagccgg ataaatatcg gacatgccag gcacgataat acggcaagca
      721 taaacgccca gatgctcgta atcggcaata taaacttctt tatcttcttt gttgaagata
      781 gccatcagcg tagcaaactc ttcttccgtg gtgccggaga aattccagtc cacaaacgga
      841 taatcggcat cctgcttgaa caggtcccag gagattaaac cgctggaatc gataaagtgc
      901 gtttcgaggt tggtatgttc agcgacttct tcatcatcga aggttggcgg agtaaacaca
      961 tccaaatctt tcaggccacg accttgcagc agctcggtca cggtacgttc cagtgctacg
     1021 ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc cgtttgcagg attaaacagt
     1081 accacgcaaa tcaccggata ctggccgcca agggaaccat catatgcgaa gatcgggaaa
     1141 ccttctgttt ccagtgtttc gatcgcttca accaccgctg ggtaacgcgc cagcacgtct
     1201 gccgggattt ccggcaagct gatgctttca gcaataatgc ggtttttcac gtagcgttcg
     1261 aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg tattgccagc ggacataccg
     1321 ttagaaacgt acaggttacc aatgatattc atcggaatat aaacggtctg attgtcggac
     1381 tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt taccggattg taggtcaatc
     1441 agcatactgc cggtcaattc attctccgga tcgtaaaatg cgcgcagacg gtcatcgagc
     1501 agcccttctg gcacatcgtc attttcgctc agtgggaacc atttttcgtt gggataatgc
     1561 acaaatggac cgttggcgat ggtttcgccc agccagaaat cagcaaaaaa gtagttggtt
     1621 gagagacgct cgaaatattc accgagtgca gaagccagcg ccgctttctt ggttgcgcct
     1681 ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa tatgtacaga ccagacgtta
     1741 ggcacgggat tcagccagga ggcctcttca atctgaaagc cgaggtctga aagtttttgc
     1801 tggaagcgag cgatggaatc ttccagagcg gcatctttgc cggggataaa tgtttgcgtc
     1861 atgaaaatca ctttagtcgt acggaaagcg cgcaataata cgggttttat ctcaaaggcg
     1921 ctatcaccgc cgccatgccg atgaactgtt gactatgctt ttagcggata aaccacgtta
     1981 agagcataaa aatgaaggcg ttcgatctcc accgtatggc atttgataaa gtgccttttg
     2041 atttccttgg cgaagttgca ctacgtagtc tttatacctt tgtactggtt ttttgttcct
     2101 caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg tttgaagtgt taatcattct
     2161 cacgctgggt tcagcggcgg gagatgtggc gttttatgat gatgtgccga tggtgccggt
     2221 acttatcgtc tttatcactc tggcgttgtt ataccgcctg gtaatatgat ggcgagctgg
     2281 cctggtcgaa actcaataac tccaacatga cggaatttga gttctttatg gagctacgat
     2341 tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat tctcgaaacc aacgggcaaa
     2401 tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg tttacttatt ttacccagtg
     2461 attgtacgca gcgttacaaa gtggtgccgg agtcggcgga ctatgcctgc attcgttgta
     2521 gtgaaatcat tcatatgaac gcgggggaaa aacaattatg tccgcgctgt gcaaatccag
     2581 aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca gtaaaaacat cggctttttg
     2641 ctaataatcc gagagattct tttgtgtgat gcaagccaca tttttgccct caacggtttt
     2701 actcattgcg atgtgtgtca ctgaatgata aaaccgatag ccacaggaat aatgtattac
     2761 ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc aacgtggtga ggggaaatgg
     2821 ctcaaatctt caattttagt tctggtccgg caatgctacc ggcagaggtg cttaaacagg
     2881 ctcaacagga actgcgcgac tggaacggtc ttggtacgtc ggtgatggaa gtgagtcacc
     2941 gtggcaaaga gttcattcag gttgcagagg aagccgagaa ggattttcgc gatcttctta
     3001 atgtctcctc caactacaag gtattattct gccatggcgg tggtcgcggt cagtttgctg
     3061 cggtaccgct gaatattctc ggtgataaaa ccaccgcaga ttatgttgat gccggttact
     3121 gggcggcaag tgccattaaa gaagcgaaaa aatactgcac gcctaatgtc tttgacgcca
     3181 aagtgactgt tgatggtctg cgcgcggtta agccaatgcg tgaatggcaa ctctctgata
     3241 atgcggctta tatgcattac tgcccgaatg aaaccatcga cggtatcgcc atcgacgaga
     3301 cgccagactt cggcaaagat gtggtggtcg ccgccgactt ctcttcaacc attctttccc
     3361 gtccgattga cgtcagccgt tatggtgtaa tttacgctgg cgcgcagaaa aatatcggcc
     3421 cggctggcct gacaatcgtc atcgttcgtg aagatttgct gggcaaagcg aatatcgcgt
     3481 gtccgtcgat tctggattat tccatcctca acgataacgg ctccatgttt aacacgccgc
     3541 cgacatttgc ctggtatcta tctggtctgg tctttaaatg gctgaaagcg aacggcggtg
     3601 tagctgaaat ggataaaatc aatcagcaaa aagcagaact gctatatggg gtgattgata
     3661 acagcgattt ctaccgcaat gacgtggcga aagctaaccg ttcgcggatg aacgtgccgt
     3721 tccagttggc ggacagtgcg cttgacaaat tgttccttga agagtctttt gctgcaggcc
     3781 ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg cgcgtctatt tataatgcca
     3841 tgccgctgga aggcgttaaa gcgctgacag acttcatggt tgagttcgaa cgccgtcacg
     3901 gttaatgccg aaattttgct taatccccac agccagcctg tggggttttt atttctgttg
     3961 tagagagttg agttcatgga atccctgacg ttacaaccca tcgctcgtgt cgatggcact
     4021 attaatctgc ccggttccaa gagcgtttct aaccgcgctt tattgcttgc ggcattagca
     4081 cacggcaaaa cagtattaac caatctgctg gatagcgacg acgtgcgcca tatgctgaat
     4141 gcattaacag cgttagggtt aagctatacg ctttcagccg atcgtacgcg ttgcgaaatt
     4201 atcggtaacg gcggtccatt acacgcagaa ggtgccctgg agttgttcct cggtaacgcc
     4261 ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg atagcaatga tattgtgctg
     4321 accggtgagc cgcgtatgaa agagcgcccg attggtcatc tggtggatgc gctgcgcctg
     4381 ggcggggcga agatcactta cctggaacaa gaaaattatc cgccgttgcg tttacagggc
     4441 ggctttactg gcggcaacgt tgacgttgat ggctccgttt ccagccaatt cctcaccgca
     4501 ctgttaatga ctgcgcctct tgcgccggaa gatacggtga ttcgtattaa aggcgatctg
     4561 gtttctaaac cttatatcga catcacactc aatctgatga agacgtttgg tgttgaaatt
     4621 gaaaatcagc actatcaaca atttgtcgta aaaggcgggc agtcttatca gtctccgggt
     4681 acttatttgg tcgaaggcga tgcatcttcg gcttcttact tcctggcagc agcagcaatc
     4741 aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca gtatgcaggg tgatattcgc
     4801 tttgctgatg tgctggaaaa aatgggcgcg accatttgct ggggcgatga ttatatttcc
     4861 tgcacgcgtg gtgaactgaa cgctattgat atggatatga accatattcc tgatgcggcg
     4921 atgaccattg ccacggcggc gttatttgca aaaggcacca ccacgctgcg caatatctat
     4981 aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg caacagaact gcgtaaagtc
     5041 ggcgcggaag tggaagaggg gcacgattac attcgtatca ctcctccgga aaaactgaac
     5101 tttgccgaga tcgcgacata caatgatcac cggatggcga tgtgtttctc gctggtggcg
     5161 ttgtcagata caccagtgac gattcttgat cccaaatgca cggccaaaac atttccggat
     5221 tatttcgagc agctggcgcg gattagccag gcagcctgaa tgaacaacgg gcaataaata
     5281 gccaaatctt tctttatcaa aacgtcggca cattg
//
LOCUS       DAECOD010000173         5165 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.173, whole genome shotgun sequence.
ACCESSION   DAECOD010000173 DAECOD010000000
VERSION     DAECOD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.173"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..222
                     /locus_tag="KKO15_003788"
     CDS             <1..222
                     /locus_tag="KKO15_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6362401.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="KKO15_003789"
     CDS             complement(394..723)
                     /locus_tag="KKO15_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBD6362402.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="KKO15_003790"
     CDS             complement(728..913)
                     /locus_tag="KKO15_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBD6362403.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="KKO15_003791"
     CDS             complement(910..3549)
                     /locus_tag="KKO15_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBD6362404.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="KKO15_003792"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="KKO15_003792"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD6362405.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5165
                     /gene="hslJ"
                     /locus_tag="KKO15_003793"
     CDS             4857..>5165
                     /gene="hslJ"
                     /locus_tag="KKO15_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBD6362406.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1306 a         1323 c         1205 g         1331 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattggccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataac
//
LOCUS       DAECOD010000174         5141 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.174, whole genome shotgun sequence.
ACCESSION   DAECOD010000174 DAECOD010000000
VERSION     DAECOD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.174"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..250
                     /locus_tag="KKO15_003794"
                     /pseudo
     CDS             1..250
                     /locus_tag="KKO15_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..1489
                     /locus_tag="KKO15_003795"
     CDS             743..1489
                     /locus_tag="KKO15_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD6362407.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1504..1926
                     /locus_tag="KKO15_003796"
     CDS             1504..1926
                     /locus_tag="KKO15_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD6362408.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1984..2340
                     /locus_tag="KKO15_003797"
     CDS             1984..2340
                     /locus_tag="KKO15_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362409.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2433..2651
                     /locus_tag="KKO15_003798"
     CDS             2433..2651
                     /locus_tag="KKO15_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD6362410.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2653..3018
                     /locus_tag="KKO15_003799"
     CDS             2653..3018
                     /locus_tag="KKO15_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6362411.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            3015..3680
                     /locus_tag="KKO15_003800"
     CDS             3015..3680
                     /locus_tag="KKO15_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362412.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3680..4045
                     /locus_tag="KKO15_003801"
     CDS             3680..4045
                     /locus_tag="KKO15_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBD6362413.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4500..4655
                     /gene="hokD"
                     /locus_tag="KKO15_003802"
     CDS             4500..4655
                     /gene="hokD"
                     /locus_tag="KKO15_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6362414.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1408 a         1086 c         1413 g         1234 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgc cagaaacact
      481 ctggcatgag tgggttcagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgct
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctga accgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
     1381 tctcggcgag cgggtgatgg atcgcatggt catgaacggc gggcgctggg ttaattttaa
     1441 ctgggagagc tggcgtccga atgttagcca ttcgagggtt gttaagtagt ttcaggagga
     1501 tttatggcga aaccttttac tcccgaacag cgggaagaac tgaagacgcg aattgtggaa
     1561 ctcgtgcatc aggacggtcg ggtcacgatt cggcagttgt ccgatgaaac aggtatcagt
     1621 cgtgcgtctg tcggtcgctt atgcatagaa ctggtcgcaa gtggtgatgt atataattct
     1681 ggctacggct tattcccgtc tgaacaggct cgcaaggact ggcaaagcgc ccgcaaaaaa
     1741 ctctcgagag taaaggtgag gaaaccggtt gttgttgatc cggaccttat ctggtcatta
     1801 cctgacggag aaatacgccg ctacgacagg cgcctgaata taatctgtcg cgagtgccgg
     1861 aagagcgaag ctatgcagcg tgtactggct ttctatcagg gtaattttca ggaggcgata
     1921 ctgtgaatga aattagctat caggcttcaa ttaccgctgg cattcgcatc aaaggagagg
     1981 agcatggaaa taaaaccaga agatgagtta agcaatatcg ttttatttcc gataaaagag
     2041 gatgaccctc gtaatcaggt taattttctt tataagccat cggaaagacc atattgccat
     2101 cacgcctctg tacgggttga cgaaaaagag cgtcaggtcc gctgtaaaat ctgcggtgcg
     2161 gttgtggagc cgtttgactg gatgctctct gtggcaaaaa gagaaaccag actggcagat
     2221 gatgtaaggc tattgcgcca ggaggaacag gaaaggcgga gaaatataga aaagctgata
     2281 cagattgagc gtaacgcgaa agcgcggata cgcagggcga caaaatccag aactgaataa
     2341 ttaaatttag cactgttaaa aatttaatcc ttaaccggag ggatttctgc accctcaaat
     2401 catcaggaga ccgcccgaaa gggcggtaat gaatggttac attatttaga aaaaaatatc
     2461 cgcgaaagag tagaacaaca gaattcctgt ttctcattct gtttatcgtg ttgatgacac
     2521 cgatatcccc gcaaattttt gtctggataa tcggaaaaat aattgagcca gttattgaat
     2581 tgtataacga cgtggtatgg gcgtcattca acacactgca caataaaatt aatccgtata
     2641 aggaaagctg atatggcact gacgaaaaaa caacgtgcag aactgcgcat gaagttcggt
     2701 ggtcgctgcg cttattgcgg ctgcgaactt ggcgaaaagt ggcatgcaga ccatgtaaaa
     2761 ccggtcattc gttttgatgg aaatatgctt caccaggaac gtgacgatat atccaacatg
     2821 gtaccagaat gccacccatg caatctgcac aagcattgca gtagcctgga agattatcga
     2881 cgaattatca gtgatggtcg tcgtgaattc cttgcgtccg ggaaaggcaa agcgctggtt
     2941 cgtatgggat tggttgaaat gaaatctgac ccggttgtgt tctggtttga aaaatatcaa
     3001 gaaggggcta cggcatgact actattacca aagagcgact gcaatggctg gctaacattt
     3061 ctggccgcga tgatattgac gatatagacg gcggtgaaat tcgtgaactg gcgcttatcg
     3121 ctctggcatc actggaagca gagcctgtag cggaatgcat tgttgaagat gggggcatgt
     3181 gtattgacgg gttcggtgag tatgtgggtc actcgctgcc tgatggaatg catgagcttt
     3241 atgctgcccc gcgaatgcga caacctgatg gatggaaagc cgtagctgta gcgtggaagg
     3301 tgacgtttac tcaagttgac caggaatcta atacgttcac tgctatatat ttttacaaag
     3361 cggaagttga gcgttgggta cgactgcata aagcatgtga ttttcgggca gatataacac
     3421 cgctttacgc agcgccggca gtgccggttg cagtaaacga cgacatggct tacgcattcc
     3481 atcacgcact gtcagattca tcgcttggct ctgatgaaat cgaagaaatt aaaaccggtt
     3541 tgcgtgctgc ctttgccaac gtcactatcc aaccagagcc gatagtgccg gatgaaatcg
     3601 ggccaaacga tagtaatacg tttgattatg ttgatggctg gaacgcctgc cgcgctgcca
     3661 tgcttaaggg agataaataa tgattaatcg aaccaaactg gagcacattc tcgaatatgc
     3721 caggcagcag aaatgtattg ggcaactttg taaaattcca ccaggagata tggttgaaat
     3781 cgtggaaatg gccatgcgta aggctggcaa ctctccggta actctggctg gctggataag
     3841 ctgtagtgat gcagttcctg ctgaatattg cgatgtgatt cttctcgatg atctcgggaa
     3901 tgtattcccc ggttcctggg ataaggtttt ttgccccact cgtggcggga ataagatggc
     3961 ttttgtggac aaagacggcg ttgaagtaga gagctcaact cactggatgc cgctaccgga
     4021 accaccgcag gaggtgaatc aatgacctgg cctgaggcat tcaccacggt aggaattgcg
     4081 atggcggtgg cgctggtggt gtattcgatt tgccgttggg gttaacaaac aaaaaccccg
     4141 gattgatggt ccggggtttt tgaaggaaac aaaacagaaa cagcaattgc cgttacctgt
     4201 tgttaccatg gcaagtaaac gtatctcagg cgagcgcatt gcgccgttct gacgcagata
     4261 aattagcctg gatagaaggt gctggcaata aaaaatagcg ttttcttatc ggtatcggta
     4321 agattgctgc gggtgcttga ggctgtctgc ctcgggcatg ccactgtaag gcagacagag
     4381 aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct gaggcccaat
     4441 ctatgtctca caaatgtagg ttagcctctt acgtgccgaa aggcaaggag aagcaggcta
     4501 tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga
     4561 cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc cagacggagg
     4621 tcgctgtctt cacagcttac gaacctgagg agtaagagac caggcggggg agaaatccct
     4681 cgccacctct catgtgtcag gcatcctcaa cgcacccgca ctaaacccgc tttggcgggt
     4741 tttttgttgc gtgctgaata cgcagggtga aaaataacca tatatttgat tatatacaca
     4801 acaaaaaata aaagtcattg tacttgcaca ttaaacaatc aaatatacgg cgtgaaataa
     4861 atatttatca gattaatatt tttgtctcta tgtggatata accgtttgta cttataaacc
     4921 tgggggcatc gtggaaaaaa taaagaaact atttagtagc aaatacgcag tcatacgtcg
     4981 tgatgacctg tcagttatag tcgaaatgga ttacttccct gaaaccccaa aatcaataat
     5041 gtatcgtaat ggtcgaaagg caattttttt accgatgagg gtaagtgaca ttatgggaaa
     5101 tgataaactg ctggatgaat tgcgagtcag agcatcctgt t
//
LOCUS       DAECOD010000175         4983 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.175, whole genome shotgun sequence.
ACCESSION   DAECOD010000175 DAECOD010000000
VERSION     DAECOD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.175"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..338)
                     /locus_tag="KKO15_003803"
     CDS             complement(<1..338)
                     /locus_tag="KKO15_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBD6362415.1"
                     /translation="MLPHFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLV"
     gene            complement(567..812)
                     /locus_tag="KKO15_003804"
                     /pseudo
     CDS             complement(567..812)
                     /locus_tag="KKO15_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            840..1187
                     /locus_tag="KKO15_003805"
                     /pseudo
     CDS             840..1187
                     /locus_tag="KKO15_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            1177..1971
                     /locus_tag="KKO15_003806"
     CDS             1177..1971
                     /locus_tag="KKO15_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6362416.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1993..3417
                     /gene="patD"
                     /locus_tag="KKO15_003807"
     CDS             1993..3417
                     /gene="patD"
                     /locus_tag="KKO15_003807"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBD6362417.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            3627..4436
                     /locus_tag="KKO15_003808"
     CDS             3627..4436
                     /locus_tag="KKO15_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBD6362418.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(4838..4933)
                     /gene="yncL"
                     /locus_tag="KKO15_003809"
     CDS             complement(4838..4933)
                     /gene="yncL"
                     /locus_tag="KKO15_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBD6362419.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         1260 a         1098 c         1297 g         1328 t
ORIGIN      
        1 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtag
       61 gggaccaggc tcagcagata ctgtatccac tgtgcaccag ccttcacccc gcagtgcggg
      121 caggagcggc ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa
      181 caacaccact gcgtgtagcc catcagtgtg gtcccgcagg ccatgatttt tgtcactgat
      241 tcaattacca ccggacggac tgccccttcc ggttgtttct ccagccagtt aagccagcgg
      301 tttccctgct gaaaaatatc ggcaaaatgg gggagcatca gaagggcggg gcgactccgt
      361 ccggccagtg aaccgtgcca cactccgggc agtacacgta taggaaattg aaaaaccaga
      421 cgataccgct gtccacgcct tatgccacgt gagcttttct gcttttccct ccactccggt
      481 gttttctctc tctgcatggt cgattcttat accgcaacag gctgtttttt atacttgcac
      541 cgattcccgg gcacacttag cctgtgtcag gccggcatat acctcagcag actgatatcc
      601 cgcgcgtttt tcttcagccc ctccacattc agccccgcaa tggcccggcg gtataccatc
      661 cggccgccac acagcacgca ttcgaacggg tcacgactca ggaactttga gaggcgggtt
      721 tacgtttacg gggtttagct gaacgggcca tataaccacc tgaaagacaa tgacattgcc
      781 tgtttttata acggtaattg cagaccatga caagccgcag ccgtcaggct gcctactcga
      841 tgatcctgcc cgttcaggcg gcgcttgagc gtttgccgcc gtcattgttg caggcgtcgg
      901 ctgatctcgg cgcacgtcca cgacaaacct ttcgctatgt ggtgctgccg ctggcaatcc
      961 cgggtattgc cgctggctct atctttacct tctcactcac actgggcgat tttatcgtcc
     1021 cgcagctggt tggtcctcca ggatatttta tcggcaatat ggtttattcc cagcaggggg
     1081 cgattggcaa tatgccgatg gcggcggcat tcaccctggt gccgattatt ctcatcgcac
     1141 tgtacctggc gttcgtgaaa cgtctgggag cgttcgatgc actctgaacg cgcaccgttt
     1201 ttcctcaaac tggcggcctg gggcggcgtt gttttcctac attttcccat cctgataatc
     1261 gccgcctatg cgtttaacac tgaagatgcg gcgtttagtt ttccaccgca gggcctgacg
     1321 ctgcgctggt ttagcgtggc agcacagcgt agtgatattc ttgatgccgt gacgctgtca
     1381 cttaaagtgg cggcgctggc gacattaatt gcgctggtgt tagggacgct ggcagctgcc
     1441 gcgctgtgga gacgagactt tttcggcaaa aacgccattt cgctgttatt gctgctgccc
     1501 attgcgctgc cgggcattgt cactggtctg gcgctattaa ccgcctttaa aaccatcaat
     1561 ctggagccgg gatttttcac catcgtggtc ggtcatgcga ctttttgcgt agtggtggtg
     1621 tttaacaatg tcatcgcccg ttttcgccgc acctcgtgga gtctggttga ggcgtcaatg
     1681 gatcttgggg ccaatggctg gcaaaccttc cgctacgtag tgttgccgaa tctcagttcg
     1741 gcgttactgg caggaggaat gctggcgttt gccttgtcgt tcgatgaaat catcgttacg
     1801 acctttacgg caggtcatga acgaacgtta ccgttgtggt tgctcaatca gcttgggcga
     1861 ccgcgtgatg taccggtaac caacgtggtg gcactgctgg ttatgttggt aacaaccttg
     1921 ccgatcctgg gggcctggtg gctaacccgc gaaggcgaca atggtcaata accactgata
     1981 caggaataag ctatgcaaca taagttactg attaacggag aacgggttag cggcgaaggg
     2041 gaaaaacagc ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggta
     2101 tccgcagagc aggtcgatgc tgctgtgcgt gcggcagatg cagcatttgc tgaatggggg
     2161 caaaccacac cgaaagcgcg tgcggaatgc ctgctgaaac tggcggatgt tatcgaagaa
     2221 aatggtcagg tttttgccga actggaatcc cgtaattgtg gcaaaccgct gcatagtgcg
     2281 ttcaatgatg aaatcccggc gattgtcgat gtttttcgct ttttcgcggg tgcggcgcgc
     2341 tgtctgaatg gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc
     2401 gatccgttgg gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggccgcg
     2461 tggaaacttg ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt
     2521 accccgctga ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggcaggcgtg
     2581 attaacatac tgtttggcag aggcaaaacg gtgggtgatc cgctgaccgg tcatcccaaa
     2641 gtgcggatgg tgtcgctgac gggctctatc gccaccggcg agcacatcat cagccatacc
     2701 gcgtcgtcca ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt
     2761 gatgatgcgg atattgaagc agtggtcgaa ggtgtacgta catttggcta ttacaatgct
     2821 ggacaggatt gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg
     2881 gtggaaaaac tgggtgctgc ggtggcaacg ttaaaatctg gtgcgccgga tgacgagtct
     2941 acggagcttg gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcagtagaa
     3001 gaggcgaaag cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat
     3061 ggctattact atgcgccgac gctgctggct ggcgcattac aggacgatgc catcgtgcaa
     3121 aaagaggtat ttggtccagt agtgagtgtt acgcccttcg acaacgaaga acaggtggtg
     3181 aactgggcga atgacagcca gtacggactt gcatcttcgg tatggacgaa agatgtgggc
     3241 agggcgcatc gcgtcagcgc acggctgcaa tatggttgta cctgggtcaa tacccatttc
     3301 atgctggtaa gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg
     3361 tcactttatg ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagtt
     3421 agatgttagt ggctgttata ggacggccac tatcgtttta ggttctgagt ttaatggcgg
     3481 ttaagtatta gaatgattac attaataact ggcgtaaaaa ttaggctaaa tgtttgatgg
     3541 cgatttttca tcctgtcaca gttgattagc ataaggcttt agagtatcgt tattaaacta
     3601 atactttata agtagaagta ttaacaatga aagttcaatt gtacaaattc acggaagata
     3661 aaaataaaac ccttaccttc agatggacga aaaaacactt tgagttttgt atggataata
     3721 aaatcttttt aaatcataag gggaagaagt cgtataaaga aaggaattta tttcttttct
     3781 caaaaggtga caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc
     3841 ctgtgaaaaa tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga
     3901 atatacgtat aggtcgattt tgtagtattg catcaaatgt aaaaataatg ggtggaaatc
     3961 atcctttgaa tcgttttact actcacatga tgacttataa cggtgaattt gataagtttg
     4021 ctatgagtga atttgaacgt agctggacat tgaaaccatt cattaccaaa cctgaaaatc
     4081 cgattatagg aaatgatgtc tggataggaa atgatgttgt attgaaaggt ggcatcgcaa
     4141 taggtgatgg tgctgttatt gctgccaatt ccgttgttac caaagatgtg cccccatatg
     4201 ccatagttgc aggagttcct gcaaaaataa tcagatttag gtttgattca aatgttattg
     4261 atgaactgtt gagaataaaa tggtggaatt ataactactc agatcttccg gataataata
     4321 agtgtgatga tataaattat tttgttgaag agatgaatcg attgatatca aatggaaata
     4381 ttcaagagag ggactataaa aaattcaatc tttctgaggt attcagaggc ctataattat
     4441 ctgttttgca tgataaatgg gcaagataac cagggcgggg tttgcaattt caatggggca
     4501 gcgtcatcaa ggaggttgca aacatcctgc catgcatata ctttacgcca tctttttata
     4561 tttacgttat tccttgaaag tataaagcat ttatagactc ccgcgcgtat aaagtattcg
     4621 tgatagaaaa atgtgaaaac aagattccgt ttgaatcagt aagctgtttg tatagacgac
     4681 agtaggggga tgttttctac tcatttcaat gtagatgcca gcaaaaagaa gtaaatatta
     4741 aggataatgt tgcaaacttg gagagtatga gaagatcttg cgtactgtca gagagttgat
     4801 ttctctgaca gtacgtgctg cgtgcaaaat cactacatca aataaaccag ccaaatctca
     4861 tagagataag agtaaacaaa ccaacagcag caaagacatt tatcagtact acagttctac
     4921 tggaaacatt catttttgcc accttttatc attgccccta tgtgtatagt acgggttaag
     4981 aaa
//
LOCUS       DAECOD010000176         4973 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.176, whole genome shotgun sequence.
ACCESSION   DAECOD010000176 DAECOD010000000
VERSION     DAECOD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.176"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KKO15_003810"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KKO15_003810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBD6362420.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KKO15_003811"
     CDS             1020..1703
                     /locus_tag="KKO15_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBD6362421.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KKO15_003812"
     CDS             1700..2161
                     /locus_tag="KKO15_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBD6362422.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KKO15_003813"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KKO15_003813"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD6362423.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KKO15_003814"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KKO15_003814"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD6362424.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KKO15_003815"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KKO15_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBD6362425.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KKO15_003816"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KKO15_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBD6362426.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1304 c         1240 g         1254 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAECOD010000177         4922 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.177, whole genome shotgun sequence.
ACCESSION   DAECOD010000177 DAECOD010000000
VERSION     DAECOD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.177"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            193..1137
                     /gene="leuO"
                     /locus_tag="KKO15_003817"
     CDS             193..1137
                     /gene="leuO"
                     /locus_tag="KKO15_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBD6362427.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1292..1336)
                     /locus_tag="KKO15_003818"
     CDS             complement(1292..1336)
                     /locus_tag="KKO15_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362428.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1455..3179
                     /gene="ilvI"
                     /locus_tag="KKO15_003819"
     CDS             1455..3179
                     /gene="ilvI"
                     /locus_tag="KKO15_003819"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBD6362429.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3182..3673
                     /gene="ilvN"
                     /locus_tag="KKO15_003820"
     CDS             3182..3673
                     /gene="ilvN"
                     /locus_tag="KKO15_003820"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD6362430.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3685..3771
                     /locus_tag="KKO15_003821"
     CDS             3685..3771
                     /locus_tag="KKO15_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBD6362431.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3854..4858
                     /gene="cra"
                     /locus_tag="KKO15_003822"
     CDS             3854..4858
                     /gene="cra"
                     /locus_tag="KKO15_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBD6362432.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1190 a         1128 c         1301 g         1303 t
ORIGIN      
        1 attatgctgt ggtaaatgac tcattccacg gcaatggatt ctgtttttat cagaacccgt
       61 atctttatgt tttccgaatt ttactcattt tgctttttct tattttatat gcatgataaa
      121 tcatattctt caggattatt tctctgcatt ccaataaggg aaagggagtt aagtgtgaca
      181 gtggagttaa gtatgccaga ggtacaaaca gatcattcag agacggcgga gttaagcaag
      241 ccacagctac gcatggtcga tctcaactta ttaaccgttt tcgatgccgt gatgcaggag
      301 caaaatatta cccgtgccgc tcatgttctg ggtatgtcgc aacctgcggt cagtaacgct
      361 gttgcacgcc tgaaggtgat gtttaatgac gagctttttg ttcgttatgg ccgtggtatt
      421 caaccgactg ctcgcgcatt tcaacttttt ggttcagttc gccaggcatt gcaactagta
      481 caaaatgaat tgcctggttc aggttttgaa cccgcgagca gtgaacgtgt atttcatctt
      541 tgtgtttgca gcccgttaga cagcattctg acctcgcaga tttataatca cattgagcag
      601 attgcgccaa atatacatgt tatgttcaag tcttcattaa atcagaacac tgaacatcag
      661 ctgcgttatc aggaaacgga gtttgtgatt agttatgaag acttccatcg tcctgaattt
      721 accagcgtgc cattatttaa agatgaaatg gtgctggtag ccagcaaaaa tcatccaaca
      781 attaaaggcc cgttactgaa acatgatgtt tataacgaac aacatgcggc ggtttcgctc
      841 gatcgtttcg cgtcatttag tcaaccttgg tatgacacgg tagataagca agccagtatc
      901 gcgtatcagg gcatggcaat gatgagcgta cttagcgtgg tgtcgcaaac gcatttggtc
      961 gctattgcgc cgcgttggct ggctgaagag ttcgctgaat ccttagaatt acaggtatta
     1021 ccgctgccgt taaaacaaaa cagcagaacc tgttatctct cttggcatga agctgccggg
     1081 cgcgataaag gccatcagtg gatggaagaa caattagtct caatttgcaa acgctaactg
     1141 aatgcagaat aggccagaca tgaatgtctg gtttattctg cattttttat tgaatgtaga
     1201 attttattct gaatgtgtgg actctctatt ttaggattaa ttaaaaaaat agagaaattg
     1261 ctgtaagttg tgggattcag ccgatttatt atcaatttaa tccttcgtaa tggaggattt
     1321 tatcgtttct tttcaccttt cctcctgttt attcttatta ccctgcgttt atgtctctgg
     1381 ctgccaattg cttaagcaag atcggacggt taatgtgttt tacacatttt ttccgtcaaa
     1441 cagtgaggca ggccatggag atgttgtctg gagccgagat ggtcgtccga tcgcttatcg
     1501 atcagggcgt taaacaagta ttcggttatc ccggaggcgc agtccttgat atttacgatg
     1561 cattgcatac cgtgggtggt attgatcatg tattagttcg tcatgagcag gcggcggtgc
     1621 atatggccga tggcctggcg cgtgcgaccg gggaagtcgg cgtcgtgctg gtaacgtcgg
     1681 gtccaggggc gaccaatgcg attaccggca tcgccaccgc ttatatggat tccattccat
     1741 tagttgtcct ttccgggcag gtagcgacct cgttgatagg ttacgatgcc tttcaggagt
     1801 gcgacatggt ggggatttcg cgaccggtgg ttaaacacag ttttctggtt aagcaaacgg
     1861 aagacattcc gcaggtgctg aaaaaggctt tctggctggc ggcaagtggt cgcccaggac
     1921 cagtggtcgt tgatttaccg aaagatattc ttaatccggc gaacaaatta ccctatgtct
     1981 gccctgagtc ggtcagtatg cgttcttaca atcccactac taccggacat aaagggcaaa
     2041 ttaagcgtgc tctgcaaacg ctggtagcgg caaaaaaacc ggttgtctac gtaggcggtg
     2101 gggcaatcat ggcgggctgc catcagcagt tgaaagaaac ggtggaggcg ttgaatctgc
     2161 ccgttgtttc ctcattgatg gggctggggg cgtttccggc aacgcatcgt caggcactgg
     2221 gcatgctggg aatgcacggt acctacgaag ccaatatgac gatgcataac gcggatgtga
     2281 ttttcgccgt cggggtacga tttgatgacc gaacgacgaa caatctggca aagtactgcc
     2341 caaatgccac tgttctgcat atcgatattg atcctacttc catttctaaa accgtgactg
     2401 cggatatccc gattgtgggg gatgctcgcc aggtcctcga acaaatgctt gaactcctgt
     2461 cgcaagaatc cgcccatcaa ccgctggatg agatccgcga ctggtggcag cagattgaac
     2521 agtggcgcgc tcttcagtgc ctgaaatatg acactcacag tgaaaagatt aaaccgcagg
     2581 cggtgatcga gactctttgg cggttgacta atggggacgc ttacgtgacg tccgatgtcg
     2641 ggcagcacca gatgtttgct gcgctttatt atccattcga caaaccgcgt cgctggatca
     2701 attccggcgg tctcggcacg atgggttttg gtttacctgc ggcactgggc gtcaaaatgg
     2761 cgttgccaga agaaaccgtg gtttgcgtca ctggcgacgg cagtattcag atgaacatcc
     2821 aggaactgtc taccgcgttg caatacgagt tgcccgtact ggtggtgaat ctcaataacc
     2881 gctatctggg gatggtgaaa cagtggcagg acatgatcta ttccggccgt cattcacaat
     2941 cttatatgca atcactaccc gatttcgtcc gtctggcgga agcctatggt cacgtcggga
     3001 tccagatctc tcatccgcat gagctggaaa gcaaacttag cgaggcgctg gaacaggtgc
     3061 gcaataatcg cctggtgttt gttgatgtta ccgtcgatgg cagtgagcac gtctacccga
     3121 tgcagattcg cgggggcgga atggatgaaa tgtggttaag caaaacggag agaacctgat
     3181 tatgcgccgg atattatcag tcttactcga aaatgaatca ggcgcgttat cccgcgtgat
     3241 tggccttttt tcccagcgag gctacaacat tgaaagcctg accgttgcgc caaccgacga
     3301 tccgacatta tcgcgtatga ccatccagac cgtgggcgat gaaaaagtac ttgagcagat
     3361 cgaaaagcaa ttacacaagc tggtcgatgt cttgcgcgtg agtgagttgg ggcagggcgc
     3421 gcatgttgag cgggaaatca tgctggtgaa aattcaggcc agcggttacg ggcgtgacga
     3481 agtgaaacgt aatacggaaa tattccgtgg gcaaattatc gatgtcaccc cctcgcttta
     3541 taccgttcaa ttagcaggca ccagcgataa gcttgatgca tttttagcat cgattcgcga
     3601 tgtggcgaaa attgtggaag ttgctcgctc tggtgtggtc ggactttcgc gcggcgataa
     3661 aataatgcgt tgagaatgat ctcagtgcgc aatttgcagc ccaacatgtc acgttgggct
     3721 ttttttgcga aatcagtggg aacctggaat aaaagcagtt gccgcagtta attttctgcg
     3781 cttagatgtt aatgaattta acccatacca gtacaatggc tatggttttt tacattttac
     3841 gcaaggggca attgtgaaac tggatgaaat cgctcggctg gcgggagtgt cgcggaccac
     3901 tgcaagctat gttattaacg gcaaagcgaa gcaataccgt gtgagcgaca aaaccgttga
     3961 aaaagtcatg gctgtggtgc gtgagcacaa ttaccacccg aacgccgtgg cagctgggct
     4021 tcgtgctgga cgcacacgtt ctattggtct tgtgatcccc gatctggaga acaccagcta
     4081 tacccgcatc gctaactatc ttgaacgcca ggcgcggcaa cggggttatc aactgctgat
     4141 tgcctgctca gaagatcagc cagacaacga aatgcgctgc attgagcacc ttttacagcg
     4201 tcaggttgat gccattattg tttcgacgtc gttgccacct gagcatcctt tttatcaacg
     4261 ctgggctaac gacccgttcc cgattgtcgc gctggaccgc gccctcgatc gtgaacattt
     4321 caccagcgtg gttggtgccg atcaggatga tgccgaaatg ctggcggaag agttacgtaa
     4381 gtttcccgcc gagacggtgc tttatcttgg cgcgctgccg gagctttctg tcagcttcct
     4441 gcgtgaacaa ggtttccgta ctgcttggaa agatgatccg cgcgaagtgc atttcctgta
     4501 tgccaacagc tatgagcggg aggcggctgc ccagttattc gaaaaatggc tggaaacgca
     4561 tccgatgccg caggcgctgt tcacaacgtc gtttgcgttg ttgcaaggag tgatggatgt
     4621 cacgctgcgt cgcgacggca aactgccttc tgacctggca attgccacct ttggcgataa
     4681 cgaactgctc gacttcttac agtgtccggt gctggcagtg gctcaacgtc accgcgatgt
     4741 cgcagagcgt gtgctggaga ttgtcctggc aagcctggac gaaccgcgta agccaaaacc
     4801 tggtttaacg cgcattaaac gtaatctcta tcgccgcggc gtgctcagcc gtagctaatc
     4861 cgcgaacaaa aatacgcgcc aggtgaattt ccctctggcg cgtagagtac gggactggac
     4921 at
//
LOCUS       DAECOD010000178         4921 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.178, whole genome shotgun sequence.
ACCESSION   DAECOD010000178 DAECOD010000000
VERSION     DAECOD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.178"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            186..1016
                     /locus_tag="KKO15_003823"
     CDS             186..1016
                     /locus_tag="KKO15_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD6362433.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1157..1930)
                     /gene="uxuR"
                     /locus_tag="KKO15_003824"
     CDS             complement(1157..1930)
                     /gene="uxuR"
                     /locus_tag="KKO15_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBD6362434.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2145..3605)
                     /locus_tag="KKO15_003825"
     CDS             complement(2145..3605)
                     /locus_tag="KKO15_003825"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBD6362435.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3686..4870)
                     /gene="uxuA"
                     /locus_tag="KKO15_003826"
     CDS             complement(3686..4870)
                     /gene="uxuA"
                     /locus_tag="KKO15_003826"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBD6362436.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
BASE COUNT         1184 a         1292 c         1206 g         1239 t
ORIGIN      
        1 tgcaaataat tttaaatgag cgttttttga tactttagag tgtttcctac cctttaatat
       61 cactgtgtag agttgatatc actacaacag aaacatgcat gtcatttgtt tacttctctt
      121 tttactacag aaaaatttat ctatttttct aaacatcagt taaatactca taaaaggtaa
      181 ctattatgtc tatgccatta agcaatgccg ctaataacca attcaataca aataatcact
      241 tccttcatca tccaaaagtc gacagtgagt taacacgtcg atatgaatat gcacgcctgg
      301 atacggaaaa tatctatctt ctgcctcttg ctcgtggcaa taaccataat tacgatggaa
      361 aaagcgttgt cgaaattaga aaacttaata ttggcaaaca gtcctggcca tttaattatg
      421 tgacagatac atgcagagaa ttcaatggta ttacaacaac aggacgtatg ctctatcgaa
      481 acttgaaaat cacctcagct ttagacgaaa tatatggtgg tatatgtaaa aaggcgcatg
      541 ctgctacaga gttagcagaa ggtttacggc taaatttatt tatgaagagc cctttcgatc
      601 ctgttgagga ctatacagtt catgaaataa cattaggccc tggttgcaat gttcctggtt
      661 atgccggaac cacgatagga tatatttcaa cccttccagc ttctcaggca aaaagatgga
      721 ccaatgagca gccaagaatt gatatctata ttgatcagat aataactgtc tcaggggtag
      781 caaattcttc cggattcgcc cttgccgcat tacttaatgc taatattgag atgggaaatg
      841 acccaatcat tggtatagaa gcctatcctg gtaccgcgga gatccattca aaaatgggat
      901 ataacgttat ccccggtgat gaagatgcgc cactcaaacg catgactctg caaccgtcat
      961 ctcttccaga actgtttgaa ttaaaaaatg gtgaatggaa ttatattggt aaatagccac
     1021 tgagaagaat gagtactaat attcaggtta tacagatatt taaacaaaac cctgcaattt
     1081 acaaagtaat actcattgcg acttcttaat ttaagaaagc gcaaggaacg tttacccttg
     1141 cgcttattat aataagtcag gcgtcgactt tatccagcgg ccatgaatca aagagatagc
     1201 cgtcaaaata aatatcgtca acgttcgaga attccagcag acgttgctta acgttttcca
     1261 gatgctgcca cattgccagc ttcgccgctc gggcatcttt tttgattaac gcggcgagga
     1321 tctgtttgtg atcgcccaac cactctttgc gatacaggct gtcatccaga tggctgtgca
     1381 actggatcca cattggattg ttttcccgcc actgccagga ctgacggaat aactccacca
     1441 gcatgctgtt atgcgttgct tcagcaatgg cgagatggaa ctgcatgtca ccgctttcgc
     1501 tgctacccgg cgcgctggaa gccagttcac gctcttccag ttgcaatgcc tgacgcattt
     1561 tgacgatatc ttcgcgggta gcctgcagtg cggcaaactc ggcgatgtta ctttccagta
     1621 actgacgcgc ctgtaacagc tcaaaaggac ccgcatcatt gcagacgttg gcatccggac
     1681 tgtctgtgtt gtggctgcct gagctatcaa gcacatagat accggcaccc cggcgcactt
     1741 ccaccagccc tttgatctcc agcatgatca gcgcttcacg taccaccgtc cgcgtgacat
     1801 caagcatttc tgcaatttca cgctccggcg gcagccgttc gccaggattg tacggcgtct
     1861 ttatgatcag atcgcggatc atcgccccga cttcctggta aggtctttgc gcagaggtga
     1921 cagatttcat ggtgttgtgt ccgttaggtg cggttaatca aatttttgcc ggatgcggcg
     1981 tgaacgcctt atccggccta caacgggcaa ttttacggcc cccgttaatc tcaaactata
     2041 ccattagcac tggaagttgg cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa
     2101 atatcagttt gattgaccag taagtctgta gggtaatcag ttagttacag cgcagccaca
     2161 cactcgcgcg caccgcgttc gcacagctgc tgatatgccg ccgtcaccgc gccaacaaag
     2221 tcggcattct gcggcagatc atcggcaaaa ataccgctca ggcccagcag cgctttcacg
     2281 cggtctgcgc cctgatactg cgcgttaatc ttctggaact cggccagcat cgggtcgacc
     2341 acgtcaatgg cattaccctg ctcatccacg ccctgggtgt aacccatcca gccagccacg
     2401 cccagcgcca ggtgacgcca gctgccgccg ttttgcaggt gcagacgcac cgggtccagt
     2461 agacgctgcg gtaacttctg gctgccgtcc atcgcaatct gccaggtacg gtgacgcaga
     2521 gacgggttgc tgaaacgctc gatcagcagc gtcgcatagg cgttcaggtc cgtaccttcc
     2581 ggcatcgaca gcgttggcgc ttgttcctgc atcatcaggg caaaggccgc tttgcgataa
     2641 tccgggttag tcatggtgtc ggcaatggtt tcatagccgc cgaggtaacc gaggtacgcc
     2701 aggaaagagt ggctgccgtt cagcatacgc agcttcatca tttcgaacgg cacaacgtct
     2761 gcaacgaact gtgtgcccac tttatcccag tccgggcgac cattaacgaa gttatcttca
     2821 atcacccact gacggaacgg ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg
     2881 tcagcaattt cctgtaaggt ttctggcgtc gccgccggaa cgatgcggtc aaccatggtg
     2941 cacgggaagg tgacgttctc ttcaatccat gccgctagct gcgggtcacg cgcctgagcc
     3001 agccccagta ccgcgacctt cgccacatgg ccgttttcac gcacgttatc gcaggacatc
     3061 accgtaaatg ctttcagccc tttttcacga cgcagacgca aggcttcgac gatgtaacca
     3121 atcgcggact tcggcgcagt cgggttttcc agatcgtgct tgatcagcgg gttattgaga
     3181 tccagttgac cgcttgccgc atcagcgcag tagccttttt ccgtgaccgt tagagagaca
     3241 atcgccgttt gcggacgcgc catcgcgttg agaatacctt cgcagccgtc gatttccgga
     3301 tgcagcgctt ctttcatcga accgataatt ttcagctcgg tgctttctgc gcctttttcc
     3361 gctacggtgt acagcagttg ctgttttttc aggttttcga tcagcatgcg gtcgttgcct
     3421 ggcatcaggt taacttcgca gatgccccag tcgctgtcgg tgctttccag cagatgatgg
     3481 gtatacagcg cctggtgcgc gcggtgaaac gccccgcaac cgagatgcac aatgcgtgat
     3541 tccagacgag aatgatccca tgacgggcgg gcaaccggca gattgctgtc aacaatagta
     3601 gtcatcgtaa actccagaat ccgtcttcag agagtgacgg caaggaagaa ggaaccggca
     3661 cggcgcgtag ccatgccggt gggtattaac ggctaaagaa agcgcgctgg atcgccagtt
     3721 cgacaccgcg aacttcggcc aggcctttca gacgaccaat tgcggagtaa ccagggttgg
     3781 ttttcttctt caggtcgtcc agcatctgat gaccgtggtc cggacgcatc gggatcaggt
     3841 cttctttgcc ttccgcttta cgacggtgtt cttcttcaac aatcgctttc accacttcgt
     3901 acatatcaac gtcaccgttc aggtgcgccg cttcgtggaa ggttttcggg ttatcttcac
     3961 gcatggtgga gcgcagatgg gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa
     4021 ccagatcgtt gtcagcacgc acgccgtagg aaccggtgca catggtgaaa ccgttcgcca
     4081 tgctgtttac ggtatcaacc atccactgca tatcttcaat ggtggaaaca atgcgcggca
     4141 agccgaggat cgggcgcggc ggatcgtccg ggtgaaccgc catacgcacg ccaacttctt
     4201 cagcaactgg aataatcgct ttcaggaaga cggcaaagtt ttcgcgcagt ttggctttgt
     4261 cgatatcttt gtacagctcc aggtgcttac ggaactggtc gagggtatac ccttcttccg
     4321 cgcccggcag accggcaatg atgttacgcg tcagacgcgc tttgtcttcg tcgctcatag
     4381 tggcgaagcg ttcagctgcc tgagcaattt cttcttcggt gtaatccgct tccgcgcctg
     4441 gacgtttcag gatatgcatt tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt
     4501 tggagccgtc tggcagcacg tattcgaggt cagtacgggt ccagtcgagc accggcatga
     4561 agttgtagca cacggtgcga atgccacact gcgccaggtt gcgcagagtc tgctgatagt
     4621 tagcgatcca ctgctcatag ttgccagtgt gggttttgat atcttcgtga attggtacgc
     4681 tttctacgac agaccacacc aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct
     4741 cttctacgga ccatacttcg ccgttcggga tatggtgcag cgcggtaacc acgccagttg
     4801 cgcccgcctg acggacatca gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg
     4861 tctgttccat gatttttcct ctttcacata gtgggttcca cagcaaagag acgcggaacc
     4921 t
//
LOCUS       DAECOD010000179         4870 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.179, whole genome shotgun sequence.
ACCESSION   DAECOD010000179 DAECOD010000000
VERSION     DAECOD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.179"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..526
                     /locus_tag="KKO15_003827"
     CDS             <1..526
                     /locus_tag="KKO15_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBD6362437.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            723..2162
                     /gene="aldA"
                     /locus_tag="KKO15_003828"
     CDS             723..2162
                     /gene="aldA"
                     /locus_tag="KKO15_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBD6362438.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2204..3205)
                     /gene="gap"
                     /locus_tag="KKO15_003829"
     CDS             complement(2204..3205)
                     /gene="gap"
                     /locus_tag="KKO15_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD6362439.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="KKO15_003830"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="KKO15_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBD6362440.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="KKO15_003831"
     CDS             4169..4342
                     /locus_tag="KKO15_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362441.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="KKO15_003832"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="KKO15_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBD6362442.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="KKO15_003833"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="KKO15_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1121 c         1207 g         1295 t
ORIGIN      
        1 caccactggc agagcagaag cgaccatcct ggtggatatc gctcatcagt tctggcatat
       61 tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa acgcacgctt
      121 tagcatcacg ctattgaatc aggccgtaga acgagttcat acggctatcg ttgtacagga
      181 ccccaccatg cagcggcgca cgatggcaac gttccgccgt atgaccgggg acaatcccga
      241 tgcaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata acgcagacag
      301 tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc tctcactact
      361 cactggcgag ctgccgcgtt tacacgatga tagcgatggc tacggtcccc gcgggcgaga
      421 ttttatcgtt cacgttgatt ttccggcaga agtcatccat gcatggcaaa cgctgaaaca
      481 tgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat tgcccgtttg
      541 tgaaccactt gtttgcaaac gggcatgact cctgactttt atttctgcct tttattcctt
      601 ttacacttgt ttttatgaag ccctccacag acttgtcctt tcccgatgcc gtctctctga
      661 tgattgatgt taattaacaa tatattcacc gaaaacaaac atataaatca caagagtcgc
      721 ccatgtcagt acccgttcaa catcctatgt atatcgatgg acagtttgtt acctggcgtg
      781 gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc cgcatacccg
      841 atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat
      901 gggaagcgtt gcctgctatt gaacgcgcca gttggttgtg caaaatctcc gccgggatcc
      961 gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag atccagcagc
     1021 tagctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg gagtgggcac
     1081 ggcgttacga gggcgagatt attcaaagcg atcgtccggg agaaaatatt cttctgttta
     1141 aacgtgcgct tggtgtgact accggcattc tgccgtggaa ctttccgttc ttcctcattg
     1201 cccgcaaaat ggctcccgct cttttgaccg gtaataccat cgtcattaaa cccagtgaat
     1261 ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc cttccgcgcg
     1321 gcgtgtttaa ccttgtactg gggcgtggtg aaaccgttgg gcaagaactg gcgggtaacc
     1381 caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag atcatggcga
     1441 ctgcggcgaa aaacatcacc aaagtgtgtc tggaattggg tggtaaagca ccggctatcg
     1501 taatggacga tgccgatctt gaactggcag tcaaagccat cgttgattca cgcgtcatta
     1561 atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc atttatgatc
     1621 agttcgtcaa tctgctgggt gaagcgttgc aggcggttca atttggtaac cccgctgaac
     1681 gcaacgacat tgcgatgggg ccgttgatta acgccgcggc gctggaaagg gttgagcaaa
     1741 aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa gcggtagagg
     1801 ggaaaggata ttattatccg ccgacattgc tgctggatgt tcgccaggaa atgtcgatta
     1861 tgcatgagga aacctttggc ccggtgctgc cagttgtcgc atttgacacg ctggaagatg
     1921 ctatctcaat ggctaatgac agtgattacg gcctgacctc atcaatctat acccaaaatc
     1981 tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg tgaaacttac atcaaccgtg
     2041 aaaacttcga agctatgcaa ggtttccacg ccggatggcg taaatccggt attggcggcg
     2101 cagatggtaa acatggcctg catgaatatc tgcagaccca ggtggtttat ttacagtctt
     2161 aatgagtgaa agaggcggag gttttttcct ccgcctgtgc gcgtcagagt ttagcgaatt
     2221 tttcgagggt gcgaataagc tgcgtgacga agccatattc gttatcgtac caggcgaccg
     2281 ttttcaccag ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca tcaaacactg
     2341 aaccgaaatg gctgccaatg atatcggaag agactatttc ttcatcggta taaccaaatg
     2401 actcgttatt ggtggttgct tgtttaagtg cgttattcac ctcttcggca gtcacttttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat cccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAECOD010000180         4862 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.180, whole genome shotgun sequence.
ACCESSION   DAECOD010000180 DAECOD010000000
VERSION     DAECOD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.180"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..426
                     /locus_tag="KKO15_003834"
     CDS             <1..426
                     /locus_tag="KKO15_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6362443.1"
                     /translation="NPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPL
                     GVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL
                     GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            760..3039
                     /gene="ydeP"
                     /locus_tag="KKO15_003835"
     CDS             760..3039
                     /gene="ydeP"
                     /locus_tag="KKO15_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBD6362444.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3287..3490
                     /gene="safA"
                     /locus_tag="KKO15_003836"
     CDS             3287..3490
                     /gene="safA"
                     /locus_tag="KKO15_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBD6362445.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3558..4319
                     /gene="ydeO"
                     /locus_tag="KKO15_003837"
     CDS             3558..4319
                     /gene="ydeO"
                     /locus_tag="KKO15_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBD6362446.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4721..>4862
                     /locus_tag="KKO15_003838"
     CDS             4721..>4862
                     /locus_tag="KKO15_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD6362447.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1390 a         1037 c         1079 g         1356 t
ORIGIN      
        1 aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat gccaacaggc
       61 ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc tggaagcgca
      121 gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta tctttcgggt
      181 gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc aacaaaagca
      241 tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc
      301 tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa
      361 acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat
      421 gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg cgcggataat
      481 tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat tgaagccaga
      541 gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat gatgcttaac
      601 ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc
      661 ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat
      721 tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc
      781 taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag
      841 cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc agagggcttt
      901 gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa
      961 aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt
     1021 gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga ggctgcgggg
     1081 cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg
     1141 caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt
     1201 gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc
     1261 cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc
     1321 gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag
     1381 aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg
     1441 acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag
     1501 gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct
     1561 gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc
     1621 aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc
     1681 tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt
     1741 gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa
     1801 atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg
     1861 gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa tttgctgttg
     1921 atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg tcactctaat
     1981 gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc
     2041 ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc aattgccagc
     2101 atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca
     2161 ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta
     2221 cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg
     2281 ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt
     2341 gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg
     2401 ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg
     2461 gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg
     2521 ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata
     2581 aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc
     2641 aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta
     2701 cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta
     2761 ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa
     2821 aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc
     2881 atggatagat taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt
     2941 ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc
     3001 tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg
     3061 ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt tgctcacatg
     3121 gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc
     3181 gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat attaagcata
     3241 ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac
     3301 agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg caattgtggt
     3361 tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat
     3421 ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata gtttgcaaga
     3481 ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat aaaactttat
     3541 tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat catgctttca
     3601 acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg atggtagata
     3661 acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc ggatttaatg
     3721 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc
     3781 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
     3841 atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc
     3901 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
     3961 tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
     4021 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
     4081 aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac
     4141 gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt
     4201 ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
     4261 gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat
     4321 tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc
     4381 cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac
     4441 atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg
     4501 tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg gcgagaaatt
     4561 acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc
     4621 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
     4681 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
     4741 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
     4801 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt
     4861 ta
//
LOCUS       DAECOD010000181         4832 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.181, whole genome shotgun sequence.
ACCESSION   DAECOD010000181 DAECOD010000000
VERSION     DAECOD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.181"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(286..1143)
                     /gene="sitD"
                     /locus_tag="KKO15_003839"
     CDS             complement(286..1143)
                     /gene="sitD"
                     /locus_tag="KKO15_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBD6362448.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1140..1997)
                     /gene="sitC"
                     /locus_tag="KKO15_003840"
     CDS             complement(1140..1997)
                     /gene="sitC"
                     /locus_tag="KKO15_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBD6362449.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1994..2821)
                     /gene="sitB"
                     /locus_tag="KKO15_003841"
     CDS             complement(1994..2821)
                     /gene="sitB"
                     /locus_tag="KKO15_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBD6362450.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2821..3735)
                     /gene="sitA"
                     /locus_tag="KKO15_003842"
     CDS             complement(2821..3735)
                     /gene="sitA"
                     /locus_tag="KKO15_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBD6362451.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4197..>4832)
                     /locus_tag="KKO15_003843"
     CDS             complement(4197..>4832)
                     /locus_tag="KKO15_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6362452.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1284 a         1177 c         1145 g         1226 t
ORIGIN      
        1 gatttatcgc cgtcagcggg agctgaagga agagctgaat agtctgaagg atttgaattc
       61 ggttaggaat tttatcgtgg aataacagaa gctgcggcac gtcgtatgca ggaacgtgcc
      121 gcggttggct ggtaaacttt cgatagtgcg agtattgaat gatttctagg cgttatcgat
      181 tttacgtatt ttttgcatga tagaattcgt acctcctccc aacgaccttc catggctttc
      241 cggacttctg taggctggcg gacgatgctg tcctcggatg ttgtgctaat tgggtgataa
      301 cagatcctgt gtatgggcgt ttccctttgt gcgtgcgttg atgctggtaa cggtaaacgt
      361 catgatgaac acgaccgcga ataagacgac aatcgtcgga gctggtgcgc tgtcgataaa
      421 aaaggacagc cagacaccag tcattgacac tattaccgat atgccagtcg ccagtaggag
      481 cgcaatatga aagcgttgtg tcaggagtac tgcaatggcg ccaggcgcaa tgagcagaga
      541 aatagagaga atgataccta ctgctttcag cgtcgccaca atggtcaggg aaaccatgca
      601 cagcagccca taatgcagcc atctcgtatg caaaccgctt acctgcgcct gttgataatc
      661 aaagctgaac agcaaaaaat cccgccattt tacgctaata acaagtgtaa ccagcccggc
      721 aataatcacc gtctggatta tatcgccgat ggttatcccg agcatgtcgc cgaaaagaat
      781 atggtcaaga tgtacgtctg gtttaattgc aatatacaag atcagacctg cggcaaacat
      841 gccagaaaaa acaatcccca taactgtatc ttgcttgatt cggctgttgt ctttcaggta
      901 tcccgtcgcc accgcgcaga atactccggc gacgaatgcc cctgtagcca gaggtaaccc
      961 cagaatccag gtaagcacga tgccaggaaa aaccgcatga ctcatcgcgt cacccatcag
     1021 tgcccaacct ttcaggacca gaaagaccga cagcagcgca cagggtatgg aaactaccag
     1081 tgaaatcagc agtgcgtgac tcataaaggt gaactgcagt ggctccagta ataatgccat
     1141 catgagcgat cctccagcgc tttatgcgca cgccgacgat ttgccaaaag tccatgggtg
     1201 ggcgcaaata caaatgctaa aagaaacagc agtgtctgcg ccaccacgat gattccacct
     1261 gtcgcgccgt ccagaaagta actgacccac gcgccgagga aactggttac actgccaata
     1321 gttacagcaa ttatgagcag gcggggaaaa cgatcggtta acaaccatgc tgttgcaccg
     1381 ggagtcacca ccagacagat caccagaaag gcaccaaccg tttgcagcgc cgcaacggtc
     1441 gaaaccgaca gcagggtgaa gaagaggatt tttaatctgc ccggatgtaa tccaatagca
     1501 cgggcgtgat tctcgtcgaa aaaggtcacc ataagatctt tccatttgaa aaaaagcacg
     1561 atgatcgaga gaatgccaat gatagtgagt tgcaggatat ccgccggatc gattgccaga
     1621 atattgccca ggacgatggt ctgaatgttc acggatgtcg ggttcaatga caccataaac
     1681 agccccaggc caaaaaagga cgagaagatc aggccgatga tggcatcttc ttttaagcgg
     1741 gtgcgttggt taagaaacag catgctgccc gctgccagtc cgccggaaaa gaaggctccc
     1801 agagaaaacg gaagccccag catatatgcc cccgcaacac ccgggacaat agagtgcgac
     1861 agcgcatcgc caatcagcga ccagcctttt agcatcagat agcatgacag gaaagcgcag
     1921 agaccgccga ccattgccga gacccacatc gcattgagca tatattcata gctgaagggt
     1981 tccagtagca cgttcatctt gcttccctct gcaccgctga cggtcgatgt gccacaaaag
     2041 ggcgttcatc gtcagtaata atgctttctt ccgagccgtt aagtgtgaca tggcgcagta
     2101 cgccgctaaa agccagctcc aggttctcgg ctgtaaaagt ggtgtctgtc ggcccgcttg
     2161 ccaggacggt gcctttgacc atgaccgtat aatcgcaaaa tgtcgtgact gagccgagat
     2221 tgtgcgttga gacaagcatc gttttgcctt ccgcgcgtaa ctccctcaac aggctgatga
     2281 ttttcgcttc ggttttcaca tcaacgccgg taaaaggttc atccagcagg atcacatcac
     2341 cctgctgtgc aattgctctc gccagaaaga cgcgtttctt ttgtccaccg gaaagctcgc
     2401 cgatttgacg atggcgaaaa tccaccatat cgacacgttc cagcgcatcg gtgacaatct
     2461 gacgatccct ctttttggct atacgcaaca tacccatatg cccatagcgg cccatcatca
     2521 ccacatcttc caccagtaca ggaaatgacc agtcaacctc ttctgactga gggacgtagg
     2581 caaccaggtt tttctgtagc gcctgtcgcg tgggaatacc cagaacagat atctttccgc
     2641 tggtcagacg cacaaatccc ataatcgctt taaacaacgt cgattttccg gaaccgttta
     2701 ccccaaccag agcggcaata gatccacttg gtactgtgaa ggatgcatcg cgcagtgctg
     2761 tgtgcccgtt acgccaggtg acagtaacat cattaacgac aatgcctgca gattgcatca
     2821 ttatttctcc cttttcccgg ctttaattcc ctgtaccagc gtactggtag taaccttaag
     2881 aaggtcgata tacgtaggta ccgggccgtt ttctgtgctc agggaatcga catagagcac
     2941 accgccgtag tgtgcgccgg tttcacgcgc aacctgacgc gctggtttat cggaaatcgt
     3001 actctcgcta aagactgccg ggatatgatt tttcttaact atatcaacaa ccttacgtac
     3061 ctgctgcggt gttccttgtt gatcggcatt aatcggccac agataaagct cttttagccc
     3121 caaatcgcgt gcgagataag aaaaagcccc ttcactggtg accatccatc gctgattctc
     3181 agggagttcc gtaatctgct tacgcagggg ggcaagggtt tgggtaatct tggctttata
     3241 agtatcggca ttacgttggt aggtttgtgc atttgccgga tcgtatttta tcaacgcatc
     3301 acgaatatta tcgacgtaaa tcagagcatt atctggcgac atccatgcat ggggattagg
     3361 tttgccctca tagggtcctt cggtgatccc tactggcgtc acacccgaag agacaattac
     3421 ttctggaacc ccattgagat gctggtaaaa gcgttggaac cacaattcca gattcatacc
     3481 attggcgaga atcagttgtg ccccctgcgc acgtttaata tcgccagggg taggctgata
     3541 ctcatgaatt tctgcaccag gcttggttat ggatgagact tctgcagcat ctccagccac
     3601 gtttttggcc atatccgcga tgatggtaaa tgttgtaatg accttgaatt tttcagttgc
     3661 acttgcctga aatgcgatcg agcaggtgag ggcgaggcac cccaagagca tggttacttt
     3721 ttttattgag tgcataattt agtacctata gtgagcaaaa ttagaacaaa catagcaacg
     3781 gctatgtttt atagcataac ctatacaaaa tgaaaataat tatcattaac agctttgagg
     3841 cctaaaacag ggattaggtt gtttcctgct ggcgacatga tattgaggaa aaaagtagag
     3901 cagctattag gctttgaaat gactttatga ggaaaattgt tgatggcaaa aatggggcaa
     3961 aacgctgtat aaggggcaaa aaaggggcaa aaaagagtgg gttatcgtag cttattgttg
     4021 tcgctgataa tgtttaacat attgaaaaat aaataaaata cttatgagtc agatagttgt
     4081 gatttttgcc cttacttgtt caggttgtat tgttctttct ttgtaatttg ttgattttct
     4141 tgcattattt cagttctctg gtactaaatg gggcaaattg ggggcaaact ttgcaactac
     4201 gataaccgcg cattcaacat ggctatctgt tcgtcgttca tgtcatcaat ccacataccg
     4261 taaatttcat acaccatttg cgcagtttca tgccccatct ggctggctat aaatgccggg
     4321 ttcgctcctg ccgtcaacag ccagcaggca aaagtatgtc gcgtgtggta cggattacgg
     4381 cggcgaatac cagcacgttt tactgctgca ttccatcttg tacccaaact gcttaccgag
     4441 taataaggtt tctgtttttc gttacacacc ctgggcatga aaacaaaatg cagtttttgt
     4501 ttttcagttc tgccgtactc ccgatgataa aaagtgattt cgcttttgcg atgatgcccg
     4561 gtcagtttgt attgctcctt cagtgcttca agagccggct gcagtaatgt taccgtccgg
     4621 atcccggcat ttgtttttgg gggaccgaac atatcaagta tcgtcaggtt tcttctgaca
     4681 ttcacaattc ccttctcgaa atccacatcc tcccacgcca gagctgccag ttccccgtga
     4741 cgaagcccgg agtaaacggc aaatttccac aagttttggc tctgtccttt ttcactttcc
     4801 attaatgcat tgaattctgt tttagataac gg
//
LOCUS       DAECOD010000182         4636 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.182, whole genome shotgun sequence.
ACCESSION   DAECOD010000182 DAECOD010000000
VERSION     DAECOD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.182"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..311
                     /locus_tag="KKO15_003844"
     CDS             <1..311
                     /locus_tag="KKO15_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD6362453.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KKO15_003845"
     CDS             468..1217
                     /locus_tag="KKO15_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBD6362454.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KKO15_003846"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KKO15_003846"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBD6362455.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KKO15_003847"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KKO15_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBD6362456.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KKO15_003848"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KKO15_003848"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBD6362457.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KKO15_003849"
     CDS             4084..4557
                     /locus_tag="KKO15_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBD6362458.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1163 c         1138 g         1097 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAECOD010000183         4575 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.183, whole genome shotgun sequence.
ACCESSION   DAECOD010000183 DAECOD010000000
VERSION     DAECOD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.183"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..357
                     /locus_tag="KKO15_003850"
     CDS             <1..357
                     /locus_tag="KKO15_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD6362459.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="KKO15_003851"
     CDS             619..981
                     /locus_tag="KKO15_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD6362460.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1898
                     /locus_tag="KKO15_003852"
     CDS             978..1898
                     /locus_tag="KKO15_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD6362461.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1901..2852
                     /locus_tag="KKO15_003853"
                     /pseudo
     CDS             1901..2852
                     /locus_tag="KKO15_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2879..3119
                     /locus_tag="KKO15_003854"
                     /pseudo
     CDS             2879..3119
                     /locus_tag="KKO15_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3116..3421
                     /locus_tag="KKO15_003855"
     CDS             3116..3421
                     /locus_tag="KKO15_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362462.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3655..4095)
                     /locus_tag="KKO15_003856"
     CDS             complement(3655..4095)
                     /locus_tag="KKO15_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBD6362463.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(4067..>4575)
                     /locus_tag="KKO15_003857"
     CDS             complement(4067..>4575)
                     /locus_tag="KKO15_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362464.1"
                     /translation="SVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKT
                     ALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNV
                     SSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSAD
                     AYYIAMGY"
BASE COUNT         1307 a          939 c          900 g         1429 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct ataccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatc gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaga
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtcagac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggtgaat atataggtag aatttactta gaaacaaaat
     1861 caagacctaa gtatattttg aagaaaggta gaaaataacc atgagcatat gtattaaaca
     1921 atcagcatta aaaatattat tggctttatc agcactccta ataacttggt tgactaccag
     1981 atactttcca gttgaaccgg atgttgctaa cagcccaatt gtatggaggc atattttaga
     2041 gaatggtata tcatcaatcc acgactggaa accaaccgtg gataattggt atttcaccgt
     2101 ttacccaatt aactttttat tttatatgct gttaggtgat gatggcttag tagcattaag
     2161 attatcaact gcagttttct ctattgctat agtaatagca gcgatgttaa cgctacgtaa
     2221 ggcattcggg tttacccctg ctttattttc tattatactt ctatctctaa taccatattt
     2281 ttcatacact tatggttttg tatcacatcc tttttctcat aattcaacaa acgctttcgg
     2341 ctttttatgc ttgcttatat ctgttttcaa catacagtat aaaaatatat ttatcactct
     2401 cctcttaagt ttgacagcat tattttccag cgtatccgat ccgtggttta cagctgcatt
     2461 ttttattcca ctattaatat cttacctttt attctctgta tgggataaaa aattatttaa
     2521 gcatactgca ttaatactat ttgcatgttt aatctctctt tccaatgtat tgcaaaatct
     2581 acttaacata cctccccacc aatttgaaat tgtatcactc aatgatatga ttttaaatgc
     2641 aaaatggtgc attctgttaa taggaaaaag tcttaattta ttagttgtcg acaataacgc
     2701 aacatcttac gcctcatttg tcatttggtt cattgccatt attacatctg cgtggtttgt
     2761 tttatcggac aataaaaaaa acacataccg tatttatatt gtgttatttt cattattatc
     2821 tatcgcaggt attgtctcat cattcatact gagctataaa tcccctgatt atattagcat
     2881 gcgtttcttt atgaatgtta cctgtttcgc actgatattg tgctgtatcg gtacatcaac
     2941 aaaagcaaag atattatttt atctaatcgc atttttattt tcgatcagtt caattaaatc
     3001 ttacacaaat aatgcttcac cattacacga tcaggagaag attgttaaat catacattga
     3061 ttttttaaaa aaaaataatc tccattatgg gtacggctct ttttgggatt tgtcaatgac
     3121 tgtaaattgg ttatctggtg gggacataca gataactcct gttttcttta atgctgattc
     3181 ggggaaaatt aactttactg gcgtgagaca acagacactg gcttcttggc atagcaagga
     3241 ggcgttcaat tctgctcctg agcgacagtt tatagcagtt tccatagcta acgaacctga
     3301 acgatgtaaa gaaatgacat cctgccttgc aggtattcaa gaacaattag gtaagcctga
     3361 cgaagttttg aactttgaag gaagagtaat attagtattt aacaaaaaac ttaacctata
     3421 gtaaattgcc ccctaccata taaaccacac taacgaatga ataacccgtc tttttcgacg
     3481 ggttatatcc ctatcaggaa taaaaatgga gtggttgccg tatacaacaa accctcattt
     3541 agagctatat tctagagtat atgagatact ataaagttga aggagctatc attagaaaaa
     3601 ccatatgctg ttataattag atgtctcgct tttcatccaa taacgaattt tctcttatag
     3661 attgctctct ggttttttcg gccactctgt attttttata tcaacacgca tcaatagtgc
     3721 gcgatatttt ttccatgcca gtaacagttt cgtttcatca tctgttgcca tatctaaatc
     3781 aaccgcatcc tgaagcggcg ctattacatt cgttgcctct gctatcagtg cagatttctt
     3841 ttcattattt attaaggcta attgttctgg tgttggagaa ggacggtcaa caatgacagg
     3901 gtgaccattc tcaccacagg taatcatttt tgttacactc tgcgaattaa tcagtgtttt
     3961 ccactctgat tccgagattt ccactgcatc atcaggaata ttggtgccgt gaaattcagt
     4021 cgaataaaat ccgtttgtcg atgcagaata aaaatatgac atttcgttaa tatcccattg
     4081 caatgtaata cgcatcagca gatgaaccat atcctgaacc gggcgacact gtaatatcaa
     4141 atccagtgtt ctggacattt gtcgccgata cagtcggctg ccctgacgcg ccagtaacgt
     4201 tcaatgtaac tgtgacagca aggactcttg agaatgtcat cgggaacctc atcccgtatg
     4261 ttgaagatac attaggggag ccattcaact gaccgcactg taaaattaat ccagacggaa
     4321 atttttgcgc agcagccgtt gcggtattac ttgacgcgaa caggctcata tcaggtatct
     4381 gattcgcccc tgtacctaca ttccttttag ctgcttctcc caaaccaagg ttttcgagag
     4441 ccgttttcac cgtaccatcc gatttgatat cgccaaacgg attcttgcgg cttaacagca
     4501 gcgcgcgaag tgcggtaagc aactgatcgt gccgcccctt ctccaggctg gcaccggatg
     4561 cctccaccac gctgc
//
LOCUS       DAECOD010000184         4550 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.184, whole genome shotgun sequence.
ACCESSION   DAECOD010000184 DAECOD010000000
VERSION     DAECOD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.184"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1224
                     /locus_tag="KKO15_003858"
     CDS             <1..1224
                     /locus_tag="KKO15_003858"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD6362465.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="KKO15_003859"
     CDS             1224..1898
                     /locus_tag="KKO15_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6362466.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="KKO15_003860"
     CDS             2426..3187
                     /locus_tag="KKO15_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD6362467.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="KKO15_003861"
     CDS             3231..4310
                     /locus_tag="KKO15_003861"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6362468.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="KKO15_003862"
     CDS             4307..>4550
                     /locus_tag="KKO15_003862"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD6362469.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAECOD010000185         4470 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.185, whole genome shotgun sequence.
ACCESSION   DAECOD010000185 DAECOD010000000
VERSION     DAECOD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.185"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            185..1042
                     /gene="focA"
                     /locus_tag="KKO15_003863"
     CDS             185..1042
                     /gene="focA"
                     /locus_tag="KKO15_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBD6362470.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1097..3379
                     /gene="pflB"
                     /locus_tag="KKO15_003864"
     CDS             1097..3379
                     /gene="pflB"
                     /locus_tag="KKO15_003864"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD6362471.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3571..4311
                     /gene="pflA"
                     /locus_tag="KKO15_003865"
     CDS             3571..4311
                     /gene="pflA"
                     /locus_tag="KKO15_003865"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBD6362472.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         1138 a         1104 c         1080 g         1148 t
ORIGIN      
        1 ttcaactcat ttgttaattt ttaaaattta tttttatttg gataatcaaa tagttactcc
       61 gtatttgcat aaaaaccatg cgagttacgg gcctataagc caggcgagat atgatctata
      121 tcaatttctc atctataatg ctttgttagt atctcgtcgc cgacttaata aagagagagt
      181 tagtgtgaaa gctgacaacc cttttgatct tttacttcct gctgcaatgg ccaaagtggc
      241 cgaagaggcg ggtgtctata aagcaacgaa acatccgctt aagactttct atctggcgat
      301 taccgccggt gttttcatct caatcgcatt cgtcttctat atcacagcaa ccactggcac
      361 aggcacaatg cccttcggca tggcaaaact ggttggcggc atttgcttct ctctggggct
      421 gattctttgt gttgtctgcg gagccgatct ctttacttcc acagtgttga ttgttgttgc
      481 taaggcgagt gggcgcatca cctggtgtca gttggctaaa aactggctaa atgtctattt
      541 tggcaacctg gtcggcgcac tgctgtttgt acttttaatg tggctttccg gcgagtatat
      601 gaccgcaaat ggtcaatggg gactaaacgt cctacaaacc gccgaccaca aagtgcacca
      661 tacttttatt gaggccgtct gtcttggtat cctggcaaac ctgatggtat gtctggcagt
      721 atggatgagt tattctggcc gcagcctgat ggacaaagcg ttcattatgg tgctgccggt
      781 cgcgatgttt gttgccagcg gttttgagca cagtatcgca aacatgttta tgatcccgat
      841 gggtattgta atccgcgact tcgcatctcc ggaattctgg accgctgtcg gttctgcacc
      901 ggaaaatttt tctcacctga ccgtgatgaa cttcatcact gataacctga tcccggttac
      961 gatcggtaat attatcggcg gtggtttgtt ggttgggttg acatactggg tcatttacct
     1021 gcgtgaaaac gatcaccatt aatggttgtc gaagtacgca gtaaataaaa aatccactta
     1081 agaaggtagg tgttacatgt ccgagcttaa tgaaaagtta gccacagcct gggaaggttt
     1141 taccaaaggt gactggcaga atgaagtaaa cgtccgtgac ttcattcaga aaaactacac
     1201 tccgtacgag ggtgacgagt ccttcctggc tggcgctact gaagcgacca ccaccctgtg
     1261 ggacaaagta atggaaggtg ttaaactgga aaaccgcact cacgcgccag ttgattttga
     1321 caccgctgtt gcttccacca tcacctctca cgacgctggc tacatcaata aagcgttgga
     1381 aaaagttgtt ggtctgcaga ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg
     1441 tatcaaaatg atcgaaggtt cctgcaaagc gtacaaccgc gaactggacc cgatgatcaa
     1501 aaaaatcttc actgaatacc gtaaaactca caaccagggc gtgttcgacg tttacactcc
     1561 ggacatcctg cgttgccgta aatccggtgt tctgaccggt ctgccagatg cttatggccg
     1621 tggccgtatc atcggtgact accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa
     1681 agacaaatac gctcagttca cctctctaca ggctgatctg gaaaacggcg taaacctgga
     1741 acagactatc cgtctgcgcg aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa
     1801 agaaatggct gcgaaatacg gctacgacat ctctggtccg gctaccaacg ctcaggaagc
     1861 tatccagtgg acttacttcg gctacctggc tgctgttaag tctcagaacg gtgctgcaat
     1921 gtccttcggt cgtacctcca ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg
     1981 caagatcacc gaacaagaag cgcaggaaat ggttgaccac ctggtcatga aactgcgtat
     2041 ggttcgcttc ctgcgtactc cggaatacga tgaactgttc tctggcgacc caatctgggc
     2101 aaccgaatct atcggtggta tgggcctcga tggtcgtacc ctggttacca aaaacagctt
     2161 ccgtttcctg aacaccctgt acactatggg gccgtctccg gaaccgaaca tgaccattct
     2221 gtggtctgaa aaactgccgc tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac
     2281 ctcttctctg cagtatgaga acgatgacct gatgcgtccg gacttcaaca acgatgacta
     2341 cgctatcgct tgctgcgtaa gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc
     2401 gcgtgcaaac ctggcgaaaa ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact
     2461 gaaaatgcag gttggtccga agtctgaacc gatcaaaggc gatctcctga gctacgatga
     2521 agtgatggag cgcatggatc acttcatgga ctggctggct aaacagtaca tcactgcact
     2581 gaacatcatc cactacatgc acgacaagta cagctacgaa gcctctctga tggcgctgca
     2641 cgaccgtgac gttatccgca ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga
     2701 ctccctgtct gcaatcaaat atgcgaaagt taaaccgatt cgtgacgaag acggtctggc
     2761 tatcgacttc gaaatcgaag gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga
     2821 tgacctggct gttgacctgg tagaacgttt catgaagaaa attcagaaac tgcacaccta
     2881 ccgtgacgct atcccgactc agtctgttct gaccatcact tctaacgttg tgtatggtaa
     2941 gaaaactggt aacaccccag acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa
     3001 cccgatgcac ggtcgtgacc agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact
     3061 gccgtttgct tacgctaaag atggtatctc ctacaccttc tctatcgttc cgaacgcact
     3121 gggtaaagac gacgaagttc gtaagaccaa cctggctggt ctgatggatg gttacttcca
     3181 ccacgaagca tccatcgaag gtggtcagca cctgaacgtt aacgtgatga accgtgaaat
     3241 gctgctcgac gcgatggaaa acccggaaaa atatccgcag ctgaccatcc gtgtatctgg
     3301 ctacgcagta cgtttcaact cgctgactaa agaacagcag caggacgtta ttactcgtac
     3361 cttcactcaa tctatgtaat tagatttgac tgaaatcgta cagtaaaaag cgtacaataa
     3421 aggctccacg aaagtggggc cttttttagc acgagagcct tttttgtcag ctatctatac
     3481 tttaaggtga ctgccaaaac agactcgacg tagccttcga gctgcgcacc aacacggcct
     3541 cagatgggcc acatctggag aaacaccgca atgtcagtta ttggtcgcat tcactccttt
     3601 gaatcctgtg gaaccgtaga cggcccaggt attcgcttta tcaccttttt ccagggctgc
     3661 ctgatgcgct gcctgtattg tcataaccgc gacacctggg acacgcatgg cggtaaagaa
     3721 gttaccgttg aagatttgat gaaggaagtg gtgacctatc gccactttat gaacgcttcc
     3781 ggcggcggcg ttaccgcatc cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac
     3841 tggttccgcg cctgtaaaaa agaaggcatt catacctgtc tggataccaa cggttttgtt
     3901 cgtcgttacg atccggtgat tgatgaactg ctggaagtaa ccgacctagt aatgctcgat
     3961 ctcaaacaga tgaacgacga gatccaccaa aatctggttg gagtttccaa ccaccgcacg
     4021 ctggagttcg ctaaatatct ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt
     4081 gtcccaggct ggtctgacga tgacgattcg gcacatcgcc tcggtgaatt tacccgtgat
     4141 atgggtaacg ttgagaaaat cgaacttctc ccctaccacg aactgggcaa acacaaatgg
     4201 gtggcgatgg gtgaagaata caaactcgat ggtgttaaac caccgaagaa agagaccatg
     4261 gaacgcgtga aaggcattct tgagcagtac ggtcataagg taatgttcta atttcctcgt
     4321 aaagcggcaa ctttattgag ttgccgcttt tttattccgc gcttcattct gctacagcgt
     4381 ttttaacaca tcttatagtg catctaccat atcccgtacc tgagataata acgactgata
     4441 atagctcgcg tgaagttctt ccccatcgaa
//
LOCUS       DAECOD010000186         4459 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.186, whole genome shotgun sequence.
ACCESSION   DAECOD010000186 DAECOD010000000
VERSION     DAECOD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.186"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(38..583)
                     /locus_tag="KKO15_003866"
                     /pseudo
     CDS             complement(38..583)
                     /locus_tag="KKO15_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1251..2372)
                     /locus_tag="KKO15_003867"
     CDS             complement(1251..2372)
                     /locus_tag="KKO15_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362473.1"
                     /translation="MALTNQINKASSLASLRQPQRDKDGQIIKGSVWGTDISRTDYVQ
                     MTNGQDAQVLNVGFTDRSGRPMAFGNDSHDYVQALEDSLDETFNDGDFRTAVMENVFP
                     CEFRPYGTDPTPIRRQALQNRTVRFKHNGAFNPAEDAPSTTVIKGASVTVTPVLNPET
                     NKTGILCSAATHVSDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTPDLQI
                     IEAAPLSGKPSDQAEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHE
                     GISELLGCTVCGYAGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLEL
                     ISVVDVLATGSVKVKAGEFDVEKDASFPLVHVIRFTTPE"
     gene            complement(2463..2873)
                     /locus_tag="KKO15_003868"
     CDS             complement(2463..2873)
                     /locus_tag="KKO15_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362474.1"
                     /translation="MSISYRKLDITLSADKETVLVFGQELSTKYFTEIVVTTMLNSTG
                     SDMAVSNRILNDIHAAGLDAGDYGKYSRWWAQSNAQERQEAERRRKEAKEHQERLAAI
                     HATPEEIAKAVAERKAREEALIKRFGNKGAAFGL"
     gene            complement(3065..3289)
                     /locus_tag="KKO15_003869"
     CDS             complement(3065..3289)
                     /locus_tag="KKO15_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362475.1"
                     /translation="MTNNTNDTNITSTASIKIDPRTPEGRKALRLMVIPTKALIATLG
                     LPAKENRQYYSKAALCLMAVDAGLTPRDFM"
     gene            complement(3296..4051)
                     /locus_tag="KKO15_003870"
     CDS             complement(3296..4051)
                     /locus_tag="KKO15_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD6362476.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIR
                     NMVEELNGSKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAG
                     YSVHLRAKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKANGLPAQKLMT
                     ILKKERKLFRVHPFSGEWSVNPNYVEYFRIIKPTDHRFNPNGINIRFNAKGLEFFSRP
                     ENVLKMHRKVIAVHGSDAAKQQHIQAVAKLEGR"
BASE COUNT         1152 a         1020 c         1054 g         1233 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacat
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctggg ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg aaccgaggta tcgagggtat
      841 cgaagataat cagccgctaa ccatttgttt ccttaaagga tgatgaatcc ttgtggttac
      901 agttatggtt acacattaat ttatctctgg tcaagtcctc tcgtgagtaa ccgaagtcct
      961 aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg ggaagtcgct
     1021 gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc caaggtcata
     1081 acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca atagcgagta
     1141 tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg accatataca
     1201 actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag ttactctggg
     1261 gtagtaaacc gaataacatg gaccaacggg aaagaggcgt ctttctcaac gtcaaactca
     1321 cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat taactcaagg
     1381 tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt agcgtcttta
     1441 gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc tgtatcatcg
     1501 cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg acctgcgcgt
     1561 tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc gtaatcgctc
     1621 agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag atcttcagct
     1681 tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc tggcgtatct
     1741 ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg catatcgcat
     1801 acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc agacacatga
     1861 gtagcagcag aacacaggat gcccgtctta ttggtctcag ggttcaacac tggggtgaca
     1921 gttacgcttg cccctttgat gactgtggta gacggagcat cctcagctgg attaaacgcg
     1981 ccattatgct taaagcggac ggtgcgattc tggagagcct gacggcgaat aggcgtaggg
     2041 tcagtgccgt atgggcgaaa ctcacaaggg aagacattct ccattacagc agtgcggaag
     2101 tctccatcat tgaaggtctc atccaaagag tcctccagcg cctgaacgta atcatgtgag
     2161 tcattcccaa aggccatcgg acggcctgag cggtccgtaa agcctacgtt gagaacttgg
     2221 gcgtcttgac cattggtcat ctgaacatag tcagtgcgtg agatgtcagt gccccataca
     2281 gaccctttaa tgatttgacc gtctttatct cgctgaggct gacgcagaga agccagtgag
     2341 gatgctttgt taatttgatt tgtaagagcc atgataaata tctccttata ctacgtaaaa
     2401 agtaactggt gacaccattg gtcttatctc agaatccaag cagcgccacc taaagtgatc
     2461 agttacagcc cgaaagcggc ccctttattg ccgaagcgtt taatcaatgc ttcctctcgg
     2521 gctttacgtt cagcgaccgc cttagcaatc tcttctggtg tcgcatgaat agcagccaga
     2581 cgttcttgat gttccttagc ttctttacgt cggcgttcag cctcttggcg ctcctgtgcg
     2641 ttgctttgag cccaccagcg ggaatacttg ccgtaatcac ctgcatcaag gccagcggcg
     2701 tgaatatcgt taagaatacg atttgagacc gccatgtcag agcctgtact gttcagcatg
     2761 gttgtaacga caatttcggt gaaatactta gtggatagct cctgaccgaa taccagtaca
     2821 gtttctttgt cagcactcag tgtgatatcc agtttgcgat aagaaataga catatttgtt
     2881 tctccttttg aaaagatgtt ttgaaagatt gaacttatcg gtcttccata ggtattccta
     2941 cggcgtctcc ccgcatctcg gtcagttcaa aaggatagct agtggtacta gtggtgctag
     3001 tggtgctagt ggtgctagtg gtgctagtgg tgctagtggt gctagtggtg ctagtattgg
     3061 ttaattacat gaaatctctt ggggtcagcc ctgcgtcaac cgccatgaga cagagggcag
     3121 ctttagagta atactggcgg ttttccttgg caggcagacc cagagtagct atcagtgcct
     3181 tggtgggtat caccatcagg cgtaacgcct tacggccttc tggtgtcctt gggtcaatct
     3241 tgatgctagc tgtgctagtg atgttggtgt cattagtgtt attagtcatg atgaattacc
     3301 tcccctccag ctttgctact gcctgtatgt gttgctgttt agcagcgtcg cttccatgaa
     3361 ccgctatgac ctttctgtgc atcttgagta cgttttcagg tcggctgaag aactccagcc
     3421 ccttagcgtt aaaccgaatg tttataccgt ttgggttgaa tcgatggtca gtaggtttga
     3481 taattcgaaa gtactccaca tagtttggat tgactgacca ctcgccactg aaaggatgta
     3541 ctcggaacaa cttgcgctct ttcttaagga tggtcatcag tttctgagcg ggtagaccat
     3601 tggccttgca gtaatcgccc caagtcacgc ctcgacgatt agctgactga atggctgctc
     3661 gtttcttctg gtcttccagt tgaagaactc gacgctctaa cgtctgaata tggtcgtaac
     3721 acttggcgcg taggtgaaca gaatagccag cgaccagaat aaaagtatgc ttacggtcga
     3781 gacgatacat tgttcggctc tcacctttcg cgtccttgta tacttcctcg ccaacgagcc
     3841 caaatttgga cccgttaagc tcgctgctca aagttgagca gtgctcagtt ttggacccgt
     3901 taagctcttc gaccatatta cgaatatctc gtaaaacgtg tttatgttct tttcctgtca
     3961 gctcagcaat ttcaactgag gtcatggaca caagctggtc gaggttaatg atgctgattt
     4021 cttgttgtgg ttgttgtggt tgttgagaca tcgcttgatt tcctttttgt ttgcaccctg
     4081 aagtcgcacc cccactgacc tactcaaagt ccccaagcga agggagacca gcaggacaac
     4141 aggatgcaaa cagaatgact ttgagattga atactatgat gatgacgatg tagctgtgcg
     4201 gagctactgg gagggtctct ctaggagcca ctctagggtc tctctagggt ctctctaggc
     4261 atcaggctat acgaaactac agggcatcct aaagaatgac gtacagggcc atagaggacc
     4321 atagagttag cttctggggg taatgcctag aggagaccct agagagaccc tagagagacc
     4381 atagagagac ctatagagag accctagaga agacctatag agaagaccta tagagagacc
     4441 ctagagaaga cctatagag
//
LOCUS       DAECOD010000187         4322 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.187, whole genome shotgun sequence.
ACCESSION   DAECOD010000187 DAECOD010000000
VERSION     DAECOD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.187"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..156)
                     /locus_tag="KKO15_003871"
     CDS             complement(<1..156)
                     /locus_tag="KKO15_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6362477.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(306..467)
                     /locus_tag="KKO15_003872"
     CDS             complement(306..467)
                     /locus_tag="KKO15_003872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362478.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(464..652)
                     /locus_tag="KKO15_003873"
     CDS             complement(464..652)
                     /locus_tag="KKO15_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD6362479.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            912..1247
                     /gene="iraM"
                     /locus_tag="KKO15_003874"
     CDS             912..1247
                     /gene="iraM"
                     /locus_tag="KKO15_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD6362480.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1528..1636)
                     /locus_tag="KKO15_003875"
                     /pseudo
     CDS             complement(1528..1636)
                     /locus_tag="KKO15_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2159..2235)
                     /locus_tag="KKO15_003876"
     tRNA            complement(2159..2235)
                     /locus_tag="KKO15_003876"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2199..2201),aa:Arg,seq:tct)
     gene            complement(2337..2412)
                     /locus_tag="KKO15_003877"
     tRNA            complement(2337..2412)
                     /locus_tag="KKO15_003877"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2377..2379),aa:Ile,seq:cat)
     gene            complement(2557..3378)
                     /locus_tag="KKO15_003878"
     CDS             complement(2557..3378)
                     /locus_tag="KKO15_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD6362481.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3393..3749)
                     /locus_tag="KKO15_003879"
     CDS             complement(3393..3749)
                     /locus_tag="KKO15_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD6362482.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3762..>4322)
                     /locus_tag="KKO15_003880"
     CDS             complement(3762..>4322)
                     /locus_tag="KKO15_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD6362483.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1187 a         1179 c          855 g         1101 t
ORIGIN      
        1 atatgtcagc agcccaaaca gcagactccc cagcacgcct attgccgccc actgagacgg
       61 ggaaaccctg tccagcaact gcaggaacca gtagcccgtt cccaccgctg acgtggtgta
      121 tgacacacct gttgtgattt tttccatctg gtccataccc cgtctcccgt tatccggaag
      181 ctgacaacaa taaaaaagcc accagttaac tactgatggc tctgataact catgcaagcg
      241 tctcagacga cccactgaca ctaccggtga gtttaacgat accttccatt tgactggctc
      301 actttttatg atgatgccgg tgcatttatc tccagcacca gactttctat ctcaacgcca
      361 tacgttgcat ttttggtaat atccgtcagc gtcagtgcat ttagtcccac tgccagactg
      421 tcttttatgg cctggaatgc cgggccagta cgatgacgta gtatcactcc ggctcagttg
      481 caccactgac caccacatca ccttctgctg caatcgcctg catcagggta taaggggtta
      541 tggccaccgg actaccaaac ggctgccagc cctctttcag tttatgtgtc agcttttccg
      601 caagatctga cggcggcgcc gccctgacaa catcatagtg tttaaatgcc atggttcttt
      661 ccaacatctg aaaaataatt ctttaaaata cctgacatgt aatacagaaa aaacacaaaa
      721 ccatacctta aataaaaacc tgattatcaa gcagatatgc atggataaac tacaagacga
      781 gatataaacc accctgcatt taaataaaca ataaacaaca tcagaaaaat aattctgctc
      841 tatggtttaa ttcaaaaata tcatttatac ttttcagaac atcaccagca aggcataaac
      901 aaggaaacta aatgaagtgg attgtgattg atacagttat ccagccatca tgcggaatat
      961 ctttttcagt catatggagt aaaataaaat taataatctg gtatcaatcg gatgctttct
     1021 tacctcctga aagtatattt acactgactc acacaggcat catgctcaat aacaaagtgc
     1081 tacctgtaac catttacaac gtagtaccat tcaataaaac attctggaat ttaatcaaaa
     1141 acagccagga atgccctaca aatacagata acgtattgaa tgaatgcttt aataaccgtt
     1201 gcactctgca aatatgtcct tatggactaa aacaacaaag tccataagga gtttactcac
     1261 atctgacaaa atcaatataa acagcccctc cggagagggg ctggagagtg gcgctatgtg
     1321 ccattccatg gtgccgggtg cctcccggtg aattcagtac cagcacctga atccgcgatt
     1381 atcccatata cctactcgct gattgcccct ccgcacaggg ggattcacca tgccagtttc
     1441 ttttaacaaa ctccccgcaa accagacaac agtcaaccgc ctgaattgtg aagtatttaa
     1501 aaatttctcc cgctaactga tacccggcta acagtctggc gttttctttt tcagcaacgg
     1561 gaaagcaaca accaccacac ccgccaccag cacaccgtca gccggcactg acattatccg
     1621 gctgctgcaa tgccattaac aaaaacagta agcaatcact ttttaccgta acaggtgata
     1681 atccagatat gtatctaccc cagatgagta atccgaagtt catccatacc acaggtcctg
     1741 gctattctgt tgtactcctg aacaagagca aataattctg aattagcaac catgaactca
     1801 tcgcaaaccc tctgtatagc atcactattc agaataataa cgtctcttcc cgaaagacga
     1861 tcaggagtac agaacaaaac tgtcaaacgg ctgaaggcct ttgctcgtgc tgcattgact
     1921 atatcaatac gctgcctaag gatgaaacac cccgacgcct catcaatatt cactctaccc
     1981 acaccatatg aatgataaat acttaatgct gaaaaaacca ttagaccgta taacaaatac
     2041 tcaatcaata cttaacagaa cttttatttt tgacaaatat ataatatttt caacaatatc
     2101 ctgagccagg tatatttcag tataaggctc tgccggaagg aatctggaag aatgaatatg
     2161 gcgcgctgta ctggattcga accagtgacc gattgcttag aaggcaattg ctctgtccgg
     2221 ctgagctaac aacgctgaat accgataatg gaccgccatc ggggacccgc cccccgcacc
     2281 aacaaccctg ttatcgtgtc gtctgctctt cctgataagc taatggcggt ttgtgatggt
     2341 ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg
     2401 agctaaaggg ccgggagcag aataataacg gtccgtaatt aatttcgcaa taaaaaaacc
     2461 cgctcagcgg cgggttgtag aaactcttct aacgccaggc ataaaacgcc catcatcatg
     2521 acgaatttac cacagattcc ggaaaaatca accttgttac ctagttacct tttttaactg
     2581 ccgctcagcc catgcttctt caatatcaaa ccgggtcacc agcgcatcat aaaatttctt
     2641 aactgttttt tcccatgacg cgcgtgttat ctggtttgtc acctcgcata tagcattaaa
     2701 tgcctccgtt gatggtagtc tttcatagcc acgaccacca caacgctggc agtctctgat
     2761 aacaggcata ccacgtttta ccgactcttc acgatgaatg gcgacaccac gcccacggca
     2821 atccttacag gcggtggaaa cctcaccctt tccgccacac tccggacagg caacttttac
     2881 cacctccctg acttttttcc attcttccca gtaagacgga tacacacctt tcgtacactt
     2941 tgcccatacc ggcggcttac catccggata ctggatcttg tttgtaaaaa ctatgctttc
     3001 aataaatttt tccccatagc aacaagggca ctgcttttta ctcgctgcgc tgcgggcata
     3061 atcctcaaaa gcgtacgaag ccataatgcg catcactacc ggttttattt ctgccggaag
     3121 ttttctcaac gccgccacac gatcgcaccg actgagtgca taatctgcca gtaattctgt
     3181 tgcccgcgcc ctgtcattca tactgatgcc cattttcccc aggaacgcag aaaaacccat
     3241 ctcagcccga ttctgtgtca tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc
     3301 ttttgacgtt gaggccgatg catcggtcag gccaggggat tttggggagt agtatttcgg
     3361 taaatcttcc agtttcattt tttgacctgc tcttaatgca ttatggggta aatcttcacc
     3421 cccagacgtc caccagatac tggctgacca cgaacgatat tgatttcatc aaactgctca
     3481 tcgtccatta acactcccgc atgcgtcagc gcatccagcg gtgctttcag gatattgtcc
     3541 aggtcgcgac gacgcttatc cggtggctct gcaatcacct ttatcgccag ccttccggac
     3601 aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgcttt
     3661 ccctcctccg agataaaata tgtgctgcca cggcgtcgcc agtaagtgtt caccgtcggc
     3721 gggtaaggta aaaccaaatc tatgagcatc agtcacctct tttacccaag cacgccagtt
     3781 gcaaaggcgt gatcaagaaa acgaaaaatt aaatcaacct gagaaccatg cttttcttcg
     3841 aacgccagag gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa
     3901 atatcgtgag atttggtccc cattccgccc tgaccatgac caatcaggtg atggggatcg
     3961 tcggctggct taccacaaca cgcacacggc tgtgtcttca cccagcgttt gtatttctcg
     4021 ttaacccagc ggcgacgttt aggtcgtttc atgaaagatt ccggagactc aggatcaacg
     4081 gcaatgctga ccaccgtctt ttcctgttgt gggttctgtt gctggtgggc gtgaggcagc
     4141 ggcgcaagat tttttgtgcg ctgtttcagt atgctggtgg cggtctgctc tcccggtacg
     4201 atgtcgcttt cacggtacat tgagcggatt ttttccgcac gcaaccccag cgaacgacgt
     4261 aataccgctt ccggtagcgc gtccgccacc tgattgcgga ccgcccacca ggataattca
     4321 gc
//
LOCUS       DAECOD010000188         4301 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.188, whole genome shotgun sequence.
ACCESSION   DAECOD010000188 DAECOD010000000
VERSION     DAECOD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.188"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..301)
                     /gene="tuf"
                     /locus_tag="KKO15_003881"
     CDS             complement(<1..301)
                     /gene="tuf"
                     /locus_tag="KKO15_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD6362484.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMD"
     gene            complement(416..491)
                     /locus_tag="KKO15_003882"
     tRNA            complement(416..491)
                     /locus_tag="KKO15_003882"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Thr,seq:ggt)
     gene            complement(498..572)
                     /locus_tag="KKO15_003883"
     tRNA            complement(498..572)
                     /locus_tag="KKO15_003883"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(537..539),aa:Gly,seq:tcc)
     gene            complement(689..773)
                     /locus_tag="KKO15_003884"
     tRNA            complement(689..773)
                     /locus_tag="KKO15_003884"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Tyr,seq:gta)
     gene            complement(782..857)
                     /locus_tag="KKO15_003885"
     tRNA            complement(782..857)
                     /locus_tag="KKO15_003885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Thr,seq:tgt)
     gene            1219..2169
                     /gene="coaA"
                     /locus_tag="KKO15_003886"
                     /gene_synonym="panK"
     CDS             1219..2169
                     /gene="coaA"
                     /locus_tag="KKO15_003886"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBD6362485.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2198..3163)
                     /gene="birA"
                     /locus_tag="KKO15_003887"
     CDS             complement(2198..3163)
                     /gene="birA"
                     /locus_tag="KKO15_003887"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBD6362486.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3160..4188)
                     /gene="murB"
                     /locus_tag="KKO15_003888"
     CDS             complement(3160..4188)
                     /gene="murB"
                     /locus_tag="KKO15_003888"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBD6362487.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1103 a         1070 c          969 g         1159 t
ORIGIN      
        1 cgtccatctg agcagcaccg gtgatcatgt ttttaacata gtcggcgtgc cccgggcagt
       61 ctacgtgtgc gtagtgacgg gtcggggtgt cgtattcaac gtgagaagtg ttgatggtga
      121 taccacgagc tttttcttcc ggcgcgttat cgatctggtc gaatgcgcga gcagcaccgc
      181 cgtaggtttt agccagtacg gtagtgattg cagcggtcag cgttgtttta ccatggtcaa
      241 cgtggccgat agtaccgacg ttaacgtgcg gttttgtacg ttcaaacttt tctttagaca
      301 tcgattgtcc ctctaagaca cggataaatc ggtgatatca ccacatcaac caggcaacat
      361 gcccgacttg ttgaatgcaa taaacagaag gaaaaaacag ggaggagaaa agaagtggtg
      421 ctgataggca gattcgaact gccgacctca cccttaccaa gggtgcgctc taccaactga
      481 gctatatcag cacatcttgg agcgggcagc gggaatcgaa cccgcatcat cagcttggaa
      541 ggctgaggta atagccatta tacgatgccc gcatcctaga actcggctac ctgattttca
      601 ttctgcactg aatatcgaga gaagctctct ttattcgagc cggtaagtga acttatcgtc
      661 tcgggctacg ccatcgcgtg gccgaaattg gtggtggggg aaggattcga accttcgaag
      721 tctgtgacgg cagatttaca gtctgctccc tttggccgct cgggaacccc accggacttg
      781 atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag ttgctctacc
      841 tactgagcta agtcggcatc aagtagcgcg cattctatgg agacatgcga gttcatgcaa
      901 ctaaaaaatt gcataatttg ttttattggt cacattttat gctacacgat gaagaaacag
      961 ccgaacggag ggcagattag cacacttttt caacatcatt gcgctcaaca atgcgctcct
     1021 gctaaaccat aattcttttt atcagatgga atatctgtca cattgctttt caacgatagc
     1081 ttcctggcag agattttttc ttattattcc tccccatctg gtgttaccct cctgcccatt
     1141 aacccattca acagaactgt gacgcgccat ggcaaatatc gctttgccga tagagctatg
     1201 accgccagaa acatgcttat gagtataaaa gagcaaacgt taatgacgcc ttacctacag
     1261 tttgaccgca accagtgggc agctctgcgt gattccgtac ctatgacgtt atcggaagat
     1321 gagatcgccc gtctcaaagg tattaatgaa gatctctcgt tagaagaagt tgccgagatc
     1381 tatttacctt tgtcacgttt gctgaacttc tatataagct cgaatctgcg ccgtcaggca
     1441 gttctggaac agtttcttgg taccaacggg caacgcattc cttacattat cagtattgct
     1501 ggcagtgtcg cggtggggaa aagtacaacc gcccgtgtat tgcaggcgct attaagccgt
     1561 tggccggaac atcgtcgtgt tgaactgatc actacagatg gcttccttca ccctaatcag
     1621 gttctgaaag aacgtggtct gatgaagaag aaaggcttcc cggaatcgta tgatatgcat
     1681 cgcctggtga agtttgtttc cgatctcaaa tccggcgtgc caaacgttac agcccctgtt
     1741 tactcgcatc ttatttatga tgtgatcccg gatggagata aaactgttgt tcagcctgat
     1801 attttaattc ttgaagggtt aaatgtctta cagagcggga tggattatcc acacgatcca
     1861 catcatgtat ttgtttccga ttttgtcgat ttttcgatat atgttgatgc accggaagac
     1921 ttacttcaga cgtggtatat caaccgtttt ctgaaattcc gcgaaggggc ttttacagac
     1981 ccggattcct attttcataa ctacgcgaaa ttaactaaag aagaagcgat taagactgcc
     2041 atgacattgt ggaaagagat caactggctg aacttaaagc aaaatattct acctactcgt
     2101 gagcgcgcca gtttaatcct gacgaaaagt gctaatcatg tggtagaaga ggtcagacta
     2161 cgcaaataat ttgcagggga gcgaatactc ccctttctta tttttctgca ctacgcaggg
     2221 atatttcacc gcccatccag ggttttatta ttccatcctg ctcaagtaat aaagccccct
     2281 gtttgtctat tccgcgtgaa atgccaaata tttctttatc accaatgata agtttcactg
     2341 ggcgattaat aaaattatcc agcttttccc agcgcgacag ataaggtgcc aatccttctt
     2401 gttcgaagag ttccaacgca gcacgtaatt cacgtattag catggccgcc aacgtattac
     2461 gatcaagatt gatccccgct tcctgcagcg tgatccaccc ctgattaacg acactctctt
     2521 caacacggcg cattgccatg ttgatcccgg ctccaatgac tatttgcgcc gcatcgccag
     2581 ttttgccagt cagctccaca agaatgcctg ccagcttgcg atcctgcaga tagaggtcat
     2641 taggccattt gacacgaact ttatctgctc ccagctttcg taatacttcc gccatcacga
     2701 taccgataac cagacttaaa ccaatcgccg ccgccgggcc ttgttccaga cgccagaaca
     2761 tcgacaaata taagtttgcg ccaaaaggcg aaaaccattt ccgcccccgg cgaccacggc
     2821 cagcctgctg gtattctgca acacaggcat cgcccgattt aagctctccg atacgatcaa
     2881 gaaggaactg attcgtggag tcaataactg gcagcacggc tacactaccg ccatccagct
     2941 gacccaatat ctgttcagca ttaagtaact ggatgggctc aggcaggctg tatcctttac
     3001 ccggaacggt aaagacatca acgccccagt cacgcagtgt ctgaatgtgt ttattaatag
     3061 ccgcccggct cattcccagc gtttcaccca actgctcgcc agagtgaaat tcaccgttcg
     3121 ctaacagggc aatcaatttc agtggcacgg tgttatcctt catgaaattg tctccactgc
     3181 gctcacttca cctgatgcac caataaagcg gacttcaggc tcaagccaga cattaaattt
     3241 ttcacccact ttttgtctta catggtgtgc cagttgcacc acatcttcgc tttttgcatt
     3301 gtcttcatta atgagaacta acgcctgttg acggtgcacc gcagccccac ccatttgcat
     3361 cccttttagc tggcactgat caataagcca acctgctgcc agttttactg aaccacccgc
     3421 ctgggggtaa tttggtgctg ttggaaattg cgccagtaat gctttagccg tttcggcaga
     3481 tacaacaggg tttttgaaga aactaccggc attgccattc acttttggat cagggagttt
     3541 ggtggtgcgc atatgacaca ccgcattaaa tacttgctgt ggcgttactg ttgtaggatc
     3601 cagacgagtt aagtcaccat acgttagtac aggttgccac tcttttggca gacgcagacc
     3661 tacggctaca atggcgaagc gatcctggta ttcatgttta aaaatactgt cgcgatagcc
     3721 aaaacggcac tctttggcag ttaagcgcac ttgcttgcct gtcgccagtt caacacaatc
     3781 aacataagcg caaactcgct gtaattctac gccataagca ccaatattct ggataggtga
     3841 tgagccgaca caaccaggaa ttaatgccag attttccaga ccaggcatac cttcctgcaa
     3901 agtgtatttt accaggcgat gccagttttc tccggctcct acatgtaaat accacgcatc
     3961 aggttcatca tgaatttcga tacctttgat ccggttgatg atcaccgtgc cgcgatagtc
     4021 ttccagaaaa agtacattac ttccttcacc cagaataaga acgggttgtc cttctgcggt
     4081 tgcatgctgc caggcattga gtaattgttg ttcgtcttcg gcacatacaa tgtgctgagc
     4141 attatgatca atgccaaatg tgttccaggg ttttaaggag tggttcatag ctgctttcct
     4201 gatgcaaaaa cgaggctagt ttaccgtatc tgtgggggga tggcttgtag atatgacgac
     4261 aggaagagtt tgtagaaacg caaaaaggcc cgactgggct t
//
LOCUS       DAECOD010000189         4215 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.189, whole genome shotgun sequence.
ACCESSION   DAECOD010000189 DAECOD010000000
VERSION     DAECOD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.189"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(72..353)
                     /locus_tag="KKO15_003889"
     CDS             complement(72..353)
                     /locus_tag="KKO15_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBD6362488.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(615..1724)
                     /gene="apbC"
                     /locus_tag="KKO15_003890"
     CDS             complement(615..1724)
                     /gene="apbC"
                     /locus_tag="KKO15_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBD6362489.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1856..3889
                     /gene="metG"
                     /locus_tag="KKO15_003891"
     CDS             1856..3889
                     /gene="metG"
                     /locus_tag="KKO15_003891"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBD6362490.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4030..>4215
                     /locus_tag="KKO15_003892"
     CDS             4030..>4215
                     /locus_tag="KKO15_003892"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBD6362491.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
BASE COUNT         1079 a         1064 c         1048 g         1024 t
ORIGIN      
        1 aataaaaaag acgaaatcat ttatataaat gatttcgtct ttctaaatgc aattaaaatc
       61 aggttgccaa attaatcgta gcgatttaac acgattcgac gtttgctatc gctacctgca
      121 acaaccactt ctgtcgtaac acctttgacg ggcgaagaaa cctgatgacg ggtgtcaaca
      181 ttatacgtta actgaccaca atgctgttgt ggttgactat taaccatagc catactgcat
      241 gttgggggac tgatcctgcc attaatcgtt aatgtggcag tctctgaaga gactgcggga
      301 gaagccaatc cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt
      361 tatccctgta gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac
      421 atccactaca cagcacgaag gatgataatg aagcattgcc tgtacgatca ctcgactttg
      481 tagagtttcg gacgaaggtc cgcagaatat tcgcagtatt aaataagtgt tcattaatgc
      541 ggcaaaaaca aatacaccgc atcaatataa catctctgcg gtgtatgaag aataaccaga
      601 atggttaata ggcgttagac cgcgcggaag gaaatctcgc caggaatgac ttcaccctgc
      661 cagtagagct gagctgcaac gcggtcagcc agctggcggt agatagcggt aaattcgctc
      721 tccggacggc tgataaccgt tggcgtgcct ttatcgagat cctcacgtaa ggagatgtgt
      781 aacggcatct gccccagcag ctgtgtatgg tatttttcag ccagtttctc tgcaccaccg
      841 gtaccgaaaa tcggttcatg gtgaccacag ttactgcaaa tatgcacact catgttctcg
      901 acaataccga gcaccggaac ctcgactttc tcgaacatca caatgccttt tttcgcatcg
      961 atcaacgcga tgtcctgcgg ggtggtgacc acgactgcac cagttactgg aatgttttgc
     1021 gccagcgtca attgaatgtc accggtgcct ggcggcatat caagcaccag atagtcgaga
     1081 tccggccaca aggtttcctg caacatctgc attaacgcct tgctcgccat cggtccacgc
     1141 cacaccatcg cattgtcgtc agtgaccaga taaccaattg aattggttgc caggccatga
     1201 gacataatcg gtgccatgtg ggtaccgtcc ggcgaagttg gacgctgatt ttctgcgccc
     1261 agcatggtgg gaattgatgg gccgtagata tcggcgtcca gaatgccgac tttcgcccct
     1321 tccgccgcca gtgccagcgc caggtttacc gccgtggacg atttacccac gccgccttta
     1381 ccggaactca ccgcgataat gttcttcaca ccgttaattc ccggctgatt cttcacgcgt
     1441 ttcagagtgg ctatgttatg cgacagtttc cagtcgatag ctttcgcccc ggtgatgcgc
     1501 agcagttcgg cgctacattg ctcttttaac tcttcgaacg cgctatgcca gacaaacggc
     1561 ataaccagtt ccacatgcaa cgtgtcgtcc atccaggcga catggtgcaa cgctttcagc
     1621 gtggtgaggt tatgcttcag ggttgggtgc tgaaaattgg ccagtgtccc ggcgaccatt
     1681 gcgcgcaagg cttctggtga tttggcctgg gattgttcgt tcatcccgac tccttttttg
     1741 tatagataaa ccatcagctg atagtttacc tgaagaatat agagaagtac ttacttaaca
     1801 ttttcccatt tggtactatc taaccccttt tcactattaa gaagtaatgc ctactatgac
     1861 tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg tacgctaacg gctcaatcca
     1921 cctcggccat atgctggagc acatccaggc tgatgtctgg gtccgttacc agcgaatgcg
     1981 cggccacgag gtcaacttca tctgcgccga cgatgcccac ggtacaccga tcatgctgaa
     2041 agctcagcag ctcggtatca ccccggagca gatgattggc gaaatgagtc aggagcatca
     2101 gactgatttc gcaggcttta acatcagcta tgacaactat cactcgacgc acagcgaaga
     2161 gaaccgccag ttgtcagaac ttatctactc tcgcctgaaa gaaaacggtt ttattaaaaa
     2221 ccgcaccatc tctcagctgt acgatccgga aaaaggcatg ttcctgccgg accgttttgt
     2281 gaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac ggcgataact gcgaagtctg
     2341 cggcgcgacc tacagcccga ctgaattgat cgagccgaaa tcggtggttt ctggtgctac
     2401 accagtgatg cgtgattctg aacacttctt ctttgatctg ccctctttca gcgaaatgct
     2461 gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg gcaaacaaaa tgcaggagtg
     2521 gtttgaatcc ggcctgcaac agtgggatat ctcccgcgac gcgccatact ttggttttga
     2581 aattccgaac gcaccgggca aatatttcta cgtctggctg gacgcaccga ttggctacat
     2641 gggttctttc aagaatctgt gcgacaagcg cggcgacagc gtaagcttcg atgaatactg
     2701 gaagaaagac tccaccgccg agctgtacca cttcatcggt aaagatatcg tttacttcca
     2761 cagcctgttc tggcctgcca tgctggaagg cagcaacttc cgcaagccga ccaacctgtt
     2821 tgttcatggc tatgtgacgg tgaacggcgc gaagatgtcc aagtctcgcg gcacctttat
     2881 taaagccagc acctggctga atcattttga cgctgacagc ctgcgttact actacactgc
     2941 gaaactctct tcgcgcattg atgatatcga tctcaacctg gaagatttcg ttcagcgtgt
     3001 gaatgccgat atcgtgaaca aagtggttaa cctggcctcc cgtaatgcgg gctttatcaa
     3061 caagcgtttt gacggtgtgc tggcaagcga gctggctgac cctgagctgt acaaaacctt
     3121 taccgatgcc gctgaagtga ttggtgaagc gtgggaaagc cgtgaatttg gtaaagcagt
     3181 gcgcgaaatc atggcactgg ctgacctggc taaccgctat gtcgacgagc aggctccgtg
     3241 ggtagtcgca aaacaggaag gtcgcgatgc tgacctgcag gcgatttgct caatgggcat
     3301 caacctgttc cgcgtgctga tgacttacct gaagccggta ctgccgaaac tgaccgagcg
     3361 tgcagaagcc ttcctcaata cggaactgac ctgggatggt atccagcaac cgctgctggg
     3421 ccacaaagtg aatccgttca aggcgctgta taaccgcatc gatatgaaac aggttgaagc
     3481 tctggtggaa gcctctaaag aagaagtgaa agccactacc gcgccagtta ctggcccact
     3541 ggcagatgat ccgattcagg aaaccatcac ctttgacgac ttcgccaaag ttgacctgcg
     3601 cgtggcgctg atcgaaaacg cagagtttgt tgaaggttct gacaaactgc tgcgcctgac
     3661 gctggatctc ggcggtgaaa aacgcaatgt cttctccggt attcgttctg cttacccgga
     3721 tccgcaggca ctgattggtc gtcacaccat tatggtggct aacctggcac cacgtaaaat
     3781 gcgcttcggt atctctgaag gcatggtgat ggctgccggt cctggcggga aagatatttt
     3841 cctgctaagc ccggatgccg gtgctaaacc gggtcatcag gtgaaataat cccccttcaa
     3901 ggcgctgcat cgacagcgcc ttttctttat aaattcctaa agttgttttc ttgcgatttt
     3961 gtctctctct aacccgtata aatactggta gcatctgcat tcaactggat aaaattacag
     4021 ggatgcagaa tgagacactt tatctatcag gacgaaaaat cacataaatt ctggacggtg
     4081 gagcaacagg gaaacgagtt gcatatcagt tggggaaaag ttggcaccaa agggcaaagt
     4141 cagataaaaa gtttttcaga tgctgcggcg gcggaaaaag cggaactgaa gctgattgcg
     4201 gagaaggtga agaag
//
LOCUS       DAECOD010000190         4198 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.190, whole genome shotgun sequence.
ACCESSION   DAECOD010000190 DAECOD010000000
VERSION     DAECOD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.190"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            84..752
                     /locus_tag="KKO15_003893"
     CDS             84..752
                     /locus_tag="KKO15_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362492.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="KKO15_003894"
     CDS             complement(995..1690)
                     /locus_tag="KKO15_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBD6362493.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="KKO15_003895"
     CDS             complement(1683..3110)
                     /locus_tag="KKO15_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBD6362494.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="KKO15_003896"
     CDS             complement(3121..3840)
                     /locus_tag="KKO15_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBD6362495.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAECOD010000191         4168 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.191, whole genome shotgun sequence.
ACCESSION   DAECOD010000191 DAECOD010000000
VERSION     DAECOD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.191"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KKO15_003897"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KKO15_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBD6362496.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KKO15_003898"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KKO15_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KKO15_003899"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KKO15_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="KKO15_003900"
     CDS             3787..>4168
                     /locus_tag="KKO15_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBD6362497.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1207 c         1023 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAECOD010000192         4059 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.192, whole genome shotgun sequence.
ACCESSION   DAECOD010000192 DAECOD010000000
VERSION     DAECOD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.192"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(903..1094)
                     /locus_tag="KKO15_003901"
     CDS             complement(903..1094)
                     /locus_tag="KKO15_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD6362498.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1158..1481
                     /locus_tag="KKO15_003902"
     CDS             1158..1481
                     /locus_tag="KKO15_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD6362499.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1471..1782
                     /locus_tag="KKO15_003903"
     CDS             1471..1782
                     /locus_tag="KKO15_003903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362500.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1757..2179
                     /locus_tag="KKO15_003904"
                     /pseudo
     CDS             1757..2179
                     /locus_tag="KKO15_003904"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2160..2690
                     /locus_tag="KKO15_003905"
     CDS             2160..2690
                     /locus_tag="KKO15_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBD6362501.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2697..2930
                     /locus_tag="KKO15_003906"
     CDS             2697..2930
                     /locus_tag="KKO15_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD6362502.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3476..3943)
                     /locus_tag="KKO15_003907"
     CDS             complement(3476..3943)
                     /locus_tag="KKO15_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362503.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          924 c         1178 g          965 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgccgg gagcagcata
      901 tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg
      961 gcgtggtcgt gaaacgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc
     1021 ttctccagca gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     1081 tcctgtttag tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac
     1141 cgatgaaacg cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     1201 tggaacgctg tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggggagc
     1261 cggttgcccg ttccgggaaa ctgccgacac tggcaccgcc gttactgtgt cagctggctg
     1321 ccatcgggaa taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg
     1381 gtaaccgggt tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc
     1441 gtctggccgt cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa
     1501 ggtggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca
     1561 acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg
     1621 aagaaataca cgtccggtgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt
     1681 gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac
     1741 agcatcctgt gggtggatgc accaggacaa gggacggctt gagctgaatt ttgtcattcc
     1801 gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg cagacaggcc
     1861 tagaattgat gcctggcaga cgctggttaa tcaccattac gggctgcatg acccgaacgc
     1921 cccggagaac cgcaggacgc tgacactccc tgataatctg ccggaaacga aacaggcgat
     1981 tgctgagggc gttacacgcg ggatagacgc gctttaccat gccggagaga taaaaggccg
     2041 tcaggatgtg attcaggctc tcacagaggc agggattgac gtggttaggg tgacgcgaag
     2101 cagtatcagc attgcagacc cgaacggcgg gaagaatatc agactgaaag gagcgtttta
     2161 tgagcaatct tttacagacg ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat
     2221 ctacagagaa aatgctgaac gacgagttca ggaagctcga agaatctgta aacagggaac
     2281 tgacctcaaa cgaatcttta atcaggaacg ctatcaacga ccatacatca gccctgaaag
     2341 aacttctgga gaggtatcag aaaacgaccg tcgatacgat ggacgcacac tggaaaaccg
     2401 tactgaaaat gagcgtgaaa cgctggctgt ggctgattat cgtctcagta ctgatgtttg
     2461 ccacgaccgg gagcctgctg tggtaccagg gcatgaagat aaacgcgaac atgaatattc
     2521 tccgcgagca gaaagagagt ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc
     2581 acgaggacag caacggacgt tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact
     2641 ggacgatgga aaacgggaaa cagaatgccg taaggctggt acaggagtaa cagagcatga
     2701 cggagctgga aagacatttg ctgaacgcat tagaacaact gcaacaggac tatatgcagc
     2761 ggctgaacga atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac
     2821 gggacaacgc gatgctgaac gagcgggtca tgcagttgag tcagcaggtg cagcacttga
     2881 gcgagcagac agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg
     2941 gctgatacag gaatgcgaac atgcgttatc cctggagcgt gagcaccagc tggaaataca
     3001 ggaacggaca ctggatggcc cttcgctggg atggtaatca gagacaggct aaaatgttgt
     3061 cagcgcgttt ccggtgcatt ttttgggata atggttggtt cgggggcacc gggaacgcgc
     3121 ttcagtcaga ccttgctgaa aacataggaa cgcgttaaaa cgcatacggg cgcgattctg
     3181 gtgtgtcttt tgttgctgtg ttttgcctct attgccagat cgcgctcatt cccctgaact
     3241 tcttttttcc cgctcaggtc cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt
     3301 tcagctgtct ctggctggca attcctgcgt gattcacatg gctgcatagc tatgcatgtg
     3361 gttaaaattt accgggtgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc
     3421 tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg cgtacaatta agatattacg
     3481 ttaaattgtg agggaatggt ccccatatac tgactttatc atagggatag ggttggtagt
     3541 gggtcgattg ggtttcggca ttgtttccgt taatgccaat tgcatttttg tatttgatgc
     3601 aacagaaggc gtgagattct cctgggcctg gaaaggataa gtgtttagaa ttgatggcaa
     3661 aatatcttcc atccgatttt tgaagtagtt gttgtttggc tgtctcccaa agggtgttgt
     3721 tacaaacttc ttgaatttgt agtttttgca ggactcttat tactgcgtta tcatgttcca
     3781 attctttagg ggagtgtatt attctctctt ttaataagct gtttaccaca gggtttagtt
     3841 gttgtctaaa gcacgcagat aatgccattg ctacacaatt ttgttcccga ggaagcatgt
     3901 ttggcggggt atgccgtgtt tgatgtgata tgtctattct catagaaggt ctctggctta
     3961 tagcatcaac aaatacggct ataaaacaaa attttcatag agtgattcaa gatcttcttg
     4021 agatcctttt ttgcggcgcg taatctgctg cctgcaaac
//
LOCUS       DAECOD010000193         3657 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.194, whole genome shotgun sequence.
ACCESSION   DAECOD010000193 DAECOD010000000
VERSION     DAECOD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.194"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="KKO15_003908"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="KKO15_003908"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBD6362504.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="KKO15_003909"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="KKO15_003909"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBD6362505.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="KKO15_003910"
     tRNA            2656..2731
                     /locus_tag="KKO15_003910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3657)
                     /locus_tag="KKO15_003911"
     CDS             complement(2746..>3657)
                     /locus_tag="KKO15_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6362506.1"
                     /translation="FWLQHFAGMQLRDITESKIYSAMQKMTNRRHEENWKLRAESCRK
                     KGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWL
                     EPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSN
                     RAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAA
                     LRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEH
                     ARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          847 a          917 c          982 g          911 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc attaaaatca ataagttaca catcattagt
     2761 accttcctta ttttttgact gggacaaatt tgggaccgat gggttcagga tcgagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgacaac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgattccc
     3121 gatttgtttt ttcaatacgc gacatgcagt atcattcagc gcaacgccaa ttgcgcggtt
     3181 tgatttactc tcttccgggt ttatccatgc cacccggcgc tgcatatcta tttgttgcca
     3241 ttcaaggttg atgatgttcg agcgtcttaa gcctgttgcc agtgcaaatt caacaacaga
     3301 ctttaatggc tccggacatt catcaatcag cctttgtgct tcatggggct ccagccagcg
     3361 gatccgttta ttctttggtt gaggcacttt aataattggt gccttatcca gcattttcca
     3421 ttcacgctct gcggctctta gtagggcctt tataaatgaa agatgcgtag ccttcgttgc
     3481 aacggacgct ggttttggcg tgtattctgg aacaggtttc cctttttttc tgcatgattc
     3541 tgccctgagt ttccagtttt cctcatgacg ccggttcgtc attttctgca ttgctgaata
     3601 aatttttgat tcagtgatgt ctcttagttg catccctgcg aaatgttgaa gccagaa
//
LOCUS       DAECOD010000194         3610 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.195, whole genome shotgun sequence.
ACCESSION   DAECOD010000194 DAECOD010000000
VERSION     DAECOD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.195"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            31..510
                     /locus_tag="KKO15_003912"
     CDS             31..510
                     /locus_tag="KKO15_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362507.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            498..731
                     /locus_tag="KKO15_003913"
     CDS             498..731
                     /locus_tag="KKO15_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBD6362508.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            731..2197
                     /locus_tag="KKO15_003914"
     CDS             731..2197
                     /locus_tag="KKO15_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD6362509.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2088..2831
                     /locus_tag="KKO15_003915"
     CDS             2088..2831
                     /locus_tag="KKO15_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD6362510.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2835..>3610
                     /locus_tag="KKO15_003916"
     CDS             2835..>3610
                     /locus_tag="KKO15_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD6362511.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSD"
BASE COUNT          923 a          890 c         1030 g          767 t
ORIGIN      
        1 atcatcaaac tgtcttggat agaagccgca gtggatgcgc acaaaacgtt aaatttcgaa
       61 cccagtggaa gaaagcgtat tggctttgac gtggctgaca gtggtacaga taagtgcgct
      121 aacgtttacc gtcacggatc cgttgttttc tgggccgacg aatggaaggc caaagaagat
      181 gaattactga agagctgcca gcgtacttat caggcggcgc tggagcgtga agcagatatt
      241 gtttacgact ctatcggtgt tggtgcgtct gccggtgcta aattctctga aattaacgct
      301 gaccggaaga gcgagaacgc atacgcgcga cgtgtgaatt accagaggtt taacgccggt
      361 gctggtgtgc atgagccaga tgacgagtac aacggcatcc ccaacaaaga ctttttcgca
      421 aatcttaagg ctcaggcatg gtggctgacc gtttcagaaa tacgtttaac gccattaaca
      481 acggagaaca gtatcctgtg gatgagctga tcagcataga ttctcgttgt ccgttgcttg
      541 aaaagctgaa actggaactg acaacacctc atcgtgattt cgaccgtaac ggacgtgtga
      601 tggtcgaaag taaaaaagac ctcgcaaaac gcgagatacc gtcaccaaac gttgctgatg
      661 cattcattat ggccttcgcg ccaatcgata catcgctgga tatctgggaa cagctgggga
      721 gacaggcctg atggcacgaa acaaacaagc cctgcggcga actgtgcagg ccacagctga
      781 tggttatgag aattttattg cccgagtagg gatgcagaca cctaaccagc actcagcatc
      841 cacctaccgg gctaatttca ccagtcgtaa ccgcatgctg gtggaatggt cctatcgttc
      901 atcctggatc atcggcgaag cagtcgatgc tatcccggat gatatgaccc gcaaaggcat
      961 tcgcatcact tcggaaattg atgcaaaaga tcgtggcatt ctcgaatcac aactggatga
     1021 gttgcaaatc tgggatgcgc tgaatgacgt gctgaaatgg tcgcgcctct acggcggcgc
     1081 ggtgggtttc atcatgattg aggggcaggc accaatgacc ccgctgcgac ccgaaaccat
     1141 cggtaagggc aagtttaagg ggattctccc gctcgaccgc tggatggtcg acccggcact
     1201 gacccgccgc attaaagata tggggccgga cctgggtaaa cctgagtttt acgatgtggt
     1261 gaccacagca acgggaattc ctgcctggcg cattcatcac agtcgcctga ttcgctttga
     1321 tggcgtcacg ttgccatttc agcagaagat gaccgagaac gaatggggaa tgtcggttgt
     1381 agagcgtatc tgggatcgtc ttaccgcgtt cgacagcgct actgtcggcg cggcgcagct
     1441 ggtctacaag gcgcatttgc gtacctacag cgtggagaag ctacgcgagc ttatcgcact
     1501 tggtggtcct gcgtatgaag cgttgctgaa gaatatcgac ctgattcgac agttccagag
     1561 taatgaaggc atgacgctca tggactcgcg ggataagttt gaaacccatc agtacagctt
     1621 cagtggtctg gatgacatcc tttcgcagtt tgcagaacag attagtggcg ctgttggtat
     1681 cccactggtg cggttgttcg gacagtcccc gaaaggattt tctaccggtg atgcagacct
     1741 tgccaactat tacgatcgca tcagttcgtt gcaggagagg cgtttacgtc ttccggtgcg
     1801 gcggatactg gacatcatgc atcgttcgga acttggcaag ccgctgccgg acgatttcac
     1861 gtttgagttt aacccgctct ggcaaatgtc tgatgtcgat cgctcaacgg tggcgttaaa
     1921 caccaccaac gcaatcagta cggcgctggg tgatggtctg atgacactga aagccgctat
     1981 gactgatttg cgagaaaatt ctgacgtaac cggcataggg gcatccatta ccaacgagga
     2041 catcgagaat gccgaagatg aagcgccgcc cggcatcggc gaacctgatg acgaaccgca
     2101 ggagccgtca ggcggaaatc cggtatcgaa ccagcctacg caggatagcg agggcggtcg
     2161 gagacatcgt aaatggtcgc tacgatggtt caaatgacag tatcacggaa attattgcgg
     2221 cgctggagcg ttacagtgaa atcatcaccc cctgggcgac aaaggtcgcg gaaaacttta
     2281 ctgcggacct aacccggcag aacgagaaag tttggcggca acacagcaag aacatcagtc
     2341 gcgagctccg caatcttgtg gaaagcgctc ctgtgggcca ggtgatgcaa tccatcatcg
     2401 ccgaacaggt caagtacatc aaatctctgc ctctcgaggc cgcagacagg gtgtacgaca
     2461 tccagaacaa agcgatagag gctgttgtca ccggtggtcg ggcggagcag tttgctaaag
     2521 agattgcatc taccggcgat gttgctaagt ctagggccga tctgattgcc cgaacggaac
     2581 tgggaagagc aacgggcgcg ctggatatgg cccgagcgat agctattggc tcggatggtt
     2641 atatttggcg tacagccgat gatggcgatg tccgagattc ccacgatcac atgaaaggta
     2701 aattcgtccg ctgggattca cctccaactt tggacggcat gaccggccac gcgggcgagc
     2761 tgccaaactg ccgctgctat aaagagatcg tgtttgttcg cgttccattc gcaatgaaaa
     2821 gggcagcata acccatgaaa tacttttttg agaccaggct cggggaaacc cgataccgcc
     2881 tggctgacgg ctcgttgctg tgcaaagacg tgccgatagg acgaacaggt aagcagctct
     2941 atggtgctga tgacctgcca aaactgaaac ccgataagtt cgttgaaata gtcgtcacgc
     3001 gttctcctga gcaggtattc catccggcca cgcttgcctc attcgaaggg atgagcatca
     3061 cgatcctgca tcctgaagat gaaaacggga atgtgcggct ggtaaatccc gagaactgga
     3121 aagtgcttgc tgtcgggcac ctccagaatg tccggcgcgg gacgggtgag cagtctgatt
     3181 tgatgctggc tgaccttatc gtcaaagacg aaagcgccat tcagcttatc gaagatggcc
     3241 tgcgcgaagt gtcgtgcggc tatgacgcgg agtacgagca gaccgagcca ggtaaagccg
     3301 agcaggtcga tattaccgga aaccatgtgg ctcttgtccc taaaggcaga gccggaaatc
     3361 gttgtgcaat tggagacaga gacacaatgg caaatcaaaa gaaaaactgg tggaaccgca
     3421 tgcgtgcggc catcaagaca ggagatgccg acaccatgaa cgaactggtg gagtcggctc
     3481 ccgcatcggt tacaggagat gagggggatt tgccgcaggg cgttaatctc aacatcaacc
     3541 tgtccccgca gcaaccacta ccggacaaag caccagagat gggcggagat ccaaccggcg
     3601 acagtgatga
//
LOCUS       DAECOD010000195         3590 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.196, whole genome shotgun sequence.
ACCESSION   DAECOD010000195 DAECOD010000000
VERSION     DAECOD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.196"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            32..493
                     /locus_tag="KKO15_003917"
     CDS             32..493
                     /locus_tag="KKO15_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBD6362512.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KKO15_003918"
     CDS             complement(534..1004)
                     /locus_tag="KKO15_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBD6362513.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KKO15_003919"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KKO15_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBD6362514.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KKO15_003920"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KKO15_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBD6362515.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          952 a          926 c          818 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAECOD010000196         3548 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.197, whole genome shotgun sequence.
ACCESSION   DAECOD010000196 DAECOD010000000
VERSION     DAECOD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.197"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KKO15_003921"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KKO15_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD6362516.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KKO15_003922"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KKO15_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBD6362517.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KKO15_003923"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KKO15_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBD6362518.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAECOD010000197         3495 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.198, whole genome shotgun sequence.
ACCESSION   DAECOD010000197 DAECOD010000000
VERSION     DAECOD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.198"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            337..1395
                     /gene="rfaQ"
                     /locus_tag="KKO15_003924"
     CDS             337..1395
                     /gene="rfaQ"
                     /locus_tag="KKO15_003924"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBD6362519.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1392..2516
                     /locus_tag="KKO15_003925"
     CDS             1392..2516
                     /locus_tag="KKO15_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBD6362520.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2509..3306
                     /gene="rfaP"
                     /locus_tag="KKO15_003926"
     CDS             2509..3306
                     /gene="rfaP"
                     /locus_tag="KKO15_003926"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBD6362521.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          925 a          754 c          844 g          972 t
ORIGIN      
        1 tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt
       61 ttatgcgatt cattgatttc tgctgtacgt gcaggaagga atcgcagagc aacagtgaaa
      121 gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca acaatgtgct
      181 taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg tcaatatcag
      241 tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt tttctgcttc
      301 accttaatcg gataatctca acaaaaagag ttacttgtgg ataagccatt tcgaagaatt
      361 ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt cattagttct
      421 ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga caccatcccg
      481 atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa aaaagcaaaa
      541 gcctcagaaa aaattgccaa cttttttcat ctcatcaagg tattacgtgc caataagtat
      601 gaccttatcg tcaatcttac cgatcaatgg atggttgcta tactggttcg cttattaaat
      661 gctcgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg gcgtaaaagt
      721 ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt atccgtgctg
      781 acaccattgg gacttgattc gttggtgaag cagacaacca tgagttaccc gcctgcaagc
      841 tggaaacgta tgcgtcgcga acttgatcac gctggtgttg gacaaaatta tgtggttatc
      901 caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt
      961 gatgccttac atgctcgtgg ttatgaagtc gttctgacgt ccggcccaga taaagacgat
     1021 ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac ggcgctggct
     1081 ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct gtttattggc
     1141 gttgattccg caccggcgca tattgccgct gcagttaata cgccgctgat atcgctgttt
     1201 ggcgcaacag atcatatttt ctggcgtccc tggtcaaata acatgattca attctgggcg
     1261 ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg
     1321 gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc ctccacgaca
     1381 ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt ggcggtttgc
     1441 agcgcgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat gttcgggttt
     1501 atacccagtc gtgggaaggc gaatgccctg atgtatttga agtgatcaaa gtgccggtta
     1561 aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac
     1621 gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg gacgtttatt
     1681 atgccgctga tgtttgttat gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc
     1741 tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa cagggcaagc
     1801 cgacacagct gctgatgctg acagataagc aaatcgccga tttccagaaa cattatcaga
     1861 ctgaagcgga gcgttttcat attctgccac cggggattta tcctgatcgt aaatatagcc
     1921 agcagccagc caatagccgt gaaatcttcc gtaagaagaa tggaataacc gaacaacaat
     1981 atttattgtt gcaggtcggt tcagacttca cgcgtaaagg tgccgatcgt tccattgaag
     2041 cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt gttgggcagg
     2101 ataaaccgcg aaaatttgag gcactggcag aaaaacgcgg cgtgcgcagt aatgttcact
     2161 tcttctcggg gcgcaacgat gtctcggaat taatggcggc ggcggattta ttactgcatc
     2221 ctgcctacca ggaagcggcg ggaattgtgc tgctggaagc gattactgca ggattaccgg
     2281 tactaacaac tgccgtttgt ggctatgcgc attatattgt cgacgctaat tgcggcgagg
     2341 ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa gcgttaacgc
     2401 aatcttcatt gcgccaggct tgggcggaaa atgcgcgaca ttatgctgat acacaagatt
     2461 tatacagtct gccagagaaa gcggcggata tcataacggg tggtctggat ggttgaactt
     2521 aaagagccgt ttgccacgtt atggcgcggc aaagatcctt ttgaggaagt taaaaccttg
     2581 cagggtgagg tatttcgtga actggaaact cgccgtactc tgcgctttga aatggcggga
     2641 aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat caaaaattta
     2701 ctctcattgc ggatgccagt attaggcgca gaccgcgaat ggaatgcgat tcatcgactg
     2761 cgggatgtcg gcgttgatac tatgtatggg gtggcattcg gcgaaaaagg catgaatccg
     2821 ctgaccagaa cctcgtttat tattaccgaa gatctgacac caaccatcag tctggaagat
     2881 tactgtgctg actgggcgac caacccacca gatgttcgcg taaagcgtat gcttattaag
     2941 cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccatcg tgactgttat
     3001 atctgtcatt tcctgctgca cttgcctttt tccggtaagg aagaggagtt aaaaatttcg
     3061 gtaattgacc tacaccgggc gcagcttcgc acgcgcgttc caggtcgttg gcgcgataaa
     3121 gatcttatag ggctttattt ttcttcaatg aatattggcc tgactcagcg ggatatctgg
     3181 cgctttatga aggtgtattt tgccgccccg cttaaagaca ttctcaagca ggaacaagga
     3241 ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat tcgaaaatcg
     3301 ttgtaacccg taacttattt ttgccaaaat tttggataca gaataaatat gtctcaactc
     3361 aatgatagtg acatcatcct ttttgagtat aattttcatt atcaaaatat aagatctaaa
     3421 aatactcttg atatagcatt tggtattgac agaaattttc tttttggatg tggtgtagcc
     3481 atcgcatcta ttcta
//
LOCUS       DAECOD010000198         3326 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.199, whole genome shotgun sequence.
ACCESSION   DAECOD010000198 DAECOD010000000
VERSION     DAECOD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.199"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            455..1282
                     /locus_tag="KKO15_003927"
     CDS             455..1282
                     /locus_tag="KKO15_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD6362522.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1326..2075
                     /locus_tag="KKO15_003928"
     CDS             1326..2075
                     /locus_tag="KKO15_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD6362523.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2072..2641
                     /locus_tag="KKO15_003929"
     CDS             2072..2641
                     /locus_tag="KKO15_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362524.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2765..3007
                     /locus_tag="KKO15_003930"
     CDS             2765..3007
                     /locus_tag="KKO15_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD6362525.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLVEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3011..3157
                     /locus_tag="KKO15_003931"
     CDS             3011..3157
                     /locus_tag="KKO15_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362526.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEDWCHCRG
                     LSVV"
     gene            3166..>3326
                     /gene="xisR"
                     /locus_tag="KKO15_003932"
     CDS             3166..>3326
                     /gene="xisR"
                     /locus_tag="KKO15_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBD6362527.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          930 a          797 c          855 g          744 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca
      421 tttataaggg tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc
      481 ccttgttctg ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg
      541 catcaaggta aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg
      601 ctactggacg ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac
      661 ctacgatgag ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg
      721 cgtggcctac aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg
      781 cgtggacgac attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact
      841 ggccagcaaa atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc
      901 agctcttaaa gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc
      961 atatgtgggt aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac
     1021 ggcgaaccag aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa
     1081 catgttgcgc gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga
     1141 agccgatgct attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct
     1201 ggaagccatc aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac
     1261 accatttatt caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga
     1321 taaaaatgaa tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc
     1381 agcgtgttgt gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc
     1441 gtgcagcatt caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag
     1501 gtgcggacgt aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg
     1561 ggattgtact ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg
     1621 cctggcaggt tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg
     1681 aaatcgagat gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc
     1741 atgtggaaga acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata
     1801 tgcgcgccgg atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca
     1861 agtgtcggaa tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc
     1921 cggacggtct gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc
     1981 accaaatgat gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc
     2041 ttttccaggt gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc
     2101 cgctctggtg tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac
     2161 gccgaaacat attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg
     2221 gacgaagaaa aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac
     2281 agagctgatt tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact
     2341 gtccattacc ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc
     2401 gaaaaagttt gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga
     2461 gaagatgttt ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg
     2521 gcaagcggtc agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt
     2581 tgttgtgatg ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg
     2641 aaaatcgaat accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta
     2701 actctgggga tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa
     2761 ccacatgttc gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt
     2821 agaaattact cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg
     2881 gcgagtacgc atcggtgaat tcagtagcct ggtcgagcat attgaccacc tggaatatca
     2941 caagattctg gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag
     3001 gaagtaaaaa atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta
     3061 cgacctgacg cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga
     3121 ggactggtgc cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt
     3181 caactggctc ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa
     3241 ccaggcacaa ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac
     3301 gtatcaccag acggtaatcc aaagcc
//
LOCUS       DAECOD010000199         3207 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.200, whole genome shotgun sequence.
ACCESSION   DAECOD010000199 DAECOD010000000
VERSION     DAECOD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.200"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            130..1230
                     /gene="ompC"
                     /locus_tag="KKO15_003933"
     CDS             130..1230
                     /gene="ompC"
                     /locus_tag="KKO15_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD6362528.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1394..1879
                     /locus_tag="KKO15_003934"
     CDS             1394..1879
                     /locus_tag="KKO15_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD6362529.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1989..2978
                     /locus_tag="KKO15_003935"
     CDS             1989..2978
                     /locus_tag="KKO15_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD6362530.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1069 a          635 c          552 g          951 t
ORIGIN      
        1 atattattct gtttttctca ttgtttttaa tcgccattct attttaaaaa cagtatgtca
       61 tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata attaaaagga
      121 ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac
      181 gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct ttatggcaag
      241 gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa aacttatgcc
      301 cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt tggtcactgg
      361 gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa caagacccgt
      421 ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg tcgtaactac
      481 ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg tggcgatacc
      541 tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac ctatcgcaac
      601 tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca aggcaaaaat
      661 gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa cggtgacggt
      721 ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt atataccaaa
      781 tccgatcgga caaatgcgca ggaaagagcc gcagctaatc ctctcaatgc ctccggtaag
      841 aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta ctttgcagct
      901 aattacgatg aaacattaaa catgaccacc tatggggatg gttatatctc taacaaagca
      961 caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc atcactcgct
     1021 tacctgaaat cgaaaggcag agatctgggc cgctacgccg accaggacat gattgagtat
     1081 atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt tgattataaa
     1141 atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcgcac cgataacatc
     1201 gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc ttctctgggc
     1261 tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca attcttgata
     1321 tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat cttactataa
     1381 tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa ataatgccag
     1441 ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt tttgtgaacg
     1501 actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa ttgccgtgga
     1561 gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat atcttgattt
     1621 gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa acaattgtct
     1681 aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca gcgttaataa
     1741 aattaacacg ataacctttc caaaagaacc aaaactggaa aacatttata ttgatcataa
     1801 caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg aagcgcaaaa
     1861 taatagccta aaaaaatgat atttctgata gttataaact gaaatttctt aatcttgatt
     1921 ataacaagct agcatcgctg gatgtctccc ggcaagaatc tctgattgag ttaagtgccc
     1981 atcacaatat gatcactgac cttatattac acaatcatcc caggatgaaa acaatcactt
     2041 taaacgacaa ccatattgca catttaaacg ccaaaaacac tacaaaactg gaatatttaa
     2101 acttaagcaa taacaattta ctgccaacca atgacattga tcaactaata tcatcaaaac
     2161 atctttggca tgtattagtt aacggcatca acaatgatcc acttgcacaa atgcagtact
     2221 ggactgcagt aagaaatata attgatgaca ctaatgaagt gaccattgat ttatcaggac
     2281 ttaatttaac cactcaacca ccagggctgc aaaacttcac ctctatcaat cttgataata
     2341 accaactcac acattttgat gcaaccaact acgatagact cgtaaagcta agtctgaata
     2401 gtaatgctct tgagtcaata aattttcctc aaggcagaaa tgtaagtatt acacatatat
     2461 ctatgaataa taatgctctc agaaatattg atatagatag gctttcatca gttacttatt
     2521 ttagtgcggc acataatcaa ctagagtttg tgcaattaga atcttgcgaa tggctgcaat
     2581 acctgaatct cagccataat caattaactg atattgttgc aggtaataaa aatgaactct
     2641 tactgctgga tctatcccat aataaactaa caagtttaca caatgtctta tttcccaact
     2701 tgaatacgtt acttattaac aacaatttgc tttctgaaat taaaatattc tatagcaact
     2761 tccgcaatgt tcagacatta aacgctgcta acaaccagtt gaaatatata aatcttgatt
     2821 tcctgactta tcttccatct atcaaaagtt taagactgga caataataaa ataacccaca
     2881 ttgatactaa taatacatcc gatattggaa ctttattccc cataataaaa cagagcaaaa
     2941 acttaaattt tttaaatgtt tctgggaaga acaattgacc tactatgcag ctcatgttat
     3001 ttaatttgtt ttccccagca cttaagctta atactggcct ggcaattctt tcgcctggtg
     3061 catttgaagt tcactctgac ggaatagatg cggataacga attgtttcac tatactatta
     3121 aaaaagcata taccccatat aatatacaca cttacaagac agaggaagtt gtaaaccaga
     3181 ggaatataaa agttaaaaac atgacct
//
LOCUS       DAECOD010000200         3180 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.201, whole genome shotgun sequence.
ACCESSION   DAECOD010000200 DAECOD010000000
VERSION     DAECOD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.201"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="KKO15_003936"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="KKO15_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBD6362531.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANADGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="KKO15_003937"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="KKO15_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          803 a          659 c          774 g          944 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggatgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt agtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAECOD010000201         3068 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.202, whole genome shotgun sequence.
ACCESSION   DAECOD010000201 DAECOD010000000
VERSION     DAECOD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.202"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..497
                     /locus_tag="KKO15_003938"
     CDS             <1..497
                     /locus_tag="KKO15_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6362532.1"
                     /translation="GEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIP
                     LVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDD
                     AESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVT
                     LDYR"
     gene            577..1272
                     /locus_tag="KKO15_003939"
     CDS             577..1272
                     /locus_tag="KKO15_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD6362533.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1301..>3068
                     /locus_tag="KKO15_003940"
     CDS             1301..>3068
                     /locus_tag="KKO15_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6362534.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          887 a          718 c          684 g          779 t
ORIGIN      
        1 atggcgagat tttaaaatcc gcttgtgaaa tcaatgactc tgataagaaa attgaagttg
       61 ctcttggtca ctataatgcc gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc
      121 cattcactat tcctttagtt aactgcccag tgactgggtg ggagcacgat aacggcaacg
      181 ttgaagcgtc tttccgttta tggctggaaa cgcgggataa tggcaccgtc cctaacttcc
      241 ctaatctggc aaaagtgggc tcttttgctg gcacagcgac aacaggtgtg ggtatccgta
      301 ttgacgatgc ggaaagcgga aaccttatgc cactgaatgc tatggggaat gataacacga
      361 tttatcagat cccagcggac tccaatggta ttgtcaatgt tgacctcatc gcttactacg
      421 tatcaaccgt tgaagcatca gaaatcaccc caggggaagc agacgctgtc gttaacgtaa
      481 cgctggatta ccgttaatac gttacggcgt tatctgacct ttcagataac gcccttttcc
      541 ttcctctttc tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg
      601 atattaacct tgttgtgtag cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta
      661 cagcgaacgc gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac
      721 aaaggcgcag aaacgcctta tttactgcaa tcatgggtag ataatataga tggtaaaagc
      781 cgtgccccat ttattataac cccaccgcta tttcgtcttg aggctggcga tgactcatca
      841 ctgcgaatta ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt
      901 aatgttcgtg ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg
      961 ctggtattta aaacgcgggt caaaatgttt tatcgccccg cacacctgaa gggacgggta
     1021 aacgatgcgt ggaaatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac
     1081 ccaactgaat attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc
     1141 aaaattgaat atatcgcgcc cggagaacat aaacagttac cacttccagc atctggcgga
     1201 aagaacgtga aatgggctgc gatcaacgat tatggcggca gttccgggac agaaactcgt
     1261 ccactgcaat aaaaaatata aaaacacagg ccatcaggga atgccacaac gacaccacca
     1321 gggacataaa cgcacaccga aacagttggc gctcattatc aaacgttgtt tgccgatggt
     1381 gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc
     1441 cattatgctg gaaactacaa aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa
     1501 aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt
     1561 ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac
     1621 ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa
     1681 agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt
     1741 tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc
     1801 aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta
     1861 ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct
     1921 gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac
     1981 acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa
     2041 ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag
     2101 ctacgccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg
     2161 cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca
     2221 aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct
     2281 ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacaca
     2341 acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa
     2401 atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga
     2461 atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct
     2521 gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact
     2581 gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt
     2641 tcatggctat caatggcgta ctcagtacat caaagatatc ccggaaacca acaccaatat
     2701 cgctgttagc tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac
     2761 ccgcaattgg ggctataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac
     2821 aatatttgat ggggtaagtc tgtatgcctc cggttcacag caagactatt ggggcaataa
     2881 cgagaaaaac agtaatatct ctgttggggt ttccggccag caatggggaa ttggttacag
     2941 cctgaattat caatacagcc gctacactga tgaaaataat gaccgtgcac tctctttgaa
     3001 tctcagtatt ccgttagaac gctggtggat ttgcccctat atttccagac atctgttatc
     3061 acttaacc
//
LOCUS       DAECOD010000202         3047 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.203, whole genome shotgun sequence.
ACCESSION   DAECOD010000202 DAECOD010000000
VERSION     DAECOD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.203"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(15..473)
                     /locus_tag="KKO15_003941"
     CDS             complement(15..473)
                     /locus_tag="KKO15_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBD6362535.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            593..1402
                     /gene="proC"
                     /locus_tag="KKO15_003942"
     CDS             593..1402
                     /gene="proC"
                     /locus_tag="KKO15_003942"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBD6362536.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1419..2534)
                     /gene="adrA"
                     /locus_tag="KKO15_003943"
                     /gene_synonym="dgcC"
     CDS             complement(1419..2534)
                     /gene="adrA"
                     /locus_tag="KKO15_003943"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBD6362537.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            2685..3047
                     /locus_tag="KKO15_003944"
                     /pseudo
     CDS             2685..3047
                     /locus_tag="KKO15_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    2966..>3047
                     /locus_tag="KKO15_003944"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          743 a          845 c          791 g          668 t
ORIGIN      
        1 ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt ctccagctcc
       61 gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt ctggatccca
      121 ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat ggtcgctggc
      181 gtgtaacgtt cgccgcgcga attaagcgcc gctgcgcctt tctcgatggc ttcagcagcc
      241 aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac aatttcgtta
      301 tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga tggcggcacg
      361 cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc cgcgcgatac
      421 aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt cataaagtca
      481 tcctttgtta ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata gttctgttta
      541 tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg caatggaaaa
      601 gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg gtctgattgc
      661 cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccgg ataaagtcgc
      721 cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag tggcgcaaat
      781 cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc ttagcgaaat
      841 cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg tcacgctcga
      901 ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc cgaacactcc
      961 cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa ccccagaaga
     1021 taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa ttgctgagcc
     1081 gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtat ttatgtttat
     1141 cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg cgtataaatt
     1201 tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag aacatccggg
     1261 ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg tacgcgtact
     1321 ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta tggaaaaatc
     1381 agaaaaactc agcaaatcct gatgactttc gccggacgtc aggccgccac ttcggtgcgg
     1441 ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa ccactcacga
     1501 tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa agttacctgt
     1561 ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat ggcggtaatg
     1621 gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc aaaccgacca
     1681 atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca
     1741 tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc
     1801 agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt
     1861 tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gagtactcat gacctgcaac
     1921 ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacatcaga
     1981 ggataaacga caataatggg aagggagagc caccattcca gcggagcact attgaacgac
     2041 acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc
     2101 gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacagaat catcaacata
     2161 gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca
     2221 tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc
     2281 caggctaaat gcggccagac gaacgcccag ccgaccaaca ccagccacca ccagcccggc
     2341 ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgca
     2401 cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc gttttgagga
     2461 gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt
     2521 atttttggga acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa
     2581 gtatgaaatt tcgggtgagc cactttcgta gctcacccgt tagcactatc tgaaccgccc
     2641 cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact aaaaatactc
     2701 gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt cagggcgaat
     2761 atgactcaca atgggcgaca atttgttcca ttgccccaaa gattggctgt acgccggaga
     2821 ctctgcgtgt ctggggtcgc cagcatgagc gggataccgg gggcggtgat ggagggctca
     2881 ccaccgctga acgtcagcgt ctgaaagagc cggagcgtga aaatcgtgaa ctgcgccgca
     2941 gtaacgatat ccttcgccag gcttccgctt attttgcgaa ggcggagttc gaccgcctct
     3001 ggaaaaaatg atgccactgc tggataagct gcgtgagcag tacgggg
//
LOCUS       DAECOD010000203         3041 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.204, whole genome shotgun sequence.
ACCESSION   DAECOD010000203 DAECOD010000000
VERSION     DAECOD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.204"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            88..153
                     /locus_tag="KKO15_003945"
                     /pseudo
     CDS             88..153
                     /locus_tag="KKO15_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="KKO15_003946"
     CDS             381..629
                     /locus_tag="KKO15_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD6362538.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="KKO15_003947"
     CDS             complement(745..1014)
                     /locus_tag="KKO15_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD6362539.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="KKO15_003948"
     CDS             1071..1739
                     /locus_tag="KKO15_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6362540.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>3041
                     /locus_tag="KKO15_003949"
     CDS             2055..>3041
                     /locus_tag="KKO15_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6362541.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT          913 a          687 c          603 g          838 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgttacaaca aaccgcccaa ccggactacc
     2761 acggcccacg gatttacttc tccatgagtg acggacaaca gaatacactc catcgccccc
     2821 tggctgatgc cgtgacagca tggttcccgg aaaacaaaca atctgatgta tcacagatat
     2881 ggcatgcttt tgaacatgaa gagcacgcca acaccttttc cgcgttcctt gaccgccttt
     2941 ccgataccgt ctctgcacgc aatacctccg gattccgtga acaggtcgct gcatggctgg
     3001 aaaaactcag tgcctctgcg gagcttcgac agcagtcttt c
//
LOCUS       DAECOD010000204         2958 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.206, whole genome shotgun sequence.
ACCESSION   DAECOD010000204 DAECOD010000000
VERSION     DAECOD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.206"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..2850
                     /locus_tag="KKO15_003950"
     rRNA            <1..2850
                     /locus_tag="KKO15_003950"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          785 a          646 c          921 g          606 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgta
//
LOCUS       DAECOD010000205         2951 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.207, whole genome shotgun sequence.
ACCESSION   DAECOD010000205 DAECOD010000000
VERSION     DAECOD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.207"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="KKO15_003951"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="KKO15_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBD6362542.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="KKO15_003952"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="KKO15_003952"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBD6362543.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="KKO15_003953"
     CDS             2603..>2951
                     /locus_tag="KKO15_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD6362544.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAECOD010000206         2890 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.208, whole genome shotgun sequence.
ACCESSION   DAECOD010000206 DAECOD010000000
VERSION     DAECOD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.208"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="KKO15_003954"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="KKO15_003954"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBD6362545.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="KKO15_003955"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="KKO15_003955"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBD6362546.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="KKO15_003956"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="KKO15_003956"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBD6362547.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="KKO15_003957"
     CDS             complement(1896..2810)
                     /locus_tag="KKO15_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBD6362548.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          759 a          668 c          772 g          691 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca gggtccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct
//
LOCUS       DAECOD010000207         2880 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.209, whole genome shotgun sequence.
ACCESSION   DAECOD010000207 DAECOD010000000
VERSION     DAECOD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.209"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..241
                     /locus_tag="KKO15_003958"
     CDS             <1..241
                     /locus_tag="KKO15_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362549.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="KKO15_003959"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="KKO15_003959"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBD6362550.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="KKO15_003960"
     CDS             2140..2856
                     /locus_tag="KKO15_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6362551.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          798 a          635 c          639 g          808 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
//
LOCUS       DAECOD010000208         2808 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.210, whole genome shotgun sequence.
ACCESSION   DAECOD010000208 DAECOD010000000
VERSION     DAECOD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.210"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            6..81
                     /locus_tag="KKO15_003961"
     tRNA            6..81
                     /locus_tag="KKO15_003961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KKO15_003962"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KKO15_003962"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KKO15_003963"
     CDS             complement(299..1057)
                     /locus_tag="KKO15_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBD6362552.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KKO15_003964"
     CDS             complement(1065..2168)
                     /locus_tag="KKO15_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBD6362553.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2808)
                     /locus_tag="KKO15_003965"
     CDS             complement(2178..>2808)
                     /locus_tag="KKO15_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBD6362554.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMIMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tgatcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaac
//
LOCUS       DAECOD010000209         2756 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.211, whole genome shotgun sequence.
ACCESSION   DAECOD010000209 DAECOD010000000
VERSION     DAECOD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.211"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..476
                     /locus_tag="KKO15_003966"
     CDS             <1..476
                     /locus_tag="KKO15_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6362555.1"
                     /translation="CPLLPVHLKESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYK
                     ESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLS
                     VLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            825..1997
                     /locus_tag="KKO15_003967"
     CDS             825..1997
                     /locus_tag="KKO15_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD6362556.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELMPTLTNNNQ"
     gene            complement(2051..2674)
                     /locus_tag="KKO15_003968"
     CDS             complement(2051..2674)
                     /locus_tag="KKO15_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6362557.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          849 a          572 c          530 g          805 t
ORIGIN      
        1 agtgtccgct attgccagta cacctcaaag agagtaaatc aaaccgcgca attggcgttg
       61 cgctgaatga tactgcatgt cgcgtattga aaaaacaaat cggaaatcat caccgttggg
      121 tatttgtgta caaggaaagc tgtaccaaac cagacggaac gaaagcgcca acagtaagga
      181 agatgcggta tgacgcaaac acagcctgga aagcggcgct gagacgggct ggtattgatg
      241 atttcagatt tcacgacttg agacacacct gggcaagttg gctggttcaa gccggagtcc
      301 cgttatcagt gttacaggaa atgggaggct gggagtctat cgaaatggtt cgtcgatatg
      361 ctcacctcgc acctaatcac cttaccgaac acgcacggca aatagacttg attctgaacc
      421 catcggtccc aaatttgtcc cagtcaagaa ataaggaagg tactaatgat gtgtaactta
      481 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaatctgt
      541 agcaccaatc ataactatct gttttagcaa gcattaaccg cattcactaa gctatagttg
      601 atgacacaaa cagaaagttg atgcatgatg ttgccatata tatgtcacaa atacggcaca
      661 acgatcttca aacatatagc cacacattca cagaagagca caaagccttg caatccagtg
      721 caaagcttgg tgtgtctccg ttttgtttca tcagttacac ataactatat gatcattaac
      781 taaaagtcag tatacttgga gaaattttaa ttgcgtaata gagcatgcat atgattgaaa
      841 ttaacagttt attattaata acatccgtga tattgatgtc gttattagct gtggggttat
      901 ttgataaaat ttccccaata aatcttgttg agcacggcag gaataatcaa atagatggaa
      961 tgcgggggtt cttagcaatt ttcgtgctta ttcatcacgc agcaatttgg aatggatact
     1021 tgtcatctgg agtatgggaa gcaccttcat caaatctgtt agcaaattta ggccaagttg
     1081 gtgtgtcatt cttttttatg attactggtt atctgttctt ttcaaagatt atctctggag
     1141 atcaggactg gacaaggctt tatgtatcaa gattactacg attaacccca atgttcatag
     1201 ttagcctatg tctcattttt atcattgtag gttttaagtc tggatggaga atgcaggtat
     1261 ccacagaaga gctttttgtg tcaataatga agtggttgcc attcactgcg ctaggtatgc
     1321 cgaacattaa tgacgtaaaa gattcattta ctatcaatgc cgctgtaaca tggacacttg
     1381 tatatgaatg gttcttttat ttttctcttc cggtaatttc cgcgctcata aaaagaaaag
     1441 tcagtattta catggttatg attagcgcaa tatcgctatt tgttttcatt ttatttttca
     1501 gtaaaataca catagcatca tttctttttg ggctcctggc atttttgcta aataaatcaa
     1561 agatagtcaa tggaattgcc aaagcaaaag taacaccaat tataataact gcgataatgg
     1621 tttttgaaat gacgtatttc aaaacaactt acgcaccgct accactgatt ctttgtggaa
     1681 taacatttat cattatcgca tcaggttgtg atttgtatgg aatattaaga ttaaatataa
     1741 ccagaaagct aggagaaact acctacagcg tttaccttct gcatgggata ttcctttatt
     1801 gcctaatgac gtggattatt cctaataatt acactgaaaa tacatttatc atactggtaa
     1861 gcacaactgc atttcttatt acgttcacat catgcctaac ttttaaatta atagagacgc
     1921 cattcatcaa attaacaaaa caaaccacaa ctttagtaaa agaattaatg cccacattaa
     1981 caaacaacaa tcaataaaca ataaacataa gccgccctac atatatgtcc aaatggggcg
     2041 gcgaatgtat ttatattagt ctggtaatgc taaatgcaaa cgaactggag attcctgccc
     2101 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac gccaatataa gtaacatcac
     2161 aactaactga atcaggcgca gatataggtg ttatctgcac aatataaccg ctagcaactg
     2221 ctgcgagcgg atgagaaaag ctaacatcat acacacctgt agaaaccacg ttaaccgatg
     2281 aaacaccagt agcaacgtcg atactaccat tggcgttaat ttttcccgag cgtgtgactg
     2341 acaatgcaga agccgatgtt accttactgg ttgtttctgt aaaaacacac ccaatgtcaa
     2401 ctacctcgca attagctcct gttatccagt atggtcgttc tgcacttggc tgataatccc
     2461 ctgcgctctg gaacgttgtg ccgttgagta tgactgtaac ttttccccca cctgagcttg
     2521 ttacttgaat attcgtgtgc gtgtatcttg cactgctcac gcgatgaaaa tttccaccgt
     2581 ttataaccac agttacaggg cgcgtcccag tattatctat agcaaggtct gcccccccgg
     2641 cattagcctc aaagtatggg ttgtttaata ccaaagcgca cgaaccgtta aggccattta
     2701 tagctaattg cgcaccacct gtactggaat ccccctgagt accatttcct tcacaa
//
LOCUS       DAECOD010000210         2744 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.212, whole genome shotgun sequence.
ACCESSION   DAECOD010000210 DAECOD010000000
VERSION     DAECOD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.212"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..199
                     /locus_tag="KKO15_003969"
     CDS             <1..199
                     /locus_tag="KKO15_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBD6362558.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            238..1041
                     /gene="agaC"
                     /locus_tag="KKO15_003970"
     CDS             238..1041
                     /gene="agaC"
                     /locus_tag="KKO15_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBD6362559.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1031..1822
                     /gene="agaD"
                     /locus_tag="KKO15_003971"
     CDS             1031..1822
                     /gene="agaD"
                     /locus_tag="KKO15_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBD6362560.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1823..2578
                     /locus_tag="KKO15_003972"
     CDS             1823..2578
                     /locus_tag="KKO15_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBD6362561.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          684 a          634 c          678 g          748 t
ORIGIN      
        1 taaattggta gaaggtggca ttgacctgaa agatgtcaac gtcggcaata tgcatttctc
       61 ggaagggaaa aagcaaatca gcagtaaagt ttatgtcgat gaccaggatc tcgcggactt
      121 acgttttatt aaacaacgtg gcgtgaatgt tttcattcag gacgtccctg gcgatcaaaa
      181 agaacaaatc cctgactaaa tctaaaatcg ccttaatatt ggtttgaggt aataaaaatg
      241 catgaaataa ccctacttca gggattatcc ctagcggcgt tagttttcgt tctggggatt
      301 gatttttggc tggaagcctt atttttattc cgcccgataa tcgtttgtac cctaactggc
      361 gctattctcg gtgatattca gactggctta attaccggtg gtctgacaga gttggctttc
      421 gccggattaa cccctgcagg tggtgttcag ccgcccaacc cgattatggc gggtctgatg
      481 accaccgtca ttgcatggtc tacgggcgtt gatgccaaaa cagcaattgg tcttggcctg
      541 ccgtttagtt tgttaatgca gtacgtcatt ctgttcttct attccgcttt ctcattattt
      601 atgaccaaag ccgataaatg cgcgaaagag gcggatacgg cagcgttttc ccggcttaac
      661 tggacaacga tgctcatcgt cgcttcagcg tatgcggtga ttgctttcct ctgtacttac
      721 ctggcacaag gggcgatgca ggcgctggtg aaagcgatgc ccgcctggtt gacccacggc
      781 tttgaagtgg ctggcggtat tctgcctgcc gttggttttg gcttgctgct gcgcgtaatg
      841 ttcaaagcgc aatatatccc ttacctgatc gccggtttcc tgtttgtttg ctacatccgg
      901 gtcagcaacc tgttgccggt tgccgtactg ggcgcaggct ttgcggtgta tgagtttttc
      961 aatgcgaaat cccggcagca agcgcaaccg cagcccgttg ccagtaaaaa tgaagaagag
     1021 gactacagca atgggatctg aaatcagtaa aaaagatatc acccgtctgg gctttcgttc
     1081 gtcgctgctg caagcgagct ttaactacga aaggatgcag gcgggcggtt ttacctgggc
     1141 gatgttgccg atcctgaaaa agatttataa ggacgacaaa ccgggcttaa gcgcggcgat
     1201 gaaagataac ctcgaattta ttaataccca cccgaatctg gtcggattcc tgatggggtt
     1261 attaatttcg atggaagaaa aaggagaaaa ccgcgacacc attaaaggcc tcaaagtggc
     1321 actgtttggc ccaatcgccg ggattggcga tgcgattttc tggtttactt tgttgccgat
     1381 tatggcggga atttgctcat catttgccag ccagggaaac ctgctggggc cgattctatt
     1441 tttcgccgtt tacctgctta tctttttcct gcgcgtcggc tggacccacg tcggttattc
     1501 agtcggcgtg aaggcgatcg ataaagtgcg agagaactcg cagatgattg ctcgctcggc
     1561 aaccatcctc gggatcacgg taatcggcgg gctgatcgct tcgtatgtgc atattaacgt
     1621 ggtgacatcg tttgccatcg acagtaccca cagcgtcgcg ctgcaacagg atttcttcga
     1681 taaagtcttc ccgaacattt taccgatggc ctacaccctg ctgatgtatt acttcctgcg
     1741 ggtgaaaaaa gcgcatccgg tgctgttaat cggcgtgact tttgtgctct ctattgtttg
     1801 ttccgcattc ggcattttgt aaatggaacg aggcactgct tctggcggtg cctctttact
     1861 taaggaattt catcctgtgc aaacccttca gcaagttgaa aactatacgg cgttaagtga
     1921 acgagccagc gaatatttat tggctgtgat ccgtagcaaa ccggatgccg tgatttgcct
     1981 ggcgaccgga gccacgccat tactgacgta tcattatctg gtagaaaaaa tccaccagca
     2041 gcaggttgat atcagccagc tcaccttcgt gaagctcgac gaatgggtgg atctgccatt
     2101 aacgatgcct ggcacctgcg aaactttcct acaacagcat atcgtgcagc cgctggggct
     2161 acgtgaagac cagctcatca gctttcgctc cgaagagata aatgagacag agtgcgaacg
     2221 ggtaacgaac ctgattgcgc gcaaaggcgg tctggattta tgcgttctcg gattggggaa
     2281 aaacggtcat cttgggctga acgaaccggg agaaagccta caaccggcct gccatatcag
     2341 tcaacttgat gccagaacac agcaacatga gatgttaaaa accacgggtc gccccgtgac
     2401 tcgtgggatc accttaggcc tgaaggatat tctcaatgcc cgcgaggttt tgttattggt
     2461 gactggcgaa ggaaagcagg atgcgacaga acgttttctc acggctaaag tctccaccgc
     2521 tatcccggct tcatttttat ggctgcacag taactttatt tgtttaatta atacctaacc
     2581 agatgaaaaa tttgctcaaa atttttaaat taacttattc aacagtcacg cattatatta
     2641 aataacattt gactgggttg aacataacgc caatagcaaa aggagttatg ttcaacaatt
     2701 taattttaga tatacatata acttgttcta ttaataaatc acct
//
LOCUS       DAECOD010000211         2719 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.213, whole genome shotgun sequence.
ACCESSION   DAECOD010000211 DAECOD010000000
VERSION     DAECOD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.213"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="KKO15_003973"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="KKO15_003973"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBD6362562.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPSAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>2719
                     /locus_tag="KKO15_003974"
     CDS             1649..>2719
                     /locus_tag="KKO15_003974"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6362563.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEI"
BASE COUNT          701 a          615 c          712 g          691 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctag cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatc
//
LOCUS       DAECOD010000212         2716 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.214, whole genome shotgun sequence.
ACCESSION   DAECOD010000212 DAECOD010000000
VERSION     DAECOD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.214"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            52..2575
                     /locus_tag="KKO15_003975"
                     /pseudo
     CDS             52..2575
                     /locus_tag="KKO15_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2586..>2716
                     /locus_tag="KKO15_003976"
     CDS             2586..>2716
                     /locus_tag="KKO15_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6362564.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     "
BASE COUNT          727 a          585 c          681 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accactcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccggg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     2581 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     2641 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcacagta
     2701 cgcttgattt ctcggg
//
LOCUS       DAECOD010000213         2644 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.215, whole genome shotgun sequence.
ACCESSION   DAECOD010000213 DAECOD010000000
VERSION     DAECOD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.215"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..570
                     /locus_tag="KKO15_003977"
     CDS             <1..570
                     /locus_tag="KKO15_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD6362565.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KKO15_003978"
     CDS             571..936
                     /locus_tag="KKO15_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD6362566.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KKO15_003979"
     CDS             933..1616
                     /locus_tag="KKO15_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD6362567.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KKO15_003980"
     tRNA            1815..1890
                     /locus_tag="KKO15_003980"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KKO15_003981"
     tRNA            1892..1968
                     /locus_tag="KKO15_003981"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KKO15_003982"
     tRNA            1971..2046
                     /locus_tag="KKO15_003982"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KKO15_003983"
     tRNA            2050..2124
                     /locus_tag="KKO15_003983"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KKO15_003984"
     CDS             2417..2632
                     /locus_tag="KKO15_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6362568.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          692 a          582 c          714 g          656 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagatta
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcat
//
LOCUS       DAECOD010000214         2624 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.216, whole genome shotgun sequence.
ACCESSION   DAECOD010000214 DAECOD010000000
VERSION     DAECOD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.216"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            97..1071
                     /gene="cysB"
                     /locus_tag="KKO15_003985"
     CDS             97..1071
                     /gene="cysB"
                     /locus_tag="KKO15_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBD6362569.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1366..1530
                     /locus_tag="KKO15_003986"
     CDS             1366..1530
                     /locus_tag="KKO15_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBD6362570.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1533..1700
                     /locus_tag="KKO15_003987"
     CDS             1533..1700
                     /locus_tag="KKO15_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362571.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1814..1909
                     /locus_tag="KKO15_003988"
     CDS             1814..1909
                     /locus_tag="KKO15_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362572.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2072..>2624
                     /locus_tag="KKO15_003989"
     CDS             2072..>2624
                     /locus_tag="KKO15_003989"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBD6362573.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVN"
BASE COUNT          681 a          610 c          612 g          721 t
ORIGIN      
        1 atagttagca ccttttttat tattaaatcg tattagtcac ccgccaggtg tgacgaaaaa
       61 acgatgttct gatggcgtct aagtggatgg tttaacatga aattacaaca acttcgctat
      121 attgttgagg tggtcaatca taacctgaat gtctcatcaa cagcggaagg actttacaca
      181 tcacaacccg ggatcagtaa acaagtcaga atgctggaag acgagctagg cattcaaatt
      241 ttttcccgaa gcggcaagca cctgacgcag gtaacgccag caggacaaga aataattcgt
      301 atcgctcgcg aagtcctgtc gaaagtcgat gccataaaat cggttgccgg tgagcacacc
      361 tggccggata aaggctcgct gtatatcgcc accacgcata cccaggcacg ctacgcgtta
      421 ccaaacgtta tcaaaggctt tattgagcgt tatccccgcg tttctttgca tatgcaccag
      481 ggcccgccga cacaaattgc tgatgccgtc tctaaaggca atgccgattt cgctatcgcc
      541 acagaagcgc tgcatctgta tgaagattta gtgatgttac cgtgctacca ctggaatcgg
      601 gctattgtag tcactccgga tcacccgctg gcaggcaaaa aagccattac cattgaagaa
      661 ctggcgcaat atccgttggt gacatatacc ttcggcttta ccggacgttc agaactggat
      721 actgccttta accgcgcagg gttaacgccg cgtatcgttt tcacggcaac ggatgctgac
      781 gtcattaaaa cttacgtccg gttagggctg ggggtagggg tcattgccag catggcggtg
      841 gatccggtcg ccgatcccga ccttgtgcgc gttgatgctc acgatatctt cagccacagt
      901 acaaccaaaa ttggttttcg ccgtagtact tttttgcgca gttatatgta tgatttcatt
      961 cagcgttttg caccgcattt aacgcgtgat gtcgttgatg cggctgtcgc attgcgctcc
     1021 aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc cggaaaaata atttcttgcg
     1081 ttattttcgg caccttttat gtagcgaagg tgccggaata tattctcttt tgttacttat
     1141 ttctataccg tgcacaatat ttttatccct catatttata gggtaaatta cctgtaaagt
     1201 agtgtctaat tacccgcctc gcaaattcca gttttcaaat atctatttca ggtcaaaaga
     1261 ttgaattatt cactggagac gattcgtaaa ttcgctggat ttttgactaa agtttcttta
     1321 ggatttgtct catcgtatga ttaattacac caatcattca acgtcttgct gataagtgat
     1381 ggcgattata tgaggttagc aatgccttct ggaaatcagg aaccccgccg tgatcctgaa
     1441 ttaaaacgta aagcctggct ggcggttttt cttggttctg cacttttctg ggtggttgtc
     1501 gcactgctga tttggaaagt gtggggataa ctatggtcgg tcaggagcaa ctggagtcgt
     1561 caccattatg ccagcatagt gacaatgaac cagaaacgaa acgggaatgt tccgtcgtta
     1621 ttccagacga ctggcaacta acatcgcagc agcaagcctt tatagaactg tttgctgaag
     1681 atgatcagcc gaaacaataa ttatcatcat tcttattacc catttttaat gaattaaagg
     1741 gcttttaata caccgcagca ataacagctt gagttatctc aacacaaaat aataaccgtt
     1801 aagggtgtag cctatgatca acacaaatat gaaatattgg tcctggatgg gcgcgttttc
     1861 tctgtcgatg ctcttctggg ccgaactcct ctggatcatt actcactgat ccttgacccc
     1921 gctgcggcgg ggttgtcatt tgctttgcca caaggtttct cctcttttat caatttgggt
     1981 tgttatcaaa tcgttacgcg atgtttgtgt tatctttaaa ttcaccctga agagaatcag
     2041 ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa
     2101 ggacacgttg caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa
     2161 atcactgggc gatatcaccc gtctacccaa gtcactcaaa gttttgctcg aaaacctgct
     2221 gcgctggcag gatggtaact cagttaccga agaggatatc cacgcactgg cgggatggct
     2281 gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga
     2341 ctttaccggc gtacctgccg ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct
     2401 cggtggcgat actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc
     2461 ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat
     2521 ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga aagcaagcgt tcagtcggtt
     2581 tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt aacc
//
LOCUS       DAECOD010000215         2520 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.217, whole genome shotgun sequence.
ACCESSION   DAECOD010000215 DAECOD010000000
VERSION     DAECOD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.217"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..971)
                     /locus_tag="KKO15_003990"
     CDS             complement(<1..971)
                     /locus_tag="KKO15_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6362574.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANT"
     gene            complement(1571..1819)
                     /locus_tag="KKO15_003991"
     CDS             complement(1571..1819)
                     /locus_tag="KKO15_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD6362575.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2174..>2520)
                     /locus_tag="KKO15_003992"
     CDS             complement(2174..>2520)
                     /locus_tag="KKO15_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6362576.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          645 a          468 c          613 g          794 t
ORIGIN      
        1 aaggtgttgg cgtgctcttc atgttcaaaa gcatgccata tctgtgatac atcagattgt
       61 ttgttttccg ggaaccatgc tgtcacggca tcagccaggg ggcgatggag tgtattctgt
      121 tgtccgtcac tcatggagaa gtaaatctgc gggccgtggt agtccggcga agatgttaat
      181 ctttgcaggg attgcagagc atgggatgac aatgggttat cactaatatc tattgaacat
      241 tcattcctca gattaagaat gctttccggg atatgactta tctgatttat gtcaaaataa
      301 ccgttcctta aattttccgg caggtatgga atatcggtta acatattgtc gattgcccat
      361 atggagtgaa tgttgtgagg tagagggggg agttcactaa gaagattccc tattgcgcta
      421 atctcttgta aagaaaatgg taaaggtggt aaaactgcca gcccattaca tgatacatct
      481 aatgtttcca gtagttctgg taaaacagga agagaacata attgattatt tgagacatga
      541 agctccttca aagatatggg gagtgtgggt agtgtgatta gttgattgtg ggacgcattc
      601 aataatttaa gtccttgagg caaagcaggc agttcaataa gtctgttata gctgacatta
      661 agttgtgtaa gggacgcagg caatggggag attaagctta aattattttt acttatatta
      721 attgatttaa ttcccggggg gatttcaggt aatgttgtca ggcctaattc agacaagtcc
      781 aggttcgtct cttggttttg tagacatgat actagtcgct gaaaagcgat gtctcgttgt
      841 tcttcttgta tgcggttatt tttccattca gtccaatggg ttagataatt ttcatatgcg
      901 cggctagtgt cgattgaata agtggagaac gaatttgaaa ttaatctgtg attgttattt
      961 gtcgggagca taaatatcag ggcgtattct ttatgttgag aggtgttgga ttctttttta
     1021 tctgctttgc aatgttattg gttcccttca gcagtagaca ggaacttctg gaggaggcgg
     1081 atatatagtt tgtgtaagca tattaactcc atatgttata tattgaagaa cttctgcttt
     1141 acactatcct aaactggatc ggtttaatgt aaaaaaacga tacttttttg ggggggggca
     1201 atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg
     1261 gtatcagtga atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt
     1321 tattcttctg gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt
     1381 gttttgctga gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca
     1441 tgattggcgg gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc
     1501 ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt
     1561 tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt
     1621 cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt
     1681 tgctggtggc ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta
     1741 attcttcctt taacgcatcc acagcaccgg ttggcatttt agtcattttc tctttggcta
     1801 tgcagatttc aaaacgcata aaaatccctc tacactgtgt ttttatacag tattattttt
     1861 aactgtatgg ataaacagtg tcaagagtga gggaaagtga ttttttgggg catgcatggg
     1921 gcaaaaatat ttgttgtggg gcattttggg gcatgattgg gacactttgt tacatatgaa
     1981 ctttgccgat tttcatatga catggcaatc caacgctttg attattctaa agaaaacaca
     2041 tgctcttggg cgttctttag tgatttttaa aaatcccgta tcacgcagtt aaagtggcgg
     2101 gcatactctt caaggctggt gatccccaac cgcacccatt taggatgtga ccattgcggt
     2161 agaccgatgt aaatcataac gcgtgaagga tctcttccac gctacgcaca cgggcgatgc
     2221 gcgggtagat atgattaatg ctgttattgt gttgctcggc gctggcggca ctacaggcgt
     2281 cttcggcaat caccagatta aagccaagtt cccaggcatt gcgggcggtg gattcaacac
     2341 cgatattggt cgagatccca cataacacta ttgtatcgat acctcggcgg cgcagttgca
     2401 actccagatc cgtaccgtaa aacgcacccc actgacgttt gatgatttcg atatcactgt
     2461 cagttgcacc taatgcagct ggatgttgcc accagttttc gggcaacact ttagcggggg
//
LOCUS       DAECOD010000216         2482 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.218, whole genome shotgun sequence.
ACCESSION   DAECOD010000216 DAECOD010000000
VERSION     DAECOD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.218"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..567
                     /locus_tag="KKO15_003993"
     CDS             <1..567
                     /locus_tag="KKO15_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD6362577.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="KKO15_003994"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="KKO15_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBD6362578.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="KKO15_003995"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="KKO15_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBD6362579.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="KKO15_003996"
     CDS             2169..2405
                     /locus_tag="KKO15_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBD6362580.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          636 a          545 c          642 g          659 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgcagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca attgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta ag
//
LOCUS       DAECOD010000217         2419 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.219, whole genome shotgun sequence.
ACCESSION   DAECOD010000217 DAECOD010000000
VERSION     DAECOD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.219"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..244
                     /locus_tag="KKO15_003997"
     CDS             <1..244
                     /locus_tag="KKO15_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD6362581.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(333..845)
                     /locus_tag="KKO15_003998"
     CDS             complement(333..845)
                     /locus_tag="KKO15_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBD6362582.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1040..1804
                     /locus_tag="KKO15_003999"
     CDS             1040..1804
                     /locus_tag="KKO15_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362583.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1755..>2419
                     /locus_tag="KKO15_004000"
     CDS             1755..>2419
                     /locus_tag="KKO15_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBD6362584.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDCFKHVYEGVPESD
                     DDAA"
BASE COUNT          682 a          540 c          613 g          584 t
ORIGIN      
        1 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
       61 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
      121 caccgtcgaa ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat
      181 cagcgaccag acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg
      241 gtagcggtaa ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga
      301 agctggattc gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta
      361 aaactaccaa cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact
      421 ttcactggat gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg
      481 ataaagcgcg aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt
      541 tctattgtat tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt
      601 ataccaagat ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca
      661 gcttcccatg gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat
      721 gctggtggca atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga
      781 agcccccata ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc
      841 atcaatgtct ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt
      901 aggcttaaac accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg
      961 caggagtgag tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta
     1021 aatcacagga agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg
     1081 ggccggagtc cttagcctcc gtgatatagg cgagaaatac ggcgttacag aaggggctat
     1141 caggaagagg gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg tttgcaaaaa
     1201 tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca
     1261 aaaaagtacg caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg
     1321 taccgatccc ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg
     1381 ttatgcccgt cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct
     1441 cgaggacgaa ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg
     1501 gtggttggtg tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa
     1561 tatcagcgcc gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg
     1621 cacgcttgcg acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga
     1681 taaggtgtct ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg
     1741 agagcgtgac ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac
     1801 gtagtttctg gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa
     1861 aatcatggga tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt
     1921 gttgtgcccg tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc
     1981 agatagacag gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc
     2041 gggttactgg ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt
     2101 cactggaggg gatcgatatg ttgtggctgg aagaagccca cgcactgacg gaataccagt
     2161 ggaaaattct ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc
     2221 ccggacttgt tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc
     2281 tcatccgcaa aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta
     2341 tcgacgcggc gcgacgccgt gatccggatt gttttaaaca tgtgtatgag ggcgttccgg
     2401 agtctgatga tgatgcggc
//
LOCUS       DAECOD010000218         2395 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.220, whole genome shotgun sequence.
ACCESSION   DAECOD010000218 DAECOD010000000
VERSION     DAECOD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.220"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(729..803)
                     /locus_tag="KKO15_004001"
     tRNA            complement(729..803)
                     /locus_tag="KKO15_004001"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:Gly,seq:tcc)
     gene            complement(807..882)
                     /locus_tag="KKO15_004002"
     tRNA            complement(807..882)
                     /locus_tag="KKO15_004002"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Thr,seq:tgt)
     gene            complement(885..961)
                     /locus_tag="KKO15_004003"
     tRNA            complement(885..961)
                     /locus_tag="KKO15_004003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Arg,seq:tct)
     gene            complement(963..1038)
                     /locus_tag="KKO15_004004"
     tRNA            complement(963..1038)
                     /locus_tag="KKO15_004004"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Ile,seq:cat)
     gene            complement(1215..1904)
                     /locus_tag="KKO15_004005"
     CDS             complement(1215..1904)
                     /locus_tag="KKO15_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD6362585.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1901..2260)
                     /locus_tag="KKO15_004006"
     CDS             complement(1901..2260)
                     /locus_tag="KKO15_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD6362586.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(2260..>2395)
                     /locus_tag="KKO15_004007"
     CDS             complement(2260..>2395)
                     /locus_tag="KKO15_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362587.1"
                     /translation="VPALRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR
                     "
BASE COUNT          620 a          650 c          507 g          618 t
ORIGIN      
        1 ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg ggcccccccc
       61 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat gacctgaatc
      121 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      181 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      241 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      301 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      361 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      421 cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      481 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      541 tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat
      601 tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg
      661 gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca
      721 aatctcgctg gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt
      781 aatagccatt atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta
      841 ctgattacaa gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg
      901 actcgaacct cggaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg
      961 gttggtggcc cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
     1021 ccactgagct aaagggccgg aggcagaata ataaccatat gtcatcacat ctgcaaactc
     1081 atctgaccac cagcacgttt aacgtcctgt gccatttttc aggcataaaa aaaccgctcg
     1141 gcggcgggtt ttgcttactt taccatcgcg tacaaaatcg gcaaagtatc agatttgcat
     1201 gagatatatg ccttttaatc tacttttgca atactttgct ttgaaaatgc cgctttttgt
     1261 tctgaacgtg ttctcatcac aaacaataaa gcctcactat ccagtcggtg aaaaatgtgt
     1321 ttcattacaa cccagtgacc agtaaatgtt ttggaccagt ttttggttgt tactcccgcc
     1381 agtaatgcca gctcctgata ttcataacct tccccaccaa aaagttctgc ttttaccgcc
     1441 tgcgccgcca gccagattaa ttttttcagg cgttcctgtg ttttctctgc aattttccta
     1501 gtgccagaat gagccttaaa ctcttcccac gcccagtggg ttatggtgat ctgatactcc
     1561 cagcaaatac ttccgctgta acaccacaac aaccaggctt tatagtgctc ttcaagagac
     1621 agaacagccc gccgccacga tgatgtcgaa aacgcaacct gactgaccag aggaattgac
     1681 gcccccttcg ccagtgattg ttttcccggg attggtggat tatcccgcgt tatcattttt
     1741 ccggtcacct catcgcggta ccggattttt ttacgcctgt aacgccctgt atcgaacatg
     1801 gcattctcct gccaggcttc aagctgacct tttgtcgccc cactcaaatc agcggtggcg
     1861 ataatgagct gctcacgaac aaactgtaaa tactggttat tcatgcacac tccagctctg
     1921 tgatttttat ccccaaccga ccaccaggaa caggcagtcc gcgcacaata ttggtttcat
     1981 caaactgctc gtcgtcgata agcaaccccg catgtgtcag tgcatccagt ggtgctttca
     2041 ggatattgtc caggtcacga cgccgcttat ccggtggctc tgcaataatc tttatcgcca
     2101 gtcttccgga caggcttaat ttcagtcgct gctgacggac aataagcgcc acatcccggc
     2161 gataacgctc accggctttt gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat
     2221 tcaccgtcgg cggataaggt aaaataaact catggcgcat cagcgcagca cctcctgcac
     2281 cagcttttca aactttccga ctctggtttc cagctctgcc acacagtcca ccagctcatc
     2341 tactgctttc tgtgcgcgat gcttcgcctg catcagttcc cgaagcgcgg gtacc
//
LOCUS       DAECOD010000219         2389 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.221, whole genome shotgun sequence.
ACCESSION   DAECOD010000219 DAECOD010000000
VERSION     DAECOD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.221"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..107
                     /locus_tag="KKO15_004008"
     CDS             <1..107
                     /locus_tag="KKO15_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBD6362588.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="KKO15_004009"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="KKO15_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBD6362589.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="KKO15_004010"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="KKO15_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBD6362590.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2389
                     /locus_tag="KKO15_004011"
     CDS             2210..>2389
                     /locus_tag="KKO15_004011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBD6362591.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          652 c          673 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtgg
//
LOCUS       DAECOD010000220         2266 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.222, whole genome shotgun sequence.
ACCESSION   DAECOD010000220 DAECOD010000000
VERSION     DAECOD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.222"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1082)
                     /gene="ipaH3"
                     /locus_tag="KKO15_004012"
     CDS             complement(<1..1082)
                     /gene="ipaH3"
                     /locus_tag="KKO15_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBD6362592.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     HRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQ
                     VAAWLEKLSTSAELRQQSFAVAADAT"
     gene            1824..>2266
                     /locus_tag="KKO15_004013"
     CDS             1824..>2266
                     /locus_tag="KKO15_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBD6362593.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          625 a          408 c          570 g          663 t
ORIGIN      
        1 tcagtggcat cagcagcaac agcgaaagac tgctgtcgaa gctctgcaga ggtactgagt
       61 ttttccagcc atgcagcgac ctgttcacgg aatccggagg tattgcgtgc agagacggta
      121 tcggaaaggc ggtcaaggaa cgcggaaaag gtgttggcgt gctcttcatg ttcaaaagca
      181 tgccatatct gtgatacatc agattgtttg ttttccggga accatgctgt cacggcatca
      241 gccagggggc gatggagtgt attctgttgt ccgtcactca tggagaagta aatccgtggg
      301 ccgtggtagt ccggttgggc ggtttgttgc gacagagact ccctgatccg tgaggacaga
      361 ggattgtctt cgaggatgat agtgcaggtc ggatcaaggc taagtatatt ttccggaatg
      421 tgtgtgatgc gattctcgcg gcaccggaaa aatatctcgg tttcctctga gtgatgattt
      481 cttacaggta cggctggtag gctttccaga agattagtac ttacatcgag cgcttccagt
      541 gattcaggta actcagggag aaatgtcagc tggttatttc ttactgagag cacttccagc
      601 gatgtaggta attcaggaag catggttagc tgattgttat ctgcattaat atattccagc
      661 aatgcaggca attcaggaag catggttagt tggttgttat ctacatcaag atgtttcaga
      721 gatgcgggta attcaggaag tgttgacagg cgattgtcac aggcgtcaag gtattccagc
      781 gatgctggca attctggtaa tgatattagg gcattctgag taatttccag aacagtgatt
      841 tgaggtggta agttgtcagg tagcgaggac agatttaaac gattcagttg aagctcactg
      901 aactgattga tgagacattc tttaagtagg gagaccgctt cattccgatt ttcaccgggg
      961 agcgcttgtt tttcccattt atcccatgct gaaaagtaat cagcatatgt accggaaata
     1021 gtgttataaa aagaattttg ggacaatgaa aagttattat ttatcggtaa catgatggac
     1081 attcagttaa agccgatgga caaatggctt tgttaaaaga aataagtagg agtttttatg
     1141 aagttcattc catgaaatga acttcagcac ttttttatta taaaaggaca aacaaaattg
     1201 gctgtaaaaa aacgatactg tttcagttaa ctgtgaatag cttgcttgca ccgtattgtt
     1261 cttgtttttc ggttagttaa acatcgtact tcatatttga acgttctgcc ggaatgcatt
     1321 atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa accgtagcac gtcgtatgca
     1381 agaacgtgcc acggctggct gatggatgtt cgatagcgcg agtttgaatg aaaatcagcc
     1441 ggagatgatt ttacataatt gctacggaat tattcaatac aggaattgct tgcgtctgca
     1501 tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa ctcgaaaaaa atggtaactg
     1561 gttgaatgta ttaatatgca atggtacgtg tcagggatta aaagatgaac gtaaatttat
     1621 tcaacgcatt aattttaaag ggttttattg tttgttgacg aaaacaggaa tcatgttcgg
     1681 tctcttttta tctgttaaaa gccagaagca tttccttcgc tgactttata gtcaaccata
     1741 acacacactc tactgtctga gtccagcgtt ttttaacatt cttgttaaga ttatgtgatc
     1801 tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc cgtttatcag agaattgcgg
     1861 gtcatcaatg gcgacatatc tggctttctg gcgatataca cggttgtctt gagcagttgc
     1921 gccgcaaatt atggcattgt cgttttgatc cgtggcgaga tttacttatc tcagtgggag
     1981 acgttatcga tcgtgggccg caaagtttac gttgtctgca gttactggaa caacattggg
     2041 ttcgtgcggt aagaggcaat catgaacaga tggcgatgga tgcgctggca ttccagcaga
     2101 tgtctttgtg gttgatgaat ggcggcgact ggtttattgc gctggcagat aatcaacaga
     2161 aacaagcgaa aacggcgctg gaaaaatgtc agcatttgcc ctttattctt gaagtacaca
     2221 gtcgcaccgg caagcatgtt attgctcatg ccgattatcc agatga
//
LOCUS       DAECOD010000221         2208 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.223, whole genome shotgun sequence.
ACCESSION   DAECOD010000221 DAECOD010000000
VERSION     DAECOD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.223"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..768
                     /gene="waaO"
                     /locus_tag="KKO15_004014"
                     /gene_synonym="rfaI"
     CDS             <1..768
                     /gene="waaO"
                     /locus_tag="KKO15_004014"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBD6362594.1"
                     /translation="YNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYHKHEK
                     ILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGYFNA
                     GFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR
                     YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLL
                     KPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF"
     gene            794..1501
                     /gene="rfaY"
                     /locus_tag="KKO15_004015"
     CDS             794..1501
                     /gene="rfaY"
                     /locus_tag="KKO15_004015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBD6362595.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            1526..>2208
                     /locus_tag="KKO15_004016"
     CDS             1526..>2208
                     /locus_tag="KKO15_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBD6362596.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLH"
BASE COUNT          794 a          301 c          393 g          720 t
ORIGIN      
        1 tataattcaa gaattaatat ttatgttatc aattgtgata agctgaagtc attaccaagc
       61 acgaaaaact ggacttacgc aacatatttt cgatttataa ttgcagatta tttctatcat
      121 aaacatgaaa aaatactata tcttgatgca gatattgctt gcaagggtag tattaaagaa
      181 ctcttagatt atcaattttc tactaatgaa attgccgcag ttgtagctga aagagatatt
      241 gaatggtggc aaaatcgagc ctcggtatta actacaccac agttagcttc tggatatttc
      301 aatgctggtt ttttactgat aaatattgat gagtggaatc taaataacat ttcgtcaaaa
      361 gctattgaaa tgttgcgtga cccagattgg gtaagtaaaa tcacccacct tgatcaagat
      421 gtactgaatg tattattgaa tggtaaagtg aagtttattt cggagaaata taatacccga
      481 tatagtatta actatgaatt aaaagacaaa gttgataatc cagtcaatga tgacaccgtg
      541 tttatacact atgtgggacc tacaaaaccc tggcatgagt gggctgatta tccggtttca
      601 cgtagttttt tgatcgccaa agcagcttct ccgtggagta aagaagattt acttaaacct
      661 gtaaatagca atcagtatcg gtattgtgca aaacataaat ttaaacaaaa gcattatatg
      721 gcaggcattt ttaattattt aaagtattat aaagaaaaat gcttctaaag atttaaggtt
      781 aaaataattc attatgatat ataataaaac gataaatggg ttgaaagttt tcataaaaga
      841 taatgatccc ttttatgagc aagttctaaa tgacttttta acttgcaggg ttaaaacatt
      901 aaaagttttt cgttctattg atgatactaa agtcattctt attgacactg ctcgtggacc
      961 tcttgtactt aaagtttatg cgccaaagca taaaatgacc gaacgtttct taaaatcttg
     1021 cataaaaaaa gattattatg aaaatttaat ttatcaaacc gatcgggtac gtggtgaagg
     1081 catccaatca attaatgact attttttgct agctgaacgt aaaacattaa atttcgctca
     1141 ttattatatt atgctcattg agtatattga aggtgtcggg ttaaatgagt atttggaaat
     1201 ttctgaagat ctgaaggatc aattatcaga atcgataaaa gagttgcatc agcatggaat
     1261 ggtgtctggc gatcctcata aaggaaattt tatagtatcc gaaaaaggtt taagactaat
     1321 tgacctttca ggtaaaaaaa caaccgctgt tttaaaagca aaagatcgca ttgatcttga
     1381 acggcactat aatataaaaa atgaattgaa agactttggc tatacatatc ttatattcaa
     1441 aaagaaaatt aaaaaagtaa tcagggatgt aaaagtcaaa ctaggattga agagcaaata
     1501 atgattatga tgaagggtaa ttaaattgga ttttaaacat cttactcaat ttaaagatat
     1561 aattgaactg gacaagcgcc ccgttaaact tgatgaacgg gaaacgttta atgtctcatg
     1621 gggtattgat gagaactacc aggttggggc tgcgatttca attgcttcaa ttcttgaaaa
     1681 gaataaacaa aacaaattta cctttcacat aatcgctgat tacttagaca aagagtatat
     1741 tgaattatta tcacaattag caacgaagta tcaaacagta attaaattat atcatattga
     1801 ttctgagcca ttgaaggcgc tacctcaatc aaatatctgg ccagtatcta tttattatcg
     1861 tttgctttca tttgattatt tttctgcgcg attggattca ttattatatc ttgatgctga
     1921 tatcgtctgt aagggttcat tgaacgagtt aatagcatta gagtttaaag atgaatatgg
     1981 ggcagtggta attgatgtag atgctatgca aagtaaaagc gctgagcgtt tgtgtaatga
     2041 ggattttaac ggtagctatt ttaactctgg tgtaatgtat attaatttac gggaatggtt
     2101 aaaacaaaga ctaacggaaa aattctttga tctattatca gatgagtcaa ttataaaaaa
     2161 attaaagtac ccggatcaag atattttaaa cttaatgttt ctacatca
//
LOCUS       DAECOD010000222         2187 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.224, whole genome shotgun sequence.
ACCESSION   DAECOD010000222 DAECOD010000000
VERSION     DAECOD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.224"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..698
                     /locus_tag="KKO15_004017"
     CDS             <1..698
                     /locus_tag="KKO15_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362597.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="KKO15_004018"
     CDS             700..1305
                     /locus_tag="KKO15_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD6362598.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>2187
                     /locus_tag="KKO15_004019"
     CDS             1292..>2187
                     /locus_tag="KKO15_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6362599.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          508 c          610 g          537 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagtcg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg
     1981 cccgttcacc attaatttgc ggacgggtga aatatccatg tcgcataaag tgtctgttgg
     2041 cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa
     2101 ttcaattgtt cttggtgata atgacaccgg atttaaacag aatggagacg gtattctgga
     2161 tgtttatgct aatagtcagc gtgtatt
//
LOCUS       DAECOD010000223         2125 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.225, whole genome shotgun sequence.
ACCESSION   DAECOD010000223 DAECOD010000000
VERSION     DAECOD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.225"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            43..222
                     /locus_tag="KKO15_004020"
     CDS             43..222
                     /locus_tag="KKO15_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362600.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="KKO15_004021"
     CDS             274..468
                     /locus_tag="KKO15_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362601.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="KKO15_004022"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="KKO15_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          570 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ataacggctt taattggaat acatatatta caacgtatta tatataattg
     1801 gtattctggg aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag
     1861 gacagtggga ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc
     1921 ggagggaata ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa
     1981 atagttagcg ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt
     2041 atgcatgacc tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac
     2101 aaacacaata ttcatagtat ggcga
//
LOCUS       DAECOD010000224         2123 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.226, whole genome shotgun sequence.
ACCESSION   DAECOD010000224 DAECOD010000000
VERSION     DAECOD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.226"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..241
                     /locus_tag="KKO15_004023"
     CDS             <1..241
                     /locus_tag="KKO15_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362602.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KKO15_004024"
     CDS             complement(238..1179)
                     /locus_tag="KKO15_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD6362603.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KKO15_004025"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KKO15_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..>2123)
                     /locus_tag="KKO15_004026"
     CDS             complement(1684..>2123)
                     /locus_tag="KKO15_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362604.1"
                     /translation="KTLLKALLAKLERNATGDNDNKPDDNPTGDGEDDEEETTITGDS
                     AWRAEVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNA
                     VSEVAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          458 a          588 c          543 g          534 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tga
//
LOCUS       DAECOD010000225         2034 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.227, whole genome shotgun sequence.
ACCESSION   DAECOD010000225 DAECOD010000000
VERSION     DAECOD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.227"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>2034
                     /gene="sat"
                     /locus_tag="KKO15_004027"
     CDS             <1..>2034
                     /gene="sat"
                     /locus_tag="KKO15_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBD6362605.1"
                     /translation="YGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGI
                     ASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQE
                     KITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESI
                     VNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKV
                     ILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLA
                     INNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGH
                     ATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWK
                     TGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFD
                     FKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIA
                     QNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVT
                     GNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNA
                     IWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          746 a          358 c          401 g          529 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg tagcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agggatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaacg aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgcaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agat
//
LOCUS       DAECOD010000226         2033 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.228, whole genome shotgun sequence.
ACCESSION   DAECOD010000226 DAECOD010000000
VERSION     DAECOD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.228"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..363)
                     /locus_tag="KKO15_004028"
     CDS             complement(<1..363)
                     /locus_tag="KKO15_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6362606.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KKO15_004029"
     CDS             complement(430..1644)
                     /locus_tag="KKO15_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBD6362607.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTLIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          635 a          494 c          411 g          493 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcag cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggtcacca gaa
//
LOCUS       DAECOD010000227         2028 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.229, whole genome shotgun sequence.
ACCESSION   DAECOD010000227 DAECOD010000000
VERSION     DAECOD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.229"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..484
                     /locus_tag="KKO15_004030"
     CDS             <1..484
                     /locus_tag="KKO15_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination enzyme"
                     /protein_id="HBD6362608.1"
                     /translation="KKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQK
                     RLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER
                     "
     gene            complement(548..988)
                     /locus_tag="KKO15_004031"
     CDS             complement(548..988)
                     /locus_tag="KKO15_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD6362609.1"
                     /translation="MSKDTQRITQINDQGEIVGGFVAVIRPKQKSSFQRHFTMNQDAL
                     KILAKELTGEQFKVLMLMLADLDYENFIQIAQADIADALEMQKTHVSRAVRALLDVGV
                     IFEGPKVGRSKTYRLNEQFGWKGTVSNHKKALKNGLSVIQGGRT"
     gene            1544..>2028
                     /locus_tag="KKO15_004032"
     CDS             1544..>2028
                     /locus_tag="KKO15_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBD6362610.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYC
                     SVSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQW
                     LEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAE
                     SV"
BASE COUNT          497 a          498 c          564 g          469 t
ORIGIN      
        1 aaaaaaactg ggtcttgagc gcggcatcga gggaagccgc gccacgcatc agacagttca
       61 gcactactac gaatcgataa accgtggcac ccgtagccag gtatcgatct cgccggaggc
      121 gctggagccg cgtgtattgc ggaagggcat tttcaccaaa gacgtggaag atcaggcagc
      181 catagccaaa cgtctaagcc acgcagtgaa cgatggcttt gcaggaacca tcgctatggc
      241 ctctcagagc gcccagaacg caaaaagagc gcgtgagcta cagaagacta tggatgcgca
      301 gcagaaacgc ctacagagcg ttacagagcc ttttaagggg ctttccagag agcagatgac
      361 cgaaatcctg atgatggctc agcggtttaa gcagcaaaac caggaaaagg aaaaacagca
      421 gcgtattgaa cgtgaaaaac agcgacagac gcgatcgcgt ggcatgggtg gtatggagcg
      481 ttagttttga tgcgatgcag gatagggcta acgccgcaaa gtgacgtcat ttgcggcatt
      541 ccagagatca ggttctgccg ccctgtataa cgctgaggcc gtttttaagg gcttttttgt
      601 gattgcttac tgttcctttc cagccgaatt gctcgttcaa ccgataggtt ttggatctac
      661 ctactttcgg accttcaaag atcacgccta catcaagaag agcacgaaca gcgcggctta
      721 cgtgggtctt ttgcatctcc aatgcatccg ctatatcagc ctgtgcaatt tggatgaaat
      781 tctcgtaatc cagatcagcg agcatgagca ttagaacttt aaattgctcg cccgttagct
      841 ccttagcgag gatctttaat gcgtcttggt tcatagtgaa atgcctctga aatgacgatt
      901 tttgcttggg tctgattacc gcgacgaagc cgccaacaat ctcaccttga tcgttaatct
      961 gggtgattct ctgggtgtcc ttgctcatcg aaaataacct atcttgagtt accattattg
     1021 gtaacagtgt taccattatt ggtaactatg tcaaatgtaa cccctcgcca tacatacgtt
     1081 tttttatcgc tccttctgta tcttataacg cgtgcgcgcg gtttagctct gaataccaac
     1141 gttttttagc tacggagtgt acggatttct atgtacgggg agtggaaatt ccgcttcctc
     1201 gctcattcgc tcactacgtt cggtcatgag tctgcggcga gcggttcctg ctctttcaaa
     1261 agcgatattc attgcctgat ttcgtcaagg gcgagcgaag cgagtgccat ttacccttga
     1321 cgacactccg gataaaacag gcttggcagt ggtgggggtg gcgcggagcg acgccttcca
     1381 cctgccagac cgccgttgat tttgaccttc tggcagcttg ctgcccgaac gattttgcga
     1441 agcaaagtct agtgagtaca ctatcatctt gatagtggcc cgccggaggt atcgcaatgc
     1501 gctcccccgc ctggctgaat gaatgccgga atcagggggg aacatggcga actacgcgat
     1561 aatgcgctgc aaaaagctga cgggaatggg aagcgtggcc agcgcgttgc agcactgcta
     1621 ccgagagcgt gagacgccga acgctaacgc tgaacggaca ccagaaaact actgctctgt
     1681 gtccaaatca acggatcagg caatggggcg tgtgcgggag ctgctgccgg aaaaacgccg
     1741 taaagatgcg gtactggccg ttgaatacgt tatgacagca agccctgagt ggtggaaaga
     1801 ggccacacca cggcagcagg cggagttttt cgcccgctct gagcaatggc tggagaagaa
     1861 atacgggaag gatcgtgttg tggcggcggt ggttcaccgg gacgaggcca ccccgcacct
     1921 gtctgcgttc gtggtgccat tgacgcagga tggacggctg tccgcgaaag agtttattgg
     1981 cgggcggtca aagatgcgtg aagatcagag cacctacgct gaatcagt
//
LOCUS       DAECOD010000228         1982 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.230, whole genome shotgun sequence.
ACCESSION   DAECOD010000228 DAECOD010000000
VERSION     DAECOD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.230"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(109..1098)
                     /locus_tag="KKO15_004033"
     CDS             complement(109..1098)
                     /locus_tag="KKO15_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBD6362611.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGV
                     RMASCGGLLRFGWSTLKETGEMRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYNPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          415 a          595 c          535 g          437 t
ORIGIN      
        1 aaaaaaccga gaaacggcgg gaattttcgg gacaggatgt gggaatgtcg gaccgcgcac
       61 gctcgctcag acattcccca cctggtcagg gcttcgcccg acaccccgct agcctgattc
      121 agttttatct ttcgtcggtg agcgacggta ctttctgacc tcggttttcc actcgaacgc
      181 gatgcgtgaa ccgtcatcgt cgccctcgga aatatcatcc ccgatcacca tgtcggcgtc
      241 ggtttcctga tcgagtttca gcacgtcttt gagtgcgccg ccggtcgcca caaagcggcg
      301 cttgtgcgtc tggcgggtca gttcgaggaa ccattcagga tcggccacca tgtcagccgg
      361 cttcgtggaa tatttcaggg tttcggccac tgcgccgcgc accagttcag cggtcgcgca
      421 ggctaaggac tcgccatcct ttggcttacg gggttttact gcccggacat caacattcgg
      481 gttgtaactg acccgcaggc actcccgcca gagttcaacc cagcgagcat gttttacgta
      541 cccgtcacca gatagcattg agggcggcac catcatcagg gtgtggaagt ggggatgcgc
      601 gctgccgtcg ctgccgcggg taacttccgt tgtgcggatc cagccctgaa caggcaggaa
      661 ctccttgcga accttcatac gctgaaaagc tgcattcatc cggttcagca tctcgcccag
      721 ttccccaatc gcacagttgc gcacggtcag ggtcaggaac atccagcggg catcagggta
      781 atccgccacg atacgcggca gggactggta gaaacgggct tgccacatca gggagcgcct
      841 ccactggcag acaggacagt gccggacacg gcagaaatgc gcctcacgca gcctcagacg
      901 catttctccg gtttccttga gggtagacca cccgaaacgc agtaaaccgc cacaggaggc
      961 catacgaacc ccgtagcgct catattcggc cgcaagcagg taaatccctc ccacgtcgtc
     1021 agaaacgctt ctgtgtgcgt cccagggcgc gtcagagggg gaataatcag tgaggtactg
     1081 gagtttaggg tttttcatgc gcaccacccg attgtgggcg atggctcatc tgacgtgtta
     1141 tcattgccat atcacctgtg ttgtcgtgtc actaattcga atttctacag gtggtcacgc
     1201 ccccgaacga ttgccctcgt tgcgggggtc gcttttaacg cgaccacttc cgacagaata
     1261 cttctaaacc catttcggag caaatgtgcc ctcaagcctt gcgggacaag ggctggaggc
     1321 caatttgaat cctagagtta tcccttaagt ataaatacaa ggatcgcgcg cgagatttgc
     1381 ttgaattttg agctggattt tcagtaaccc aaaacctccc aaccccaccc acttacaccc
     1441 atctcatcca cagactttca ccccaatttt gtgaacaaca aaaaaccgtc ttgcaggtgg
     1501 ttggcctcac tccgtgacac caaccaccat acccagcgtt ttccccgtgc cgtcgtcaat
     1561 cacaaaatcg gaacccacgc cggaggcgcg gggccccgcg attttctgtt tgacgagttc
     1621 aagaacggtg agcggtacgc ggaatttttt cgcgtgccgt gaggcgttgg cggggtgtcg
     1681 gggcgaagcc ctgaccaggt ggggaatgtc tgagcgagcg tgcgcggtcc gacattccca
     1741 catcctgtcc cgatttctgc gggtttgtgt caccggaaca ctggcgcaat gtcagcagtt
     1801 ttttgcgccg cgccgccctc cacgtaagcg cgcagttctg gcaaccggac agcaaatcat
     1861 tctgccgttt tcgtgccaca gctgttccgc gcgatcgaca aactggcagt cggggaatct
     1921 cacgaagtgc gcactcatac aaactacact tcgtttcgtt tgtcgtgcgg tctgcgacgc
     1981 tc
//
LOCUS       DAECOD010000229         1846 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.231, whole genome shotgun sequence.
ACCESSION   DAECOD010000229 DAECOD010000000
VERSION     DAECOD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.231"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..254)
                     /locus_tag="KKO15_004034"
     CDS             complement(<1..254)
                     /locus_tag="KKO15_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362612.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="KKO15_004035"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="KKO15_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBD6362613.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="KKO15_004036"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="KKO15_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBD6362614.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="KKO15_004037"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="KKO15_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6362615.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /locus_tag="KKO15_004038"
     CDS             1250..1501
                     /locus_tag="KKO15_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362616.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="KKO15_004039"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="KKO15_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          463 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcacta aacccgcttc ggcgggtttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAECOD010000230         1833 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.232, whole genome shotgun sequence.
ACCESSION   DAECOD010000230 DAECOD010000000
VERSION     DAECOD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.232"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..180)
                     /locus_tag="KKO15_004040"
     CDS             complement(<1..180)
                     /locus_tag="KKO15_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD6362617.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="KKO15_004041"
     CDS             complement(321..875)
                     /locus_tag="KKO15_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD6362618.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="KKO15_004042"
     CDS             complement(872..1162)
                     /locus_tag="KKO15_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD6362619.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="KKO15_004043"
     CDS             complement(1162..1761)
                     /locus_tag="KKO15_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD6362620.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tac
//
LOCUS       DAECOD010000231         1819 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.233, whole genome shotgun sequence.
ACCESSION   DAECOD010000231 DAECOD010000000
VERSION     DAECOD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.233"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..887)
                     /locus_tag="KKO15_004044"
                     /pseudo
     CDS             complement(1..887)
                     /locus_tag="KKO15_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            895..1401
                     /gene="tnpA"
                     /locus_tag="KKO15_004045"
                     /pseudo
     CDS             895..1401
                     /gene="tnpA"
                     /locus_tag="KKO15_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1419..>1819)
                     /locus_tag="KKO15_004046"
     CDS             complement(1419..>1819)
                     /locus_tag="KKO15_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBD6362621.1"
                     /translation="DKILNYTNTEMKEAIAQRKPVVTTAGPRSLIFRGAITGVDTSKE
                     GLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLN
                     NESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          430 a          453 c          425 g          511 t
ORIGIN      
        1 tttaaacctg attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac
       61 tttgacatgt tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg
      121 gccgttgtga ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagttgc
      181 tgtattttgc gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt
      241 cgctgaagtc tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact
      301 gtgccatctg acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct
      361 gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacgtaccg
      421 caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct attccggtag
      481 cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc
      541 tgcgggtagc ggaatgcatc attctgtcac cgctttttga atcggggaaa agcagcccgc
      601 ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt
      661 gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa
      721 gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt ctgacgtgcc
      781 agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt
      841 tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata
      901 ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt tttcctgatg
      961 catgttcacc tagtctttgt caccagatac cgacgccaga tttttgacca tgacgcgaca
     1021 gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa
     1081 atggatggcg aaccagatca cgtccatttg ttaattaact aacctcccaa actggcgata
     1141 tccagtctgg taaacagcct caaaggcgta tcgggtaggt tactgcgacg agatcgacca
     1201 gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt
     1261 agctgcggag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct
     1321 ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac
     1381 cggataaaaa gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac
     1441 atggtggcat cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc
     1501 gtactttcgt tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta
     1561 ttagtcgctg catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg
     1621 cctgtagcca tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc
     1681 agcccttctt tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc
     1741 ccagcggtgg taaccactgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta
     1801 tagttcaaaa ttttatcca
//
LOCUS       DAECOD010000232         1809 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.234, whole genome shotgun sequence.
ACCESSION   DAECOD010000232 DAECOD010000000
VERSION     DAECOD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.234"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            173..1198
                     /locus_tag="KKO15_004047"
     CDS             173..1198
                     /locus_tag="KKO15_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6362622.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="KKO15_004048"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="KKO15_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          506 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAECOD010000233         1717 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.235, whole genome shotgun sequence.
ACCESSION   DAECOD010000233 DAECOD010000000
VERSION     DAECOD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.235"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(110..577)
                     /locus_tag="KKO15_004049"
     CDS             complement(110..577)
                     /locus_tag="KKO15_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD6362623.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="KKO15_004050"
     CDS             complement(643..882)
                     /locus_tag="KKO15_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBD6362624.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="KKO15_004051"
     CDS             complement(1017..1622)
                     /locus_tag="KKO15_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6362625.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          379 c          305 g          484 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAECOD010000234         1602 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.236, whole genome shotgun sequence.
ACCESSION   DAECOD010000234 DAECOD010000000
VERSION     DAECOD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.236"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(335..481)
                     /locus_tag="KKO15_004052"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="KKO15_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="KKO15_004053"
     CDS             535..717
                     /locus_tag="KKO15_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBD6362626.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="KKO15_004054"
     CDS             complement(818..1147)
                     /locus_tag="KKO15_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBD6362627.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRILL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1602)
                     /locus_tag="KKO15_004055"
     CDS             complement(1319..>1602)
                     /locus_tag="KKO15_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362628.1"
                     /translation="FVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          367 c          376 g          423 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggatgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa at
//
LOCUS       DAECOD010000235         1597 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.237, whole genome shotgun sequence.
ACCESSION   DAECOD010000235 DAECOD010000000
VERSION     DAECOD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.237"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..636)
                     /locus_tag="KKO15_004056"
     CDS             complement(<1..636)
                     /locus_tag="KKO15_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD6362629.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KKO15_004057"
     CDS             complement(750..1322)
                     /locus_tag="KKO15_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBD6362630.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          476 a          337 c          317 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacaactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAECOD010000236         1589 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.238, whole genome shotgun sequence.
ACCESSION   DAECOD010000236 DAECOD010000000
VERSION     DAECOD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.238"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            79..351
                     /locus_tag="KKO15_004058"
     CDS             79..351
                     /locus_tag="KKO15_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362631.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            351..656
                     /locus_tag="KKO15_004059"
     CDS             351..656
                     /locus_tag="KKO15_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362632.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            883..>1589
                     /locus_tag="KKO15_004060"
     CDS             883..>1589
                     /locus_tag="KKO15_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6362633.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAW"
BASE COUNT          525 a          345 c          389 g          330 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa
      781 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
      841 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      901 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      961 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
     1021 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
     1081 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctgg
     1141 cttcaacatt tcgcaggaat acaactaaga gacattactg aatcaaaaat ttattcagca
     1201 atgcagaaaa tgacgaaccg gcgtcatgag gaaaactgga aactcagggc agaatcatgc
     1261 agaaaaaaag ggaaacctgt tccagaatac acgccaaaac cagcgtccgt tgcaacgaag
     1321 gctacgcatc tttcatttat aaaggcccta ctaagagccg cagagcgtga atggaaaatg
     1381 ctggataagg caccaattat taaagtgcct caaccaaaga ataaacggat ccgctggctg
     1441 gagccccatg aagcacaaag gctgattgat gaatgtccgg agccattaaa gtctgttgtt
     1501 gaatttgcac tggcaacagg cttaagacgc tcgaacatca tcaaccttga atggcaacaa
     1561 atagatatgc agcgccgggt ggcatggat
//
LOCUS       DAECOD010000237         1558 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.239, whole genome shotgun sequence.
ACCESSION   DAECOD010000237 DAECOD010000000
VERSION     DAECOD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.239"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            12..299
                     /gene="cusF"
                     /locus_tag="KKO15_004061"
     CDS             12..299
                     /gene="cusF"
                     /locus_tag="KKO15_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux system protein CusF"
                     /protein_id="HBD6362634.1"
                     /translation="MGFNAQANEHHHETMSEAQPQVISATGVVKGVDLESKKITIHHD
                     PIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIKVSQ"
     gene            315..1034
                     /locus_tag="KKO15_004062"
                     /pseudo
     CDS             315..1034
                     /locus_tag="KKO15_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1240..>1558
                     /locus_tag="KKO15_004063"
     CDS             1240..>1558
                     /locus_tag="KKO15_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD6362635.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
BASE COUNT          408 a          374 c          391 g          385 t
ORIGIN      
        1 tgtttaccgt tattggcttt aatgcccagg ctaacgaaca tcatcatgaa accatgagcg
       61 aagcacaacc acaggtcatt agtgccaccg gtgtggtaaa aggtgttgat ctggaaagca
      121 aaaaaatcac catccatcac gatccgattg ctgccgtcaa ctggccggag atgaccatgc
      181 gctttaccat caccccgcag acgaaaatga gtgaaattaa aaccggcgac aaagtggcgt
      241 ttaattttgt ccagcagggc aacctttctt tattacagga tattaaagtc agccagtaac
      301 ccaggtttaa tgagatgaaa aaaatcgcgc ttattatcgg cagcatgatc gcgggcggta
      361 ttatttcagc gtcaggtttt acctggtttg accaggcgga accgcacgca gaaaaaatgc
      421 cgaccgcaga acgtaaaatc ttattctggt acgacccgat gtatcccaat acgcggttcg
      481 ataaaccagg taaatcgccg tttatggata tggatctggt gccgaaatat gccgatgaag
      541 agagttctgc gtctggtgtg cgcattgacc cgactcagac gcagaatctg ggggtgaaaa
      601 cggctaccgt cacgcgcggg ccgctgactt ttgcccagag tttcccggcg aatgtcagtt
      661 acaacgagta tcagtatgcc attgtgcagg ctcgcgctgc cgggtttatc gacaaggtgt
      721 atccgcttac cgtgggcgat aaagtacaaa agggcacacc gcttctcgac ctgaccattc
      781 ccgactgggt ggaagcgcag agtgagtatt tactgttgcg cgaaaccggc ggtacggcga
      841 cccagactga aggcattctt gagcggctgc gactggcggg aatgccggag gcggatattc
      901 gccacctgat cgccacgcaa aaaatccaga ctcgctttac gctcaaagcg cccattgatg
      961 gcgtgatcac cgcgtttgat ctgcgcgcgg gaatgaatat cgccaaagat aacgtggtcg
     1021 cgaaagagga aggtaggttg cttatctgtg tctgtcattg tctagcattg tccactgtag
     1081 ttaattaact gtctgttata actcgaaaaa caaccctttt ttcacctgag tatgtctacg
     1141 aacgtcaaat atagttgttt actgtgcgct gttgtccatg tgatattgtg taccagatgt
     1201 gtaccaaacc attatttctg agcgtaccaa atattattta tggctataag cgacacaaaa
     1261 ctgcgtacta tttatggtaa accatattct ggcccacaag aagtggctga tgctgatggc
     1321 ctcagcgtac gaatttcacc gaagggggtc atccagttcc agtaccgcta tcgctggcat
     1381 ggcaagccta atcgacttgg gcttggtcga tacccatccc tgtctttgaa ggatgccaga
     1441 cagatcactg ctgacttgcg aaagctctat ttctcaggaa cggatccacg cacttatttt
     1501 gaagagaagg tggagaactc catgacggtc gcccagtgtc tcgactactg gttcgaca
//
LOCUS       DAECOD010000238         1556 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.240, whole genome shotgun sequence.
ACCESSION   DAECOD010000238 DAECOD010000000
VERSION     DAECOD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.240"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1199)
                     /locus_tag="KKO15_004064"
     CDS             complement(<1..1199)
                     /locus_tag="KKO15_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6362636.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRK
                     TLLVHQAS"
     gene            1291..1377
                     /locus_tag="KKO15_004065"
                     /pseudo
     CDS             1291..1377
                     /locus_tag="KKO15_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          417 a          295 c          433 g          411 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagcttt cagtggcatc agcagcaaca gcgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggaaaagg tgttggcgtg ctcttcatgt
      241 tcaaaagcat gccatatctg tgatacatca gattgtttgt tttccgggaa ccatgctgtc
      301 acggcatcag ccagggggcg atggagtgta ttctgttgtc cgtcactcat ggagaagtaa
      361 atccgtgggc cgtggtagtc cggcgaagag gttaatcttt gcagggcttg cagagtgcga
      421 gtcgacaatg gattgttata aatattaaca tctgcataaa tgtgcagatt catgatactg
      481 tctggcaatg tgttcagctg attgttaggg atatatagtg attgcagacg ttgtggaagt
      541 tcgggcagtc tgtgtagttg gttttcccca acgtttaaaa gcctcagatt ttgaggaagg
      601 ggcggtagct cttgtaatct gttatcaaaa gcacttagtt cctgtagttc acatggtaat
      661 tctggtaagg cagtaagctg gttgttgcca acagaaagtt tttgtagagc cgggggaaga
      721 tcaggtagcg tctccaggct attgagaagg gctgagagtg actgcaagga agatggaaga
      781 gaaggcaaac atgttaaaac tctgttaagt gaaacatcaa gagcgaccag gtgaggcgga
      841 agagccggga gcctgctcag tctgttatcg ctggctttaa gtacagttaa ggacggaggg
      901 agttctggca gagagcgtag ctcattgcta gagatgttaa gttcttgtat gtgcgggggc
      961 aggtatggga gagagcgtaa tctgagtaaa cttaaattga gggctggctc ttgaaaagcc
     1021 agacatattt tcagtaatcg aactgcctgt gttcggtctt ctgttgcagc accttccttg
     1081 gcccagttat cccagatgcg gtgataatcc gtgatatttt gctcttctac ggataaacgg
     1141 gatatacatc ctaaaccaca aaaggagcgg aaaaaagaag gattgtgggc aggtttcatt
     1201 tattattgat cctcattgtt tatatattaa tatattcgtg caaatggagt gaggattctt
     1261 taaaaaaacg cgcctgtaaa tagttaagaa caggaagaac tgttggggat gtggctggcc
     1321 gaaaatgaag gggtgaagtt ctggctcaat gtgctgactg aactgaaaaa acgcggttgg
     1381 tcgccctacc ttttcatcaa gccagtagtc tgcccaccac agcatcattt ctctgcgctt
     1441 atcgagatac tgagcatggt tgtaaattca gctaacccag tgcgttgaca aaaaattagc
     1501 gcaagaggac aaaaaatcac cttgcgctaa tgctctgtat caggtcacta atacca
//
LOCUS       DAECOD010000239         1549 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.241, whole genome shotgun sequence.
ACCESSION   DAECOD010000239 DAECOD010000000
VERSION     DAECOD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.241"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            351..476
                     /locus_tag="KKO15_004066"
     CDS             351..476
                     /locus_tag="KKO15_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBD6362637.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="KKO15_004067"
     CDS             complement(921..1397)
                     /locus_tag="KKO15_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6362638.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          357 a          405 c          385 g          402 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggt gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcctc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcaccct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAECOD010000240         1496 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.242, whole genome shotgun sequence.
ACCESSION   DAECOD010000240 DAECOD010000000
VERSION     DAECOD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.242"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..201)
                     /locus_tag="KKO15_004068"
     CDS             complement(<1..201)
                     /locus_tag="KKO15_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362639.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDPEEDYEE"
     gene            598..774
                     /locus_tag="KKO15_004069"
     CDS             598..774
                     /locus_tag="KKO15_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362640.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLMREYSD
                     DTAHDVSGLNTFIR"
     gene            complement(1082..1390)
                     /locus_tag="KKO15_004070"
     CDS             complement(1082..1390)
                     /locus_tag="KKO15_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6362641.1"
                     /translation="MAMSYSYELQAQVYDHMTELEGGKDINLLDFSGLTDMAISEMQN
                     RVAAAEKFSFEMHGQAGSALMTRRKKEKKAIKKAEQLVKDLIQFKLCDMGDFPDGKPA
                     "
BASE COUNT          370 a          402 c          327 g          397 t
ORIGIN      
        1 ctcttcataa tcctcttccg gatcaccatc aggcacatcg tttttacttc ttctcagata
       61 cgatatgtaa aaatcacgcc aggcatccag atccagttgt cctcgcttgt tcgaaaccgg
      121 ggcacccggc agtttctgta atctgcgaag ctggcgatcg gtcagactca aatgcctggc
      181 aacttcagtc tgagtagcca ctgttcacct cgccaaaaaa tctcccctca tgagttttaa
      241 acatgcatct tgcgaacaac tttatgaaac gcggcatata tgtccggttt gagtgttcaa
      301 tttttgcgca tgtccggttc gcagaaagcg catttctata tttttcaaat ggttaacctt
      361 cagagaaacc ggacatggtt tccgaaaaat tttcataaat agtgaaattc tgcgccgctc
      421 ccgccccgtg gcaggccacc ccaccgggag gacccgtcag cctgacagcc atgacgaacg
      481 tctgatacag cgccctgcat gaatgacatc gggataatcc agaaaggaat agcatcgtac
      541 ccacaagaat cagtgtaagt gtcctgtttc ttccaccccc gcacaggact ggcgagcatg
      601 agggacaaac ccgcgaacca taaacgcggt aaaaacccgg tgtgcatcgt ttttgattat
      661 tcccgcacac tcgcgcagaa ggagttcccc gtcgggctac ggtcatggtt aatgcgggaa
      721 tacagcgacg atacagcgca tgatgtgtca ggcctgaata cctttatccg ttaaaaggga
      781 tatcagttaa gttatcccgt gtagggtata agccattaca aagcccacca gtagatgggc
      841 tttgtaatga agagccgttg tgaaagtagc tctcaggttg tcattttttc ccttttctgc
      901 ctacctgccc actgttttgc aatgtacagg cagtcatcga aaactcggcc ttttctgctg
      961 gcttgtgaac aacggcggta atgctccacc gccatgtcag cgccagtcat agccgcgttc
     1021 tggtcgtacc caagctttat taactcggtt gttacatttt tatgtatgaa atcaatcgga
     1081 gtcatgctgg tttaccatca gggaaatccc ccatgtcaca tagcttgaac tgaatcagat
     1141 ccttcacaag ctgctcagcc tttttaatgg ccttcttctc tttcttccga cgagtcatga
     1201 gagcgctacc tgcttgaccg tgcatttcaa atgagaactt ctcagcagcc gcgacacggt
     1261 tttgcatttc gctgattgcc atatcggtca ggccagagaa atcgaggagg ttaatgtcct
     1321 tcccgccctc aagctccgtc atgtggtcat acacctgcgc ctgaagctca tagctgtagc
     1381 tcattgccat gaggcaagct tcacgcttgg gaaaattgca aatatccctc tccacaacac
     1441 cacctgttcc gttgatatag gtatcagttg caaaaaattt tgcagctgca tccccg
//
LOCUS       DAECOD010000241         1492 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.243, whole genome shotgun sequence.
ACCESSION   DAECOD010000241 DAECOD010000000
VERSION     DAECOD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.243"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..904)
                     /locus_tag="KKO15_004071"
     CDS             complement(<1..904)
                     /locus_tag="KKO15_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362642.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYY"
     gene            complement(901..1429)
                     /locus_tag="KKO15_004072"
                     /pseudo
     CDS             complement(901..1429)
                     /locus_tag="KKO15_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          481 a          359 c          290 g          362 t
ORIGIN      
        1 aatagtaaat taagcacggt acatagcaga gtaaaaaata ggcttcaaac caaatccgcc
       61 attccacaag cgttgccaat accgacacca gcgtaatcca catcatttca ataacccgca
      121 atatgaataa ccgaagccaa agcggcttgg caaaaaaggt cgacaacgta ttttcactgg
      181 agaaaaacag atcgatttcc tgcaaaaaaa acctgcccag aaaagcgggc cgcttggaga
      241 agcgcagaaa aaacagatta atcgccaaca ctacgccaca aagaatgagc aatgcaatta
      301 tcgatttaaa cgttaaccac tgcccctctg tcacaaaaga atcactgtta cgcaacgggc
      361 tgatagtaag caggttaaca accgttgaga tgcacaccga aagcagcaca tagcgagtca
      421 gacttttata ttttactttt ttcgctactg aaatagcgac ctgcttgaca taactggtgc
      481 ggatcatccg aaacagcaga aagatgacca acggcccaag taacgaaatc aggaacagcg
      541 catataacga gggtagaagc tggtatagcc cccaactcat tgcactcaac atgattaacg
      601 acagaaatcc tgccaccaga ggttgtgtaa gaaataattc tattttgtgg ttctgctttt
      661 tcttaaacca cgaaaaatcg atattcttcg tgacgcattt ataagcccag tacgccttga
      721 gatcaaaaat aaaattatac agcaccaggg ccagaatccc actaactaca ccaatatcct
      781 ctgatggaaa agtaaaccct gcatttttcc aataaaagcc tatcagaata agtccaatag
      841 cataaatgta tatcagattt cgtttatcct gaaagcgaat aaaagttaaa taatcaggaa
      901 tcatttgaat acctttgcat cactaaatgc tttacgacaa tgcataactc gttgctgatc
      961 cacgccagca aaatcattta acatattccc caaatagggc ttattactta tttcaaggaa
     1021 agctaccggg gttggcatat tcagaatatg tatcgagaga ttcagtccat ttcaccgtat
     1081 gagttaagtg taatgagaga ttttctttga taattgacgg agcgacctcg ctttttgctg
     1141 ttacgttcat cagaacctga tgctctggcg aagcgatatc cagtccggca agataatcgc
     1201 gcattgcctg aacgccgtct tccatcaaac gcgtgtgcca ggcaccgctc acaccgagtt
     1261 taaccggttc gtatccagcg gccatcagca gcgtggtaaa ttcattcaac gcagcctgcg
     1321 ttcccccaat aacctgctgg cgcggcgtgt tatcacagct aatatccagc gcaatgcctg
     1381 attccgtaat catcgtctgc agttgttcgc ggttgatgcc tttaaccgcc tgatcttacc
     1441 cagcaatagt ggacacgcgg ctaagtgagt aaactctcag tcagaggtga ct
//
LOCUS       DAECOD010000242         1473 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.244, whole genome shotgun sequence.
ACCESSION   DAECOD010000242 DAECOD010000000
VERSION     DAECOD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.244"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            35..997
                     /gene="ldtC"
                     /locus_tag="KKO15_004073"
     CDS             35..997
                     /gene="ldtC"
                     /locus_tag="KKO15_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBD6362643.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1080..1292)
                     /locus_tag="KKO15_004074"
     CDS             complement(1080..1292)
                     /locus_tag="KKO15_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6362644.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          385 a          358 c          355 g          375 t
ORIGIN      
        1 attacaataa ccttatattt attatgtatt tttggtgatg atcaaaacgc gtttttctcg
       61 ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg gcgctaccgg caaaagccaa
      121 cacctggccg ctgccgccag cgagcagtcg tctggttggc gaaaacaaat ttcatgtggt
      181 ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc
      241 tctgttacaa gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac
      301 gatcccgttg caaaccctac ttccagacgc gccgcgcgaa ggcattgtga tcaacattgc
      361 ggagctgcgt ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg
      421 tattggtcag ttaggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa
      481 acgtgcaaac ccaacctgga cgccaacggc aaacatccgc gcccgttata aagcacaggg
      541 aattgagttg cctgcggtag tgccggctgg accggataac ccaatgggcc atcatgcgat
      601 tcgtctggcg gcctatggcg gcgtttattt gcttcacggt acgaacgccg atttcggcat
      661 tggcatgcgg gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt
      721 tagccaggtc accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc
      781 cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga
      841 cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc
      901 acaaactgac gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga
      961 tgtccgccgt catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaaatccccg
     1021 cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct
     1081 tatttataaa ttactgctgt tccatggagg gtattcggac cggttacaga agtaatacgg
     1141 aaagattttg cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga
     1201 tttgtccccg cgttagcact gatagtaccg actttttgtt ggccttctgg cgttgactga
     1261 acttcgacag ccgcaaagtt ggcaaatgac atggagctta aaatcgccgc agcgatgagg
     1321 tttttacgtt tttcataata gtggccttat gcagatgaat gacgtcgcaa gaaattactt
     1381 aacgatcgat aactaaatga tagatgtgat ctggatcaca tacaagatat tttttataac
     1441 aatcattaat taatttaaaa atctttaaat ttc
//
LOCUS       DAECOD010000243         1402 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.245, whole genome shotgun sequence.
ACCESSION   DAECOD010000243 DAECOD010000000
VERSION     DAECOD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.245"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..391
                     /locus_tag="KKO15_004075"
     CDS             <1..391
                     /locus_tag="KKO15_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD6362645.1"
                     /translation="WTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERN
                     IKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDC
                     RIRSNNCYGQVIRRDQESALACWGIDQ"
     gene            388..855
                     /locus_tag="KKO15_004076"
                     /pseudo
     CDS             388..855
                     /locus_tag="KKO15_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            843..992
                     /locus_tag="KKO15_004077"
                     /pseudo
     CDS             843..992
                     /locus_tag="KKO15_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1074..1361
                     /locus_tag="KKO15_004078"
     CDS             1074..1361
                     /locus_tag="KKO15_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362646.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT          368 a          312 c          379 g          343 t
ORIGIN      
        1 atggaccatc tgtcgtggtg ccacaatggt ggatggtaag cccgtcatac cgggaatgaa
       61 gctgtcgaag gaaaaatgcg accaggttaa cgctattgaa cgtgataagg cgctggcatg
      121 ggtggagcgc aatattaaag taccactgac cgaaccacag aaagcgggta tagcgtcatt
      181 ttgtccctat aacattggcc caggtaagtg tttcccgtcg acgttttata agcggctgaa
      241 tgccggtgat cgtaagggcg catgcgaggc gattcgctgg tggataaaag atggtgggcg
      301 cgattgccgc atacgttcaa ataactgcta tggacaggtt attcgtcgtg accaggaaag
      361 cgcattagcc tgttggggga tagatcagtg agctgagtcg ccgcgattat ttatgctctg
      421 gttatctgca tcatcgtctg cctgtcgtgg gcggtcaatc attaccgtga taacgccatc
      481 gcctacaaag aacagcgtga taaaaaagtc agtgagctga agctggcgac cgccaccatt
      541 actgacatgc agcaacgcca gcgtgctgct gatgtactcg atgctaaata cacgaaggag
      601 ttggctgatg cgaaagctga aaatgatgct cttcggcgca agcttgataa tggtggccgg
      661 gtgctcgtca aaggaaaatg ctctgtgcca tcctcagccg aaacctccag cgcctccggc
      721 atgggcaatg atgccaccgt cgaactctct ccagttgctg gacgaaacgt tctcggtatc
      781 cgggacggaa tcatcagaga ccagacagca ctgagaacgc ttcaggaata tatcaggacg
      841 caatgccttc gatgatagag ataattttac tcatcatcct tcacatctgg ctctgtagac
      901 agggtggtga tcacttctgg agtgaatcca gattaaacat ctcattgctg atgcttaaag
      961 ttgagcattt ggcgcgcggt aaggggctgc gtttagataa gagccagtca tcacaaacac
     1021 caggatttag cctcgcattc gcggggcttt ttattgcaac aaaggtaaag acgatggatg
     1081 aagaatatcg taaagacctg caactgtggt ttggtcttac gcatgcgtcg ttctgcgtga
     1141 tgccgcgagt attcatggag gctatgcctc cagaatggca agagaagatg gctcagttgc
     1201 tttttgagta tagcgacacg atcaaaacgg atgtctgcgg agttcacagc tgttttgtta
     1261 ctgccaaaga cggcaacaac cgctttatga ggatgccaga agatattctg aactatcgtc
     1321 atcctcgacg tgaattcatc gaatcatttc tgaagaagta gccattacaa agcctatcta
     1381 cgggtgggtt tgataatggc tt
//
LOCUS       DAECOD010000244         1295 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.246, whole genome shotgun sequence.
ACCESSION   DAECOD010000244 DAECOD010000000
VERSION     DAECOD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.246"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..340
                     /locus_tag="KKO15_004079"
     CDS             <1..340
                     /locus_tag="KKO15_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362647.1"
                     /translation="PVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARY
                     KYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYY
                     RPLQKLINGT"
     gene            364..908
                     /locus_tag="KKO15_004080"
                     /pseudo
     CDS             364..908
                     /locus_tag="KKO15_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            932..>1295
                     /locus_tag="KKO15_004081"
     CDS             932..>1295
                     /locus_tag="KKO15_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD6362648.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSRPKMT"
BASE COUNT          368 a          278 c          341 g          308 t
ORIGIN      
        1 gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc agagaaaccc
       61 ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt acggaaactt
      121 cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg gccatgaaat
      181 gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt cgggacacgg
      241 gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg ttgacggtgt
      301 gtattaccga ccactgcaaa aactgattaa tggcacctag tataacgtag cgagtattta
      361 acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga acaatatcag
      421 atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa ctggtatgaa
      481 gagctgaaga atttcgccag gacacaatta aaataactta caccggagac ggtcgcgtgg
      541 tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt attgaagttc
      601 ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac cgcaatgatg
      661 aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt ctggctcagg
      721 tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc agcgacgaag
      781 accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg atggatttca
      841 gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct ccggaaattt
      901 cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag gtgcgctgac
      961 tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc gggtaaacac
     1021 cccggaagtg gtgatgcaca ctgttgctga tgtgatgaca ggaaacgatg gtgaatactg
     1081 tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc acaacgagta
     1141 caacgttggc gacattgctg tatatgagga ctcaaagccc ggtacgctga atgactttct
     1201 gattgctcct gatgagggcg acctgaaacc agatgtcgtc aaacgctttg aggaaatggt
     1261 ggcgcaggcg cagcagagca ggccaaaaat gacat
//
LOCUS       DAECOD010000245         1278 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.247, whole genome shotgun sequence.
ACCESSION   DAECOD010000245 DAECOD010000000
VERSION     DAECOD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.247"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            139..375
                     /locus_tag="KKO15_004082"
     CDS             139..375
                     /locus_tag="KKO15_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD6362649.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGV"
     gene            complement(391..>1278)
                     /locus_tag="KKO15_004083"
     CDS             complement(391..>1278)
                     /locus_tag="KKO15_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6362650.1"
                     /translation="RKAVRLRYAFIRDNSCCWPVRLLCRVLDVHPSGFYAWLQQPHSQ
                     RHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVG
                     YRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRK
                     IIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEG
                     SMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSS
                     EQMSPTEYENQYYQRLGSV"
BASE COUNT          338 a          330 c          262 g          348 t
ORIGIN      
        1 gatttttgat attaatatct ttcaccatcc gctattttca ccacttcaga aaacaaactt
       61 atttttatta aaatcaattc tttttctttg aacattcaac tgcgatattt gtaaaaacat
      121 taaacaggca acaataaaat gacattacca acaactattt attcattccc cgcatattta
      181 ccccgttttt cgtccaccga caaaccagca aaattaaaat ttcatcaata cgcacgcgct
      241 acattactgt caaatcgtgg aagagatcat aattgcgatg gcagacgtac tgttgagatc
      301 cacaaactag atctctctga ctggcaagca ttcaataagt tagcaacgaa gtgtaatgct
      361 tacgatggag tatgaatcgc cacggataat ctagacactt ccgagccgtt gataatactg
      421 gttttcatat tctgtcggtg acatctgttc gctagaacca tgccgacgct tactgttata
      481 aaacatttcg atgtaatcaa aaatatcact gcgggcttct tcccgcgttc cgtagatctt
      541 tttctttatc cgttcacgtt tcaacaactg gaaaaaactt tctgcaaccg cattatcatg
      601 gcagttaccg cgacggctca tgctaccctc caggccgtgt gatttcagga acgactgcca
      661 ctcatggctt gtgtactgac tgccctgatc cgaatgaacc agcacctgtt tttcgggatt
      721 acgccgccat acagccatca gcagtgcgtt caggacaatg tcctttgtca tccgggattg
      781 catggaccag ccgataattt tgcgtgagaa cagatcaaca acaacggcaa gatacagcca
      841 gccttcgtgg gtcctgatgt aggttatgtc cgttacccaa cgctcatcag gagcatccgg
      901 attgaactgt cgctggagcc tgttgggtga cacgatactg gcctcgcctt tacgtgcccg
      961 cgggcttcgg tatccgacct gagcctttat tccgacacgt ttcatcagtc tccagactct
     1021 gtttactccg cactgttgcc cgctgtcacg cagatccaga tggattttgc gataaccata
     1081 gacgcatccc gattccagcc agaactgttt aatctgtcct gtcagtctca ggtctgcctg
     1141 atggcgttgt gaatgcggct gctgaagcca ggcgtaaaaa ccactgggat gaacatccag
     1201 cacccgacag agcaggcgaa caggccagca acaggagttg tcacggataa aggcgtacct
     1261 cagtcggaca gctttgcg
//
LOCUS       DAECOD010000246         1237 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.248, whole genome shotgun sequence.
ACCESSION   DAECOD010000246 DAECOD010000000
VERSION     DAECOD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.248"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            119..>1237
                     /locus_tag="KKO15_004084"
     CDS             119..>1237
                     /locus_tag="KKO15_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD6362651.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     ALTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQVLI"
BASE COUNT          267 a          390 c          322 g          258 t
ORIGIN      
        1 gcgctggtaa ccgctaatag gggtcaggtc aggcactttt gccgggaccg tctgtaacgt
       61 ggatgccggt acctgctccc cgtggttatc tggttaaccc atatacaagg gagacagaat
      121 gaccgaatcc agcgattacg aatccgtcca ggtctttatc ggcgttgatg tcggtaaaga
      181 tacgcatcac gctgttgcca ttaatcgttc aggtaaacgc ctgttcgata aagcattacc
      241 caacgacgaa aacaaactca ggtcgctaat atctgacctg aaacaacatg gtcagatact
      301 gctggttgtt gatcagccag ctaccatcgg tgcgttacct gtcgccgttg cccgctcaga
      361 aggagtcctt gtcggatacc tccctggact ggccatgcgc cgcatagccg acttacacgc
      421 cggtgaagct aaaactgatg ctcgtgacgc tgccatcatt gccgaagctg cccgtaccct
      481 gcctcacgcg ctacgcacgc tgaaactggc tgacgagcaa atcgccgaac tctccatgct
      541 ctgcggcttc gatgatgatc ttgccgcaca gacaacgcag gccagcaacc gtatccgcgg
      601 ccttctgacc cagatacatc cggcaccgga gcgcgttctc ggtccgagac ttgagcaccc
      661 ggcggtactc gatcttctcc agcgatatcc ctcaccagaa aaactcgctt cgctgggtga
      721 gaagaagctg gcagcccagc tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc
      781 agacatagct caggcactgg ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc
      841 cgtagtactg ccacgtctgg cactccagct catcacgctg cgtaagcaaa gagacgaggt
      901 ggcgcttgag gtagaacagc gagttcttgc tcaccctctt tacccggccc tgaccagtat
      961 gcccggagtc ggtgtcagga ccgcagccag actcctcacc gaggtcgcct gccgcgcctt
     1021 cgcctctgcc gcacatctcg ctgcttatgc tggccttgcg ccggtaactc ggcgatccgg
     1081 ctcgtcaata cgcggtgagc atccctcgcg acggggtaat aaagctctca aacgggcgtt
     1141 gttcctgtcg gccttcgccg cgctcaggga tccgctctcc agggcttact acacccgcaa
     1201 aatgagtcag ggaaaacgac acaatcaggt gcttatc
//
LOCUS       DAECOD010000247         1222 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.249, whole genome shotgun sequence.
ACCESSION   DAECOD010000247 DAECOD010000000
VERSION     DAECOD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.249"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            502..924
                     /locus_tag="KKO15_004085"
     CDS             502..924
                     /locus_tag="KKO15_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBD6362652.1"
                     /translation="MRQTEDKQHQNIEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            902..1174
                     /locus_tag="KKO15_004086"
     CDS             902..1174
                     /locus_tag="KKO15_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBD6362653.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHH"
BASE COUNT          389 a          279 c          312 g          242 t
ORIGIN      
        1 aaagaagcac cgattggtat cgaggtcatc cccgcgcacg tcactgaata tctgaacaag
       61 gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt
      121 cgctcctctg ccccgatgcc gcagcgtgta acagaagaag gaaaacagga tgatgaagaa
      181 aaaccgcagc catctggcgc aatggcagat gaacaggcaa cggctgaaac aatggaaccg
      241 gatgcaactg aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct
      301 gttgatgaga aatatcagga actgcgggaa gaactccatg aagcccggaa aaacattccg
      361 cccaaaaatc ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa
      421 gggattagcg acccgaatga tccgaaatgg attcccggtc atcatatctc ctcaaatgaa
      481 gtcaaaaaaa cggaaaatga agtgcgacag actgaagata aacagcacca gaacatcgaa
      541 ccggaggagg gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg
      601 gtgatgggag acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag
      661 gaagaagagc cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc
      721 gatcagcata atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag
      781 gtgttgattg cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct
      841 gcaatccctg acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt
      901 gatgagcgct tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca
      961 gcagatcgtt cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc
     1021 tcagcacctg tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca
     1081 ggcaatcagc cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat
     1141 ccggtacatg gctgaaacca ttgcccacca ctaggttgat attgatttgg aggactaaaa
     1201 tctatggcgc tttttcaacg ag
//
LOCUS       DAECOD010000248         1219 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.250, whole genome shotgun sequence.
ACCESSION   DAECOD010000248 DAECOD010000000
VERSION     DAECOD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.250"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(38..481)
                     /locus_tag="KKO15_004087"
     CDS             complement(38..481)
                     /locus_tag="KKO15_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD6362654.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            638..>1219
                     /gene="metA"
                     /locus_tag="KKO15_004088"
     CDS             638..>1219
                     /gene="metA"
                     /locus_tag="KKO15_004088"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD6362655.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRY"
BASE COUNT          285 a          357 c          263 g          314 t
ORIGIN      
        1 cgtcttctcc agcttgggca ggcacggcag gggcggctta ttcccccaca tacgccagat
       61 tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta accttaaaac
      121 ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg gttgtcagtt
      181 ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag ccgcacacat
      241 caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac ccaaccggct
      301 gctcccgttc attaaccgcg acccacaacg gtgcttccgg cagaaaggaa cgcaccagct
      361 cttccagctc ggcccgatac tctgctgata aaaaatcgtg ggtggcatcg acagatcgac
      421 accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata ctaataacca
      481 ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga aactttctca
      541 ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct atctggatgt
      601 ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg tgccggacga
      661 gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa cttctcgtgc
      721 gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc cgaagaagat
      781 tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg tcgatattca
      841 gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc tgaacaactt
      901 ctactgtaac tttgaagata ttcaggaaca gaactttgac ggtctgattg taaccggtgc
      961 gccgctgggc ctggtggagt ttaatgatgt cgcttactgg ccgcagatca aacaggtgct
     1021 ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg tacaggccgc
     1081 gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct ctggcgttta
     1141 cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg attcattcct
     1201 ggcaccgcat tcgcgctat
//
LOCUS       DAECOD010000249         1202 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.251, whole genome shotgun sequence.
ACCESSION   DAECOD010000249 DAECOD010000000
VERSION     DAECOD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.251"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>1202
                     /locus_tag="KKO15_004089"
     CDS             join(<1..299,299..>1202)
                     /locus_tag="KKO15_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBD6362656.1"
                     /translation="PEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWR
                     KQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRGKK
                     VDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHH
                     VIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCL"
BASE COUNT          295 a          294 c          365 g          248 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg
      781 cactgggcgg tgactaccgg cggcttcaac agtgaaacag tacaggacgt catgctggga
      841 gcggtggaac gccgcttcgg caacgatctt ccgtcgtctc cagtggagtg gctgacggat
      901 aatggttcat gctaccgggc taatgaaaca cgccagttcg cccggatgtt gggacttgaa
      961 ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag cttcgtgaaa
     1021 acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
     1081 aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc gctgggttat
     1141 cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga taacagatgt
     1201 ct
//
LOCUS       DAECOD010000250         1174 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.252, whole genome shotgun sequence.
ACCESSION   DAECOD010000250 DAECOD010000000
VERSION     DAECOD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.252"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(183..1091)
                     /locus_tag="KKO15_004090"
     CDS             complement(183..1091)
                     /locus_tag="KKO15_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362657.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          230 a          312 c          286 g          346 t
ORIGIN      
        1 ttgctgactc cccgattgtt gatgagcgcc ggacctcgcg caacgctttc ccagaggcag
       61 ttttgccttc cccgagcggt cacgcaaaac gttgcaacga cctgatcatt tgcagttttt
      121 agcggaacgc agatataaaa aatccccggc tttttgtgcc agggacgttc atagctgaaa
      181 atttatttta cgttttcggc gacgggagtt gttgagatta agggtgaagt caatgccgcc
      241 agtgaaccgc ctgaaacgcc aatttcattg agcaaggagt caatcagcgg agcctgtgtg
      301 cggtaagaga gagcggctga taatgcttgt tccgctaggt ttccgccccc cacattaccc
      361 gtgtttgcat cacccgcagc gctgccacta ttcagaccat cgacctgaat aatcttgata
      421 ccgtcaatag atttcatcgg ctcaacggat ttttctatta ccgcaggtag cgcctgcaac
      481 aaggccagtt tgaatttaag gctggtttgt tcatcagaaa gtacgttgat agcatcgttc
      541 agcgcacgtt gtgcttctgc ttccgccaga ccctttttac gtgtagcttc ggctaactca
      601 acgatagccg ccgcctgcat ctctgcggct tctttttctg ctttcgcccg cacggtcagt
      661 tcaaccgctt tggtttctgc atcctgcgcg gcagcgatta gggcaacttg tttagcacga
      721 tcggcttctg cggtctggcg agtggtttct acgttttgtt gcgcacttac tgcttctgcg
      781 agtgcaaggt tagcacgcgc ttctgcctgg gactgttgtt ccgatttggc ggcaatagcg
      841 atcgatttcg tctggttggc gatttcggtg acctgctgct gttcgatctc tttaatacga
      901 acttcacgtt cagcctcaac ctttcttgag cggacggcct gctcgcgctc gatctccgtt
      961 tcctgaattt gtcgttcagc cagaattcgc gtctgctctg cttcacgacg acgttcagct
     1021 tcaaaagccg caatacgtgc attctgttcg gcagtacggg ttttaacctg ctgctcctgc
     1081 tcaagcgtca taaacgcttc ttgttgttcg gtaatgactc caacttactg atagtgtttt
     1141 atgttcagat aatgcccgat gaccttgtca tgca
//
LOCUS       DAECOD010000251         1161 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.253, whole genome shotgun sequence.
ACCESSION   DAECOD010000251 DAECOD010000000
VERSION     DAECOD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.253"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            115..>1161
                     /gene="cadB"
                     /locus_tag="KKO15_004091"
     CDS             115..>1161
                     /gene="cadB"
                     /locus_tag="KKO15_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBD6362658.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASD"
BASE COUNT          247 a          255 c          298 g          361 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagtcgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga c
//
LOCUS       DAECOD010000252         1156 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.254, whole genome shotgun sequence.
ACCESSION   DAECOD010000252 DAECOD010000000
VERSION     DAECOD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.254"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            2..385
                     /locus_tag="KKO15_004092"
     CDS             2..385
                     /locus_tag="KKO15_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6362659.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="KKO15_004093"
     CDS             complement(386..1030)
                     /locus_tag="KKO15_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6362660.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          262 c          278 g          321 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaat
//
LOCUS       DAECOD010000253         1092 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.255, whole genome shotgun sequence.
ACCESSION   DAECOD010000253 DAECOD010000000
VERSION     DAECOD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.255"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1001
                     /locus_tag="KKO15_004094"
                     /pseudo
     CDS             1..1001
                     /locus_tag="KKO15_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          308 a          266 c          257 g          261 t
ORIGIN      
        1 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
       61 cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg
      121 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgct cctgactttt
      181 tctgctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt
      241 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
      301 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa ccatggacta
      361 cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg
      421 atatctggac ctgatatgca aatcatcaaa atctctgcaa cccactgtat aacctaatgt
      481 atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
      541 gacaaggatt atggctctgt cacgttatca aaccggatcg ctgggctgct atgtgcgcac
      601 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc
      661 atcggaaaat cctcaaacca gaacatttag actggcaaga atatgcatta ttgctgctca
      721 atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact
      781 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
      841 atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg
      901 cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
      961 gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt
     1021 tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc gcatggcgat
     1081 ccatcaggtt ag
//
LOCUS       DAECOD010000254         1068 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.256, whole genome shotgun sequence.
ACCESSION   DAECOD010000254 DAECOD010000000
VERSION     DAECOD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.256"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            18..560
                     /locus_tag="KKO15_004095"
     CDS             18..560
                     /locus_tag="KKO15_004095"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362661.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="KKO15_004096"
     CDS             787..>1068
                     /locus_tag="KKO15_004096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBD6362662.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          201 c          257 g          296 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgtgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAECOD010000255         1066 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.257, whole genome shotgun sequence.
ACCESSION   DAECOD010000255 DAECOD010000000
VERSION     DAECOD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.257"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(570..749)
                     /locus_tag="KKO15_004097"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KKO15_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="KKO15_004098"
     CDS             843..>1066
                     /locus_tag="KKO15_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6362663.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAECOD010000256         1021 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.258, whole genome shotgun sequence.
ACCESSION   DAECOD010000256 DAECOD010000000
VERSION     DAECOD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.258"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(50..190)
                     /locus_tag="KKO15_004099"
     CDS             complement(50..190)
                     /locus_tag="KKO15_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362664.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="KKO15_004100"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="KKO15_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBD6362665.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAECOD010000257         1007 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.259, whole genome shotgun sequence.
ACCESSION   DAECOD010000257 DAECOD010000000
VERSION     DAECOD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.259"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..371
                     /locus_tag="KKO15_004101"
     CDS             <1..371
                     /locus_tag="KKO15_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362666.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
     gene            374..919
                     /locus_tag="KKO15_004102"
     CDS             374..919
                     /locus_tag="KKO15_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD6362667.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            943..>1007
                     /locus_tag="KKO15_004103"
     CDS             943..>1007
                     /locus_tag="KKO15_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD6362668.1"
                     /translation="MSVVISGALTDGAGIPMSGYH"
BASE COUNT          286 a          219 c          262 g          240 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gtgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctgg tataacgtag
      361 cgagtattta acaatgttgc atttaaaaaa tattactgcg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag cgacacaata aaaatagctt acaccggaga
      541 tggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatc gagccacgca atgccagcgt
      601 tatcgaagtt ccggatatta ccgctaaccg acggattacc gcgccgggtt actggtttta
      661 ccgcaatgat gaatttgtct ttgactacag actcaaagcg gaagatgagc gtgatgccct
      721 tctggctcag gtcagtgccc ggacagggga atgggaagaa gacctgctgc tggggttaat
      781 cagcgacgaa gataaagaaa agctgaaagc ctgtcgtatt tacgcgaaat cgctgcaggc
      841 gatggatttc agcaccatca ctgataaatc ctcatacaac gccattgaat ggcccgcctc
      901 tccggaaggt tcttcctgat ttaatttatc gcgagaaaaa caatgtctgt agtgatatca
      961 ggtgcgctga ctgatggcgc aggtatcccc atgtccggat accatat
//
LOCUS       DAECOD010000258         1005 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.260, whole genome shotgun sequence.
ACCESSION   DAECOD010000258 DAECOD010000000
VERSION     DAECOD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.260"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..217
                     /locus_tag="KKO15_004104"
     CDS             <1..217
                     /locus_tag="KKO15_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362669.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(397..>1005)
                     /locus_tag="KKO15_004105"
     CDS             complement(397..>1005)
                     /locus_tag="KKO15_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD6362670.1"
                     /translation="AQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRDQA
                     VPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRTEQE
                     AKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMPELEE
                     LAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          220 a          299 c          253 g          233 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgcctgaa aacggctcac aactgcacca ttcataatca catcgcataa accacagacc
      241 ggactgactc cggaaaaaca gaggcccacc cccgggcctc cccggattca tccgtttctc
      301 tgttcagcct gaccgcacac cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag
      361 cggtcttctg tgattccgcc agttgcggct tattcattac ttaacgtcaa acgcccgaat
      421 tgaagccaaa tcatccagac tgctcagctc ctctttcatc tcccgctgac ggcgataaat
      481 ctcatcgttg cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg
      541 catcgacagt ttcacctgct gattatccgc atcactccac accatatgtg tttgtgccgc
      601 gacagatttt gccagcatga ccaccgggga caggcgggcc actgacgagg cgtcagcgtt
      661 ccacgtgcgg ccgttccatt caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc
      721 ttcaatttcc tgacgtctgg cctctctggc cgcttccagc atttctggtg tcacagtgaa
      781 tggggctatc tcaccccatt tgccactttg cagttcctgc cagatttgct gacccgtcgg
      841 tgcaacatca tcagcggtgg ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac
      901 gtcacagtct actgcgccac tttcggtata acggggatta atgatttttt taatttccac
      961 agtgcattcc tcacgatgtg cgaataaaaa gcccgggcat tgcgc
//
LOCUS       DAECOD010000259         1003 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.261, whole genome shotgun sequence.
ACCESSION   DAECOD010000259 DAECOD010000000
VERSION     DAECOD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.261"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..734
                     /locus_tag="KKO15_004106"
     CDS             <1..734
                     /locus_tag="KKO15_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6362671.1"
                     /translation="PFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVY
                     TGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDAL
                     KSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESW
                     TSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDN
                     NHEQIELLNRRLSESVPCMPHKKAG"
     gene            755..>1003
                     /locus_tag="KKO15_004107"
     CDS             755..>1003
                     /locus_tag="KKO15_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBD6362672.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          322 a          225 c          228 g          228 t
ORIGIN      
        1 atccatttaa cggtctgaca ccattaaaaa agtccaagat agaaccagat cccctcaccc
       61 gtgacgaatt tattcgtttt attgaggctt gccgtcatca acaaacaaaa aacctgtgga
      121 ttatcgctgt atacacgggt attcgtcacg gggagctggt atcactggcc tgggaagata
      181 tagaccttaa agcgaggact ataaccatcc gccggaatta tacaaaacta ggcgaattca
      241 ctccaccaaa aactgatgct ggtaccggaa gaacgattca tctagttcaa ccagctattg
      301 atgctcttaa aagccaagcg gaaatgacca tgcttggaaa acagcattct gtagaggtaa
      361 agcagaggga atatgggaga agtactgtgc ataaatgcac ttttgttttt agtcctcagg
      421 taataaaaca gcggcagttt tccggaccgc actataaggt tgactccatc agggagtcat
      481 ggacaagtat cttaaaacgc gcaggtctga gacacagaaa atcgtatcaa tccaggcata
      541 cctatgcatg ctggtcactt gccgcaggag ctaatcctag ttttatcgca agccagatgg
      601 gccacacaaa cgcacaaatg gtattcaatg tttacggagc atggatgaaa gacaacaatc
      661 acgaacagat agaacttctt aacagaagac tatctgaaag tgtcccatgc atgccccata
      721 agaaagcggg gtaaaataaa aacttgcaaa atcaattggt ttacctttaa tccctgtcac
      781 gttacgcgcg tggcagaggc gttacggatt gctgaaaccg caacggacag acggcggtca
      841 tcggctgttc aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa
      901 cggcgtgcag gtcagcaaag ttaaaatact gctcagtaat gaaaatgttg atgtgcagaa
      961 cgtctggcgc gatcagcaag aaacattact gacttacctg caa
//
LOCUS       DAECOD010000260          983 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.262, whole genome shotgun sequence.
ACCESSION   DAECOD010000260 DAECOD010000000
VERSION     DAECOD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.262"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KKO15_004108"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KKO15_004108"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBD6362673.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAECOD010000261          977 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.263, whole genome shotgun sequence.
ACCESSION   DAECOD010000261 DAECOD010000000
VERSION     DAECOD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.263"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            99..287
                     /locus_tag="KKO15_004109"
     CDS             99..287
                     /locus_tag="KKO15_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362674.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            297..>977
                     /locus_tag="KKO15_004110"
     CDS             297..>977
                     /locus_tag="KKO15_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBD6362675.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVL"
BASE COUNT          208 a          271 c          283 g          215 t
ORIGIN      
        1 tgcaaattga aaaccgcccc tgttcgtcct gttgttgccg cacgctccgc agaatatgta
       61 tgctctgagg acctgtttta ttaaggactc atcctcttat gttgacccgc aaaagtatag
      121 ataccgttct gctctctgtt ggtgctgaga agctctcaca acgtgaatgg gactggatga
      181 agatgctcaa acccatggac ccaccacctg cgatggtcgc ggcctccatc ctggagcgcc
      241 gtggcgacac agcagccctt acccggctac aggataccgg gggttaatcc gtcctcatgt
      301 tgtccgcttt tactccccgc ccgctgaaac gtctgttcac ggccaaccag tgctggacat
      361 ccttcctgga tgcgggcggt ctgcgcgata tcgaggttga ggctgtcaca aaaatgctgg
      421 cctgcggcac gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc
      481 acgtcagata cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg
      541 ccacagacct gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc
      601 tggtcttcac cctgccggac acgcagtggc cggtgttcga aagcaaccgg tggctgctga
      661 atgacgtgtg ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg
      721 aacccggtat cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg
      781 tacatgtgtc tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct
      841 tcctgaaaga cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag
      901 cgtggtcaga ggggctggcg atgccggagt cgttgtcaca tatcacgacg gaatcacaga
      961 ggagaagcct ggtgctg
//
LOCUS       DAECOD010000262          976 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.264, whole genome shotgun sequence.
ACCESSION   DAECOD010000262 DAECOD010000000
VERSION     DAECOD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.264"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..199
                     /locus_tag="KKO15_004111"
                     /pseudo
     CDS             1..199
                     /locus_tag="KKO15_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..925
                     /locus_tag="KKO15_004112"
                     /pseudo
     CDS             299..925
                     /locus_tag="KKO15_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          304 a          228 c          219 g          225 t
ORIGIN      
        1 ttggctacgt aattaaaaca gaaaataaag aaaccccatt cactgtagtt gatatcgacg
       61 gtctatcagg aaactttaaa actcttgttg aaggtgccac aaaaatgtgc ctagtttaca
      121 tcggaaataa tctgcccgca gaaaaaaagc cgaatttctg gcaaatctga ttgcaatgaa
      181 attaaaaggt gaaatctgaa aaaaagaaag cctgcacagt gtgcaggcct gagtgaagaa
      241 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
      301 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      361 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      421 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      481 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      541 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      601 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      661 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      721 tcttctgcac atttttacgt atgctgccgg ggatcggaaa tacctgcatc atgccacccg
      781 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      841 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      901 ccgcctgact gatgcaatta aggctgaggt gtactggcaa tagcggacac taccatttgt
      961 tcttttttta agcagc
//
LOCUS       DAECOD010000263          972 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.265, whole genome shotgun sequence.
ACCESSION   DAECOD010000263 DAECOD010000000
VERSION     DAECOD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.265"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..473)
                     /locus_tag="KKO15_004113"
     rRNA            complement(<1..473)
                     /locus_tag="KKO15_004113"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          223 a          265 c          211 g          273 t
ORIGIN      
        1 aaaggtatta actttactcc cttcctcccc gctgaaagta ctttacaacc cgaaggcctt
       61 cttcatacac gcggcatggc tgcatcaggc ttgcgcccat tgtgcaatat tccccactgc
      121 tgcctcccgt aggagtctgg accgtgtctc agttccagtg tggctggtca tcctctcaga
      181 ccagctaggg atcgtcgcct aggtgagccg ttaccccacc tactagctaa tcccatctgg
      241 gcacatccga tagcaagagg cccgaaggtc cccctctttg gtcttgcgac gttatgcggt
      301 attagctacc gtttccagta gttatccccc tccatcgggc agtttcccag acattactca
      361 cccgtccgcc actcgtcagc gaaacagcaa gctgtttcct gttaccgttc gacttgcatg
      421 tgttaggcct gccgccagcg ttcaatctga gccatgatca aactcttcaa tttaaaagtt
      481 tgatgctcaa agaattaaac ttcgtaatga attacgtgtt cactcttgag acttggtatt
      541 catttatcgt cttgcgacgt taagaatccg tatcttcgag tgcccacaca gattgtctga
      601 taaattgtta aagagcagta ccgcttcgct ttttctcagc ggcgcggggt gtgcataata
      661 cgctttcccg ctacagagtc aagcatttat ttttgctttc tctgccggaa ttctcaggag
      721 aaccccgctg acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag
      781 ggagttattc cggcctgaca agcgaaaaat ataaaaactt tatcgttcgc tcacttttca
      841 ggcaaaacat cttaaatata gtcttttccg tctaacttat agacaaaaac gagccccgaa
      901 gggctaatgc cgatcagtta aggatcagtt gaccgatcca gtggctgtgt aagaatccgg
      961 aaacgctcac tt
//
LOCUS       DAECOD010000264          931 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.266, whole genome shotgun sequence.
ACCESSION   DAECOD010000264 DAECOD010000000
VERSION     DAECOD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.266"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(53..241)
                     /locus_tag="KKO15_004114"
     CDS             complement(53..241)
                     /locus_tag="KKO15_004114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362676.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          179 c          217 g          290 t
ORIGIN      
        1 gcgctgatgg ttcttatccg ttttattgaa tcggagaaca aactgtgagc tatcagcctg
       61 gtggtctggt tttgcctgat ttaagccgcc gtgggtattt gtccaagaat gcttcaagct
      121 ccaatcgttt tggcctggtg aatggagatg ggttaagcaa agactctcgt tgctggtgta
      181 ctttttcgcg ctggcgctca agcatggata atgcgattgc ctgtagttct tcgttagtca
      241 ttgtctgcac ctggaaagca atttaccgga gtacctcaca ctacggcaaa aatgttttat
      301 tgttgcaact tgcgtaatta ttcaggtcag tttggaacgc actgtataaa cataaataac
      361 ctgatactgt tgtgtgtttt caaagaaaac acaggtattt ttcacataaa ccttcccctt
      421 ttcatattcg accacttaat caaaaaacgg ttttatactt gttgccccca ctggggttaa
      481 atgtgagaca tgtcgcactt tgtgccatgt gtcctcgctg attttaggcg cggttcattc
      541 attctttctg ttggcataaa gtatggtatc ggttgggcga atgaaaggga ctcgggtcat
      601 ggtcagacaa tacctgaggc gttaattcgt tgaaagagat tgtgaatggg ggaataactg
      661 agtcttaact gctgtgcgat agcccggaca gatagttgct cgtcgaatct gagacgtaat
      721 gattctggca aataagtgcg aaagccggtg atatctagtg ttgtttgaga gttcatcgtt
      781 cgcgtccatt aatacagatg gacgcgattg ttgccagaca ggataatttt cacaagacat
      841 cgcagataga gtacttacga taatttttta ttttatacaa cagatgccca cgaaaagaat
      901 tattttcaat agacacctgc attacacaaa g
//
LOCUS       DAECOD010000265          832 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.267, whole genome shotgun sequence.
ACCESSION   DAECOD010000265 DAECOD010000000
VERSION     DAECOD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.267"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>832
                     /locus_tag="KKO15_004115"
     CDS             <1..>832
                     /locus_tag="KKO15_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD6362677.1"
                     /translation="PVLPACIKKLDVSCNKLTILTDLPENIKELIARDNFLTHISALP
                     HYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPS
                     LPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQI
                     TTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLR
                     QMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA
                     "
BASE COUNT          262 a          195 c          125 g          250 t
ORIGIN      
        1 tacctgtact ccctgcttgc attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc
       61 ttactgattt acctgaaaat attaaagaac ttattgcaag agataatttc ttaacacata
      121 tatctgcatt accacattat ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc
      181 tgccgttatt accagacacc atcaaatcac taagcgcaga gtataatagg ttatccacac
      241 tgccttcatt acccttgaat ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc
      301 ttccatctct gccttctaat cttaagatac ttaaggttgc gcacaaccat cttactgaac
      361 tgcccccttt acctaggaga ctgcaacttc tttttgcata tagcaataga ttaagcaact
      421 taccaaacat ccaagaaaat attatcatga gaagattttt ttattttgaa aacaaccaaa
      481 taactacaat cccgacaaat ctttttcgtt tagatcctca tataactatt gagattgcaa
      541 ataacccctt atcagatcaa actctgctat tcttaataca gcaaacttcg gttccaaatt
      601 ttaacgggcc tcagtttcgt atttccctgt cagaccaaaa cagactgttt ttacgccaga
      661 tgttgccgca aaatttacat tcgcgccata tcagagtcat cactgaaggg gggcagaact
      721 ttcagatccc ccctcttccc gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg
      781 aggtttctac acaatggact tctttttcca ccgaggagaa ttcccgggca tt
//
LOCUS       DAECOD010000266          831 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.268, whole genome shotgun sequence.
ACCESSION   DAECOD010000266 DAECOD010000000
VERSION     DAECOD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.268"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..326
                     /locus_tag="KKO15_004116"
     CDS             <1..326
                     /locus_tag="KKO15_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD6362678.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KKO15_004117"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KKO15_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBD6362679.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAECOD010000267          758 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.269, whole genome shotgun sequence.
ACCESSION   DAECOD010000267 DAECOD010000000
VERSION     DAECOD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.269"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>758
                     /locus_tag="KKO15_004118"
     CDS             <1..>758
                     /locus_tag="KKO15_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD6362680.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          183 g          209 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagca
//
LOCUS       DAECOD010000268          686 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.270, whole genome shotgun sequence.
ACCESSION   DAECOD010000268 DAECOD010000000
VERSION     DAECOD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.270"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>686
                     /locus_tag="KKO15_004119"
     CDS             <1..>686
                     /locus_tag="KKO15_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBD6362681.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSL"
BASE COUNT          216 a          121 c          164 g          185 t
ORIGIN      
        1 atttaagttc agcagcagtt aaaacagcag gtgtacttat cagctttcag gctgtactat
       61 ccgcttatca gcgagtaatg gaagttggcc ttcaacgtgc aagtgctgaa cgttcaattg
      121 atttcgtatt ttctaaagat gcaaatcaag taaaagattt cacaaagtca ttagctcaaa
      181 caacgggttt agatatcgct gaactgcaat cacagtttgc tggtttcggt gcttcagcta
      241 gagagtctct aggcattcaa ggttctgaag aactttttag aaatatgatt ggctattctc
      301 gtctgatggg gcgaagtgaa gaagagatta agcgagcact gacagcatta tcacagatgg
      361 ctggtaaggg tcagattatg gctgaagagc ttaaggggca actagctgaa gctgttcctg
      421 gtatggttca ggtattcgct aaagcaaccg gaaagactga acaggaatta tttgatgcta
      481 tgaagaaggg ggctttaaaa tctgccgata ctcttcagaa ggtcactcag gaactgaata
      541 aacaaatcac cgctaaaggt ggctggaaag ctatttcaga aagtacacag gcacaactag
      601 ggaacttgaa aaactcttgg aataccacgc ttgatagtat cttcagaggt tctgaaaatg
      661 gtctgcaaga ttttacaaga agctta
//
LOCUS       DAECOD010000269          669 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.271, whole genome shotgun sequence.
ACCESSION   DAECOD010000269 DAECOD010000000
VERSION     DAECOD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.271"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..103
                     /locus_tag="KKO15_004120"
     CDS             <1..103
                     /locus_tag="KKO15_004120"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; catabolic; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBD6362682.1"
                     /translation="LDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            125..>669
                     /locus_tag="KKO15_004121"
     CDS             125..>669
                     /locus_tag="KKO15_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD6362683.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSL"
BASE COUNT          137 a          158 c          166 g          208 t
ORIGIN      
        1 attagaccaa tatattcaaa acagaaaaac cgtcagtatt atttccggcg gcaatatcga
       61 tctttctcgc gtctctcaaa tcaccggttt cgttgacgct taattaattc gttgaggata
      121 ggatatgagt acttcagata gcattgtatc cagccagaca aaacaatcgt cctggcgtaa
      181 atcagatacc acatggacgt taggcttgtt tggtacggca atcggcgccg gggtgctgtt
      241 cttccctatc cgcgcaggtt ttggcggact gatcccgatt cttctgatgt tggtattggc
      301 ataccccatc gcattttatt gccaccgggc gctggcgcgt ctgtgtcttt ctggctctaa
      361 cccttccggc aacattacgg aaacggtgga agagcatttt ggtaaaactg gcggcgtggt
      421 tatcacgttc ctgtacttct tcgcgatttg cccactgctg tggatttatg gcgttactat
      481 taccaatacc tttatgacgt tctgggaaaa ccagctcgga tttgcaccgc tgaatcgcgg
      541 ctttgtggcg ctgttcctgt tgctgctgat ggctttcgtc atctggtttg gtaaggatct
      601 gatggttaaa gtgatgagct acctggtatg gccgtttatc gccagcctgg tgctgatttc
      661 tctgtcgct
//
LOCUS       DAECOD010000270          669 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.272, whole genome shotgun sequence.
ACCESSION   DAECOD010000270 DAECOD010000000
VERSION     DAECOD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.272"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..525
                     /locus_tag="KKO15_004122"
                     /pseudo
     CDS             1..525
                     /locus_tag="KKO15_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          187 a          135 c          162 g          185 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagc
//
LOCUS       DAECOD010000271          620 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.273, whole genome shotgun sequence.
ACCESSION   DAECOD010000271 DAECOD010000000
VERSION     DAECOD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.273"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            78..620
                     /locus_tag="KKO15_004123"
                     /pseudo
     CDS             78..620
                     /locus_tag="KKO15_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          144 a          158 c          165 g          153 t
ORIGIN      
        1 gatggggcgc ttacaaacga atcggaaaat cgatactgga aaaactctaa ctcggtggcc
       61 agtttttgtt gaccacttca ggtcaggaga ttgtcgtcga accgattgaa caggctattc
      121 tcatctctga gcagactaag ctgtgggacg ccaagtcatc caccggattc actcagggca
      181 cacttcagca ggacgctatg aacatctgtg agaatgtggt ccgtaccatc aacgctcgca
      241 tggtcgacgt tctggaatcc tctaagctgc tgaagaccgt agagcttcct gtcctgactg
      301 gtagcctgac cgctaaggca gacgctatca tggacgctct gtatgagaac accgagcctt
      361 ctttcggttc tgaggtgtct gactacggta ttatcgccca tgagagccaa ctgaaagcac
      421 tgtcccgtct ggctgctaaa cagggcttct ctggtgaaga cgctatcgtg gatatgctgg
      481 gtaccgacat cgcgtattac aacggtgagg acaaaggtgt gttcatgttg gctaaacgct
      541 tcacggccct gagcttcggc tgcttccgtc acgacggtga gaatattacg gtcgtccttt
      601 cacgtgatgg cgactctcag
//
LOCUS       DAECOD010000272          619 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.274, whole genome shotgun sequence.
ACCESSION   DAECOD010000272 DAECOD010000000
VERSION     DAECOD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.274"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>619
                     /locus_tag="KKO15_004124"
     CDS             <1..>619
                     /locus_tag="KKO15_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HBD6362684.1"
                     /translation="RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRT
                     PDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLI
                     EQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKL
                     KTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT          162 a          176 c          180 g          101 t
ORIGIN      
        1 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
       61 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      121 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      181 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      241 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      301 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      361 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      421 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      481 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      541 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      601 catgcgcttc cccggagga
//
LOCUS       DAECOD010000273          619 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.275, whole genome shotgun sequence.
ACCESSION   DAECOD010000273 DAECOD010000000
VERSION     DAECOD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.275"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..>619)
                     /locus_tag="KKO15_004125"
     rRNA            complement(<1..>619)
                     /locus_tag="KKO15_004125"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          188 c          146 g          154 t
ORIGIN      
        1 gtgatccaac cgcaggttcc cctacggtta ccttgttacg acttcacccc agtcatgaat
       61 cacaaagtgg taagcgccct cccgaaggtt aagctaccta cttcttttgc aacccactcc
      121 catggtgtga cgggcggtgt gtacaaggcc cgggaacgta ttcaccgtgg cattctgatc
      181 cacgattact agcgattccg acttcatgga gtcgagttgc agactccaat ccggactacg
      241 acgcacttta tgaggtccgc ttgctctcgc gaggtcgctt ctctttgtat gcgccattgt
      301 agcacgtgtg tagccctggt cgtaagggcc atgatgactt gacgtcatcc ccaccttcct
      361 ccagtttatc actggcagtc tcctttgagt tcccggccgg accgctggca acaaaggata
      421 agggttgcgc tcgttgcggg acttaaccca acatttcaca acacgagctg acgacagcca
      481 tgcagcacct gtctcacggt tcccgaaggc acattctcat ctctgaaaac ttccgtggat
      541 gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa accacatgct ccaccgcttg
      601 tgcgggcccc cgtcaattc
//
LOCUS       DAECOD010000274          566 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.276, whole genome shotgun sequence.
ACCESSION   DAECOD010000274 DAECOD010000000
VERSION     DAECOD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.276"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..297
                     /locus_tag="KKO15_004126"
     CDS             <1..297
                     /locus_tag="KKO15_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBD6362685.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="KKO15_004127"
     CDS             complement(405..>566)
                     /locus_tag="KKO15_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6362686.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAECOD010000275          553 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.277, whole genome shotgun sequence.
ACCESSION   DAECOD010000275 DAECOD010000000
VERSION     DAECOD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.277"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..>553)
                     /locus_tag="KKO15_004128"
     CDS             complement(<1..>553)
                     /locus_tag="KKO15_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HBD6362687.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          119 c          135 g          165 t
ORIGIN      
        1 ccatttaata tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc
       61 ttcctggacg atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc
      121 aagtgccgtg agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat
      181 ctgactgatc gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa
      241 tgtggctgtt ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa
      301 tttgtctgct tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac
      361 aacttcgggc ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt
      421 atttttgtca gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga
      481 taagtccgct accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt
      541 cgcttgctcc aca
//
LOCUS       DAECOD010000276          519 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.278, whole genome shotgun sequence.
ACCESSION   DAECOD010000276 DAECOD010000000
VERSION     DAECOD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.278"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..94
                     /locus_tag="KKO15_004129"
     CDS             <1..94
                     /locus_tag="KKO15_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBD6362688.1"
                     /translation="DWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            124..477
                     /locus_tag="KKO15_004130"
                     /pseudo
     CDS             124..477
                     /locus_tag="KKO15_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          150 a          120 c          125 g          124 t
ORIGIN      
        1 ggactggttg cgcgaagtga tcgagaagct caatgactgg ccgtcgaacc aagtgcatga
       61 actgctgccc tggaacttct cgtctgtaaa ataatcctta cgctacgtac ttctcggggc
      121 gcttacgaac aaattaagcc ttttctcgaa aaggccatga caatgtacaa tgatgttctt
      181 ttccctggag aagacataaa caaaccgatc agcatcgctg cagccaaccg attcgtaaat
      241 agaataaggg gagggatgga tctaggttac tggcgaacac atgatttcag aagaacgctt
      301 gttacacggc tgtccgagat gaatgtcgag cctcatgtta ctgagcgaat gctcggtcat
      361 gaacttggcg ggataatgtc cgtatacaat aaacacgact ggatagaggc tcagcgcaaa
      421 gcgtatgagc ttcacgctga taaattgttc tggcacatca ggagcatttc tgattaacgc
      481 caccgttaag aatccaccct tcaacagctt cacgaaggt
//
LOCUS       DAECOD010000277          506 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.denovo.279, whole genome shotgun sequence.
ACCESSION   DAECOD010000277 DAECOD010000000
VERSION     DAECOD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.denovo.279"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>506
                     /locus_tag="KKO15_004131"
     CDS             <1..>506
                     /locus_tag="KKO15_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBD6362689.1"
                     /translation="KELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCG
                     DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMF
                     HSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSF
                     SEAAHAIT"
BASE COUNT          120 a          123 c          161 g          102 t
ORIGIN      
        1 aaagagctgg ggctggtcag ctgtcagcag ccgactcacc ggtataaacg tggtggtcat
       61 gaacatgttg ctatccctaa ctaccttgaa aggcagttcg ccgtgaccga gccaaatcag
      121 gtgtggtgcg gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt
      181 gttctcgacc tgttcgcaag aaaaccagtg ggctgggcca tgtcgttctc gccggacagc
      241 aggctcacca tgaaagcgct ggaaatggca tgggaaaccc gtggtaagcc cggcggggtg
      301 atgttccaca gcgatcaggg cagtcattat acgagcaggc agttccggca gttattgtgg
      361 cgataccaga tcagacagag tatgagccgg cgcggaaact gctgggataa cagcccaatg
      421 gaacgcttct tcaggagtct gaagaacgaa tggatgccgg tggtgggtta cgtaagcttc
      481 agcgaggcag ctcacgccat aacgga
//
LOCUS       DAECOD010000278         3999 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.guided.193, whole genome shotgun sequence.
ACCESSION   DAECOD010000278 DAECOD010000000
VERSION     DAECOD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.guided.193"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..238)
                     /locus_tag="KKO15_004132"
     CDS             complement(<1..238)
                     /locus_tag="KKO15_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD6362690.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDM"
     gene            500..958
                     /gene="gpt"
                     /locus_tag="KKO15_004133"
     CDS             500..958
                     /gene="gpt"
                     /locus_tag="KKO15_004133"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD6362691.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1050..2294
                     /gene="frsA"
                     /locus_tag="KKO15_004134"
     CDS             1050..2294
                     /gene="frsA"
                     /locus_tag="KKO15_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD6362692.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2352..2753
                     /gene="crl"
                     /locus_tag="KKO15_004135"
     CDS             2352..2753
                     /gene="crl"
                     /locus_tag="KKO15_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD6362693.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2792..3847)
                     /gene="phoE"
                     /locus_tag="KKO15_004136"
     CDS             complement(2792..3847)
                     /gene="phoE"
                     /locus_tag="KKO15_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD6362694.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1000 a          959 c          981 g         1059 t
ORIGIN      
        1 ccatatcgag gtgggcctgt aagacgaccg gtttacgatt ttccatacct gcggtagcag
       61 gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct ttctcttttg
      121 cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga ggaatagaac
      181 agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt tcagacacgt
      241 taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat
      301 gttgtcaaca caagacaggc ttgcgagata tgtattgaga ataccacttt atcccgcgtc
      361 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
      421 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
      481 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
      541 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
      601 cgtaagccgt ggcggtctgg taccgggggc gttactggcg cgtgaactgg gtattcgtca
      661 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
      721 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
      781 tggtactgct gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
      841 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
      901 gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
      961 ttttcaacgc ctggcattgc cgggcgttgt tctttttaac ttcaggcagg ttacaatagt
     1021 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     1081 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     1141 cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga
     1201 ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc gacgtccagg
     1261 cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat accgtgattg
     1321 gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa
     1381 aagcctgtac ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt
     1441 tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg
     1501 ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga
     1561 tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg
     1621 atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt
     1681 acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc
     1741 cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc
     1801 acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg
     1861 gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga
     1921 aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc
     1981 aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt
     2041 ccgacgaagc gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg
     2101 ggcgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg
     2161 aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat
     2221 ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa
     2281 aacgcttgtg ttaaaaattt gctaaatttt gctaatttgg taaaacagtt gcatcacaac
     2341 aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta
     2401 ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt
     2461 gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt
     2521 ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg
     2581 ataaagcagg cgactggaag agtgttccgg taaaagatac tgaagtggtt gaacgactgg
     2641 agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga
     2701 agctggaacc ggcgaatgat tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat
     2761 gtgccggatg catcacatcc ggcaatattc attaaaactg atacgtcatc ccaaccgcga
     2821 cagtatcatc attattaata ttcagcttat tatcgctatc cagttggttg attttataat
     2881 caacaaacgc tgacatattt ttgttgaaat aatacgtagc accgacgtcg atataattga
     2941 ccagatcttc atcaccgatg ccctcaatat ctttcccttt cgataagaca taacccagcg
     3001 atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat
     3061 tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat
     3121 aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc
     3181 cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc ccactaatgg
     3241 cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct
     3301 ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga
     3361 taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt ttggtcataa
     3421 agttgtcggt ctgcgccgag gaatcgccac caaattccgg gaacatatcg gtccaggctt
     3481 ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa tctttatatt
     3541 tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc gctttatttc
     3601 cggcaaactc cgcttcccac cgaccataac cagtcagttg atcgttaatt tgagtttcac
     3661 ctttaaaacc aaaacggata taactctggt cgccatcttt actggcgtta tcactcatat
     3721 aatgcatggc tttaactttg ccatagacat ccagtttatt accgtcttta ttatatattt
     3781 ctgcagcctg tacagatgca gatgccacaa tgcccatcac cactaatgcc agagtgctct
     3841 ttttcatttt cattcctgat tttaattaac gcgcgaatat tcagcgggag agccccgttg
     3901 aaaacaggaa agtttttaac ctgagattgt taaagatata ttacagatta atgatattct
     3961 gaaaatgggt aatgctgcca acttactgat ttagtgtat
//
LOCUS       DAECOD010000279         3026 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1566 isolate 8
            SAMN14086353-rid12263703.guided.205, whole genome shotgun sequence.
ACCESSION   DAECOD010000279 DAECOD010000000
VERSION     DAECOD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086353
            Sequence Read Archive: SRR11285403
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043763.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 06:37:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,141
            CDSs (total)                      :: 4,052
            Genes (coding)                    :: 3,741
            CDSs (with protein)               :: 3,741
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 149 of 311
            Pseudo Genes (incomplete)         :: 61 of 311
            Pseudo Genes (internal stop)      :: 124 of 311
            Pseudo Genes (multiple problems)  :: 23 of 311
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086353-rid12263703.guided.205"
                     /strain="SFL1566"
                     /isolate="8"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..464
                     /gene="frdB"
                     /locus_tag="KKO15_004137"
     CDS             <1..464
                     /gene="frdB"
                     /locus_tag="KKO15_004137"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD6362695.1"
                     /translation="EALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQ
                     TPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERM
                     AQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            475..870
                     /gene="frdC"
                     /locus_tag="KKO15_004138"
     CDS             475..870
                     /gene="frdC"
                     /locus_tag="KKO15_004138"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD6362696.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            881..1240
                     /gene="frdD"
                     /locus_tag="KKO15_004139"
     CDS             881..1240
                     /gene="frdD"
                     /locus_tag="KKO15_004139"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD6362697.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1303..2436
                     /locus_tag="KKO15_004140"
     CDS             1303..2436
                     /locus_tag="KKO15_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD6362698.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2525..>3026
                     /gene="blc"
                     /locus_tag="KKO15_004141"
     CDS             2525..>3026
                     /gene="blc"
                     /locus_tag="KKO15_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD6362699.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVS"
BASE COUNT          728 a          781 c          788 g          729 t
ORIGIN      
        1 ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca
       61 tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg
      121 gtactaacat ccagaccccg gcgcagatgg caaagtatca ccagttctcc ggttgcatca
      181 actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc
      241 cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg
      301 agcgtatggc gcagttgaac agccagaacg gtgtatggag ctgtactttc gtgggctatt
      361 gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag ggcaaagtag
      421 aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg
      481 actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt
      541 tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa
      601 ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac
      661 tttttacaaa acccggttat cgtgatcatt aacctgatca ctctgacggc agctctgctg
      721 cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa
      781 aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc
      841 gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc caaatccaaa
      901 gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat
      961 tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc tgtttccggg
     1021 tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt
     1081 cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat
     1141 gcacgatctg aaaatccacg tgcctgcggg caaatgggtt ttctacggtc tggctgctat
     1201 cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca atgtaaatcc
     1261 ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa aacgacgctc
     1321 tgcgccttat taattaccgc ctcttactcc acatttgctg cccctcaaca aatcaacgat
     1381 attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg
     1441 gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa
     1501 aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg
     1561 ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccgcaaca
     1621 aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt acatcttgcg
     1681 acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc ctcaagcgac
     1741 ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca acgtctgtat
     1801 gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt
     1861 gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac gtggattaat
     1921 gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa ggcagtgcat
     1981 gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat tgaagatatg
     2041 gcccgctggg tgcaaagcaa tttacccccc cttgatatca atgagaaaac gcttcaacaa
     2101 gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc
     2161 tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag tgacaataaa
     2221 attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc agtacgcgca
     2281 tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc gtttattcca
     2341 gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc agcgagagtc
     2401 gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg
     2461 gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac
     2521 atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg
     2581 tagttttcct acgccgccgc gtggcgtgac cgtagtgaat aatttcgacg ccaaacgtta
     2641 tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa
     2701 agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta ataaaggcta
     2761 taaccctgac agaggaatgt ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc
     2821 aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt ataacgttat
     2881 tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg actacctgtg
     2941 gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc tggcagtcgc
     3001 gacccgggaa gggtttgatg tcagta
//
